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<document id="1608807D932EF9840AB70D0787FA3CEF" ID-DOI="10.4467/16890027AP.14.015.1596" ID-ISSN="1689-0027" ID-Zenodo-Dep="10371131" IM.bibliography_approvedBy="felipe" IM.illustrations_approvedBy="felipe" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.tables_requiresApprovalFor="GgImagineBatch" IM.taxonomicNames_approvedBy="felipe" IM.treatments_approvedBy="felipe" checkinTime="1702473367385" checkinUser="tatiana" docAuthor="Foissner, Wilhelm, Wolf, Klaus W., Kumar, Santosh &amp; Quintela-Alonso, Kuidong Xu and Pablo" docDate="2014" docId="7F52878BFFDEFF83FF548ACBFD2200C0" docLanguage="en" docName="ActaProtozool.53.2.159-194.pdf" docOrigin="Acta Protozoologica 53" docStyle="DocumentStyle:64ABA4BEA387EE5C24A0DDEB32CC92E7.2:ActaProtozool.2014-.journal_article" docStyleId="64ABA4BEA387EE5C24A0DDEB32CC92E7" docStyleName="ActaProtozool.2014-.journal_article" docStyleVersion="2" docTitle="Spathidium wolfi Foissner &amp; Wolf &amp; Kumar &amp; Quintela-Alonso 2014, nov. spec." docType="treatment" docVersion="2" lastPageNumber="193" masterDocId="836BFFF3FFC2FFA1FFD08C52FF97034C" masterDocTitle="Five New Spathidiids (Ciliophora: Bromeliads Haptoria) from Caribbean Tank" masterLastPageNumber="194" masterPageNumber="159" pageNumber="187" updateTime="1702473958120" updateUser="ExternalLinkService" zenodo-license-document="CC-BY-4.0">
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<mods:title id="6E80241A19D7351C6BEEE534AE852810">Five New Spathidiids (Ciliophora: Bromeliads Haptoria) from Caribbean Tank</mods:title>
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<mods:namePart id="B6C6AC0FDDAB4CC3B98543B9D9E52E54">Foissner, Wilhelm</mods:namePart>
<mods:affiliation id="F9B1982B1B73AE538A2396FAE5DA5F85">Universität Salzburg, FB Organismische Biologie, Salzburg, Austria;</mods:affiliation>
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<mods:namePart id="C6AC3A9D28ED52777AAE9729B6049106">Wolf, Klaus W.</mods:namePart>
<mods:affiliation id="58AA2505CE4BE06E79BA80266B15A72A">University of the West Indies, Electron Microscopy Unit, Kingston, Jamaica;</mods:affiliation>
<mods:nameIdentifier id="AB31FAD5BE2A373DA62D32E6824F3518" type="email">wilhelm.foissner@sbg.ac.at</mods:nameIdentifier>
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<mods:namePart id="ABCD45084304547F0F52BD27DB389D8B">Kumar, Santosh</mods:namePart>
<mods:affiliation id="A1C4D44E5B3DCD8B53C634033B2FF3A7">Universität Salzburg, FB Organismische Biologie, Salzburg, Austria;</mods:affiliation>
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<mods:namePart id="1F2B84B616BCA7E5827F1AA7A26BB181">Quintela-Alonso, Kuidong Xu and Pablo</mods:namePart>
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<mods:title id="33669A570E988BB5D07A0A3EC0057945">Acta Protozoologica</mods:title>
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<mods:date id="8DB541986EAF7DBD3EC8D61F154A5582">2014</mods:date>
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<mods:number id="FD498DFD0B3B365B625E3CCC932074C0">53</mods:number>
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<treatment id="7F52878BFFDEFF83FF548ACBFD2200C0" LSID="urn:lsid:plazi:treatment:7F52878BFFDEFF83FF548ACBFD2200C0" httpUri="http://treatment.plazi.org/id/7F52878BFFDEFF83FF548ACBFD2200C0" lastPageId="34" lastPageNumber="193" pageId="28" pageNumber="187">
<subSubSection id="BFE16516FFDEFFBDFF548ACBFC9D05FF" box="[132,778,1689,1716]" pageId="28" pageNumber="187" type="nomenclature">
<paragraph id="F744369DFFDEFFBDFF548ACBFC9D05FF" blockId="28.[131,778,1689,1751]" box="[132,778,1689,1716]" pageId="28" pageNumber="187">
<heading id="AC0C81F1FFDEFFBDFF548ACBFC9D05FF" bold="true" box="[132,778,1689,1716]" centered="true" fontSize="11" level="2" pageId="28" pageNumber="187" reason="4">
<emphasis id="C58FEA8FFFDEFFBDFF548ACBFC9D05FF" bold="true" box="[132,778,1689,1716]" pageId="28" pageNumber="187">
<taxonomicName id="30FB4D1EFFDEFFBDFF548ACBFEC505F8" authority="Foissner &amp; Wolf &amp; Kumar &amp; Quintela-Alonso, 2014" authorityName="Foissner &amp; Wolf &amp; Kumar &amp; Quintela-Alonso" authorityYear="2014" box="[132,338,1689,1716]" class="Gymnostomatea" family="Spathidiidae" genus="Spathidium" kingdom="Chromista" order="Spathidiida" pageId="28" pageNumber="187" phylum="Ciliophora" rank="species" species="wolfi" status="sp. nov.">
<emphasis id="C58FEA8FFFDEFFBDFF548ACBFEC505F8" bold="true" box="[132,338,1689,1716]" italics="true" pageId="28" pageNumber="187">Spathidium wolfi</emphasis>
</taxonomicName>
<taxonomicNameLabel id="DEBC57F4FFDEFFBDFE888AC8FE5905F8" box="[344,462,1690,1716]" pageId="28" pageNumber="187" rank="species">nov. spec.</taxonomicNameLabel>
(
<figureCitation id="6FC02A18FFDEFFBDFE0C8ACBFDCA05FF" box="[476,605,1689,1715]" captionStart="Figs 19" captionStartId="30.[132,175,1353,1375]" captionTargetBox="[135,1457,228,1289]" captionTargetId="figure-7@30.[132,1457,223,1309]" captionTargetPageId="30" captionText="Figs 19al. Spathidium wolfi from life (ah) and after protargol impregnation (il). a right side view of a representative specimen, length 140 µm. The arrowhead marks the anterior contractile vacuole; b frontal view of oral bulge; c oral bulge extrusomes, length 10 µm; d developing extrusomes in the cytoplasm; e slender shape variant, showing the two contractile vacuoles (arrowheads); f development of the “Ringgranula” (cp. Fig. 19a); g, h surface view and optical section, showing the cortical granulation; i, j ventral and dorsal view of anterior body region of a paratype specimen, showing the isostichad dorsal brush and the cuneate oral bulge; k, l right and left side view of anterior body region of the holotype specimen (cp. Figs 20a, b). B(13) dorsal brush rows, BA oral basket, BU oral bulge, CK circumoral kinety, E extrusomes, F oral bulge fibres, G cortical granules, L lipid droplet, MA macronucleus, RG “Ringgranula”. Scale bars: 15 µm (il) and 50 µm (a)." figureDoi="http://doi.org/10.5281/zenodo.10371178" httpUri="https://zenodo.org/record/10371178/files/figure.png" pageId="28" pageNumber="187">Figs 19al</figureCitation>
,
<figureCitation id="6FC02A18FFDEFFBDFDB98ACBFD3905FF" box="[617,686,1689,1715]" captionStart="Figs 20" captionStartId="31.[125,168,1640,1662]" captionTargetBox="[146,1430,226,1570]" captionTargetId="figure-8@31.[125,1450,223,1587]" captionTargetPageId="31" captionText="Figs 20af. Spathidium wolfi from life (c) and after protargol impregnation (a, b, df). a, b ciliary pattern of right and left side and nuclear apparatus of holotype specimen; for oral details, see Figs 19k, l. The arrowhead marks the excretory pores of the anterior contractile vacu- ole, i.e., the main character of this species; c supposed structure of dorsal brush row 3; d ventral view of a paratype specimen with open circumoral kinety (arrow) and thus resembling Apertospathula; e dorsolateral view of a paratype specimen, showing the ciliary pattern and the excretory pores of the anterior and posterior contractile vacuole; f ventrolateral view of oral body region, showing the Spathidium ciliary pattern. B(13) dorsal brush (rows), BU oral bulge, CK circumoral kinety, CV contractile vacuole, EP excretory pores, MA macronucleus, MI micronuclei. Scale bars: 10 µm (d, f) and 50 µm (a, b, e)." figureDoi="http://doi.org/10.5281/zenodo.10371180" httpUri="https://zenodo.org/record/10371180/files/figure.png" pageId="28" pageNumber="187">20af</figureCitation>
,
<figureCitation id="6FC02A18FFDEFFBDFD6B8ACBFC9305FF" box="[699,772,1689,1715]" captionStart="Figs 21" captionStartId="32.[132,175,1013,1035]" captionTargetBox="[132,1457,223,981]" captionTargetId="figure-7@32.[132,1457,223,990]" captionTargetPageId="32" captionText="Figs 21ac. Spathidium wolfi, dorsal views of anterior body region after protargol impregnation, showing the isostichad dorsal brush (c, arrows) and the excretory pores (arrowheads) of the anterior contractile vacuole. B(13) dorsal brush rows, BU oral bulge, CK cir- cumoral kinety, MA macronucleus. Scale bars: 20 µm." figureDoi="http://doi.org/10.5281/zenodo.10371184" httpUri="https://zenodo.org/record/10371184/files/figure.png" pageId="28" pageNumber="187">21ac</figureCitation>
,
</emphasis>
</heading>
</paragraph>
</subSubSection>
<subSubSection id="BFE16516FFDEFFBDFF538AEFFED3059B" box="[131,324,1725,1751]" pageId="28" pageNumber="187" type="description">
<paragraph id="F744369DFFDEFFBDFF538AEFFED3059B" blockId="28.[131,778,1689,1751]" box="[131,324,1725,1751]" pageId="28" pageNumber="187">
<emphasis id="C58FEA8FFFDEFFBDFF538AEFFED3059B" bold="true" box="[131,324,1725,1751]" pageId="28" pageNumber="187">
<figureCitation id="6FC02A18FFDEFFBDFF538AEFFF45059B" box="[131,210,1725,1751]" captionStart="Figs 22" captionStartId="33.[125,168,1750,1772]" captionTargetBox="[125,1450,228,1713]" captionTargetId="figure-8@33.[125,1450,223,1725]" captionTargetPageId="33" captionText="Figs 22ak. Spathidium wolfi after protargol impregnation. a a slender specimen; b overview of a specimen inflated by the prepara- tion; c, d overviews showing the moniliform macronucleus; eg ventral views, showing the obovate (or broadly cuneate) circumoral kinety; h ventrolateral view, showing kineties and cortical granules; ik anterior body portion at three focal planes. (i) Right side surface view, showing fibres in oral bulge and somatic cortex (arrowheads). (j) When focused slightly deeper, the nematodesmata become visible. (k) When focused to the left side, the dorsal brush and the Spathidium ciliary pattern become recognizable. B(13) dorsal brush (rows), BU oral bulge, CK circumoral kinety, E extrusomes, EP excretory pores, F fibres, G cortical granules, K kineties, MA mac- ronucleus, MI micronuclei, N nematodesmata. Scale bars: 10 µm (ek) and 50 µm (ad)." figureDoi="http://doi.org/10.5281/zenodo.10371186" httpUri="https://zenodo.org/record/10371186/files/figure.png" pageId="28" pageNumber="187">22ak</figureCitation>
;
<tableCitation id="BA790326FFDEFFBDFF0F8AEFFEAC059B" box="[223,315,1725,1751]" captionStart="Table 7" captionStartId="29.[125,181,223,244]" captionText="Table 7. Morphometric data on Spathidium wolfi." pageId="28" pageNumber="187">Table 7</tableCitation>
)
</emphasis>
</paragraph>
</subSubSection>
<subSubSection id="BFE16516FFDEFFBDFF7A8AA2FB5505A5" pageId="28" pageNumber="187" type="diagnosis">
<paragraph id="F744369DFFDEFFBDFF7A8AA2FB5505A5" blockId="28.[132,779,1776,1945]" lastBlockId="28.[815,1463,1603,1945]" pageId="28" pageNumber="187">
<emphasis id="C58FEA8FFFDEFFBDFF7A8AA2FEBB0446" bold="true" box="[170,300,1776,1802]" pageId="28" pageNumber="187">Diagnosis:</emphasis>
Size about 135 × 25 µm
<emphasis id="C58FEA8FFFDEFFBDFDBE8AA3FD560446" box="[622,705,1777,1802]" italics="true" pageId="28" pageNumber="187">in vivo</emphasis>
. Very narrowly spatulate to bluntly fusiform with oblique, cuneate oral bulge half as long as widest trunk region. Macronucleus moniliform, composed of an average of 8 ellipsoidal nodules; multimicronucleate. Two contractile vacuoles, one dorsally at margin of first and second body third, the other in rear end. On average 15 ciliary rows, 3 of them differentiated to isostichad, short dorsal brush occupying 19% of body length; 14 widely spaced dikinetids in row 3.
</paragraph>
</subSubSection>
<subSubSection id="BFE16516FFDEFFBCFC858AA1FF5C0449" lastPageId="29" lastPageNumber="188" pageId="28" pageNumber="187" type="materials_examined">
<paragraph id="F744369DFFDEFFBDFC858AA1FC7D041F" blockId="28.[815,1463,1603,1945]" pageId="28" pageNumber="187">
<materialsCitation id="47933CC0FFDEFFBDFC858AA1FC71041F" country="Jamaica" county="Spanish Town" latitude="18.0" location="Tanks" longLatPrecision="39787" longitude="-77.0" municipality="Mt. Diablo" pageId="28" pageNumber="187" specimenCount="1" typeStatus="holotype">
<emphasis id="C58FEA8FFFDEFFBDFC858AA1FC680440" bold="true" box="[853,1023,1778,1805]" pageId="28" pageNumber="187">
<typeStatus id="2840883FFFDEFFBDFC858AA1FC050441" box="[853,914,1779,1805]" pageId="28" pageNumber="187">Type</typeStatus>
locality:
</emphasis>
<location id="F2246046FFDEFFBDFBD68AA0FBDB0440" LSID="urn:lsid:plazi:treatment:7F52878BFFDEFF83FF548ACBFD2200C0:F2246046FFDEFFBDFBD68AA0FBDB0440" box="[1030,1100,1778,1804]" country="Jamaica" county="Spanish Town" latitude="18.0" longLatPrecision="39787" longitude="-77.0" municipality="Mt. Diablo" name="Tanks" pageId="28" pageNumber="187">Tanks</location>
of terrestrial bromeliads at the foot of
<collectingMunicipality id="1720ACE7FFDEFFBDFC568B44FB9C047C" box="[902,1035,1814,1840]" pageId="28" pageNumber="187">Mt. Diablo</collectingMunicipality>
, northwest of
<collectingCounty id="1E254E11FFDEFFBDFB628B44FACC047C" box="[1202,1371,1814,1840]" pageId="28" pageNumber="187">Spanish Town</collectingCounty>
,
<collectingCountry id="8FEC760DFFDEFFBDFAB78B44FCDB041F" name="Jamaica" pageId="28" pageNumber="187">Jamaica</collectingCountry>
,
<geoCoordinate id="92CF505AFFDEFFBDFC888B6BFC00041F" box="[856,919,1849,1875]" degrees="18" direction="north" orientation="latitude" pageId="28" pageNumber="187" precision="55555" value="18.0">N18°</geoCoordinate>
<geoCoordinate id="92CF505AFFDEFFBDFC4D8B6BFC71041F" box="[925,998,1849,1875]" degrees="77" direction="west" orientation="longitude" pageId="28" pageNumber="187" precision="55555" value="-77.0">W77°</geoCoordinate>
</materialsCitation>
.
</paragraph>
<paragraph id="F744369DFFDEFFBCFC858B0EFE8C051B" blockId="28.[815,1463,1603,1945]" lastBlockId="29.[125,773,1493,1936]" lastPageId="29" lastPageNumber="188" pageId="28" pageNumber="187">
<emphasis id="C58FEA8FFFDEFFBDFC858B0EFB84043A" bold="true" box="[853,1043,1884,1910]" pageId="28" pageNumber="187">Type material:</emphasis>
<materialsCitation id="47933CC0FFDEFFBCFBF08B0EFE80051B" country="Austria" county="Relevant" lastPageId="29" lastPageNumber="188" location="Biologiezentrum" municipality="Oberosterreichische Landesmuseum in Linz" pageId="28" pageNumber="187" specimenCount="4" typeStatus="holotype">
<specimenCount id="E1FDFD14FFDEFFBDFBF08B0EFB35043A" box="[1056,1186,1884,1910]" count="1" pageId="28" pageNumber="187" type="generic" typeStatus="holotype">1 holotype</specimenCount>
and
<specimenCount id="E1FDFD14FFDEFFBDFB358B0EFAF1043A" box="[1253,1382,1884,1910]" count="3" pageId="28" pageNumber="187" type="generic" typeStatus="paratype">3 paratype</specimenCount>
slides with protargol-impregnated specimens have been deposited in the
<location id="F2246046FFDFFFBCFEE98987FE6906A3" LSID="urn:lsid:plazi:treatment:7F52878BFFDEFF83FF548ACBFD2200C0:F2246046FFDFFFBCFEE98987FE6906A3" box="[313,510,1493,1519]" country="Austria" county="Relevant" municipality="Oberosterreichische Landesmuseum in Linz" name="Biologiezentrum" pageId="29" pageNumber="188">Biologiezentrum</location>
of the
<collectingMunicipality id="1720ACE7FFDFFFBCFDB78987FE66055E" pageId="29" pageNumber="188">Oberösterreichische Landesmuseum in Linz</collectingMunicipality>
(LI),
<collectingCountry id="8FEC760DFFDFFFBCFDE589AAFD18055E" box="[565,655,1528,1554]" name="Austria" pageId="29" pageNumber="188">Austria</collectingCountry>
.
<collectingCounty id="1E254E11FFDFFFBCFD4B89AAFC94055E" box="[667,771,1528,1554]" pageId="29" pageNumber="188">Relevant</collectingCounty>
specimens have been marked by black ink circles on the coverslip
</materialsCitation>
.
</paragraph>
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<paragraph id="F744369DFFDFFFBCFFAD8C8DFDCF03B9" blockId="29.[125,600,223,245]" box="[125,600,223,245]" pageId="29" pageNumber="188">
<emphasis id="C58FEA8FFFDFFFBCFFAD8C8DFF5A03B8" bold="true" box="[125,205,223,244]" pageId="29" pageNumber="188">Table 7.</emphasis>
Morphometric data on
<taxonomicName id="30FB4D1EFFDFFFBCFE608C8DFDC503B8" authorityName="Foissner &amp; Wolf &amp; Kumar &amp; Quintela-Alonso" authorityYear="2014" box="[432,594,223,244]" class="Gymnostomatea" family="Spathidiidae" genus="Spathidium" kingdom="Chromista" order="Spathidiida" pageId="29" pageNumber="188" phylum="Ciliophora" rank="species" species="wolfi">
<emphasis id="C58FEA8FFFDFFFBCFE608C8DFDC503B8" box="[432,594,223,244]" italics="true" pageId="29" pageNumber="188">Spathidium wolfi</emphasis>
</taxonomicName>
.
</paragraph>
</caption>
<paragraph id="F744369DFFDFFFBCFFAD8D44FA1D07B5" pageId="29" pageNumber="188">
<table id="85FBC43DFFDF005EFFAD8D46FA1D07B5" box="[125,1418,276,1273]" colsContinueFrom="24.[132,1430,962,1847]" gridcols="9" gridrows="27" pageId="29" pageNumber="188">
<tr id="49CB34DFFFDF005EFFAD8D46FA1D0267" box="[125,1418,276,299]" gridrow="0" pageId="29" pageNumber="188">
<th id="0A1A5DA3FFDF005EFFAD8D46FDF10267" box="[125,614,276,299]" gridcol="0" gridrow="0" pageId="29" pageNumber="188">Characteristicsa</th>
<th id="0A1A5DA3FFDF005EFD6F8D46FD790267" box="[703,750,276,299]" gridcol="1" gridrow="0" pageId="29" pageNumber="188">
<emphasis id="C58FEA8FFFDFFFBCFD1D8D4AFD400267" box="[717,727,280,299]" italics="true" pageId="29" pageNumber="188"></emphasis>
</th>
<th id="0A1A5DA3FFDF005EFCF18D46FCC70267" box="[801,848,276,299]" gridcol="2" gridrow="0" pageId="29" pageNumber="188">M</th>
<th id="0A1A5DA3FFDF005EFC548D46FC3D0267" box="[900,938,276,299]" gridcol="3" gridrow="0" pageId="29" pageNumber="188">SD</th>
<th id="0A1A5DA3FFDF005EFC3B8D46FB9B0267" box="[1003,1036,276,299]" gridcol="4" gridrow="0" pageId="29" pageNumber="188">SE</th>
<th id="0A1A5DA3FFDF005EFB988D46FBF90267" box="[1096,1134,276,299]" gridcol="5" gridrow="0" pageId="29" pageNumber="188">CV</th>
<th id="0A1A5DA3FFDF005EFB778D46FB4F0267" box="[1191,1240,276,299]" gridcol="6" gridrow="0" pageId="29" pageNumber="188">Min</th>
<th id="0A1A5DA3FFDF005EFAD98D46FAAE0267" box="[1289,1337,276,299]" gridcol="7" gridrow="0" pageId="29" pageNumber="188">Max</th>
<th id="0A1A5DA3FFDF005EFAA58D46FA1D0267" box="[1397,1418,276,299]" gridcol="8" gridrow="0" pageId="29" pageNumber="188">n</th>
</tr>
<tr id="49CB34DFFFDF005EFFAD8D6FFA1D021C" box="[125,1418,317,336]" gridrow="1" pageId="29" pageNumber="188">
<th id="0A1A5DA3FFDF005EFFAD8D6FFDF1021C" box="[125,614,317,336]" gridcol="0" gridrow="1" pageId="29" pageNumber="188">Body, length, µm</th>
<td id="0A1A5DA3FFDF005EFD6F8D6FFD79021C" box="[703,750,317,336]" gridcol="1" gridrow="1" pageId="29" pageNumber="188">118.9</td>
<td id="0A1A5DA3FFDF005EFCF18D6FFCC7021C" box="[801,848,317,336]" gridcol="2" gridrow="1" pageId="29" pageNumber="188">117.0</td>
<td id="0A1A5DA3FFDF005EFC548D6FFC3D021C" box="[900,938,317,336]" gridcol="3" gridrow="1" pageId="29" pageNumber="188">10.8</td>
<td id="0A1A5DA3FFDF005EFC3B8D6FFB9B021C" box="[1003,1036,317,336]" gridcol="4" gridrow="1" pageId="29" pageNumber="188">2.4</td>
<td id="0A1A5DA3FFDF005EFB988D6FFBF9021C" box="[1096,1134,317,336]" gridcol="5" gridrow="1" pageId="29" pageNumber="188">9.1</td>
<td id="0A1A5DA3FFDF005EFB778D6FFB4F021C" box="[1191,1240,317,336]" gridcol="6" gridrow="1" pageId="29" pageNumber="188">100.0</td>
<td id="0A1A5DA3FFDF005EFAD98D6FFAAE021C" box="[1289,1337,317,336]" gridcol="7" gridrow="1" pageId="29" pageNumber="188">145.0</td>
<td id="0A1A5DA3FFDF005EFAA58D6FFA1D021C" box="[1397,1418,317,336]" gridcol="8" gridrow="1" pageId="29" pageNumber="188">21</td>
</tr>
<tr id="49CB34DFFFDF005EFFAD8D30FA1D023A" box="[125,1418,354,374]" gridrow="2" pageId="29" pageNumber="188">
<th id="0A1A5DA3FFDF005EFFAD8D30FDF1023A" box="[125,614,354,374]" gridcol="0" gridrow="2" pageId="29" pageNumber="188">Body, width, µm</th>
<td id="0A1A5DA3FFDF005EFD6F8D30FD79023A" box="[703,750,354,374]" gridcol="1" gridrow="2" pageId="29" pageNumber="188">29.9</td>
<td id="0A1A5DA3FFDF005EFCF18D30FCC7023A" box="[801,848,354,374]" gridcol="2" gridrow="2" pageId="29" pageNumber="188">30.0</td>
<td id="0A1A5DA3FFDF005EFC548D30FC3D023A" box="[900,938,354,374]" gridcol="3" gridrow="2" pageId="29" pageNumber="188">4.9</td>
<td id="0A1A5DA3FFDF005EFC3B8D30FB9B023A" box="[1003,1036,354,374]" gridcol="4" gridrow="2" pageId="29" pageNumber="188">1.1</td>
<td id="0A1A5DA3FFDF005EFB988D30FBF9023A" box="[1096,1134,354,374]" gridcol="5" gridrow="2" pageId="29" pageNumber="188">16.4</td>
<td id="0A1A5DA3FFDF005EFB778D30FB4F023A" box="[1191,1240,354,374]" gridcol="6" gridrow="2" pageId="29" pageNumber="188">21.0</td>
<td id="0A1A5DA3FFDF005EFAD98D30FAAE023A" box="[1289,1337,354,374]" gridcol="7" gridrow="2" pageId="29" pageNumber="188">44.0</td>
<td id="0A1A5DA3FFDF005EFAA58D30FA1D023A" box="[1397,1418,354,374]" gridcol="8" gridrow="2" pageId="29" pageNumber="188">21</td>
</tr>
<tr id="49CB34DFFFDF005EFFAD8DDAFA1D02D7" box="[125,1418,392,411]" gridrow="3" pageId="29" pageNumber="188">
<th id="0A1A5DA3FFDF005EFFAD8DDAFDF102D7" box="[125,614,392,411]" gridcol="0" gridrow="3" pageId="29" pageNumber="188">Body length:width, ratio</th>
<td id="0A1A5DA3FFDF005EFD6F8DDAFD7902D7" box="[703,750,392,411]" gridcol="1" gridrow="3" pageId="29" pageNumber="188">4.1</td>
<td id="0A1A5DA3FFDF005EFCF18DDAFCC702D7" box="[801,848,392,411]" gridcol="2" gridrow="3" pageId="29" pageNumber="188">3.9</td>
<td id="0A1A5DA3FFDF005EFC548DDAFC3D02D7" box="[900,938,392,411]" gridcol="3" gridrow="3" pageId="29" pageNumber="188">0.9</td>
<td id="0A1A5DA3FFDF005EFC3B8DDAFB9B02D7" box="[1003,1036,392,411]" gridcol="4" gridrow="3" pageId="29" pageNumber="188">0.2</td>
<td id="0A1A5DA3FFDF005EFB988DDAFBF902D7" box="[1096,1134,392,411]" gridcol="5" gridrow="3" pageId="29" pageNumber="188">22.3</td>
<td id="0A1A5DA3FFDF005EFB778DDAFB4F02D7" box="[1191,1240,392,411]" gridcol="6" gridrow="3" pageId="29" pageNumber="188">2.5</td>
<td id="0A1A5DA3FFDF005EFAD98DDAFAAE02D7" box="[1289,1337,392,411]" gridcol="7" gridrow="3" pageId="29" pageNumber="188">6.0</td>
<td id="0A1A5DA3FFDF005EFAA58DDAFA1D02D7" box="[1397,1418,392,411]" gridcol="8" gridrow="3" pageId="29" pageNumber="188">21</td>
</tr>
<tr id="49CB34DFFFDF005EFFAD8DFFFA1D028D" box="[125,1418,429,449]" gridrow="4" pageId="29" pageNumber="188">
<th id="0A1A5DA3FFDF005EFFAD8DFFFDF1028D" box="[125,614,429,449]" gridcol="0" gridrow="4" pageId="29" pageNumber="188">Oral bulge, length, µm</th>
<td id="0A1A5DA3FFDF005EFD6F8DFFFD79028D" box="[703,750,429,449]" gridcol="1" gridrow="4" pageId="29" pageNumber="188">14.6</td>
<td id="0A1A5DA3FFDF005EFCF18DFFFCC7028D" box="[801,848,429,449]" gridcol="2" gridrow="4" pageId="29" pageNumber="188">14.0</td>
<td id="0A1A5DA3FFDF005EFC548DFFFC3D028D" box="[900,938,429,449]" gridcol="3" gridrow="4" pageId="29" pageNumber="188">1.4</td>
<td id="0A1A5DA3FFDF005EFC3B8DFFFB9B028D" box="[1003,1036,429,449]" gridcol="4" gridrow="4" pageId="29" pageNumber="188">0.3</td>
<td id="0A1A5DA3FFDF005EFB988DFFFBF9028D" box="[1096,1134,429,449]" gridcol="5" gridrow="4" pageId="29" pageNumber="188">9.8</td>
<td id="0A1A5DA3FFDF005EFB778DFFFB4F028D" box="[1191,1240,429,449]" gridcol="6" gridrow="4" pageId="29" pageNumber="188">12.0</td>
<td id="0A1A5DA3FFDF005EFAD98DFFFAAE028D" box="[1289,1337,429,449]" gridcol="7" gridrow="4" pageId="29" pageNumber="188">17.0</td>
<td id="0A1A5DA3FFDF005EFAA58DFFFA1D028D" box="[1397,1418,429,449]" gridcol="8" gridrow="4" pageId="29" pageNumber="188">21</td>
</tr>
<tr id="49CB34DFFFDF005EFFAD8D81FA1D02AA" box="[125,1418,467,486]" gridrow="5" pageId="29" pageNumber="188">
<th id="0A1A5DA3FFDF005EFFAD8D81FDF102AA" box="[125,614,467,486]" gridcol="0" gridrow="5" pageId="29" pageNumber="188">Body width:oral bulge length, ratio</th>
<td id="0A1A5DA3FFDF005EFD6F8D81FD7902AA" box="[703,750,467,486]" gridcol="1" gridrow="5" pageId="29" pageNumber="188">2.1</td>
<td id="0A1A5DA3FFDF005EFCF18D81FCC702AA" box="[801,848,467,486]" gridcol="2" gridrow="5" pageId="29" pageNumber="188">2.1</td>
<td id="0A1A5DA3FFDF005EFC548D81FC3D02AA" box="[900,938,467,486]" gridcol="3" gridrow="5" pageId="29" pageNumber="188">0.3</td>
<td id="0A1A5DA3FFDF005EFC3B8D81FB9B02AA" box="[1003,1036,467,486]" gridcol="4" gridrow="5" pageId="29" pageNumber="188">0.1</td>
<td id="0A1A5DA3FFDF005EFB988D81FBF902AA" box="[1096,1134,467,486]" gridcol="5" gridrow="5" pageId="29" pageNumber="188">15.3</td>
<td id="0A1A5DA3FFDF005EFB778D81FB4F02AA" box="[1191,1240,467,486]" gridcol="6" gridrow="5" pageId="29" pageNumber="188">1.4</td>
<td id="0A1A5DA3FFDF005EFAD98D81FAAE02AA" box="[1289,1337,467,486]" gridcol="7" gridrow="5" pageId="29" pageNumber="188">2.9</td>
<td id="0A1A5DA3FFDF005EFAA58D81FA1D02AA" box="[1397,1418,467,486]" gridcol="8" gridrow="5" pageId="29" pageNumber="188">21</td>
</tr>
<tr id="49CB34DFFFDF005EFFAD8DAAFA1D0140" box="[125,1418,504,524]" gridrow="6" pageId="29" pageNumber="188">
<th id="0A1A5DA3FFDF005EFFAD8DAAFDF10140" box="[125,614,504,524]" gridcol="0" gridrow="6" pageId="29" pageNumber="188">Oral bulge, height, µm</th>
<td id="0A1A5DA3FFDF005EFD6F8DAAFD790140" box="[703,750,504,524]" gridcol="1" gridrow="6" pageId="29" pageNumber="188">3.2</td>
<td id="0A1A5DA3FFDF005EFCF18DAAFCC70140" box="[801,848,504,524]" gridcol="2" gridrow="6" pageId="29" pageNumber="188">3.0</td>
<td id="0A1A5DA3FFDF005EFC548DAAFC3D0140" box="[900,938,504,524]" gridcol="3" gridrow="6" pageId="29" pageNumber="188"></td>
<td id="0A1A5DA3FFDF005EFC3B8DAAFB9B0140" box="[1003,1036,504,524]" gridcol="4" gridrow="6" pageId="29" pageNumber="188"></td>
<td id="0A1A5DA3FFDF005EFB988DAAFBF90140" box="[1096,1134,504,524]" gridcol="5" gridrow="6" pageId="29" pageNumber="188"></td>
<td id="0A1A5DA3FFDF005EFB778DAAFB4F0140" box="[1191,1240,504,524]" gridcol="6" gridrow="6" pageId="29" pageNumber="188">2.0</td>
<td id="0A1A5DA3FFDF005EFAD98DAAFAAE0140" box="[1289,1337,504,524]" gridcol="7" gridrow="6" pageId="29" pageNumber="188">4.0</td>
<td id="0A1A5DA3FFDF005EFAA58DAAFA1D0140" box="[1397,1418,504,524]" gridcol="8" gridrow="6" pageId="29" pageNumber="188">21</td>
</tr>
<tr id="49CB34DFFFDF005EFFAD8E4CFA1D017D" box="[125,1418,542,561]" gridrow="7" pageId="29" pageNumber="188">
<th id="0A1A5DA3FFDF005EFFAD8E4CFDF1017D" box="[125,614,542,561]" gridcol="0" gridrow="7" pageId="29" pageNumber="188">Oral bulge, width, µm</th>
<td id="0A1A5DA3FFDF005EFD6F8E4CFD79017D" box="[703,750,542,561]" gridcol="1" gridrow="7" pageId="29" pageNumber="188">5.7</td>
<td id="0A1A5DA3FFDF005EFCF18E4CFCC7017D" box="[801,848,542,561]" gridcol="2" gridrow="7" pageId="29" pageNumber="188">6.0</td>
<td id="0A1A5DA3FFDF005EFC548E4CFC3D017D" box="[900,938,542,561]" gridcol="3" gridrow="7" pageId="29" pageNumber="188">0.7</td>
<td id="0A1A5DA3FFDF005EFC3B8E4CFB9B017D" box="[1003,1036,542,561]" gridcol="4" gridrow="7" pageId="29" pageNumber="188">0.2</td>
<td id="0A1A5DA3FFDF005EFB988E4CFBF9017D" box="[1096,1134,542,561]" gridcol="5" gridrow="7" pageId="29" pageNumber="188">12.6</td>
<td id="0A1A5DA3FFDF005EFB778E4CFB4F017D" box="[1191,1240,542,561]" gridcol="6" gridrow="7" pageId="29" pageNumber="188">5.0</td>
<td id="0A1A5DA3FFDF005EFAD98E4CFAAE017D" box="[1289,1337,542,561]" gridcol="7" gridrow="7" pageId="29" pageNumber="188">7.0</td>
<td id="0A1A5DA3FFDF005EFAA58E4CFA1D017D" box="[1397,1418,542,561]" gridcol="8" gridrow="7" pageId="29" pageNumber="188">21</td>
</tr>
<tr id="49CB34DFFFDF005EFFAD8E11FA1D011B" box="[125,1418,579,599]" gridrow="8" pageId="29" pageNumber="188">
<th id="0A1A5DA3FFDF005EFFAD8E11FDF1011B" box="[125,614,579,599]" gridcol="0" gridrow="8" pageId="29" pageNumber="188">Anterior end to macronucleus, distance, µm</th>
<td id="0A1A5DA3FFDF005EFD6F8E11FD79011B" box="[703,750,579,599]" gridcol="1" gridrow="8" pageId="29" pageNumber="188">39.7</td>
<td id="0A1A5DA3FFDF005EFCF18E11FCC7011B" box="[801,848,579,599]" gridcol="2" gridrow="8" pageId="29" pageNumber="188">39.0</td>
<td id="0A1A5DA3FFDF005EFC548E11FC3D011B" box="[900,938,579,599]" gridcol="3" gridrow="8" pageId="29" pageNumber="188">7.7</td>
<td id="0A1A5DA3FFDF005EFC3B8E11FB9B011B" box="[1003,1036,579,599]" gridcol="4" gridrow="8" pageId="29" pageNumber="188">1.7</td>
<td id="0A1A5DA3FFDF005EFB988E11FBF9011B" box="[1096,1134,579,599]" gridcol="5" gridrow="8" pageId="29" pageNumber="188">19.5</td>
<td id="0A1A5DA3FFDF005EFB778E11FB4F011B" box="[1191,1240,579,599]" gridcol="6" gridrow="8" pageId="29" pageNumber="188">29.0</td>
<td id="0A1A5DA3FFDF005EFAD98E11FAAE011B" box="[1289,1337,579,599]" gridcol="7" gridrow="8" pageId="29" pageNumber="188">58.0</td>
<td id="0A1A5DA3FFDF005EFAA58E11FA1D011B" box="[1397,1418,579,599]" gridcol="8" gridrow="8" pageId="29" pageNumber="188">21</td>
</tr>
<tr id="49CB34DFFFDF005EFFAD8E3BFA1D0130" box="[125,1418,617,636]" gridrow="9" pageId="29" pageNumber="188">
<th id="0A1A5DA3FFDF005EFFAD8E3BFDF10130" box="[125,614,617,636]" gridcol="0" gridrow="9" pageId="29" pageNumber="188">Anterior body end to first excretory pore, distance, µm</th>
<td id="0A1A5DA3FFDF005EFD6F8E3BFD790130" box="[703,750,617,636]" gridcol="1" gridrow="9" pageId="29" pageNumber="188">41.3</td>
<td id="0A1A5DA3FFDF005EFCF18E3BFCC70130" box="[801,848,617,636]" gridcol="2" gridrow="9" pageId="29" pageNumber="188">41.0</td>
<td id="0A1A5DA3FFDF005EFC548E3BFC3D0130" box="[900,938,617,636]" gridcol="3" gridrow="9" pageId="29" pageNumber="188">3.0</td>
<td id="0A1A5DA3FFDF005EFC3B8E3BFB9B0130" box="[1003,1036,617,636]" gridcol="4" gridrow="9" pageId="29" pageNumber="188">0.7</td>
<td id="0A1A5DA3FFDF005EFB988E3BFBF90130" box="[1096,1134,617,636]" gridcol="5" gridrow="9" pageId="29" pageNumber="188">7.3</td>
<td id="0A1A5DA3FFDF005EFB778E3BFB4F0130" box="[1191,1240,617,636]" gridcol="6" gridrow="9" pageId="29" pageNumber="188">36.0</td>
<td id="0A1A5DA3FFDF005EFAD98E3BFAAE0130" box="[1289,1337,617,636]" gridcol="7" gridrow="9" pageId="29" pageNumber="188">47.0</td>
<td id="0A1A5DA3FFDF005EFAA58E3BFA1D0130" box="[1397,1418,617,636]" gridcol="8" gridrow="9" pageId="29" pageNumber="188">21</td>
</tr>
<tr id="49CB34DFFFDF005EFFAD8EDCFA1D01EE" box="[125,1418,654,674]" gridrow="10" pageId="29" pageNumber="188">
<th id="0A1A5DA3FFDF005EFFAD8EDCFDF101EE" box="[125,614,654,674]" gridcol="0" gridrow="10" pageId="29" pageNumber="188">Circumoral kinety to end of brush row 1, distance, µm</th>
<td id="0A1A5DA3FFDF005EFD6F8EDCFD7901EE" box="[703,750,654,674]" gridcol="1" gridrow="10" pageId="29" pageNumber="188">20.3</td>
<td id="0A1A5DA3FFDF005EFCF18EDCFCC701EE" box="[801,848,654,674]" gridcol="2" gridrow="10" pageId="29" pageNumber="188">21.0</td>
<td id="0A1A5DA3FFDF005EFC548EDCFC3D01EE" box="[900,938,654,674]" gridcol="3" gridrow="10" pageId="29" pageNumber="188">2.3</td>
<td id="0A1A5DA3FFDF005EFC3B8EDCFB9B01EE" box="[1003,1036,654,674]" gridcol="4" gridrow="10" pageId="29" pageNumber="188">0.5</td>
<td id="0A1A5DA3FFDF005EFB988EDCFBF901EE" box="[1096,1134,654,674]" gridcol="5" gridrow="10" pageId="29" pageNumber="188">11.4</td>
<td id="0A1A5DA3FFDF005EFB778EDCFB4F01EE" box="[1191,1240,654,674]" gridcol="6" gridrow="10" pageId="29" pageNumber="188">16.0</td>
<td id="0A1A5DA3FFDF005EFAD98EDCFAAE01EE" box="[1289,1337,654,674]" gridcol="7" gridrow="10" pageId="29" pageNumber="188">24.0</td>
<td id="0A1A5DA3FFDF005EFAA58EDCFA1D01EE" box="[1397,1418,654,674]" gridcol="8" gridrow="10" pageId="29" pageNumber="188">21</td>
</tr>
<tr id="49CB34DFFFDF005EFFAD8EE1FA1D018B" box="[125,1418,691,711]" gridrow="11" pageId="29" pageNumber="188">
<th id="0A1A5DA3FFDF005EFFAD8EE1FDF1018B" box="[125,614,691,711]" gridcol="0" gridrow="11" pageId="29" pageNumber="188">Circumoral kinety to end of brush row 2, distance, µm</th>
<td id="0A1A5DA3FFDF005EFD6F8EE1FD79018B" box="[703,750,691,711]" gridcol="1" gridrow="11" pageId="29" pageNumber="188">22.5</td>
<td id="0A1A5DA3FFDF005EFCF18EE1FCC7018B" box="[801,848,691,711]" gridcol="2" gridrow="11" pageId="29" pageNumber="188">23.0</td>
<td id="0A1A5DA3FFDF005EFC548EE1FC3D018B" box="[900,938,691,711]" gridcol="3" gridrow="11" pageId="29" pageNumber="188">2.6</td>
<td id="0A1A5DA3FFDF005EFC3B8EE1FB9B018B" box="[1003,1036,691,711]" gridcol="4" gridrow="11" pageId="29" pageNumber="188">0.6</td>
<td id="0A1A5DA3FFDF005EFB988EE1FBF9018B" box="[1096,1134,691,711]" gridcol="5" gridrow="11" pageId="29" pageNumber="188">11.7</td>
<td id="0A1A5DA3FFDF005EFB778EE1FB4F018B" box="[1191,1240,691,711]" gridcol="6" gridrow="11" pageId="29" pageNumber="188">17.0</td>
<td id="0A1A5DA3FFDF005EFAD98EE1FAAE018B" box="[1289,1337,691,711]" gridcol="7" gridrow="11" pageId="29" pageNumber="188">27.0</td>
<td id="0A1A5DA3FFDF005EFAA58EE1FA1D018B" box="[1397,1418,691,711]" gridcol="8" gridrow="11" pageId="29" pageNumber="188">21</td>
</tr>
<tr id="49CB34DFFFDF005EFFAD8E8BFA1D01A0" box="[125,1418,729,748]" gridrow="12" pageId="29" pageNumber="188">
<th id="0A1A5DA3FFDF005EFFAD8E8BFDF101A0" box="[125,614,729,748]" gridcol="0" gridrow="12" pageId="29" pageNumber="188">Circumoral kinety to end of brush row 3, distance, µm</th>
<td id="0A1A5DA3FFDF005EFD6F8E8BFD7901A0" box="[703,750,729,748]" gridcol="1" gridrow="12" pageId="29" pageNumber="188">19.4</td>
<td id="0A1A5DA3FFDF005EFCF18E8BFCC701A0" box="[801,848,729,748]" gridcol="2" gridrow="12" pageId="29" pageNumber="188">19.0</td>
<td id="0A1A5DA3FFDF005EFC548E8BFC3D01A0" box="[900,938,729,748]" gridcol="3" gridrow="12" pageId="29" pageNumber="188">2.8</td>
<td id="0A1A5DA3FFDF005EFC3B8E8BFB9B01A0" box="[1003,1036,729,748]" gridcol="4" gridrow="12" pageId="29" pageNumber="188">0.6</td>
<td id="0A1A5DA3FFDF005EFB988E8BFBF901A0" box="[1096,1134,729,748]" gridcol="5" gridrow="12" pageId="29" pageNumber="188">14.4</td>
<td id="0A1A5DA3FFDF005EFB778E8BFB4F01A0" box="[1191,1240,729,748]" gridcol="6" gridrow="12" pageId="29" pageNumber="188">15.0</td>
<td id="0A1A5DA3FFDF005EFAD98E8BFAAE01A0" box="[1289,1337,729,748]" gridcol="7" gridrow="12" pageId="29" pageNumber="188">26.0</td>
<td id="0A1A5DA3FFDF005EFAA58E8BFA1D01A0" box="[1397,1418,729,748]" gridcol="8" gridrow="12" pageId="29" pageNumber="188">21</td>
</tr>
<tr id="49CB34DFFFDF005EFFAD8EACFA1D005E" box="[125,1418,766,786]" gridrow="13" pageId="29" pageNumber="188">
<th id="0A1A5DA3FFDF005EFFAD8EACFDF1005E" box="[125,614,766,786]" gridcol="0" gridrow="13" pageId="29" pageNumber="188">Macronucleus figure, length, µm</th>
<td id="0A1A5DA3FFDF005EFD6F8EACFD79005E" box="[703,750,766,786]" gridcol="1" gridrow="13" pageId="29" pageNumber="188">38.4</td>
<td id="0A1A5DA3FFDF005EFCF18EACFCC7005E" box="[801,848,766,786]" gridcol="2" gridrow="13" pageId="29" pageNumber="188">35.0</td>
<td id="0A1A5DA3FFDF005EFC548EACFC3D005E" box="[900,938,766,786]" gridcol="3" gridrow="13" pageId="29" pageNumber="188">10.1</td>
<td id="0A1A5DA3FFDF005EFC3B8EACFB9B005E" box="[1003,1036,766,786]" gridcol="4" gridrow="13" pageId="29" pageNumber="188">2.2</td>
<td id="0A1A5DA3FFDF005EFB988EACFBF9005E" box="[1096,1134,766,786]" gridcol="5" gridrow="13" pageId="29" pageNumber="188">26.2</td>
<td id="0A1A5DA3FFDF005EFB778EACFB4F005E" box="[1191,1240,766,786]" gridcol="6" gridrow="13" pageId="29" pageNumber="188">27.0</td>
<td id="0A1A5DA3FFDF005EFAD98EACFAAE005E" box="[1289,1337,766,786]" gridcol="7" gridrow="13" pageId="29" pageNumber="188">68.0</td>
<td id="0A1A5DA3FFDF005EFAA58EACFA1D005E" box="[1397,1418,766,786]" gridcol="8" gridrow="13" pageId="29" pageNumber="188">21</td>
</tr>
<tr id="49CB34DFFFDF005EFFAD8F76FA1D007B" box="[125,1418,804,823]" gridrow="14" pageId="29" pageNumber="188">
<th id="0A1A5DA3FFDF005EFFAD8F76FDF1007B" box="[125,614,804,823]" gridcol="0" gridrow="14" pageId="29" pageNumber="188">Macronucleus, length extended, µm</th>
<td id="0A1A5DA3FFDF005EFD6F8F76FD79007B" box="[703,750,804,823]" gridcol="1" gridrow="14" pageId="29" pageNumber="188">65.7</td>
<td id="0A1A5DA3FFDF005EFCF18F76FCC7007B" box="[801,848,804,823]" gridcol="2" gridrow="14" pageId="29" pageNumber="188">60.0</td>
<td id="0A1A5DA3FFDF005EFC548F76FC3D007B" box="[900,938,804,823]" gridcol="3" gridrow="14" pageId="29" pageNumber="188"></td>
<td id="0A1A5DA3FFDF005EFC3B8F76FB9B007B" box="[1003,1036,804,823]" gridcol="4" gridrow="14" pageId="29" pageNumber="188"></td>
<td id="0A1A5DA3FFDF005EFB988F76FBF9007B" box="[1096,1134,804,823]" gridcol="5" gridrow="14" pageId="29" pageNumber="188"></td>
<td id="0A1A5DA3FFDF005EFB778F76FB4F007B" box="[1191,1240,804,823]" gridcol="6" gridrow="14" pageId="29" pageNumber="188">45.0</td>
<td id="0A1A5DA3FFDF005EFAD98F76FAAE007B" box="[1289,1337,804,823]" gridcol="7" gridrow="14" pageId="29" pageNumber="188">105.0</td>
<td id="0A1A5DA3FFDF005EFAA58F76FA1D007B" box="[1397,1418,804,823]" gridcol="8" gridrow="14" pageId="29" pageNumber="188">21</td>
</tr>
<tr id="49CB34DFFFDF005EFFAD8F1BFA1D0011" box="[125,1418,841,861]" gridrow="15" pageId="29" pageNumber="188">
<th id="0A1A5DA3FFDF005EFFAD8F1BFDF10011" box="[125,614,841,861]" gridcol="0" gridrow="15" pageId="29" pageNumber="188">Macronucleus, width in mid, µm</th>
<td id="0A1A5DA3FFDF005EFD6F8F1BFD790011" box="[703,750,841,861]" gridcol="1" gridrow="15" pageId="29" pageNumber="188">5.1</td>
<td id="0A1A5DA3FFDF005EFCF18F1BFCC70011" box="[801,848,841,861]" gridcol="2" gridrow="15" pageId="29" pageNumber="188">5.0</td>
<td id="0A1A5DA3FFDF005EFC548F1BFC3D0011" box="[900,938,841,861]" gridcol="3" gridrow="15" pageId="29" pageNumber="188">0.4</td>
<td id="0A1A5DA3FFDF005EFC3B8F1BFB9B0011" box="[1003,1036,841,861]" gridcol="4" gridrow="15" pageId="29" pageNumber="188">0.1</td>
<td id="0A1A5DA3FFDF005EFB988F1BFBF90011" box="[1096,1134,841,861]" gridcol="5" gridrow="15" pageId="29" pageNumber="188">7.6</td>
<td id="0A1A5DA3FFDF005EFB778F1BFB4F0011" box="[1191,1240,841,861]" gridcol="6" gridrow="15" pageId="29" pageNumber="188">4.0</td>
<td id="0A1A5DA3FFDF005EFAD98F1BFAAE0011" box="[1289,1337,841,861]" gridcol="7" gridrow="15" pageId="29" pageNumber="188">6.0</td>
<td id="0A1A5DA3FFDF005EFAA58F1BFA1D0011" box="[1397,1418,841,861]" gridcol="8" gridrow="15" pageId="29" pageNumber="188">21</td>
</tr>
<tr id="49CB34DFFFDF005EFFAD8F3DFA1D00CE" box="[125,1418,879,898]" gridrow="16" pageId="29" pageNumber="188">
<th id="0A1A5DA3FFDF005EFFAD8F3DFDF100CE" box="[125,614,879,898]" gridcol="0" gridrow="16" pageId="29" pageNumber="188">Macronuclear nodules, number</th>
<td id="0A1A5DA3FFDF005EFD6F8F3DFD7900CE" box="[703,750,879,898]" gridcol="1" gridrow="16" pageId="29" pageNumber="188">8.1</td>
<td id="0A1A5DA3FFDF005EFCF18F3DFCC700CE" box="[801,848,879,898]" gridcol="2" gridrow="16" pageId="29" pageNumber="188">8.0</td>
<td id="0A1A5DA3FFDF005EFC548F3DFC3D00CE" box="[900,938,879,898]" gridcol="3" gridrow="16" pageId="29" pageNumber="188">1.4</td>
<td id="0A1A5DA3FFDF005EFC3B8F3DFB9B00CE" box="[1003,1036,879,898]" gridcol="4" gridrow="16" pageId="29" pageNumber="188">0.3</td>
<td id="0A1A5DA3FFDF005EFB988F3DFBF900CE" box="[1096,1134,879,898]" gridcol="5" gridrow="16" pageId="29" pageNumber="188">17.1</td>
<td id="0A1A5DA3FFDF005EFB778F3DFB4F00CE" box="[1191,1240,879,898]" gridcol="6" gridrow="16" pageId="29" pageNumber="188">5.0</td>
<td id="0A1A5DA3FFDF005EFAD98F3DFAAE00CE" box="[1289,1337,879,898]" gridcol="7" gridrow="16" pageId="29" pageNumber="188">11.0</td>
<td id="0A1A5DA3FFDF005EFAA58F3DFA1D00CE" box="[1397,1418,879,898]" gridcol="8" gridrow="16" pageId="29" pageNumber="188">21</td>
</tr>
<tr id="49CB34DFFFDF005EFFAD8FC6FA1D00E4" box="[125,1418,916,936]" gridrow="17" pageId="29" pageNumber="188">
<th id="0A1A5DA3FFDF005EFFAD8FC6FDF100E4" box="[125,614,916,936]" gridcol="0" gridrow="17" pageId="29" pageNumber="188">Micronuclei, largest diameter, µm</th>
<td id="0A1A5DA3FFDF005EFD6F8FC6FD7900E4" box="[703,750,916,936]" gridcol="1" gridrow="17" pageId="29" pageNumber="188">2.3</td>
<td id="0A1A5DA3FFDF005EFCF18FC6FCC700E4" box="[801,848,916,936]" gridcol="2" gridrow="17" pageId="29" pageNumber="188">2.2</td>
<td id="0A1A5DA3FFDF005EFC548FC6FC3D00E4" box="[900,938,916,936]" gridcol="3" gridrow="17" pageId="29" pageNumber="188"></td>
<td id="0A1A5DA3FFDF005EFC3B8FC6FB9B00E4" box="[1003,1036,916,936]" gridcol="4" gridrow="17" pageId="29" pageNumber="188"></td>
<td id="0A1A5DA3FFDF005EFB988FC6FBF900E4" box="[1096,1134,916,936]" gridcol="5" gridrow="17" pageId="29" pageNumber="188"></td>
<td id="0A1A5DA3FFDF005EFB778FC6FB4F00E4" box="[1191,1240,916,936]" gridcol="6" gridrow="17" pageId="29" pageNumber="188">2.0</td>
<td id="0A1A5DA3FFDF005EFAD98FC6FAAE00E4" box="[1289,1337,916,936]" gridcol="7" gridrow="17" pageId="29" pageNumber="188">3.0</td>
<td id="0A1A5DA3FFDF005EFAA58FC6FA1D00E4" box="[1397,1418,916,936]" gridcol="8" gridrow="17" pageId="29" pageNumber="188">21</td>
</tr>
<tr id="49CB34DFFFDF005EFFAD8FE8FA1D0081" box="[125,1418,954,973]" gridrow="18" pageId="29" pageNumber="188">
<th id="0A1A5DA3FFDF005EFFAD8FE8FDF10081" box="[125,614,954,973]" gridcol="0" gridrow="18" pageId="29" pageNumber="188">Micronuclei, number</th>
<td id="0A1A5DA3FFDF005EFD6F8FE8FD790081" box="[703,750,954,973]" gridcol="1" gridrow="18" pageId="29" pageNumber="188">8.6</td>
<td id="0A1A5DA3FFDF005EFCF18FE8FCC70081" box="[801,848,954,973]" gridcol="2" gridrow="18" pageId="29" pageNumber="188">8.0</td>
<td id="0A1A5DA3FFDF005EFC548FE8FC3D0081" box="[900,938,954,973]" gridcol="3" gridrow="18" pageId="29" pageNumber="188">2.8</td>
<td id="0A1A5DA3FFDF005EFC3B8FE8FB9B0081" box="[1003,1036,954,973]" gridcol="4" gridrow="18" pageId="29" pageNumber="188">0.6</td>
<td id="0A1A5DA3FFDF005EFB988FE8FBF90081" box="[1096,1134,954,973]" gridcol="5" gridrow="18" pageId="29" pageNumber="188">31.9</td>
<td id="0A1A5DA3FFDF005EFB778FE8FB4F0081" box="[1191,1240,954,973]" gridcol="6" gridrow="18" pageId="29" pageNumber="188">6.0</td>
<td id="0A1A5DA3FFDF005EFAD98FE8FAAE0081" box="[1289,1337,954,973]" gridcol="7" gridrow="18" pageId="29" pageNumber="188">17.0</td>
<td id="0A1A5DA3FFDF005EFAA58FE8FA1D0081" box="[1397,1418,954,973]" gridcol="8" gridrow="18" pageId="29" pageNumber="188">21</td>
</tr>
<tr id="49CB34DFFFDF005EFFAD8F8DFA1D00BF" box="[125,1418,991,1011]" gridrow="19" pageId="29" pageNumber="188">
<th id="0A1A5DA3FFDF005EFFAD8F8DFDF100BF" box="[125,614,991,1011]" gridcol="0" gridrow="19" pageId="29" pageNumber="188">Ciliary rows, number (including brush rows)</th>
<td id="0A1A5DA3FFDF005EFD6F8F8DFD7900BF" box="[703,750,991,1011]" gridcol="1" gridrow="19" pageId="29" pageNumber="188">15.0</td>
<td id="0A1A5DA3FFDF005EFCF18F8DFCC700BF" box="[801,848,991,1011]" gridcol="2" gridrow="19" pageId="29" pageNumber="188">15.0</td>
<td id="0A1A5DA3FFDF005EFC548F8DFC3D00BF" box="[900,938,991,1011]" gridcol="3" gridrow="19" pageId="29" pageNumber="188">1.1</td>
<td id="0A1A5DA3FFDF005EFC3B8F8DFB9B00BF" box="[1003,1036,991,1011]" gridcol="4" gridrow="19" pageId="29" pageNumber="188">0.2</td>
<td id="0A1A5DA3FFDF005EFB988F8DFBF900BF" box="[1096,1134,991,1011]" gridcol="5" gridrow="19" pageId="29" pageNumber="188">7.5</td>
<td id="0A1A5DA3FFDF005EFB778F8DFB4F00BF" box="[1191,1240,991,1011]" gridcol="6" gridrow="19" pageId="29" pageNumber="188">13.0</td>
<td id="0A1A5DA3FFDF005EFAD98F8DFAAE00BF" box="[1289,1337,991,1011]" gridcol="7" gridrow="19" pageId="29" pageNumber="188">17.0</td>
<td id="0A1A5DA3FFDF005EFAA58F8DFA1D00BF" box="[1397,1418,991,1011]" gridcol="8" gridrow="19" pageId="29" pageNumber="188">21</td>
</tr>
<tr id="49CB34DFFFDF005EFFAD8857FA1D0754" box="[125,1418,1029,1048]" gridrow="20" pageId="29" pageNumber="188">
<th id="0A1A5DA3FFDF005EFFAD8857FDF10754" box="[125,614,1029,1048]" gridcol="0" gridrow="20" pageId="29" pageNumber="188">Ciliated kinetids in a right side kinety, number</th>
<td id="0A1A5DA3FFDF005EFD6F8857FD790754" box="[703,750,1029,1048]" gridcol="1" gridrow="20" pageId="29" pageNumber="188">41.2</td>
<td id="0A1A5DA3FFDF005EFCF18857FCC70754" box="[801,848,1029,1048]" gridcol="2" gridrow="20" pageId="29" pageNumber="188">40.0</td>
<td id="0A1A5DA3FFDF005EFC548857FC3D0754" box="[900,938,1029,1048]" gridcol="3" gridrow="20" pageId="29" pageNumber="188">8.8</td>
<td id="0A1A5DA3FFDF005EFC3B8857FB9B0754" box="[1003,1036,1029,1048]" gridcol="4" gridrow="20" pageId="29" pageNumber="188">1.9</td>
<td id="0A1A5DA3FFDF005EFB988857FBF90754" box="[1096,1134,1029,1048]" gridcol="5" gridrow="20" pageId="29" pageNumber="188">21.3</td>
<td id="0A1A5DA3FFDF005EFB778857FB4F0754" box="[1191,1240,1029,1048]" gridcol="6" gridrow="20" pageId="29" pageNumber="188">23.0</td>
<td id="0A1A5DA3FFDF005EFAD98857FAAE0754" box="[1289,1337,1029,1048]" gridcol="7" gridrow="20" pageId="29" pageNumber="188">65.0</td>
<td id="0A1A5DA3FFDF005EFAA58857FA1D0754" box="[1397,1418,1029,1048]" gridcol="8" gridrow="20" pageId="29" pageNumber="188">21</td>
</tr>
<tr id="49CB34DFFFDF005EFFAD8878FA1D0772" box="[125,1418,1066,1086]" gridrow="21" pageId="29" pageNumber="188">
<th id="0A1A5DA3FFDF005EFFAD8878FDF10772" box="[125,614,1066,1086]" gridcol="0" gridrow="21" pageId="29" pageNumber="188">Dorsal brush rows, number</th>
<td id="0A1A5DA3FFDF005EFD6F8878FD790772" box="[703,750,1066,1086]" gridcol="1" gridrow="21" pageId="29" pageNumber="188">3.0</td>
<td id="0A1A5DA3FFDF005EFCF18878FCC70772" box="[801,848,1066,1086]" gridcol="2" gridrow="21" pageId="29" pageNumber="188">3.0</td>
<td id="0A1A5DA3FFDF005EFC548878FC3D0772" box="[900,938,1066,1086]" gridcol="3" gridrow="21" pageId="29" pageNumber="188">0.0</td>
<td id="0A1A5DA3FFDF005EFC3B8878FB9B0772" box="[1003,1036,1066,1086]" gridcol="4" gridrow="21" pageId="29" pageNumber="188">0.0</td>
<td id="0A1A5DA3FFDF005EFB988878FBF90772" box="[1096,1134,1066,1086]" gridcol="5" gridrow="21" pageId="29" pageNumber="188">0.0</td>
<td id="0A1A5DA3FFDF005EFB778878FB4F0772" box="[1191,1240,1066,1086]" gridcol="6" gridrow="21" pageId="29" pageNumber="188">3.0</td>
<td id="0A1A5DA3FFDF005EFAD98878FAAE0772" box="[1289,1337,1066,1086]" gridcol="7" gridrow="21" pageId="29" pageNumber="188">3.0</td>
<td id="0A1A5DA3FFDF005EFAA58878FA1D0772" box="[1397,1418,1066,1086]" gridcol="8" gridrow="21" pageId="29" pageNumber="188">21</td>
</tr>
<tr id="49CB34DFFFDF005EFFAD8802FA1D072F" box="[125,1418,1104,1123]" gridrow="22" pageId="29" pageNumber="188">
<th id="0A1A5DA3FFDF005EFFAD8802FDF1072F" box="[125,614,1104,1123]" gridcol="0" gridrow="22" pageId="29" pageNumber="188">Dikinetids in brush row 1, number</th>
<td id="0A1A5DA3FFDF005EFD6F8802FD79072F" box="[703,750,1104,1123]" gridcol="1" gridrow="22" pageId="29" pageNumber="188">15.5</td>
<td id="0A1A5DA3FFDF005EFCF18802FCC7072F" box="[801,848,1104,1123]" gridcol="2" gridrow="22" pageId="29" pageNumber="188">16.0</td>
<td id="0A1A5DA3FFDF005EFC548802FC3D072F" box="[900,938,1104,1123]" gridcol="3" gridrow="22" pageId="29" pageNumber="188">2.3</td>
<td id="0A1A5DA3FFDF005EFC3B8802FB9B072F" box="[1003,1036,1104,1123]" gridcol="4" gridrow="22" pageId="29" pageNumber="188">0.5</td>
<td id="0A1A5DA3FFDF005EFB988802FBF9072F" box="[1096,1134,1104,1123]" gridcol="5" gridrow="22" pageId="29" pageNumber="188">15.1</td>
<td id="0A1A5DA3FFDF005EFB778802FB4F072F" box="[1191,1240,1104,1123]" gridcol="6" gridrow="22" pageId="29" pageNumber="188">12.0</td>
<td id="0A1A5DA3FFDF005EFAD98802FAAE072F" box="[1289,1337,1104,1123]" gridcol="7" gridrow="22" pageId="29" pageNumber="188">22.0</td>
<td id="0A1A5DA3FFDF005EFAA58802FA1D072F" box="[1397,1418,1104,1123]" gridcol="8" gridrow="22" pageId="29" pageNumber="188">21</td>
</tr>
<tr id="49CB34DFFFDF005EFFAD8827FA1D07C5" box="[125,1418,1141,1161]" gridrow="23" pageId="29" pageNumber="188">
<th id="0A1A5DA3FFDF005EFFAD8827FDF107C5" box="[125,614,1141,1161]" gridcol="0" gridrow="23" pageId="29" pageNumber="188">Dikinetids in brush row 2, number</th>
<td id="0A1A5DA3FFDF005EFD6F8827FD7907C5" box="[703,750,1141,1161]" gridcol="1" gridrow="23" pageId="29" pageNumber="188">18.1</td>
<td id="0A1A5DA3FFDF005EFCF18827FCC707C5" box="[801,848,1141,1161]" gridcol="2" gridrow="23" pageId="29" pageNumber="188">19.0</td>
<td id="0A1A5DA3FFDF005EFC548827FC3D07C5" box="[900,938,1141,1161]" gridcol="3" gridrow="23" pageId="29" pageNumber="188">2.7</td>
<td id="0A1A5DA3FFDF005EFC3B8827FB9B07C5" box="[1003,1036,1141,1161]" gridcol="4" gridrow="23" pageId="29" pageNumber="188">0.6</td>
<td id="0A1A5DA3FFDF005EFB988827FBF907C5" box="[1096,1134,1141,1161]" gridcol="5" gridrow="23" pageId="29" pageNumber="188">14.8</td>
<td id="0A1A5DA3FFDF005EFB778827FB4F07C5" box="[1191,1240,1141,1161]" gridcol="6" gridrow="23" pageId="29" pageNumber="188">12.0</td>
<td id="0A1A5DA3FFDF005EFAD98827FAAE07C5" box="[1289,1337,1141,1161]" gridcol="7" gridrow="23" pageId="29" pageNumber="188">22.0</td>
<td id="0A1A5DA3FFDF005EFAA58827FA1D07C5" box="[1397,1418,1141,1161]" gridcol="8" gridrow="23" pageId="29" pageNumber="188">21</td>
</tr>
<tr id="49CB34DFFFDF005EFFAD88C9FA1D07E2" box="[125,1418,1179,1198]" gridrow="24" pageId="29" pageNumber="188">
<th id="0A1A5DA3FFDF005EFFAD88C9FDF107E2" box="[125,614,1179,1198]" gridcol="0" gridrow="24" pageId="29" pageNumber="188">Dikinetids in brush row 3, number</th>
<td id="0A1A5DA3FFDF005EFD6F88C9FD7907E2" box="[703,750,1179,1198]" gridcol="1" gridrow="24" pageId="29" pageNumber="188">14.1</td>
<td id="0A1A5DA3FFDF005EFCF188C9FCC707E2" box="[801,848,1179,1198]" gridcol="2" gridrow="24" pageId="29" pageNumber="188">14.0</td>
<td id="0A1A5DA3FFDF005EFC5488C9FC3D07E2" box="[900,938,1179,1198]" gridcol="3" gridrow="24" pageId="29" pageNumber="188">1.9</td>
<td id="0A1A5DA3FFDF005EFC3B88C9FB9B07E2" box="[1003,1036,1179,1198]" gridcol="4" gridrow="24" pageId="29" pageNumber="188">0.4</td>
<td id="0A1A5DA3FFDF005EFB9888C9FBF907E2" box="[1096,1134,1179,1198]" gridcol="5" gridrow="24" pageId="29" pageNumber="188">13.3</td>
<td id="0A1A5DA3FFDF005EFB7788C9FB4F07E2" box="[1191,1240,1179,1198]" gridcol="6" gridrow="24" pageId="29" pageNumber="188">11.0</td>
<td id="0A1A5DA3FFDF005EFAD988C9FAAE07E2" box="[1289,1337,1179,1198]" gridcol="7" gridrow="24" pageId="29" pageNumber="188">19.0</td>
<td id="0A1A5DA3FFDF005EFAA588C9FA1D07E2" box="[1397,1418,1179,1198]" gridcol="8" gridrow="24" pageId="29" pageNumber="188">21</td>
</tr>
<tr id="49CB34DFFFDF005EFFAD8892FA1D0798" box="[125,1418,1216,1236]" gridrow="25" pageId="29" pageNumber="188">
<th id="0A1A5DA3FFDF005EFFAD8892FDF10798" box="[125,614,1216,1236]" gridcol="0" gridrow="25" pageId="29" pageNumber="188">Excretory pores for anterior contractile vacuole, number</th>
<td id="0A1A5DA3FFDF005EFD6F8892FD790798" box="[703,750,1216,1236]" gridcol="1" gridrow="25" pageId="29" pageNumber="188">2.6</td>
<td id="0A1A5DA3FFDF005EFCF18892FCC70798" box="[801,848,1216,1236]" gridcol="2" gridrow="25" pageId="29" pageNumber="188">3.0</td>
<td id="0A1A5DA3FFDF005EFC548892FC3D0798" box="[900,938,1216,1236]" gridcol="3" gridrow="25" pageId="29" pageNumber="188"></td>
<td id="0A1A5DA3FFDF005EFC3B8892FB9B0798" box="[1003,1036,1216,1236]" gridcol="4" gridrow="25" pageId="29" pageNumber="188"></td>
<td id="0A1A5DA3FFDF005EFB988892FBF90798" box="[1096,1134,1216,1236]" gridcol="5" gridrow="25" pageId="29" pageNumber="188"></td>
<td id="0A1A5DA3FFDF005EFB778892FB4F0798" box="[1191,1240,1216,1236]" gridcol="6" gridrow="25" pageId="29" pageNumber="188">2.0</td>
<td id="0A1A5DA3FFDF005EFAD98892FAAE0798" box="[1289,1337,1216,1236]" gridcol="7" gridrow="25" pageId="29" pageNumber="188">3.0</td>
<td id="0A1A5DA3FFDF005EFAA58892FA1D0798" box="[1397,1418,1216,1236]" gridcol="8" gridrow="25" pageId="29" pageNumber="188">21</td>
</tr>
<tr id="49CB34DFFFDF005EFFAD88B4FA1D07B5" box="[125,1418,1254,1273]" gridrow="26" pageId="29" pageNumber="188">
<th id="0A1A5DA3FFDF005EFFAD88B4FDF107B5" box="[125,614,1254,1273]" gridcol="0" gridrow="26" pageId="29" pageNumber="188">Excretory pores for posterior contractile vacuole, number</th>
<td id="0A1A5DA3FFDF005EFD6F88B4FD7907B5" box="[703,750,1254,1273]" gridcol="1" gridrow="26" pageId="29" pageNumber="188">2.9</td>
<td id="0A1A5DA3FFDF005EFCF188B4FCC707B5" box="[801,848,1254,1273]" gridcol="2" gridrow="26" pageId="29" pageNumber="188">3.0</td>
<td id="0A1A5DA3FFDF005EFC5488B4FC3D07B5" box="[900,938,1254,1273]" gridcol="3" gridrow="26" pageId="29" pageNumber="188"></td>
<td id="0A1A5DA3FFDF005EFC3B88B4FB9B07B5" box="[1003,1036,1254,1273]" gridcol="4" gridrow="26" pageId="29" pageNumber="188"></td>
<td id="0A1A5DA3FFDF005EFB9888B4FBF907B5" box="[1096,1134,1254,1273]" gridcol="5" gridrow="26" pageId="29" pageNumber="188"></td>
<td id="0A1A5DA3FFDF005EFB7788B4FB4F07B5" box="[1191,1240,1254,1273]" gridcol="6" gridrow="26" pageId="29" pageNumber="188">2.0</td>
<td id="0A1A5DA3FFDF005EFAD988B4FAAE07B5" box="[1289,1337,1254,1273]" gridcol="7" gridrow="26" pageId="29" pageNumber="188">4.0</td>
<td id="0A1A5DA3FFDF005EFAA588B4FA1D07B5" box="[1397,1418,1254,1273]" gridcol="8" gridrow="26" pageId="29" pageNumber="188">21</td>
</tr>
</table>
</paragraph>
<paragraph id="F744369DFFDFFFBCFFAD895DFEBC0615" blockId="29.[125,1456,1295,1370]" pageId="29" pageNumber="188">
<tableNote id="0A1D3713FFDFFFBCFFAD895DFEBC0615" pageId="29" pageNumber="188" targetBox="[125,1418,276,1273]" targetPageId="29">
<superScript id="008E9BD5FFDFFFBCFFAD895DFF140657" attach="left" box="[125,131,1295,1307]" fontSize="5" pageId="29" pageNumber="188">a</superScript>
Data based on mounted, protargol-impregnated (Foissners method), and randomly selected specimens from a raw culture. CV coefficient of variation in %, M median, Max maximum, Min minimum, n number of individuals investigated, SD standard deviation, SE standard error of arithmetic mean,
<emphasis id="C58FEA8FFFDFFFBCFFAD8915FF100616" box="[125,135,1351,1370]" italics="true" pageId="29" pageNumber="188"></emphasis>
arithmetic mean.
</tableNote>
</paragraph>
<paragraph id="F744369DFFDFFFBCFF738A32FF5C0449" blockId="29.[125,773,1493,1936]" pageId="29" pageNumber="188">
<emphasis id="C58FEA8FFFDFFFBCFF738A32FEA40536" bold="true" box="[163,307,1632,1658]" pageId="29" pageNumber="188">Dedication:</emphasis>
The senior author dedicates this new species to Dr. rer. nat. habil. Klaus W. Wolf, Electron Microscopy Unit at the University of the West Indies,
<collectingRegion id="353FF87FFFDFFFBCFFAF8A9AFF7905AE" box="[127,238,1736,1762]" country="Jamaica" name="Kingston" pageId="29" pageNumber="188">Kingston</collectingRegion>
,
<collectingCountry id="8FEC760DFFDFFFBCFF2F8A9AFEF605AE" box="[255,353,1736,1762]" name="Jamaica" pageId="29" pageNumber="188">Jamaica</collectingCountry>
, who facilitated collection on the island.
</paragraph>
</subSubSection>
<subSubSection id="BFE16516FFDFFF83FF738B5FFE98011F" lastPageId="34" lastPageNumber="193" pageId="29" pageNumber="188" type="description">
<paragraph id="F744369DFFDFFF81FF738B5FFD43069E" blockId="29.[125,773,1493,1936]" lastBlockId="32.[131,781,1221,1941]" lastPageId="32" lastPageNumber="191" pageId="29" pageNumber="188">
<emphasis id="C58FEA8FFFDFFFBCFF738B5FFEAA046B" bold="true" box="[163,317,1805,1831]" pageId="29" pageNumber="188">Description:</emphasis>
Size 100160 × 1835 µm
<emphasis id="C58FEA8FFFDFFFBCFD778B5CFD6B046B" box="[679,764,1806,1831]" italics="true" pageId="29" pageNumber="188">in vivo</emphasis>
, usually near 135 × 25 µm. Narrowly to very narrowly spatulate to bluntly fusiform, especially in preparations containing many specimens with inflated middle body third; length:width ratio thus rather different
<emphasis id="C58FEA8FFFDFFFBCFA8A8984FA3806A3" box="[1370,1455,1494,1519]" italics="true" pageId="29" pageNumber="188">in vivo</emphasis>
(~ 5.5:1) and preparations (~ 4:1). Neck indistinct, widest usually in middle body third, body ends up to 2:1 flattened. Anterior (oral) end oblique, posterior narrowly rounded (
<figureCitation id="6FC02A18FFDFFFBCFC678A32FBAD0537" box="[951,1082,1632,1659]" captionStart="Figs 19" captionStartId="30.[132,175,1353,1375]" captionTargetBox="[135,1457,228,1289]" captionTargetId="figure-7@30.[132,1457,223,1309]" captionTargetPageId="30" captionText="Figs 19al. Spathidium wolfi from life (ah) and after protargol impregnation (il). a right side view of a representative specimen, length 140 µm. The arrowhead marks the anterior contractile vacuole; b frontal view of oral bulge; c oral bulge extrusomes, length 10 µm; d developing extrusomes in the cytoplasm; e slender shape variant, showing the two contractile vacuoles (arrowheads); f development of the “Ringgranula” (cp. Fig. 19a); g, h surface view and optical section, showing the cortical granulation; i, j ventral and dorsal view of anterior body region of a paratype specimen, showing the isostichad dorsal brush and the cuneate oral bulge; k, l right and left side view of anterior body region of the holotype specimen (cp. Figs 20a, b). B(13) dorsal brush rows, BA oral basket, BU oral bulge, CK circumoral kinety, E extrusomes, F oral bulge fibres, G cortical granules, L lipid droplet, MA macronucleus, RG “Ringgranula”. Scale bars: 15 µm (il) and 50 µm (a)." figureDoi="http://doi.org/10.5281/zenodo.10371178" httpUri="https://zenodo.org/record/10371178/files/figure.png" pageId="29" pageNumber="188">Figs 19a, e</figureCitation>
,
<figureCitation id="6FC02A18FFDFFFBCFB978A32FB3B0537" box="[1095,1196,1632,1659]" captionStart="Figs 20" captionStartId="31.[125,168,1640,1662]" captionTargetBox="[146,1430,226,1570]" captionTargetId="figure-8@31.[125,1450,223,1587]" captionTargetPageId="31" captionText="Figs 20af. Spathidium wolfi from life (c) and after protargol impregnation (a, b, df). a, b ciliary pattern of right and left side and nuclear apparatus of holotype specimen; for oral details, see Figs 19k, l. The arrowhead marks the excretory pores of the anterior contractile vacu- ole, i.e., the main character of this species; c supposed structure of dorsal brush row 3; d ventral view of a paratype specimen with open circumoral kinety (arrow) and thus resembling Apertospathula; e dorsolateral view of a paratype specimen, showing the ciliary pattern and the excretory pores of the anterior and posterior contractile vacuole; f ventrolateral view of oral body region, showing the Spathidium ciliary pattern. B(13) dorsal brush (rows), BU oral bulge, CK circumoral kinety, CV contractile vacuole, EP excretory pores, MA macronucleus, MI micronuclei. Scale bars: 10 µm (d, f) and 50 µm (a, b, e)." figureDoi="http://doi.org/10.5281/zenodo.10371180" httpUri="https://zenodo.org/record/10371180/files/figure.png" pageId="29" pageNumber="188">20a, b, e</figureCitation>
,
<figureCitation id="6FC02A18FFDFFFBCFB698A32FA930536" box="[1209,1284,1632,1658]" captionStart="Figs 22" captionStartId="33.[125,168,1750,1772]" captionTargetBox="[125,1450,228,1713]" captionTargetId="figure-8@33.[125,1450,223,1725]" captionTargetPageId="33" captionText="Figs 22ak. Spathidium wolfi after protargol impregnation. a a slender specimen; b overview of a specimen inflated by the prepara- tion; c, d overviews showing the moniliform macronucleus; eg ventral views, showing the obovate (or broadly cuneate) circumoral kinety; h ventrolateral view, showing kineties and cortical granules; ik anterior body portion at three focal planes. (i) Right side surface view, showing fibres in oral bulge and somatic cortex (arrowheads). (j) When focused slightly deeper, the nematodesmata become visible. (k) When focused to the left side, the dorsal brush and the Spathidium ciliary pattern become recognizable. B(13) dorsal brush (rows), BU oral bulge, CK circumoral kinety, E extrusomes, EP excretory pores, F fibres, G cortical granules, K kineties, MA mac- ronucleus, MI micronuclei, N nematodesmata. Scale bars: 10 µm (ek) and 50 µm (ad)." figureDoi="http://doi.org/10.5281/zenodo.10371186" httpUri="https://zenodo.org/record/10371186/files/figure.png" pageId="29" pageNumber="188">22ad</figureCitation>
;
<tableCitation id="BA790326FFDFFFBCFADF8A32FAFF0536" box="[1295,1384,1632,1658]" captionStart="Table 7" captionStartId="29.[125,181,223,244]" captionText="Table 7. Morphometric data on Spathidium wolfi." pageId="29" pageNumber="188">Table 7</tableCitation>
). Macronucleus in middle body third, moniliform assuming various figures ranging from cylindroidal to circular, composed of an average of eight nodules connected by narrow bridges; individual nodules globular to elongate ellipsoidal, contain many small nucleoli. Micronuclei near and attached to macronucleus, inconspicuous, that is, about 2 µm across in protargol preparations (
<figureCitation id="6FC02A18FFDFFFBCFAAB8B01FCC204C3" captionStart="Figs 19" captionStartId="30.[132,175,1353,1375]" captionTargetBox="[135,1457,228,1289]" captionTargetId="figure-7@30.[132,1457,223,1309]" captionTargetPageId="30" captionText="Figs 19al. Spathidium wolfi from life (ah) and after protargol impregnation (il). a right side view of a representative specimen, length 140 µm. The arrowhead marks the anterior contractile vacuole; b frontal view of oral bulge; c oral bulge extrusomes, length 10 µm; d developing extrusomes in the cytoplasm; e slender shape variant, showing the two contractile vacuoles (arrowheads); f development of the “Ringgranula” (cp. Fig. 19a); g, h surface view and optical section, showing the cortical granulation; i, j ventral and dorsal view of anterior body region of a paratype specimen, showing the isostichad dorsal brush and the cuneate oral bulge; k, l right and left side view of anterior body region of the holotype specimen (cp. Figs 20a, b). B(13) dorsal brush rows, BA oral basket, BU oral bulge, CK circumoral kinety, E extrusomes, F oral bulge fibres, G cortical granules, L lipid droplet, MA macronucleus, RG “Ringgranula”. Scale bars: 15 µm (il) and 50 µm (a)." figureDoi="http://doi.org/10.5281/zenodo.10371178" httpUri="https://zenodo.org/record/10371178/files/figure.png" pageId="29" pageNumber="188">Figs 19a</figureCitation>
,
<figureCitation id="6FC02A18FFDFFFBCFCB18B27FC3F04DC" box="[865,936,1909,1936]" captionStart="Figs 20" captionStartId="31.[125,168,1640,1662]" captionTargetBox="[146,1430,226,1570]" captionTargetId="figure-8@31.[125,1450,223,1587]" captionTargetPageId="31" captionText="Figs 20af. Spathidium wolfi from life (c) and after protargol impregnation (a, b, df). a, b ciliary pattern of right and left side and nuclear apparatus of holotype specimen; for oral details, see Figs 19k, l. The arrowhead marks the excretory pores of the anterior contractile vacu- ole, i.e., the main character of this species; c supposed structure of dorsal brush row 3; d ventral view of a paratype specimen with open circumoral kinety (arrow) and thus resembling Apertospathula; e dorsolateral view of a paratype specimen, showing the ciliary pattern and the excretory pores of the anterior and posterior contractile vacuole; f ventrolateral view of oral body region, showing the Spathidium ciliary pattern. B(13) dorsal brush (rows), BU oral bulge, CK circumoral kinety, CV contractile vacuole, EP excretory pores, MA macronucleus, MI micronuclei. Scale bars: 10 µm (d, f) and 50 µm (a, b, e)." figureDoi="http://doi.org/10.5281/zenodo.10371180" httpUri="https://zenodo.org/record/10371180/files/figure.png" pageId="29" pageNumber="188">20a, e</figureCitation>
,
<figureCitation id="6FC02A18FFDFFFBCFC648B27FC7404C3" box="[948,995,1909,1935]" captionStart="Figs 21" captionStartId="32.[132,175,1013,1035]" captionTargetBox="[132,1457,223,981]" captionTargetId="figure-7@32.[132,1457,223,990]" captionTargetPageId="32" captionText="Figs 21ac. Spathidium wolfi, dorsal views of anterior body region after protargol impregnation, showing the isostichad dorsal brush (c, arrows) and the excretory pores (arrowheads) of the anterior contractile vacuole. B(13) dorsal brush rows, BU oral bulge, CK cir- cumoral kinety, MA macronucleus. Scale bars: 20 µm." figureDoi="http://doi.org/10.5281/zenodo.10371184" httpUri="https://zenodo.org/record/10371184/files/figure.png" pageId="29" pageNumber="188">21b</figureCitation>
,
<figureCitation id="6FC02A18FFDFFFBCFC3E8B27FBAE04C3" box="[1006,1081,1909,1935]" captionStart="Figs 22" captionStartId="33.[125,168,1750,1772]" captionTargetBox="[125,1450,228,1713]" captionTargetId="figure-8@33.[125,1450,223,1725]" captionTargetPageId="33" captionText="Figs 22ak. Spathidium wolfi after protargol impregnation. a a slender specimen; b overview of a specimen inflated by the prepara- tion; c, d overviews showing the moniliform macronucleus; eg ventral views, showing the obovate (or broadly cuneate) circumoral kinety; h ventrolateral view, showing kineties and cortical granules; ik anterior body portion at three focal planes. (i) Right side surface view, showing fibres in oral bulge and somatic cortex (arrowheads). (j) When focused slightly deeper, the nematodesmata become visible. (k) When focused to the left side, the dorsal brush and the Spathidium ciliary pattern become recognizable. B(13) dorsal brush (rows), BU oral bulge, CK circumoral kinety, E extrusomes, EP excretory pores, F fibres, G cortical granules, K kineties, MA mac- ronucleus, MI micronuclei, N nematodesmata. Scale bars: 10 µm (ek) and 50 µm (ad)." figureDoi="http://doi.org/10.5281/zenodo.10371186" httpUri="https://zenodo.org/record/10371186/files/figure.png" pageId="29" pageNumber="188">22ad</figureCitation>
;
<tableCitation id="BA790326FFDFFFBCFB948B27FB0B04C3" box="[1092,1180,1909,1935]" captionStart="Table 7" captionStartId="29.[125,181,223,244]" captionText="Table 7. Morphometric data on Spathidium wolfi." pageId="29" pageNumber="188">Table 7</tableCitation>
). Two contractile vacuoles, each with adventive blisters during diastole (
<figureCitation id="6FC02A18FFDCFFBFFD068AD7FF45058E" captionStart="Figs 19" captionStartId="30.[132,175,1353,1375]" captionTargetBox="[135,1457,228,1289]" captionTargetId="figure-7@30.[132,1457,223,1309]" captionTargetPageId="30" captionText="Figs 19al. Spathidium wolfi from life (ah) and after protargol impregnation (il). a right side view of a representative specimen, length 140 µm. The arrowhead marks the anterior contractile vacuole; b frontal view of oral bulge; c oral bulge extrusomes, length 10 µm; d developing extrusomes in the cytoplasm; e slender shape variant, showing the two contractile vacuoles (arrowheads); f development of the “Ringgranula” (cp. Fig. 19a); g, h surface view and optical section, showing the cortical granulation; i, j ventral and dorsal view of anterior body region of a paratype specimen, showing the isostichad dorsal brush and the cuneate oral bulge; k, l right and left side view of anterior body region of the holotype specimen (cp. Figs 20a, b). B(13) dorsal brush rows, BA oral basket, BU oral bulge, CK circumoral kinety, E extrusomes, F oral bulge fibres, G cortical granules, L lipid droplet, MA macronucleus, RG “Ringgranula”. Scale bars: 15 µm (il) and 50 µm (a)." figureDoi="http://doi.org/10.5281/zenodo.10371178" httpUri="https://zenodo.org/record/10371178/files/figure.png" pageId="30" pageNumber="189">Figs 19a, e</figureCitation>
,
<figureCitation id="6FC02A18FFDCFFBFFF338AFAFEB8058E" box="[227,303,1704,1730]" captionStart="Figs 20" captionStartId="31.[125,168,1640,1662]" captionTargetBox="[146,1430,226,1570]" captionTargetId="figure-8@31.[125,1450,223,1587]" captionTargetPageId="31" captionText="Figs 20af. Spathidium wolfi from life (c) and after protargol impregnation (a, b, df). a, b ciliary pattern of right and left side and nuclear apparatus of holotype specimen; for oral details, see Figs 19k, l. The arrowhead marks the excretory pores of the anterior contractile vacu- ole, i.e., the main character of this species; c supposed structure of dorsal brush row 3; d ventral view of a paratype specimen with open circumoral kinety (arrow) and thus resembling Apertospathula; e dorsolateral view of a paratype specimen, showing the ciliary pattern and the excretory pores of the anterior and posterior contractile vacuole; f ventrolateral view of oral body region, showing the Spathidium ciliary pattern. B(13) dorsal brush (rows), BU oral bulge, CK circumoral kinety, CV contractile vacuole, EP excretory pores, MA macronucleus, MI micronuclei. Scale bars: 10 µm (d, f) and 50 µm (a, b, e)." figureDoi="http://doi.org/10.5281/zenodo.10371180" httpUri="https://zenodo.org/record/10371180/files/figure.png" pageId="30" pageNumber="189">20a, e</figureCitation>
,
<figureCitation id="6FC02A18FFDCFFBFFE908AFAFE19058E" box="[320,398,1704,1730]" captionStart="Figs 21" captionStartId="32.[132,175,1013,1035]" captionTargetBox="[132,1457,223,981]" captionTargetId="figure-7@32.[132,1457,223,990]" captionTargetPageId="32" captionText="Figs 21ac. Spathidium wolfi, dorsal views of anterior body region after protargol impregnation, showing the isostichad dorsal brush (c, arrows) and the excretory pores (arrowheads) of the anterior contractile vacuole. B(13) dorsal brush rows, BU oral bulge, CK cir- cumoral kinety, MA macronucleus. Scale bars: 20 µm." figureDoi="http://doi.org/10.5281/zenodo.10371184" httpUri="https://zenodo.org/record/10371184/files/figure.png" pageId="30" pageNumber="189">21a, b</figureCitation>
,
<figureCitation id="6FC02A18FFDCFFBFFE4F8AFAFE79058E" box="[415,494,1704,1730]" captionStart="Figs 22" captionStartId="33.[125,168,1750,1772]" captionTargetBox="[125,1450,228,1713]" captionTargetId="figure-8@33.[125,1450,223,1725]" captionTargetPageId="33" captionText="Figs 22ak. Spathidium wolfi after protargol impregnation. a a slender specimen; b overview of a specimen inflated by the prepara- tion; c, d overviews showing the moniliform macronucleus; eg ventral views, showing the obovate (or broadly cuneate) circumoral kinety; h ventrolateral view, showing kineties and cortical granules; ik anterior body portion at three focal planes. (i) Right side surface view, showing fibres in oral bulge and somatic cortex (arrowheads). (j) When focused slightly deeper, the nematodesmata become visible. (k) When focused to the left side, the dorsal brush and the Spathidium ciliary pattern become recognizable. B(13) dorsal brush (rows), BU oral bulge, CK circumoral kinety, E extrusomes, EP excretory pores, F fibres, G cortical granules, K kineties, MA mac- ronucleus, MI micronuclei, N nematodesmata. Scale bars: 10 µm (ek) and 50 µm (ad)." figureDoi="http://doi.org/10.5281/zenodo.10371186" httpUri="https://zenodo.org/record/10371186/files/figure.png" pageId="30" pageNumber="189">22b, c</figureCitation>
;
<tableCitation id="BA790326FFDCFFBFFE2E8AFAFDCA058E" box="[510,605,1704,1730]" captionStart="Table 7" captionStartId="29.[125,181,223,244]" captionText="Table 7. Morphometric data on Spathidium wolfi." pageId="30" pageNumber="189">Table 7</tableCitation>
): one dorsally at margin of first and second body third, the other in rear end, as usual. Anterior vacuole with an average of three serially arranged excretory pores invariably locat- ed between kineties differentiated to dorsal brush rows 2 and 3 anteriorly. Extrusomes studded in oral bulge and scattered in cytoplasm, where intensely impregnated, fusiform developmental stages occur (
<figureCitation id="6FC02A18FFDCFFBFFA918AD7FA3E05D3" box="[1345,1449,1669,1695]" captionStart="Figs 19" captionStartId="30.[132,175,1353,1375]" captionTargetBox="[135,1457,228,1289]" captionTargetId="figure-7@30.[132,1457,223,1309]" captionTargetPageId="30" captionText="Figs 19al. Spathidium wolfi from life (ah) and after protargol impregnation (il). a right side view of a representative specimen, length 140 µm. The arrowhead marks the anterior contractile vacuole; b frontal view of oral bulge; c oral bulge extrusomes, length 10 µm; d developing extrusomes in the cytoplasm; e slender shape variant, showing the two contractile vacuoles (arrowheads); f development of the “Ringgranula” (cp. Fig. 19a); g, h surface view and optical section, showing the cortical granulation; i, j ventral and dorsal view of anterior body region of a paratype specimen, showing the isostichad dorsal brush and the cuneate oral bulge; k, l right and left side view of anterior body region of the holotype specimen (cp. Figs 20a, b). B(13) dorsal brush rows, BA oral basket, BU oral bulge, CK circumoral kinety, E extrusomes, F oral bulge fibres, G cortical granules, L lipid droplet, MA macronucleus, RG “Ringgranula”. Scale bars: 15 µm (il) and 50 µm (a)." figureDoi="http://doi.org/10.5281/zenodo.10371178" httpUri="https://zenodo.org/record/10371178/files/figure.png" pageId="30" pageNumber="189">Fig. 19d</figureCitation>
); oral bulge extrusomes rod-shaped to indistinctly acicular and slightly curved, about 10 × 0.5 µm in size (
<figureCitation id="6FC02A18FFDCFFBFFA538A98FCED044B" captionStart="Figs 19" captionStartId="30.[132,175,1353,1375]" captionTargetBox="[135,1457,228,1289]" captionTargetId="figure-7@30.[132,1457,223,1309]" captionTargetPageId="30" captionText="Figs 19al. Spathidium wolfi from life (ah) and after protargol impregnation (il). a right side view of a representative specimen, length 140 µm. The arrowhead marks the anterior contractile vacuole; b frontal view of oral bulge; c oral bulge extrusomes, length 10 µm; d developing extrusomes in the cytoplasm; e slender shape variant, showing the two contractile vacuoles (arrowheads); f development of the “Ringgranula” (cp. Fig. 19a); g, h surface view and optical section, showing the cortical granulation; i, j ventral and dorsal view of anterior body region of a paratype specimen, showing the isostichad dorsal brush and the cuneate oral bulge; k, l right and left side view of anterior body region of the holotype specimen (cp. Figs 20a, b). B(13) dorsal brush rows, BA oral basket, BU oral bulge, CK circumoral kinety, E extrusomes, F oral bulge fibres, G cortical granules, L lipid droplet, MA macronucleus, RG “Ringgranula”. Scale bars: 15 µm (il) and 50 µm (a)." figureDoi="http://doi.org/10.5281/zenodo.10371178" httpUri="https://zenodo.org/record/10371178/files/figure.png" pageId="30" pageNumber="189">Figs 19ac</figureCitation>
); do not impregnate with the protargol method used. Cortex flexible, jelly-like, and about 1 µm thick, contains about six rows of colourless, very narrowly spaced granules between each two kineties; individual granules about 0.2 µm across, frequently impregnate with protargol obscuring ciliary pattern (
<figureCitation id="6FC02A18FFE2FF81FDA68897FC910793" box="[630,774,1221,1247]" captionStart="Figs 19" captionStartId="30.[132,175,1353,1375]" captionTargetBox="[135,1457,228,1289]" captionTargetId="figure-7@30.[132,1457,223,1309]" captionTargetPageId="30" captionText="Figs 19al. Spathidium wolfi from life (ah) and after protargol impregnation (il). a right side view of a representative specimen, length 140 µm. The arrowhead marks the anterior contractile vacuole; b frontal view of oral bulge; c oral bulge extrusomes, length 10 µm; d developing extrusomes in the cytoplasm; e slender shape variant, showing the two contractile vacuoles (arrowheads); f development of the “Ringgranula” (cp. Fig. 19a); g, h surface view and optical section, showing the cortical granulation; i, j ventral and dorsal view of anterior body region of a paratype specimen, showing the isostichad dorsal brush and the cuneate oral bulge; k, l right and left side view of anterior body region of the holotype specimen (cp. Figs 20a, b). B(13) dorsal brush rows, BA oral basket, BU oral bulge, CK circumoral kinety, E extrusomes, F oral bulge fibres, G cortical granules, L lipid droplet, MA macronucleus, RG “Ringgranula”. Scale bars: 15 µm (il) and 50 µm (a)." figureDoi="http://doi.org/10.5281/zenodo.10371178" httpUri="https://zenodo.org/record/10371178/files/figure.png" pageId="32" pageNumber="191">
Figs
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, h
</figureCitation>
,
<figureCitation id="6FC02A18FFE2FF81FF5488BAFF22064E" box="[132,181,1256,1282]" captionStart="Figs 22" captionStartId="33.[125,168,1750,1772]" captionTargetBox="[125,1450,228,1713]" captionTargetId="figure-8@33.[125,1450,223,1725]" captionTargetPageId="33" captionText="Figs 22ak. Spathidium wolfi after protargol impregnation. a a slender specimen; b overview of a specimen inflated by the prepara- tion; c, d overviews showing the moniliform macronucleus; eg ventral views, showing the obovate (or broadly cuneate) circumoral kinety; h ventrolateral view, showing kineties and cortical granules; ik anterior body portion at three focal planes. (i) Right side surface view, showing fibres in oral bulge and somatic cortex (arrowheads). (j) When focused slightly deeper, the nematodesmata become visible. (k) When focused to the left side, the dorsal brush and the Spathidium ciliary pattern become recognizable. B(13) dorsal brush (rows), BU oral bulge, CK circumoral kinety, E extrusomes, EP excretory pores, F fibres, G cortical granules, K kineties, MA mac- ronucleus, MI micronuclei, N nematodesmata. Scale bars: 10 µm (ek) and 50 µm (ad)." figureDoi="http://doi.org/10.5281/zenodo.10371186" httpUri="https://zenodo.org/record/10371186/files/figure.png" pageId="32" pageNumber="191">22h</figureCitation>
). Cytoplasm usually dark postorally due to many highly refractive lipid droplets up to 2 µm across and/ or circular structures (“Ringgranula”) 1.52 µm across. Ringgranula develop from globular precursors and do not dissolve when freed from cell; do not impregnate or dissolve with the protargol method used (
<figureCitation id="6FC02A18FFE2FF81FDA789C7FD6A06E3" box="[631,765,1429,1455]" captionStart="Figs 19" captionStartId="30.[132,175,1353,1375]" captionTargetBox="[135,1457,228,1289]" captionTargetId="figure-7@30.[132,1457,223,1309]" captionTargetPageId="30" captionText="Figs 19al. Spathidium wolfi from life (ah) and after protargol impregnation (il). a right side view of a representative specimen, length 140 µm. The arrowhead marks the anterior contractile vacuole; b frontal view of oral bulge; c oral bulge extrusomes, length 10 µm; d developing extrusomes in the cytoplasm; e slender shape variant, showing the two contractile vacuoles (arrowheads); f development of the “Ringgranula” (cp. Fig. 19a); g, h surface view and optical section, showing the cortical granulation; i, j ventral and dorsal view of anterior body region of a paratype specimen, showing the isostichad dorsal brush and the cuneate oral bulge; k, l right and left side view of anterior body region of the holotype specimen (cp. Figs 20a, b). B(13) dorsal brush rows, BA oral basket, BU oral bulge, CK circumoral kinety, E extrusomes, F oral bulge fibres, G cortical granules, L lipid droplet, MA macronucleus, RG “Ringgranula”. Scale bars: 15 µm (il) and 50 µm (a)." figureDoi="http://doi.org/10.5281/zenodo.10371178" httpUri="https://zenodo.org/record/10371178/files/figure.png" pageId="32" pageNumber="191">Figs 19a, f</figureCitation>
). Food not known. Movement without peculiarities.
</paragraph>
<caption id="A3846615FFDCFFBFFF54891BFE62056F" ID-DOI="http://doi.org/10.5281/zenodo.10371178" ID-Zenodo-Dep="10371178" httpUri="https://zenodo.org/record/10371178/files/figure.png" pageId="30" pageNumber="189" startId="30.[132,175,1353,1375]" targetBox="[135,1457,228,1289]" targetPageId="30" targetType="figure">
<paragraph id="F744369DFFDCFFBFFF54891BFE62056F" blockId="30.[131,1464,1353,1571]" pageId="30" pageNumber="189">
<emphasis id="C58FEA8FFFDCFFBFFF54891BFF660612" bold="true" box="[132,241,1353,1375]" pageId="30" pageNumber="189">Figs 19al.</emphasis>
<taxonomicName id="30FB4D1EFFDCFFBFFF27891BFE0D0612" authorityName="Foissner &amp; Wolf &amp; Kumar &amp; Quintela-Alonso" authorityYear="2014" box="[247,410,1353,1374]" class="Gymnostomatea" family="Spathidiidae" genus="Spathidium" kingdom="Chromista" order="Spathidiida" pageId="30" pageNumber="189" phylum="Ciliophora" rank="species" species="wolfi">
<emphasis id="C58FEA8FFFDCFFBFFF27891BFE0D0612" box="[247,410,1353,1374]" italics="true" pageId="30" pageNumber="189">Spathidium wolfi</emphasis>
</taxonomicName>
from life (ah) and after protargol impregnation (il).
<emphasis id="C58FEA8FFFDCFFBFFC758918FC260613" bold="true" box="[933,945,1354,1375]" pageId="30" pageNumber="189">a</emphasis>
right side view of a representative specimen, length 140 µm. The arrowhead marks the anterior contractile vacuole;
<emphasis id="C58FEA8FFFDCFFBFFD248934FC960637" bold="true" box="[756,769,1382,1403]" pageId="30" pageNumber="189">b</emphasis>
frontal view of oral bulge;
<emphasis id="C58FEA8FFFDCFFBFFBF48934FBB80637" bold="true" box="[1060,1071,1382,1403]" pageId="30" pageNumber="189">c</emphasis>
oral bulge extrusomes, length 10 µm;
<emphasis id="C58FEA8FFFDCFFBFFF5489D0FF0606DB" bold="true" box="[132,145,1410,1431]" pageId="30" pageNumber="189">d</emphasis>
developing extrusomes in the cytoplasm;
<emphasis id="C58FEA8FFFDCFFBFFDE489D0FDA806DB" bold="true" box="[564,575,1410,1431]" pageId="30" pageNumber="189">e</emphasis>
slender shape variant, showing the two contractile vacuoles (arrowheads);
<emphasis id="C58FEA8FFFDCFFBFFACC89D3FAB306DB" bold="true" box="[1308,1316,1409,1431]" pageId="30" pageNumber="189">f</emphasis>
development of the “Ringgranula” (cp. Fig. 19a);
<emphasis id="C58FEA8FFFDCFFBFFE3289CCFE7906FF" bold="true" box="[482,494,1438,1459]" pageId="30" pageNumber="189">g</emphasis>
,
<emphasis id="C58FEA8FFFDCFFBFFE2A89CCFD9006FF" bold="true" box="[506,519,1438,1459]" pageId="30" pageNumber="189">h</emphasis>
surface view and optical section, showing the cortical granulation;
<emphasis id="C58FEA8FFFDCFFBFFB7389CFFB3D06FF" bold="true" box="[1187,1194,1437,1459]" pageId="30" pageNumber="189">i</emphasis>
,
<emphasis id="C58FEA8FFFDCFFBFFB6689CFFB2906FF" bold="true" box="[1206,1214,1437,1459]" pageId="30" pageNumber="189">j</emphasis>
ventral and dorsal view of anterior body region of a paratype specimen, showing the isostichad dorsal brush and the cuneate oral bulge;
<emphasis id="C58FEA8FFFDCFFBFFB7889E8FB220683" bold="true" box="[1192,1205,1466,1487]" pageId="30" pageNumber="189">k</emphasis>
,
<emphasis id="C58FEA8FFFDCFFBFFB1089E8FB500683" bold="true" box="[1216,1223,1466,1487]" pageId="30" pageNumber="189">l</emphasis>
right and left side view of anterior body region of the holotype specimen (cp. Figs 20a, b). B(13) dorsal brush rows, BA oral basket, BU oral bulge, CK circumoral kinety, E extrusomes, F oral bulge fibres, G cortical granules, L lipid droplet, MA macronucleus, RG “Ringgranula”. Scale bars: 15 µm (il) and 50 µm (a).
</paragraph>
</caption>
<caption id="A3846615FFDDFFBEFFAD8A3AFC32046A" ID-DOI="http://doi.org/10.5281/zenodo.10371180" ID-Zenodo-Dep="10371180" httpUri="https://zenodo.org/record/10371180/files/figure.png" pageId="31" pageNumber="190" startId="31.[125,168,1640,1662]" targetBox="[146,1430,226,1570]" targetPageId="31" targetType="figure">
<paragraph id="F744369DFFDDFFBEFFAD8A3AFC32046A" blockId="31.[124,1456,1640,1831]" pageId="31" pageNumber="190">
<emphasis id="C58FEA8FFFDDFFBEFFAD8A3AFF7C0532" bold="true" box="[125,235,1640,1662]" pageId="31" pageNumber="190">Figs 20af.</emphasis>
<taxonomicName id="30FB4D1EFFDDFFBEFF208A3BFE050532" authorityName="Foissner &amp; Wolf &amp; Kumar &amp; Quintela-Alonso" authorityYear="2014" box="[240,402,1641,1662]" class="Gymnostomatea" family="Spathidiidae" genus="Spathidium" kingdom="Chromista" order="Spathidiida" pageId="31" pageNumber="190" phylum="Ciliophora" rank="species" species="wolfi">
<emphasis id="C58FEA8FFFDDFFBEFF208A3BFE050532" box="[240,402,1641,1662]" italics="true" pageId="31" pageNumber="190">Spathidium wolfi</emphasis>
</taxonomicName>
from life (c) and after protargol impregnation (a, b, df).
<emphasis id="C58FEA8FFFDDFFBEFC648A3BFC570532" bold="true" box="[948,960,1641,1662]" pageId="31" pageNumber="190">a</emphasis>
,
<emphasis id="C58FEA8FFFDDFFBEFC1C8A3BFC4E0532" bold="true" box="[972,985,1641,1662]" pageId="31" pageNumber="190">b</emphasis>
ciliary pattern of right and left side and nuclear apparatus of holotype specimen; for oral details, see Figs 19k, l. The arrowhead marks the excretory pores of the anterior contractile vacuole, i.e., the main character of this species;
<emphasis id="C58FEA8FFFDDFFBEFDC98AF3FDB305FA" bold="true" box="[537,548,1697,1718]" pageId="31" pageNumber="190">c</emphasis>
supposed structure of dorsal brush row 3;
<emphasis id="C58FEA8FFFDDFFBEFC1C8AF3FC4E05FA" bold="true" box="[972,985,1697,1718]" pageId="31" pageNumber="190">d</emphasis>
ventral view of a paratype specimen with open circumoral kinety (arrow) and thus resembling
<taxonomicName id="30FB4D1EFFDDFFBEFD998AEFFD4B059E" authorityName="Foissner, Agatha &amp; Berger" authorityYear="2002" box="[585,732,1725,1746]" class="Gymnostomatea" family="Apertospathulidae" genus="Apertospathula" kingdom="Chromista" order="Spathidiida" pageId="31" pageNumber="190" phylum="Ciliophora" rank="genus">
<emphasis id="C58FEA8FFFDDFFBEFD998AEFFD4B059E" box="[585,732,1725,1746]" italics="true" pageId="31" pageNumber="190">Apertospathula</emphasis>
</taxonomicName>
;
<emphasis id="C58FEA8FFFDDFFBEFD3A8AEFFD62059E" bold="true" box="[746,757,1725,1746]" pageId="31" pageNumber="190">e</emphasis>
dorsolateral view of a paratype specimen, showing the ciliary pattern and the excretory pores of the anterior and posterior contractile vacuole;
<emphasis id="C58FEA8FFFDDFFBEFCE38A8AFCAC05A2" bold="true" box="[819,827,1752,1774]" pageId="31" pageNumber="190">f</emphasis>
ventrolateral view of oral body region, showing the
<taxonomicName id="30FB4D1EFFDDFFBEFA918A8BFA3905A2" box="[1345,1454,1753,1774]" class="Gymnostomatea" family="Spathidiidae" genus="Spathidium" kingdom="Chromista" order="Spathidiida" pageId="31" pageNumber="190" phylum="Ciliophora" rank="genus">
<emphasis id="C58FEA8FFFDDFFBEFA918A8BFA3905A2" box="[1345,1454,1753,1774]" italics="true" pageId="31" pageNumber="190">Spathidium</emphasis>
</taxonomicName>
ciliary pattern. B(13) dorsal brush (rows), BU oral bulge, CK circumoral kinety, CV contractile vacuole, EP excretory pores, MA macronucleus, MI micronuclei. Scale bars: 10 µm (d, f) and 50 µm (a, b, e).
</paragraph>
</caption>
<caption id="A3846615FFE2FF81FF548FA7FD35070F" ID-DOI="http://doi.org/10.5281/zenodo.10371184" ID-Zenodo-Dep="10371184" httpUri="https://zenodo.org/record/10371184/files/figure.png" pageId="32" pageNumber="191" startId="32.[132,175,1013,1035]" targetBox="[132,1457,223,981]" targetPageId="32" targetType="figure">
<paragraph id="F744369DFFE2FF81FF548FA7FD35070F" blockId="32.[132,1463,1013,1091]" pageId="32" pageNumber="191">
<emphasis id="C58FEA8FFFE2FF81FF548FA7FF6E0746" bold="true" box="[132,249,1013,1035]" pageId="32" pageNumber="191">Figs 21ac.</emphasis>
<taxonomicName id="30FB4D1EFFE2FF81FED28FA7FE3F0746" authorityName="Foissner &amp; Wolf &amp; Kumar &amp; Quintela-Alonso" authorityYear="2014" box="[258,424,1013,1034]" class="Gymnostomatea" family="Spathidiidae" genus="Spathidium" kingdom="Chromista" order="Spathidiida" pageId="32" pageNumber="191" phylum="Ciliophora" rank="species" species="wolfi">
<emphasis id="C58FEA8FFFE2FF81FED28FA7FE3F0746" box="[258,424,1013,1034]" italics="true" pageId="32" pageNumber="191">Spathidium wolfi</emphasis>
</taxonomicName>
, dorsal views of anterior body region after protargol impregnation, showing the isostichad dorsal brush (c, arrows) and the excretory pores (arrowheads) of the anterior contractile vacuole. B(13) dorsal brush rows, BU oral bulge, CK circumoral kinety, MA macronucleus. Scale bars: 20 µm.
</paragraph>
</caption>
<paragraph id="F744369DFFE2FF81FF7A8988FB7F064E" blockId="32.[131,781,1221,1941]" lastBlockId="32.[816,1464,1221,1941]" pageId="32" pageNumber="191">
Cilia about 10 µm long
<emphasis id="C58FEA8FFFE2FF81FE128989FD8606B8" box="[450,529,1499,1524]" italics="true" pageId="32" pageNumber="191">in vivo</emphasis>
, arranged in an average of 15 equidistant, usually bipolar, ordinarily spaced and ciliated rows abutting on circumoral kinety in fairly indistinct
<taxonomicName id="30FB4D1EFFE2FF81FF2D8A10FE140510" box="[253,387,1602,1628]" class="Gymnostomatea" family="Spathidiidae" genus="Spathidium" kingdom="Chromista" order="Spathidiida" pageId="32" pageNumber="191" phylum="Ciliophora" rank="genus">
<emphasis id="C58FEA8FFFE2FF81FF2D8A10FE140510" box="[253,387,1602,1628]" italics="true" pageId="32" pageNumber="191">Spathidium</emphasis>
</taxonomicName>
pattern because ventral half kineties lack polymerized kinetids anteriorly and do not abut on circumoral kinety (
<figureCitation id="6FC02A18FFE2FF81FE128ADAFDD605EE" box="[450,577,1672,1698]" captionStart="Figs 19" captionStartId="30.[132,175,1353,1375]" captionTargetBox="[135,1457,228,1289]" captionTargetId="figure-7@30.[132,1457,223,1309]" captionTargetPageId="30" captionText="Figs 19al. Spathidium wolfi from life (ah) and after protargol impregnation (il). a right side view of a representative specimen, length 140 µm. The arrowhead marks the anterior contractile vacuole; b frontal view of oral bulge; c oral bulge extrusomes, length 10 µm; d developing extrusomes in the cytoplasm; e slender shape variant, showing the two contractile vacuoles (arrowheads); f development of the “Ringgranula” (cp. Fig. 19a); g, h surface view and optical section, showing the cortical granulation; i, j ventral and dorsal view of anterior body region of a paratype specimen, showing the isostichad dorsal brush and the cuneate oral bulge; k, l right and left side view of anterior body region of the holotype specimen (cp. Figs 20a, b). B(13) dorsal brush rows, BA oral basket, BU oral bulge, CK circumoral kinety, E extrusomes, F oral bulge fibres, G cortical granules, L lipid droplet, MA macronucleus, RG “Ringgranula”. Scale bars: 15 µm (il) and 50 µm (a)." figureDoi="http://doi.org/10.5281/zenodo.10371178" httpUri="https://zenodo.org/record/10371178/files/figure.png" pageId="32" pageNumber="191">Figs 19k, l</figureCitation>
,
<figureCitation id="6FC02A18FFE2FF81FD9E8ADAFD5905EE" box="[590,718,1672,1698]" captionStart="Figs 20" captionStartId="31.[125,168,1640,1662]" captionTargetBox="[146,1430,226,1570]" captionTargetId="figure-8@31.[125,1450,223,1587]" captionTargetPageId="31" captionText="Figs 20af. Spathidium wolfi from life (c) and after protargol impregnation (a, b, df). a, b ciliary pattern of right and left side and nuclear apparatus of holotype specimen; for oral details, see Figs 19k, l. The arrowhead marks the excretory pores of the anterior contractile vacu- ole, i.e., the main character of this species; c supposed structure of dorsal brush row 3; d ventral view of a paratype specimen with open circumoral kinety (arrow) and thus resembling Apertospathula; e dorsolateral view of a paratype specimen, showing the ciliary pattern and the excretory pores of the anterior and posterior contractile vacuole; f ventrolateral view of oral body region, showing the Spathidium ciliary pattern. B(13) dorsal brush (rows), BU oral bulge, CK circumoral kinety, CV contractile vacuole, EP excretory pores, MA macronucleus, MI micronuclei. Scale bars: 10 µm (d, f) and 50 µm (a, b, e)." figureDoi="http://doi.org/10.5281/zenodo.10371180" httpUri="https://zenodo.org/record/10371180/files/figure.png" pageId="32" pageNumber="191">20a, b, df</figureCitation>
,
<figureCitation id="6FC02A18FFE2FF81FD098ADAFC9F05EE" box="[729,776,1672,1698]" captionStart="Figs 22" captionStartId="33.[125,168,1750,1772]" captionTargetBox="[125,1450,228,1713]" captionTargetId="figure-8@33.[125,1450,223,1725]" captionTargetPageId="33" captionText="Figs 22ak. Spathidium wolfi after protargol impregnation. a a slender specimen; b overview of a specimen inflated by the prepara- tion; c, d overviews showing the moniliform macronucleus; eg ventral views, showing the obovate (or broadly cuneate) circumoral kinety; h ventrolateral view, showing kineties and cortical granules; ik anterior body portion at three focal planes. (i) Right side surface view, showing fibres in oral bulge and somatic cortex (arrowheads). (j) When focused slightly deeper, the nematodesmata become visible. (k) When focused to the left side, the dorsal brush and the Spathidium ciliary pattern become recognizable. B(13) dorsal brush (rows), BU oral bulge, CK circumoral kinety, E extrusomes, EP excretory pores, F fibres, G cortical granules, K kineties, MA mac- ronucleus, MI micronuclei, N nematodesmata. Scale bars: 10 µm (ek) and 50 µm (ad)." figureDoi="http://doi.org/10.5281/zenodo.10371186" httpUri="https://zenodo.org/record/10371186/files/figure.png" pageId="32" pageNumber="191">22k</figureCitation>
;
<tableCitation id="BA790326FFE2FF81FF548AF8FF750589" box="[132,226,1706,1733]" captionStart="Table 7" captionStartId="29.[125,181,223,244]" captionText="Table 7. Morphometric data on Spathidium wolfi." pageId="32" pageNumber="191">Table 7</tableCitation>
). Dorsal brush of ordinary structure and distinctness, occupies about 19% of body length, bristle details difficult to recognize
<emphasis id="C58FEA8FFFE2FF81FE1E8AA3FD8B0446" box="[462,540,1777,1802]" italics="true" pageId="32" pageNumber="191">in vivo</emphasis>
due to the highly refractive cytoplasmic inclusions. Brush rows isostichad, each with distinct anterior tail and likely structured as shown in
<figureCitation id="6FC02A18FFE2FF81FF288B0AFECD043E" box="[248,346,1880,1906]" captionStart="Figs 20" captionStartId="31.[125,168,1640,1662]" captionTargetBox="[146,1430,226,1570]" captionTargetId="figure-8@31.[125,1450,223,1587]" captionTargetPageId="31" captionText="Figs 20af. Spathidium wolfi from life (c) and after protargol impregnation (a, b, df). a, b ciliary pattern of right and left side and nuclear apparatus of holotype specimen; for oral details, see Figs 19k, l. The arrowhead marks the excretory pores of the anterior contractile vacu- ole, i.e., the main character of this species; c supposed structure of dorsal brush row 3; d ventral view of a paratype specimen with open circumoral kinety (arrow) and thus resembling Apertospathula; e dorsolateral view of a paratype specimen, showing the ciliary pattern and the excretory pores of the anterior and posterior contractile vacuole; f ventrolateral view of oral body region, showing the Spathidium ciliary pattern. B(13) dorsal brush (rows), BU oral bulge, CK circumoral kinety, CV contractile vacuole, EP excretory pores, MA macronucleus, MI micronuclei. Scale bars: 10 µm (d, f) and 50 µm (a, b, e)." figureDoi="http://doi.org/10.5281/zenodo.10371180" httpUri="https://zenodo.org/record/10371180/files/figure.png" pageId="32" pageNumber="191">Fig. 20c</figureCitation>
, that is, with some 56 µm long bristles in posterior half; row 1 composed of an average of 16 dikinetids, row 2 of 18, and row 3 of 14 (
<figureCitation id="6FC02A18FFE2FF81FA988897FCCD064E" captionStart="Figs 19" captionStartId="30.[132,175,1353,1375]" captionTargetBox="[135,1457,228,1289]" captionTargetId="figure-7@30.[132,1457,223,1309]" captionTargetPageId="30" captionText="Figs 19al. Spathidium wolfi from life (ah) and after protargol impregnation (il). a right side view of a representative specimen, length 140 µm. The arrowhead marks the anterior contractile vacuole; b frontal view of oral bulge; c oral bulge extrusomes, length 10 µm; d developing extrusomes in the cytoplasm; e slender shape variant, showing the two contractile vacuoles (arrowheads); f development of the “Ringgranula” (cp. Fig. 19a); g, h surface view and optical section, showing the cortical granulation; i, j ventral and dorsal view of anterior body region of a paratype specimen, showing the isostichad dorsal brush and the cuneate oral bulge; k, l right and left side view of anterior body region of the holotype specimen (cp. Figs 20a, b). B(13) dorsal brush rows, BA oral basket, BU oral bulge, CK circumoral kinety, E extrusomes, F oral bulge fibres, G cortical granules, L lipid droplet, MA macronucleus, RG “Ringgranula”. Scale bars: 15 µm (il) and 50 µm (a)." figureDoi="http://doi.org/10.5281/zenodo.10371178" httpUri="https://zenodo.org/record/10371178/files/figure.png" pageId="32" pageNumber="191">Figs 19a, ik</figureCitation>
,
<figureCitation id="6FC02A18FFE2FF81FCB688BAFC50064E" box="[870,967,1256,1282]" captionStart="Figs 20" captionStartId="31.[125,168,1640,1662]" captionTargetBox="[146,1430,226,1570]" captionTargetId="figure-8@31.[125,1450,223,1587]" captionTargetPageId="31" captionText="Figs 20af. Spathidium wolfi from life (c) and after protargol impregnation (a, b, df). a, b ciliary pattern of right and left side and nuclear apparatus of holotype specimen; for oral details, see Figs 19k, l. The arrowhead marks the excretory pores of the anterior contractile vacu- ole, i.e., the main character of this species; c supposed structure of dorsal brush row 3; d ventral view of a paratype specimen with open circumoral kinety (arrow) and thus resembling Apertospathula; e dorsolateral view of a paratype specimen, showing the ciliary pattern and the excretory pores of the anterior and posterior contractile vacuole; f ventrolateral view of oral body region, showing the Spathidium ciliary pattern. B(13) dorsal brush (rows), BU oral bulge, CK circumoral kinety, CV contractile vacuole, EP excretory pores, MA macronucleus, MI micronuclei. Scale bars: 10 µm (d, f) and 50 µm (a, b, e)." figureDoi="http://doi.org/10.5281/zenodo.10371180" httpUri="https://zenodo.org/record/10371180/files/figure.png" pageId="32" pageNumber="191">20a, e, f</figureCitation>
,
<figureCitation id="6FC02A18FFE2FF81FC0388BAFB8B064E" box="[979,1052,1256,1282]" captionStart="Figs 21" captionStartId="32.[132,175,1013,1035]" captionTargetBox="[132,1457,223,981]" captionTargetId="figure-7@32.[132,1457,223,990]" captionTargetPageId="32" captionText="Figs 21ac. Spathidium wolfi, dorsal views of anterior body region after protargol impregnation, showing the isostichad dorsal brush (c, arrows) and the excretory pores (arrowheads) of the anterior contractile vacuole. B(13) dorsal brush rows, BU oral bulge, CK cir- cumoral kinety, MA macronucleus. Scale bars: 20 µm." figureDoi="http://doi.org/10.5281/zenodo.10371184" httpUri="https://zenodo.org/record/10371184/files/figure.png" pageId="32" pageNumber="191">21ac</figureCitation>
,
<figureCitation id="6FC02A18FFE2FF81FBF888BAFBE2064E" box="[1064,1141,1256,1282]" captionStart="Figs 22" captionStartId="33.[125,168,1750,1772]" captionTargetBox="[125,1450,228,1713]" captionTargetId="figure-8@33.[125,1450,223,1725]" captionTargetPageId="33" captionText="Figs 22ak. Spathidium wolfi after protargol impregnation. a a slender specimen; b overview of a specimen inflated by the prepara- tion; c, d overviews showing the moniliform macronucleus; eg ventral views, showing the obovate (or broadly cuneate) circumoral kinety; h ventrolateral view, showing kineties and cortical granules; ik anterior body portion at three focal planes. (i) Right side surface view, showing fibres in oral bulge and somatic cortex (arrowheads). (j) When focused slightly deeper, the nematodesmata become visible. (k) When focused to the left side, the dorsal brush and the Spathidium ciliary pattern become recognizable. B(13) dorsal brush (rows), BU oral bulge, CK circumoral kinety, E extrusomes, EP excretory pores, F fibres, G cortical granules, K kineties, MA mac- ronucleus, MI micronuclei, N nematodesmata. Scale bars: 10 µm (ek) and 50 µm (ad)." figureDoi="http://doi.org/10.5281/zenodo.10371186" httpUri="https://zenodo.org/record/10371186/files/figure.png" pageId="32" pageNumber="191">22b, k</figureCitation>
;
<tableCitation id="BA790326FFE2FF81FB5088BAFB4E064E" box="[1152,1241,1256,1282]" captionStart="Table 7" captionStartId="29.[125,181,223,244]" captionText="Table 7. Morphometric data on Spathidium wolfi." pageId="32" pageNumber="191">Table 7</tableCitation>
).
</paragraph>
<paragraph id="F744369DFFE2FF81FC868958FA0804D9" blockId="32.[816,1464,1221,1941]" pageId="32" pageNumber="191">
Oral bulge occupies anterior body end slanted by 45° to 60°, on average half as long as widest trunk region in inflated protargol-impregnated specimens (see above), while about as long as trunk width
<emphasis id="C58FEA8FFFE2FF81FAEB8921FA1B06C0" box="[1339,1420,1395,1420]" italics="true" pageId="32" pageNumber="191">in vivo</emphasis>
; of ordinary distinctness in lateral view, that is, about 4 µm high and with flat to slightly convex surface, while con- spicuously obovate to cuneate in frontal view (
<figureCitation id="6FC02A18FFE2FF81FA9A8988FA2506B8" box="[1354,1458,1498,1524]" captionStart="Figs 19" captionStartId="30.[132,175,1353,1375]" captionTargetBox="[135,1457,228,1289]" captionTargetId="figure-7@30.[132,1457,223,1309]" captionTargetPageId="30" captionText="Figs 19al. Spathidium wolfi from life (ah) and after protargol impregnation (il). a right side view of a representative specimen, length 140 µm. The arrowhead marks the anterior contractile vacuole; b frontal view of oral bulge; c oral bulge extrusomes, length 10 µm; d developing extrusomes in the cytoplasm; e slender shape variant, showing the two contractile vacuoles (arrowheads); f development of the “Ringgranula” (cp. Fig. 19a); g, h surface view and optical section, showing the cortical granulation; i, j ventral and dorsal view of anterior body region of a paratype specimen, showing the isostichad dorsal brush and the cuneate oral bulge; k, l right and left side view of anterior body region of the holotype specimen (cp. Figs 20a, b). B(13) dorsal brush rows, BA oral basket, BU oral bulge, CK circumoral kinety, E extrusomes, F oral bulge fibres, G cortical granules, L lipid droplet, MA macronucleus, RG “Ringgranula”. Scale bars: 15 µm (il) and 50 µm (a)." figureDoi="http://doi.org/10.5281/zenodo.10371178" httpUri="https://zenodo.org/record/10371178/files/figure.png" pageId="32" pageNumber="191">Figs 19b</figureCitation>
,
<figureCitation id="6FC02A18FFE2FF81FCE089AFFCC9055B" box="[816,862,1533,1559]" captionStart="Figs 20" captionStartId="31.[125,168,1640,1662]" captionTargetBox="[146,1430,226,1570]" captionTargetId="figure-8@31.[125,1450,223,1587]" captionTargetPageId="31" captionText="Figs 20af. Spathidium wolfi from life (c) and after protargol impregnation (a, b, df). a, b ciliary pattern of right and left side and nuclear apparatus of holotype specimen; for oral details, see Figs 19k, l. The arrowhead marks the excretory pores of the anterior contractile vacu- ole, i.e., the main character of this species; c supposed structure of dorsal brush row 3; d ventral view of a paratype specimen with open circumoral kinety (arrow) and thus resembling Apertospathula; e dorsolateral view of a paratype specimen, showing the ciliary pattern and the excretory pores of the anterior and posterior contractile vacuole; f ventrolateral view of oral body region, showing the Spathidium ciliary pattern. B(13) dorsal brush (rows), BU oral bulge, CK circumoral kinety, CV contractile vacuole, EP excretory pores, MA macronucleus, MI micronuclei. Scale bars: 10 µm (d, f) and 50 µm (a, b, e)." figureDoi="http://doi.org/10.5281/zenodo.10371180" httpUri="https://zenodo.org/record/10371180/files/figure.png" pageId="32" pageNumber="191">20d</figureCitation>
,
<figureCitation id="6FC02A18FFE2FF81FCBA89AFFC22055B" box="[874,949,1533,1559]" captionStart="Figs 22" captionStartId="33.[125,168,1750,1772]" captionTargetBox="[125,1450,228,1713]" captionTargetId="figure-8@33.[125,1450,223,1725]" captionTargetPageId="33" captionText="Figs 22ak. Spathidium wolfi after protargol impregnation. a a slender specimen; b overview of a specimen inflated by the prepara- tion; c, d overviews showing the moniliform macronucleus; eg ventral views, showing the obovate (or broadly cuneate) circumoral kinety; h ventrolateral view, showing kineties and cortical granules; ik anterior body portion at three focal planes. (i) Right side surface view, showing fibres in oral bulge and somatic cortex (arrowheads). (j) When focused slightly deeper, the nematodesmata become visible. (k) When focused to the left side, the dorsal brush and the Spathidium ciliary pattern become recognizable. B(13) dorsal brush (rows), BU oral bulge, CK circumoral kinety, E extrusomes, EP excretory pores, F fibres, G cortical granules, K kineties, MA mac- ronucleus, MI micronuclei, N nematodesmata. Scale bars: 10 µm (ek) and 50 µm (ad)." figureDoi="http://doi.org/10.5281/zenodo.10371186" httpUri="https://zenodo.org/record/10371186/files/figure.png" pageId="32" pageNumber="191">22eg</figureCitation>
), a rare shape in
<taxonomicName id="30FB4D1EFFE2FF81FBAE89AFFA95055B" box="[1150,1282,1533,1559]" class="Gymnostomatea" family="Spathidiidae" genus="Spathidium" kingdom="Chromista" order="Spathidiida" pageId="32" pageNumber="191" phylum="Ciliophora" rank="genus" sensu="stricto">
<emphasis id="C58FEA8FFFE2FF81FBAE89AFFA95055B" box="[1150,1282,1533,1559]" italics="true" pageId="32" pageNumber="191">Spathidium</emphasis>
</taxonomicName>
<taxonomicNameLabel id="DEBC57F4FFE2FF81FAD989ACFADE0554" box="[1289,1353,1534,1560]" pageId="32" pageNumber="191" sensu="stricto">s. str.</taxonomicNameLabel>
; contains rather thick fibres originating from circumoral dikinetids; cytopharyngeal entrance not recognizable. Circumoral kinety of same shape as oral bulge, continuous but occasionally with a minute gap ventrally, similar as in
<taxonomicName id="30FB4D1EFFE2FF81FC9E8AF8FB960588" authorityName="Foissner, Agatha &amp; Berger" authorityYear="2002" box="[846,1025,1706,1732]" class="Gymnostomatea" family="Apertospathulidae" genus="Apertospathula" kingdom="Chromista" order="Spathidiida" pageId="32" pageNumber="191" phylum="Ciliophora" rank="genus">
<emphasis id="C58FEA8FFFE2FF81FC9E8AF8FB960588" box="[846,1025,1706,1732]" italics="true" pageId="32" pageNumber="191">Apertospathula</emphasis>
</taxonomicName>
(
<figureCitation id="6FC02A18FFE2FF81FBC08AF8FBE10588" box="[1040,1142,1706,1732]" captionStart="Figs 20" captionStartId="31.[125,168,1640,1662]" captionTargetBox="[146,1430,226,1570]" captionTargetId="figure-8@31.[125,1450,223,1587]" captionTargetPageId="31" captionText="Figs 20af. Spathidium wolfi from life (c) and after protargol impregnation (a, b, df). a, b ciliary pattern of right and left side and nuclear apparatus of holotype specimen; for oral details, see Figs 19k, l. The arrowhead marks the excretory pores of the anterior contractile vacu- ole, i.e., the main character of this species; c supposed structure of dorsal brush row 3; d ventral view of a paratype specimen with open circumoral kinety (arrow) and thus resembling Apertospathula; e dorsolateral view of a paratype specimen, showing the ciliary pattern and the excretory pores of the anterior and posterior contractile vacuole; f ventrolateral view of oral body region, showing the Spathidium ciliary pattern. B(13) dorsal brush (rows), BU oral bulge, CK circumoral kinety, CV contractile vacuole, EP excretory pores, MA macronucleus, MI micronuclei. Scale bars: 10 µm (d, f) and 50 µm (a, b, e)." figureDoi="http://doi.org/10.5281/zenodo.10371180" httpUri="https://zenodo.org/record/10371180/files/figure.png" pageId="32" pageNumber="191">Fig. 20d</figureCitation>
); composed of about 4080 dikinetids each associated with a cilium, an oral basket rod, and a distinct fibre extending into oral bulge cortex, as described above. Nematodesmata fairly distinct, form small bundles slightly longer than dorsal brush; oral basket thus rather distinct in appropriately impregnated specimens (
<figureCitation id="6FC02A18FFE2FF81FC698B28FBDA04D8" box="[953,1101,1914,1941]" captionStart="Figs 19" captionStartId="30.[132,175,1353,1375]" captionTargetBox="[135,1457,228,1289]" captionTargetId="figure-7@30.[132,1457,223,1309]" captionTargetPageId="30" captionText="Figs 19al. Spathidium wolfi from life (ah) and after protargol impregnation (il). a right side view of a representative specimen, length 140 µm. The arrowhead marks the anterior contractile vacuole; b frontal view of oral bulge; c oral bulge extrusomes, length 10 µm; d developing extrusomes in the cytoplasm; e slender shape variant, showing the two contractile vacuoles (arrowheads); f development of the “Ringgranula” (cp. Fig. 19a); g, h surface view and optical section, showing the cortical granulation; i, j ventral and dorsal view of anterior body region of a paratype specimen, showing the isostichad dorsal brush and the cuneate oral bulge; k, l right and left side view of anterior body region of the holotype specimen (cp. Figs 20a, b). B(13) dorsal brush rows, BA oral basket, BU oral bulge, CK circumoral kinety, E extrusomes, F oral bulge fibres, G cortical granules, L lipid droplet, MA macronucleus, RG “Ringgranula”. Scale bars: 15 µm (il) and 50 µm (a)." figureDoi="http://doi.org/10.5281/zenodo.10371178" httpUri="https://zenodo.org/record/10371178/files/figure.png" pageId="32" pageNumber="191">Figs 19a, il</figureCitation>
,
<figureCitation id="6FC02A18FFE2FF81FB898B29FB4004D8" box="[1113,1239,1914,1941]" captionStart="Figs 20" captionStartId="31.[125,168,1640,1662]" captionTargetBox="[146,1430,226,1570]" captionTargetId="figure-8@31.[125,1450,223,1587]" captionTargetPageId="31" captionText="Figs 20af. Spathidium wolfi from life (c) and after protargol impregnation (a, b, df). a, b ciliary pattern of right and left side and nuclear apparatus of holotype specimen; for oral details, see Figs 19k, l. The arrowhead marks the excretory pores of the anterior contractile vacu- ole, i.e., the main character of this species; c supposed structure of dorsal brush row 3; d ventral view of a paratype specimen with open circumoral kinety (arrow) and thus resembling Apertospathula; e dorsolateral view of a paratype specimen, showing the ciliary pattern and the excretory pores of the anterior and posterior contractile vacuole; f ventrolateral view of oral body region, showing the Spathidium ciliary pattern. B(13) dorsal brush (rows), BU oral bulge, CK circumoral kinety, CV contractile vacuole, EP excretory pores, MA macronucleus, MI micronuclei. Scale bars: 10 µm (d, f) and 50 µm (a, b, e)." figureDoi="http://doi.org/10.5281/zenodo.10371180" httpUri="https://zenodo.org/record/10371180/files/figure.png" pageId="32" pageNumber="191">20a, b, d, f</figureCitation>
,
<figureCitation id="6FC02A18FFE2FF81FB338B28FAB904D8" box="[1251,1326,1914,1940]" captionStart="Figs 22" captionStartId="33.[125,168,1750,1772]" captionTargetBox="[125,1450,228,1713]" captionTargetId="figure-8@33.[125,1450,223,1725]" captionTargetPageId="33" captionText="Figs 22ak. Spathidium wolfi after protargol impregnation. a a slender specimen; b overview of a specimen inflated by the prepara- tion; c, d overviews showing the moniliform macronucleus; eg ventral views, showing the obovate (or broadly cuneate) circumoral kinety; h ventrolateral view, showing kineties and cortical granules; ik anterior body portion at three focal planes. (i) Right side surface view, showing fibres in oral bulge and somatic cortex (arrowheads). (j) When focused slightly deeper, the nematodesmata become visible. (k) When focused to the left side, the dorsal brush and the Spathidium ciliary pattern become recognizable. B(13) dorsal brush (rows), BU oral bulge, CK circumoral kinety, E extrusomes, EP excretory pores, F fibres, G cortical granules, K kineties, MA mac- ronucleus, MI micronuclei, N nematodesmata. Scale bars: 10 µm (ek) and 50 µm (ad)." figureDoi="http://doi.org/10.5281/zenodo.10371186" httpUri="https://zenodo.org/record/10371186/files/figure.png" pageId="32" pageNumber="191">22ek</figureCitation>
;
<tableCitation id="BA790326FFE2FF81FAE98B28FA0604D9" box="[1337,1425,1914,1941]" captionStart="Table 7" captionStartId="29.[125,181,223,244]" captionText="Table 7. Morphometric data on Spathidium wolfi." pageId="32" pageNumber="191">Table 7</tableCitation>
).
</paragraph>
<caption id="A3846615FFE3FF80FFAD8A84FC6004D8" ID-DOI="http://doi.org/10.5281/zenodo.10371186" ID-Zenodo-Dep="10371186" httpUri="https://zenodo.org/record/10371186/files/figure.png" pageId="33" pageNumber="192" startId="33.[125,168,1750,1772]" targetBox="[125,1450,228,1713]" targetPageId="33" targetType="figure">
<paragraph id="F744369DFFE3FF80FFAD8A84FC6004D8" blockId="33.[124,1455,1750,1940]" pageId="33" pageNumber="192">
<emphasis id="C58FEA8FFFE3FF80FFAD8A84FF6505A7" bold="true" box="[125,242,1750,1772]" pageId="33" pageNumber="192">Figs 22ak.</emphasis>
<taxonomicName id="30FB4D1EFFE3FF80FF2A8A84FE0A05A7" authorityName="Foissner &amp; Wolf &amp; Kumar &amp; Quintela-Alonso" authorityYear="2014" box="[250,413,1750,1771]" class="Gymnostomatea" family="Spathidiidae" genus="Spathidium" kingdom="Chromista" order="Spathidiida" pageId="33" pageNumber="192" phylum="Ciliophora" rank="species" species="wolfi">
<emphasis id="C58FEA8FFFE3FF80FF2A8A84FE0A05A7" box="[250,413,1750,1771]" italics="true" pageId="33" pageNumber="192">Spathidium wolfi</emphasis>
</taxonomicName>
after protargol impregnation.
<emphasis id="C58FEA8FFFE3FF80FD168A84FD4505A7" bold="true" box="[710,722,1750,1771]" pageId="33" pageNumber="192">a</emphasis>
a slender specimen;
<emphasis id="C58FEA8FFFE3FF80FC638A84FC5705A7" bold="true" box="[947,960,1750,1771]" pageId="33" pageNumber="192">b</emphasis>
overview of a specimen inflated by the preparation;
<emphasis id="C58FEA8FFFE3FF80FF608AA0FF2C044B" bold="true" box="[176,187,1778,1799]" pageId="33" pageNumber="192">c</emphasis>
,
<emphasis id="C58FEA8FFFE3FF80FF188AA0FF42044B" bold="true" box="[200,213,1778,1799]" pageId="33" pageNumber="192">d</emphasis>
overviews showing the moniliform macronucleus;
<emphasis id="C58FEA8FFFE3FF80FD0E8AA0FC96044B" bold="true" box="[734,769,1778,1799]" pageId="33" pageNumber="192">eg</emphasis>
ventral views, showing the obovate (or broadly cuneate) circumoral kinety;
<emphasis id="C58FEA8FFFE3FF80FF158B5CFF45046F" bold="true" box="[197,210,1806,1827]" pageId="33" pageNumber="192">h</emphasis>
ventrolateral view, showing kineties and cortical granules;
<emphasis id="C58FEA8FFFE3FF80FCC88B5CFCAF0468" bold="true" box="[792,824,1806,1828]" pageId="33" pageNumber="192">ik</emphasis>
anterior body portion at three focal planes. (i) Right side surface view, showing fibres in oral bulge and somatic cortex (arrowheads). (j) When focused slightly deeper, the nematodesmata become visible. (k) When focused to the left side, the dorsal brush and the
<taxonomicName id="30FB4D1EFFE3FF80FD678B14FCB30417" box="[695,804,1862,1883]" class="Gymnostomatea" family="Spathidiidae" genus="Spathidium" kingdom="Chromista" order="Spathidiida" pageId="33" pageNumber="192" phylum="Ciliophora" rank="genus">
<emphasis id="C58FEA8FFFE3FF80FD678B14FCB30417" box="[695,804,1862,1883]" italics="true" pageId="33" pageNumber="192">Spathidium</emphasis>
</taxonomicName>
ciliary pattern become recognizable. B(13) dorsal brush (rows), BU oral bulge, CK circumoral kinety, E extrusomes, EP excretory pores, F fibres, G cortical granules, K kineties, MA macronucleus, MI micronuclei, N nematodesmata. Scale bars: 10 µm (ek) and 50 µm (ad).
</paragraph>
</caption>
<paragraph id="F744369DFFE0FF83FF7A8C8DFE98011F" blockId="34.[132,779,223,908]" pageId="34" pageNumber="193">
<emphasis id="C58FEA8FFFE0FF83FF7A8C8DFE7303B5" bold="true" box="[170,484,223,249]" pageId="34" pageNumber="193">Occurrence and ecology:</emphasis>
<materialsCitation id="47933CC0FFE0FF83FE3C8C8DFE9B011F" country="Venezuela" location="Central" municipality="South America. Further" pageId="34" pageNumber="193" specimenCount="2">
As yet found at
<typeStatus id="2840883FFFE0FF83FD7E8C8DFD7603B5" box="[686,737,223,249]" pageId="34" pageNumber="193">type</typeStatus>
locality and in a granitic rockpool (Laja) near to the town of Pto. Ayachuco,
<collectingCountry id="8FEC760DFFE0FF83FE8E8D76FE4D0272" box="[350,474,292,318]" name="Venezuela" pageId="34" pageNumber="193">Venezuela</collectingCountry>
, where a single specimen is contained in the
<typeStatus id="2840883FFFE0FF83FE8E8D15FE06022D" box="[350,401,327,353]" pageId="34" pageNumber="193">type</typeStatus>
slides of
<taxonomicName id="30FB4D1EFFE0FF83FE2B8D15FF0C02CF" authorityName="Foissner &amp; Xu" authorityYear="2007" class="Gymnostomatea" family="Apertospathulidae" genus="Apertospathula" kingdom="Chromista" order="Spathidiida" pageId="34" pageNumber="193" phylum="Ciliophora" rank="species" species="lajacola">
<emphasis id="C58FEA8FFFE0FF83FE2B8D15FF0C02CF" italics="true" pageId="34" pageNumber="193">Apertospathula lajacola</emphasis>
</taxonomicName>
, indicating wide distribution in
<location id="F2246046FFE0FF83FDFD8D3BFD1402CF" LSID="urn:lsid:plazi:treatment:7F52878BFFDEFF83FF548ACBFD2200C0:F2246046FFE0FF83FDFD8D3BFD1402CF" box="[557,643,361,387]" country="Venezuela" municipality="South America. Further" name="Central" pageId="34" pageNumber="193">Central</location>
and
<collectingMunicipality id="1720ACE7FFE0FF83FD168D3BFED802EA" pageId="34" pageNumber="193">South America. Further</collectingMunicipality>
studies are required to determine the extent the species is specific to bromeliad tanks. Num- bers were very low in the fresh sample, but after addition of a squashed wheat grain they multiplied rapidly for some days; however, efforts to establish pure cultures failed
</materialsCitation>
.
</paragraph>
</subSubSection>
<subSubSection id="BFE16516FFE0FF83FF7A8E0EFD2200C0" pageId="34" pageNumber="193" type="discussion">
<paragraph id="F744369DFFE0FF83FF7A8E0EFD2200C0" blockId="34.[132,779,223,908]" pageId="34" pageNumber="193">
<emphasis id="C58FEA8FFFE0FF83FF7A8E0EFEB1013A" bold="true" box="[170,294,604,630]" pageId="34" pageNumber="193">Remarks:</emphasis>
Within the genus,
<taxonomicName id="30FB4D1EFFE0FF83FDDD8E0FFDF1013A" authorityName="Foissner &amp; Wolf &amp; Kumar &amp; Quintela-Alonso" authorityYear="2014" box="[525,614,604,631]" class="Gymnostomatea" family="Spathidiidae" genus="Spathidium" kingdom="Chromista" order="Spathidiida" pageId="34" pageNumber="193" phylum="Ciliophora" rank="species" species="wolfi">
<emphasis id="C58FEA8FFFE0FF83FDDD8E0FFDF1013A" box="[525,614,604,631]" italics="true" pageId="34" pageNumber="193">S. wolfi</emphasis>
</taxonomicName>
and
<taxonomicName id="30FB4D1EFFE0FF83FD748E0FFE0801D5" authority="Foissner (2003)" authorityName="Foissner" authorityYear="2003" class="Gymnostomatea" family="Spathidiidae" genus="Spathidium" kingdom="Chromista" order="Spathidiida" pageId="34" pageNumber="193" phylum="Ciliophora" rank="species" species="faurefremieti">
<emphasis id="C58FEA8FFFE0FF83FD748E0FFF4901D5" italics="true" pageId="34" pageNumber="193">S. faurefremieti</emphasis>
Foissner (2003)
</taxonomicName>
form a distinct subgroup characterized by two contractile vacuoles and a cuneate oral bulge. If further such species are discovered, they should be separated at subgeneric rank, at least. Actually,
<taxonomicName id="30FB4D1EFFE0FF83FF778F58FF6A006F" authorityName="Foissner &amp; Wolf &amp; Kumar &amp; Quintela-Alonso" authorityYear="2014" box="[167,253,777,804]" class="Gymnostomatea" family="Spathidiidae" genus="Spathidium" kingdom="Chromista" order="Spathidiida" pageId="34" pageNumber="193" phylum="Ciliophora" rank="species" species="wolfi">
<emphasis id="C58FEA8FFFE0FF83FF778F58FF6A006F" box="[167,253,777,804]" italics="true" pageId="34" pageNumber="193">S. wolfi</emphasis>
</taxonomicName>
looks like a small
<taxonomicName id="30FB4D1EFFE0FF83FE078F58FD1C0068" authorityName="Foissner" authorityYear="2003" box="[471,651,778,804]" class="Gymnostomatea" family="Spathidiidae" genus="Spathidium" kingdom="Chromista" order="Spathidiida" pageId="34" pageNumber="193" phylum="Ciliophora" rank="species" species="faurefremieti">
<emphasis id="C58FEA8FFFE0FF83FE078F58FD1C0068" box="[471,651,778,804]" italics="true" pageId="34" pageNumber="193">S. faurefremieti</emphasis>
</taxonomicName>
but differs in body size (135 × 25 µm vs. 240 × 17 µm), macronucleus (moniliform vs. a long, tortuous strand), and the total number of brush bristles (about 47 vs. 72).
</paragraph>
</subSubSection>
</treatment>
</document>