277 lines
31 KiB
XML
277 lines
31 KiB
XML
<document id="51986834C71D195DC37FC26A636A1F24" ID-DOI="10.11646/phytotaxa.653.1.2" ID-ISSN="1179-3163" ID-Zenodo-Dep="13216863" IM.bibliography_approvedBy="carolina" IM.illustrations_approvedBy="carolina" IM.materialsCitations_approvedBy="carolina" IM.tables_approvedBy="carolina" IM.taxonomicNames_approvedBy="carolina" IM.treatments_approvedBy="carolina" checkinTime="1722603289460" checkinUser="felipe" docAuthor="Gong, Cui-Ling, Wu, Hai-Xia, Li, Jin-Chen & Song, Jia-Yu" docDate="2024" docId="03BE879BFFB3C81ED4A1FC35FAD75493" docLanguage="en" docName="phytotaxa.653.1.2.pdf" docOrigin="Phytotaxa 653 (1)" docSource="http://dx.doi.org/10.11646/phytotaxa.653.1.2" docStyle="DocumentStyle:96748F8F1B6C902996E134952A3A36B9.13:Phytotaxa.2014-.journal_article" docStyleId="96748F8F1B6C902996E134952A3A36B9" docStyleName="Phytotaxa.2014-.journal_article" docStyleVersion="13" docTitle="Phyllosticta ovalina H. X. Wu & C. L. Gong., sp. nov." docType="treatment" docVersion="3" lastPageNumber="29" masterDocId="FF87FFE3FFBAC817D429FFEEFF8F5375" masterDocTitle="Morphological and multi-gene phylogenetic analyses of two new species of Phyllosticta (Phyllostictaceae, Botryosphaeriales) from Southwestern China" masterLastPageNumber="36" masterPageNumber="20" pageNumber="29" updateTime="1724884690337" updateUser="carolina" zenodo-license-figures="UNSPECIFIED" zenodo-license-treatments="UNSPECIFIED">
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<mods:title id="A504CA3010C8D7A6DFD19C43D7C5D044">Morphological and multi-gene phylogenetic analyses of two new species of Phyllosticta (Phyllostictaceae, Botryosphaeriales) from Southwestern China</mods:title>
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<mods:namePart id="8F907D4E95546DFCCD9557A0E1F90218">Gong, Cui-Ling</mods:namePart>
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<mods:affiliation id="9D10F424BFB3520625EDBF4AF8F88BAF">International Fungal Research and Development Centre, Institute of Highland Forest Science, Chinese Academy of Forestry, Kunming 650224, Yunnan, P. R. of China. & Nanjing Forestry University, Nanjing 210037, P. R. of China.</mods:affiliation>
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<mods:nameIdentifier id="AFCADA84E93C0496178114FFCA5A7759" type="email">gcl020829@163.com</mods:nameIdentifier>
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<mods:roleTerm id="CA84B7CE2F6912C7F25D4BD3DFA8C9C9">Author</mods:roleTerm>
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<mods:namePart id="704F046DBAEBA020F508576AFD84BF41">Wu, Hai-Xia</mods:namePart>
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<mods:affiliation id="A8D2B1132FFE4AC6EEA052D85C474F63">International Fungal Research and Development Centre, Institute of Highland Forest Science, Chinese Academy of Forestry, Kunming 650224, Yunnan, P. R. of China.</mods:affiliation>
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<mods:nameIdentifier id="BC97B15427923FBE345F78977E7E2ED1" type="email">aileen2008haixia@gmail.com</mods:nameIdentifier>
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<mods:namePart id="695C90A94265C73491B29740AE9BBFC9">Li, Jin-Chen</mods:namePart>
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<mods:affiliation id="D7EA6AA6681E2E785D26B82849860CAE">International Fungal Research and Development Centre, Institute of Highland Forest Science, Chinese Academy of Forestry, Kunming 650224, Yunnan, P. R. of China.</mods:affiliation>
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<mods:nameIdentifier id="F086C84B214904CA498F6891E5F88B2F" type="email">jinchenlii@163.com</mods:nameIdentifier>
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<mods:namePart id="11E8C37AFBE70136BB5DDCC6187A8545">Song, Jia-Yu</mods:namePart>
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<mods:affiliation id="8F0AE89357F2CB572FA174D2F6979148">International Fungal Research and Development Centre, Institute of Highland Forest Science, Chinese Academy of Forestry, Kunming 650224, Yunnan, P. R. of China.</mods:affiliation>
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<mods:nameIdentifier id="142DEE269492BC968C45A50A0296A1B0" type="email">ldsat-f@outlook.com</mods:nameIdentifier>
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<mods:title id="7875728D2FBE36A8375504140A8E39B7">Phytotaxa</mods:title>
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<mods:part id="4B72A7A0BDF70CD599C5BC1783F30E9F">
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<mods:date id="42EA14B3EDF1039C5ED98E77572EA1B6">2024</mods:date>
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<mods:number id="3D27C520AC0A3F6078879797D08E0275">2024-06-13</mods:number>
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<mods:number id="863E215A4C771382055F2721FFEDB388">653</mods:number>
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<mods:start id="ED6BC32CC0A976D9DD6A7B0B3B42B553">20</mods:start>
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<mods:url id="39A72988C62522EF54A47D0755962976">http://dx.doi.org/10.11646/phytotaxa.653.1.2</mods:url>
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<mods:classification id="4E0A54FA4F2D3EE1331253D02985C680">journal article</mods:classification>
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<mods:identifier id="6F126B7C6AA6E3BA37E769CDA43D5105" type="DOI">10.11646/phytotaxa.653.1.2</mods:identifier>
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<mods:identifier id="532E9044C42F7F579892C9966C07FD1C" type="ISSN">1179-3163</mods:identifier>
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<subSubSection id="C30D6506FFB3C81ED4A1FC35FC905080" box="[136,799,987,1014]" pageId="9" pageNumber="29" type="nomenclature">
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<paragraph id="8BA8368DFFB3C81ED4A1FC35FC905080" blockId="9.[136,799,987,1014]" box="[136,799,987,1014]" pageId="9" pageNumber="29">
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<heading id="D0E081E1FFB3C81ED4A1FC35FC905080" box="[136,799,987,1014]" fontSize="11" level="2" pageId="9" pageNumber="29" reason="3">
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<taxonomicName id="4C174D0EFFB3C81ED4A1FC35FDF55080" authority="H.X. Wu & C.L. Gong." authorityName="H. X. Wu & C. L. Gong." box="[136,634,987,1014]" class="Sordariomycetes" family="Diaporthaceae" genus="Phyllosticta" kingdom="Fungi" order="Diaporthales" pageId="9" pageNumber="29" phylum="Ascomycota" rank="species" species="ovalina" status="sp. nov.">
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<emphasis id="B963EA9FFFB3C81ED4A1FC35FEE35083" bold="true" box="[136,364,987,1014]" italics="true" pageId="9" pageNumber="29">Phyllosticta ovalina</emphasis>
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H.X. Wu & C.L. Gong.
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</taxonomicName>
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<taxonomicNameLabel id="A25057E4FFB3C81ED6A9FC32FD595083" box="[640,726,988,1014]" pageId="9" pageNumber="29" rank="species">
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<emphasis id="B963EA9FFFB3C81ED6A9FC32FD405080" box="[640,719,988,1013]" italics="true" pageId="9" pageNumber="29">sp. nov</emphasis>
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.
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</taxonomicNameLabel>
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<figureCitation id="132C2A08FFB3C81ED6F4FC35FC905080" box="[733,799,987,1013]" captionStart="FIGURE 3" captionStartId="12.[136,229,1142,1164]" captionTargetBox="[412,1177,191,1117]" captionTargetId="figure-18@12.[410,1178,190,1118]" captionTargetPageId="12" captionText="FIGURE 3. Phyllosticta ovalina (IFRD 9478, holotype). a. Microfungi on leaf litter. b–c. Ascomata on leaf surface. d–e. Vertical section of ascomata. f–i. Asci. j–n. Ascospores (i in cotton blue). Scale bars: b = 500 μm, c = 100 μm, d = 50 μm, f–i = 20 μm, j–n = 10 μm." figureDoi="http://doi.org/10.5281/zenodo.13216869" httpUri="https://zenodo.org/record/13216869/files/figure.png" pageId="9" pageNumber="29">Fig. 3</figureCitation>
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</heading>
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</paragraph>
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<subSubSection id="C30D6506FFB3C81ED4A1FBEDFE02576C" box="[136,397,1027,1049]" pageId="9" pageNumber="29" type="description">
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<paragraph id="8BA8368DFFB3C81ED4A1FBEDFE02576C" blockId="9.[136,753,1027,1120]" box="[136,397,1027,1049]" pageId="9" pageNumber="29">Fungal Names: FN 571912</paragraph>
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</subSubSection>
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<subSubSection id="C30D6506FFB3C81ED4A1FBC9FD7E5749" box="[136,753,1063,1085]" pageId="9" pageNumber="29" type="etymology">
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<paragraph id="8BA8368DFFB3C81ED4A1FBC9FD7E5749" blockId="9.[136,753,1027,1120]" box="[136,753,1063,1085]" pageId="9" pageNumber="29">
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<emphasis id="B963EA9FFFB3C81ED4A1FBC9FE815749" bold="true" box="[136,270,1063,1084]" pageId="9" pageNumber="29">Etymology—</emphasis>
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<taxonomicName id="4C174D0EFFB3C81ED526FBC9FED85749" box="[271,343,1063,1084]" class="Sordariomycetes" family="Diaporthaceae" genus="Phyllosticta" kingdom="Fungi" order="Diaporthales" pageId="9" pageNumber="29" phylum="Ascomycota" rank="species" species="ovalina">
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<emphasis id="B963EA9FFFB3C81ED526FBC9FED85749" box="[271,343,1063,1084]" italics="true" pageId="9" pageNumber="29">ovalina</emphasis>
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</taxonomicName>
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= oval, in reference to its oval ascospores.
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</paragraph>
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</subSubSection>
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<subSubSection id="C30D6506FFB3C81ED4A1FBA5FEE55715" box="[136,362,1099,1120]" pageId="9" pageNumber="29" type="materials_examined">
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||
<paragraph id="8BA8368DFFB3C81ED4A1FBA5FEE55715" blockId="9.[136,753,1027,1120]" box="[136,362,1099,1120]" pageId="9" pageNumber="29">
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<materialsCitation id="3B7F3CD0FFB3C81ED4A1FBA5FEE55715" box="[136,362,1099,1120]" collectionCode="IFRD" pageId="9" pageNumber="29" specimenCode="IFRD 9478" specimenCount="1" typeStatus="holotype">
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<emphasis id="B963EA9FFFB3C81ED4A1FBA5FF725715" bold="true" box="[136,253,1099,1120]" pageId="9" pageNumber="29">
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<typeStatus id="54AC882FFFB3C81ED4A1FBA5FF6A5715" box="[136,229,1099,1120]" pageId="9" pageNumber="29" type="holotype">Holotype</typeStatus>
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—
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</emphasis>
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<specimenCode id="DBB19EF6FFB3C81ED4D4FBA5FEE55715" box="[253,362,1099,1120]" collectionCode="IFRD" country="China" httpUri="http://grbio.org/cool/dj2g-9ene" name="Research Institute of Resource Insects" pageId="9" pageNumber="29" type="Herbarium">IFRD 9478</specimenCode>
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</materialsCitation>
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</paragraph>
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||
</subSubSection>
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<subSubSection id="C30D6506FFB3C81ED4A1FB61FAD756D0" pageId="9" pageNumber="29" type="description">
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<paragraph id="8BA8368DFFB3C81ED4A1FB61FAD756D0" blockId="9.[136,1452,1167,2022]" pageId="9" pageNumber="29">
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Saprobic on leaf litter.
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<emphasis id="B963EA9FFFB3C81ED5ABFB61FDA757DC" bold="true" box="[386,552,1167,1193]" pageId="9" pageNumber="29">Sexual morph</emphasis>
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: Black ascomata observed on leaf surface.Ascomata125–215 μm (
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<emphasis id="B963EA9FFFB3C81ED126FB7DFA9357D6" box="[1295,1308,1171,1187]" italics="true" pageId="9" pageNumber="29">x</emphasis>
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= 164 μm, n = 20), semi-buried, globose, black, carbonaceous, uneven surface, raised centre, irregularly rounded, domed above the leaf surface, releasing ascospores at maturity by dehiscence and forming a central ostiole. Peridium thin, composed of brown to dark brown cells of
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<emphasis id="B963EA9FFFB3C81ED5EEFB12FD0A5660" box="[455,645,1275,1301]" italics="true" pageId="9" pageNumber="29">textura angularis</emphasis>
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. Asci 45–110 × 10–20 μm (
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<emphasis id="B963EA9FFFB3C81ED793FB11FC48567A" box="[954,967,1279,1295]" italics="true" pageId="9" pageNumber="29">x</emphasis>
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= 68 × 16 μm, n= 20), 8-spored, bitunicate, clavate to broadly ellipsoid, with a broad, gradually tapering, obtuse apex, with an apical ring and a chamber diameter of 2.2 μm. Ascospores 15–20 × 5–10 μm (
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<emphasis id="B963EA9FFFB3C81ED674FAA9FDE55622" box="[605,618,1351,1367]" italics="true" pageId="9" pageNumber="29">x</emphasis>
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= 17 × 8 um, n= 20), biseriate, ovoid to ellipsoid, obtusely rounded at both ends, widest at the apex and slightly narrower at the base, with a large central guttle, a few ascospores with slightly appendage at the apex or base, and generally without appendage at both ends.
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<emphasis id="B963EA9FFFB3C81ED7DDFA65FB2456D0" bold="true" box="[1012,1195,1419,1445]" pageId="9" pageNumber="29">Asexual morph</emphasis>
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: Not observed.
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</paragraph>
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</subSubSection>
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<subSubSection id="C30D6506FFB3C81ED494FA41FDE65564" pageId="9" pageNumber="29" type="materials_examined">
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<paragraph id="8BA8368DFFB3C81ED494FA41FDE65564" blockId="9.[136,1452,1167,2022]" pageId="9" pageNumber="29">
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<emphasis id="B963EA9FFFB3C81ED494FA41FE3056BC" bold="true" box="[189,447,1455,1481]" pageId="9" pageNumber="29">Material examined—</emphasis>
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<collectingCountry id="F300761DFFB3C81ED596FA41FD9456BC" box="[447,539,1455,1481]" name="China" pageId="9" pageNumber="29">CHINA</collectingCountry>
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.
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<collectingRegion id="49D3F86FFFB3C81ED60EFA5EFD6056BC" box="[551,751,1455,1482]" country="China" name="Yunnan" pageId="9" pageNumber="29">Yunnan Province</collectingRegion>
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: MouDing County, HuaFoShan National Nature Reserve, on dead leaves of an unidentified plant,
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<geoCoordinate id="EE23504AFFB3C81ED606FA3DFD04569B" box="[559,651,1491,1518]" degrees="25" direction="north" minutes="18" orientation="latitude" pageId="9" pageNumber="29" precision="925" value="25.3">25°18'N</geoCoordinate>
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–
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<geoCoordinate id="EE23504AFFB3C81ED6B2FA3DFD77569B" box="[667,760,1491,1518]" degrees="25" direction="north" minutes="31" orientation="latitude" pageId="9" pageNumber="29" precision="925" value="25.516666">25°31'N</geoCoordinate>
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,
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<geoCoordinate id="EE23504AFFB3C81ED72CFA3DFCE3569B" box="[773,876,1491,1518]" degrees="101" direction="east" minutes="25" orientation="longitude" pageId="9" pageNumber="29" precision="925" value="101.416664">101°25'E</geoCoordinate>
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–
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||
<geoCoordinate id="EE23504AFFB3C81ED755FA3DFC6B569B" box="[892,996,1491,1518]" degrees="101" direction="east" minutes="42" orientation="longitude" pageId="9" pageNumber="29" precision="925" value="101.7">101°42'E</geoCoordinate>
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,
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<quantity id="4CEF9B68FFB3C81ED7DBFA3DFBC7569B" box="[1010,1096,1491,1518]" metricMagnitude="3" metricUnit="m" metricValue="2.36" pageId="9" pageNumber="29" unit="m" value="2360.0">2360 m</quantity>
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elev.,
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<date id="FFA9104DFFB3C81ED0BDFA3DFABD5698" box="[1172,1330,1491,1517]" pageId="9" pageNumber="29" value="2021-10">October 2021</date>
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, H.X. Wu, J.C. L & J.Y. Song (
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<collectionCode id="ED06AE48FFB3C81ED54EFA19FE265564" box="[359,425,1527,1553]" country="China" httpUri="http://grbio.org/cool/dj2g-9ene" name="Research Institute of Resource Insects" pageId="9" pageNumber="29" type="Herbarium">IFRD</collectionCode>
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9478,
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<typeStatus id="54AC882FFFB3C81ED5DFFA19FDD25564" box="[502,605,1527,1553]" pageId="9" pageNumber="29" type="holotype">holotype</typeStatus>
|
||
).
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</paragraph>
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</subSubSection>
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<subSubSection id="C30D6506FFB3C81ED494F9F5FAD75493" pageId="9" pageNumber="29" type="discussion">
|
||
<paragraph id="8BA8368DFFB3C81ED494F9F5FA0655B0" blockId="9.[136,1452,1167,2022]" pageId="9" pageNumber="29">
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<emphasis id="B963EA9FFFB3C81ED494F9F5FE8F5540" bold="true" box="[189,256,1563,1589]" pageId="9" pageNumber="29">Notes</emphasis>
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: In the phylogenetic tree
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<taxonomicName id="4C174D0EFFB3C81ED636F9F2FD005540" box="[543,655,1563,1589]" class="Sordariomycetes" family="Diaporthaceae" genus="Phyllosticta" kingdom="Fungi" order="Diaporthales" pageId="9" pageNumber="29" phylum="Ascomycota" rank="species" species="ovalina">
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<emphasis id="B963EA9FFFB3C81ED636F9F2FD005540" box="[543,655,1563,1589]" italics="true" pageId="9" pageNumber="29">P. ovalina</emphasis>
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</taxonomicName>
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is placed in the
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<taxonomicName id="4C174D0EFFB3C81ED76FF9F2FC385540" box="[838,951,1564,1589]" class="Sordariomycetes" family="Diaporthaceae" genus="Phyllosticta" kingdom="Fungi" order="Diaporthales" pageId="9" pageNumber="29" phylum="Ascomycota" rank="species" species="cruenta">
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<emphasis id="B963EA9FFFB3C81ED76FF9F2FC385540" box="[838,951,1564,1589]" italics="true" pageId="9" pageNumber="29">P. cruenta</emphasis>
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</taxonomicName>
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species complex (
|
||
<figureCitation id="132C2A08FFB3C81ED0AFF9F5FB485540" box="[1158,1223,1563,1589]" captionStart="FIGURE 1" captionStartId="3.[136,229,1824,1846]" captionTargetBox="[151,1435,422,1796]" captionTargetId="figure-150@3.[151,1435,422,1796]" captionTargetPageId="3" captionText="FIGURE 1. Phylogram of Phyllosticta resulting from a maximum likelihood analysis based on a combined matrix of ITS, LSU and TEF1 loci. The tree is artificially rooted to B. obtusa (CMW 8232). ML bootstrap values (left, ML-BS ≥ 50%) and Bayesian posterior probabilities (right, BYPP ≥ 0.9) are given at the nodes. New species of this study are indicated using red font and their associated support is bolded." figureDoi="http://doi.org/10.5281/zenodo.13216865" httpUri="https://zenodo.org/record/13216865/files/figure.png" pageId="9" pageNumber="29">Fig. 1</figureCitation>
|
||
), which contains 21 species (
|
||
<tableCitation id="C6950336FFB3C81ED4CEF9D1FEB5552C" box="[231,314,1599,1625]" captionStart="TABLE 1" captionStartId="6.[136,228,158,182]" captionTargetPageId="6" captionText="TABLE 1. A List of species, GenBank accession numbers of the sequences used for phylogenetic analysis. Sequences" pageId="9" pageNumber="29">Table 1</tableCitation>
|
||
), with only two sexual stage species,
|
||
<taxonomicName id="4C174D0EFFB3C81ED6F7F9AEFCE6552C" box="[734,873,1599,1625]" class="Sordariomycetes" family="Diaporthaceae" genus="Phyllosticta" kingdom="Fungi" order="Diaporthales" pageId="9" pageNumber="29" phylum="Ascomycota" rank="species" species="abieticola">
|
||
<emphasis id="B963EA9FFFB3C81ED6F7F9AEFCE6552C" box="[734,873,1599,1625]" italics="true" pageId="9" pageNumber="29">P. abieticola</emphasis>
|
||
</taxonomicName>
|
||
, and
|
||
<taxonomicName id="4C174D0EFFB3C81ED78CF9AEFB9E552C" box="[933,1041,1599,1625]" class="Sordariomycetes" family="Diaporthaceae" genus="Phyllosticta" kingdom="Fungi" order="Diaporthales" pageId="9" pageNumber="29" phylum="Ascomycota" rank="species" species="rubella">
|
||
<emphasis id="B963EA9FFFB3C81ED78CF9AEFB9E552C" box="[933,1041,1599,1625]" italics="true" pageId="9" pageNumber="29">P. rubella</emphasis>
|
||
</taxonomicName>
|
||
being reported (
|
||
<bibRefCitation id="EF864B7CFFB3C81ED0EEF9D1FA2F552C" author="Wikee, S. & Lombard, L. & Nakashima, C. & Motohashi, K. & Chukeatirote, E. & Cheewangkoon, R. & McKenzie, E. H. C. & Hyde, K. D. & Crous, P. W." box="[1223,1440,1599,1625]" pageId="9" pageNumber="29" pagination="1 - 29" refId="ref11668" refString="Wikee, S., Lombard, L., Nakashima, C., Motohashi, K., Chukeatirote, E., Cheewangkoon, R., McKenzie, E. H. C., Hyde, K. D. & Crous, P. W. (2013 b) A phylogenetic re-evaluation of Phyllosticta (Botryosphaeriales). Studies in Mycology 76 (1): 1 - 29. https: // doi. org / 10.3114 / sim 0019" type="journal article" year="2013">
|
||
Wikee
|
||
<emphasis id="B963EA9FFFB3C81ED13EF9AEFAC0552C" box="[1303,1359,1599,1625]" italics="true" pageId="9" pageNumber="29">et al.</emphasis>
|
||
2013b
|
||
</bibRefCitation>
|
||
).
|
||
<taxonomicName id="4C174D0EFFB3C81ED4A1F98AFF735508" box="[136,252,1635,1661]" class="Sordariomycetes" family="Diaporthaceae" genus="Phyllosticta" kingdom="Fungi" order="Diaporthales" pageId="9" pageNumber="29" phylum="Ascomycota" rank="species" species="ovalina">
|
||
<emphasis id="B963EA9FFFB3C81ED4A1F98AFF735508" box="[136,252,1635,1661]" italics="true" pageId="9" pageNumber="29">P. ovalina</emphasis>
|
||
</taxonomicName>
|
||
differs significantly from these two sexual stage species in morphological characters. The ascospores of
|
||
<taxonomicName id="4C174D0EFFB3C81ED480F966FEB855D4" box="[169,311,1671,1697]" class="Sordariomycetes" family="Diaporthaceae" genus="Phyllosticta" kingdom="Fungi" order="Diaporthales" pageId="9" pageNumber="29" phylum="Ascomycota" rank="species" species="abieticola">
|
||
<emphasis id="B963EA9FFFB3C81ED480F966FEB855D4" box="[169,311,1671,1697]" italics="true" pageId="9" pageNumber="29">P. abieticola</emphasis>
|
||
</taxonomicName>
|
||
are multiguttulate, widest at the centre, limoniform with obtuse ends. Ascospores of
|
||
<taxonomicName id="4C174D0EFFB3C81ED138F966FA0F55D4" box="[1297,1408,1671,1697]" class="Sordariomycetes" family="Diaporthaceae" genus="Phyllosticta" kingdom="Fungi" order="Diaporthales" pageId="9" pageNumber="29" phylum="Ascomycota" rank="species" species="rubella">
|
||
<emphasis id="B963EA9FFFB3C81ED138F966FA0F55D4" box="[1297,1408,1671,1697]" italics="true" pageId="9" pageNumber="29">P. rubella</emphasis>
|
||
</taxonomicName>
|
||
are limoniform, widest in the upper third, whereas ascospores of
|
||
<taxonomicName id="4C174D0EFFB3C81ED71EF942FC2955B0" box="[823,934,1707,1733]" class="Sordariomycetes" family="Diaporthaceae" genus="Phyllosticta" kingdom="Fungi" order="Diaporthales" pageId="9" pageNumber="29" phylum="Ascomycota" rank="species" species="ovalina">
|
||
<emphasis id="B963EA9FFFB3C81ED71EF942FC2955B0" box="[823,934,1707,1733]" italics="true" pageId="9" pageNumber="29">P. ovalina</emphasis>
|
||
</taxonomicName>
|
||
are ovoid and widest at the apex (
|
||
<tableCitation id="C6950336FFB3C81ED10DF945FAF355B0" box="[1316,1404,1707,1733]" captionStart="TABLE 2" captionStartId="10.[136,224,159,183]" captionTargetPageId="10" captionText="TABLE 2. Morphological characteristics comparison of Phyllosticta species related to this study. L to W = length/width ratio" pageId="9" pageNumber="29">Table 2</tableCitation>
|
||
).
|
||
</paragraph>
|
||
<paragraph id="8BA8368DFFB3C81ED494F921FAD75493" blockId="9.[136,1452,1167,2022]" pageId="9" pageNumber="29">
|
||
The morphological distinctions were further corroborated by the phylogenetic analysis, indicating that
|
||
<taxonomicName id="4C174D0EFFB3C81ED115F93EFA24559C" box="[1340,1451,1743,1769]" class="Sordariomycetes" family="Diaporthaceae" genus="Phyllosticta" kingdom="Fungi" order="Diaporthales" pageId="9" pageNumber="29" phylum="Ascomycota" rank="species" species="ovalina">
|
||
<emphasis id="B963EA9FFFB3C81ED115F93EFA24559C" box="[1340,1451,1743,1769]" italics="true" pageId="9" pageNumber="29">P. ovalina</emphasis>
|
||
</taxonomicName>
|
||
does not cluster with these two sexual species (
|
||
<figureCitation id="132C2A08FFB3C81ED6B0F91DFD555478" box="[665,730,1779,1805]" captionStart="FIGURE 1" captionStartId="3.[136,229,1824,1846]" captionTargetBox="[151,1435,422,1796]" captionTargetId="figure-150@3.[151,1435,422,1796]" captionTargetPageId="3" captionText="FIGURE 1. Phylogram of Phyllosticta resulting from a maximum likelihood analysis based on a combined matrix of ITS, LSU and TEF1 loci. The tree is artificially rooted to B. obtusa (CMW 8232). ML bootstrap values (left, ML-BS ≥ 50%) and Bayesian posterior probabilities (right, BYPP ≥ 0.9) are given at the nodes. New species of this study are indicated using red font and their associated support is bolded." figureDoi="http://doi.org/10.5281/zenodo.13216865" httpUri="https://zenodo.org/record/13216865/files/figure.png" pageId="9" pageNumber="29">Fig. 1</figureCitation>
|
||
). The phylogenetic analyses reveal a close relationship between
|
||
<taxonomicName id="4C174D0EFFB3C81ED4A1F8F6FF795444" box="[136,246,1815,1841]" class="Sordariomycetes" family="Diaporthaceae" genus="Phyllosticta" kingdom="Fungi" order="Diaporthales" pageId="9" pageNumber="29" phylum="Ascomycota" rank="species" species="ovalina">
|
||
<emphasis id="B963EA9FFFB3C81ED4A1F8F6FF795444" box="[136,246,1815,1841]" italics="true" pageId="9" pageNumber="29">P. ovalina</emphasis>
|
||
</taxonomicName>
|
||
and
|
||
<taxonomicName id="4C174D0EFFB3C81ED503F8F6FE355444" box="[298,442,1815,1841]" class="Sordariomycetes" family="Diaporthaceae" genus="Phyllosticta" kingdom="Fungi" order="Diaporthales" pageId="9" pageNumber="29" phylum="Ascomycota" rank="species" species="hubeiensis">
|
||
<emphasis id="B963EA9FFFB3C81ED503F8F6FE355444" box="[298,442,1815,1841]" italics="true" pageId="9" pageNumber="29">P. hubeiensis</emphasis>
|
||
</taxonomicName>
|
||
(
|
||
<figureCitation id="132C2A08FFB3C81ED5E1F8F9FD875444" box="[456,520,1815,1841]" captionStart="FIGURE 1" captionStartId="3.[136,229,1824,1846]" captionTargetBox="[151,1435,422,1796]" captionTargetId="figure-150@3.[151,1435,422,1796]" captionTargetPageId="3" captionText="FIGURE 1. Phylogram of Phyllosticta resulting from a maximum likelihood analysis based on a combined matrix of ITS, LSU and TEF1 loci. The tree is artificially rooted to B. obtusa (CMW 8232). ML bootstrap values (left, ML-BS ≥ 50%) and Bayesian posterior probabilities (right, BYPP ≥ 0.9) are given at the nodes. New species of this study are indicated using red font and their associated support is bolded." figureDoi="http://doi.org/10.5281/zenodo.13216865" httpUri="https://zenodo.org/record/13216865/files/figure.png" pageId="9" pageNumber="29">Fig. 1</figureCitation>
|
||
), yet
|
||
<taxonomicName id="4C174D0EFFB3C81ED66EF8F6FD3A5444" box="[583,693,1815,1841]" class="Sordariomycetes" family="Diaporthaceae" genus="Phyllosticta" kingdom="Fungi" order="Diaporthales" pageId="9" pageNumber="29" phylum="Ascomycota" rank="species" species="ovalina">
|
||
<emphasis id="B963EA9FFFB3C81ED66EF8F6FD3A5444" box="[583,693,1815,1841]" italics="true" pageId="9" pageNumber="29">P. ovalina</emphasis>
|
||
</taxonomicName>
|
||
forms a distinct branch with strong support (100%/1.00, BS/
|
||
<collectionCode id="ED06AE48FFB3C81ED17CF8F9FA2F5444" box="[1365,1440,1815,1841]" pageId="9" pageNumber="29">BYPP</collectionCode>
|
||
). Furthermore,
|
||
<collectionCode id="ED06AE48FFB3C81ED50DF8D5FEE85420" box="[292,359,1851,1877]" pageId="9" pageNumber="29">NCBI</collectionCode>
|
||
blast results of the
|
||
<collectionCode id="ED06AE48FFB3C81ED663F8D5FDFB5420" box="[586,628,1851,1877]" pageId="9" pageNumber="29">ITS</collectionCode>
|
||
sequences of
|
||
<taxonomicName id="4C174D0EFFB3C81ED73EF8D2FC075420" box="[791,904,1851,1877]" class="Sordariomycetes" family="Diaporthaceae" genus="Phyllosticta" kingdom="Fungi" order="Diaporthales" pageId="9" pageNumber="29" phylum="Ascomycota" rank="species" species="ovalina">
|
||
<emphasis id="B963EA9FFFB3C81ED73EF8D2FC075420" box="[791,904,1851,1877]" italics="true" pageId="9" pageNumber="29">P. ovalina</emphasis>
|
||
</taxonomicName>
|
||
(
|
||
<typeStatus id="54AC882FFFB3C81ED7B1F8D5FC725420" box="[920,1021,1851,1877]" pageId="9" pageNumber="29" type="holotype">holotype</typeStatus>
|
||
) also support our findings.
|
||
<taxonomicName id="4C174D0EFFB3C81ED113F8D2FA245420" box="[1338,1451,1851,1877]" class="Sordariomycetes" family="Diaporthaceae" genus="Phyllosticta" kingdom="Fungi" order="Diaporthales" pageId="9" pageNumber="29" phylum="Ascomycota" rank="species" species="ovalina">
|
||
<emphasis id="B963EA9FFFB3C81ED113F8D2FA245420" box="[1338,1451,1851,1877]" italics="true" pageId="9" pageNumber="29">P. ovalina</emphasis>
|
||
</taxonomicName>
|
||
exhibits only 95.37% sequence identity with
|
||
<taxonomicName id="4C174D0EFFB3C81ED6A2F88EFC91540C" box="[651,798,1887,1913]" class="Sordariomycetes" family="Diaporthaceae" genus="Phyllosticta" kingdom="Fungi" order="Diaporthales" pageId="9" pageNumber="29" phylum="Ascomycota" rank="species" species="hubeiensis">
|
||
<emphasis id="B963EA9FFFB3C81ED6A2F88EFC91540C" box="[651,798,1887,1913]" italics="true" pageId="9" pageNumber="29">P. hubeiensis</emphasis>
|
||
</taxonomicName>
|
||
(query cover = 35%), which does not align with it being a sexual stage of
|
||
<taxonomicName id="4C174D0EFFB3C81ED566F86AFE6D54E8" box="[335,482,1923,1949]" class="Sordariomycetes" family="Diaporthaceae" genus="Phyllosticta" kingdom="Fungi" order="Diaporthales" pageId="9" pageNumber="29" phylum="Ascomycota" rank="species" species="hubeiensis">
|
||
<emphasis id="B963EA9FFFB3C81ED566F86AFE6D54E8" box="[335,482,1923,1949]" italics="true" pageId="9" pageNumber="29">P. hubeiensis</emphasis>
|
||
</taxonomicName>
|
||
. Both
|
||
<taxonomicName id="4C174D0EFFB3C81ED618F86AFD2D54E8" box="[561,674,1923,1949]" class="Sordariomycetes" family="Diaporthaceae" genus="Phyllosticta" kingdom="Fungi" order="Diaporthales" pageId="9" pageNumber="29" phylum="Ascomycota" rank="species" species="ovalina">
|
||
<emphasis id="B963EA9FFFB3C81ED618F86AFD2D54E8" box="[561,674,1923,1949]" italics="true" pageId="9" pageNumber="29">P. ovalina</emphasis>
|
||
</taxonomicName>
|
||
and
|
||
<taxonomicName id="4C174D0EFFB3C81ED6F5F86AFCE654E8" box="[732,873,1923,1949]" class="Sordariomycetes" family="Diaporthaceae" genus="Phyllosticta" kingdom="Fungi" order="Diaporthales" pageId="9" pageNumber="29" phylum="Ascomycota" rank="species" species="abieticola">
|
||
<emphasis id="B963EA9FFFB3C81ED6F5F86AFCE654E8" box="[732,873,1923,1949]" italics="true" pageId="9" pageNumber="29">P. abieticola</emphasis>
|
||
</taxonomicName>
|
||
share only 85.10% sequence identity (query cover = 36%), while
|
||
<taxonomicName id="4C174D0EFFB3C81ED518F846FE2254B4" box="[305,429,1959,1985]" class="Sordariomycetes" family="Diaporthaceae" genus="Phyllosticta" kingdom="Fungi" order="Diaporthales" pageId="9" pageNumber="29" phylum="Ascomycota" rank="species" species="foliorum">
|
||
<emphasis id="B963EA9FFFB3C81ED518F846FE2254B4" box="[305,429,1959,1985]" italics="true" pageId="9" pageNumber="29">P. foliorum</emphasis>
|
||
</taxonomicName>
|
||
demonstrates only 86.29% (query cover = 36%) and 85.95% (query cover = 36%) with
|
||
<taxonomicName id="4C174D0EFFB3C81ED1BEF846FEA45490" class="Sordariomycetes" family="Diaporthaceae" genus="Phyllosticta" kingdom="Fungi" order="Diaporthales" pageId="9" pageNumber="29" phylum="Ascomycota" rank="species" species="podocarpicola">
|
||
<emphasis id="B963EA9FFFB3C81ED1BEF846FEA45490" italics="true" pageId="9" pageNumber="29">P. podocarpicola</emphasis>
|
||
</taxonomicName>
|
||
(
|
||
<tableCitation id="C6950336FFB3C81ED513F825FE1F5490" box="[314,400,1995,2021]" captionStart="TABLE 3" captionStartId="11.[136,216,1024,1045]" captionTargetPageId="11" captionText="TABLE 3. GenBank BLAST search results of Phyllosticta that clustered with P. savannaensis and P. ovalina." pageId="9" pageNumber="29">Table 3</tableCitation>
|
||
). Consequently,
|
||
<taxonomicName id="4C174D0EFFB3C81ED665F822FD345490" box="[588,699,1995,2021]" class="Sordariomycetes" family="Diaporthaceae" genus="Phyllosticta" kingdom="Fungi" order="Diaporthales" pageId="9" pageNumber="29" phylum="Ascomycota" rank="species" species="ovalina">
|
||
<emphasis id="B963EA9FFFB3C81ED665F822FD345490" box="[588,699,1995,2021]" italics="true" pageId="9" pageNumber="29">P. ovalina</emphasis>
|
||
</taxonomicName>
|
||
is classified as a new species within the genus
|
||
<taxonomicName id="4C174D0EFFB3C81ED0E2F825FADE5490" box="[1227,1361,1995,2021]" class="Sordariomycetes" family="Diaporthaceae" genus="Phyllosticta" kingdom="Fungi" order="Diaporthales" pageId="9" pageNumber="29" phylum="Ascomycota" rank="genus">
|
||
<emphasis id="B963EA9FFFB3C81ED0E2F825FADE5490" box="[1227,1361,1995,2021]" italics="true" pageId="9" pageNumber="29">Phyllosticta</emphasis>
|
||
</taxonomicName>
|
||
.
|
||
</paragraph>
|
||
</subSubSection>
|
||
</treatment>
|
||
</document> |