620 lines
134 KiB
XML
620 lines
134 KiB
XML
<document id="C3DE18D70C3CCF441FEF9232A094AF56" ID-DOI="10.4467/16890027AP.15.014.3211" ID-ISSN="1689-0027" ID-Zenodo-Dep="12638196" IM.bibliography_approvedBy="felipe" IM.illustrations_approvedBy="felipe" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.tables_requiresApprovalFor="GgImagineBatch" IM.taxonomicNames_approvedBy="felipe" IM.treatments_approvedBy="felipe" checkinTime="1720037011775" checkinUser="felipe" docAuthor="Liu, Mingjian, Fan, Yangbo, Miao, Miao, Hu, Xiaozhong, Al-Rasheid, Khaled A. S., Al-Farraj, Saleh A. & Ma, Honggang" docDate="2015" docId="03A7CC650A36FFE2FF2E81BDFCA4FE2E" docLanguage="en" docName="ActaProtozool.54.3.171-182.pdf" docOrigin="Acta Protozoologica 54 (3)" docSource="https://www.mendeley.com/catalogue/43abb6da-2026-36a8-8624-1d2b8393608c/" docStyle="DocumentStyle:64ABA4BEA387EE5C24A0DDEB32CC92E7.3:ActaProtozool.2014-.journal_article" docStyleId="64ABA4BEA387EE5C24A0DDEB32CC92E7" docStyleName="ActaProtozool.2014-.journal_article" docStyleVersion="3" docTitle="Euplotes amieti Dragesco 1970" docType="treatment" docVersion="2" lastPageNumber="178" masterDocId="FF9EB41D0A34FFE5FF84801CFF2DFFE4" masterDocTitle="Morphological and Morphogenetic Redescriptions and SSU rRNA Gene-based Phylogeny of the Poorly-known Species Euplotes amieti Dragesco, 1970 (Ciliophora, Euplotida)" masterLastPageNumber="182" masterPageNumber="171" pageNumber="173" updateTime="1720040048563" updateUser="ExternalLinkService" zenodo-license-document="CC-BY-4.0">
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<mods:title id="B45F540C5ECED94535B97207C3327B36">Morphological and Morphogenetic Redescriptions and SSU rRNA Gene-based Phylogeny of the Poorly-known Species Euplotes amieti Dragesco, 1970 (Ciliophora, Euplotida)</mods:title>
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<mods:namePart id="46B861342D7E6286D5D70F8A34E970A9">Liu, Mingjian</mods:namePart>
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<mods:namePart id="D796263800D3B0364F84D903DFC0CEB2">Fan, Yangbo</mods:namePart>
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<mods:namePart id="FA1907E6EDFB1978672D3A3B2E0A0B90">Miao, Miao</mods:namePart>
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<mods:namePart id="DE1DCC401A8461C8A4B9066516A1AA36">Hu, Xiaozhong</mods:namePart>
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<mods:namePart id="D13A5457BA267764A2B0B596E5D6354D">Al-Rasheid, Khaled A. S.</mods:namePart>
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<mods:namePart id="68C64B245D99AEB8965BE74567698746">Al-Farraj, Saleh A.</mods:namePart>
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<mods:namePart id="D4D749631267FAC590E2A9BCF176DF5E">Ma, Honggang</mods:namePart>
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<mods:title id="DC46EE6CD3B9A28FF976069BD06643A8">Acta Protozoologica</mods:title>
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<mods:date id="AD52A08A33E7B9EB06C35D1EFDB14D75">2015</mods:date>
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<mods:identifier id="E9FB7BD5641E8FD8F888C2C090C0CC80" type="DOI">10.4467/16890027AP.15.014.3211</mods:identifier>
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<mods:identifier id="05BD774BDAE8F3725F523097C8B362FA" type="ISSN">1689-0027</mods:identifier>
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<treatment id="03A7CC650A36FFE2FF2E81BDFCA4FE2E" LSID="urn:lsid:plazi:treatment:03A7CC650A36FFE2FF2E81BDFCA4FE2E" httpUri="http://treatment.plazi.org/id/03A7CC650A36FFE2FF2E81BDFCA4FE2E" lastPageId="7" lastPageNumber="178" pageId="2" pageNumber="173">
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<subSubSection id="C3142EF80A36FFE7FF2E81BDFE5DFD88" pageId="2" pageNumber="173" type="nomenclature">
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<paragraph id="8BB17D730A36FFE7FF2E81BDFFC4FE3B" blockId="2.[132,779,347,1150]" pageId="2" pageNumber="173">
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<heading id="D0F9CA1F0A36FFE7FF2E81BDFFC4FE3B" fontSize="11" level="2" pageId="2" pageNumber="173" reason="5">
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<emphasis id="B97AA1610A36FFE7FF2E81BDFFC4FE3B" bold="true" pageId="2" pageNumber="173">
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<taxonomicName id="4C0E06F00A36FFE7FF2E81BDFD12FE58" authority="Dragesco, 1970" authorityName="Dragesco" authorityYear="1970" box="[170,575,417,444]" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="2" pageNumber="173" phylum="Ciliophora" rank="species" species="amieti">
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<emphasis id="B97AA1610A36FFE7FF2E81BDFE40FE58" bold="true" box="[170,365,417,444]" italics="true" pageId="2" pageNumber="173">Euplotes amieti</emphasis>
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<bibRefCitation id="EF9F00820A36FFE7FEFF81BEFD12FE58" author="Dragesco J." box="[379,575,418,444]" pageId="2" pageNumber="173" pagination="1 - 141" refId="ref7059" refString="Dragesco J. (1970) Cilies libres du Cameroun. Ann. Fac. Sci. Jaounde (Hors serie), 1 - 141" type="book chapter" year="1970">Dragesco, 1970</bibRefCitation>
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</taxonomicName>
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(
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<figureCitation id="133561F60A36FFE7FDD081BDFD8AFE58" box="[596,679,417,444]" captionStart="Fig" captionStartId="3.[125,160,1356,1378]" captionTargetBox="[146,1439,236,1296]" captionTargetId="figure-7@3.[125,1468,223,1317]" captionTargetPageId="3" captionText="Fig. 1. Euplotes amieti Dragesco, 1970 in vivo (A), after protargol (B–D) and silver nitrate (E, F) impregnation. (A) Ventral view of a rep- resentative cell. Arrows indicate caudal cirri. (B) Different shapes of macronucleus. (C, D) Ventral (C) and dorsal (D) view, showing the infraciliature and nuclear apparatus. Arrow shows the sigmoidal adoral zone. (E, F) Silverline system on ventral (E) and dorsal side (F). Arrow shows the sigmoidal adoral zone. AZM, adoral zone of membranelles; CC, caudal cirri; CVP, contractile vacuole pore; FVC, fron- toventral cirri; MC, marginal cirri; PM, paroral membrane; TC, transverse cirri. Scale bars: 100 μm." figureDoi="http://doi.org/10.5281/zenodo.12638198" httpUri="https://zenodo.org/record/12638198/files/figure.png" pageId="2" pageNumber="173">Figs 1</figureCitation>
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and
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<figureCitation id="133561F60A36FFE7FD7681BEFC2EFE58" box="[754,771,418,444]" captionStart="Fig" captionStartId="4.[132,167,1231,1253]" captionTargetBox="[132,1470,223,1187]" captionTargetId="figure-170@4.[132,1470,223,1187]" captionTargetPageId="4" captionText="Fig. 2. Photomicrographs of Euplotes amieti Dragesco, 1970 in vivo (A–H), after protargol (I–K) and silver nitrate (L, M) impregnation. (A) Ventral view of a representative individual. Arrow points to the contractile vacuole. (B, C) Different body shapes. (D) Lateral view showing flattened body. (E) Posterior part of an individual, arrowheads show the caudal cirri and arrows indicate the left marginal cirri. (F) Numerous irregular ellipsoid to oval granules (possibly mitochondria) extremely densely packed beneath dorsal and ventral pellicle. (G) Detailed view, arrows point to the basal positions of dorsal cilia. (H) Showing the endoplasm and the typical 3-shaped macronucleus. (I) Individual with three caudal cirri (arrowheads). (J, K) Ventral (J) and dorsal (K) view of the same specimen, showing the infraciliature and nuclear apparatus. (L, M) Portion of dorsal (L) and ventral (M) silverline system. Arrow indicates the position of contractile vacuole pore. Scale bars: 100 μm." figureDoi="http://doi.org/10.5281/zenodo.12638200" httpUri="https://zenodo.org/record/12638200/files/figure.png" pageId="2" pageNumber="173">2</figureCitation>
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;
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<tableCitation id="C68C48C80A36FFE7FF0081D9FFF2FE3B" box="[132,223,453,479]" captionStart="Table 1" captionStartId="2.[132,188,1267,1288]" captionText="Table 1. Morphometric data of Euplotes amieti Dragesco, 1970." pageId="2" pageNumber="173">Table 1</tableCitation>
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)
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</emphasis>
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</heading>
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</paragraph>
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<paragraph id="8BB17D730A36FFE7FF2E81F4FE5DFD88" blockId="2.[132,779,347,1150]" pageId="2" pageNumber="173">Since this species has never been clearly diagnosed before, we present here an improved diagnosis based on the original description, the African populations and the Chinese population.</paragraph>
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</subSubSection>
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<subSubSection id="C3142EF80A36FFE7FF2E826AFE66FC2A" pageId="2" pageNumber="173" type="diagnosis">
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<paragraph id="8BB17D730A36FFE7FF2E826AFE66FC2A" blockId="2.[132,779,347,1150]" pageId="2" pageNumber="173">
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<emphasis id="B97AA1610A36FFE7FF2E826AFE83FD6B" bold="true" box="[170,430,629,656]" pageId="2" pageNumber="173">Improved diagnosis:</emphasis>
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Fresh water
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<taxonomicName id="4C0E06F00A36FFE7FDD5826AFD95FD74" authorityName="Ehrenberg" authorityYear="1830" box="[593,696,630,656]" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="2" pageNumber="173" phylum="Ciliophora" rank="genus">
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<emphasis id="B97AA1610A36FFE7FDD5826AFD95FD74" box="[593,696,630,656]" italics="true" pageId="2" pageNumber="173">Euplotes</emphasis>
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</taxonomicName>
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, about 130–240 μm × 70–160 μm
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<emphasis id="B97AA1610A36FFE7FE4E8286FD37FD57" box="[458,538,666,691]" italics="true" pageId="2" pageNumber="173">in vivo</emphasis>
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; buccal field prominent and broad, triangular in outline, about 1/2 to 2/3 of body length with about 52–70 membranelles; invari- ably nine frontoventral, five transverse and two left marginal cirri, usually two caudal cirri; 12–15 dorsal kineties with about 18–32 dikinetids in mid-dorsal row; macronucleus typically in the shape of the number three (hereafter, 3-shaped); dorsal silverline system
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<emphasis id="B97AA1610A36FFE7FD35838CFE27FC29" italics="true" pageId="2" pageNumber="173">doubleeurystomus</emphasis>
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<typeStatus id="54B5C3D10A36FFE7FE9583A8FE6AFC2A" box="[273,327,948,974]" pageId="2" pageNumber="173">type</typeStatus>
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.
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</paragraph>
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||
</subSubSection>
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||
<subSubSection id="C3142EF80A36FFE2FF2E83CAFCA4FE2E" lastPageId="7" lastPageNumber="178" pageId="2" pageNumber="173" type="description">
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||
<paragraph id="8BB17D730A36FFE7FF2E83CAFA32FC65" blockId="2.[132,779,347,1150]" lastBlockId="2.[816,1463,223,1150]" pageId="2" pageNumber="173">
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<emphasis id="B97AA1610A36FFE7FF2E83CAFD5DFC14" bold="true" box="[170,624,982,1008]" pageId="2" pageNumber="173">
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Description of
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<collectingRegion id="49CAB3910A36FFE7FEE683CAFEFAFC14" box="[354,471,982,1008]" country="China" name="Shanghai" pageId="2" pageNumber="173">Shanghai</collectingRegion>
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population:
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</emphasis>
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Cells
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<emphasis id="B97AA1610A36FFE7FD3F83CBFC26FC14" box="[699,779,983,1008]" italics="true" pageId="2" pageNumber="173">in vivo</emphasis>
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about 130–200 μm × 70–100 μm, generally asymmet- ric oval in outline; anterior end slightly rounded while posterior end often widely rounded (
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<figureCitation id="133561F60A36FFE7FDBE845CFDEFFBBE" box="[570,706,1088,1114]" captionStart="Fig" captionStartId="4.[132,167,1231,1253]" captionTargetBox="[132,1470,223,1187]" captionTargetId="figure-170@4.[132,1470,223,1187]" captionTargetPageId="4" captionText="Fig. 2. Photomicrographs of Euplotes amieti Dragesco, 1970 in vivo (A–H), after protargol (I–K) and silver nitrate (L, M) impregnation. (A) Ventral view of a representative individual. Arrow points to the contractile vacuole. (B, C) Different body shapes. (D) Lateral view showing flattened body. (E) Posterior part of an individual, arrowheads show the caudal cirri and arrows indicate the left marginal cirri. (F) Numerous irregular ellipsoid to oval granules (possibly mitochondria) extremely densely packed beneath dorsal and ventral pellicle. (G) Detailed view, arrows point to the basal positions of dorsal cilia. (H) Showing the endoplasm and the typical 3-shaped macronucleus. (I) Individual with three caudal cirri (arrowheads). (J, K) Ventral (J) and dorsal (K) view of the same specimen, showing the infraciliature and nuclear apparatus. (L, M) Portion of dorsal (L) and ventral (M) silverline system. Arrow indicates the position of contractile vacuole pore. Scale bars: 100 μm." figureDoi="http://doi.org/10.5281/zenodo.12638200" httpUri="https://zenodo.org/record/12638200/files/figure.png" pageId="2" pageNumber="173">Figs 2A, B</figureCitation>
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). Cell body dorsoventrally flattened about 2: 1 with dorsal side a little arched and ventral side concave (
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<figureCitation id="133561F60A36FFE7FACE80C3FA87FF1D" box="[1354,1450,223,249]" captionStart="Fig" captionStartId="4.[132,167,1231,1253]" captionTargetBox="[132,1470,223,1187]" captionTargetId="figure-170@4.[132,1470,223,1187]" captionTargetPageId="4" captionText="Fig. 2. Photomicrographs of Euplotes amieti Dragesco, 1970 in vivo (A–H), after protargol (I–K) and silver nitrate (L, M) impregnation. (A) Ventral view of a representative individual. Arrow points to the contractile vacuole. (B, C) Different body shapes. (D) Lateral view showing flattened body. (E) Posterior part of an individual, arrowheads show the caudal cirri and arrows indicate the left marginal cirri. (F) Numerous irregular ellipsoid to oval granules (possibly mitochondria) extremely densely packed beneath dorsal and ventral pellicle. (G) Detailed view, arrows point to the basal positions of dorsal cilia. (H) Showing the endoplasm and the typical 3-shaped macronucleus. (I) Individual with three caudal cirri (arrowheads). (J, K) Ventral (J) and dorsal (K) view of the same specimen, showing the infraciliature and nuclear apparatus. (L, M) Portion of dorsal (L) and ventral (M) silverline system. Arrow indicates the position of contractile vacuole pore. Scale bars: 100 μm." figureDoi="http://doi.org/10.5281/zenodo.12638200" httpUri="https://zenodo.org/record/12638200/files/figure.png" pageId="2" pageNumber="173">Fig. 2D</figureCitation>
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). Buccal field broad and triangular in outline, approxi- mately 1/2–2/3 of body length (
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<figureCitation id="133561F60A36FFE7FB38813BFA64FEA5" box="[1212,1353,295,321]" captionStart="Fig" captionStartId="4.[132,167,1231,1253]" captionTargetBox="[132,1470,223,1187]" captionTargetId="figure-170@4.[132,1470,223,1187]" captionTargetPageId="4" captionText="Fig. 2. Photomicrographs of Euplotes amieti Dragesco, 1970 in vivo (A–H), after protargol (I–K) and silver nitrate (L, M) impregnation. (A) Ventral view of a representative individual. Arrow points to the contractile vacuole. (B, C) Different body shapes. (D) Lateral view showing flattened body. (E) Posterior part of an individual, arrowheads show the caudal cirri and arrows indicate the left marginal cirri. (F) Numerous irregular ellipsoid to oval granules (possibly mitochondria) extremely densely packed beneath dorsal and ventral pellicle. (G) Detailed view, arrows point to the basal positions of dorsal cilia. (H) Showing the endoplasm and the typical 3-shaped macronucleus. (I) Individual with three caudal cirri (arrowheads). (J, K) Ventral (J) and dorsal (K) view of the same specimen, showing the infraciliature and nuclear apparatus. (L, M) Portion of dorsal (L) and ventral (M) silverline system. Arrow indicates the position of contractile vacuole pore. Scale bars: 100 μm." figureDoi="http://doi.org/10.5281/zenodo.12638200" httpUri="https://zenodo.org/record/12638200/files/figure.png" pageId="2" pageNumber="173">Figs 2A, B</figureCitation>
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). A conspicuous collar positioned at anterior end of dorsal side (
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<figureCitation id="133561F60A36FFE7FCF78173FCD3FE6D" box="[883,1022,367,393]" captionStart="Fig" captionStartId="4.[132,167,1231,1253]" captionTargetBox="[132,1470,223,1187]" captionTargetId="figure-170@4.[132,1470,223,1187]" captionTargetPageId="4" captionText="Fig. 2. Photomicrographs of Euplotes amieti Dragesco, 1970 in vivo (A–H), after protargol (I–K) and silver nitrate (L, M) impregnation. (A) Ventral view of a representative individual. Arrow points to the contractile vacuole. (B, C) Different body shapes. (D) Lateral view showing flattened body. (E) Posterior part of an individual, arrowheads show the caudal cirri and arrows indicate the left marginal cirri. (F) Numerous irregular ellipsoid to oval granules (possibly mitochondria) extremely densely packed beneath dorsal and ventral pellicle. (G) Detailed view, arrows point to the basal positions of dorsal cilia. (H) Showing the endoplasm and the typical 3-shaped macronucleus. (I) Individual with three caudal cirri (arrowheads). (J, K) Ventral (J) and dorsal (K) view of the same specimen, showing the infraciliature and nuclear apparatus. (L, M) Portion of dorsal (L) and ventral (M) silverline system. Arrow indicates the position of contractile vacuole pore. Scale bars: 100 μm." figureDoi="http://doi.org/10.5281/zenodo.12638200" httpUri="https://zenodo.org/record/12638200/files/figure.png" pageId="2" pageNumber="173">Figs 2A, B</figureCitation>
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). No ridges on dorsal side, but four short, inconspicuous ridges between transverse cirri on ventral side (
|
||
<figureCitation id="133561F60A36FFE7FC4F81ABFB07FE35" box="[971,1066,439,465]" captionStart="Fig" captionStartId="3.[125,160,1356,1378]" captionTargetBox="[146,1439,236,1296]" captionTargetId="figure-7@3.[125,1468,223,1317]" captionTargetPageId="3" captionText="Fig. 1. Euplotes amieti Dragesco, 1970 in vivo (A), after protargol (B–D) and silver nitrate (E, F) impregnation. (A) Ventral view of a rep- resentative cell. Arrows indicate caudal cirri. (B) Different shapes of macronucleus. (C, D) Ventral (C) and dorsal (D) view, showing the infraciliature and nuclear apparatus. Arrow shows the sigmoidal adoral zone. (E, F) Silverline system on ventral (E) and dorsal side (F). Arrow shows the sigmoidal adoral zone. AZM, adoral zone of membranelles; CC, caudal cirri; CVP, contractile vacuole pore; FVC, fron- toventral cirri; MC, marginal cirri; PM, paroral membrane; TC, transverse cirri. Scale bars: 100 μm." figureDoi="http://doi.org/10.5281/zenodo.12638198" httpUri="https://zenodo.org/record/12638198/files/figure.png" pageId="2" pageNumber="173">Fig. 1A</figureCitation>
|
||
). Numerous irregular ellipsoid to oval granules (possibly mitochondria) about 1.5–2 μm across, extremely densely packed beneath dorsal and ventral pellicle (
|
||
<figureCitation id="133561F60A36FFE7FC70823FFB7DFDD9" box="[1012,1104,547,573]" captionStart="Fig" captionStartId="4.[132,167,1231,1253]" captionTargetBox="[132,1470,223,1187]" captionTargetId="figure-170@4.[132,1470,223,1187]" captionTargetPageId="4" captionText="Fig. 2. Photomicrographs of Euplotes amieti Dragesco, 1970 in vivo (A–H), after protargol (I–K) and silver nitrate (L, M) impregnation. (A) Ventral view of a representative individual. Arrow points to the contractile vacuole. (B, C) Different body shapes. (D) Lateral view showing flattened body. (E) Posterior part of an individual, arrowheads show the caudal cirri and arrows indicate the left marginal cirri. (F) Numerous irregular ellipsoid to oval granules (possibly mitochondria) extremely densely packed beneath dorsal and ventral pellicle. (G) Detailed view, arrows point to the basal positions of dorsal cilia. (H) Showing the endoplasm and the typical 3-shaped macronucleus. (I) Individual with three caudal cirri (arrowheads). (J, K) Ventral (J) and dorsal (K) view of the same specimen, showing the infraciliature and nuclear apparatus. (L, M) Portion of dorsal (L) and ventral (M) silverline system. Arrow indicates the position of contractile vacuole pore. Scale bars: 100 μm." figureDoi="http://doi.org/10.5281/zenodo.12638200" httpUri="https://zenodo.org/record/12638200/files/figure.png" pageId="2" pageNumber="173">Fig. 2F</figureCitation>
|
||
); about nine bar-shaped granules, ca. 1 μm long, densely packed around dorsal cilia beneath pellicle. Cytoplasm colorless, highly transpar- ent at marginal area, and containing several to many different-sized lipid droplets and several food vacuoles (1.5–2 μm in diameter) in central part (
|
||
<figureCitation id="133561F60A36FFE7FA8B82CBFA59FD15" box="[1295,1396,727,753]" captionStart="Fig" captionStartId="3.[125,160,1356,1378]" captionTargetBox="[146,1439,236,1296]" captionTargetId="figure-7@3.[125,1468,223,1317]" captionTargetPageId="3" captionText="Fig. 1. Euplotes amieti Dragesco, 1970 in vivo (A), after protargol (B–D) and silver nitrate (E, F) impregnation. (A) Ventral view of a rep- resentative cell. Arrows indicate caudal cirri. (B) Different shapes of macronucleus. (C, D) Ventral (C) and dorsal (D) view, showing the infraciliature and nuclear apparatus. Arrow shows the sigmoidal adoral zone. (E, F) Silverline system on ventral (E) and dorsal side (F). Arrow shows the sigmoidal adoral zone. AZM, adoral zone of membranelles; CC, caudal cirri; CVP, contractile vacuole pore; FVC, fron- toventral cirri; MC, marginal cirri; PM, paroral membrane; TC, transverse cirri. Scale bars: 100 μm." figureDoi="http://doi.org/10.5281/zenodo.12638198" httpUri="https://zenodo.org/record/12638198/files/figure.png" pageId="2" pageNumber="173">Figs 1A</figureCitation>
|
||
,
|
||
<figureCitation id="133561F60A36FFE7FA0782CBFA84FD15" box="[1411,1449,727,753]" captionStart="Fig" captionStartId="4.[132,167,1231,1253]" captionTargetBox="[132,1470,223,1187]" captionTargetId="figure-170@4.[132,1470,223,1187]" captionTargetPageId="4" captionText="Fig. 2. Photomicrographs of Euplotes amieti Dragesco, 1970 in vivo (A–H), after protargol (I–K) and silver nitrate (L, M) impregnation. (A) Ventral view of a representative individual. Arrow points to the contractile vacuole. (B, C) Different body shapes. (D) Lateral view showing flattened body. (E) Posterior part of an individual, arrowheads show the caudal cirri and arrows indicate the left marginal cirri. (F) Numerous irregular ellipsoid to oval granules (possibly mitochondria) extremely densely packed beneath dorsal and ventral pellicle. (G) Detailed view, arrows point to the basal positions of dorsal cilia. (H) Showing the endoplasm and the typical 3-shaped macronucleus. (I) Individual with three caudal cirri (arrowheads). (J, K) Ventral (J) and dorsal (K) view of the same specimen, showing the infraciliature and nuclear apparatus. (L, M) Portion of dorsal (L) and ventral (M) silverline system. Arrow indicates the position of contractile vacuole pore. Scale bars: 100 μm." figureDoi="http://doi.org/10.5281/zenodo.12638200" httpUri="https://zenodo.org/record/12638200/files/figure.png" pageId="2" pageNumber="173">2B</figureCitation>
|
||
). One contractile vacuole 25–30 μm in diameter, adjacent to the rightmost transverse cirrus (
|
||
<figureCitation id="133561F60A36FFE7FB3A8303FA32FCDE" box="[1214,1311,799,826]" captionStart="Fig" captionStartId="3.[125,160,1356,1378]" captionTargetBox="[146,1439,236,1296]" captionTargetId="figure-7@3.[125,1468,223,1317]" captionTargetPageId="3" captionText="Fig. 1. Euplotes amieti Dragesco, 1970 in vivo (A), after protargol (B–D) and silver nitrate (E, F) impregnation. (A) Ventral view of a rep- resentative cell. Arrows indicate caudal cirri. (B) Different shapes of macronucleus. (C, D) Ventral (C) and dorsal (D) view, showing the infraciliature and nuclear apparatus. Arrow shows the sigmoidal adoral zone. (E, F) Silverline system on ventral (E) and dorsal side (F). Arrow shows the sigmoidal adoral zone. AZM, adoral zone of membranelles; CC, caudal cirri; CVP, contractile vacuole pore; FVC, fron- toventral cirri; MC, marginal cirri; PM, paroral membrane; TC, transverse cirri. Scale bars: 100 μm." figureDoi="http://doi.org/10.5281/zenodo.12638198" httpUri="https://zenodo.org/record/12638198/files/figure.png" pageId="2" pageNumber="173">Figs 1A</figureCitation>
|
||
,
|
||
<figureCitation id="133561F60A36FFE7FAAE833CFA7FFCDE" box="[1322,1362,800,826]" captionStart="Fig" captionStartId="4.[132,167,1231,1253]" captionTargetBox="[132,1470,223,1187]" captionTargetId="figure-170@4.[132,1470,223,1187]" captionTargetPageId="4" captionText="Fig. 2. Photomicrographs of Euplotes amieti Dragesco, 1970 in vivo (A–H), after protargol (I–K) and silver nitrate (L, M) impregnation. (A) Ventral view of a representative individual. Arrow points to the contractile vacuole. (B, C) Different body shapes. (D) Lateral view showing flattened body. (E) Posterior part of an individual, arrowheads show the caudal cirri and arrows indicate the left marginal cirri. (F) Numerous irregular ellipsoid to oval granules (possibly mitochondria) extremely densely packed beneath dorsal and ventral pellicle. (G) Detailed view, arrows point to the basal positions of dorsal cilia. (H) Showing the endoplasm and the typical 3-shaped macronucleus. (I) Individual with three caudal cirri (arrowheads). (J, K) Ventral (J) and dorsal (K) view of the same specimen, showing the infraciliature and nuclear apparatus. (L, M) Portion of dorsal (L) and ventral (M) silverline system. Arrow indicates the position of contractile vacuole pore. Scale bars: 100 μm." figureDoi="http://doi.org/10.5281/zenodo.12638200" httpUri="https://zenodo.org/record/12638200/files/figure.png" pageId="2" pageNumber="173">2A</figureCitation>
|
||
); pulsat- ing at intervals of 1–2 min. Locomotion typically by moderately fast crawling or slight jerking.
|
||
</paragraph>
|
||
<paragraph id="8BB17D730A36FFE7FCD28397FA9AFB99" blockId="2.[816,1463,223,1150]" pageId="2" pageNumber="173">Adoral zone prominent, composed of 60–65 membranelles, commencing at anterior part of frontoventral cirri on ventral side with ca. five membranelles, extending to collar zone on dorsal side with ca. 16 membranelles. Other membranelles extending from collar zone to ventral side with about a 90-degree curve and occupying 1/2–2/3 body length in a sigmoidal shape</paragraph>
|
||
<caption id="DF712DFB0A36FFE7FF0084EFFDC0FAEC" box="[132,749,1266,1288]" pageId="2" pageNumber="173" startId="2.[132,188,1267,1288]" targetType="table">
|
||
<paragraph id="8BB17D730A36FFE7FF0084EFFDC0FAEC" blockId="2.[132,749,1266,1288]" box="[132,749,1266,1288]" pageId="2" pageNumber="173">
|
||
<emphasis id="B97AA1610A36FFE7FF0084EFFFF8FAE3" bold="true" box="[132,213,1266,1288]" pageId="2" pageNumber="173">Table 1.</emphasis>
|
||
Morphometric data of
|
||
<taxonomicName id="4C0E06F00A36FFE7FE3784EEFDC4FAEC" authority="Dragesco, 1970" authorityName="Dragesco" authorityYear="1970" box="[435,745,1266,1288]" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="2" pageNumber="173" phylum="Ciliophora" rank="species" species="amieti">
|
||
<emphasis id="B97AA1610A36FFE7FE3784EEFD64FAEC" box="[435,585,1266,1288]" italics="true" pageId="2" pageNumber="173">Euplotes amieti</emphasis>
|
||
<bibRefCitation id="EF9F00820A36FFE7FDCB84EFFDC4FAEC" author="Dragesco J." box="[591,745,1267,1288]" pageId="2" pageNumber="173" pagination="1 - 141" refId="ref7059" refString="Dragesco J. (1970) Cilies libres du Cameroun. Ann. Fac. Sci. Jaounde (Hors serie), 1 - 141" type="book chapter" year="1970">Dragesco, 1970</bibRefCitation>
|
||
</taxonomicName>
|
||
.
|
||
</paragraph>
|
||
</caption>
|
||
<paragraph id="8BB17D730A36FFE7FF008535FAADF8AC" pageId="2" pageNumber="173">
|
||
<table id="F90E8FD30A36001AFF008535FAADF8AC" box="[132,1408,1321,1864]" gridcols="7" gridrows="15" pageId="2" pageNumber="173">
|
||
<tr id="353E7F310A36001AFF008535FAADFAD8" box="[132,1408,1321,1340]" gridrow="0" pageId="2" pageNumber="173">
|
||
<th id="76EF164D0A36001AFF008535FD3DFAD8" box="[132,528,1321,1340]" gridcol="0" gridrow="0" pageId="2" pageNumber="173">Characteristics</th>
|
||
<th id="76EF164D0A36001AFD538535FC2CFAD8" box="[727,769,1321,1340]" gridcol="1" gridrow="0" pageId="2" pageNumber="173">Max</th>
|
||
<th id="76EF164D0A36001AFCDD8535FCACFAD8" box="[857,897,1321,1340]" gridcol="2" gridrow="0" pageId="2" pageNumber="173">Min</th>
|
||
<th id="76EF164D0A36001AFC578535FB23FAD8" box="[979,1038,1321,1340]" gridcol="3" gridrow="0" pageId="2" pageNumber="173">Mean</th>
|
||
<th id="76EF164D0A36001AFBD88535FBA0FAD8" box="[1116,1165,1321,1340]" gridcol="4" gridrow="0" pageId="2" pageNumber="173">SD</th>
|
||
<th id="76EF164D0A36001AFB5A8535FA29FAD8" box="[1246,1284,1321,1340]" gridcol="5" gridrow="0" pageId="2" pageNumber="173">CV</th>
|
||
<th id="76EF164D0A36001AFAEF8535FAADFAD8" box="[1387,1408,1321,1340]" gridcol="6" gridrow="0" pageId="2" pageNumber="173">n</th>
|
||
</tr>
|
||
<tr id="353E7F310A36001AFF008552FAADFA85" box="[132,1408,1358,1377]" gridrow="1" pageId="2" pageNumber="173">
|
||
<th id="76EF164D0A36001AFF008552FD3DFA85" box="[132,528,1358,1377]" gridcol="0" gridrow="1" pageId="2" pageNumber="173">Body length</th>
|
||
<td id="76EF164D0A36001AFD538552FC2CFA85" box="[727,769,1358,1377]" gridcol="1" gridrow="1" pageId="2" pageNumber="173">165</td>
|
||
<td id="76EF164D0A36001AFCDD8552FCACFA85" box="[857,897,1358,1377]" gridcol="2" gridrow="1" pageId="2" pageNumber="173">122</td>
|
||
<td id="76EF164D0A36001AFC578552FB23FA85" box="[979,1038,1358,1377]" gridcol="3" gridrow="1" pageId="2" pageNumber="173">142.50</td>
|
||
<td id="76EF164D0A36001AFBD88552FBA0FA85" box="[1116,1165,1358,1377]" gridcol="4" gridrow="1" pageId="2" pageNumber="173">10.27</td>
|
||
<td id="76EF164D0A36001AFB5A8552FA29FA85" box="[1246,1284,1358,1377]" gridcol="5" gridrow="1" pageId="2" pageNumber="173">7.2</td>
|
||
<td id="76EF164D0A36001AFAEF8552FAADFA85" box="[1387,1408,1358,1377]" gridcol="6" gridrow="1" pageId="2" pageNumber="173">25</td>
|
||
</tr>
|
||
<tr id="353E7F310A36001AFF008568FAADFA63" box="[132,1408,1396,1415]" gridrow="2" pageId="2" pageNumber="173">
|
||
<th id="76EF164D0A36001AFF008568FD3DFA63" box="[132,528,1396,1415]" gridcol="0" gridrow="2" pageId="2" pageNumber="173">Body width</th>
|
||
<td id="76EF164D0A36001AFD538568FC2CFA63" box="[727,769,1396,1415]" gridcol="1" gridrow="2" pageId="2" pageNumber="173">114</td>
|
||
<td id="76EF164D0A36001AFCDD8568FCACFA63" box="[857,897,1396,1415]" gridcol="2" gridrow="2" pageId="2" pageNumber="173">78</td>
|
||
<td id="76EF164D0A36001AFC578568FB23FA63" box="[979,1038,1396,1415]" gridcol="3" gridrow="2" pageId="2" pageNumber="173">97.20</td>
|
||
<td id="76EF164D0A36001AFBD88568FBA0FA63" box="[1116,1165,1396,1415]" gridcol="4" gridrow="2" pageId="2" pageNumber="173">8.89</td>
|
||
<td id="76EF164D0A36001AFB5A8568FA29FA63" box="[1246,1284,1396,1415]" gridcol="5" gridrow="2" pageId="2" pageNumber="173">9.1</td>
|
||
<td id="76EF164D0A36001AFAEF8568FAADFA63" box="[1387,1408,1396,1415]" gridcol="6" gridrow="2" pageId="2" pageNumber="173">25</td>
|
||
</tr>
|
||
<tr id="353E7F310A36001AFF008585FAADFA48" box="[132,1408,1433,1452]" gridrow="3" pageId="2" pageNumber="173">
|
||
<th id="76EF164D0A36001AFF008585FD3DFA48" box="[132,528,1433,1452]" gridcol="0" gridrow="3" pageId="2" pageNumber="173">Length of buccal field</th>
|
||
<td id="76EF164D0A36001AFD538585FC2CFA48" box="[727,769,1433,1452]" gridcol="1" gridrow="3" pageId="2" pageNumber="173">100</td>
|
||
<td id="76EF164D0A36001AFCDD8585FCACFA48" box="[857,897,1433,1452]" gridcol="2" gridrow="3" pageId="2" pageNumber="173">87</td>
|
||
<td id="76EF164D0A36001AFC578585FB23FA48" box="[979,1038,1433,1452]" gridcol="3" gridrow="3" pageId="2" pageNumber="173">94.64</td>
|
||
<td id="76EF164D0A36001AFBD88585FBA0FA48" box="[1116,1165,1433,1452]" gridcol="4" gridrow="3" pageId="2" pageNumber="173">4.21</td>
|
||
<td id="76EF164D0A36001AFB5A8585FA29FA48" box="[1246,1284,1433,1452]" gridcol="5" gridrow="3" pageId="2" pageNumber="173">4.5</td>
|
||
<td id="76EF164D0A36001AFAEF8585FAADFA48" box="[1387,1408,1433,1452]" gridcol="6" gridrow="3" pageId="2" pageNumber="173">25</td>
|
||
</tr>
|
||
<tr id="353E7F310A36001AFF0085A3FAADFA36" box="[132,1408,1471,1490]" gridrow="4" pageId="2" pageNumber="173">
|
||
<th id="76EF164D0A36001AFF0085A3FD3DFA36" box="[132,528,1471,1490]" gridcol="0" gridrow="4" pageId="2" pageNumber="173">Length of buccal field: body length</th>
|
||
<td id="76EF164D0A36001AFD5385A3FC2CFA36" box="[727,769,1471,1490]" gridcol="1" gridrow="4" pageId="2" pageNumber="173">0.74</td>
|
||
<td id="76EF164D0A36001AFCDD85A3FCACFA36" box="[857,897,1471,1490]" gridcol="2" gridrow="4" pageId="2" pageNumber="173">0.59</td>
|
||
<td id="76EF164D0A36001AFC5785A3FB23FA36" box="[979,1038,1471,1490]" gridcol="3" gridrow="4" pageId="2" pageNumber="173">0.67</td>
|
||
<td id="76EF164D0A36001AFBD885A3FBA0FA36" box="[1116,1165,1471,1490]" gridcol="4" gridrow="4" pageId="2" pageNumber="173">0.05</td>
|
||
<td id="76EF164D0A36001AFB5A85A3FA29FA36" box="[1246,1284,1471,1490]" gridcol="5" gridrow="4" pageId="2" pageNumber="173">7.7</td>
|
||
<td id="76EF164D0A36001AFAEF85A3FAADFA36" box="[1387,1408,1471,1490]" gridcol="6" gridrow="4" pageId="2" pageNumber="173">25</td>
|
||
</tr>
|
||
<tr id="353E7F310A36001AFF0085F8FAADFA13" box="[132,1408,1508,1527]" gridrow="5" pageId="2" pageNumber="173">
|
||
<th id="76EF164D0A36001AFF0085F8FD3DFA13" box="[132,528,1508,1527]" gridcol="0" gridrow="5" pageId="2" pageNumber="173">Length of paroral membrane</th>
|
||
<td id="76EF164D0A36001AFD5385F8FC2CFA13" box="[727,769,1508,1527]" gridcol="1" gridrow="5" pageId="2" pageNumber="173">24</td>
|
||
<td id="76EF164D0A36001AFCDD85F8FCACFA13" box="[857,897,1508,1527]" gridcol="2" gridrow="5" pageId="2" pageNumber="173">17</td>
|
||
<td id="76EF164D0A36001AFC5785F8FB23FA13" box="[979,1038,1508,1527]" gridcol="3" gridrow="5" pageId="2" pageNumber="173">20.44</td>
|
||
<td id="76EF164D0A36001AFBD885F8FBA0FA13" box="[1116,1165,1508,1527]" gridcol="4" gridrow="5" pageId="2" pageNumber="173">1.53</td>
|
||
<td id="76EF164D0A36001AFB5A85F8FA29FA13" box="[1246,1284,1508,1527]" gridcol="5" gridrow="5" pageId="2" pageNumber="173">7.5</td>
|
||
<td id="76EF164D0A36001AFAEF85F8FAADFA13" box="[1387,1408,1508,1527]" gridcol="6" gridrow="5" pageId="2" pageNumber="173">25</td>
|
||
</tr>
|
||
<tr id="353E7F310A36001AFF008615FAADF9F9" box="[132,1408,1545,1565]" gridrow="6" pageId="2" pageNumber="173">
|
||
<th id="76EF164D0A36001AFF008615FD3DF9F9" box="[132,528,1545,1565]" gridcol="0" gridrow="6" pageId="2" pageNumber="173">Width of paroral membrane</th>
|
||
<td id="76EF164D0A36001AFD538615FC2CF9F9" box="[727,769,1545,1565]" gridcol="1" gridrow="6" pageId="2" pageNumber="173">4</td>
|
||
<td id="76EF164D0A36001AFCDD8615FCACF9F9" box="[857,897,1545,1565]" gridcol="2" gridrow="6" pageId="2" pageNumber="173">3</td>
|
||
<td id="76EF164D0A36001AFC578615FB23F9F9" box="[979,1038,1545,1565]" gridcol="3" gridrow="6" pageId="2" pageNumber="173">3.60</td>
|
||
<td id="76EF164D0A36001AFBD88615FBA0F9F9" box="[1116,1165,1545,1565]" gridcol="4" gridrow="6" pageId="2" pageNumber="173">0.50</td>
|
||
<td id="76EF164D0A36001AFB5A8615FA29F9F9" box="[1246,1284,1545,1565]" gridcol="5" gridrow="6" pageId="2" pageNumber="173">13.9</td>
|
||
<td id="76EF164D0A36001AFAEF8615FAADF9F9" box="[1387,1408,1545,1565]" gridcol="6" gridrow="6" pageId="2" pageNumber="173">25</td>
|
||
</tr>
|
||
<tr id="353E7F310A36001AFF008633FAADF9A6" box="[132,1408,1583,1602]" gridrow="7" pageId="2" pageNumber="173">
|
||
<th id="76EF164D0A36001AFF008633FD3DF9A6" box="[132,528,1583,1602]" gridcol="0" gridrow="7" pageId="2" pageNumber="173">Number of adoral membranelles</th>
|
||
<td id="76EF164D0A36001AFD538633FC2CF9A6" box="[727,769,1583,1602]" gridcol="1" gridrow="7" pageId="2" pageNumber="173">65</td>
|
||
<td id="76EF164D0A36001AFCDD8633FCACF9A6" box="[857,897,1583,1602]" gridcol="2" gridrow="7" pageId="2" pageNumber="173">60</td>
|
||
<td id="76EF164D0A36001AFC578633FB23F9A6" box="[979,1038,1583,1602]" gridcol="3" gridrow="7" pageId="2" pageNumber="173">61.80</td>
|
||
<td id="76EF164D0A36001AFBD88633FBA0F9A6" box="[1116,1165,1583,1602]" gridcol="4" gridrow="7" pageId="2" pageNumber="173">2.25</td>
|
||
<td id="76EF164D0A36001AFB5A8633FA29F9A6" box="[1246,1284,1583,1602]" gridcol="5" gridrow="7" pageId="2" pageNumber="173">3.6</td>
|
||
<td id="76EF164D0A36001AFAEF8633FAADF9A6" box="[1387,1408,1583,1602]" gridcol="6" gridrow="7" pageId="2" pageNumber="173">25</td>
|
||
</tr>
|
||
<tr id="353E7F310A36001AFF008648FAADF98C" box="[132,1408,1620,1640]" gridrow="8" pageId="2" pageNumber="173">
|
||
<th id="76EF164D0A36001AFF008648FD3DF98C" box="[132,528,1620,1640]" gridcol="0" gridrow="8" pageId="2" pageNumber="173">Number of dorsal kineties</th>
|
||
<td id="76EF164D0A36001AFD538648FC2CF98C" box="[727,769,1620,1640]" gridcol="1" gridrow="8" pageId="2" pageNumber="173">14</td>
|
||
<td id="76EF164D0A36001AFCDD8648FCACF98C" box="[857,897,1620,1640]" gridcol="2" gridrow="8" pageId="2" pageNumber="173">12</td>
|
||
<td id="76EF164D0A36001AFC578648FB23F98C" box="[979,1038,1620,1640]" gridcol="3" gridrow="8" pageId="2" pageNumber="173">13.10</td>
|
||
<td id="76EF164D0A36001AFBD88648FBA0F98C" box="[1116,1165,1620,1640]" gridcol="4" gridrow="8" pageId="2" pageNumber="173">0.46</td>
|
||
<td id="76EF164D0A36001AFB5A8648FA29F98C" box="[1246,1284,1620,1640]" gridcol="5" gridrow="8" pageId="2" pageNumber="173">3.5</td>
|
||
<td id="76EF164D0A36001AFAEF8648FAADF98C" box="[1387,1408,1620,1640]" gridcol="6" gridrow="8" pageId="2" pageNumber="173">39</td>
|
||
</tr>
|
||
<tr id="353E7F310A36001AFF008666FAADF969" box="[132,1408,1658,1677]" gridrow="9" pageId="2" pageNumber="173">
|
||
<th id="76EF164D0A36001AFF008666FD3DF969" box="[132,528,1658,1677]" gridcol="0" gridrow="9" pageId="2" pageNumber="173">Number of frontoventral cirri</th>
|
||
<td id="76EF164D0A36001AFD538666FC2CF969" box="[727,769,1658,1677]" gridcol="1" gridrow="9" pageId="2" pageNumber="173">9</td>
|
||
<td id="76EF164D0A36001AFCDD8666FCACF969" box="[857,897,1658,1677]" gridcol="2" gridrow="9" pageId="2" pageNumber="173">9</td>
|
||
<td id="76EF164D0A36001AFC578666FB23F969" box="[979,1038,1658,1677]" gridcol="3" gridrow="9" pageId="2" pageNumber="173">9.00</td>
|
||
<td id="76EF164D0A36001AFBD88666FBA0F969" box="[1116,1165,1658,1677]" gridcol="4" gridrow="9" pageId="2" pageNumber="173">0</td>
|
||
<td id="76EF164D0A36001AFB5A8666FA29F969" box="[1246,1284,1658,1677]" gridcol="5" gridrow="9" pageId="2" pageNumber="173">0</td>
|
||
<td id="76EF164D0A36001AFAEF8666FAADF969" box="[1387,1408,1658,1677]" gridcol="6" gridrow="9" pageId="2" pageNumber="173">25</td>
|
||
</tr>
|
||
<tr id="353E7F310A36001AFF008683FAADF957" box="[132,1408,1695,1715]" gridrow="10" pageId="2" pageNumber="173">
|
||
<th id="76EF164D0A36001AFF008683FD3DF957" box="[132,528,1695,1715]" gridcol="0" gridrow="10" pageId="2" pageNumber="173">Number of transverse cirri</th>
|
||
<td id="76EF164D0A36001AFD538683FC2CF957" box="[727,769,1695,1715]" gridcol="1" gridrow="10" pageId="2" pageNumber="173">5</td>
|
||
<td id="76EF164D0A36001AFCDD8683FCACF957" box="[857,897,1695,1715]" gridcol="2" gridrow="10" pageId="2" pageNumber="173">5</td>
|
||
<td id="76EF164D0A36001AFC578683FB23F957" box="[979,1038,1695,1715]" gridcol="3" gridrow="10" pageId="2" pageNumber="173">5.00</td>
|
||
<td id="76EF164D0A36001AFBD88683FBA0F957" box="[1116,1165,1695,1715]" gridcol="4" gridrow="10" pageId="2" pageNumber="173">0</td>
|
||
<td id="76EF164D0A36001AFB5A8683FA29F957" box="[1246,1284,1695,1715]" gridcol="5" gridrow="10" pageId="2" pageNumber="173">0</td>
|
||
<td id="76EF164D0A36001AFAEF8683FAADF957" box="[1387,1408,1695,1715]" gridcol="6" gridrow="10" pageId="2" pageNumber="173">25</td>
|
||
</tr>
|
||
<tr id="353E7F310A36001AFF0086D9FAADF93C" box="[132,1408,1733,1752]" gridrow="11" pageId="2" pageNumber="173">
|
||
<th id="76EF164D0A36001AFF0086D9FD3DF93C" box="[132,528,1733,1752]" gridcol="0" gridrow="11" pageId="2" pageNumber="173">Number of marginal cirri</th>
|
||
<td id="76EF164D0A36001AFD5386D9FC2CF93C" box="[727,769,1733,1752]" gridcol="1" gridrow="11" pageId="2" pageNumber="173">2</td>
|
||
<td id="76EF164D0A36001AFCDD86D9FCACF93C" box="[857,897,1733,1752]" gridcol="2" gridrow="11" pageId="2" pageNumber="173">2</td>
|
||
<td id="76EF164D0A36001AFC5786D9FB23F93C" box="[979,1038,1733,1752]" gridcol="3" gridrow="11" pageId="2" pageNumber="173">2.00</td>
|
||
<td id="76EF164D0A36001AFBD886D9FBA0F93C" box="[1116,1165,1733,1752]" gridcol="4" gridrow="11" pageId="2" pageNumber="173">0</td>
|
||
<td id="76EF164D0A36001AFB5A86D9FA29F93C" box="[1246,1284,1733,1752]" gridcol="5" gridrow="11" pageId="2" pageNumber="173">0</td>
|
||
<td id="76EF164D0A36001AFAEF86D9FAADF93C" box="[1387,1408,1733,1752]" gridcol="6" gridrow="11" pageId="2" pageNumber="173">25</td>
|
||
</tr>
|
||
<tr id="353E7F310A36001AFF0086F6FAADF91A" box="[132,1408,1770,1790]" gridrow="12" pageId="2" pageNumber="173">
|
||
<th id="76EF164D0A36001AFF0086F6FD3DF91A" box="[132,528,1770,1790]" gridcol="0" gridrow="12" pageId="2" pageNumber="173">Number of caudal cirri</th>
|
||
<td id="76EF164D0A36001AFD5386F6FC2CF91A" box="[727,769,1770,1790]" gridcol="1" gridrow="12" pageId="2" pageNumber="173">3</td>
|
||
<td id="76EF164D0A36001AFCDD86F6FCACF91A" box="[857,897,1770,1790]" gridcol="2" gridrow="12" pageId="2" pageNumber="173">2</td>
|
||
<td id="76EF164D0A36001AFC5786F6FB23F91A" box="[979,1038,1770,1790]" gridcol="3" gridrow="12" pageId="2" pageNumber="173">2.10</td>
|
||
<td id="76EF164D0A36001AFBD886F6FBA0F91A" box="[1116,1165,1770,1790]" gridcol="4" gridrow="12" pageId="2" pageNumber="173">0.27</td>
|
||
<td id="76EF164D0A36001AFB5A86F6FA29F91A" box="[1246,1284,1770,1790]" gridcol="5" gridrow="12" pageId="2" pageNumber="173">13.0</td>
|
||
<td id="76EF164D0A36001AFAEF86F6FAADF91A" box="[1387,1408,1770,1790]" gridcol="6" gridrow="12" pageId="2" pageNumber="173">39</td>
|
||
</tr>
|
||
<tr id="353E7F310A36001AFF00870CFAADF8C7" box="[132,1408,1808,1827]" gridrow="13" pageId="2" pageNumber="173">
|
||
<th id="76EF164D0A36001AFF00870CFD3DF8C7" box="[132,528,1808,1827]" gridcol="0" gridrow="13" pageId="2" pageNumber="173">Number of dikinetids in mid-dorsal kinety</th>
|
||
<td id="76EF164D0A36001AFD53870CFC2CF8C7" box="[727,769,1808,1827]" gridcol="1" gridrow="13" pageId="2" pageNumber="173">26</td>
|
||
<td id="76EF164D0A36001AFCDD870CFCACF8C7" box="[857,897,1808,1827]" gridcol="2" gridrow="13" pageId="2" pageNumber="173">18</td>
|
||
<td id="76EF164D0A36001AFC57870CFB23F8C7" box="[979,1038,1808,1827]" gridcol="3" gridrow="13" pageId="2" pageNumber="173">21.80</td>
|
||
<td id="76EF164D0A36001AFBD8870CFBA0F8C7" box="[1116,1165,1808,1827]" gridcol="4" gridrow="13" pageId="2" pageNumber="173">1.78</td>
|
||
<td id="76EF164D0A36001AFB5A870CFA29F8C7" box="[1246,1284,1808,1827]" gridcol="5" gridrow="13" pageId="2" pageNumber="173">8.2</td>
|
||
<td id="76EF164D0A36001AFAEF870CFAADF8C7" box="[1387,1408,1808,1827]" gridcol="6" gridrow="13" pageId="2" pageNumber="173">25</td>
|
||
</tr>
|
||
<tr id="353E7F310A36001AFF008729FAADF8AC" box="[132,1408,1845,1864]" gridrow="14" pageId="2" pageNumber="173">
|
||
<th id="76EF164D0A36001AFF008729FD3DF8AC" box="[132,528,1845,1864]" gridcol="0" gridrow="14" pageId="2" pageNumber="173">Number of dikinetids in leftmost dorsal kinety</th>
|
||
<td id="76EF164D0A36001AFD538729FC2CF8AC" box="[727,769,1845,1864]" gridcol="1" gridrow="14" pageId="2" pageNumber="173">20</td>
|
||
<td id="76EF164D0A36001AFCDD8729FCACF8AC" box="[857,897,1845,1864]" gridcol="2" gridrow="14" pageId="2" pageNumber="173">11</td>
|
||
<td id="76EF164D0A36001AFC578729FB23F8AC" box="[979,1038,1845,1864]" gridcol="3" gridrow="14" pageId="2" pageNumber="173">17.00</td>
|
||
<td id="76EF164D0A36001AFBD88729FBA0F8AC" box="[1116,1165,1845,1864]" gridcol="4" gridrow="14" pageId="2" pageNumber="173">2.14</td>
|
||
<td id="76EF164D0A36001AFB5A8729FA29F8AC" box="[1246,1284,1845,1864]" gridcol="5" gridrow="14" pageId="2" pageNumber="173">12.6</td>
|
||
<td id="76EF164D0A36001AFAEF8729FAADF8AC" box="[1387,1408,1845,1864]" gridcol="6" gridrow="14" pageId="2" pageNumber="173">25</td>
|
||
</tr>
|
||
</table>
|
||
</paragraph>
|
||
<paragraph id="8BB17D730A36FFE7FF008774FD84F872" blockId="2.[132,1463,1896,1942]" pageId="2" pageNumber="173">Data are based on protargol-impregnated specimens. Measurements in μm. CV, coefficient of variation in %; Max, maximum; Mean, arithmetic mean; Min, minimum; n, number of cells measured; SD, standard deviation.</paragraph>
|
||
<caption id="DF712DFB0A37FFE6FFF98550FB10FA36" ID-DOI="http://doi.org/10.5281/zenodo.12638198" ID-Zenodo-Dep="12638198" httpUri="https://zenodo.org/record/12638198/files/figure.png" pageId="3" pageNumber="174" startId="3.[125,160,1356,1378]" targetBox="[146,1439,236,1296]" targetPageId="3" targetType="figure">
|
||
<paragraph id="8BB17D730A37FFE6FFF98550FB10FA36" blockId="3.[125,1456,1356,1491]" pageId="3" pageNumber="174">
|
||
<emphasis id="B97AA1610A37FFE6FFF98550FF90FA85" bold="true" box="[125,189,1356,1378]" pageId="3" pageNumber="174">Fig. 1.</emphasis>
|
||
<taxonomicName id="4C0E06F00A37FFE6FF468551FEDBFA86" authority="Dragesco, 1970" authorityName="Dragesco" authorityYear="1970" box="[194,502,1357,1378]" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="3" pageNumber="174" phylum="Ciliophora" rank="species" species="amieti">
|
||
<emphasis id="B97AA1610A37FFE6FF468551FE74FA86" box="[194,345,1357,1378]" italics="true" pageId="3" pageNumber="174">Euplotes amieti</emphasis>
|
||
<bibRefCitation id="EF9F00820A37FFE6FEDB8551FEDBFA86" author="Dragesco J." box="[351,502,1357,1378]" pageId="3" pageNumber="174" pagination="1 - 141" refId="ref7059" refString="Dragesco J. (1970) Cilies libres du Cameroun. Ann. Fac. Sci. Jaounde (Hors serie), 1 - 141" type="book chapter" year="1970">Dragesco, 1970</bibRefCitation>
|
||
</taxonomicName>
|
||
<emphasis id="B97AA1610A37FFE6FE788551FD10FA86" box="[508,573,1357,1378]" italics="true" pageId="3" pageNumber="174">in vivo</emphasis>
|
||
(A), after protargol (B–D) and silver nitrate (E, F) impregnation. (
|
||
<emphasis id="B97AA1610A37FFE6FB3A8550FBE2FA85" bold="true" box="[1214,1231,1356,1377]" pageId="3" pageNumber="174">A</emphasis>
|
||
) Ventral view of a representative cell. Arrows indicate caudal cirri. (
|
||
<emphasis id="B97AA1610A37FFE6FDBB8575FD62FA9A" bold="true" box="[575,591,1385,1406]" pageId="3" pageNumber="174">B</emphasis>
|
||
) Different shapes of macronucleus. (
|
||
<emphasis id="B97AA1610A37FFE6FC3D8574FCC4FA9A" bold="true" box="[953,1001,1384,1406]" pageId="3" pageNumber="174">C, D</emphasis>
|
||
) Ventral (C) and dorsal (D) view, showing the infraciliature and nuclear apparatus. Arrow shows the sigmoidal adoral zone. (
|
||
<emphasis id="B97AA1610A37FFE6FCFD8599FC88FA7E" bold="true" box="[889,933,1413,1434]" pageId="3" pageNumber="174">E, F</emphasis>
|
||
) Silverline system on ventral (E) and dorsal side (F). Arrow shows the sigmoidal adoral zone. AZM, adoral zone of membranelles; CC, caudal cirri; CVP, contractile vacuole pore; FVC, frontoventral cirri; MC, marginal cirri; PM, paroral membrane; TC, transverse cirri. Scale bars: 100 μm.
|
||
</paragraph>
|
||
</caption>
|
||
<paragraph id="8BB17D730A37FFE1FFF98643FDA6F927" blockId="3.[125,771,1631,1935]" lastBlockId="4.[132,780,1601,1939]" lastPageId="4" lastPageNumber="175" pageId="3" pageNumber="174">
|
||
(
|
||
<figureCitation id="133561F60A37FFE6FF018643FE17F99E" box="[133,314,1631,1658]" captionStart="Fig" captionStartId="3.[125,160,1356,1378]" captionTargetBox="[146,1439,236,1296]" captionTargetId="figure-7@3.[125,1468,223,1317]" captionTargetPageId="3" captionText="Fig. 1. Euplotes amieti Dragesco, 1970 in vivo (A), after protargol (B–D) and silver nitrate (E, F) impregnation. (A) Ventral view of a rep- resentative cell. Arrows indicate caudal cirri. (B) Different shapes of macronucleus. (C, D) Ventral (C) and dorsal (D) view, showing the infraciliature and nuclear apparatus. Arrow shows the sigmoidal adoral zone. (E, F) Silverline system on ventral (E) and dorsal side (F). Arrow shows the sigmoidal adoral zone. AZM, adoral zone of membranelles; CC, caudal cirri; CVP, contractile vacuole pore; FVC, fron- toventral cirri; MC, marginal cirri; PM, paroral membrane; TC, transverse cirri. Scale bars: 100 μm." figureDoi="http://doi.org/10.5281/zenodo.12638198" httpUri="https://zenodo.org/record/12638198/files/figure.png" pageId="3" pageNumber="174">Figs 1A, C, E</figureCitation>
|
||
,
|
||
<figureCitation id="133561F60A37FFE6FEC88643FE90F99D" box="[332,445,1631,1658]" captionStart="Fig" captionStartId="4.[132,167,1231,1253]" captionTargetBox="[132,1470,223,1187]" captionTargetId="figure-170@4.[132,1470,223,1187]" captionTargetPageId="4" captionText="Fig. 2. Photomicrographs of Euplotes amieti Dragesco, 1970 in vivo (A–H), after protargol (I–K) and silver nitrate (L, M) impregnation. (A) Ventral view of a representative individual. Arrow points to the contractile vacuole. (B, C) Different body shapes. (D) Lateral view showing flattened body. (E) Posterior part of an individual, arrowheads show the caudal cirri and arrows indicate the left marginal cirri. (F) Numerous irregular ellipsoid to oval granules (possibly mitochondria) extremely densely packed beneath dorsal and ventral pellicle. (G) Detailed view, arrows point to the basal positions of dorsal cilia. (H) Showing the endoplasm and the typical 3-shaped macronucleus. (I) Individual with three caudal cirri (arrowheads). (J, K) Ventral (J) and dorsal (K) view of the same specimen, showing the infraciliature and nuclear apparatus. (L, M) Portion of dorsal (L) and ventral (M) silverline system. Arrow indicates the position of contractile vacuole pore. Scale bars: 100 μm." figureDoi="http://doi.org/10.5281/zenodo.12638200" httpUri="https://zenodo.org/record/12638200/files/figure.png" pageId="3" pageNumber="174">2A, J, K</figureCitation>
|
||
). Paroral membrane about 20 μm × 4 μm, composed of many irregularly arranged kinetosomes, positioned below buccal lip, and extending right to the proximal end of the adoral zone (
|
||
<figureCitation id="133561F60A37FFE6FD2686DBFDD9F905" box="[674,756,1735,1761]" captionStart="Fig" captionStartId="4.[132,167,1231,1253]" captionTargetBox="[132,1470,223,1187]" captionTargetId="figure-170@4.[132,1470,223,1187]" captionTargetPageId="4" captionText="Fig. 2. Photomicrographs of Euplotes amieti Dragesco, 1970 in vivo (A–H), after protargol (I–K) and silver nitrate (L, M) impregnation. (A) Ventral view of a representative individual. Arrow points to the contractile vacuole. (B, C) Different body shapes. (D) Lateral view showing flattened body. (E) Posterior part of an individual, arrowheads show the caudal cirri and arrows indicate the left marginal cirri. (F) Numerous irregular ellipsoid to oval granules (possibly mitochondria) extremely densely packed beneath dorsal and ventral pellicle. (G) Detailed view, arrows point to the basal positions of dorsal cilia. (H) Showing the endoplasm and the typical 3-shaped macronucleus. (I) Individual with three caudal cirri (arrowheads). (J, K) Ventral (J) and dorsal (K) view of the same specimen, showing the infraciliature and nuclear apparatus. (L, M) Portion of dorsal (L) and ventral (M) silverline system. Arrow indicates the position of contractile vacuole pore. Scale bars: 100 μm." figureDoi="http://doi.org/10.5281/zenodo.12638200" httpUri="https://zenodo.org/record/12638200/files/figure.png" pageId="3" pageNumber="174">Fig. 2J</figureCitation>
|
||
); cilia of paroral membrane about 6 μm long. Consistently nine frontoventral cirri (ca. 35 μm long), five transverse cirri (ca. 45 μm long), and two left marginal cirri (ca. 35 μm long) (
|
||
<figureCitation id="133561F60A37FFE6FE03874EFECBF888" box="[391,486,1874,1900]" captionStart="Fig" captionStartId="3.[125,160,1356,1378]" captionTargetBox="[146,1439,236,1296]" captionTargetId="figure-7@3.[125,1468,223,1317]" captionTargetPageId="3" captionText="Fig. 1. Euplotes amieti Dragesco, 1970 in vivo (A), after protargol (B–D) and silver nitrate (E, F) impregnation. (A) Ventral view of a rep- resentative cell. Arrows indicate caudal cirri. (B) Different shapes of macronucleus. (C, D) Ventral (C) and dorsal (D) view, showing the infraciliature and nuclear apparatus. Arrow shows the sigmoidal adoral zone. (E, F) Silverline system on ventral (E) and dorsal side (F). Arrow shows the sigmoidal adoral zone. AZM, adoral zone of membranelles; CC, caudal cirri; CVP, contractile vacuole pore; FVC, fron- toventral cirri; MC, marginal cirri; PM, paroral membrane; TC, transverse cirri. Scale bars: 100 μm." figureDoi="http://doi.org/10.5281/zenodo.12638198" httpUri="https://zenodo.org/record/12638198/files/figure.png" pageId="3" pageNumber="174">Figs 1C</figureCitation>
|
||
,
|
||
<figureCitation id="133561F60A37FFE6FE70874EFD3CF888" box="[500,529,1874,1900]" captionStart="Fig" captionStartId="4.[132,167,1231,1253]" captionTargetBox="[132,1470,223,1187]" captionTargetId="figure-170@4.[132,1470,223,1187]" captionTargetPageId="4" captionText="Fig. 2. Photomicrographs of Euplotes amieti Dragesco, 1970 in vivo (A–H), after protargol (I–K) and silver nitrate (L, M) impregnation. (A) Ventral view of a representative individual. Arrow points to the contractile vacuole. (B, C) Different body shapes. (D) Lateral view showing flattened body. (E) Posterior part of an individual, arrowheads show the caudal cirri and arrows indicate the left marginal cirri. (F) Numerous irregular ellipsoid to oval granules (possibly mitochondria) extremely densely packed beneath dorsal and ventral pellicle. (G) Detailed view, arrows point to the basal positions of dorsal cilia. (H) Showing the endoplasm and the typical 3-shaped macronucleus. (I) Individual with three caudal cirri (arrowheads). (J, K) Ventral (J) and dorsal (K) view of the same specimen, showing the infraciliature and nuclear apparatus. (L, M) Portion of dorsal (L) and ventral (M) silverline system. Arrow indicates the position of contractile vacuole pore. Scale bars: 100 μm." figureDoi="http://doi.org/10.5281/zenodo.12638200" httpUri="https://zenodo.org/record/12638200/files/figure.png" pageId="3" pageNumber="174">2J</figureCitation>
|
||
); usually two caudal cirri, occasionally three, ca. 30 μm long (
|
||
<figureCitation id="133561F60A37FFE6FDD18769FD8BF86B" box="[597,678,1909,1935]" captionStart="Fig" captionStartId="4.[132,167,1231,1253]" captionTargetBox="[132,1470,223,1187]" captionTargetId="figure-170@4.[132,1470,223,1187]" captionTargetPageId="4" captionText="Fig. 2. Photomicrographs of Euplotes amieti Dragesco, 1970 in vivo (A–H), after protargol (I–K) and silver nitrate (L, M) impregnation. (A) Ventral view of a representative individual. Arrow points to the contractile vacuole. (B, C) Different body shapes. (D) Lateral view showing flattened body. (E) Posterior part of an individual, arrowheads show the caudal cirri and arrows indicate the left marginal cirri. (F) Numerous irregular ellipsoid to oval granules (possibly mitochondria) extremely densely packed beneath dorsal and ventral pellicle. (G) Detailed view, arrows point to the basal positions of dorsal cilia. (H) Showing the endoplasm and the typical 3-shaped macronucleus. (I) Individual with three caudal cirri (arrowheads). (J, K) Ventral (J) and dorsal (K) view of the same specimen, showing the infraciliature and nuclear apparatus. (L, M) Portion of dorsal (L) and ventral (M) silverline system. Arrow indicates the position of contractile vacuole pore. Scale bars: 100 μm." figureDoi="http://doi.org/10.5281/zenodo.12638200" httpUri="https://zenodo.org/record/12638200/files/figure.png" pageId="3" pageNumber="174">Fig. 2I</figureCitation>
|
||
). 12–14 (usually 13) dorsal kineties with dikinetids extending almost entire length of body (four out of
|
||
<specimenCount id="9D08B6FA0A37FFE6FA94869EFA82F978" box="[1296,1455,1666,1692]" count="39" pageId="3" pageNumber="174" type="generic">39 specimens</specimenCount>
|
||
analyzed possessed 12 dorsal kineties and another four had 14). Usually 10–12 kinety rows located on dorsal side. Mid-dorsal kinety with 21–24 dikinetids (
|
||
<figureCitation id="133561F60A37FFE6FA0486F6FC62F8C3" captionStart="Fig" captionStartId="3.[125,160,1356,1378]" captionTargetBox="[146,1439,236,1296]" captionTargetId="figure-7@3.[125,1468,223,1317]" captionTargetPageId="3" captionText="Fig. 1. Euplotes amieti Dragesco, 1970 in vivo (A), after protargol (B–D) and silver nitrate (E, F) impregnation. (A) Ventral view of a rep- resentative cell. Arrows indicate caudal cirri. (B) Different shapes of macronucleus. (C, D) Ventral (C) and dorsal (D) view, showing the infraciliature and nuclear apparatus. Arrow shows the sigmoidal adoral zone. (E, F) Silverline system on ventral (E) and dorsal side (F). Arrow shows the sigmoidal adoral zone. AZM, adoral zone of membranelles; CC, caudal cirri; CVP, contractile vacuole pore; FVC, fron- toventral cirri; MC, marginal cirri; PM, paroral membrane; TC, transverse cirri. Scale bars: 100 μm." figureDoi="http://doi.org/10.5281/zenodo.12638198" httpUri="https://zenodo.org/record/12638198/files/figure.png" pageId="3" pageNumber="174">Fig. 1D</figureCitation>
|
||
). Macronucleus 3-shaped, with many small spheri- cal nucleoli (
|
||
<figureCitation id="133561F60A37FFE6FC468733FB09F8AD" box="[962,1060,1839,1865]" captionStart="Fig" captionStartId="3.[125,160,1356,1378]" captionTargetBox="[146,1439,236,1296]" captionTargetId="figure-7@3.[125,1468,223,1317]" captionTargetPageId="3" captionText="Fig. 1. Euplotes amieti Dragesco, 1970 in vivo (A), after protargol (B–D) and silver nitrate (E, F) impregnation. (A) Ventral view of a rep- resentative cell. Arrows indicate caudal cirri. (B) Different shapes of macronucleus. (C, D) Ventral (C) and dorsal (D) view, showing the infraciliature and nuclear apparatus. Arrow shows the sigmoidal adoral zone. (E, F) Silverline system on ventral (E) and dorsal side (F). Arrow shows the sigmoidal adoral zone. AZM, adoral zone of membranelles; CC, caudal cirri; CVP, contractile vacuole pore; FVC, fron- toventral cirri; MC, marginal cirri; PM, paroral membrane; TC, transverse cirri. Scale bars: 100 μm." figureDoi="http://doi.org/10.5281/zenodo.12638198" httpUri="https://zenodo.org/record/12638198/files/figure.png" pageId="3" pageNumber="174">Figs 1D</figureCitation>
|
||
,
|
||
<figureCitation id="133561F60A37FFE6FBB68733FB59F8AD" box="[1074,1140,1839,1865]" captionStart="Fig" captionStartId="4.[132,167,1231,1253]" captionTargetBox="[132,1470,223,1187]" captionTargetId="figure-170@4.[132,1470,223,1187]" captionTargetPageId="4" captionText="Fig. 2. Photomicrographs of Euplotes amieti Dragesco, 1970 in vivo (A–H), after protargol (I–K) and silver nitrate (L, M) impregnation. (A) Ventral view of a representative individual. Arrow points to the contractile vacuole. (B, C) Different body shapes. (D) Lateral view showing flattened body. (E) Posterior part of an individual, arrowheads show the caudal cirri and arrows indicate the left marginal cirri. (F) Numerous irregular ellipsoid to oval granules (possibly mitochondria) extremely densely packed beneath dorsal and ventral pellicle. (G) Detailed view, arrows point to the basal positions of dorsal cilia. (H) Showing the endoplasm and the typical 3-shaped macronucleus. (I) Individual with three caudal cirri (arrowheads). (J, K) Ventral (J) and dorsal (K) view of the same specimen, showing the infraciliature and nuclear apparatus. (L, M) Portion of dorsal (L) and ventral (M) silverline system. Arrow indicates the position of contractile vacuole pore. Scale bars: 100 μm." figureDoi="http://doi.org/10.5281/zenodo.12638200" httpUri="https://zenodo.org/record/12638200/files/figure.png" pageId="3" pageNumber="174">2J, K</figureCitation>
|
||
); one micronucleus, round to oval, located in the anterior part of the body near the left side, generally set in a distinct depression of the macronucleus. Silverline system on dorsal side of the double-
|
||
<taxonomicName id="4C0E06F00A30FFE1FEB58678FE9AF999" authorityName="Wrzesniowski" authorityYear="1870" box="[305,439,1636,1661]" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="4" pageNumber="175" phylum="Ciliophora" rank="species" species="eurystomus">
|
||
<emphasis id="B97AA1610A30FFE1FEB58678FE9AF999" box="[305,439,1636,1661]" italics="true" pageId="4" pageNumber="175">eurystomus</emphasis>
|
||
</taxonomicName>
|
||
<typeStatus id="54B5C3D10A30FFE1FE468678FED8F99A" box="[450,501,1636,1662]" pageId="4" pageNumber="175">type</typeStatus>
|
||
(
|
||
<figureCitation id="133561F60A30FFE1FD8D8678FDA0F99A" box="[521,653,1636,1662]" captionStart="Fig" captionStartId="3.[125,160,1356,1378]" captionTargetBox="[146,1439,236,1296]" captionTargetId="figure-7@3.[125,1468,223,1317]" captionTargetPageId="3" captionText="Fig. 1. Euplotes amieti Dragesco, 1970 in vivo (A), after protargol (B–D) and silver nitrate (E, F) impregnation. (A) Ventral view of a rep- resentative cell. Arrows indicate caudal cirri. (B) Different shapes of macronucleus. (C, D) Ventral (C) and dorsal (D) view, showing the infraciliature and nuclear apparatus. Arrow shows the sigmoidal adoral zone. (E, F) Silverline system on ventral (E) and dorsal side (F). Arrow shows the sigmoidal adoral zone. AZM, adoral zone of membranelles; CC, caudal cirri; CVP, contractile vacuole pore; FVC, fron- toventral cirri; MC, marginal cirri; PM, paroral membrane; TC, transverse cirri. Scale bars: 100 μm." figureDoi="http://doi.org/10.5281/zenodo.12638198" httpUri="https://zenodo.org/record/12638198/files/figure.png" pageId="4" pageNumber="175">Figs 1E, F</figureCitation>
|
||
,
|
||
<figureCitation id="133561F60A30FFE1FD198678FDECF99A" box="[669,705,1636,1662]" captionStart="Fig" captionStartId="4.[132,167,1231,1253]" captionTargetBox="[132,1470,223,1187]" captionTargetId="figure-170@4.[132,1470,223,1187]" captionTargetPageId="4" captionText="Fig. 2. Photomicrographs of Euplotes amieti Dragesco, 1970 in vivo (A–H), after protargol (I–K) and silver nitrate (L, M) impregnation. (A) Ventral view of a representative individual. Arrow points to the contractile vacuole. (B, C) Different body shapes. (D) Lateral view showing flattened body. (E) Posterior part of an individual, arrowheads show the caudal cirri and arrows indicate the left marginal cirri. (F) Numerous irregular ellipsoid to oval granules (possibly mitochondria) extremely densely packed beneath dorsal and ventral pellicle. (G) Detailed view, arrows point to the basal positions of dorsal cilia. (H) Showing the endoplasm and the typical 3-shaped macronucleus. (I) Individual with three caudal cirri (arrowheads). (J, K) Ventral (J) and dorsal (K) view of the same specimen, showing the infraciliature and nuclear apparatus. (L, M) Portion of dorsal (L) and ventral (M) silverline system. Arrow indicates the position of contractile vacuole pore. Scale bars: 100 μm." figureDoi="http://doi.org/10.5281/zenodo.12638200" httpUri="https://zenodo.org/record/12638200/files/figure.png" pageId="4" pageNumber="175">2L</figureCitation>
|
||
). One contractile vacuole pore positioned in the right part of the transverse cirri on ventral side (
|
||
<figureCitation id="133561F60A30FFE1FDA786B5FD50F927" box="[547,637,1705,1731]" captionStart="Fig" captionStartId="3.[125,160,1356,1378]" captionTargetBox="[146,1439,236,1296]" captionTargetId="figure-7@3.[125,1468,223,1317]" captionTargetPageId="3" captionText="Fig. 1. Euplotes amieti Dragesco, 1970 in vivo (A), after protargol (B–D) and silver nitrate (E, F) impregnation. (A) Ventral view of a rep- resentative cell. Arrows indicate caudal cirri. (B) Different shapes of macronucleus. (C, D) Ventral (C) and dorsal (D) view, showing the infraciliature and nuclear apparatus. Arrow shows the sigmoidal adoral zone. (E, F) Silverline system on ventral (E) and dorsal side (F). Arrow shows the sigmoidal adoral zone. AZM, adoral zone of membranelles; CC, caudal cirri; CVP, contractile vacuole pore; FVC, fron- toventral cirri; MC, marginal cirri; PM, paroral membrane; TC, transverse cirri. Scale bars: 100 μm." figureDoi="http://doi.org/10.5281/zenodo.12638198" httpUri="https://zenodo.org/record/12638198/files/figure.png" pageId="4" pageNumber="175">Fig. 1E</figureCitation>
|
||
).
|
||
</paragraph>
|
||
<caption id="DF712DFB0A30FFE1FF0084D3FE57FA4D" ID-DOI="http://doi.org/10.5281/zenodo.12638200" ID-Zenodo-Dep="12638200" httpUri="https://zenodo.org/record/12638200/files/figure.png" pageId="4" pageNumber="175" startId="4.[132,167,1231,1253]" targetBox="[132,1470,223,1187]" targetPageId="4" targetType="figure">
|
||
<paragraph id="8BB17D730A30FFE1FF0084D3FE57FA4D" blockId="4.[132,1464,1231,1449]" pageId="4" pageNumber="175">
|
||
<emphasis id="B97AA1610A30FFE1FF0084D3FFE8FB00" bold="true" box="[132,197,1231,1253]" pageId="4" pageNumber="175">Fig. 2.</emphasis>
|
||
Photomicrographs of
|
||
<taxonomicName id="4C0E06F00A30FFE1FE1A84D3FDF9FB00" authority="Dragesco, 1970" authorityName="Dragesco" authorityYear="1970" box="[414,724,1231,1253]" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="4" pageNumber="175" phylum="Ciliophora" rank="species" species="amieti">
|
||
<emphasis id="B97AA1610A30FFE1FE1A84D3FD18FB01" box="[414,565,1231,1253]" italics="true" pageId="4" pageNumber="175">Euplotes amieti</emphasis>
|
||
<bibRefCitation id="EF9F00820A30FFE1FDB884CCFDF9FB00" author="Dragesco J." box="[572,724,1231,1253]" pageId="4" pageNumber="175" pagination="1 - 141" refId="ref7059" refString="Dragesco J. (1970) Cilies libres du Cameroun. Ann. Fac. Sci. Jaounde (Hors serie), 1 - 141" type="book chapter" year="1970">Dragesco, 1970</bibRefCitation>
|
||
</taxonomicName>
|
||
<emphasis id="B97AA1610A30FFE1FD5F84CCFC30FB01" box="[731,797,1232,1253]" italics="true" pageId="4" pageNumber="175">in vivo</emphasis>
|
||
(A–H), after protargol (I–K) and silver nitrate (L, M) impregnation. (
|
||
<emphasis id="B97AA1610A30FFE1FF0884F7FFB0FAE4" bold="true" box="[140,157,1259,1280]" pageId="4" pageNumber="175">A</emphasis>
|
||
) Ventral view of a representative individual. Arrow points to the contractile vacuole. (
|
||
<emphasis id="B97AA1610A30FFE1FC7484F7FB32FAE5" bold="true" box="[1008,1055,1259,1281]" pageId="4" pageNumber="175">B, C</emphasis>
|
||
) Different body shapes. (
|
||
<emphasis id="B97AA1610A30FFE1FA9F84F7FA01FAE4" bold="true" box="[1307,1324,1259,1280]" pageId="4" pageNumber="175">D</emphasis>
|
||
) Lateral view showing flattened body. (
|
||
<emphasis id="B97AA1610A30FFE1FEFF851BFEA6FAF8" bold="true" box="[379,395,1287,1308]" pageId="4" pageNumber="175">E</emphasis>
|
||
) Posterior part of an individual, arrowheads show the caudal cirri and arrows indicate the left marginal cirri. (
|
||
<emphasis id="B97AA1610A30FFE1FF08853FFFB6FADC" bold="true" box="[140,155,1315,1336]" pageId="4" pageNumber="175">F</emphasis>
|
||
) Numerous irregular ellipsoid to oval granules (possibly mitochondria) extremely densely packed beneath dorsal and ventral pellicle. (
|
||
<emphasis id="B97AA1610A30FFE1FF088523FFB2FAB1" bold="true" box="[140,159,1343,1365]" pageId="4" pageNumber="175">G</emphasis>
|
||
) Detailed view, arrows point to the basal positions of dorsal cilia. (
|
||
<emphasis id="B97AA1610A30FFE1FCAE8523FC10FAB0" bold="true" box="[810,829,1343,1364]" pageId="4" pageNumber="175">H</emphasis>
|
||
) Showing the endoplasm and the typical 3-shaped macronucleus. (
|
||
<emphasis id="B97AA1610A30FFE1FF088547FFB8FA94" bold="true" box="[140,149,1371,1392]" pageId="4" pageNumber="175">I</emphasis>
|
||
) Individual with three caudal cirri (arrowheads). (
|
||
<emphasis id="B97AA1610A30FFE1FDFE8547FD88FA94" bold="true" box="[634,677,1371,1392]" pageId="4" pageNumber="175">J, K</emphasis>
|
||
) Ventral (J) and dorsal (K) view of the same specimen, showing the infraciliature and nuclear apparatus. (
|
||
<emphasis id="B97AA1610A30FFE1FEE8856BFE8DFA68" bold="true" box="[364,416,1399,1420]" pageId="4" pageNumber="175">L, M</emphasis>
|
||
) Portion of dorsal (L) and ventral (M) silverline system. Arrow indicates the position of contractile vacuole pore. Scale bars: 100 μm.
|
||
</paragraph>
|
||
</caption>
|
||
<paragraph id="8BB17D730A30FFE1FF2E86D7FA7AF99A" blockId="4.[132,780,1601,1939]" lastBlockId="4.[816,1464,1601,1940]" pageId="4" pageNumber="175">
|
||
<emphasis id="B97AA1610A30FFE1FF2E86D7FD3EF901" bold="true" box="[170,531,1739,1766]" pageId="4" pageNumber="175">
|
||
Morphogenesis (
|
||
<figureCitation id="133561F60A30FFE1FEEB86D7FE95F901" box="[367,440,1739,1765]" captionStart="Fig" captionStartId="5.[125,160,1485,1507]" captionTargetBox="[137,1433,240,1427]" captionTargetId="figure-7@5.[122,1454,225,1444]" captionTargetPageId="5" captionText="Fig. 3. Morphogenesis of Euplotes amieti after protargol impregnation. (A, B) Ventral (A) and dorsal (B) view of the same specimen at an early stage to show the five frontal-ventral-transverse cirral streaks and oral primordium within which membranelles are forming (arrow). (C, D) Ventral (C) and dorsal (D) view of the same specimen at a middle stage to show the completion of the cirral formation, the differen- tiation of caudal cirri at posterior ends of the two rightmost dorsal anlagen (arrowheads), the de novo formation of the new marginal cirri and the frontal cirrus I/1 in both proter and opisthe (arrows). (E, F) Ventral (E) and dorsal (F) view, showing the migration of newly formed cirri and the development of dorsal kineties, arrowheads show the marginal cirri. (G, H) Ventral (G) and dorsal (H) side of the same divider at a late stage showing infraciliature and nuclear apparatus. Scale bars:A–D = 100 μm; E–H = 150 μm." figureDoi="http://doi.org/10.5281/zenodo.12638202" httpUri="https://zenodo.org/record/12638202/files/figure.png" pageId="4" pageNumber="175">Figs 3</figureCitation>
|
||
and
|
||
<figureCitation id="133561F60A30FFE1FE7686D7FD2EF901" box="[498,515,1739,1765]" captionStart="Fig" captionStartId="6.[132,167,1026,1048]" captionTargetBox="[141,1470,225,980]" captionTargetId="figure-7@6.[132,1470,225,980]" captionTargetPageId="6" captionText="Fig. 4. Photomicrographs of Euplotes amieti during morphogenesis after protargol impregnation. (A) Ventral view of a middle divider showing the migration of cirri and the division of the macronucleus. (B, C) Ventral view of an early divider demonstrating two sets of frontal-ventral-transverse cirral streaks (arrowheads) and the oral primordium in opisthe (arrow). (D) To show new cirri derived from the frontal-ventral-transverse cirral anlagen. (E) Ventral view, arrows point to the paroral membrane (PM) in the proter and the development of the PM-anlage in the opisthe; arrowhead indicates the frontal cirrus I/1 in the opisthe formed de novo. (F) Ventral view, indicating the marginal anlagen of the proter (arrow) and the opisthe (arrowhead). (G) Arrowheads showing the dorsal kinety anlage of an early divider. (H) Portion of the ventral view, showing the marginal cirri in the proter (arrow) and the opisthe (arrowhead). (I) Dorsal view, arrowheads indicating the development of the dorsal kinety anlage. (J) Portion of the dorsal view, to show the newly formed caudal cirri in the proter (arrowheads). Scale bars: 100 μm." figureDoi="http://doi.org/10.5281/zenodo.12638204" httpUri="https://zenodo.org/record/12638204/files/figure.png" pageId="4" pageNumber="175">4</figureCitation>
|
||
):
|
||
</emphasis>
|
||
Stomatogenesis starts with the appearance of a small patch of kinetosomes, which is the opisthe’s oral primordium, within a pouch beneath the cortex of the ventral surface, positioned between the cytostome and the left marginal cirri (
|
||
<figureCitation id="133561F60A30FFE1FDEE874AFDE6F895" box="[618,715,1878,1905]" captionStart="Fig" captionStartId="5.[125,160,1485,1507]" captionTargetBox="[137,1433,240,1427]" captionTargetId="figure-7@5.[122,1454,225,1444]" captionTargetPageId="5" captionText="Fig. 3. Morphogenesis of Euplotes amieti after protargol impregnation. (A, B) Ventral (A) and dorsal (B) view of the same specimen at an early stage to show the five frontal-ventral-transverse cirral streaks and oral primordium within which membranelles are forming (arrow). (C, D) Ventral (C) and dorsal (D) view of the same specimen at a middle stage to show the completion of the cirral formation, the differen- tiation of caudal cirri at posterior ends of the two rightmost dorsal anlagen (arrowheads), the de novo formation of the new marginal cirri and the frontal cirrus I/1 in both proter and opisthe (arrows). (E, F) Ventral (E) and dorsal (F) view, showing the migration of newly formed cirri and the development of dorsal kineties, arrowheads show the marginal cirri. (G, H) Ventral (G) and dorsal (H) side of the same divider at a late stage showing infraciliature and nuclear apparatus. Scale bars:A–D = 100 μm; E–H = 150 μm." figureDoi="http://doi.org/10.5281/zenodo.12638202" httpUri="https://zenodo.org/record/12638202/files/figure.png" pageId="4" pageNumber="175">Figs 3A</figureCitation>
|
||
,
|
||
<figureCitation id="133561F60A30FFE1FD5D874BFDD3F895" box="[729,766,1879,1905]" captionStart="Fig" captionStartId="6.[132,167,1026,1048]" captionTargetBox="[141,1470,225,980]" captionTargetId="figure-7@6.[132,1470,225,980]" captionTargetPageId="6" captionText="Fig. 4. Photomicrographs of Euplotes amieti during morphogenesis after protargol impregnation. (A) Ventral view of a middle divider showing the migration of cirri and the division of the macronucleus. (B, C) Ventral view of an early divider demonstrating two sets of frontal-ventral-transverse cirral streaks (arrowheads) and the oral primordium in opisthe (arrow). (D) To show new cirri derived from the frontal-ventral-transverse cirral anlagen. (E) Ventral view, arrows point to the paroral membrane (PM) in the proter and the development of the PM-anlage in the opisthe; arrowhead indicates the frontal cirrus I/1 in the opisthe formed de novo. (F) Ventral view, indicating the marginal anlagen of the proter (arrow) and the opisthe (arrowhead). (G) Arrowheads showing the dorsal kinety anlage of an early divider. (H) Portion of the ventral view, showing the marginal cirri in the proter (arrow) and the opisthe (arrowhead). (I) Dorsal view, arrowheads indicating the development of the dorsal kinety anlage. (J) Portion of the dorsal view, to show the newly formed caudal cirri in the proter (arrowheads). Scale bars: 100 μm." figureDoi="http://doi.org/10.5281/zenodo.12638204" httpUri="https://zenodo.org/record/12638204/files/figure.png" pageId="4" pageNumber="175">4C</figureCitation>
|
||
). As the pouch enlarges, kinetosomes proliferate rapidly and begin to align into membranelles from the anterior end and organize towards the posterior (
|
||
<figureCitation id="133561F60A30FFE1FB448678FA31F99A" box="[1216,1308,1636,1662]" captionStart="Fig" captionStartId="5.[125,160,1485,1507]" captionTargetBox="[137,1433,240,1427]" captionTargetId="figure-7@5.[122,1454,225,1444]" captionTargetPageId="5" captionText="Fig. 3. Morphogenesis of Euplotes amieti after protargol impregnation. (A, B) Ventral (A) and dorsal (B) view of the same specimen at an early stage to show the five frontal-ventral-transverse cirral streaks and oral primordium within which membranelles are forming (arrow). (C, D) Ventral (C) and dorsal (D) view of the same specimen at a middle stage to show the completion of the cirral formation, the differen- tiation of caudal cirri at posterior ends of the two rightmost dorsal anlagen (arrowheads), the de novo formation of the new marginal cirri and the frontal cirrus I/1 in both proter and opisthe (arrows). (E, F) Ventral (E) and dorsal (F) view, showing the migration of newly formed cirri and the development of dorsal kineties, arrowheads show the marginal cirri. (G, H) Ventral (G) and dorsal (H) side of the same divider at a late stage showing infraciliature and nuclear apparatus. Scale bars:A–D = 100 μm; E–H = 150 μm." figureDoi="http://doi.org/10.5281/zenodo.12638202" httpUri="https://zenodo.org/record/12638202/files/figure.png" pageId="4" pageNumber="175">Figs 3C</figureCitation>
|
||
,
|
||
<figureCitation id="133561F60A30FFE1FAA38678FA66F99A" box="[1319,1355,1636,1662]" captionStart="Fig" captionStartId="6.[132,167,1026,1048]" captionTargetBox="[141,1470,225,980]" captionTargetId="figure-7@6.[132,1470,225,980]" captionTargetPageId="6" captionText="Fig. 4. Photomicrographs of Euplotes amieti during morphogenesis after protargol impregnation. (A) Ventral view of a middle divider showing the migration of cirri and the division of the macronucleus. (B, C) Ventral view of an early divider demonstrating two sets of frontal-ventral-transverse cirral streaks (arrowheads) and the oral primordium in opisthe (arrow). (D) To show new cirri derived from the frontal-ventral-transverse cirral anlagen. (E) Ventral view, arrows point to the paroral membrane (PM) in the proter and the development of the PM-anlage in the opisthe; arrowhead indicates the frontal cirrus I/1 in the opisthe formed de novo. (F) Ventral view, indicating the marginal anlagen of the proter (arrow) and the opisthe (arrowhead). (G) Arrowheads showing the dorsal kinety anlage of an early divider. (H) Portion of the ventral view, showing the marginal cirri in the proter (arrow) and the opisthe (arrowhead). (I) Dorsal view, arrowheads indicating the development of the dorsal kinety anlage. (J) Portion of the dorsal view, to show the newly formed caudal cirri in the proter (arrowheads). Scale bars: 100 μm." figureDoi="http://doi.org/10.5281/zenodo.12638204" httpUri="https://zenodo.org/record/12638204/files/figure.png" pageId="4" pageNumber="175">4E</figureCitation>
|
||
).
|
||
</paragraph>
|
||
<paragraph id="8BB17D730A30FFE1FCD2869BFBB2F8ED" blockId="4.[816,1464,1601,1940]" pageId="4" pageNumber="175">
|
||
An additional anlage, the primordium for the paroral membrane (UM-anlage), appears within the subcortical pouch, which is located close to the posterior end of the oral primordium (
|
||
<figureCitation id="133561F60A30FFE1FB8586F2FB4CF8ED" box="[1025,1121,1774,1801]" captionStart="Fig" captionStartId="5.[125,160,1485,1507]" captionTargetBox="[137,1433,240,1427]" captionTargetId="figure-7@5.[122,1454,225,1444]" captionTargetPageId="5" captionText="Fig. 3. Morphogenesis of Euplotes amieti after protargol impregnation. (A, B) Ventral (A) and dorsal (B) view of the same specimen at an early stage to show the five frontal-ventral-transverse cirral streaks and oral primordium within which membranelles are forming (arrow). (C, D) Ventral (C) and dorsal (D) view of the same specimen at a middle stage to show the completion of the cirral formation, the differen- tiation of caudal cirri at posterior ends of the two rightmost dorsal anlagen (arrowheads), the de novo formation of the new marginal cirri and the frontal cirrus I/1 in both proter and opisthe (arrows). (E, F) Ventral (E) and dorsal (F) view, showing the migration of newly formed cirri and the development of dorsal kineties, arrowheads show the marginal cirri. (G, H) Ventral (G) and dorsal (H) side of the same divider at a late stage showing infraciliature and nuclear apparatus. Scale bars:A–D = 100 μm; E–H = 150 μm." figureDoi="http://doi.org/10.5281/zenodo.12638202" httpUri="https://zenodo.org/record/12638202/files/figure.png" pageId="4" pageNumber="175">Figs 3C</figureCitation>
|
||
,
|
||
<figureCitation id="133561F60A30FFE1FBE986F3FBBEF8ED" box="[1133,1171,1775,1801]" captionStart="Fig" captionStartId="6.[132,167,1026,1048]" captionTargetBox="[141,1470,225,980]" captionTargetId="figure-7@6.[132,1470,225,980]" captionTargetPageId="6" captionText="Fig. 4. Photomicrographs of Euplotes amieti during morphogenesis after protargol impregnation. (A) Ventral view of a middle divider showing the migration of cirri and the division of the macronucleus. (B, C) Ventral view of an early divider demonstrating two sets of frontal-ventral-transverse cirral streaks (arrowheads) and the oral primordium in opisthe (arrow). (D) To show new cirri derived from the frontal-ventral-transverse cirral anlagen. (E) Ventral view, arrows point to the paroral membrane (PM) in the proter and the development of the PM-anlage in the opisthe; arrowhead indicates the frontal cirrus I/1 in the opisthe formed de novo. (F) Ventral view, indicating the marginal anlagen of the proter (arrow) and the opisthe (arrowhead). (G) Arrowheads showing the dorsal kinety anlage of an early divider. (H) Portion of the ventral view, showing the marginal cirri in the proter (arrow) and the opisthe (arrowhead). (I) Dorsal view, arrowheads indicating the development of the dorsal kinety anlage. (J) Portion of the dorsal view, to show the newly formed caudal cirri in the proter (arrowheads). Scale bars: 100 μm." figureDoi="http://doi.org/10.5281/zenodo.12638204" httpUri="https://zenodo.org/record/12638204/files/figure.png" pageId="4" pageNumber="175">4E</figureCitation>
|
||
).
|
||
</paragraph>
|
||
<paragraph id="8BB17D730A30FFE1FCD2870DFB6DF877" blockId="4.[816,1464,1601,1940]" pageId="4" pageNumber="175">
|
||
Following this, the UM-anlage begins to lengthen and becomes broader. Throughout the entire morphogenetic process, the parental adoral zone remains nearly intact (
|
||
<figureCitation id="133561F60A30FFE1FC058765FB2CF870" box="[897,1025,1913,1940]" captionStart="Fig" captionStartId="5.[125,160,1485,1507]" captionTargetBox="[137,1433,240,1427]" captionTargetId="figure-7@5.[122,1454,225,1444]" captionTargetPageId="5" captionText="Fig. 3. Morphogenesis of Euplotes amieti after protargol impregnation. (A, B) Ventral (A) and dorsal (B) view of the same specimen at an early stage to show the five frontal-ventral-transverse cirral streaks and oral primordium within which membranelles are forming (arrow). (C, D) Ventral (C) and dorsal (D) view of the same specimen at a middle stage to show the completion of the cirral formation, the differen- tiation of caudal cirri at posterior ends of the two rightmost dorsal anlagen (arrowheads), the de novo formation of the new marginal cirri and the frontal cirrus I/1 in both proter and opisthe (arrows). (E, F) Ventral (E) and dorsal (F) view, showing the migration of newly formed cirri and the development of dorsal kineties, arrowheads show the marginal cirri. (G, H) Ventral (G) and dorsal (H) side of the same divider at a late stage showing infraciliature and nuclear apparatus. Scale bars:A–D = 100 μm; E–H = 150 μm." figureDoi="http://doi.org/10.5281/zenodo.12638202" httpUri="https://zenodo.org/record/12638202/files/figure.png" pageId="4" pageNumber="175">Figs 3C, E</figureCitation>
|
||
,
|
||
<figureCitation id="133561F60A30FFE1FB8A8765FB1EF877" box="[1038,1075,1913,1939]" captionStart="Fig" captionStartId="6.[132,167,1026,1048]" captionTargetBox="[141,1470,225,980]" captionTargetId="figure-7@6.[132,1470,225,980]" captionTargetPageId="6" captionText="Fig. 4. Photomicrographs of Euplotes amieti during morphogenesis after protargol impregnation. (A) Ventral view of a middle divider showing the migration of cirri and the division of the macronucleus. (B, C) Ventral view of an early divider demonstrating two sets of frontal-ventral-transverse cirral streaks (arrowheads) and the oral primordium in opisthe (arrow). (D) To show new cirri derived from the frontal-ventral-transverse cirral anlagen. (E) Ventral view, arrows point to the paroral membrane (PM) in the proter and the development of the PM-anlage in the opisthe; arrowhead indicates the frontal cirrus I/1 in the opisthe formed de novo. (F) Ventral view, indicating the marginal anlagen of the proter (arrow) and the opisthe (arrowhead). (G) Arrowheads showing the dorsal kinety anlage of an early divider. (H) Portion of the ventral view, showing the marginal cirri in the proter (arrow) and the opisthe (arrowhead). (I) Dorsal view, arrowheads indicating the development of the dorsal kinety anlage. (J) Portion of the dorsal view, to show the newly formed caudal cirri in the proter (arrowheads). Scale bars: 100 μm." figureDoi="http://doi.org/10.5281/zenodo.12638204" httpUri="https://zenodo.org/record/12638204/files/figure.png" pageId="4" pageNumber="175">4E</figureCitation>
|
||
).
|
||
</paragraph>
|
||
<caption id="DF712DFB0A31FFE0FFF985D1FB70F96F" ID-DOI="http://doi.org/10.5281/zenodo.12638202" ID-Zenodo-Dep="12638202" httpUri="https://zenodo.org/record/12638202/files/figure.png" pageId="5" pageNumber="176" startId="5.[125,160,1485,1507]" targetBox="[137,1433,240,1427]" targetPageId="5" targetType="figure">
|
||
<paragraph id="8BB17D730A31FFE0FFF985D1FB70F96F" blockId="5.[125,1456,1485,1676]" pageId="5" pageNumber="176">
|
||
<emphasis id="B97AA1610A31FFE0FFF985D1FF91FA07" bold="true" box="[125,188,1485,1507]" pageId="5" pageNumber="176">Fig. 3.</emphasis>
|
||
Morphogenesis of
|
||
<taxonomicName id="4C0E06F00A31FFE0FEF385D2FD20FA07" authorityName="Dragesco" authorityYear="1970" box="[375,525,1486,1507]" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="5" pageNumber="176" phylum="Ciliophora" rank="species" species="amieti">
|
||
<emphasis id="B97AA1610A31FFE0FEF385D2FD20FA07" box="[375,525,1486,1507]" italics="true" pageId="5" pageNumber="176">Euplotes amieti</emphasis>
|
||
</taxonomicName>
|
||
after protargol impregnation. (
|
||
<emphasis id="B97AA1610A31FFE0FCB385D1FC48FA07" bold="true" box="[823,869,1485,1507]" pageId="5" pageNumber="176">A, B</emphasis>
|
||
) Ventral (A) and dorsal (B) view of the same specimen at an early stage to show the five frontal-ventral-transverse cirral streaks and oral primordium within which membranelles are forming (arrow). (
|
||
<emphasis id="B97AA1610A31FFE0FF018619FF9EF9FF" bold="true" box="[133,179,1541,1563]" pageId="5" pageNumber="176">C, D</emphasis>
|
||
) Ventral (C) and dorsal (D) view of the same specimen at a middle stage to show the completion of the cirral formation, the differen- tiation of caudal cirri at posterior ends of the two rightmost dorsal anlagen (arrowheads), the
|
||
<emphasis id="B97AA1610A31FFE0FB82863EFB7FF9D3" box="[1030,1106,1570,1591]" italics="true" pageId="5" pageNumber="176">de novo</emphasis>
|
||
formation of the new marginal cirri and the frontal cirrus I/1 in both proter and opisthe (arrows). (
|
||
<emphasis id="B97AA1610A31FFE0FD4D8622FDD9F9B7" bold="true" box="[713,756,1598,1619]" pageId="5" pageNumber="176">E, F</emphasis>
|
||
) Ventral (E) and dorsal (F) view, showing the migration of newly formed cirri and the development of dorsal kineties, arrowheads show the marginal cirri. (
|
||
<emphasis id="B97AA1610A31FFE0FC088645FC93F98B" bold="true" box="[908,958,1625,1647]" pageId="5" pageNumber="176">G, H</emphasis>
|
||
) Ventral (G) and dorsal (H) side of the same divider at a late stage showing infraciliature and nuclear apparatus. Scale bars: A–D = 100 μm; E–H = 150 μm.
|
||
</paragraph>
|
||
</caption>
|
||
<paragraph id="8BB17D730A31FFE3FF2786F3FEA0FA12" blockId="5.[125,771,1775,1940]" lastBlockId="6.[132,779,1465,1942]" lastPageId="6" lastPageNumber="177" pageId="5" pageNumber="176">
|
||
Development of frontal-ventral-transverse cirral anlagen (FVT-anlagen): at the same time as the oral primordium is formed, two sets of FVT-anlagen develop
|
||
<emphasis id="B97AA1610A31FFE0FF49874BFE1CF895" box="[205,305,1879,1905]" italics="true" pageId="5" pageNumber="176">de novo</emphasis>
|
||
as multiple basal bodies anterior to the transverse cirri (
|
||
<figureCitation id="133561F60A31FFE0FEFE8765FECFF870" box="[378,482,1913,1940]" captionStart="Fig" captionStartId="5.[125,160,1485,1507]" captionTargetBox="[137,1433,240,1427]" captionTargetId="figure-7@5.[122,1454,225,1444]" captionTargetPageId="5" captionText="Fig. 3. Morphogenesis of Euplotes amieti after protargol impregnation. (A, B) Ventral (A) and dorsal (B) view of the same specimen at an early stage to show the five frontal-ventral-transverse cirral streaks and oral primordium within which membranelles are forming (arrow). (C, D) Ventral (C) and dorsal (D) view of the same specimen at a middle stage to show the completion of the cirral formation, the differen- tiation of caudal cirri at posterior ends of the two rightmost dorsal anlagen (arrowheads), the de novo formation of the new marginal cirri and the frontal cirrus I/1 in both proter and opisthe (arrows). (E, F) Ventral (E) and dorsal (F) view, showing the migration of newly formed cirri and the development of dorsal kineties, arrowheads show the marginal cirri. (G, H) Ventral (G) and dorsal (H) side of the same divider at a late stage showing infraciliature and nuclear apparatus. Scale bars:A–D = 100 μm; E–H = 150 μm." figureDoi="http://doi.org/10.5281/zenodo.12638202" httpUri="https://zenodo.org/record/12638202/files/figure.png" pageId="5" pageNumber="176">Figs 3A</figureCitation>
|
||
,
|
||
<figureCitation id="133561F60A31FFE0FE778766FD34F870" box="[499,537,1914,1940]" captionStart="Fig" captionStartId="6.[132,167,1026,1048]" captionTargetBox="[141,1470,225,980]" captionTargetId="figure-7@6.[132,1470,225,980]" captionTargetPageId="6" captionText="Fig. 4. Photomicrographs of Euplotes amieti during morphogenesis after protargol impregnation. (A) Ventral view of a middle divider showing the migration of cirri and the division of the macronucleus. (B, C) Ventral view of an early divider demonstrating two sets of frontal-ventral-transverse cirral streaks (arrowheads) and the oral primordium in opisthe (arrow). (D) To show new cirri derived from the frontal-ventral-transverse cirral anlagen. (E) Ventral view, arrows point to the paroral membrane (PM) in the proter and the development of the PM-anlage in the opisthe; arrowhead indicates the frontal cirrus I/1 in the opisthe formed de novo. (F) Ventral view, indicating the marginal anlagen of the proter (arrow) and the opisthe (arrowhead). (G) Arrowheads showing the dorsal kinety anlage of an early divider. (H) Portion of the ventral view, showing the marginal cirri in the proter (arrow) and the opisthe (arrowhead). (I) Dorsal view, arrowheads indicating the development of the dorsal kinety anlage. (J) Portion of the dorsal view, to show the newly formed caudal cirri in the proter (arrowheads). Scale bars: 100 μm." figureDoi="http://doi.org/10.5281/zenodo.12638204" httpUri="https://zenodo.org/record/12638204/files/figure.png" pageId="5" pageNumber="176">4B</figureCitation>
|
||
); each set consists of five streaks (anlagen II–VI). Each cirral streak ex- tends in both directions with a proliferation of kinetosomes. Obviously, no parental ciliary organelles are involved in the formation of these anlagen. The cirral streaks then broaden and break apart in a 3:3:3:2:2 pattern (
|
||
<figureCitation id="133561F60A32FFE3FF6385A5FE64FA37" box="[231,329,1465,1491]" captionStart="Fig" captionStartId="5.[125,160,1485,1507]" captionTargetBox="[137,1433,240,1427]" captionTargetId="figure-7@5.[122,1454,225,1444]" captionTargetPageId="5" captionText="Fig. 3. Morphogenesis of Euplotes amieti after protargol impregnation. (A, B) Ventral (A) and dorsal (B) view of the same specimen at an early stage to show the five frontal-ventral-transverse cirral streaks and oral primordium within which membranelles are forming (arrow). (C, D) Ventral (C) and dorsal (D) view of the same specimen at a middle stage to show the completion of the cirral formation, the differen- tiation of caudal cirri at posterior ends of the two rightmost dorsal anlagen (arrowheads), the de novo formation of the new marginal cirri and the frontal cirrus I/1 in both proter and opisthe (arrows). (E, F) Ventral (E) and dorsal (F) view, showing the migration of newly formed cirri and the development of dorsal kineties, arrowheads show the marginal cirri. (G, H) Ventral (G) and dorsal (H) side of the same divider at a late stage showing infraciliature and nuclear apparatus. Scale bars:A–D = 100 μm; E–H = 150 μm." figureDoi="http://doi.org/10.5281/zenodo.12638202" httpUri="https://zenodo.org/record/12638202/files/figure.png" pageId="6" pageNumber="177">Figs 3C</figureCitation>
|
||
,
|
||
<figureCitation id="133561F60A32FFE3FED185A5FE50FA37" box="[341,381,1465,1491]" captionStart="Fig" captionStartId="6.[132,167,1026,1048]" captionTargetBox="[141,1470,225,980]" captionTargetId="figure-7@6.[132,1470,225,980]" captionTargetPageId="6" captionText="Fig. 4. Photomicrographs of Euplotes amieti during morphogenesis after protargol impregnation. (A) Ventral view of a middle divider showing the migration of cirri and the division of the macronucleus. (B, C) Ventral view of an early divider demonstrating two sets of frontal-ventral-transverse cirral streaks (arrowheads) and the oral primordium in opisthe (arrow). (D) To show new cirri derived from the frontal-ventral-transverse cirral anlagen. (E) Ventral view, arrows point to the paroral membrane (PM) in the proter and the development of the PM-anlage in the opisthe; arrowhead indicates the frontal cirrus I/1 in the opisthe formed de novo. (F) Ventral view, indicating the marginal anlagen of the proter (arrow) and the opisthe (arrowhead). (G) Arrowheads showing the dorsal kinety anlage of an early divider. (H) Portion of the ventral view, showing the marginal cirri in the proter (arrow) and the opisthe (arrowhead). (I) Dorsal view, arrowheads indicating the development of the dorsal kinety anlage. (J) Portion of the dorsal view, to show the newly formed caudal cirri in the proter (arrowheads). Scale bars: 100 μm." figureDoi="http://doi.org/10.5281/zenodo.12638204" httpUri="https://zenodo.org/record/12638204/files/figure.png" pageId="6" pageNumber="177">4D</figureCitation>
|
||
), migrating to develop as distinct cirri to daughter cells.
|
||
</paragraph>
|
||
<caption id="DF712DFB0A32FFE3FF00841EFEE3FB1C" ID-DOI="http://doi.org/10.5281/zenodo.12638204" ID-Zenodo-Dep="12638204" httpUri="https://zenodo.org/record/12638204/files/figure.png" pageId="6" pageNumber="177" startId="6.[132,167,1026,1048]" targetBox="[141,1470,225,980]" targetPageId="6" targetType="figure">
|
||
<paragraph id="8BB17D730A32FFE3FF00841EFEE3FB1C" blockId="6.[132,1464,1026,1272]" pageId="6" pageNumber="177">
|
||
<emphasis id="B97AA1610A32FFE3FF00841EFFEBFBF3" bold="true" box="[132,198,1026,1048]" pageId="6" pageNumber="177">Fig. 4.</emphasis>
|
||
Photomicrographs of
|
||
<taxonomicName id="4C0E06F00A32FFE3FE20841EFD11FBFC" authorityName="Dragesco" authorityYear="1970" box="[420,572,1026,1048]" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="6" pageNumber="177" phylum="Ciliophora" rank="species" species="amieti">
|
||
<emphasis id="B97AA1610A32FFE3FE20841EFD11FBFC" box="[420,572,1026,1048]" italics="true" pageId="6" pageNumber="177">Euplotes amieti</emphasis>
|
||
</taxonomicName>
|
||
during morphogenesis after protargol impregnation. (
|
||
<emphasis id="B97AA1610A32FFE3FBD5841EFB4FFBF3" bold="true" box="[1105,1122,1026,1047]" pageId="6" pageNumber="177">A</emphasis>
|
||
) Ventral view of a middle divider showing the migration of cirri and the division of the macronucleus. (
|
||
<emphasis id="B97AA1610A32FFE3FCC28403FC5BFBD0" bold="true" box="[838,886,1054,1076]" pageId="6" pageNumber="177">B, C</emphasis>
|
||
) Ventral view of an early divider demonstrating two sets of frontal-ventral-transverse cirral streaks (arrowheads) and the oral primordium in opisthe (arrow). (
|
||
<emphasis id="B97AA1610A32FFE3FBCC8427FB74FBB4" bold="true" box="[1096,1113,1083,1104]" pageId="6" pageNumber="177">D</emphasis>
|
||
) To show new cirri derived from the frontal-ventral-transverse cirral anlagen. (
|
||
<emphasis id="B97AA1610A32FFE3FD9D844BFD04FB88" bold="true" box="[537,553,1111,1132]" pageId="6" pageNumber="177">E</emphasis>
|
||
) Ventral view, arrows point to the paroral membrane (PM) in the proter and the development of the PM-anlage in the opisthe; arrowhead indicates the frontal cirrus I/1 in the opisthe formed
|
||
<emphasis id="B97AA1610A32FFE3FBAA846EFB57FB6C" box="[1070,1146,1138,1160]" italics="true" pageId="6" pageNumber="177">de novo</emphasis>
|
||
. (
|
||
<emphasis id="B97AA1610A32FFE3FB0B846FFBB3FB6C" bold="true" box="[1167,1182,1139,1160]" pageId="6" pageNumber="177">F</emphasis>
|
||
) Ventral view, indicating the marginal anlagen of the proter (arrow) and the opisthe (arrowhead). (
|
||
<emphasis id="B97AA1610A32FFE3FCA48492FC1EFB40" bold="true" box="[800,819,1166,1188]" pageId="6" pageNumber="177">G</emphasis>
|
||
) Arrowheads showing the dorsal kinety anlage of an early divider. (
|
||
<emphasis id="B97AA1610A32FFE3FF0884B7FFB2FB24" bold="true" box="[140,159,1195,1216]" pageId="6" pageNumber="177">H</emphasis>
|
||
) Portion of the ventral view, showing the marginal cirri in the proter (arrow) and the opisthe (arrowhead). (
|
||
<emphasis id="B97AA1610A32FFE3FB3484B7FB94FB24" bold="true" box="[1200,1209,1195,1216]" pageId="6" pageNumber="177">I</emphasis>
|
||
) Dorsal view, arrowheads indicating the development of the dorsal kinety anlage. (
|
||
<emphasis id="B97AA1610A32FFE3FD2384DBFD9EFB38" bold="true" box="[679,691,1223,1244]" pageId="6" pageNumber="177">J</emphasis>
|
||
) Portion of the dorsal view, to show the newly formed caudal cirri in the proter (arrowheads). Scale bars: 100 μm.
|
||
</paragraph>
|
||
</caption>
|
||
<paragraph id="8BB17D730A32FFE3FF2E85E2FEA2F872" blockId="6.[132,779,1465,1942]" pageId="6" pageNumber="177">
|
||
Immediately following the breakup of the FVTanlagen, two small patches of kinetosomes appear in the outermost region of the cortex near the original leftmost frontal cirrus and between two adoral zone membranelles (
|
||
<figureCitation id="133561F60A32FFE3FF788695FE76F947" box="[252,347,1673,1699]" captionStart="Fig" captionStartId="5.[125,160,1485,1507]" captionTargetBox="[137,1433,240,1427]" captionTargetId="figure-7@5.[122,1454,225,1444]" captionTargetPageId="5" captionText="Fig. 3. Morphogenesis of Euplotes amieti after protargol impregnation. (A, B) Ventral (A) and dorsal (B) view of the same specimen at an early stage to show the five frontal-ventral-transverse cirral streaks and oral primordium within which membranelles are forming (arrow). (C, D) Ventral (C) and dorsal (D) view of the same specimen at a middle stage to show the completion of the cirral formation, the differen- tiation of caudal cirri at posterior ends of the two rightmost dorsal anlagen (arrowheads), the de novo formation of the new marginal cirri and the frontal cirrus I/1 in both proter and opisthe (arrows). (E, F) Ventral (E) and dorsal (F) view, showing the migration of newly formed cirri and the development of dorsal kineties, arrowheads show the marginal cirri. (G, H) Ventral (G) and dorsal (H) side of the same divider at a late stage showing infraciliature and nuclear apparatus. Scale bars:A–D = 100 μm; E–H = 150 μm." figureDoi="http://doi.org/10.5281/zenodo.12638202" httpUri="https://zenodo.org/record/12638202/files/figure.png" pageId="6" pageNumber="177">Figs 3C</figureCitation>
|
||
,
|
||
<figureCitation id="133561F60A32FFE3FEE28695FEA1F947" box="[358,396,1673,1699]" captionStart="Fig" captionStartId="6.[132,167,1026,1048]" captionTargetBox="[141,1470,225,980]" captionTargetId="figure-7@6.[132,1470,225,980]" captionTargetPageId="6" captionText="Fig. 4. Photomicrographs of Euplotes amieti during morphogenesis after protargol impregnation. (A) Ventral view of a middle divider showing the migration of cirri and the division of the macronucleus. (B, C) Ventral view of an early divider demonstrating two sets of frontal-ventral-transverse cirral streaks (arrowheads) and the oral primordium in opisthe (arrow). (D) To show new cirri derived from the frontal-ventral-transverse cirral anlagen. (E) Ventral view, arrows point to the paroral membrane (PM) in the proter and the development of the PM-anlage in the opisthe; arrowhead indicates the frontal cirrus I/1 in the opisthe formed de novo. (F) Ventral view, indicating the marginal anlagen of the proter (arrow) and the opisthe (arrowhead). (G) Arrowheads showing the dorsal kinety anlage of an early divider. (H) Portion of the ventral view, showing the marginal cirri in the proter (arrow) and the opisthe (arrowhead). (I) Dorsal view, arrowheads indicating the development of the dorsal kinety anlage. (J) Portion of the dorsal view, to show the newly formed caudal cirri in the proter (arrowheads). Scale bars: 100 μm." figureDoi="http://doi.org/10.5281/zenodo.12638204" httpUri="https://zenodo.org/record/12638204/files/figure.png" pageId="6" pageNumber="177">4E</figureCitation>
|
||
). These patches form new anlage for the frontal cirrus I/1 of the proter and opisthe respectively. These two anlagen are formed
|
||
<emphasis id="B97AA1610A32FFE3FDFE86D2FDFAF90C" box="[634,727,1742,1768]" italics="true" pageId="6" pageNumber="177">de novo</emphasis>
|
||
, i.e. evidently no old cirrus or paroral membrane is involved in the formation of the anlagen. Subsequently, these two anlagen develop into the frontal cirrus I/1 by rapid proliferation of kinetosomes and migrate to their final positions (
|
||
<figureCitation id="133561F60A32FFE3FF7A8760FEACF872" box="[254,385,1916,1942]" captionStart="Fig" captionStartId="5.[125,160,1485,1507]" captionTargetBox="[137,1433,240,1427]" captionTargetId="figure-7@5.[122,1454,225,1444]" captionTargetPageId="5" captionText="Fig. 3. Morphogenesis of Euplotes amieti after protargol impregnation. (A, B) Ventral (A) and dorsal (B) view of the same specimen at an early stage to show the five frontal-ventral-transverse cirral streaks and oral primordium within which membranelles are forming (arrow). (C, D) Ventral (C) and dorsal (D) view of the same specimen at a middle stage to show the completion of the cirral formation, the differen- tiation of caudal cirri at posterior ends of the two rightmost dorsal anlagen (arrowheads), the de novo formation of the new marginal cirri and the frontal cirrus I/1 in both proter and opisthe (arrows). (E, F) Ventral (E) and dorsal (F) view, showing the migration of newly formed cirri and the development of dorsal kineties, arrowheads show the marginal cirri. (G, H) Ventral (G) and dorsal (H) side of the same divider at a late stage showing infraciliature and nuclear apparatus. Scale bars:A–D = 100 μm; E–H = 150 μm." figureDoi="http://doi.org/10.5281/zenodo.12638202" httpUri="https://zenodo.org/record/12638202/files/figure.png" pageId="6" pageNumber="177">Figs 3E, G</figureCitation>
|
||
).
|
||
</paragraph>
|
||
<paragraph id="8BB17D730A32FFE3FCD285A5FBCAF922" blockId="6.[816,1463,1465,1942]" pageId="6" pageNumber="177">
|
||
Development of marginal cirri: the anlagen of the left marginal cirri for the proter and opisthe are formed
|
||
<emphasis id="B97AA1610A32FFE3FCB485E2FCBFF9FC" box="[816,914,1534,1560]" italics="true" pageId="6" pageNumber="177">de novo</emphasis>
|
||
. One anlage for the marginal cirri forms as a short row of kinetosomes near the parental adoral zone in the proter, and the other near the newly formed adoral zone in the opisthe (
|
||
<figureCitation id="133561F60A32FFE3FBFC867AFBF9F964" box="[1144,1236,1638,1664]" captionStart="Fig" captionStartId="6.[132,167,1026,1048]" captionTargetBox="[141,1470,225,980]" captionTargetId="figure-7@6.[132,1470,225,980]" captionTargetPageId="6" captionText="Fig. 4. Photomicrographs of Euplotes amieti during morphogenesis after protargol impregnation. (A) Ventral view of a middle divider showing the migration of cirri and the division of the macronucleus. (B, C) Ventral view of an early divider demonstrating two sets of frontal-ventral-transverse cirral streaks (arrowheads) and the oral primordium in opisthe (arrow). (D) To show new cirri derived from the frontal-ventral-transverse cirral anlagen. (E) Ventral view, arrows point to the paroral membrane (PM) in the proter and the development of the PM-anlage in the opisthe; arrowhead indicates the frontal cirrus I/1 in the opisthe formed de novo. (F) Ventral view, indicating the marginal anlagen of the proter (arrow) and the opisthe (arrowhead). (G) Arrowheads showing the dorsal kinety anlage of an early divider. (H) Portion of the ventral view, showing the marginal cirri in the proter (arrow) and the opisthe (arrowhead). (I) Dorsal view, arrowheads indicating the development of the dorsal kinety anlage. (J) Portion of the dorsal view, to show the newly formed caudal cirri in the proter (arrowheads). Scale bars: 100 μm." figureDoi="http://doi.org/10.5281/zenodo.12638204" httpUri="https://zenodo.org/record/12638204/files/figure.png" pageId="6" pageNumber="177">Fig. 4F</figureCitation>
|
||
). Each anlage subsequently enlarges and segments to form the marginal cirri for daughter cells (
|
||
<figureCitation id="133561F60A32FFE3FBC186B0FB88F922" box="[1093,1189,1708,1734]" captionStart="Fig" captionStartId="5.[125,160,1485,1507]" captionTargetBox="[137,1433,240,1427]" captionTargetId="figure-7@5.[122,1454,225,1444]" captionTargetPageId="5" captionText="Fig. 3. Morphogenesis of Euplotes amieti after protargol impregnation. (A, B) Ventral (A) and dorsal (B) view of the same specimen at an early stage to show the five frontal-ventral-transverse cirral streaks and oral primordium within which membranelles are forming (arrow). (C, D) Ventral (C) and dorsal (D) view of the same specimen at a middle stage to show the completion of the cirral formation, the differen- tiation of caudal cirri at posterior ends of the two rightmost dorsal anlagen (arrowheads), the de novo formation of the new marginal cirri and the frontal cirrus I/1 in both proter and opisthe (arrows). (E, F) Ventral (E) and dorsal (F) view, showing the migration of newly formed cirri and the development of dorsal kineties, arrowheads show the marginal cirri. (G, H) Ventral (G) and dorsal (H) side of the same divider at a late stage showing infraciliature and nuclear apparatus. Scale bars:A–D = 100 μm; E–H = 150 μm." figureDoi="http://doi.org/10.5281/zenodo.12638202" httpUri="https://zenodo.org/record/12638202/files/figure.png" pageId="6" pageNumber="177">Figs 3C</figureCitation>
|
||
,
|
||
<figureCitation id="133561F60A32FFE3FB3686B0FBF7F922" box="[1202,1242,1708,1734]" captionStart="Fig" captionStartId="6.[132,167,1026,1048]" captionTargetBox="[141,1470,225,980]" captionTargetId="figure-7@6.[132,1470,225,980]" captionTargetPageId="6" captionText="Fig. 4. Photomicrographs of Euplotes amieti during morphogenesis after protargol impregnation. (A) Ventral view of a middle divider showing the migration of cirri and the division of the macronucleus. (B, C) Ventral view of an early divider demonstrating two sets of frontal-ventral-transverse cirral streaks (arrowheads) and the oral primordium in opisthe (arrow). (D) To show new cirri derived from the frontal-ventral-transverse cirral anlagen. (E) Ventral view, arrows point to the paroral membrane (PM) in the proter and the development of the PM-anlage in the opisthe; arrowhead indicates the frontal cirrus I/1 in the opisthe formed de novo. (F) Ventral view, indicating the marginal anlagen of the proter (arrow) and the opisthe (arrowhead). (G) Arrowheads showing the dorsal kinety anlage of an early divider. (H) Portion of the ventral view, showing the marginal cirri in the proter (arrow) and the opisthe (arrowhead). (I) Dorsal view, arrowheads indicating the development of the dorsal kinety anlage. (J) Portion of the dorsal view, to show the newly formed caudal cirri in the proter (arrowheads). Scale bars: 100 μm." figureDoi="http://doi.org/10.5281/zenodo.12638204" httpUri="https://zenodo.org/record/12638204/files/figure.png" pageId="6" pageNumber="177">4H</figureCitation>
|
||
).
|
||
</paragraph>
|
||
<paragraph id="8BB17D730A32FFE2FCD286D2FE82FEF8" blockId="6.[816,1463,1465,1942]" lastBlockId="7.[125,772,223,1926]" lastPageId="7" lastPageNumber="178" pageId="6" pageNumber="177">
|
||
Nuclear division: each end of the macronucleus has a recognizable replication band in the early stage (
|
||
<figureCitation id="133561F60A32FFE3FA0386EDFC78F8CA" captionStart="Fig" captionStartId="5.[125,160,1485,1507]" captionTargetBox="[137,1433,240,1427]" captionTargetId="figure-7@5.[122,1454,225,1444]" captionTargetPageId="5" captionText="Fig. 3. Morphogenesis of Euplotes amieti after protargol impregnation. (A, B) Ventral (A) and dorsal (B) view of the same specimen at an early stage to show the five frontal-ventral-transverse cirral streaks and oral primordium within which membranelles are forming (arrow). (C, D) Ventral (C) and dorsal (D) view of the same specimen at a middle stage to show the completion of the cirral formation, the differen- tiation of caudal cirri at posterior ends of the two rightmost dorsal anlagen (arrowheads), the de novo formation of the new marginal cirri and the frontal cirrus I/1 in both proter and opisthe (arrows). (E, F) Ventral (E) and dorsal (F) view, showing the migration of newly formed cirri and the development of dorsal kineties, arrowheads show the marginal cirri. (G, H) Ventral (G) and dorsal (H) side of the same divider at a late stage showing infraciliature and nuclear apparatus. Scale bars:A–D = 100 μm; E–H = 150 μm." figureDoi="http://doi.org/10.5281/zenodo.12638202" httpUri="https://zenodo.org/record/12638202/files/figure.png" pageId="6" pageNumber="177">Fig. 3B</figureCitation>
|
||
). As the replication bands progress, the macronucleus changes shape to become a short strand after the process of replication of the nuclear apparatus (
|
||
<figureCitation id="133561F60A32FFE3FAB08745FA9FF890" box="[1332,1458,1881,1908]" captionStart="Fig" captionStartId="5.[125,160,1485,1507]" captionTargetBox="[137,1433,240,1427]" captionTargetId="figure-7@5.[122,1454,225,1444]" captionTargetPageId="5" captionText="Fig. 3. Morphogenesis of Euplotes amieti after protargol impregnation. (A, B) Ventral (A) and dorsal (B) view of the same specimen at an early stage to show the five frontal-ventral-transverse cirral streaks and oral primordium within which membranelles are forming (arrow). (C, D) Ventral (C) and dorsal (D) view of the same specimen at a middle stage to show the completion of the cirral formation, the differen- tiation of caudal cirri at posterior ends of the two rightmost dorsal anlagen (arrowheads), the de novo formation of the new marginal cirri and the frontal cirrus I/1 in both proter and opisthe (arrows). (E, F) Ventral (E) and dorsal (F) view, showing the migration of newly formed cirri and the development of dorsal kineties, arrowheads show the marginal cirri. (G, H) Ventral (G) and dorsal (H) side of the same divider at a late stage showing infraciliature and nuclear apparatus. Scale bars:A–D = 100 μm; E–H = 150 μm." figureDoi="http://doi.org/10.5281/zenodo.12638202" httpUri="https://zenodo.org/record/12638202/files/figure.png" pageId="6" pageNumber="177">Figs 3D, F</figureCitation>
|
||
,
|
||
<figureCitation id="133561F60A32FFE3FCB48760FC75F872" box="[816,856,1916,1942]" captionStart="Fig" captionStartId="6.[132,167,1026,1048]" captionTargetBox="[141,1470,225,980]" captionTargetId="figure-7@6.[132,1470,225,980]" captionTargetPageId="6" captionText="Fig. 4. Photomicrographs of Euplotes amieti during morphogenesis after protargol impregnation. (A) Ventral view of a middle divider showing the migration of cirri and the division of the macronucleus. (B, C) Ventral view of an early divider demonstrating two sets of frontal-ventral-transverse cirral streaks (arrowheads) and the oral primordium in opisthe (arrow). (D) To show new cirri derived from the frontal-ventral-transverse cirral anlagen. (E) Ventral view, arrows point to the paroral membrane (PM) in the proter and the development of the PM-anlage in the opisthe; arrowhead indicates the frontal cirrus I/1 in the opisthe formed de novo. (F) Ventral view, indicating the marginal anlagen of the proter (arrow) and the opisthe (arrowhead). (G) Arrowheads showing the dorsal kinety anlage of an early divider. (H) Portion of the ventral view, showing the marginal cirri in the proter (arrow) and the opisthe (arrowhead). (I) Dorsal view, arrowheads indicating the development of the dorsal kinety anlage. (J) Portion of the dorsal view, to show the newly formed caudal cirri in the proter (arrowheads). Scale bars: 100 μm." figureDoi="http://doi.org/10.5281/zenodo.12638204" httpUri="https://zenodo.org/record/12638204/files/figure.png" pageId="6" pageNumber="177">4A</figureCitation>
|
||
). As the daughter cells separate, the nodular macronucleus divides to form the 3-shaped nucleus, both in the proter and the opisthe.
|
||
</paragraph>
|
||
<paragraph id="8BB17D730A33FFE2FF278139FD64FDB3" blockId="7.[125,772,223,1926]" pageId="7" pageNumber="178">
|
||
Development of dorsal ciliature: on the dorsal side, the proliferation of new basal bodies occurs in the midline of each parental dorsal kinety in a non-typical primary mode, i.e. dorsal kinety anlagen composed of densely arranged basal bodies are not formed (
|
||
<figureCitation id="133561F60A33FFE2FD1C81ADFDDBFE2F" box="[664,758,433,459]" captionStart="Fig" captionStartId="6.[132,167,1026,1048]" captionTargetBox="[141,1470,225,980]" captionTargetId="figure-7@6.[132,1470,225,980]" captionTargetPageId="6" captionText="Fig. 4. Photomicrographs of Euplotes amieti during morphogenesis after protargol impregnation. (A) Ventral view of a middle divider showing the migration of cirri and the division of the macronucleus. (B, C) Ventral view of an early divider demonstrating two sets of frontal-ventral-transverse cirral streaks (arrowheads) and the oral primordium in opisthe (arrow). (D) To show new cirri derived from the frontal-ventral-transverse cirral anlagen. (E) Ventral view, arrows point to the paroral membrane (PM) in the proter and the development of the PM-anlage in the opisthe; arrowhead indicates the frontal cirrus I/1 in the opisthe formed de novo. (F) Ventral view, indicating the marginal anlagen of the proter (arrow) and the opisthe (arrowhead). (G) Arrowheads showing the dorsal kinety anlage of an early divider. (H) Portion of the ventral view, showing the marginal cirri in the proter (arrow) and the opisthe (arrowhead). (I) Dorsal view, arrowheads indicating the development of the dorsal kinety anlage. (J) Portion of the dorsal view, to show the newly formed caudal cirri in the proter (arrowheads). Scale bars: 100 μm." figureDoi="http://doi.org/10.5281/zenodo.12638204" httpUri="https://zenodo.org/record/12638204/files/figure.png" pageId="7" pageNumber="178">Fig. 4G</figureCitation>
|
||
). With further proliferation, these sparsely distributed anlagen break apart and develop into two groups of kine- tal rows, moving anteriorly and posteriorly, eventually replacing the old structures (
|
||
<figureCitation id="133561F60A33FFE2FE4F8221FD16FDB3" box="[459,571,573,599]" captionStart="Fig" captionStartId="6.[132,167,1026,1048]" captionTargetBox="[141,1470,225,980]" captionTargetId="figure-7@6.[132,1470,225,980]" captionTargetPageId="6" captionText="Fig. 4. Photomicrographs of Euplotes amieti during morphogenesis after protargol impregnation. (A) Ventral view of a middle divider showing the migration of cirri and the division of the macronucleus. (B, C) Ventral view of an early divider demonstrating two sets of frontal-ventral-transverse cirral streaks (arrowheads) and the oral primordium in opisthe (arrow). (D) To show new cirri derived from the frontal-ventral-transverse cirral anlagen. (E) Ventral view, arrows point to the paroral membrane (PM) in the proter and the development of the PM-anlage in the opisthe; arrowhead indicates the frontal cirrus I/1 in the opisthe formed de novo. (F) Ventral view, indicating the marginal anlagen of the proter (arrow) and the opisthe (arrowhead). (G) Arrowheads showing the dorsal kinety anlage of an early divider. (H) Portion of the ventral view, showing the marginal cirri in the proter (arrow) and the opisthe (arrowhead). (I) Dorsal view, arrowheads indicating the development of the dorsal kinety anlage. (J) Portion of the dorsal view, to show the newly formed caudal cirri in the proter (arrowheads). Scale bars: 100 μm." figureDoi="http://doi.org/10.5281/zenodo.12638204" httpUri="https://zenodo.org/record/12638204/files/figure.png" pageId="7" pageNumber="178">Figs 4I, J</figureCitation>
|
||
).
|
||
</paragraph>
|
||
<paragraph id="8BB17D730A33FFE2FF27827CFEC5FCE2" blockId="7.[125,772,223,1926]" pageId="7" pageNumber="178">
|
||
In the proter, two caudal cirri are formed at the posterior end of the right-most two dorsal kinety anlagen (
|
||
<figureCitation id="133561F60A33FFE2FF0182B9FE25FD24" box="[133,264,677,704]" captionStart="Fig" captionStartId="5.[125,160,1485,1507]" captionTargetBox="[137,1433,240,1427]" captionTargetId="figure-7@5.[122,1454,225,1444]" captionTargetPageId="5" captionText="Fig. 3. Morphogenesis of Euplotes amieti after protargol impregnation. (A, B) Ventral (A) and dorsal (B) view of the same specimen at an early stage to show the five frontal-ventral-transverse cirral streaks and oral primordium within which membranelles are forming (arrow). (C, D) Ventral (C) and dorsal (D) view of the same specimen at a middle stage to show the completion of the cirral formation, the differen- tiation of caudal cirri at posterior ends of the two rightmost dorsal anlagen (arrowheads), the de novo formation of the new marginal cirri and the frontal cirrus I/1 in both proter and opisthe (arrows). (E, F) Ventral (E) and dorsal (F) view, showing the migration of newly formed cirri and the development of dorsal kineties, arrowheads show the marginal cirri. (G, H) Ventral (G) and dorsal (H) side of the same divider at a late stage showing infraciliature and nuclear apparatus. Scale bars:A–D = 100 μm; E–H = 150 μm." figureDoi="http://doi.org/10.5281/zenodo.12638202" httpUri="https://zenodo.org/record/12638202/files/figure.png" pageId="7" pageNumber="178">Figs 3C, D</figureCitation>
|
||
,
|
||
<figureCitation id="133561F60A33FFE2FE9082BAFE1FFD24" box="[276,306,678,704]" captionStart="Fig" captionStartId="6.[132,167,1026,1048]" captionTargetBox="[141,1470,225,980]" captionTargetId="figure-7@6.[132,1470,225,980]" captionTargetPageId="6" captionText="Fig. 4. Photomicrographs of Euplotes amieti during morphogenesis after protargol impregnation. (A) Ventral view of a middle divider showing the migration of cirri and the division of the macronucleus. (B, C) Ventral view of an early divider demonstrating two sets of frontal-ventral-transverse cirral streaks (arrowheads) and the oral primordium in opisthe (arrow). (D) To show new cirri derived from the frontal-ventral-transverse cirral anlagen. (E) Ventral view, arrows point to the paroral membrane (PM) in the proter and the development of the PM-anlage in the opisthe; arrowhead indicates the frontal cirrus I/1 in the opisthe formed de novo. (F) Ventral view, indicating the marginal anlagen of the proter (arrow) and the opisthe (arrowhead). (G) Arrowheads showing the dorsal kinety anlage of an early divider. (H) Portion of the ventral view, showing the marginal cirri in the proter (arrow) and the opisthe (arrowhead). (I) Dorsal view, arrowheads indicating the development of the dorsal kinety anlage. (J) Portion of the dorsal view, to show the newly formed caudal cirri in the proter (arrowheads). Scale bars: 100 μm." figureDoi="http://doi.org/10.5281/zenodo.12638204" httpUri="https://zenodo.org/record/12638204/files/figure.png" pageId="7" pageNumber="178">4J</figureCitation>
|
||
). Meanwhile, in the opisthe, two caudal cirri are formed at the end of the right-most two dorsal kinety rows (
|
||
<figureCitation id="133561F60A33FFE2FE9282F7FEF6FCE2" box="[278,475,747,774]" captionStart="Fig" captionStartId="5.[125,160,1485,1507]" captionTargetBox="[137,1433,240,1427]" captionTargetId="figure-7@5.[122,1454,225,1444]" captionTargetPageId="5" captionText="Fig. 3. Morphogenesis of Euplotes amieti after protargol impregnation. (A, B) Ventral (A) and dorsal (B) view of the same specimen at an early stage to show the five frontal-ventral-transverse cirral streaks and oral primordium within which membranelles are forming (arrow). (C, D) Ventral (C) and dorsal (D) view of the same specimen at a middle stage to show the completion of the cirral formation, the differen- tiation of caudal cirri at posterior ends of the two rightmost dorsal anlagen (arrowheads), the de novo formation of the new marginal cirri and the frontal cirrus I/1 in both proter and opisthe (arrows). (E, F) Ventral (E) and dorsal (F) view, showing the migration of newly formed cirri and the development of dorsal kineties, arrowheads show the marginal cirri. (G, H) Ventral (G) and dorsal (H) side of the same divider at a late stage showing infraciliature and nuclear apparatus. Scale bars:A–D = 100 μm; E–H = 150 μm." figureDoi="http://doi.org/10.5281/zenodo.12638202" httpUri="https://zenodo.org/record/12638202/files/figure.png" pageId="7" pageNumber="178">Figs 3C, D, E, F</figureCitation>
|
||
).
|
||
</paragraph>
|
||
<paragraph id="8BB17D730A33FFE2FF278312FE3DFB4D" blockId="7.[125,772,223,1926]" pageId="7" pageNumber="178">
|
||
<emphasis id="B97AA1610A33FFE2FF278312FE0EFCAF" bold="true" pageId="7" pageNumber="178">
|
||
SSU rRNA gene sequence and phylogenetic analyses (
|
||
<figureCitation id="133561F60A33FFE2FF47832DFE3CFCAF" box="[195,273,817,843]" captionStart="Fig" captionStartId="8.[132,167,1518,1540]" captionTargetBox="[159,1427,238,1448]" captionTargetId="figure-7@8.[132,1470,223,1460]" captionTargetPageId="8" captionText="Fig. 5. Phylogenetic tree based on SSU rRNA gene sequences, showing the position of Euplotes amieti (arrow) by Maximum Likelihood (ML) and Bayesian inference (BI). Numbers near branches denote ML bootstrap value/BI posterior probability value. ‘–’ indicates topolo- gies that differ between the ML and BI phylogenies. Fully supported (100%/1.00) branches are marked with solid circles. All branches are drawn to scale. The scale bar corresponds to 5 substitutions per 100 nucleotide positions. GenBank accession numbers are given for each species. Systematic classification is mainly according to Lynn (2008). Euplotid clades I–VI were designated according to Yi et al. (2009)." figureDoi="http://doi.org/10.5281/zenodo.12638206" httpUri="https://zenodo.org/record/12638206/files/figure.png" pageId="7" pageNumber="178">Fig. 5</figureCitation>
|
||
):
|
||
</emphasis>
|
||
The SSU rRNA gene sequence of
|
||
<taxonomicName id="4C0E06F00A33FFE2FD5D832EFE3FFC8A" authorityName="Dragesco" authorityYear="1970" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="7" pageNumber="178" phylum="Ciliophora" rank="species" species="amieti">
|
||
<emphasis id="B97AA1610A33FFE2FD5D832EFE3FFC8A" italics="true" pageId="7" pageNumber="178">Euplotes amieti</emphasis>
|
||
</taxonomicName>
|
||
was deposited in GenBank with accession number KJ524911. The length and GC content of the SSU rRNA gene (not including primers) were 1783 bp and 43.97%, respectively. Phylogenetic trees based on the SSU rRNA gene sequences were constructed using two different methods: Bayesian inference (BI) and maximum-likelihood (ML). The topologies of the BI and ML trees were almost identical and, for purposes of illustration, they were therefore merged into a single tree (
|
||
<figureCitation id="133561F60A33FFE2FF3D8493FE2CFB4D" box="[185,257,1166,1193]" captionStart="Fig" captionStartId="8.[132,167,1518,1540]" captionTargetBox="[159,1427,238,1448]" captionTargetId="figure-7@8.[132,1470,223,1460]" captionTargetPageId="8" captionText="Fig. 5. Phylogenetic tree based on SSU rRNA gene sequences, showing the position of Euplotes amieti (arrow) by Maximum Likelihood (ML) and Bayesian inference (BI). Numbers near branches denote ML bootstrap value/BI posterior probability value. ‘–’ indicates topolo- gies that differ between the ML and BI phylogenies. Fully supported (100%/1.00) branches are marked with solid circles. All branches are drawn to scale. The scale bar corresponds to 5 substitutions per 100 nucleotide positions. GenBank accession numbers are given for each species. Systematic classification is mainly according to Lynn (2008). Euplotid clades I–VI were designated according to Yi et al. (2009)." figureDoi="http://doi.org/10.5281/zenodo.12638206" httpUri="https://zenodo.org/record/12638206/files/figure.png" pageId="7" pageNumber="178">Fig. 5</figureCitation>
|
||
).
|
||
</paragraph>
|
||
<paragraph id="8BB17D730A33FFE2FF2784AEFE48FA79" blockId="7.[125,772,223,1926]" pageId="7" pageNumber="178">
|
||
We included a broad selection of all the SSU rRNA gene sequences of the order
|
||
<taxonomicName id="4C0E06F00A33FFE2FE5284C8FD65FB0A" authorityName="Small & Lynn" authorityYear="1985" box="[470,584,1236,1262]" class="Insecta" higherTaxonomySource="GBIF" kingdom="Animalia" order="Euplotida" pageId="7" pageNumber="178" phylum="Arthropoda" rank="order">Euplotida</taxonomicName>
|
||
from GenBank in the phylogenetic analyses. As shown in
|
||
<figureCitation id="133561F60A33FFE2FD0284EBFDE2FAF6" box="[646,719,1271,1298]" captionStart="Fig" captionStartId="8.[132,167,1518,1540]" captionTargetBox="[159,1427,238,1448]" captionTargetId="figure-7@8.[132,1470,223,1460]" captionTargetPageId="8" captionText="Fig. 5. Phylogenetic tree based on SSU rRNA gene sequences, showing the position of Euplotes amieti (arrow) by Maximum Likelihood (ML) and Bayesian inference (BI). Numbers near branches denote ML bootstrap value/BI posterior probability value. ‘–’ indicates topolo- gies that differ between the ML and BI phylogenies. Fully supported (100%/1.00) branches are marked with solid circles. All branches are drawn to scale. The scale bar corresponds to 5 substitutions per 100 nucleotide positions. GenBank accession numbers are given for each species. Systematic classification is mainly according to Lynn (2008). Euplotid clades I–VI were designated according to Yi et al. (2009)." figureDoi="http://doi.org/10.5281/zenodo.12638206" httpUri="https://zenodo.org/record/12638206/files/figure.png" pageId="7" pageNumber="178">Fig. 5</figureCitation>
|
||
, the order
|
||
<taxonomicName id="4C0E06F00A33FFE2FF418506FE1AFAD0" authorityName="Small & Lynn" authorityYear="1985" box="[197,311,1306,1332]" class="Insecta" higherTaxonomySource="GBIF" kingdom="Animalia" order="Euplotida" pageId="7" pageNumber="178" phylum="Arthropoda" rank="order">Euplotida</taxonomicName>
|
||
is composed of five families, namely
|
||
<taxonomicName id="4C0E06F00A33FFE2FFF98521FE35FAB3" box="[125,280,1341,1367]" class="Hypotrichea" family="Aspidiscidae" kingdom="Chromista" order="Euplotida" pageId="7" pageNumber="178" phylum="Ciliophora" rank="family">Aspidiscidae</taxonomicName>
|
||
,
|
||
<taxonomicName id="4C0E06F00A33FFE2FEA08521FE86FAB3" box="[292,427,1341,1367]" class="Hypotrichea" family="Certesiidae" higherTaxonomySource="GBIF" kingdom="Chromista" order="Euplotida" pageId="7" pageNumber="178" phylum="Ciliophora" rank="family">Certesiidae</taxonomicName>
|
||
,
|
||
<taxonomicName id="4C0E06F00A33FFE2FE338521FD14FAB3" box="[439,569,1341,1367]" class="Hypotrichea" family="Euplotidae" kingdom="Chromista" order="Euplotida" pageId="7" pageNumber="178" phylum="Ciliophora" rank="family">Euplotidae</taxonomicName>
|
||
,
|
||
<taxonomicName id="4C0E06F00A33FFE2FDC18521FDD2FAB3" box="[581,767,1341,1367]" class="Hypotrichea" family="Gastrocirrhidae" kingdom="Chromista" order="Euplotida" pageId="7" pageNumber="178" phylum="Ciliophora" rank="family">Gastrocirrhidae</taxonomicName>
|
||
, and
|
||
<taxonomicName id="4C0E06F00A33FFE2FF37857CFE7AFA9E" box="[179,343,1376,1402]" class="Hypotrichea" family="Uronychiidae" kingdom="Chromista" order="Euplotida" pageId="7" pageNumber="178" phylum="Ciliophora" rank="family">Uronychiidae</taxonomicName>
|
||
. The Order
|
||
<taxonomicName id="4C0E06F00A33FFE2FE6A857CFD4DFA9E" authorityName="Small & Lynn" authorityYear="1985" box="[494,608,1376,1402]" class="Insecta" higherTaxonomySource="GBIF" kingdom="Animalia" order="Euplotida" pageId="7" pageNumber="178" phylum="Arthropoda" rank="order">Euplotida</taxonomicName>
|
||
is monophy- letic in all analyses.
|
||
</paragraph>
|
||
<paragraph id="8BB17D730A33FFE2FF2785BAFD99F862" blockId="7.[125,772,223,1926]" pageId="7" pageNumber="178">
|
||
All available SSU rRNA gene sequences of the
|
||
<taxonomicName id="4C0E06F00A33FFE2FFF985D5FFC9FA07" authorityName="Ehrenberg" authorityYear="1830" box="[125,228,1481,1507]" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="7" pageNumber="178" phylum="Ciliophora" rank="genus">
|
||
<emphasis id="B97AA1610A33FFE2FFF985D5FFC9FA07" box="[125,228,1481,1507]" italics="true" pageId="7" pageNumber="178">Euplotes</emphasis>
|
||
</taxonomicName>
|
||
-complex were included in our phylogenetic analyses. The analyses provide full bootstrap support and posterior probability (PP) for the monophyly of
|
||
<taxonomicName id="4C0E06F00A33FFE2FFF9862EFFD1F9A8" box="[125,252,1586,1612]" class="Hypotrichea" family="Euplotidae" kingdom="Chromista" order="Euplotida" pageId="7" pageNumber="178" phylum="Ciliophora" rank="family">Euplotidae</taxonomicName>
|
||
(100% ML, 1.00 BI). As shown in
|
||
<figureCitation id="133561F60A33FFE2FD30862EFDD0F9A9" box="[692,765,1586,1613]" captionStart="Fig" captionStartId="8.[132,167,1518,1540]" captionTargetBox="[159,1427,238,1448]" captionTargetId="figure-7@8.[132,1470,223,1460]" captionTargetPageId="8" captionText="Fig. 5. Phylogenetic tree based on SSU rRNA gene sequences, showing the position of Euplotes amieti (arrow) by Maximum Likelihood (ML) and Bayesian inference (BI). Numbers near branches denote ML bootstrap value/BI posterior probability value. ‘–’ indicates topolo- gies that differ between the ML and BI phylogenies. Fully supported (100%/1.00) branches are marked with solid circles. All branches are drawn to scale. The scale bar corresponds to 5 substitutions per 100 nucleotide positions. GenBank accession numbers are given for each species. Systematic classification is mainly according to Lynn (2008). Euplotid clades I–VI were designated according to Yi et al. (2009)." figureDoi="http://doi.org/10.5281/zenodo.12638206" httpUri="https://zenodo.org/record/12638206/files/figure.png" pageId="7" pageNumber="178">Fig. 5</figureCitation>
|
||
, all
|
||
<taxonomicName id="4C0E06F00A33FFE2FF2D8649FE3DF98B" authorityName="Ehrenberg" authorityYear="1830" box="[169,272,1621,1647]" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="7" pageNumber="178" phylum="Ciliophora" rank="genus">
|
||
<emphasis id="B97AA1610A33FFE2FF2D8649FE3DF98B" box="[169,272,1621,1647]" italics="true" pageId="7" pageNumber="178">Euplotes</emphasis>
|
||
</taxonomicName>
|
||
-complex species form a well-supported group with full posterior probability and bootstrap values (100% ML, 1.00 BI). This group includes six major clades designated according to
|
||
<bibRefCitation id="EF9F00820A33FFE2FE6286A2FDBBF93C" author="Yi Z. & Song W. & Clamp J. & Chen Z. & Gao S. & Zhang Q." box="[486,662,1726,1752]" pageId="7" pageNumber="178" pagination="599 - 607" refId="ref8751" refString="Yi Z., Song W., Clamp J., Chen Z., Gao S., Zhang Q. (2009) Recon- sideration of systematic relationships within the order Euplotida (Protista, Ciliophora) using new sequences of the gene coding for small-subunit rRNA and testing the use of combined data sets to construct phylogenies of the Diophrys - like species. Mol. Phylogent. Evol. 50: 599 - 607" type="journal article" year="2009">
|
||
Yi
|
||
<emphasis id="B97AA1610A33FFE2FD8C86A2FD16F93C" box="[520,571,1726,1752]" italics="true" pageId="7" pageNumber="178">et al</emphasis>
|
||
. (2009)
|
||
</bibRefCitation>
|
||
based on morphological and phylogenetic information, although support for several of the nodes is rather low (
|
||
<bibRefCitation id="EF9F00820A33FFE2FD02871FFF94F8A5" author="Jiang J. & Zhang Q. & Warren A. & Al-Rasheid K. A. S. & Song W." pageId="7" pageNumber="178" pagination="121 - 132" refId="ref7702" refString="Jiang J., Zhang Q., Warren A., Al-Rasheid K. A. S., Song W. (2010) Morphology and SSU rRNA gene-based phylogeny of two marine Euplotes species, E. orientalis spec. nov. and E. raikovi Agamaliev, 1966 (Ciliophora, Euplotida). Eur. J. Protistol. 46: 121 - 132" type="journal article" year="2010">
|
||
Jiang
|
||
<emphasis id="B97AA1610A33FFE2FD4F8718FDD1F8F9" box="[715,764,1795,1821]" italics="true" pageId="7" pageNumber="178">et al</emphasis>
|
||
. 2010
|
||
</bibRefCitation>
|
||
) and the relationships of
|
||
<taxonomicName id="4C0E06F00A33FFE2FE69873AFC2EF8A4" box="[493,771,1830,1856]" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="7" pageNumber="178" phylum="Ciliophora" rank="species" species="dammamensis">
|
||
<emphasis id="B97AA1610A33FFE2FE69873AFC2EF8A4" box="[493,771,1830,1856]" italics="true" pageId="7" pageNumber="178">Euplotes dammamensis</emphasis>
|
||
</taxonomicName>
|
||
(JX185743),
|
||
<taxonomicName id="4C0E06F00A33FFE2FE9A8755FD1BF887" authorityName="Jiang, Zhang, Hu, Shao, Al-Rasheid & Song" authorityYear="2010" box="[286,566,1865,1891]" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="7" pageNumber="178" phylum="Ciliophora" rank="species" species="parabalteatus">
|
||
<emphasis id="B97AA1610A33FFE2FE9A8755FD1BF887" box="[286,566,1865,1891]" italics="true" pageId="7" pageNumber="178">Euplotes parabalteatus</emphasis>
|
||
</taxonomicName>
|
||
(FJ346568) and
|
||
<taxonomicName id="4C0E06F00A33FFE2FFF98770FE11F862" authorityName="Jiang, Zhang, Hu, Shao, Al-Rasheid & Song" authorityYear="2010" box="[125,316,1900,1926]" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="7" pageNumber="178" phylum="Ciliophora" rank="species" species="sinicus">
|
||
<emphasis id="B97AA1610A33FFE2FFF98770FE11F862" box="[125,316,1900,1926]" italics="true" pageId="7" pageNumber="178">Euplotes sinicus</emphasis>
|
||
</taxonomicName>
|
||
(FJ423448) remain unresolved.
|
||
</paragraph>
|
||
<paragraph id="8BB17D730A33FFE2FCCB80FCFCA4FE2E" blockId="7.[809,1455,223,458]" pageId="7" pageNumber="178">
|
||
We obtained all the SSU rRNA sequences from five isolates of
|
||
<taxonomicName id="4C0E06F00A33FFE2FC28811EFB8EFEF8" authorityName="Wrzesniowski" authorityYear="1870" box="[940,1187,258,284]" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="7" pageNumber="178" phylum="Ciliophora" rank="species" species="eurystomus">
|
||
<emphasis id="B97AA1610A33FFE2FC28811EFB8EFEF8" box="[940,1187,258,284]" italics="true" pageId="7" pageNumber="178">Euplotes eurystomus</emphasis>
|
||
</taxonomicName>
|
||
.
|
||
<taxonomicName id="4C0E06F00A33FFE2FB37811EFA41FEF8" authorityName="Dragesco" authorityYear="1970" box="[1203,1388,258,284]" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="7" pageNumber="178" phylum="Ciliophora" rank="species" species="amieti">
|
||
<emphasis id="B97AA1610A33FFE2FB37811EFA41FEF8" box="[1203,1388,258,284]" italics="true" pageId="7" pageNumber="178">Euplotes amieti</emphasis>
|
||
</taxonomicName>
|
||
clusters with four isolates of
|
||
<taxonomicName id="4C0E06F00A33FFE2FBD4813AFBDAFEDB" authorityName="Wrzesniowski" authorityYear="1870" box="[1104,1271,294,319]" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="7" pageNumber="178" phylum="Ciliophora" rank="species" species="eurystomus">
|
||
<emphasis id="B97AA1610A33FFE2FBD4813AFBDAFEDB" box="[1104,1271,294,319]" italics="true" pageId="7" pageNumber="178">E. eurystomus</emphasis>
|
||
</taxonomicName>
|
||
in Clade I with full support values (100% ML, 1.00 BI), apart from
|
||
<taxonomicName id="4C0E06F00A33FFE2FA018154FCD6FE60" authorityName="Wrzesniowski" authorityYear="1870" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="7" pageNumber="178" phylum="Ciliophora" rank="species" species="eurystomus">
|
||
<emphasis id="B97AA1610A33FFE2FA018154FCD6FE60" italics="true" pageId="7" pageNumber="178">Euplotes eurystomus</emphasis>
|
||
</taxonomicName>
|
||
iso5 (AF452707), which groups with
|
||
<taxonomicName id="4C0E06F00A33FFE2FCAD8192FCFEFE43" authorityName="Pierson" authorityYear="1943" box="[809,979,397,423]" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="7" pageNumber="178" phylum="Ciliophora" rank="species" species="aediculatus">
|
||
<emphasis id="B97AA1610A33FFE2FCAD8192FCFEFE43" box="[809,979,397,423]" italics="true" pageId="7" pageNumber="178">E. aediculatus</emphasis>
|
||
</taxonomicName>
|
||
(EU103618) in Clade I with maximum support.
|
||
</paragraph>
|
||
</subSubSection>
|
||
</treatment>
|
||
</document> |