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<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.48.30350" ID-GBIF-Dataset="cf87abe2-a0fd-4c1b-a1c3-42106e3f4cb5" ID-PMC="PMC6414474" ID-Pensoft-Pub="1314-4049--1" ID-PubMed="30872942" ModsDocAuthor="" ModsDocDate="2019" ModsDocID="1314-4049--1" ModsDocOrigin="MycoKeys " ModsDocTitle="Species identification of European forest pathogens of the genus Milesina (Pucciniales) using urediniospore morphology and molecular barcoding including M.woodwardiana sp. nov." checkinTime="1553125542693" checkinUser="pensoft" docAuthor="Bubner, Ben, Buchheit, Ramona, Friedrich, Frank, Kummer, Volker &amp; Scholler, Markus" docDate="2019" docId="150FFBF2FD44281B0458D6144B25950D" docLanguage="en" docName="MycoKeys 48: 1-40" docOrigin="MycoKeys 48" docSource="http://dx.doi.org/10.3897/mycokeys.48.30350" docTitle="Milesina woodwardiana Buchheit &amp; M. Scholler, sp. nov." docType="treatment" docVersion="4" lastPageNumber="1" masterDocId="FF8DFFF6B54CFFDEFFF350079F2D3842" masterDocTitle="Species identification of European forest pathogens of the genus Milesina (Pucciniales) using urediniospore morphology and molecular barcoding including M. woodwardiana sp. nov." masterLastPageNumber="40" masterPageNumber="1" pageNumber="1" updateTime="1668136175467" updateUser="ExternalLinkService">
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<mods:titleInfo>
<mods:title>Species identification of European forest pathogens of the genus Milesina (Pucciniales) using urediniospore morphology and molecular barcoding including M. woodwardiana sp. nov.</mods:title>
</mods:titleInfo>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Bubner, Ben</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Buchheit, Ramona</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Friedrich, Frank</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Kummer, Volker</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Scholler, Markus</mods:namePart>
</mods:name>
<mods:typeOfResource>text</mods:typeOfResource>
<mods:relatedItem type="host">
<mods:titleInfo>
<mods:title>MycoKeys</mods:title>
</mods:titleInfo>
<mods:part>
<mods:date>2019</mods:date>
<mods:detail type="volume">
<mods:number>48</mods:number>
</mods:detail>
<mods:extent unit="page">
<mods:start>1</mods:start>
<mods:end>40</mods:end>
</mods:extent>
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<mods:location>
<mods:url>http://dx.doi.org/10.3897/mycokeys.48.30350</mods:url>
</mods:location>
<mods:classification>journal article</mods:classification>
<mods:identifier type="DOI">http://dx.doi.org/10.3897/mycokeys.48.30350</mods:identifier>
<mods:identifier type="Pensoft-Pub">1314-4049--1</mods:identifier>
</mods:mods>
<treatment ID-GBIF-Taxon="154473652" LSID="urn:lsid:plazi:treatment:150FFBF2FD44281B0458D6144B25950D" httpUri="http://treatment.plazi.org/id/150FFBF2FD44281B0458D6144B25950D" lastPageNumber="1" pageId="0" pageNumber="1">
<subSubSection pageId="0" pageNumber="1" type="nomenclature">
<paragraph pageId="0" pageNumber="1">
<taxonomicName LSID="829596" authority="Buchheit &amp; M. Scholler" class="Pucciniomycetes" family="Pucciniastraceae" genus="Milesina" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Milesina woodwardiana" order="Pucciniales" pageId="0" pageNumber="1" phylum="Basidiomycota" rank="species" species="woodwardiana">Milesina woodwardiana Buchheit &amp; M. Scholler</taxonomicName>
<taxonomicNameLabel pageId="0" pageNumber="1">sp. nov.</taxonomicNameLabel>
Figure 7
<normalizedToken originalValue="af">a-f</normalizedToken>
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="1" type="holotype">
<paragraph pageId="0" pageNumber="1">Holotype.</paragraph>
<paragraph pageId="0" pageNumber="1">
<taxonomicName class="Polypodiopsida" family="Blechnaceae" genus="Woodwardia" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Woodwardia radicans" order="Polypodiales" pageId="0" pageNumber="1" phylum="Tracheophyta" rank="species" species="radicans">Woodwardia radicans</taxonomicName>
(L.) Sm., Spain, Islas Canarias, La Palma, Cubo de la Galga, ca. 2.5 km SW parking place at coastal highway W San
<normalizedToken originalValue="Bartolomé">Bartolome</normalizedToken>
, wayside in Laurosilva, 11 Aug 2017, V. Kummer (KR-M-0049033).
</paragraph>
<paragraph pageId="0" pageNumber="1">
Further specimens examined (paratypes)Spain, Islas Canarias: La Palma, Cubo de la Galga, ca. 1.2 km SW of parking lot at coastal highway W San
<normalizedToken originalValue="Bartolomé">Bartolome</normalizedToken>
, wayside in Laurosilva, 16 Aug 2015, V. Kummer, II (KR, KR-M-0048787); La Palma, Cubo de la Galga, ca. 1.2 km SW parking lot at coastal highway W San
<normalizedToken originalValue="Bartolomé">Bartolome</normalizedToken>
, near waypoint 6, wayside in Laurosilva, 11 Aug 2017, V. Kummer, II (KR, KR-M-0049036); La Palma, Cubo de la Galga, ca. 1.25 km SW parking lot at coastal highway W San
<normalizedToken originalValue="Bartolomé">Bartolome</normalizedToken>
, between waypoint 6 and 7, wayside in Laurosilva, 11 Aug 2017, V. Kummer, II (KR, KR-M-0049034).
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="1" type="description">
<paragraph pageId="0" pageNumber="1">Description.</paragraph>
<paragraph pageId="0" pageNumber="1">
Spermogonia (0), aecia (I), telia (III) and basidia (IV) unknown. Uredinia hypophyllous, subepidermal, statistically distributed; sori round, wart-like elevations, 0.1-0.3 mm in diam., covered by brownish or yellow-brownish epidermis, on dark necrotic plant tissue margined by nerves, never on nerves directly, sori opening pore-like; peridium hemispheric, peridial cells colourless, about 7.5-25.0
<normalizedToken originalValue="×">x</normalizedToken>
7.5-10
<normalizedToken originalValue="µm">µm</normalizedToken>
, upper peridial cells more or less isodiametrical and lateral peridial cells elongated; urediniospores hyaline, ellipsoidal to obovoidal, sometimes subglobose to irregular, 25.5-46.5
<normalizedToken originalValue="×">x</normalizedToken>
15.0-25.0
<normalizedToken originalValue="µm">µm</normalizedToken>
, mostly 30.0-37.5
<normalizedToken originalValue="×">x</normalizedToken>
17.5-22.5
<normalizedToken originalValue="µm">µm</normalizedToken>
; cell wall thin, 0.5-1.2
<normalizedToken originalValue="µm">µm</normalizedToken>
, mostly 0.5-1.0
<normalizedToken originalValue="µm">µm</normalizedToken>
thick, densely echinulate without spine-free areas, densest at spore base, spines 2.0-3.2
<normalizedToken originalValue="µm">µm</normalizedToken>
long, mostly 3.0
<normalizedToken originalValue="µm">µm</normalizedToken>
long, slightly irregularly distributed, spines orientated in different directions, dense basal spines typically directed toward spore pedicel, distance between spines bases 0.5-5.0
<normalizedToken originalValue="µm">µm</normalizedToken>
, mostly 2.0-4.0
<normalizedToken originalValue="µm">µm</normalizedToken>
, spine base 0.6-1.3
<normalizedToken originalValue="µm">µm</normalizedToken>
, mostly around 1
<normalizedToken originalValue="µm">µm</normalizedToken>
; spore pedicel often laterally or semilaterally inserted, short and wide, 5.5-14
<normalizedToken originalValue="×">x</normalizedToken>
12.5-15.5
<normalizedToken originalValue="µm">µm</normalizedToken>
; germ pores scattered, 8-19 (21), mostly 10-14, 1.3-3.0
<normalizedToken originalValue="µm">µm</normalizedToken>
, mostly 2.0-3.0
<normalizedToken originalValue="µm">µm</normalizedToken>
diam.,
<normalizedToken originalValue="Ø">O</normalizedToken>
2.4
<normalizedToken originalValue="µm">µm</normalizedToken>
diam.; germ tubes septate, may develop simultaneously in one spore.
</paragraph>
<caption pageId="0" pageNumber="1">
<paragraph pageId="0" pageNumber="1">
Figure 7. Spore morphology and symptoms on fern fronds of
<taxonomicName class="Pucciniomycetes" family="Pucciniastraceae" genus="Milesina" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Milesina woodwardiana" order="Pucciniales" pageId="0" pageNumber="1" phylum="Basidiomycota" rank="species" species="woodwardiana">Milesina woodwardiana</taxonomicName>
sp. nov. a Fronds of the host
<taxonomicName class="Polypodiopsida" family="Blechnaceae" genus="Woodwardia" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Woodwardia radicans" order="Polypodiales" pageId="0" pageNumber="1" phylum="Tracheophyta" rank="species" species="radicans">Woodwardia radicans</taxonomicName>
at the collection site in La Palma. Dark spots indicate areas where sori are formed on the underside (La Palma, Cubo de la Galga, ca. 1.2 km SW of parking lot W San
<normalizedToken originalValue="Bartolomé">Bartolome</normalizedToken>
, 11 Aug 2017) b Host leaf with uredinia. Sori (arrows) are restricted to areas between leaf veins (KR-M-0048787, dissecting microscope) c Transverse section of uredinium E=epidermis, P=peridial cells, U=urediniospore, M=mesophyll of host plant (KR-M-0048787, LM, interference contrast) d Urediniospores with long echinulae (KR-M-0049036, paratype, SEM) e Urediniospores, cracked, without plasma, germ pores scattered (KR-M-0049033, paratype; LM, phase contrast) f Germinating urediniospores, arrows point to germ tubes (KR-M-0049033, paratype, LM, phase contrast).
</paragraph>
</caption>
</subSubSection>
<subSubSection pageId="0" pageNumber="1" type="distribution">
<paragraph pageId="0" pageNumber="1">Distribution.</paragraph>
<paragraph pageId="0" pageNumber="1">The species is only known north-eastern La Palma, Islas Canarias, Spain.</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="1" type="etymology">
<paragraph pageId="0" pageNumber="1">Etymology.</paragraph>
<paragraph pageId="0" pageNumber="1">
Referring to the English botanist Thomas Jenkinson Woodward (1745 - 1820) and the host plant
<taxonomicName class="Polypodiopsida" family="Blechnaceae" genus="Woodwardia" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Woodwardia radicans" order="Polypodiales" pageId="0" pageNumber="1" phylum="Tracheophyta" rank="species" species="radicans">Woodwardia radicans</taxonomicName>
named after him.
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="1" type="comments">
<paragraph pageId="0" pageNumber="1">Comment.</paragraph>
<paragraph pageId="0" pageNumber="1">
This species differs from
<taxonomicName lsidName="M. blechni" pageId="0" pageNumber="1" rank="species" species="blechni">M. blechni</taxonomicName>
by the telial host plant genus (
<taxonomicName class="Gastropoda" family="Scissurellidae" genus="Woodwardia" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Woodwardia" order="Lepetellida" pageId="0" pageNumber="1" phylum="Mollusca" rank="genus">Woodwardia</taxonomicName>
), by a higher number of germ pores/spore, longer spines and irregular spine orientation.
<taxonomicName class="Pucciniomycetes" family="Pucciniastraceae" genus="Milesina" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Milesina woodwardiana" order="Pucciniales" pageId="0" pageNumber="1" phylum="Basidiomycota" rank="species" species="woodwardiana">Milesina woodwardiana</taxonomicName>
is the first
<taxonomicName class="Pucciniomycetes" family="Pucciniastraceae" genus="Milesina" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Milesina" order="Pucciniales" pageId="0" pageNumber="1" phylum="Basidiomycota" rank="genus">Milesina</taxonomicName>
species known on
<taxonomicName class="Gastropoda" family="Scissurellidae" genus="Woodwardia" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Woodwardia" order="Lepetellida" pageId="0" pageNumber="1" phylum="Mollusca" rank="genus">Woodwardia</taxonomicName>
(
<bibRefCitation author="Berndt, R" journalOrPublisher="Mycological Progress" pageId="0" pageNumber="1" pagination="7 - 19" title="The rust fungi (Uredinales) on ferns in South Africa." url="https://doi.org/10.1007/s11557-007-0548-7" volume="7" year="2008">Berndt 2008</bibRefCitation>
;
<bibRefCitation author="Faull, JH" journalOrPublisher="Contributions from the Arnold Arboretum of Harvard University" pageId="0" pageNumber="1" pagination="1 - 138" title="Taxonomy and geographical distribution of the genus Milesia." url="https://doi.org/10.5962/bhl.title.152906" volume="2" year="1932">Faull 1932</bibRefCitation>
). The absence of potential aecial hosts (
<taxonomicName class="Pinopsida" family="Pinaceae" genus="Abies" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Abies" order="Pinales" pageId="0" pageNumber="1" phylum="Tracheophyta" rank="genus">Abies</taxonomicName>
spp.) in La Palma and all other Canary Islands (
<bibRefCitation author="Hohenester, A" journalOrPublisher="Eugen Ulmer, Stuttgart" pageId="0" pageNumber="1" title="Exkursionsflora fuer die Kanarischen Inseln. Mit Ausblicken auf ganz Makaronesien." year="1993">Hohenester and Welss 1993</bibRefCitation>
,
<bibRefCitation pageId="0" pageNumber="1">
<normalizedToken originalValue="Ginovés">Ginoves</normalizedToken>
et al. 2009
</bibRefCitation>
) and the non-formation of telia indicate that the species is not host-alternating in La Palma. The
<taxonomicName class="Polypodiopsida" family="Blechnaceae" genus="Woodwardia" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Woodwardia radicans" order="Polypodiales" pageId="0" pageNumber="1" phylum="Tracheophyta" rank="species" species="radicans">Woodwardia radicans</taxonomicName>
area (
<bibRefCitation author="Hohenester, A" journalOrPublisher="Eugen Ulmer, Stuttgart" pageId="0" pageNumber="1" title="Exkursionsflora fuer die Kanarischen Inseln. Mit Ausblicken auf ganz Makaronesien." year="1993">Hohenester and Welss 1993</bibRefCitation>
), however, overlaps with those of
<taxonomicName class="Pinopsida" family="Pinaceae" genus="Abies" higherTaxonomySource="CoL" infraspecific-rank="x" kingdom="Plantae" lsidName="Abies" order="Pinales" pageId="0" pageNumber="1" phylum="Tracheophyta" rank="subspecies" subspecies="borisii-regis">
Abies
<normalizedToken originalValue="×">x</normalizedToken>
borisii-regis
</taxonomicName>
,
<taxonomicName lsidName="A. cephalonica" pageId="0" pageNumber="1" rank="species" species="cephalonica">A. cephalonica</taxonomicName>
and
<taxonomicName lsidName="A. pinsapo" pageId="0" pageNumber="1" rank="species" species="pinsapo">A. pinsapo</taxonomicName>
in south-western Europe (
<bibRefCitation author="Liu, TS" journalOrPublisher="Department of Forestry, National Taiwan University, Taipeh" pageId="0" pageNumber="1" title="A Monograph of the Genus Abies." year="1971">Liu 1971</bibRefCitation>
). If the rust is present in this area, it may be possible to observe the spore stages 0 and I on
<taxonomicName class="Pinopsida" family="Pinaceae" genus="Abies" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Abies" order="Pinales" pageId="0" pageNumber="1" phylum="Tracheophyta" rank="genus">Abies</taxonomicName>
spp.
<taxonomicName class="Polypodiopsida" family="Blechnaceae" genus="Woodwardia" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Woodwardia radicans" order="Polypodiales" pageId="0" pageNumber="1" phylum="Tracheophyta" rank="species" species="radicans">Woodwardia radicans</taxonomicName>
is the only species of
<taxonomicName class="Gastropoda" family="Scissurellidae" genus="Woodwardia" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Woodwardia" order="Lepetellida" pageId="0" pageNumber="1" phylum="Mollusca" rank="genus">Woodwardia</taxonomicName>
in Europe. There are numerous other species in SE Asia and N America (
<bibRefCitation author="Li, C-X" journalOrPublisher="Palaeoworld" pageId="0" pageNumber="1" pagination="318 - 324" title="Phylogeographic history of the woodwardioid ferns, including species from the Himalayas." url="https://doi.org/10.1016/j.palwor.2014.10.004" volume="25" year="2016">Li et al. 2016</bibRefCitation>
). These areas may also coincide with the distribution area of
<taxonomicName lsidName="M. woodwardiana" pageId="0" pageNumber="1" rank="species" species="woodwardiana">M. woodwardiana</taxonomicName>
.
</paragraph>
</subSubSection>
</treatment>
</document>