194 lines
13 KiB
XML
194 lines
13 KiB
XML
<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.33.23670" ID-GBIF-Dataset="42cbc858-8729-4618-9467-590ea7ca068e" ID-PMC="PMC6283267" ID-Pensoft-Pub="1314-4049-33-25" ID-PubMed="30532625" ModsDocAuthor="" ModsDocDate="2018" ModsDocID="1314-4049-33-25" ModsDocOrigin="MycoKeys 33" ModsDocTitle="Identification of endophytic fungi from leaves of Pandanaceae based on their morphotypes and DNA sequence data from southern Thailand" checkinTime="1522305991188" checkinUser="pensoft" docAuthor="Tibpromma, Saowaluck, Hyde, Kevin D., Bhat, Jayarama D., Mortimer, Peter E., Xu, Jianchu, Promputtha, Itthayakorn, Doilom, Mingkwan, Yang, Jun-Bo, Tang, Alvin M. C. & Karunarathna, Samantha C." docDate="2018" docId="6E51AF2B7B605980E6ADA0D1DC7576C2" docLanguage="en" docName="MycoKeys 33: 25-67" docOrigin="MycoKeys 33" docSource="http://dx.doi.org/10.3897/mycokeys.33.23670" docTitle="Mycoleptodiscus endophyticus Tibpromma & K. D. Hyde, sp. nov." docType="treatment" docVersion="6" lastPageNumber="47" masterDocId="B85B6F41CC50A742FFD77F5B3B598C43" masterDocTitle="Identification of endophytic fungi from leaves of Pandanaceae based on their morphotypes and DNA sequence data from southern Thailand" masterLastPageNumber="67" masterPageNumber="25" pageNumber="46" updateTime="1668135980046" updateUser="ExternalLinkService">
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<mods:mods xmlns:mods="http://www.loc.gov/mods/v3">
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<mods:titleInfo>
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<mods:title>Identification of endophytic fungi from leaves of Pandanaceae based on their morphotypes and DNA sequence data from southern Thailand</mods:title>
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</mods:titleInfo>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Tibpromma, Saowaluck</mods:namePart>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Hyde, Kevin D.</mods:namePart>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Bhat, Jayarama D.</mods:namePart>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Mortimer, Peter E.</mods:namePart>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Xu, Jianchu</mods:namePart>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Promputtha, Itthayakorn</mods:namePart>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Doilom, Mingkwan</mods:namePart>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Yang, Jun-Bo</mods:namePart>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Tang, Alvin M. C.</mods:namePart>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Karunarathna, Samantha C.</mods:namePart>
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</mods:name>
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<mods:typeOfResource>text</mods:typeOfResource>
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<mods:relatedItem type="host">
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<mods:titleInfo>
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<mods:title>MycoKeys</mods:title>
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</mods:titleInfo>
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<mods:part>
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<mods:date>2018</mods:date>
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<mods:detail type="volume">
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<mods:number>33</mods:number>
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</mods:detail>
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<mods:extent unit="page">
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<mods:start>25</mods:start>
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<mods:end>67</mods:end>
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</mods:extent>
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</mods:part>
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</mods:relatedItem>
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<mods:location>
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<mods:url>http://dx.doi.org/10.3897/mycokeys.33.23670</mods:url>
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</mods:location>
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<mods:classification>journal article</mods:classification>
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<mods:identifier type="DOI">http://dx.doi.org/10.3897/mycokeys.33.23670</mods:identifier>
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<mods:identifier type="Pensoft-Pub">1314-4049-33-25</mods:identifier>
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</mods:mods>
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<treatment ID-GBIF-Taxon="142664187" LSID="urn:lsid:plazi:treatment:6E51AF2B7B605980E6ADA0D1DC7576C2" httpUri="http://treatment.plazi.org/id/6E51AF2B7B605980E6ADA0D1DC7576C2" lastPageId="22" lastPageNumber="47" pageId="21" pageNumber="46">
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<subSubSection pageId="21" pageNumber="46" type="nomenclature">
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<paragraph pageId="21" pageNumber="46">
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<taxonomicName LSID=" MB823842" authority="Tibpromma & K. D. Hyde" class="Sordariomycetes" family="Magnaporthaceae" genus="Mycoleptodiscus" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Mycoleptodiscus endophyticus" order="Magnaporthales" pageId="21" pageNumber="46" phylum="Ascomycota" rank="species" species="endophyticus">Mycoleptodiscus endophyticus Tibpromma & K.D. Hyde</taxonomicName>
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<taxonomicNameLabel pageId="21" pageNumber="46">sp. nov.</taxonomicNameLabel>
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Figure 17
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</paragraph>
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</subSubSection>
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<subSubSection pageId="21" pageNumber="46" type="etymology">
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<paragraph pageId="21" pageNumber="46">Etymology.</paragraph>
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<paragraph pageId="21" pageNumber="46">Named after its original habitat as an endophytic fungus.</paragraph>
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</subSubSection>
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<subSubSection pageId="21" pageNumber="46" type="holotype">
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<paragraph pageId="21" pageNumber="46">Holotype.</paragraph>
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<paragraph pageId="21" pageNumber="46">MFLU 18-0001</paragraph>
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</subSubSection>
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<subSubSection pageId="21" pageNumber="46" type="description">
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<paragraph pageId="21" pageNumber="46">Culture characteristics.</paragraph>
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<paragraph pageId="21" pageNumber="46">
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<taxonomicName genus="Colonies" lsidName="Colonies" pageId="21" pageNumber="46" rank="genus">Colonies</taxonomicName>
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on PDA (Figure 2, FE101), superficial, dark olivaceous with circular rings with filiform edge and rough and raised on media surface; reverse dark olivaceous.
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<taxonomicName genus="Mycelium" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="Mycelium" pageId="21" pageNumber="46" phylum="Basidiomycota" rank="genus">Mycelium</taxonomicName>
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composed of branched, pale-brown to dark-brown, thick-walled, guttulate, hyphae, with cells sub-globose to ovoid in shape. Not sporulating in culture.
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</paragraph>
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</subSubSection>
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<subSubSection pageId="21" pageNumber="46" type="materials_examined">
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<paragraph pageId="21" pageNumber="46">Material examined.</paragraph>
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<paragraph pageId="21" pageNumber="46">
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THAILAND, Ranong, Muang, on healthy leaves of
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<taxonomicName class="Liliopsida" family="Pandanaceae" genus="Freycinetia" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Freycinetia" order="Pandanales" pageId="21" pageNumber="46" phylum="Tracheophyta" rank="genus">Freycinetia</taxonomicName>
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sp. (
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<taxonomicName family="Pandanaceae" lsidName="Mycelium" pageId="21" pageNumber="46" rank="family">Pandanaceae</taxonomicName>
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), 3 December 2016, S. Tibpromma FE101 (MFLU 18-0001, holotype); HKAS100847, paratype, ex-type living cultures, MFLUCC 17-0545 = KUMCC 17-0263.
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</paragraph>
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</subSubSection>
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<subSubSection pageId="21" pageNumber="46" type="genbank numbers">
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<paragraph pageId="21" pageNumber="46">GenBank numbers.</paragraph>
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<paragraph pageId="21" pageNumber="46">LSU=MG646946, SSU=MG646978, TEF1=MG646985.</paragraph>
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</subSubSection>
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<subSubSection lastPageId="22" lastPageNumber="47" pageId="21" pageNumber="46" type="notes">
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<paragraph pageId="21" pageNumber="46">Notes.</paragraph>
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<paragraph lastPageId="22" lastPageNumber="47" pageId="21" pageNumber="46">
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<taxonomicName class="Sordariomycetes" family="Magnaporthaceae" genus="Mycoleptodiscus" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Mycoleptodiscus" order="Magnaporthales" pageId="21" pageNumber="46" phylum="Ascomycota" rank="genus">Mycoleptodiscus</taxonomicName>
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Ostaz. (1968) belongs to
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<taxonomicName genus="Magnaporthaceae" lsidName="Magnaporthaceae" pageId="21" pageNumber="46" rank="genus">Magnaporthaceae</taxonomicName>
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,
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<taxonomicName genus="Magnaporthales." lsidName="Magnaporthales." pageId="21" pageNumber="46" rank="genus">Magnaporthales.</taxonomicName>
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Since 1968, there have been 17 records of
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<taxonomicName class="Sordariomycetes" family="Magnaporthaceae" genus="Mycoleptodiscus" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Mycoleptodiscus" order="Magnaporthales" pageId="21" pageNumber="46" phylum="Ascomycota" rank="genus">Mycoleptodiscus</taxonomicName>
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in Index Fun
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<pageBreakToken pageId="22" pageNumber="47" start="start">gorum</pageBreakToken>
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. Most of these species were described without molecular data. In this study, a new species
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<taxonomicName class="Sordariomycetes" family="Magnaporthaceae" genus="Mycoleptodiscus" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Mycoleptodiscus endophyticus" order="Magnaporthales" pageId="22" pageNumber="47" phylum="Ascomycota" rank="species" species="endophyticus">Mycoleptodiscus endophyticus</taxonomicName>
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is introduced, based on culture characteristics and phylogenetic analysis (100 % in ML).
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<taxonomicName class="Sordariomycetes" family="Magnaporthaceae" genus="Mycoleptodiscus" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Mycoleptodiscus endophyticus" order="Magnaporthales" pageId="22" pageNumber="47" phylum="Ascomycota" rank="species" species="endophyticus">Mycoleptodiscus endophyticus</taxonomicName>
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was found as an endophytic fungus on leaves of
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<taxonomicName class="Liliopsida" family="Pandanaceae" genus="Freycinetia" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Freycinetia" order="Pandanales" pageId="22" pageNumber="47" phylum="Tracheophyta" rank="genus">Freycinetia</taxonomicName>
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sp;
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<taxonomicName class="Sordariomycetes" family="Magnaporthaceae" genus="Mycoleptodiscus" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Mycoleptodiscus freycinetiae" order="Magnaporthales" pageId="22" pageNumber="47" phylum="Ascomycota" rank="species" species="freycinetiae">Mycoleptodiscus freycinetiae</taxonomicName>
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Whitton, K.D. Hyde & McKenzie was found as a saprobic fungus on the same host but there was no molecular data available to confirm this identification. The authors were unable to compare the morphological differences between the new taxon and
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<taxonomicName class="Sordariomycetes" family="Magnaporthaceae" genus="Mycoleptodiscus" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Mycoleptodiscus freycinetiae" order="Magnaporthales" pageId="22" pageNumber="47" phylum="Ascomycota" rank="species" species="freycinetiae">Mycoleptodiscus freycinetiae</taxonomicName>
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, because only culture characteristics are presented here for this new taxon (Fig. 17).
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</paragraph>
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<caption pageId="22" pageNumber="47">
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<paragraph pageId="22" pageNumber="47">
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Figure 15.
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<taxonomicName class="Sordariomycetes" family="Glomerellaceae" genus="Colletotrichum" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Colletotrichum pandanicola" order="Glomerellales" pageId="22" pageNumber="47" phylum="Ascomycota" rank="species" species="pandanicola">Colletotrichum pandanicola</taxonomicName>
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(MFLU 18-0003, holotype). a Colony on PDA media b
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<taxonomicName class="Arthoniomycetes" family="Arthoniaceae" genus="Conidia" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="Conidia" order="Arthoniales" pageId="22" pageNumber="47" phylum="Ascomycota" rank="genus">Conidia</taxonomicName>
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and conidiogenous cells
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<normalizedToken originalValue="c–g">c-g</normalizedToken>
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<taxonomicName class="Arthoniomycetes" family="Arthoniaceae" genus="Conidia" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="Conidia" order="Arthoniales" pageId="22" pageNumber="47" phylum="Ascomycota" rank="genus">Conidia</taxonomicName>
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on PDA culture. Scale bars: 5
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<normalizedToken originalValue="μm">μm</normalizedToken>
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(b), 2
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<normalizedToken originalValue="μm">μm</normalizedToken>
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(
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<normalizedToken originalValue="c–g">c-g</normalizedToken>
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).
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</paragraph>
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</caption>
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<caption pageId="22" pageNumber="47">
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<paragraph pageId="22" pageNumber="47">
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Figure 16. Phylogram generated from maximum likelihood analysis based on combined ITS, LSU, SSU and TEF1 sequenced data. Maximum parsimony bootstrap values are given above/below the nodes. The newly generated sequences are in red bold. The tree is rooted with
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<taxonomicName class="Sordariomycetes" family="Thyridiaceae" genus="Thyridium" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Thyridium vestitum" order="Amphisphaeriales" pageId="22" pageNumber="47" phylum="Ascomycota" rank="species" species="vestitum">Thyridium vestitum</taxonomicName>
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.
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</paragraph>
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</caption>
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<caption pageId="22" pageNumber="47">
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<paragraph pageId="22" pageNumber="47">
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Figure 17.
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<taxonomicName class="Sordariomycetes" family="Magnaporthaceae" genus="Mycoleptodiscus" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Mycoleptodiscus endophyticus" order="Magnaporthales" pageId="22" pageNumber="47" phylum="Ascomycota" rank="species" species="endophyticus">Mycoleptodiscus endophyticus</taxonomicName>
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(MFLU 18-0001, holotype). a Colony on MEA media b, c Mycelia masses
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<normalizedToken originalValue="d–f">d-f</normalizedToken>
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Vegetative hyphae in culture. Scale bars: 10
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<normalizedToken originalValue="μm">μm</normalizedToken>
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(
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<normalizedToken originalValue="b–d">b-d</normalizedToken>
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), 5
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<normalizedToken originalValue="μm">μm</normalizedToken>
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(e, f).
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</paragraph>
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</caption>
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</subSubSection>
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</treatment>
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</document> |