treatments-xml/data/03/AF/87/03AF87D2C666FFE4E86F43D9FE92FF1D.xml
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<document id="A6341BF6FDDB8D2149996671E8C9C580" ID-DOI="10.1016/j.phytochem.2018.10.002" ID-ISSN="1873-3700" ID-Zenodo-Dep="10484732" IM.bibliography_approvedBy="karina" IM.illustrations_approvedBy="karina" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.tables_requiresApprovalFor="GgImagineBatch" IM.taxonomicNames_approvedBy="felipe" IM.treatments_approvedBy="karina" checkinTime="1704941929211" checkinUser="felipe" docAuthor="Aziz, Ahmad Nazif, Ismail, Nor Hadiani, Halim, Siti Nadiah Abdul, Looi, Chung Yeng, Anouar, El Hassane, Langat, Moses K., Mulholland, Dulcie &amp; Awang, Khalijah" docDate="2018" docId="03AF87D2C666FFE4E86F43D9FE92FF1D" docLanguage="en" docName="Phytochemistry.156.193-200.pdf" docOrigin="Phytochemistry 156" docSource="http://dx.doi.org/10.1016/j.phytochem.2018.10.002" docStyle="DocumentStyle:9E596C34F4E94307D29315B03ACE1007.6:Phytochemistry.2014-2019.journal_article" docStyleId="9E596C34F4E94307D29315B03ACE1007" docStyleName="Phytochemistry.2014-2019.journal_article" docStyleVersion="6" docTitle="Croton oblongus Burm. f. Bark" docType="treatment" docVersion="4" lastPageNumber="197" masterDocId="FF96FFAAC662FFE1EB5D426AFF92FFF7" masterDocTitle="Laevifins A-G, clerodane diterpenoids from the Bark of Croton oblongus Burm. f." masterLastPageNumber="200" masterPageNumber="193" pageNumber="197" updateTime="1706123863087" updateUser="karina">
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<mods:title id="892FC1D7A901B6C90D0B47BB9078E4F5">Laevifins A-G, clerodane diterpenoids from the Bark of Croton oblongus Burm. f.</mods:title>
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<mods:namePart id="A6729A75A2A04D19AF5D51D6B91DC497">Aziz, Ahmad Nazif</mods:namePart>
<mods:affiliation id="E36A6BCA4D75A073435F81DF73D2C4C8">School of Fundamental Science, Universiti Malaysia Terengganu, 21030, Kuala Nerus, Terengganu, Malaysia &amp; &amp; Atta-ur-Rahman Institute for Natural Products Discovery, Level 9, FF 3, Universiti Teknologi MARA Puncak Alam Campus, 42300, Bandar Puncak Alam, Selangor, Malaysia</mods:affiliation>
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<mods:namePart id="16104A4CB034A44EC3F08DAD0A30F17D">Ismail, Nor Hadiani</mods:namePart>
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<mods:namePart id="25DACAA1F0C7FA357D076A6AFBC9B2CF">Halim, Siti Nadiah Abdul</mods:namePart>
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<mods:namePart id="FA442513886162B24BE74D5A055042DA">Looi, Chung Yeng</mods:namePart>
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<mods:namePart id="9C7F3D95559E325927541AE2E628842E">Anouar, El Hassane</mods:namePart>
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<mods:namePart id="EA45733028A08844ED8682944CA0E5E7">Langat, Moses K.</mods:namePart>
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<mods:namePart id="19A66C6B0834498E131BE8360B395CCD">Mulholland, Dulcie</mods:namePart>
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<mods:namePart id="8DDE60B7AFF461CAB30699D116E7F16C">Awang, Khalijah</mods:namePart>
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<mods:title id="7D295ED5C76AC87F7F86D2B5CE53632B">Phytochemistry</mods:title>
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<mods:date id="745CF58822C58101536CA29CC7BDB5F7">2018</mods:date>
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<treatment id="03AF87D2C666FFE4E86F43D9FE92FF1D" LSID="urn:lsid:plazi:treatment:03AF87D2C666FFE4E86F43D9FE92FF1D" httpUri="http://treatment.plazi.org/id/03AF87D2C666FFE4E86F43D9FE92FF1D" lastPageId="5" lastPageNumber="197" pageId="4" pageNumber="197">
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<paragraph id="8BB936C4C666FFE5E86F43D9FAA5FE31" blockId="4.[818,1335,435,454]" box="[818,1335,435,454]" pageId="4" pageNumber="197">
<heading id="D0F181A8C666FFE5E86F43D9FAA5FE31" bold="true" box="[818,1335,435,454]" fontSize="36" level="1" pageId="4" pageNumber="197" reason="1">
<emphasis id="B972EAD6C666FFE5E86F43D9FAA5FE31" bold="true" box="[818,1335,435,454]" italics="true" pageId="4" pageNumber="197">
2.1. Biological activities of
<taxonomicName id="4C064D47C666FFE5EF7743D9FAA5FE31" ID-CoL="ZQQD" authority="Burm. f. Bark" authorityName="Burm. f. Bark" box="[1066,1335,435,454]" class="Magnoliopsida" family="Euphorbiaceae" genus="Croton" kingdom="Plantae" order="Malpighiales" pageId="4" pageNumber="197" phylum="Tracheophyta" rank="species" species="oblongus">Croton oblongus Burm.f. Bark</taxonomicName>
</emphasis>
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<paragraph id="8BB936C4C666FFE5E80E4381FA8BFD52" blockId="4.[818,1488,490,1123]" pageId="4" pageNumber="197">
It is known that inflammation is closely linked to tumour promotion and almost all tumours have inflammatory cells reside in them regardless of the underlying cause of the tumour (
<bibRefCitation id="EF974B35C666FFE5EFAB4048FA5BFDC2" author="Rosenberg, L. &amp; Palmer, J. R. &amp; Zauber, A. G. &amp; Warshauer, M. E. &amp; Stolley, P. D. &amp; Shapiro, S." box="[1270,1481,546,565]" pageId="4" pageNumber="197" pagination="355 - 358" refId="ref10635" refString="Rosenberg, L., Palmer, J. R., Zauber, A. G., Warshauer, M. E., Stolley, P. D., Shapiro, S., 1991. A hypothesis: nonsteroidal anti-inflammatory drugs reduce the incidence of large-bowel cancer. J. Natl. Cancer Inst. 83, 355 - 358." type="journal article" year="1991">Rosenberg et al., 1991</bibRefCitation>
;
<bibRefCitation id="EF974B35C666FFE5E86F4054FC41FDA6" author="Medzhitov, R." box="[818,979,574,593]" pageId="4" pageNumber="197" pagination="428 - 435" refId="ref10225" refString="Medzhitov, R., 2008. Origin and physiological roles of inflammation. Nature 454 (7203), 428 - 435." type="journal article" year="2008">Medzhitov, 2008</bibRefCitation>
). Hence, extracts and selected pure compounds from the bark of
<taxonomicName id="4C064D47C666FFE5E8FA4030FB82FD9A" box="[935,1040,602,621]" class="Magnoliopsida" family="Euphorbiaceae" genus="Croton" kingdom="Plantae" order="Malpighiales" pageId="4" pageNumber="197" phylum="Tracheophyta" rank="species" species="oblongus">
<emphasis id="B972EAD6C666FFE5E8FA4030FB82FD9A" bold="true" box="[935,1040,602,621]" italics="true" pageId="4" pageNumber="197">C. oblongus</emphasis>
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were evaluated for their cytotoxicity and the closely related anti-inflammatory activity via inhibition of LPS-induced NF-κB translocation in RAW 264.7 cells evaluation.
</paragraph>
<paragraph id="8BB936C4C666FFE5E80E40C4FA9FFC57" blockId="4.[818,1488,490,1123]" pageId="4" pageNumber="197">
Crude extracts of
<taxonomicName id="4C064D47C666FFE5E8A040C4FBF7FD36" box="[1021,1125,686,705]" class="Magnoliopsida" family="Euphorbiaceae" genus="Croton" kingdom="Plantae" order="Malpighiales" pageId="4" pageNumber="197" phylum="Tracheophyta" rank="species" species="oblongus">
<emphasis id="B972EAD6C666FFE5E8A040C4FBF7FD36" bold="true" box="[1021,1125,686,705]" italics="true" pageId="4" pageNumber="197">C. oblongus</emphasis>
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were screened against a panel of cell lines consisted of normal liver cell line (WRL-68), lung cancer cell line (A549), prostate cancer cell line (PC-3), melanoma cell line (A375), colon cancer cell line (HT-29) and human breast cancer cell lines (MCF-7). IC
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values of the hexane extract against these cell lines ranged between 35.0 and 66.1
<emphasis id="B972EAD6C666FFE5EF494153FB8CFCBB" box="[1044,1054,825,844]" italics="true" pageId="4" pageNumber="197">μ</emphasis>
g/mL while no activity recorded against HT-29 cell line. The
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Cl
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extract showed more selective activity, affecting only A549 (IC
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57.6
<emphasis id="B972EAD6C666FFE5EF17411BFBC6FC73" box="[1098,1108,881,900]" italics="true" pageId="4" pageNumber="197">μ</emphasis>
g/mL) and A375 (IC
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63.6
<emphasis id="B972EAD6C666FFE5EE3C411BFAF9FC73" box="[1377,1387,881,900]" italics="true" pageId="4" pageNumber="197">μ</emphasis>
g/mL) cell lines. The methanol extract did not show activity.
</paragraph>
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All purified compounds were isolated from hexane and
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Cl
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extracts, which in the initial screen had shown potential cytotoxicity. However, most compounds were found to have very weak activity with IC
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&gt; 100
<emphasis id="B972EAD6C666FFE5E8FB4196FC22FBF8" box="[934,944,1020,1039]" italics="true" pageId="4" pageNumber="197">μ</emphasis>
M. Only
<emphasis id="B972EAD6C666FFE5EF744196FBA6FBF8" box="[1065,1076,1020,1039]" italics="true" pageId="4" pageNumber="197">β</emphasis>
-amyrone and
<emphasis id="B972EAD6C666FFE5EFBF4196FB7FFBF8" box="[1250,1261,1020,1039]" italics="true" pageId="4" pageNumber="197">β</emphasis>
-sitostenone exhibited IC
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&lt;100
<emphasis id="B972EAD6C666FFE5E8FB4672FC22FBDC" box="[934,944,1048,1067]" italics="true" pageId="4" pageNumber="197">μ</emphasis>
M, 73 and 94
<emphasis id="B972EAD6C666FFE5EF6E4672FBAFFBDC" box="[1075,1085,1048,1067]" italics="true" pageId="4" pageNumber="197">μ</emphasis>
M respectively. Compounds
<emphasis id="B972EAD6C666FFE5EE144672FAC7FBDC" bold="true" box="[1353,1365,1048,1067]" pageId="4" pageNumber="197">1</emphasis>
,
<emphasis id="B972EAD6C666FFE5EE3F4672FAFCFBDC" bold="true" box="[1378,1390,1048,1067]" pageId="4" pageNumber="197">3</emphasis>
,
<emphasis id="B972EAD6C666FFE5EE274672FA17FBDC" box="[1402,1413,1048,1067]" italics="true" pageId="4" pageNumber="197">β</emphasis>
-amyrin and acetyl aleuritolic acid were also tested in the LPS-induced NF-κB translocation inhibition in RAW 264.7 cells assay. Compounds
<emphasis id="B972EAD6C666FFE5EEDB463AFA00FB94" bold="true" box="[1414,1426,1104,1123]" pageId="4" pageNumber="197">1</emphasis>
and
<emphasis id="B972EAD6C666FFE5EE9E463AFA5DFB94" bold="true" box="[1475,1487,1104,1123]" pageId="4" pageNumber="197">3</emphasis>
showed no activity while
<emphasis id="B972EAD6C667FFE4EA0042F5FEFAFF45" box="[349,360,159,178]" italics="true" pageId="5" pageNumber="198">β</emphasis>
-amyrin and acetyl aleuritolic acid showed good anti-inflammatory activity with IC
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values of 23.5 and 35.4
<emphasis id="B972EAD6C667FFE4E9BF42D1FD7EFF39" box="[738,748,187,206]" italics="true" pageId="5" pageNumber="198">μ</emphasis>
g/ mL respectively.
</paragraph>
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