treatments-xml/data/2D/2A/B3/2D2AB30DFFD0FF93A93AFCE86DE9F90C.xml

<document id="3DDCFA1779D00130A93B6577A8C72B7F" ID-CLB-Dataset="299506" ID-DOI="10.2478/jofnem-2022-0010" ID-GBIF-Dataset="a3fb06ca-aff3-4496-ae2e-929285e30b30" ID-ISSN="2640-396X" ID-PMC="PMC9260829" ID-PubMed="35860519" ID-Zenodo-Dep="12175628" IM.bibliography_approvedBy="carolina" IM.illustrations_approvedBy="carolina" IM.materialsCitations_approvedBy="carolina" IM.metadata_approvedBy="felipe" IM.tables_approvedBy="carolina" IM.taxonomicNames_approvedBy="carolina" IM.treatments_approvedBy="carolina" checkinTime="1718848692748" checkinUser="felipe" docAuthor="Le, Tho Son, Nguyen, Thi Hong Gam, Ha, Bich Hong, Huong, Bui Thi Mai, Nguyen, Thi Thu Hang, Vu, Kim Dung, Ho, Tu Cuong &amp; Wang, John" docDate="2022" docId="2D2AB30DFFD0FF93A93AFCE86DE9F90C" docLanguage="en" docName="JNematol.54.1.0010.pdf" docOrigin="Journal of Nematology 54 (1)" docSource="http://dx.doi.org/10.2478/jofnem-2022-0010" docStyle="DocumentStyle:DDD1F39569878942AA8312288C6178BF.2:JNematol.2018-.journal_article" docStyleId="DDD1F39569878942AA8312288C6178BF" docStyleName="JNematol.2018-.journal_article" docStyleVersion="2" docTitle="Caenorhabditis elegans Dougherty 1953" docType="treatment" docVersion="6" lastPageNumber="10" masterDocId="D113CB75FFD3FF9AAA0CFFEA6C1CFFC0" masterDocTitle="Reproductive Span of Caenorhabditis elegans Is Extended by Microbacterium sp." masterLastPageNumber="21" masterPageNumber="1" pageNumber="4" updateTime="1720053519886" updateUser="ExternalLinkService" zenodo-license-document="CC-BY-4.0">
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<mods:title id="B43418362017B1662199848149E4AED3">Reproductive Span of Caenorhabditis elegans Is Extended by Microbacterium sp.</mods:title>
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<mods:namePart id="244379807BB6DED82EFDE37649D498D7">Le, Tho Son</mods:namePart>
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<mods:namePart id="5C08A2C065089013CFABD3E19A1FB4F0">Nguyen, Thi Hong Gam</mods:namePart>
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<mods:namePart id="DBA217FFAADE5E1CDF24A82B87836D36">Ha, Bich Hong</mods:namePart>
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<mods:namePart id="FD0678304FAF90BE3FB6C9B2CB84C07D">Huong, Bui Thi Mai</mods:namePart>
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<mods:namePart id="93B4E06340AE88F8B1C91F2ED6763AAF">Vu, Kim Dung</mods:namePart>
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<mods:namePart id="85E62D487D1617979CEC75C0499353FA">Ho, Tu Cuong</mods:namePart>
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Isolation and screening of bacteria affecting RS of 
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<paragraph id="A53C021BFFD0FF99A93AFC8968DCFB1C" blockId="3.[821,1438,867,1916]" pageId="3" pageNumber="4">
We hypothesized that the different bacteria encountered and eaten by 
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<emphasis id="97F7DE09FFD0FF99AE6AFC6968C2FC5C" box="[1126,1246,899,924]" italics="true" pageId="3" pageNumber="4">C. elegans</emphasis>
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in the wild could affect its physiology, and in particular RS. To this end, we first isolated bacteria from rotting vegetation, substrates from which nematodes would often be found, from two national parks in 
<collectingCountry id="DD94428BFFD0FF99AF09FBE9697BFBDC" box="[1285,1383,1027,1052]" name="Vietnam" pageId="3" pageNumber="4">Vietnam</collectingCountry>
. We isolated 64 bacterial strains from 50 sampling sites in Cat Tien National Park and 45 strains from 44 sites in Cuc Phuong National Park (
<tableCitation id="E80137A0FFD0FF99AE79FB8968FBFBBC" box="[1141,1255,1123,1148]" captionStart="Table 2" captionStartId="7.[166,248,184,214]" captionTargetBox="[177,1436,268,1903]" captionText="Table 2. Total average brood and RS of tested nematode species." httpUri="http://table.plazi.org/id/F1FC5293FFD4FF9DAAAAFF52686DFF16" pageId="3" pageNumber="4" tableUuid="F1FC5293FFD4FF9DAAAAFF52686DFF16">Tables S2</tableCitation>
and S 
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). These 109 strains were qualitatively characterized based on colony size, color, thickness, and prevalence on the original screening plate (
<tableCitation id="E80137A0FFD0FF99AE40FB2968A8FB1C" box="[1100,1204,1219,1244]" captionStart="Table S" captionStartId="16.[151,233,184,214]" captionTargetBox="[162,1423,306,1882]" captionText="Table S3. Bacterial isolates with description of colonial morphology, and geography of sample sites." httpUri="http://table.plazi.org/id/F1FC5293FFC3FF8AAA9BFF526D9CFF3B" pageId="3" pageNumber="4" tableUuid="F1FC5293FFC3FF8AAA9BFF526D9CFF3B">Table S3</tableCitation>
).
</paragraph>
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Next, we used a simple 1-plate assay (see “Methods”) to screen all 109 isolates for an effect on RS of 
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<emphasis id="97F7DE09FFD0FF99A98FFAC96FE3FAFC" box="[899,1023,1315,1340]" italics="true" pageId="3" pageNumber="4">C. elegans</emphasis>
</taxonomicName>
in comparison to the 
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<emphasis id="97F7DE09FFD0FF99AF06FAC9694CFAFC" box="[1290,1360,1315,1340]" italics="true" pageId="3" pageNumber="4">E. coli</emphasis>
OP
</taxonomicName>
50 control. The RS of 
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<emphasis id="97F7DE09FFD0FF99AE13FAA96880FA9C" box="[1055,1180,1347,1372]" italics="true" pageId="3" pageNumber="4">C. elegans</emphasis>
</taxonomicName>
on OP50 was about 3–4 days. Qualitatively, 108 strains also had similar RS in the 3- to 4-day range. One strain, CFBb37, seemed to extend RS to approximately 7 days. We note that we found a nematode, 
<taxonomicName id="62837998FFD0FF99AEFCFA296F8AFA3C" class="Chromadorea" family="Rhabditidae" genus="Caenorhabditis" kingdom="Animalia" order="Rhabditida" pageId="3" pageNumber="4" phylum="Nematoda" rank="species" species="briggsae">
<emphasis id="97F7DE09FFD0FF99AEFCFA296F8AFA3C" italics="true" pageId="3" pageNumber="4">Caenorhabditis briggsae</emphasis>
</taxonomicName>
, co-occurring in the field sample containing CFBb37 (
<tableCitation id="E80137A0FFD0FF99A9A7F9E96805F9DC" box="[939,1049,1539,1564]" captionStart="Table 2" captionStartId="7.[166,248,184,214]" captionTargetBox="[177,1436,268,1903]" captionText="Table 2. Total average brood and RS of tested nematode species." httpUri="http://table.plazi.org/id/F1FC5293FFD4FF9DAAAAFF52686DFF16" pageId="3" pageNumber="4" tableUuid="F1FC5293FFD4FF9DAAAAFF52686DFF16">Table S2</tableCitation>
). We also noticed that this and six other bacterial strains reduced the body size and another delayed the onset of first brood production in 
<taxonomicName id="62837998FFD0FF99A95AF9896FCFF9BC" authorityName="Bristol" authorityYear="2022" box="[854,979,1635,1660]" class="Chromadorea" family="Rhabditidae" genus="Caenorhabditis" kingdom="Animalia" order="Rhabditida" pageId="3" pageNumber="4" phylum="Nematoda" rank="species" species="elegans">
<emphasis id="97F7DE09FFD0FF99A95AF9896FCFF9BC" box="[854,979,1635,1660]" italics="true" pageId="3" pageNumber="4">C. elegans</emphasis>
</taxonomicName>
(
<figureCitation id="3DB81E9EFFD0FF99A9EAF9896826F9BC" box="[998,1082,1635,1660]" captionStart="Figure 1" captionStartId="6.[311,379,891,916]" captionTargetBox="[153,1409,186,844]" captionTargetId="figure-416@6.[151,1412,184,848]" captionTargetPageId="6" captionText="Figure 1: RS of individual nematodes on Microbacterium sp. CFBb37 and the E. coli OP50 control. (A) C. elegans, (B) C. briggsae, (C) C. tropicalis, and (D) Protorhabditis sp. on CFBb37 had longer RS than on OP50 (P &lt;0.001, log-rank test). Full RS data in Table 2. RS, reproductive span." figureDoi="http://doi.org/10.5281/zenodo.12175630" httpUri="https://zenodo.org/record/12175630/files/figure.png" pageId="3" pageNumber="4">Fig. S1</figureCitation>
and 
<tableCitation id="E80137A0FFD0FF99AE73F98968F6F9BC" box="[1151,1258,1635,1660]" captionStart="Table 2" captionStartId="7.[166,248,184,214]" captionTargetBox="[177,1436,268,1903]" captionText="Table 2. Total average brood and RS of tested nematode species." httpUri="http://table.plazi.org/id/F1FC5293FFD4FF9DAAAAFF52686DFF16" pageId="3" pageNumber="4" tableUuid="F1FC5293FFD4FF9DAAAAFF52686DFF16">Table S2</tableCitation>
). To determine their genus or species identity we sequenced the 16S rDNA barcode for these eight bacterial strains as well as four others without obvious effects on 
<taxonomicName id="62837998FFD0FF99A93AF9096FAEF93C" authorityName="Bristol" authorityYear="2022" box="[822,946,1763,1788]" class="Chromadorea" family="Rhabditidae" genus="Caenorhabditis" kingdom="Animalia" order="Rhabditida" pageId="3" pageNumber="4" phylum="Nematoda" rank="species" species="elegans">
<emphasis id="97F7DE09FFD0FF99A93AF9096FAEF93C" box="[822,946,1763,1788]" italics="true" pageId="3" pageNumber="4">C. elegans</emphasis>
</taxonomicName>
(
<tableCitation id="E80137A0FFD0FF99A9CFF9096832F93C" box="[963,1070,1763,1788]" captionStart="Table 2" captionStartId="7.[166,248,184,214]" captionTargetBox="[177,1436,268,1903]" captionText="Table 2. Total average brood and RS of tested nematode species." httpUri="http://table.plazi.org/id/F1FC5293FFD4FF9DAAAAFF52686DFF16" pageId="3" pageNumber="4" tableUuid="F1FC5293FFD4FF9DAAAAFF52686DFF16">Table S2</tableCitation>
). This revealed that CFBb37 is a 
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<emphasis id="97F7DE09FFD0FF99A943F8E9681DF8DC" box="[847,1025,1795,1820]" italics="true" pageId="3" pageNumber="4">Microbacterium</emphasis>
sp.
</taxonomicName>
, which is in the Actinobacteria phylum. Most of the other strains belonged to the phylum 
<taxonomicName id="62837998FFD0FF99A999F8A96822F89C" box="[917,1086,1859,1884]" kingdom="Bacteria" pageId="3" pageNumber="4" phylum="Proteobacteria" rank="phylum">Proteobacteria</taxonomicName>
(
<emphasis id="97F7DE09FFD0FF99AE43F8A96841F89C" box="[1103,1117,1859,1884]" italics="true" pageId="3" pageNumber="4">n</emphasis>
= 8) while the rest were in the phyla Bacteroidetes (
<emphasis id="97F7DE09FFD0FF99AE52F8896870F8BC" box="[1118,1132,1891,1916]" italics="true" pageId="3" pageNumber="4">n</emphasis>
= 1) or Firmicutes (
<emphasis id="97F7DE09FFD0FF99AF58F889697EF8BC" box="[1364,1378,1891,1916]" italics="true" pageId="3" pageNumber="4">n</emphasis>
= 2). We focused on 
<taxonomicName id="62837998FFD7FF9EAB55FF526E26FF11" box="[345,570,184,209]" class="Actinobacteridae" family="Microbacteriaceae" genus="Microbacterium" kingdom="Bacteria" order="Actinomycetales" pageId="4" pageNumber="5" phylum="Actinobacteria" rank="species" species="undetermined">
<emphasis id="97F7DE09FFD7FF9EAB55FF526E17FF11" box="[345,523,184,209]" italics="true" pageId="4" pageNumber="5">Microbacterium</emphasis>
sp.
</taxonomicName>
CFBb37 for the rest of this study.
</paragraph>
</subSubSection>
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<heading id="FE74B577FFD7FF9EAA9BFECA6E2AFEA5" fontSize="12" level="2" pageId="4" pageNumber="5" reason="2">
<emphasis id="97F7DE09FFD7FF9EAA9BFECA6E2AFEA5" bold="true" pageId="4" pageNumber="5">
Confirmation of RS extension by 
<taxonomicName id="62837998FFD7FF9EAA82FEAD6DAAFEA5" box="[142,438,327,357]" class="Actinobacteridae" family="Microbacteriaceae" genus="Microbacterium" kingdom="Bacteria" order="Actinomycetales" pageId="4" pageNumber="5" phylum="Actinobacteria" rank="species" species="undetermined">Microbacterium sp.</taxonomicName>
CFBb37
</emphasis>
</heading>
</paragraph>
<paragraph id="A53C021BFFD7FF9EAA9BFE6B6DABFC5A" blockId="4.[151,767,385,1370]" pageId="4" pageNumber="5">
To confirm the screening results, we determined more precisely the RS of 
<taxonomicName id="62837998FFD7FF9EABC1FE4B6E55FE7A" authorityName="Bristol" authorityYear="2022" box="[461,585,417,442]" class="Chromadorea" family="Rhabditidae" genus="Caenorhabditis" kingdom="Animalia" order="Rhabditida" pageId="4" pageNumber="5" phylum="Nematoda" rank="species" species="elegans">
<emphasis id="97F7DE09FFD7FF9EABC1FE4B6E55FE7A" box="[461,585,417,442]" italics="true" pageId="4" pageNumber="5">C. elegans</emphasis>
</taxonomicName>
when fed with 
<taxonomicName id="62837998FFD7FF9EAA9BFE2B6D64FE1A" box="[151,376,449,474]" class="Actinobacteridae" family="Microbacteriaceae" genus="Microbacterium" kingdom="Bacteria" order="Actinomycetales" pageId="4" pageNumber="5" phylum="Actinobacteria" rank="species" species="undetermined">
<emphasis id="97F7DE09FFD7FF9EAA9BFE2B6D55FE1A" box="[151,329,449,474]" italics="true" pageId="4" pageNumber="5">Microbacterium</emphasis>
sp.
</taxonomicName>
CFBb37, the control OP50, and two additional identified strains (
<taxonomicName id="62837998FFD7FF9EA821FE0B6EE3FE3A" box="[557,767,481,506]" class="Gammaproteobacteria" family="Moraxellaceae" genus="Acinetobacter" kingdom="Bacteria" order="Pseudomonadales" pageId="4" pageNumber="5" phylum="Proteobacteria" rank="species" species="undetermined">
<emphasis id="97F7DE09FFD7FF9EA821FE0B6ED6FE3A" box="[557,714,481,506]" italics="true" pageId="4" pageNumber="5">Acinetobacter</emphasis>
sp.
</taxonomicName>
CFBb9 and 
<taxonomicName id="62837998FFD7FF9EAB22FDEB6DA9FDDA" box="[302,437,513,538]" class="Gammaproteobacteria" family="Enterobacteriaceae" genus="Serratia" kingdom="Bacteria" order="Enterobacteriales" pageId="4" pageNumber="5" phylum="Proteobacteria" rank="species" species="undetermined">
<emphasis id="97F7DE09FFD7FF9EAB22FDEB6D99FDDA" box="[302,389,513,538]" italics="true" pageId="4" pageNumber="5">Serratia</emphasis>
sp.
</taxonomicName>
CFBb17). In this assay we moved pools of adults (
<emphasis id="97F7DE09FFD7FF9EABC7FDCB6DC5FDFA" box="[459,473,545,570]" italics="true" pageId="4" pageNumber="5">n</emphasis>
= 6–15) to fresh plates daily, which allowed determining both the presence of brood and counting the number of brood per day. The worms growing on OP50 as well as 
<taxonomicName id="62837998FFD7FF9EA853FD6B6CA7FD7A" class="Gammaproteobacteria" family="Moraxellaceae" genus="Acinetobacter" kingdom="Bacteria" order="Pseudomonadales" pageId="4" pageNumber="5" phylum="Proteobacteria" rank="species" species="undetermined">
<emphasis id="97F7DE09FFD7FF9EA853FD6B6EE0FD5A" box="[607,764,641,666]" italics="true" pageId="4" pageNumber="5">Acinetobacter</emphasis>
sp.
</taxonomicName>
CFBb9 and 
<taxonomicName id="62837998FFD7FF9EAB55FD4B6DC2FD7A" box="[345,478,673,698]" class="Gammaproteobacteria" family="Enterobacteriaceae" genus="Serratia" kingdom="Bacteria" order="Enterobacteriales" pageId="4" pageNumber="5" phylum="Proteobacteria" rank="species" species="undetermined">
<emphasis id="97F7DE09FFD7FF9EAB55FD4B6DACFD7A" box="[345,432,673,698]" italics="true" pageId="4" pageNumber="5">Serratia</emphasis>
sp.
</taxonomicName>
CFBb17 had similar RS durations, which was 4 days (
<emphasis id="97F7DE09FFD7FF9EA81CFD2B6E3DFD1A" box="[528,545,705,730]" italics="true" pageId="4" pageNumber="5">P</emphasis>
&lt;0.001; 
<tableCitation id="E80137A0FFD7FF9EA89AFD2B6EF2FD1A" box="[662,750,705,730]" captionStart="Table 1" captionStartId="5.[142,224,453,483]" captionTargetBox="[153,1863,575,1465]" captionText="Table 1. Average daily individual brood countsa for C. elegaNS on three environmental bacterial species and the OP50 control." httpUri="http://table.plazi.org/id/F1FC5293FFD6FF9FAA82FE2F6D45FDC9" pageId="4" pageNumber="5" tableUuid="F1FC5293FFD6FF9FAA82FE2F6D45FDC9">Table 1</tableCitation>
). In contrast, worms growing on 
<taxonomicName id="62837998FFD7FF9EA810FD0B6EE3FD3A" box="[540,767,737,762]" class="Actinobacteridae" family="Microbacteriaceae" genus="Microbacterium" kingdom="Bacteria" order="Actinomycetales" pageId="4" pageNumber="5" phylum="Actinobacteria" rank="species" species="undetermined">
<emphasis id="97F7DE09FFD7FF9EA810FD0B6ED2FD3A" box="[540,718,737,762]" italics="true" pageId="4" pageNumber="5">Microbacterium</emphasis>
sp.
</taxonomicName>
CFBb37 had an RS of 8 days (
<emphasis id="97F7DE09FFD7FF9EA80CFCEB6E0DFCDA" box="[512,529,769,794]" italics="true" pageId="4" pageNumber="5">P</emphasis>
&lt;0.001), confirming the RS extension from the screen. Qualitatively, average daily brood counts appeared to decline exponentially from day 2 onward (day 2, 
<emphasis id="97F7DE09FFD7FF9EA864FC8B6E6AFCBA" box="[616,630,865,890]" italics="true" pageId="4" pageNumber="5">n</emphasis>
= 47; day 7 and later, 
<emphasis id="97F7DE09FFD7FF9EAB04FC6B6D0AFC5A" box="[264,278,897,922]" italics="true" pageId="4" pageNumber="5">n</emphasis>
&lt;4) (
<tableCitation id="E80137A0FFD7FF9EAB5AFC6B6DB4FC5A" box="[342,424,897,922]" captionStart="Table 1" captionStartId="5.[142,224,453,483]" captionTargetBox="[153,1863,575,1465]" captionText="Table 1. Average daily individual brood countsa for C. elegaNS on three environmental bacterial species and the OP50 control." httpUri="http://table.plazi.org/id/F1FC5293FFD6FF9FAA82FE2F6D45FDC9" pageId="4" pageNumber="5" tableUuid="F1FC5293FFD6FF9FAA82FE2F6D45FDC9">Table 1</tableCitation>
).
</paragraph>
<paragraph id="A53C021BFFD7FF9EAAB1FC4B6DAFFA9A" blockId="4.[151,767,385,1370]" pageId="4" pageNumber="5">
In the above assay, the number of parents per plate varied both within and among bacterial strains. While unlikely to be of major effect, we decided to eliminate the potential confounding effects of parental interactions and differing parental density by re-examining brood presence (and brood sizes, see below) using one parental worm per plate. We found that the average RS for worms growing on 
<taxonomicName id="62837998FFD7FF9EAB97FB4B6E96FB7A" box="[411,650,1185,1210]" class="Actinobacteridae" family="Microbacteriaceae" genus="Microbacterium" kingdom="Bacteria" order="Actinomycetales" pageId="4" pageNumber="5" phylum="Actinobacteria" rank="species" species="undetermined">
<emphasis id="97F7DE09FFD7FF9EAB97FB4B6E4FFB7A" box="[411,595,1185,1210]" italics="true" pageId="4" pageNumber="5">Microbacterium</emphasis>
sp.
</taxonomicName>
CFBb37 was 79% greater than on OP50 (7.29 ± 0.25 days; mean ± 1 standard error (SE) versus 4.08 ± 0.16; 
<emphasis id="97F7DE09FFD7FF9EAA9BFAEB6CB4FADA" box="[151,168,1281,1306]" italics="true" pageId="4" pageNumber="5">P</emphasis>
&lt;0.001; 
<figureCitation id="3DB81E9EFFD7FF9EAB10FAEB6DCDFADA" box="[284,465,1281,1306]" captionStart="Figure 1" captionStartId="6.[311,379,891,916]" captionTargetBox="[153,1409,186,844]" captionTargetId="figure-416@6.[151,1412,184,848]" captionTargetPageId="6" captionText="Figure 1: RS of individual nematodes on Microbacterium sp. CFBb37 and the E. coli OP50 control. (A) C. elegans, (B) C. briggsae, (C) C. tropicalis, and (D) Protorhabditis sp. on CFBb37 had longer RS than on OP50 (P &lt;0.001, log-rank test). Full RS data in Table 2. RS, reproductive span." figureDoi="http://doi.org/10.5281/zenodo.12175630" httpUri="https://zenodo.org/record/12175630/files/figure.png" pageId="4" pageNumber="5">Fig. 1A and S1</figureCitation>
; 
<tableCitation id="E80137A0FFD7FF9EABECFAEB6E26FADA" box="[480,570,1281,1306]" captionStart="Table 2" captionStartId="7.[166,248,184,214]" captionTargetBox="[177,1436,268,1903]" captionText="Table 2. Total average brood and RS of tested nematode species." httpUri="http://table.plazi.org/id/F1FC5293FFD4FF9DAAAAFF52686DFF16" pageId="4" pageNumber="5" tableUuid="F1FC5293FFD4FF9DAAAAFF52686DFF16">Table 2</tableCitation>
). The combined data clearly indicate that CFBb37 induces RS extension in 
<taxonomicName id="62837998FFD7FF9EAB23FAAB6DB0FA9A" authorityName="Bristol" authorityYear="2022" box="[303,428,1345,1370]" class="Chromadorea" family="Rhabditidae" genus="Caenorhabditis" kingdom="Animalia" order="Rhabditida" pageId="4" pageNumber="5" phylum="Nematoda" rank="species" species="elegans">
<emphasis id="97F7DE09FFD7FF9EAB23FAAB6DB0FA9A" box="[303,428,1345,1370]" italics="true" pageId="4" pageNumber="5">C. elegans</emphasis>
</taxonomicName>
.
</paragraph>
<paragraph id="A53C021BFFD7FF9EAA9BFA606D28FA0E" blockId="4.[151,667,1418,1486]" pageId="4" pageNumber="5">
<heading id="FE74B577FFD7FF9EAA9BFA606D28FA0E" fontSize="12" level="2" pageId="4" pageNumber="5" reason="2">
<emphasis id="97F7DE09FFD7FF9EAA9BFA606D28FA0E" bold="true" pageId="4" pageNumber="5">
Daily count of eggs in the uterus of 
<taxonomicName id="62837998FFD7FF9EAA9BFA5A6D28FA0E" authorityName="Bristol" authorityYear="2022" box="[151,308,1456,1486]" class="Chromadorea" family="Rhabditidae" genus="Caenorhabditis" kingdom="Animalia" order="Rhabditida" pageId="4" pageNumber="5" phylum="Nematoda" rank="species" species="elegans">C. elegans</taxonomicName>
</emphasis>
</heading>
</paragraph>
<paragraph id="A53C021BFFD7FF9EAA9BFA01684DFF31" blockId="4.[151,767,1515,1924]" lastBlockId="4.[807,1422,184,881]" pageId="4" pageNumber="5">
Our assays for RS above were sometimes difficult (e.g., looking for rare laid eggs near the end of the RS period) or required moving the worms to new plates everyday as well as waiting for the progeny to hatch. A simplification of the RS assay would be to directly look for eggs in the uteruses (“uterus egg assay”) of the focal adult worm at 400× magnification under a DIC compound microscope each day. The presence of at least 
<specimenCount id="B385C992FFD7FF9EAB1EF9016D6DF8C4" box="[274,369,1771,1796]" count="1" pageId="4" pageNumber="5" type="egg">one egg</specimenCount>
would indicate fertility. The lack of eggs could indicate reproductive senescence or that any eggs were recently laid. Examining many adult worms each day should help distinguish between the two possibilities, although there would still be a bias for incorrectly scoring a batch of worms (i.e., day) as reproductively senescent.
</paragraph>
<paragraph id="A53C021BFFD7FF9EA941FF126849FDD1" blockId="4.[807,1422,184,881]" pageId="4" pageNumber="5">
To test whether the uterus egg assay gives comparable results to our original RS assays, we placed 
<taxonomicName id="62837998FFD7FF9EA98EFED2681DFE91" authorityName="Bristol" authorityYear="2022" box="[898,1025,312,337]" class="Chromadorea" family="Rhabditidae" genus="Caenorhabditis" kingdom="Animalia" order="Rhabditida" pageId="4" pageNumber="5" phylum="Nematoda" rank="species" species="elegans">
<emphasis id="97F7DE09FFD7FF9EA98EFED2681DFE91" box="[898,1025,312,337]" italics="true" pageId="4" pageNumber="5">C. elegans</emphasis>
</taxonomicName>
L1s on either CFBb37 or OP50 and raised them to adulthood. Then, each day we counted the number of eggs in the uterus of 29–37 worms. For each day, if this number of eggs was greater than zero (using a one-sample 
<emphasis id="97F7DE09FFD7FF9EAF0AFE526910FE11" box="[1286,1292,440,465]" italics="true" pageId="4" pageNumber="5">t</emphasis>
-test), then we considered that day to be a fertile day (i.e., not reproductively senescent).
</paragraph>
<paragraph id="A53C021BFFD7FF9EA941FDF26973FCB1" blockId="4.[807,1422,184,881]" pageId="4" pageNumber="5">
Using this assay, the worms on CFBb37 had an RS of 8 days while those on OP50 had an RS of 4 days (
<tableCitation id="E80137A0FFD7FF9EA98BFDB26FE9FDB1" box="[903,1013,600,625]" captionStart="Table S" captionStartId="19.[142,224,517,547]" captionTargetBox="[153,1863,601,1541]" captionText="Table S5. Counts of daily uterus eggs of close nematode species." httpUri="http://table.plazi.org/id/F1FC5293FFC0FF89AA82FDEF684AFDE3" pageId="4" pageNumber="5" tableUuid="F1FC5293FFC0FF89AA82FDEF684AFDE3">Table S5</tableCitation>
). The results from counting eggs in the uteruses were qualitatively concordant with the earlier brood-based assays (7.29 and 4.08 days, respectively; 
<tableCitation id="E80137A0FFD7FF9EA9CDFD526806FD11" box="[961,1050,696,721]" captionStart="Table 2" captionStartId="7.[166,248,184,214]" captionTargetBox="[177,1436,268,1903]" captionText="Table 2. Total average brood and RS of tested nematode species." httpUri="http://table.plazi.org/id/F1FC5293FFD4FF9DAAAAFF52686DFF16" pageId="4" pageNumber="5" tableUuid="F1FC5293FFD4FF9DAAAAFF52686DFF16">Table 2</tableCitation>
). Thus, although this approach has an inherent bias for reproductive senescence (i.e., scoring shorter RS), we suggest that the simpler uterus egg assay provides a reasonably good estimate for the RS, and can distinguish differences in RS, at least for those separated by several days.
</paragraph>
<paragraph id="A53C021BFFD7FF9EA92BFC4A68AAFC7E" blockId="4.[807,1221,928,997]" box="[807,1206,928,958]" pageId="4" pageNumber="5">
<heading id="FE74B577FFD7FF9EA92BFC4A68AAFC7E" box="[807,1206,928,958]" fontSize="12" level="2" pageId="4" pageNumber="5" reason="2">
<emphasis id="97F7DE09FFD7FF9EA92BFC4A68AAFC7E" bold="true" box="[807,1206,928,958]" pageId="4" pageNumber="5">
<collectingRegion id="6747CCF9FFD7FF9EA92BFC4A6FA3FC7E" box="[807,959,928,958]" pageId="4" pageNumber="5">Reduction</collectingRegion>
of brood size by
</emphasis>
</heading>
</paragraph>
<paragraph id="A53C021BFFD7FF9EA92BFC2D68D9FC25" blockId="4.[807,1221,928,997]" box="[807,1221,967,997]" pageId="4" pageNumber="5">
<heading id="FE74B577FFD7FF9EA92BFC2D68D9FC25" box="[807,1221,967,997]" fontSize="12" level="2" pageId="4" pageNumber="5" reason="2">
<emphasis id="97F7DE09FFD7FF9EA92BFC2D68D9FC25" bold="true" box="[807,1221,967,997]" pageId="4" pageNumber="5">
<taxonomicName id="62837998FFD7FF9EA92BFC2D6859FC25" box="[807,1093,967,997]" class="Actinobacteridae" family="Microbacteriaceae" genus="Microbacterium" kingdom="Bacteria" order="Actinomycetales" pageId="4" pageNumber="5" phylum="Actinobacteria" rank="species" species="undetermined">Microbacterium sp.</taxonomicName>
CFBb37
</emphasis>
</heading>
</paragraph>
<paragraph id="A53C021BFFD7FF9EA92BFBEB6866F97A" blockId="4.[806,1424,1025,1722]" pageId="4" pageNumber="5">
While confirming the RS extension by 
<taxonomicName id="62837998FFD7FF9EAED3FBEB6F56FBFA" class="Actinobacteridae" family="Microbacteriaceae" genus="Microbacterium" kingdom="Bacteria" order="Actinomycetales" pageId="4" pageNumber="5" phylum="Actinobacteria" rank="species" species="undetermined">
<emphasis id="97F7DE09FFD7FF9EAED3FBEB6991FBDA" box="[1247,1421,1025,1050]" italics="true" pageId="4" pageNumber="5">Microbacterium</emphasis>
sp.
</taxonomicName>
CFBb37 on pools of 
<taxonomicName id="62837998FFD7FF9EAE41FBCB68DBFBFA" authorityName="Bristol" authorityYear="2022" box="[1101,1223,1057,1082]" class="Chromadorea" family="Rhabditidae" genus="Caenorhabditis" kingdom="Animalia" order="Rhabditida" pageId="4" pageNumber="5" phylum="Nematoda" rank="species" species="elegans">
<emphasis id="97F7DE09FFD7FF9EAE41FBCB68DBFBFA" box="[1101,1223,1057,1082]" italics="true" pageId="4" pageNumber="5">C. elegans</emphasis>
</taxonomicName>
, we noticed that this bacterial strain reduced the average total brood size compared with OP50 (141.6 vs 251.2; 
<emphasis id="97F7DE09FFD7FF9EAF11FB8B6932FBBA" box="[1309,1326,1121,1146]" italics="true" pageId="4" pageNumber="5">P</emphasis>
&lt;0.001; 
<tableCitation id="E80137A0FFD7FF9EA92AFB6B6F6FFB5A" box="[806,883,1153,1178]" captionStart="Table 1" captionStartId="5.[142,224,453,483]" captionTargetBox="[153,1863,575,1465]" captionText="Table 1. Average daily individual brood countsa for C. elegaNS on three environmental bacterial species and the OP50 control." httpUri="http://table.plazi.org/id/F1FC5293FFD6FF9FAA82FE2F6D45FDC9" pageId="4" pageNumber="5" tableUuid="F1FC5293FFD6FF9FAA82FE2F6D45FDC9">Table 1</tableCitation>
). As above, to remove the potential confounding effects of pooled worms, we recounted the brood sizes using one parental worm per plate. We found that the average total brood size on 
<taxonomicName id="62837998FFD7FF9EAE6CFB0B6925FB3A" box="[1120,1337,1249,1274]" class="Actinobacteridae" family="Microbacteriaceae" genus="Microbacterium" kingdom="Bacteria" order="Actinomycetales" pageId="4" pageNumber="5" phylum="Actinobacteria" rank="species" species="undetermined">
<emphasis id="97F7DE09FFD7FF9EAE6CFB0B6912FB3A" box="[1120,1294,1249,1274]" italics="true" pageId="4" pageNumber="5">Microbacterium</emphasis>
sp.
</taxonomicName>
<collectionCode id="C3929ADEFFD7FF9EAF33FB0B696EFB3A" box="[1343,1394,1249,1274]" country="Canada" httpUri="http://biocol.org/urn:lsid:biocol.org:col:13058" lsid="urn:lsid:biocol.org:col:13058" name="Northern Forestry Centre, Canadian Forest Service" pageId="4" pageNumber="5" type="Herbarium">CFB</collectionCode>
37 was less than on OP50 (137.63 mean ± 5.74 SE versus 266.28 ± 6.42; 
<emphasis id="97F7DE09FFD7FF9EA9D6FACB6FF7FAFA" box="[986,1003,1313,1338]" italics="true" pageId="4" pageNumber="5">P</emphasis>
&lt;0.001, 
<tableCitation id="E80137A0FFD7FF9EAE53FACB68A0FAFA" box="[1119,1212,1313,1338]" captionStart="Table 2" captionStartId="7.[166,248,184,214]" captionTargetBox="[177,1436,268,1903]" captionText="Table 2. Total average brood and RS of tested nematode species." httpUri="http://table.plazi.org/id/F1FC5293FFD4FF9DAAAAFF52686DFF16" pageId="4" pageNumber="5" tableUuid="F1FC5293FFD4FF9DAAAAFF52686DFF16">Table 2</tableCitation>
). Partitioning the data into daily brood sizes over the first 8 days (i.e., the number of days with data for both bacterial strains, 
<tableCitation id="E80137A0FFD7FF9EA92BFA6B6F6AFA5A" box="[807,886,1409,1434]" captionStart="Table 2" captionStartId="7.[166,248,184,214]" captionTargetBox="[177,1436,268,1903]" captionText="Table 2. Total average brood and RS of tested nematode species." httpUri="http://table.plazi.org/id/F1FC5293FFD4FF9DAAAAFF52686DFF16" pageId="4" pageNumber="5" tableUuid="F1FC5293FFD4FF9DAAAAFF52686DFF16">Table 2</tableCitation>
) revealed that worms fed with OP50 had greater brood sizes than those fed with 
<taxonomicName id="62837998FFD7FF9EAEBCFA4B6993FA7A" box="[1200,1423,1441,1466]" class="Actinobacteridae" family="Microbacteriaceae" genus="Microbacterium" kingdom="Bacteria" order="Actinomycetales" pageId="4" pageNumber="5" phylum="Actinobacteria" rank="species" species="undetermined">
<emphasis id="97F7DE09FFD7FF9EAEBCFA4B6942FA7A" box="[1200,1374,1441,1466]" italics="true" pageId="4" pageNumber="5">Microbacterium</emphasis>
sp.
</taxonomicName>
<collectionCode id="C3929ADEFFD7FF9EA92BFA2B6F45FA1A" box="[807,857,1473,1498]" country="Canada" httpUri="http://biocol.org/urn:lsid:biocol.org:col:13058" lsid="urn:lsid:biocol.org:col:13058" name="Northern Forestry Centre, Canadian Forest Service" pageId="4" pageNumber="5" type="Herbarium">CFB</collectionCode>
37 during the first 3 days (29–125 more eggs/day) while the opposite (2–19 fewer eggs/day) occurred during the last 5 days (all 
<emphasis id="97F7DE09FFD7FF9EAE47F9EB6840F9DA" box="[1099,1116,1537,1562]" italics="true" pageId="4" pageNumber="5">P</emphasis>
&lt;0.001, 
<tableCitation id="E80137A0FFD7FF9EAECFF9EB690AF9DA" box="[1219,1302,1537,1562]" captionStart="Table 2" captionStartId="7.[166,248,184,214]" captionTargetBox="[177,1436,268,1903]" captionText="Table 2. Total average brood and RS of tested nematode species." httpUri="http://table.plazi.org/id/F1FC5293FFD4FF9DAAAAFF52686DFF16" pageId="4" pageNumber="5" tableUuid="F1FC5293FFD4FF9DAAAAFF52686DFF16">Table 2</tableCitation>
). Although there were more days where worms growing on CBFb37 had greater brood sizes than OP50, the magnitude of the differences was smaller during these 5 days, explaining the lower average total brood sizes for 
<taxonomicName id="62837998FFD7FF9EA946F94B6839F97A" box="[842,1061,1697,1722]" class="Actinobacteridae" family="Microbacteriaceae" genus="Microbacterium" kingdom="Bacteria" order="Actinomycetales" pageId="4" pageNumber="5" phylum="Actinobacteria" rank="species" species="undetermined">
<emphasis id="97F7DE09FFD7FF9EA946F94B6FE4F97A" box="[842,1016,1697,1722]" italics="true" pageId="4" pageNumber="5">Microbacterium</emphasis>
sp.
</taxonomicName>
<collectionCode id="C3929ADEFFD7FF9EAE2BF94B6846F97A" box="[1063,1114,1697,1722]" country="Canada" httpUri="http://biocol.org/urn:lsid:biocol.org:col:13058" lsid="urn:lsid:biocol.org:col:13058" name="Northern Forestry Centre, Canadian Forest Service" pageId="4" pageNumber="5" type="Herbarium">CFB</collectionCode>
37.
</paragraph>
<paragraph id="A53C021BFFD7FF9EA92BF9006FCFF8EE" blockId="4.[807,1372,1770,1838]" pageId="4" pageNumber="5">
<heading id="FE74B577FFD7FF9EA92BF9006FCFF8EE" fontSize="12" level="2" pageId="4" pageNumber="5" reason="2">
<emphasis id="97F7DE09FFD7FF9EA92BF9006FCFF8EE" bold="true" pageId="4" pageNumber="5">
Lifespan extension by 
<taxonomicName id="62837998FFD7FF9EAE7FF9006F4FF8EE" class="Actinobacteridae" family="Microbacteriaceae" genus="Microbacterium" kingdom="Bacteria" order="Actinomycetales" pageId="4" pageNumber="5" phylum="Actinobacteria" rank="species" species="undetermined">Microbacterium sp.</taxonomicName>
CFBb37
</emphasis>
</heading>
</paragraph>
<paragraph id="A53C021BFFD7FF9CA92BF8A16EF7FB7C" blockId="4.[807,1421,1867,1924]" lastBlockId="6.[151,766,1027,1692]" lastPageId="6" lastPageNumber="7" pageId="4" pageNumber="5">
Bacterial diet has been shown to affect the (somatic) lifespan of 
<taxonomicName id="62837998FFD7FF9EA9A0F8816837F844" authorityName="Bristol" authorityYear="2022" box="[940,1067,1899,1924]" class="Chromadorea" family="Rhabditidae" genus="Caenorhabditis" kingdom="Animalia" order="Rhabditida" pageId="4" pageNumber="5" phylum="Nematoda" rank="species" species="elegans">
<emphasis id="97F7DE09FFD7FF9EA9A0F8816837F844" box="[940,1067,1899,1924]" italics="true" pageId="4" pageNumber="5">C. elegans</emphasis>
</taxonomicName>
in many cases (Zhang et al., 2017; 
<bibRefCitation id="C1127FEAFFD5FF9CAAD3FBE96DDDFBDC" author="Kumar, A. &amp; Baruah, A. &amp; Tomioka, M. &amp; Iino, Y. &amp; Kalita, M. C. &amp; Khan, M." box="[223,449,1027,1052]" pageId="6" pageNumber="7" pagination="1229 - 1249" refId="ref9438" refString="Kumar, A., Baruah, A., Tomioka, M., Iino, Y., Kalita, M. C., and Khan, M. 2020. Caenorhabditis elegans: A model to understand host-microbe interactions. Cellular and Molecular Life Sciences 77: 1229 - 1249." type="journal article" year="2020">Kumar et al., 2020</bibRefCitation>
). Thus, we tested whether 
<taxonomicName id="62837998FFD5FF9CAA9BFBC96D9EFBFC" box="[151,386,1059,1084]" class="Actinobacteridae" family="Microbacteriaceae" genus="Microbacterium" kingdom="Bacteria" order="Actinomycetales" pageId="6" pageNumber="7" phylum="Actinobacteria" rank="species" species="undetermined">
<emphasis id="97F7DE09FFD5FF9CAA9BFBC96D50FBFC" box="[151,332,1059,1084]" italics="true" pageId="6" pageNumber="7">Microbacterium</emphasis>
sp.
</taxonomicName>
CFBb37 could also alter the 
<taxonomicName id="62837998FFD5FF9CAA9BFBA96D0BFB9C" authorityName="Bristol" authorityYear="2022" box="[151,279,1091,1116]" class="Chromadorea" family="Rhabditidae" genus="Caenorhabditis" kingdom="Animalia" order="Rhabditida" pageId="6" pageNumber="7" phylum="Nematoda" rank="species" species="elegans">
<emphasis id="97F7DE09FFD5FF9CAA9BFBA96D0BFB9C" box="[151,279,1091,1116]" italics="true" pageId="6" pageNumber="7">C. elegans</emphasis>
</taxonomicName>
lifespan. We found that worms fed with 
<taxonomicName id="62837998FFD5FF9CAA9BFB896D67FBBC" box="[151,379,1123,1148]" class="Actinobacteridae" family="Microbacteriaceae" genus="Microbacterium" kingdom="Bacteria" order="Actinomycetales" pageId="6" pageNumber="7" phylum="Actinobacteria" rank="species" species="undetermined">
<emphasis id="97F7DE09FFD5FF9CAA9BFB896D50FBBC" box="[151,332,1123,1148]" italics="true" pageId="6" pageNumber="7">Microbacterium</emphasis>
sp.
</taxonomicName>
CFBb37 lived longer than those fed with OP50 (26.77 ± 0.86 days, mean ± 1 SE, versus 17.26 ± 0.57; 
<emphasis id="97F7DE09FFD5FF9CAB8FFB496D88FB7C" box="[387,404,1187,1212]" italics="true" pageId="6" pageNumber="7">P</emphasis>
&lt;0.001; 
<figureCitation id="3DB81E9EFFD5FF9CABF3FB496E23FB7C" box="[511,575,1187,1212]" captionStart="Figure 2" captionStartId="9.[328,399,911,936]" captionTargetBox="[173,773,186,867]" captionTargetId="figure-624@9.[173,774,184,869]" captionTargetPageId="9" captionText="Figure 2: Lifespan assays of C. elegans on Microbacterium sp. CFBb37 and the OP50 control. C. elegans grown on CFBb37 survived longer than OP50 (P &lt;0.001, log-rank test). Detailed lifespan data are in Tables S4 and S5." figureDoi="http://doi.org/10.5281/zenodo.12175632" httpUri="https://zenodo.org/record/12175632/files/figure.png" pageId="6" pageNumber="7">Fig. 2</figureCitation>
and 
<tableCitation id="E80137A0FFD5FF9CA876FB496EFDFB7C" box="[634,737,1187,1212]" captionStart="Table S" captionStartId="18.[151,233,184,214]" captionTargetBox="[162,746,343,1871]" captionText="Table S4. Counts of surviving and lifespan of C. elegans on Microbacterium sp. CFBb37 and the OP50 control." httpUri="http://table.plazi.org/id/F1FC5293FFC1FF88AA9BFF526E8DFEE1" pageId="6" pageNumber="7" tableUuid="F1FC5293FFC1FF88AA9BFF526E8DFEE1">Table S4</tableCitation>
).
</paragraph>
<caption id="F1FC5293FFD6FF9FAA82FE2F6D45FDC9" ID-Table-UUID="F1FC5293FFD6FF9FAA82FE2F6D45FDC9" httpUri="http://table.plazi.org/id/F1FC5293FFD6FF9FAA82FE2F6D45FDC9" pageId="5" pageNumber="6" startId="5.[142,224,453,483]" targetBox="[153,1863,575,1465]" targetIsTable="true" targetPageId="5" targetType="table">
<paragraph id="A53C021BFFD6FF9FAA82FE2F6D45FDC9" blockId="5.[142,1775,452,521]" pageId="5" pageNumber="6">
<emphasis id="97F7DE09FFD6FF9FAA82FE2F6F5FFE15" bold="true" box="[142,835,452,483]" pageId="5" pageNumber="6">
Table 1. Average daily individual brood 
<superScript id="52F6AF53FFD6FF9FA8D9FE2F6F5FFE15" attach="left" box="[725,835,452,483]" fontSize="7" pageId="5" pageNumber="6">countsa</superScript>
</emphasis>
for 
<taxonomicName id="62837998FFD6FF9FA972FE2F680BFE23" authorityName="Bristol" authorityYear="2022" box="[894,1047,453,483]" class="Chromadorea" family="Rhabditidae" genus="Caenorhabditis" kingdom="Animalia" order="Rhabditida" pageId="5" pageNumber="6" phylum="Nematoda" rank="species" species="elegans">
<emphasis id="97F7DE09FFD6FF9FA972FE2F680BFE23" bold="true" box="[894,1047,453,483]" italics="true" pageId="5" pageNumber="6">C. elegaNS</emphasis>
</taxonomicName>
on three environmental bacterial species and the OP50 control.
</paragraph>
</caption>
<paragraph id="A53C021BFFD6FF9FAB7EFDD56A5DFA79" pageId="5" pageNumber="6">
<table id="D783F0BBFFD60065AA95FDD56B5BFA79" box="[153,1863,575,1465]" gridcols="9" gridrows="17" pageId="5" pageNumber="6">
<tr id="1BB30059FFD60065AA95FDD56B5BFD42" box="[153,1863,575,642]" gridrow="0" pageId="5" pageNumber="6" rowspan-0="1">
<th id="58626925FFD60065AB60FDD56ED3FD42" box="[364,719,575,642]" colspan="2" colspanRight="1" gridcol="1" gridrow="0" pageId="5" pageNumber="6">
<emphasis id="97F7DE09FFD6FF9FAB7EFDD56E9CFDB6" bold="true" pageId="5" pageNumber="6">
<taxonomicName id="62837998FFD6FF9FAB7EFDD56E7EFD9A" box="[370,610,575,602]" class="Gammaproteobacteria" family="Moraxellaceae" genus="Acinetobacter" kingdom="Bacteria" order="Pseudomonadales" pageId="5" pageNumber="6" phylum="Proteobacteria" rank="species" species="undetermined">Acinetobacter sp.</taxonomicName>
CFBb9 (N = 4, n = 29) 
<superScript id="52F6AF53FFD6FF9FA87AFD8C6E9CFDB6" attach="left" box="[630,640,614,630]" fontSize="6" pageId="5" pageNumber="6">b</superScript>
</emphasis>
</th>
<th id="58626925FFD60065A8E0FDD5684DFD42" box="[748,1105,575,642]" colspan="2" colspanRight="1" gridcol="3" gridrow="0" pageId="5" pageNumber="6">
<taxonomicName id="62837998FFD6FF9FA91AFDD56FADFD9A" box="[790,945,575,602]" class="Gammaproteobacteria" family="Enterobacteriaceae" genus="Serratia" kingdom="Bacteria" order="Enterobacteriales" pageId="5" pageNumber="6" phylum="Proteobacteria" rank="species" species="undetermined">Serratia sp.</taxonomicName>
CFBb17 (N = 4, n = 39) 
<superScript id="52F6AF53FFD6FF9FA9FBFD8C681DFDB6" attach="left" box="[1015,1025,614,630]" fontSize="6" pageId="5" pageNumber="6">b</superScript>
</th>
<th id="58626925FFD60065AE63FDD569D3FD42" box="[1135,1487,575,642]" colspan="2" colspanRight="1" gridcol="5" gridrow="0" pageId="5" pageNumber="6">
<emphasis id="97F7DE09FFD6FF9FAE95FDD569A6FDB6" bold="true" pageId="5" pageNumber="6">
<taxonomicName id="62837998FFD6FF9FAE95FDD569BCFD9A" box="[1177,1440,575,602]" class="Actinobacteridae" family="Microbacteriaceae" genus="Microbacterium" kingdom="Bacteria" order="Actinomycetales" pageId="5" pageNumber="6" phylum="Actinobacteria" rank="species" species="undetermined">Microbacterium sp.</taxonomicName>
CFBb37 (N = 3, n = 37) 
<superScript id="52F6AF53FFD6FF9FAFBCFD8C69A6FDB6" attach="none" box="[1456,1466,614,630]" fontSize="6" pageId="5" pageNumber="6">b</superScript>
</emphasis>
</th>
<th id="58626925FFD60065AFE5FDD56B5BFD42" box="[1513,1863,575,642]" colspan="2" colspanRight="1" gridcol="7" gridrow="0" pageId="5" pageNumber="6">
<emphasis id="97F7DE09FFD6FF9FAC0EFDD56AE4FDB6" bold="true" pageId="5" pageNumber="6">
<taxonomicName id="62837998FFD6FF9FAC0EFDD56B15FD9A" authority="OP" authorityName="OP" box="[1538,1801,575,602]" class="Gammaproteobacteria" family="Enterobacteriaceae" genus="Escherichia" kingdom="Bacteria" order="Enterobacteriales" pageId="5" pageNumber="6" phylum="Proteobacteria" rank="species" species="coli">Escherichia coli OP</taxonomicName>
50 (N = 5, n = 33) 
<superScript id="52F6AF53FFD6FF9FACE2FD8C6AE4FDB6" attach="left" box="[1774,1784,614,630]" fontSize="6" pageId="5" pageNumber="6">b</superScript>
</emphasis>
</th>
</tr>
<tr id="1BB30059FFD60065AA95FD776B5BFCFF" box="[153,1863,669,831]" gridrow="1" pageId="5" pageNumber="6">
<th id="58626925FFD60065AA95FD776D53FCFF" box="[153,335,669,831]" gridcol="0" gridrow="1" pageId="5" pageNumber="6">
<emphasis id="97F7DE09FFD6FF9FAA95FD776D53FD78" bold="true" box="[153,335,669,696]" pageId="5" pageNumber="6">Reproductive</emphasis>
day number
</th>
<td id="58626925FFD60065AB60FD776DF1FCFF" box="[364,493,669,831]" gridcol="1" gridrow="1" pageId="5" pageNumber="6">Eggs laid per individual</td>
<td id="58626925FFD60065A807FD776ED3FCFF" box="[523,719,669,831]" gridcol="2" gridrow="1" pageId="5" pageNumber="6">Greater than zero P (one-sample t- test)</td>
<td id="58626925FFD60065A8E0FD776F71FCFF" box="[748,877,669,831]" gridcol="3" gridrow="1" pageId="5" pageNumber="6">Eggs laid per individual</td>
<td id="58626925FFD60065A980FD77684DFCFF" box="[908,1105,669,831]" gridcol="4" gridrow="1" pageId="5" pageNumber="6">Greater than zero P (one-sample t- test)</td>
<td id="58626925FFD60065AE63FD7768ECFCFF" box="[1135,1264,669,831]" gridcol="5" gridrow="1" pageId="5" pageNumber="6">
Eggs 
<emphasis id="97F7DE09FFD6FF9FAE71FD0268FFFCC3" bold="true" box="[1149,1251,744,771]" pageId="5" pageNumber="6">laid per</emphasis>
individual
</td>
<td id="58626925FFD60065AF07FD7769D3FCFF" box="[1291,1487,669,831]" gridcol="6" gridrow="1" pageId="5" pageNumber="6">
<emphasis id="97F7DE09FFD6FF9FAF14FD4669DEFD07" bold="true" box="[1304,1474,684,711]" pageId="5" pageNumber="6">Greater than</emphasis>
<emphasis id="97F7DE09FFD6FF9FAF43FD3E6996FD2F" bold="true" box="[1359,1418,724,751]" pageId="5" pageNumber="6">zero</emphasis>
<emphasis id="97F7DE09FFD6FF9FAF07FD1669D3FCD7" bold="true" box="[1291,1487,764,791]" pageId="5" pageNumber="6">P (one-sample</emphasis>
<emphasis id="97F7DE09FFD6FF9FAF48FCCE6989FCFF" bold="true" box="[1348,1429,804,831]" pageId="5" pageNumber="6">t- test)</emphasis>
</td>
<td id="58626925FFD60065AFE5FD776A76FCFF" box="[1513,1642,669,831]" gridcol="7" gridrow="1" pageId="5" pageNumber="6">Eggs laid per individual</td>
<td id="58626925FFD60065AC88FD776B5BFCFF" box="[1668,1863,669,831]" gridcol="8" gridrow="1" pageId="5" pageNumber="6">
<emphasis id="97F7DE09FFD6FF9FAC9DFD466B11FCFF" bold="true" pageId="5" pageNumber="6">Greater than zero P (one-sample t- test)</emphasis>
</td>
</tr>
<tr id="1BB30059FFD60065AA95FCB16B5BFCB3" box="[153,1863,859,883]" gridrow="2" pageId="5" pageNumber="6">
<th id="58626925FFD60065AA95FCB16D53FCB3" box="[153,335,859,883]" gridcol="0" gridrow="2" pageId="5" pageNumber="6">Day 1</th>
<td id="58626925FFD60065AB60FCB16DF1FCB3" box="[364,493,859,883]" gridcol="1" gridrow="2" pageId="5" pageNumber="6">41.24</td>
<td id="58626925FFD60065A807FCB16ED3FCB3" box="[523,719,859,883]" gridcol="2" gridrow="2" pageId="5" pageNumber="6">&lt;0.001</td>
<td id="58626925FFD60065A8E0FCB16F71FCB3" box="[748,877,859,883]" gridcol="3" gridrow="2" pageId="5" pageNumber="6">69.30</td>
<td id="58626925FFD60065A980FCB1684DFCB3" box="[908,1105,859,883]" gridcol="4" gridrow="2" pageId="5" pageNumber="6">&lt;0.001</td>
<td id="58626925FFD60065AE63FCB168ECFCB3" box="[1135,1264,859,883]" gridcol="5" gridrow="2" pageId="5" pageNumber="6">35.16</td>
<td id="58626925FFD60065AF07FCB169D3FCB3" box="[1291,1487,859,883]" gridcol="6" gridrow="2" pageId="5" pageNumber="6">&lt;0.001</td>
<td id="58626925FFD60065AFE5FCB16A76FCB3" box="[1513,1642,859,883]" gridcol="7" gridrow="2" pageId="5" pageNumber="6">151.69</td>
<td id="58626925FFD60065AC88FCB16B5BFCB3" box="[1668,1863,859,883]" gridcol="8" gridrow="2" pageId="5" pageNumber="6">&lt;0.001</td>
</tr>
<tr id="1BB30059FFD60065AA95FC696B5BFC5B" box="[153,1863,899,923]" gridrow="3" pageId="5" pageNumber="6">
<th id="58626925FFD60065AA95FC696D53FC5B" box="[153,335,899,923]" gridcol="0" gridrow="3" pageId="5" pageNumber="6">Day 2</th>
<td id="58626925FFD60065AB60FC696DF1FC5B" box="[364,493,899,923]" gridcol="1" gridrow="3" pageId="5" pageNumber="6">162.34</td>
<td id="58626925FFD60065A807FC696ED3FC5B" box="[523,719,899,923]" gridcol="2" gridrow="3" pageId="5" pageNumber="6">&lt;0.001</td>
<td id="58626925FFD60065A8E0FC696F71FC5B" box="[748,877,899,923]" gridcol="3" gridrow="3" pageId="5" pageNumber="6">165.74</td>
<td id="58626925FFD60065A980FC69684DFC5B" box="[908,1105,899,923]" gridcol="4" gridrow="3" pageId="5" pageNumber="6">&lt;0.001</td>
<td id="58626925FFD60065AE63FC6968ECFC5B" box="[1135,1264,899,923]" gridcol="5" gridrow="3" pageId="5" pageNumber="6">47.00</td>
<td id="58626925FFD60065AF07FC6969D3FC5B" box="[1291,1487,899,923]" gridcol="6" gridrow="3" pageId="5" pageNumber="6">&lt;0.001</td>
<td id="58626925FFD60065AFE5FC696A76FC5B" box="[1513,1642,899,923]" gridcol="7" gridrow="3" pageId="5" pageNumber="6">95.39</td>
<td id="58626925FFD60065AC88FC696B5BFC5B" box="[1668,1863,899,923]" gridcol="8" gridrow="3" pageId="5" pageNumber="6">&lt;0.001</td>
</tr>
<tr id="1BB30059FFD60065AA95FC406B5BFC02" box="[153,1863,938,962]" gridrow="4" pageId="5" pageNumber="6">
<th id="58626925FFD60065AA95FC406D53FC02" box="[153,335,938,962]" gridcol="0" gridrow="4" pageId="5" pageNumber="6">Day 3</th>
<td id="58626925FFD60065AB60FC406DF1FC02" box="[364,493,938,962]" gridcol="1" gridrow="4" pageId="5" pageNumber="6">44.51</td>
<td id="58626925FFD60065A807FC406ED3FC02" box="[523,719,938,962]" gridcol="2" gridrow="4" pageId="5" pageNumber="6">&lt;0.001</td>
<td id="58626925FFD60065A8E0FC406F71FC02" box="[748,877,938,962]" gridcol="3" gridrow="4" pageId="5" pageNumber="6">39.23</td>
<td id="58626925FFD60065A980FC40684DFC02" box="[908,1105,938,962]" gridcol="4" gridrow="4" pageId="5" pageNumber="6">&lt;0.001</td>
<td id="58626925FFD60065AE63FC4068ECFC02" box="[1135,1264,938,962]" gridcol="5" gridrow="4" pageId="5" pageNumber="6">24.08</td>
<td id="58626925FFD60065AF07FC4069D3FC02" box="[1291,1487,938,962]" gridcol="6" gridrow="4" pageId="5" pageNumber="6">&lt;0.001</td>
<td id="58626925FFD60065AFE5FC406A76FC02" box="[1513,1642,938,962]" gridcol="7" gridrow="4" pageId="5" pageNumber="6">2.96</td>
<td id="58626925FFD60065AC88FC406B5BFC02" box="[1668,1863,938,962]" gridcol="8" gridrow="4" pageId="5" pageNumber="6">&lt;0.001</td>
</tr>
<tr id="1BB30059FFD60065AA95FC386B5BFC2A" box="[153,1863,978,1002]" gridrow="5" pageId="5" pageNumber="6">
<th id="58626925FFD60065AA95FC386D53FC2A" box="[153,335,978,1002]" gridcol="0" gridrow="5" pageId="5" pageNumber="6">Day 4</th>
<td id="58626925FFD60065AB60FC386DF1FC2A" box="[364,493,978,1002]" gridcol="1" gridrow="5" pageId="5" pageNumber="6">2.07</td>
<td id="58626925FFD60065A807FC386ED3FC2A" box="[523,719,978,1002]" gridcol="2" gridrow="5" pageId="5" pageNumber="6">&lt;0.001</td>
<td id="58626925FFD60065A8E0FC386F71FC2A" box="[748,877,978,1002]" gridcol="3" gridrow="5" pageId="5" pageNumber="6">0.95</td>
<td id="58626925FFD60065A980FC38684DFC2A" box="[908,1105,978,1002]" gridcol="4" gridrow="5" pageId="5" pageNumber="6">&lt;0.001</td>
<td id="58626925FFD60065AE63FC3868ECFC2A" box="[1135,1264,978,1002]" gridcol="5" gridrow="5" pageId="5" pageNumber="6">13.59</td>
<td id="58626925FFD60065AF07FC3869D3FC2A" box="[1291,1487,978,1002]" gridcol="6" gridrow="5" pageId="5" pageNumber="6">&lt;0.001</td>
<td id="58626925FFD60065AFE5FC386A76FC2A" box="[1513,1642,978,1002]" gridcol="7" gridrow="5" pageId="5" pageNumber="6">1.12</td>
<td id="58626925FFD60065AC88FC386B5BFC2A" box="[1668,1863,978,1002]" gridcol="8" gridrow="5" pageId="5" pageNumber="6">&lt;0.001</td>
</tr>
<tr id="1BB30059FFD60065AA95FC136B5BFBD1" box="[153,1863,1017,1041]" gridrow="6" pageId="5" pageNumber="6" rowspan-2="1" rowspan-4="1" rowspan-8="1">
<th id="58626925FFD60065AA95FC136D53FBD1" box="[153,335,1017,1041]" gridcol="0" gridrow="6" pageId="5" pageNumber="6">Day 5</th>
<td id="58626925FFD60065AB60FC136DF1FBD1" box="[364,493,1017,1041]" gridcol="1" gridrow="6" pageId="5" pageNumber="6">0</td>
<td id="58626925FFD60065A8E0FC136F71FBD1" box="[748,877,1017,1041]" gridcol="3" gridrow="6" pageId="5" pageNumber="6">0</td>
<td id="58626925FFD60065AE63FC1368ECFBD1" box="[1135,1264,1017,1041]" gridcol="5" gridrow="6" pageId="5" pageNumber="6">8.86</td>
<td id="58626925FFD60065AF07FC1369D3FBD1" box="[1291,1487,1017,1041]" gridcol="6" gridrow="6" pageId="5" pageNumber="6">&lt;0.001</td>
<td id="58626925FFD60065AFE5FC136A76FBD1" box="[1513,1642,1017,1041]" gridcol="7" gridrow="6" pageId="5" pageNumber="6">0</td>
</tr>
<tr id="1BB30059FFD60065AA95FBCB6B5BFBF9" box="[153,1863,1057,1081]" gridrow="7" pageId="5" pageNumber="6" rowspan-2="1" rowspan-4="1" rowspan-8="1">
<th id="58626925FFD60065AA95FBCB6D53FBF9" box="[153,335,1057,1081]" gridcol="0" gridrow="7" pageId="5" pageNumber="6">Day 6</th>
<td id="58626925FFD60065AB60FBCB6DF1FBF9" box="[364,493,1057,1081]" gridcol="1" gridrow="7" pageId="5" pageNumber="6">0</td>
<td id="58626925FFD60065A8E0FBCB6F71FBF9" box="[748,877,1057,1081]" gridcol="3" gridrow="7" pageId="5" pageNumber="6">0</td>
<td id="58626925FFD60065AE63FBCB68ECFBF9" box="[1135,1264,1057,1081]" gridcol="5" gridrow="7" pageId="5" pageNumber="6">6.16</td>
<td id="58626925FFD60065AF07FBCB69D3FBF9" box="[1291,1487,1057,1081]" gridcol="6" gridrow="7" pageId="5" pageNumber="6">&lt;0.001</td>
<td id="58626925FFD60065AFE5FBCB6A76FBF9" box="[1513,1642,1057,1081]" gridcol="7" gridrow="7" pageId="5" pageNumber="6">0</td>
</tr>
<tr id="1BB30059FFD60065AA95FBA26B5BFBA0" box="[153,1863,1096,1120]" gridrow="8" pageId="5" pageNumber="6" rowspan-1="1" rowspan-2="1" rowspan-3="1" rowspan-4="1" rowspan-7="1" rowspan-8="1">
<th id="58626925FFD60065AA95FBA26D53FBA0" box="[153,335,1096,1120]" gridcol="0" gridrow="8" pageId="5" pageNumber="6">Day 7</th>
<td id="58626925FFD60065AE63FBA268ECFBA0" box="[1135,1264,1096,1120]" gridcol="5" gridrow="8" pageId="5" pageNumber="6">3.51</td>
<td id="58626925FFD60065AF07FBA269D3FBA0" box="[1291,1487,1096,1120]" gridcol="6" gridrow="8" pageId="5" pageNumber="6">&lt;0.001</td>
</tr>
<tr id="1BB30059FFD60065AA95FB856B5BFB47" box="[153,1863,1135,1159]" gridrow="9" pageId="5" pageNumber="6" rowspan-1="1" rowspan-2="1" rowspan-3="1" rowspan-4="1" rowspan-7="1" rowspan-8="1">
<th id="58626925FFD60065AA95FB856D53FB47" box="[153,335,1135,1159]" gridcol="0" gridrow="9" pageId="5" pageNumber="6">Day 8</th>
<td id="58626925FFD60065AE63FB8568ECFB47" box="[1135,1264,1135,1159]" gridcol="5" gridrow="9" pageId="5" pageNumber="6">1.49</td>
<td id="58626925FFD60065AF07FB8569D3FB47" box="[1291,1487,1135,1159]" gridcol="6" gridrow="9" pageId="5" pageNumber="6">&lt;0.001</td>
</tr>
<tr id="1BB30059FFD60065AA95FB7D6B5BFB6F" box="[153,1863,1175,1199]" gridrow="10" pageId="5" pageNumber="6" rowspan-1="1" rowspan-2="1" rowspan-3="1" rowspan-4="1" rowspan-7="1" rowspan-8="1">
<th id="58626925FFD60065AA95FB7D6D53FB6F" box="[153,335,1175,1199]" gridcol="0" gridrow="10" pageId="5" pageNumber="6">Day 9</th>
<td id="58626925FFD60065AE63FB7D68ECFB6F" box="[1135,1264,1175,1199]" gridcol="5" gridrow="10" pageId="5" pageNumber="6">1.02</td>
<td id="58626925FFD60065AF07FB7D69D3FB6F" box="[1291,1487,1175,1199]" gridcol="6" gridrow="10" pageId="5" pageNumber="6">&lt;0.001</td>
</tr>
<tr id="1BB30059FFD60065AA95FB546B5BFB16" box="[153,1863,1214,1238]" gridrow="11" pageId="5" pageNumber="6" rowspan-1="1" rowspan-2="1" rowspan-3="1" rowspan-4="1" rowspan-7="1" rowspan-8="1">
<th id="58626925FFD60065AA95FB546D53FB16" box="[153,335,1214,1238]" gridcol="0" gridrow="11" pageId="5" pageNumber="6">Day 10</th>
<td id="58626925FFD60065AE63FB5468ECFB16" box="[1135,1264,1214,1238]" gridcol="5" gridrow="11" pageId="5" pageNumber="6">0.24</td>
<td id="58626925FFD60065AF07FB5469D3FB16" box="[1291,1487,1214,1238]" gridcol="6" gridrow="11" pageId="5" pageNumber="6">&lt;0.001</td>
</tr>
<tr id="1BB30059FFD60065AA95FB0C6B5BFB3E" box="[153,1863,1254,1278]" gridrow="12" pageId="5" pageNumber="6" rowspan-1="1" rowspan-2="1" rowspan-3="1" rowspan-4="1" rowspan-7="1" rowspan-8="1">
<th id="58626925FFD60065AA95FB0C6D53FB3E" box="[153,335,1254,1278]" gridcol="0" gridrow="12" pageId="5" pageNumber="6">Day 11</th>
<td id="58626925FFD60065AE63FB0C68ECFB3E" box="[1135,1264,1254,1278]" gridcol="5" gridrow="12" pageId="5" pageNumber="6">0.51</td>
<td id="58626925FFD60065AF07FB0C69D3FB3E" box="[1291,1487,1254,1278]" gridcol="6" gridrow="12" pageId="5" pageNumber="6">&lt;0.001</td>
</tr>
<tr id="1BB30059FFD60065AA95FAE76B5BFAE5" box="[153,1863,1293,1317]" gridrow="13" pageId="5" pageNumber="6" rowspan-1="1" rowspan-2="1" rowspan-3="1" rowspan-4="1" rowspan-6="1" rowspan-7="1" rowspan-8="1">
<th id="58626925FFD60065AA95FAE76D53FAE5" box="[153,335,1293,1317]" gridcol="0" gridrow="13" pageId="5" pageNumber="6">Day 12</th>
<td id="58626925FFD60065AE63FAE768ECFAE5" box="[1135,1264,1293,1317]" gridcol="5" gridrow="13" pageId="5" pageNumber="6">0</td>
</tr>
<tr id="1BB30059FFD60065AA95FADF6B5BFA8D" box="[153,1863,1333,1357]" gridrow="14" pageId="5" pageNumber="6" rowspan-1="1" rowspan-2="1" rowspan-3="1" rowspan-4="1" rowspan-6="1" rowspan-7="1" rowspan-8="1">
<th id="58626925FFD60065AA95FADF6D53FA8D" box="[153,335,1333,1357]" gridcol="0" gridrow="14" pageId="5" pageNumber="6">Day 13</th>
<td id="58626925FFD60065AE63FADF68ECFA8D" box="[1135,1264,1333,1357]" gridcol="5" gridrow="14" pageId="5" pageNumber="6">0</td>
</tr>
<tr id="1BB30059FFD60065AA95FAB66B5BFA51" box="[153,1863,1372,1425]" gridrow="15" pageId="5" pageNumber="6" rowspan-2="1" rowspan-4="1" rowspan-6="1" rowspan-8="1">
<th id="58626925FFD60065AA95FAB66D53FA51" box="[153,335,1372,1425]" gridcol="0" gridrow="15" pageId="5" pageNumber="6">
Average brood size per 
<superScript id="52F6AF53FFD6FF9FAAF8FA936D26FA46" attach="left" box="[244,314,1400,1425]" fontSize="6" pageId="5" pageNumber="6">wormc</superScript>
</th>
<td id="58626925FFD60065AB60FAB66DF1FA51" box="[364,493,1372,1425]" gridcol="1" gridrow="15" pageId="5" pageNumber="6">250.16</td>
<td id="58626925FFD60065A8E0FAB66F71FA51" box="[748,877,1372,1425]" gridcol="3" gridrow="15" pageId="5" pageNumber="6">275.22</td>
<td id="58626925FFD60065AE63FAB668ECFA51" box="[1135,1264,1372,1425]" gridcol="5" gridrow="15" pageId="5" pageNumber="6">141.62</td>
<td id="58626925FFD60065AFE5FAB66A76FA51" box="[1513,1642,1372,1425]" gridcol="7" gridrow="15" pageId="5" pageNumber="6">251.16</td>
</tr>
<tr id="1BB30059FFD60065AA95FA4A6B5BFA79" box="[153,1863,1440,1465]" gridrow="16" pageId="5" pageNumber="6" rowspan-2="1" rowspan-4="1" rowspan-6="1" rowspan-8="1">
<th id="58626925FFD60065AA95FA4A6D53FA79" box="[153,335,1440,1465]" gridcol="0" gridrow="16" pageId="5" pageNumber="6">
RS (days) 
<superScript id="52F6AF53FFD6FF9FAB0DFA4A6D15FA6E" attach="left" box="[257,265,1440,1454]" fontSize="6" pageId="5" pageNumber="6">d</superScript>
</th>
<td id="58626925FFD60065AB60FA4A6DF1FA79" box="[364,493,1440,1465]" gridcol="1" gridrow="16" pageId="5" pageNumber="6">4</td>
<td id="58626925FFD60065A8E0FA4A6F71FA79" box="[748,877,1440,1465]" gridcol="3" gridrow="16" pageId="5" pageNumber="6">4</td>
<td id="58626925FFD60065AE63FA4A68ECFA79" box="[1135,1264,1440,1465]" gridcol="5" gridrow="16" pageId="5" pageNumber="6">11</td>
<td id="58626925FFD60065AFE5FA4A6A76FA79" box="[1513,1642,1440,1465]" gridcol="7" gridrow="16" pageId="5" pageNumber="6">3.8</td>
</tr>
</table>
</paragraph>
<subSection id="CC0C1BF0FFD6FF9FAA82FA386D96F9BA" pageId="5" pageNumber="6" type="multiple">
<paragraph id="A53C021BFFD6FF9FAA82FA386965FA2B" blockId="5.[142,1401,1490,1658]" box="[142,1401,1490,1515]" pageId="5" pageNumber="6">
<superScript id="52F6AF53FFD6FF9FAA82FA386CD4FA2B" attach="right" box="[142,200,1490,1515]" fontSize="6" pageId="5" pageNumber="6">aFirst</superScript>
, an average brood number per individual was calculated per pool, then the average of the pools was reported.
</paragraph>
<paragraph id="A53C021BFFD6FF9FAA82FA1C6F46F9CF" blockId="5.[142,1401,1490,1658]" box="[142,858,1526,1551]" pageId="5" pageNumber="6">
<superScript id="52F6AF53FFD6FF9FAA82FA1C6CB0F9CF" attach="right" box="[142,172,1526,1551]" fontSize="6" pageId="5" pageNumber="6">bN</superScript>
, number of pools tested; n, total numbers of individuals tested.
</paragraph>
<paragraph id="A53C021BFFD6FF9FAA82F9F06FCFF9F3" blockId="5.[142,1401,1490,1658]" box="[142,979,1562,1587]" pageId="5" pageNumber="6">
<superScript id="52F6AF53FFD6FF9FAA82F9F06CF3F9F3" attach="right" box="[142,239,1562,1587]" fontSize="6" pageId="5" pageNumber="6">cAverage</superScript>
brood size per worm in pools is the sum of all the daily brood sizes.
</paragraph>
<paragraph id="A53C021BFFD6FF9FAA82F9D768C5F996" blockId="5.[142,1401,1490,1658]" box="[142,1241,1597,1622]" pageId="5" pageNumber="6">
<superScript id="52F6AF53FFD6FF9FAA82F9D76CA4F996" attach="right" box="[142,184,1597,1622]" fontSize="6" pageId="5" pageNumber="6">dRS</superScript>
is the number of days with average daily individual brood counts&gt;0 based on one-sample 
<emphasis id="97F7DE09FFD6FF9FAE94F9D46883F996" box="[1176,1183,1598,1622]" italics="true" pageId="5" pageNumber="6">t</emphasis>
-test.
</paragraph>
<paragraph id="A53C021BFFD6FF9FAA82F9886D96F9BA" blockId="5.[142,1401,1490,1658]" box="[142,394,1634,1658]" pageId="5" pageNumber="6">RS, reproductive span.</paragraph>
</subSection>
<caption id="F1FC5293FFD5FF9CAB3BFC9168EBFC14" ID-DOI="http://doi.org/10.5281/zenodo.12175630" ID-Zenodo-Dep="12175630" httpUri="https://zenodo.org/record/12175630/files/figure.png" pageId="6" pageNumber="7" startId="6.[311,379,891,916]" targetBox="[153,1409,186,844]" targetPageId="6" targetType="figure">
<paragraph id="A53C021BFFD5FF9CAB3BFC916975FC54" blockId="6.[311,1398,891,980]" box="[311,1385,891,916]" pageId="6" pageNumber="7">
Figure 1: RS of individual nematodes on 
<taxonomicName id="62837998FFD5FF9CA8FEFC916FD8FC54" box="[754,964,891,916]" class="Actinobacteridae" family="Microbacteriaceae" genus="Microbacterium" kingdom="Bacteria" order="Actinomycetales" pageId="6" pageNumber="7" phylum="Actinobacteria" rank="species" species="undetermined">
<emphasis id="97F7DE09FFD5FF9CA8FEFC916F87FC54" box="[754,923,891,916]" italics="true" pageId="6" pageNumber="7">Microbacterium</emphasis>
sp.
</taxonomicName>
CFBb37 and the 
<taxonomicName id="62837998FFD5FF9CAE8BFC9168EFFC54" authority="OP" authorityName="OP" box="[1159,1267,891,916]" class="Gammaproteobacteria" family="Enterobacteriaceae" genus="Escherichia" kingdom="Bacteria" order="Enterobacteriales" pageId="6" pageNumber="7" phylum="Proteobacteria" rank="species" species="coli">
<emphasis id="97F7DE09FFD5FF9CAE8BFC9168DBFC54" box="[1159,1223,891,916]" italics="true" pageId="6" pageNumber="7">E. coli</emphasis>
OP
</taxonomicName>
50 control.
</paragraph>
<paragraph id="A53C021BFFD5FF9CAB3BFC7168EBFC14" blockId="6.[311,1398,891,980]" pageId="6" pageNumber="7">
(A) 
<taxonomicName id="62837998FFD5FF9CAB56FC716DD0FC74" authorityName="Bristol" authorityYear="2022" box="[346,460,923,948]" class="Chromadorea" family="Rhabditidae" genus="Caenorhabditis" kingdom="Animalia" order="Rhabditida" pageId="6" pageNumber="7" phylum="Nematoda" rank="species" species="elegans">
<emphasis id="97F7DE09FFD5FF9CAB56FC716DD0FC74" box="[346,460,923,948]" italics="true" pageId="6" pageNumber="7">C. elegans</emphasis>
</taxonomicName>
, (B) 
<taxonomicName id="62837998FFD5FF9CABF1FC716E65FC74" box="[509,633,923,948]" class="Chromadorea" family="Rhabditidae" genus="Caenorhabditis" kingdom="Animalia" order="Rhabditida" pageId="6" pageNumber="7" phylum="Nematoda" rank="species" species="briggsae">
<emphasis id="97F7DE09FFD5FF9CABF1FC716E65FC74" box="[509,633,923,948]" italics="true" pageId="6" pageNumber="7">C. briggsae</emphasis>
</taxonomicName>
, (C) 
<taxonomicName id="62837998FFD5FF9CA8A0FC716F35FC74" box="[684,809,923,948]" class="Chromadorea" family="Rhabditidae" genus="Caenorhabditis" kingdom="Animalia" order="Rhabditida" pageId="6" pageNumber="7" phylum="Nematoda" rank="species" species="tropicalis">
<emphasis id="97F7DE09FFD5FF9CA8A0FC716F35FC74" box="[684,809,923,948]" italics="true" pageId="6" pageNumber="7">C. tropicalis</emphasis>
</taxonomicName>
, and (D) 
<taxonomicName id="62837998FFD5FF9CA987FC716857FC74" box="[907,1099,923,948]" class="Chromadorea" family="Rhabditidae" genus="Protorhabditis" kingdom="Animalia" order="Rhabditida" pageId="6" pageNumber="7" phylum="Nematoda" rank="species" species="undetermined">
<emphasis id="97F7DE09FFD5FF9CA987FC71683DFC74" box="[907,1057,923,948]" italics="true" pageId="6" pageNumber="7">Protorhabditis</emphasis>
sp.
</taxonomicName>
on CFBb37 had longer RS than on OP50 (
<emphasis id="97F7DE09FFD5FF9CABD2FC516DF3FC14" box="[478,495,955,980]" italics="true" pageId="6" pageNumber="7">P</emphasis>
&lt;0.001, log-rank test). Full RS data in Table 2. RS, reproductive span.
</paragraph>
</caption>
<paragraph id="A53C021BFFD5FF9CAAB1FB296D92F95C" blockId="6.[151,766,1027,1692]" pageId="6" pageNumber="7">
To determine at what general “time period” lifespan extension was occurring, we partitioned the total lifetime into the time spent in the larval, reproductive, and post-reproductive periods. For 
<taxonomicName id="62837998FFD5FF9CAA9BFAA96D96FA9C" box="[151,394,1347,1372]" class="Actinobacteridae" family="Microbacteriaceae" genus="Microbacterium" kingdom="Bacteria" order="Actinomycetales" pageId="6" pageNumber="7" phylum="Actinobacteria" rank="species" species="undetermined">
<emphasis id="97F7DE09FFD5FF9CAA9BFAA96D53FA9C" box="[151,335,1347,1372]" italics="true" pageId="6" pageNumber="7">Microbacterium</emphasis>
sp.
</taxonomicName>
CFBb37, 
<taxonomicName id="62837998FFD5FF9CA816FAA96EB9FA9C" authorityName="Bristol" authorityYear="2022" box="[538,677,1347,1372]" class="Chromadorea" family="Rhabditidae" genus="Caenorhabditis" kingdom="Animalia" order="Rhabditida" pageId="6" pageNumber="7" phylum="Nematoda" rank="species" species="elegans">
<emphasis id="97F7DE09FFD5FF9CA816FAA96EB9FA9C" box="[538,677,1347,1372]" italics="true" pageId="6" pageNumber="7">C. elegans</emphasis>
</taxonomicName>
spent an average of 2.89 days as larvae, 7.29 days as reproductive adults, and 16.59 days as post-reproductive adults. On OP50, these durations were 2.05, 4.08, and 11.13 days, respectively. Thus, worms fed with CFBb37 spent longer periods of time in all three stages. This suggests that the total lifespan of the worms on CFBb37 was likely a combination of slower growth as larvae plus lifespan extension during reproductive and post-reproductive stages.
</paragraph>
<paragraph id="A53C021BFFD5FF9CAA9BF9286D1CF8C6" blockId="6.[142,647,1730,1798]" pageId="6" pageNumber="7">
<heading id="FE74B577FFD5FF9CAA9BF9286D1CF8C6" fontSize="12" level="2" pageId="6" pageNumber="7" reason="2">
<emphasis id="97F7DE09FFD5FF9CAA9BF9286D1CF8C6" bold="true" pageId="6" pageNumber="7">Extension of RS in closely related species</emphasis>
</heading>
</paragraph>
<paragraph id="A53C021BFFD5FF9CAA9BF8C86938F9DC" blockId="6.[151,767,1826,1915]" lastBlockId="6.[806,1422,1027,1916]" pageId="6" pageNumber="7">
The extension of RS by 
<taxonomicName id="62837998FFD5FF9CA802F8C86EE3F8FB" box="[526,767,1826,1851]" class="Actinobacteridae" family="Microbacteriaceae" genus="Microbacterium" kingdom="Bacteria" order="Actinomycetales" pageId="6" pageNumber="7" phylum="Actinobacteria" rank="species" species="undetermined">
<emphasis id="97F7DE09FFD5FF9CA802F8C86EDCF8FB" box="[526,704,1826,1851]" italics="true" pageId="6" pageNumber="7">Microbacterium</emphasis>
sp.
</taxonomicName>
CBFb37 (compared with 
<taxonomicName id="62837998FFD5FF9CABCDF8A86E2BF89B" authority="OP" authorityName="OP" box="[449,567,1858,1883]" class="Gammaproteobacteria" family="Enterobacteriaceae" genus="Escherichia" kingdom="Bacteria" order="Enterobacteriales" pageId="6" pageNumber="7" phylum="Proteobacteria" rank="species" species="coli">
<emphasis id="97F7DE09FFD5FF9CABCDF8A86E1BF89B" box="[449,519,1858,1883]" italics="true" pageId="6" pageNumber="7">E. coli</emphasis>
OP
</taxonomicName>
50) in 
<taxonomicName id="62837998FFD5FF9CA88EF8A86EE1F89B" authorityName="Bristol" authorityYear="2022" box="[642,765,1858,1883]" class="Chromadorea" family="Rhabditidae" genus="Caenorhabditis" kingdom="Animalia" order="Rhabditida" pageId="6" pageNumber="7" phylum="Nematoda" rank="species" species="elegans">
<emphasis id="97F7DE09FFD5FF9CA88EF8A86EE1F89B" box="[642,765,1858,1883]" italics="true" pageId="6" pageNumber="7">C. elegans</emphasis>
</taxonomicName>
could be a species-specific effect. Alternatively, 
<taxonomicName id="62837998FFD5FF9CA92BFBE9681BFBDC" box="[807,1031,1027,1052]" class="Actinobacteridae" family="Microbacteriaceae" genus="Microbacterium" kingdom="Bacteria" order="Actinomycetales" pageId="6" pageNumber="7" phylum="Actinobacteria" rank="species" species="undetermined">
<emphasis id="97F7DE09FFD5FF9CA92BFBE96FC5FBDC" box="[807,985,1027,1052]" italics="true" pageId="6" pageNumber="7">Microbacterium</emphasis>
sp.
</taxonomicName>
CBFb37 may also extend RS in other worm species. We tested this by examining RS using both the uterus egg assay and the presence of brood assay for two additional 
<taxonomicName id="62837998FFD5FF9CAEEDFB896F9CFB5C" pageId="6" pageNumber="7">
<emphasis id="97F7DE09FFD5FF9CAEEDFB896991FBBC" box="[1249,1421,1123,1148]" italics="true" pageId="6" pageNumber="7">Caenorhabditis</emphasis>
species
</taxonomicName>
(
<taxonomicName id="62837998FFD5FF9CA990FB69686BFB5C" authority="CFB" authorityName="CFB" box="[924,1143,1155,1180]" class="Chromadorea" family="Rhabditidae" genus="Caenorhabditis" kingdom="Animalia" order="Rhabditida" pageId="6" pageNumber="7" phylum="Nematoda" rank="species" species="briggsae">
<emphasis id="97F7DE09FFD5FF9CA990FB696831FB5C" box="[924,1069,1155,1180]" italics="true" pageId="6" pageNumber="7">C. briggsae</emphasis>
CFB
</taxonomicName>
233 and 
<taxonomicName id="62837998FFD5FF9CAEF5FB696F41FB7C" authority="BRC" authorityName="BRC" class="Chromadorea" family="Rhabditidae" genus="Caenorhabditis" kingdom="Animalia" order="Rhabditida" pageId="6" pageNumber="7" phylum="Nematoda" rank="species" species="tropicalis">
<emphasis id="97F7DE09FFD5FF9CAEF5FB696991FB5C" box="[1273,1421,1155,1180]" italics="true" pageId="6" pageNumber="7">C. tropicalis</emphasis>
BRC
</taxonomicName>
20400) and another 
<taxonomicName id="62837998FFD5FF9CAE42FB4968C5FB7C" authorityName="Orley" authorityYear="1880" box="[1102,1241,1187,1212]" class="Chromadorea" family="Rhabditidae" kingdom="Animalia" order="Rhabditida" pageId="6" pageNumber="7" phylum="Nematoda" rank="family">Rhabditidae</taxonomicName>
family species, 
<taxonomicName id="62837998FFD5FF9CA925FB296FE1FB1C" box="[809,1021,1219,1244]" class="Chromadorea" family="Rhabditidae" genus="Protorhabditis" kingdom="Animalia" order="Rhabditida" pageId="6" pageNumber="7" phylum="Nematoda" rank="species" species="undetermined">
<emphasis id="97F7DE09FFD5FF9CA925FB296FDBFB1C" box="[809,967,1219,1244]" italics="true" pageId="6" pageNumber="7">Protorhabditis</emphasis>
sp.
</taxonomicName>
<collectionCode id="C3929ADEFFD5FF9CAE1DFB296854FB1C" box="[1041,1096,1219,1244]" country="Canada" httpUri="http://biocol.org/urn:lsid:biocol.org:col:13058" lsid="urn:lsid:biocol.org:col:13058" name="Northern Forestry Centre, Canadian Forest Service" pageId="6" pageNumber="7" type="Herbarium">CFB</collectionCode>
231. 
<taxonomicName id="62837998FFD5FF9CAE85FB29697EFB1C" authority="CFB" authorityName="CFB" box="[1161,1378,1219,1244]" class="Chromadorea" family="Rhabditidae" genus="Caenorhabditis" kingdom="Animalia" order="Rhabditida" pageId="6" pageNumber="7" phylum="Nematoda" rank="species" species="briggsae">
<emphasis id="97F7DE09FFD5FF9CAE85FB296906FB1C" box="[1161,1306,1219,1244]" italics="true" pageId="6" pageNumber="7">C. briggsae</emphasis>
CFB
</taxonomicName>
233 and 
<taxonomicName id="62837998FFD5FF9CA96DFB09682DFB3C" box="[865,1073,1251,1276]" class="Chromadorea" family="Rhabditidae" genus="Protorhabditis" kingdom="Animalia" order="Rhabditida" pageId="6" pageNumber="7" phylum="Nematoda" rank="species" species="undetermined">
<emphasis id="97F7DE09FFD5FF9CA96DFB096FE3FB3C" box="[865,1023,1251,1276]" italics="true" pageId="6" pageNumber="7">Protorhabditis</emphasis>
sp.
</taxonomicName>
<collectionCode id="C3929ADEFFD5FF9CAE33FB096869FB3C" box="[1087,1141,1251,1276]" country="Canada" httpUri="http://biocol.org/urn:lsid:biocol.org:col:13058" lsid="urn:lsid:biocol.org:col:13058" name="Northern Forestry Centre, Canadian Forest Service" pageId="6" pageNumber="7" type="Herbarium">CFB</collectionCode>
231 were isolated from two different field samples that were each also the source of two bacterial strains (
<tableCitation id="E80137A0FFD5FF9CAEA9FAC96900FAFC" box="[1189,1308,1315,1340]" captionStart="Table 2" captionStartId="7.[166,248,184,214]" captionTargetBox="[177,1436,268,1903]" captionText="Table 2. Total average brood and RS of tested nematode species." httpUri="http://table.plazi.org/id/F1FC5293FFD4FF9DAAAAFF52686DFF16" pageId="6" pageNumber="7" tableUuid="F1FC5293FFD4FF9DAAAAFF52686DFF16">Tables S2</tableCitation>
and S 
<tableCitation id="E80137A0FFD5FF9CAF7FFAC9699EFAFC" box="[1395,1410,1315,1340]" captionStart="Table S" captionStartId="16.[151,233,184,214]" captionTargetBox="[162,1423,306,1882]" captionText="Table S3. Bacterial isolates with description of colonial morphology, and geography of sample sites." httpUri="http://table.plazi.org/id/F1FC5293FFC3FF8AAA9BFF526D9CFF3B" pageId="6" pageNumber="7" tableUuid="F1FC5293FFC3FF8AAA9BFF526D9CFF3B">3</tableCitation>
). The sexual system of 
<taxonomicName id="62837998FFD5FF9CAE47FAA96902FA9C" box="[1099,1310,1347,1372]" class="Chromadorea" family="Rhabditidae" genus="Protorhabditis" kingdom="Animalia" order="Rhabditida" pageId="6" pageNumber="7" phylum="Nematoda" rank="species" species="undetermined">
<emphasis id="97F7DE09FFD5FF9CAE47FAA968F5FA9C" box="[1099,1257,1347,1372]" italics="true" pageId="6" pageNumber="7">Protorhabditis</emphasis>
sp.
</taxonomicName>
<collectionCode id="C3929ADEFFD5FF9CAF23FAA96979FA9C" box="[1327,1381,1347,1372]" country="Canada" httpUri="http://biocol.org/urn:lsid:biocol.org:col:13058" lsid="urn:lsid:biocol.org:col:13058" name="Northern Forestry Centre, Canadian Forest Service" pageId="6" pageNumber="7" type="Herbarium">CFB</collectionCode>
231 could be self-fertile hermaphrodites, because their spermathecae contain sperm (
<figureCitation id="3DB81E9EFFD5FF9CAEA5FA696919FA5C" box="[1193,1285,1411,1436]" captionStart="Figure 2" captionStartId="9.[328,399,911,936]" captionTargetBox="[173,773,186,867]" captionTargetId="figure-624@9.[173,774,184,869]" captionTargetPageId="9" captionText="Figure 2: Lifespan assays of C. elegans on Microbacterium sp. CFBb37 and the OP50 control. C. elegans grown on CFBb37 survived longer than OP50 (P &lt;0.001, log-rank test). Detailed lifespan data are in Tables S4 and S5." figureDoi="http://doi.org/10.5281/zenodo.12175632" httpUri="https://zenodo.org/record/12175632/files/figure.png" pageId="6" pageNumber="7">Fig. S2</figureCitation>
). However, other parthenogenetic 
<taxonomicName id="62837998FFD5FF9CAE48FA496950FA7C" box="[1092,1356,1443,1468]" pageId="6" pageNumber="7">
<emphasis id="97F7DE09FFD5FF9CAE48FA4968FEFA7C" box="[1092,1250,1443,1468]" italics="true" pageId="6" pageNumber="7">Protorhabditis</emphasis>
species
</taxonomicName>
also produce sperm (
<bibRefCitation id="C1127FEAFFD5FF9CA9E6FA2968A3FA1C" author="Fradin, H. &amp; Kiontke, K. &amp; Zegar, C. &amp; Gutwein, M. &amp; Lucas, J. &amp; Kovtun, M. &amp; Corcoran, D. L. &amp; Baugh, L. R. &amp; Fitch, D. H. A. &amp; Piano, F. &amp; Gunsalus, K. C." box="[1002,1215,1475,1500]" pageId="6" pageNumber="7" pagination="2928 - 2939" refId="ref8743" refString="Fradin, H., Kiontke, K., Zegar, C., Gutwein, M., Lucas, J., Kovtun, M., Corcoran, D. L., Baugh, L. R., Fitch, D. H. A., Piano, F., and Gunsalus, K. C. 2017. Genome architecture and evolution of a unichromosomal asexual nematode. Current Biology 27: 2928 - 2939." type="journal article" year="2017">Fradin et al., 2017</bibRefCitation>
; 
<bibRefCitation id="C1127FEAFFD5FF9CAEC0FA296F7FFA3C" author="Grosmaire, M. &amp; Launay, C. &amp; Siegwald, M. &amp; Brugiere, T. &amp; Estrada-Virrueta, L. &amp; Berger, D. &amp; Burny, C. &amp; Modolo, L. &amp; Blaxter, M. &amp; Meister, P. &amp; Felix, M. A. &amp; Gouyon, P. H. &amp; Delattre, M." pageId="6" pageNumber="7" pagination="1210 - 1213" refId="ref8931" refString="Grosmaire, M., Launay, C., Siegwald, M., Brugiere, T., Estrada-Virrueta, L., Berger, D., Burny, C., Modolo, L., Blaxter, M., Meister, P., Felix, M. A., Gouyon, P. H., and Delattre, M. 2019. Males as somatic investment in a parthenogenetic nematode. Science 363: 1210 - 1213." type="journal article" year="2019">Grosmaire et al., 2019</bibRefCitation>
), so additional genetic analysis will be needed to determine the sexual system of this species.
</paragraph>
<paragraph id="A53C021BFFD5FF9CA941F9C96F6DF8BC" blockId="6.[806,1422,1027,1916]" pageId="6" pageNumber="7">
For all three species and for both assays, we found that feeding on 
<taxonomicName id="62837998FFD5FF9CA9D4F9A968A9F99C" box="[984,1205,1603,1628]" class="Actinobacteridae" family="Microbacteriaceae" genus="Microbacterium" kingdom="Bacteria" order="Actinomycetales" pageId="6" pageNumber="7" phylum="Actinobacteria" rank="species" species="undetermined">
<emphasis id="97F7DE09FFD5FF9CA9D4F9A96896F99C" box="[984,1162,1603,1628]" italics="true" pageId="6" pageNumber="7">Microbacterium</emphasis>
sp.
</taxonomicName>
BCFb37 extended the RS compared with OP50 (all 
<emphasis id="97F7DE09FFD5FF9CAEA3F98968DCF9BC" box="[1199,1216,1635,1660]" italics="true" pageId="6" pageNumber="7">P</emphasis>
&lt;0.001, log-rank test; 
<figureCitation id="3DB81E9EFFD5FF9CA96AF9696872F95C" box="[870,1134,1667,1692]" captionStart="Figure 1" captionStartId="6.[311,379,891,916]" captionTargetBox="[153,1409,186,844]" captionTargetId="figure-416@6.[151,1412,184,848]" captionTargetPageId="6" captionText="Figure 1: RS of individual nematodes on Microbacterium sp. CFBb37 and the E. coli OP50 control. (A) C. elegans, (B) C. briggsae, (C) C. tropicalis, and (D) Protorhabditis sp. on CFBb37 had longer RS than on OP50 (P &lt;0.001, log-rank test). Full RS data in Table 2. RS, reproductive span." figureDoi="http://doi.org/10.5281/zenodo.12175630" httpUri="https://zenodo.org/record/12175630/files/figure.png" pageId="6" pageNumber="7">Figures 1B–D and S1</figureCitation>
; 
<tableCitation id="E80137A0FFD5FF9CAE8CF96968FBF95C" box="[1152,1255,1667,1692]" captionStart="Table 2" captionStartId="7.[166,248,184,214]" captionTargetBox="[177,1436,268,1903]" captionText="Table 2. Total average brood and RS of tested nematode species." httpUri="http://table.plazi.org/id/F1FC5293FFD4FF9DAAAAFF52686DFF16" pageId="6" pageNumber="7" tableUuid="F1FC5293FFD4FF9DAAAAFF52686DFF16">Tables 2</tableCitation>
and S 
<tableCitation id="E80137A0FFD5FF9CAF4CF9696953F95C" box="[1344,1359,1667,1692]" captionStart="Table S" captionStartId="19.[142,224,517,547]" captionTargetBox="[153,1863,601,1541]" captionText="Table S5. Counts of daily uterus eggs of close nematode species." httpUri="http://table.plazi.org/id/F1FC5293FFC0FF89AA82FDEF684AFDE3" pageId="6" pageNumber="7" tableUuid="F1FC5293FFC0FF89AA82FDEF684AFDE3">5</tableCitation>
). For 
<taxonomicName id="62837998FFD5FF9CA92BF9496FB4F97C" box="[807,936,1699,1724]" class="Chromadorea" family="Rhabditidae" genus="Caenorhabditis" kingdom="Animalia" order="Rhabditida" pageId="6" pageNumber="7" phylum="Nematoda" rank="species" species="briggsae">
<emphasis id="97F7DE09FFD5FF9CA92BF9496FB4F97C" box="[807,936,1699,1724]" italics="true" pageId="6" pageNumber="7">C. briggsae</emphasis>
</taxonomicName>
the RS extension was 1.64 additional days (+41%), for 
<taxonomicName id="62837998FFD5FF9CA9BDF9296821F91C" box="[945,1085,1731,1756]" class="Chromadorea" family="Rhabditidae" genus="Caenorhabditis" kingdom="Animalia" order="Rhabditida" pageId="6" pageNumber="7" phylum="Nematoda" rank="species" species="tropicalis">
<emphasis id="97F7DE09FFD5FF9CA9BDF9296821F91C" box="[945,1085,1731,1756]" italics="true" pageId="6" pageNumber="7">C. tropicalis</emphasis>
</taxonomicName>
2.59 days (+109%), and for 
<taxonomicName id="62837998FFD5FF9CA92BF9096FEFF93C" box="[807,1011,1763,1788]" class="Chromadorea" family="Rhabditidae" genus="Protorhabditis" kingdom="Animalia" order="Rhabditida" pageId="6" pageNumber="7" phylum="Nematoda" rank="species" species="undetermined">
<emphasis id="97F7DE09FFD5FF9CA92BF9096FD9F93C" box="[807,965,1763,1788]" italics="true" pageId="6" pageNumber="7">Protorhabditis</emphasis>
sp.
</taxonomicName>
3.09 days (+86%). Together, these results suggest that 
<taxonomicName id="62837998FFD5FF9CAE1AF8E968EEF8DC" box="[1046,1266,1795,1820]" class="Actinobacteridae" family="Microbacteriaceae" genus="Microbacterium" kingdom="Bacteria" order="Actinomycetales" pageId="6" pageNumber="7" phylum="Actinobacteria" rank="species" species="undetermined">
<emphasis id="97F7DE09FFD5FF9CAE1AF8E968D4F8DC" box="[1046,1224,1795,1820]" italics="true" pageId="6" pageNumber="7">Microbacterium</emphasis>
sp.
</taxonomicName>
CBFb37 can likely extend RS broadly across the 
<taxonomicName id="62837998FFD5FF9CAEEDF8C96991F8FC" box="[1249,1421,1827,1852]" class="Chromadorea" family="Rhabditidae" genus="Caenorhabditis" kingdom="Animalia" order="Rhabditida" pageId="6" pageNumber="7" phylum="Nematoda" rank="genus">
<emphasis id="97F7DE09FFD5FF9CAEEDF8C96991F8FC" box="[1249,1421,1827,1852]" italics="true" pageId="6" pageNumber="7">Caenorhabditis</emphasis>
</taxonomicName>
genus and perhaps, at least, across the 
<taxonomicName id="62837998FFD5FF9CAF0EF8A96991F89C" authorityName="Orley" authorityYear="1880" box="[1282,1421,1859,1884]" class="Chromadorea" family="Rhabditidae" kingdom="Animalia" order="Rhabditida" pageId="6" pageNumber="7" phylum="Nematoda" rank="family">Rhabditidae</taxonomicName>
family.
</paragraph>
<caption id="F1FC5293FFD4FF9DAAAAFF52686DFF16" ID-Table-UUID="F1FC5293FFD4FF9DAAAAFF52686DFF16" box="[166,1137,184,214]" httpUri="http://table.plazi.org/id/F1FC5293FFD4FF9DAAAAFF52686DFF16" pageId="7" pageNumber="8" startId="7.[166,248,184,214]" targetBox="[177,1436,268,1903]" targetIsTable="true" targetPageId="7" targetType="table">
<paragraph id="A53C021BFFD4FF9DAAAAFF52686DFF16" blockId="7.[166,1137,184,214]" box="[166,1137,184,214]" pageId="7" pageNumber="8">Table 2. Total average brood and RS of tested nematode species.</paragraph>
</caption>
<paragraph id="A53C021BFFD4FF9DAB8DFEE66935F8AF" pageId="7" pageNumber="8">
<table id="D783F0BBFFD40065AABDFEE66980F8AF" box="[177,1436,268,1903]" gridcols="6" gridrows="36" pageId="7" pageNumber="8">
<tr id="1BB30059FFD40065AABDFEE66980FEE7" box="[177,1436,268,295]" gridrow="0" pageId="7" pageNumber="8" rowspan-0="1" rowspan-2="1" rowspan-4="1">
<th id="58626925FFD40065AB8DFEE66E38FEE7" box="[385,548,268,295]" gridcol="1" gridrow="0" pageId="7" pageNumber="8">
<emphasis id="97F7DE09FFD4FF9DAB8DFEE66DD7FEE7" bold="true" box="[385,459,268,295]" pageId="7" pageNumber="8">OP50</emphasis>
</th>
<th id="58626925FFD40065A949FEE66FF4FEE7" box="[837,1000,268,295]" gridcol="3" gridrow="0" pageId="7" pageNumber="8">CFBb37</th>
<th id="58626925FFD40065AF0AFEE66980FEE7" box="[1286,1436,268,295]" gridcol="5" gridrow="0" pageId="7" pageNumber="8">
<emphasis id="97F7DE09FFD4FF9DAF0AFEE66966FEE7" bold="true" box="[1286,1402,268,295]" pageId="7" pageNumber="8">Different</emphasis>
</th>
</tr>
<tr id="1BB30059FFD40065AABDFEDE6980FE5F" box="[177,1436,308,415]" gridrow="1" pageId="7" pageNumber="8">
<th id="58626925FFD40065AABDFEDE6D7BFE5F" box="[177,359,308,415]" gridcol="0" gridrow="1" pageId="7" pageNumber="8">Reproductive day number</th>
<td id="58626925FFD40065AB8DFEDE6E38FE5F" box="[385,548,308,415]" gridcol="1" gridrow="1" pageId="7" pageNumber="8">Eggs laid per day</td>
<td id="58626925FFD40065A84CFEDE6F30FE5F" box="[576,812,308,415]" gridcol="2" gridrow="1" pageId="7" pageNumber="8">
<emphasis id="97F7DE09FFD4FF9DA84CFEBD6F30FEB2" bold="true" box="[576,812,343,370]" pageId="7" pageNumber="8">Greater than zero</emphasis>
<emphasis id="97F7DE09FFD4FF9DA84CFE956E48FE5A" bold="true" box="[576,596,383,410]" pageId="7" pageNumber="8">P</emphasis>
</td>
<td id="58626925FFD40065A949FEDE6FF4FE5F" box="[837,1000,308,415]" gridcol="3" gridrow="1" pageId="7" pageNumber="8">Eggs laid per day</td>
<td id="58626925FFD40065AE0FFEDE68ECFE5F" box="[1027,1264,308,415]" gridcol="4" gridrow="1" pageId="7" pageNumber="8">Greater than zero P</td>
<td id="58626925FFD40065AF0AFEDE6980FE5F" box="[1286,1436,308,415]" gridcol="5" gridrow="1" pageId="7" pageNumber="8">between the strains P (paired</td>
</tr>
<tr id="1BB30059FFD40065AABDFE4D6980FE07" box="[177,1436,423,455]" gridrow="2" pageId="7" pageNumber="8" rowspan-0="1">
<td id="58626925FFD40065AB8DFE4D6E38FE07" box="[385,548,423,455]" gridcol="1" gridrow="2" pageId="7" pageNumber="8">(mean ± SE)</td>
<td id="58626925FFD40065A84CFE4D6F30FE07" box="[576,812,423,455]" gridcol="2" gridrow="2" pageId="7" pageNumber="8">
<emphasis id="97F7DE09FFD4FF9DA84CFE4D6F37FE02" bold="true" box="[576,811,423,450]" pageId="7" pageNumber="8">(one-sample t- test)</emphasis>
</td>
<td id="58626925FFD40065A949FE4D6FF4FE07" box="[837,1000,423,455]" gridcol="3" gridrow="2" pageId="7" pageNumber="8">(mean ± SE)</td>
<td id="58626925FFD40065AE0FFE4D68ECFE07" box="[1027,1264,423,455]" gridcol="4" gridrow="2" pageId="7" pageNumber="8">(one-sample t- test)</td>
<td id="58626925FFD40065AF0AFE4D6980FE07" box="[1286,1436,423,455]" gridcol="5" gridrow="2" pageId="7" pageNumber="8">
<emphasis id="97F7DE09FFD4FF9DAF0AFE46694AFE07" bold="true" box="[1286,1366,428,455]" pageId="7" pageNumber="8">t- test)</emphasis>
</td>
</tr>
<tr id="1BB30059FFD40065AABDFE0E6980FE3C" box="[177,1436,484,508]" gridrow="3" pageId="7" pageNumber="8">
<th id="58626925FFD40065AABDFE0E6980FE3C" box="[177,1436,484,508]" colspan="6" colspanRight="5" gridcol="0" gridrow="3" pageId="7" pageNumber="8">
<taxonomicName id="62837998FFD4FF9DAABDFE0E6D3EFE3C" box="[177,290,484,508]" pageId="7" pageNumber="8">
<emphasis id="97F7DE09FFD4FF9DAABDFE0E6D3EFE3C" box="[177,290,484,508]" italics="true" pageId="7" pageNumber="8">C. elegans</emphasis>
</taxonomicName>
</th>
</tr>
<tr id="1BB30059FFD40065AABDFDE16980FDE3" box="[177,1436,523,547]" gridrow="4" pageId="7" pageNumber="8">
<th id="58626925FFD40065AABDFDE16D7BFDE3" box="[177,359,523,547]" gridcol="0" gridrow="4" pageId="7" pageNumber="8">Day 1</th>
<td id="58626925FFD40065AB8DFDE16E38FDE3" box="[385,548,523,547]" gridcol="1" gridrow="4" pageId="7" pageNumber="8">32.68 ± 5.04</td>
<td id="58626925FFD40065A84CFDE16F30FDE3" box="[576,812,523,547]" gridcol="2" gridrow="4" pageId="7" pageNumber="8">&lt;0.001</td>
<td id="58626925FFD40065A949FDE16FF4FDE3" box="[837,1000,523,547]" gridcol="3" gridrow="4" pageId="7" pageNumber="8">3.76 ± 1.74</td>
<td id="58626925FFD40065AE0FFDE168ECFDE3" box="[1027,1264,523,547]" gridcol="4" gridrow="4" pageId="7" pageNumber="8">&lt;0.001</td>
<td id="58626925FFD40065AF0AFDE16980FDE3" box="[1286,1436,523,547]" gridcol="5" gridrow="4" pageId="7" pageNumber="8">&lt;0.01</td>
</tr>
<tr id="1BB30059FFD40065AABDFDD96980FD8B" box="[177,1436,563,587]" gridrow="5" pageId="7" pageNumber="8">
<th id="58626925FFD40065AABDFDD96D7BFD8B" box="[177,359,563,587]" gridcol="0" gridrow="5" pageId="7" pageNumber="8">Day 2</th>
<td id="58626925FFD40065AB8DFDD96E38FD8B" box="[385,548,563,587]" gridcol="1" gridrow="5" pageId="7" pageNumber="8">149.75 ± 4.71</td>
<td id="58626925FFD40065A84CFDD96F30FD8B" box="[576,812,563,587]" gridcol="2" gridrow="5" pageId="7" pageNumber="8">&lt;0.001</td>
<td id="58626925FFD40065A949FDD96FF4FD8B" box="[837,1000,563,587]" gridcol="3" gridrow="5" pageId="7" pageNumber="8">24.64 ± 2.53</td>
<td id="58626925FFD40065AE0FFDD968ECFD8B" box="[1027,1264,563,587]" gridcol="4" gridrow="5" pageId="7" pageNumber="8">&lt;0.001</td>
<td id="58626925FFD40065AF0AFDD96980FD8B" box="[1286,1436,563,587]" gridcol="5" gridrow="5" pageId="7" pageNumber="8">&lt;0.001</td>
</tr>
<tr id="1BB30059FFD40065AABDFDB16980FDB3" box="[177,1436,603,627]" gridrow="6" pageId="7" pageNumber="8">
<th id="58626925FFD40065AABDFDB16D7BFDB3" box="[177,359,603,627]" gridcol="0" gridrow="6" pageId="7" pageNumber="8">Day 3</th>
<td id="58626925FFD40065AB8DFDB16E38FDB3" box="[385,548,603,627]" gridcol="1" gridrow="6" pageId="7" pageNumber="8">78.55 ± 6.46</td>
<td id="58626925FFD40065A84CFDB16F30FDB3" box="[576,812,603,627]" gridcol="2" gridrow="6" pageId="7" pageNumber="8">&lt;0.001</td>
<td id="58626925FFD40065A949FDB16FF4FDB3" box="[837,1000,603,627]" gridcol="3" gridrow="6" pageId="7" pageNumber="8">23.49 ± 2.12</td>
<td id="58626925FFD40065AE0FFDB168ECFDB3" box="[1027,1264,603,627]" gridcol="4" gridrow="6" pageId="7" pageNumber="8">&lt;0.001</td>
<td id="58626925FFD40065AF0AFDB16980FDB3" box="[1286,1436,603,627]" gridcol="5" gridrow="6" pageId="7" pageNumber="8">&lt;0.001</td>
</tr>
<tr id="1BB30059FFD40065AABDFD686980FD5A" box="[177,1436,642,666]" gridrow="7" pageId="7" pageNumber="8">
<th id="58626925FFD40065AABDFD686D7BFD5A" box="[177,359,642,666]" gridcol="0" gridrow="7" pageId="7" pageNumber="8">Day 4</th>
<td id="58626925FFD40065AB8DFD686E38FD5A" box="[385,548,642,666]" gridcol="1" gridrow="7" pageId="7" pageNumber="8">3.25 ± 0.81</td>
<td id="58626925FFD40065A84CFD686F30FD5A" box="[576,812,642,666]" gridcol="2" gridrow="7" pageId="7" pageNumber="8">&lt;0.001</td>
<td id="58626925FFD40065A949FD686FF4FD5A" box="[837,1000,642,666]" gridcol="3" gridrow="7" pageId="7" pageNumber="8">22.05 ± 2.31</td>
<td id="58626925FFD40065AE0FFD6868ECFD5A" box="[1027,1264,642,666]" gridcol="4" gridrow="7" pageId="7" pageNumber="8">&lt;0.001</td>
<td id="58626925FFD40065AF0AFD686980FD5A" box="[1286,1436,642,666]" gridcol="5" gridrow="7" pageId="7" pageNumber="8">&lt;0.001</td>
</tr>
<tr id="1BB30059FFD40065AABDFD406980FD02" box="[177,1436,682,706]" gridrow="8" pageId="7" pageNumber="8">
<th id="58626925FFD40065AABDFD406D7BFD02" box="[177,359,682,706]" gridcol="0" gridrow="8" pageId="7" pageNumber="8">Day 5</th>
<td id="58626925FFD40065AB8DFD406E38FD02" box="[385,548,682,706]" gridcol="1" gridrow="8" pageId="7" pageNumber="8">1.40 ± 0.77</td>
<td id="58626925FFD40065A84CFD406F30FD02" box="[576,812,682,706]" gridcol="2" gridrow="8" pageId="7" pageNumber="8">&gt;0.05</td>
<td id="58626925FFD40065A949FD406FF4FD02" box="[837,1000,682,706]" gridcol="3" gridrow="8" pageId="7" pageNumber="8">15.10 ± 1.75</td>
<td id="58626925FFD40065AE0FFD4068ECFD02" box="[1027,1264,682,706]" gridcol="4" gridrow="8" pageId="7" pageNumber="8">&lt;0.001</td>
<td id="58626925FFD40065AF0AFD406980FD02" box="[1286,1436,682,706]" gridcol="5" gridrow="8" pageId="7" pageNumber="8">&lt;0.001</td>
</tr>
<tr id="1BB30059FFD40065AABDFD3B6980FD29" box="[177,1436,721,745]" gridrow="9" pageId="7" pageNumber="8">
<th id="58626925FFD40065AABDFD3B6D7BFD29" box="[177,359,721,745]" gridcol="0" gridrow="9" pageId="7" pageNumber="8">Day 6</th>
<td id="58626925FFD40065AB8DFD3B6E38FD29" box="[385,548,721,745]" gridcol="1" gridrow="9" pageId="7" pageNumber="8">0.58 ± 0.55</td>
<td id="58626925FFD40065A84CFD3B6F30FD29" box="[576,812,721,745]" gridcol="2" gridrow="9" pageId="7" pageNumber="8">&gt;0.05</td>
<td id="58626925FFD40065A949FD3B6FF4FD29" box="[837,1000,721,745]" gridcol="3" gridrow="9" pageId="7" pageNumber="8">7.93 ± 1.14</td>
<td id="58626925FFD40065AE0FFD3B68ECFD29" box="[1027,1264,721,745]" gridcol="4" gridrow="9" pageId="7" pageNumber="8">&lt;0.001</td>
<td id="58626925FFD40065AF0AFD3B6980FD29" box="[1286,1436,721,745]" gridcol="5" gridrow="9" pageId="7" pageNumber="8">&lt;0.001</td>
</tr>
<tr id="1BB30059FFD40065AABDFD136980FCD1" box="[177,1436,761,785]" gridrow="10" pageId="7" pageNumber="8">
<th id="58626925FFD40065AABDFD136D7BFCD1" box="[177,359,761,785]" gridcol="0" gridrow="10" pageId="7" pageNumber="8">Day 7</th>
<td id="58626925FFD40065AB8DFD136E38FCD1" box="[385,548,761,785]" gridcol="1" gridrow="10" pageId="7" pageNumber="8">0.05 ± 0.03</td>
<td id="58626925FFD40065A84CFD136F30FCD1" box="[576,812,761,785]" gridcol="2" gridrow="10" pageId="7" pageNumber="8">&gt;0.05</td>
<td id="58626925FFD40065A949FD136FF4FCD1" box="[837,1000,761,785]" gridcol="3" gridrow="10" pageId="7" pageNumber="8">4.39 ± 0.79</td>
<td id="58626925FFD40065AE0FFD1368ECFCD1" box="[1027,1264,761,785]" gridcol="4" gridrow="10" pageId="7" pageNumber="8">&lt;0.001</td>
<td id="58626925FFD40065AF0AFD136980FCD1" box="[1286,1436,761,785]" gridcol="5" gridrow="10" pageId="7" pageNumber="8">&lt;0.001</td>
</tr>
<tr id="1BB30059FFD40065AABDFCCA6980FCF8" box="[177,1436,800,824]" gridrow="11" pageId="7" pageNumber="8">
<th id="58626925FFD40065AABDFCCA6D7BFCF8" box="[177,359,800,824]" gridcol="0" gridrow="11" pageId="7" pageNumber="8">Day 8</th>
<td id="58626925FFD40065AB8DFCCA6E38FCF8" box="[385,548,800,824]" gridcol="1" gridrow="11" pageId="7" pageNumber="8">0.03 ± 0.03</td>
<td id="58626925FFD40065A84CFCCA6F30FCF8" box="[576,812,800,824]" gridcol="2" gridrow="11" pageId="7" pageNumber="8">&gt;0.05</td>
<td id="58626925FFD40065A949FCCA6FF4FCF8" box="[837,1000,800,824]" gridcol="3" gridrow="11" pageId="7" pageNumber="8">1.88 ± 0.51</td>
<td id="58626925FFD40065AE0FFCCA68ECFCF8" box="[1027,1264,800,824]" gridcol="4" gridrow="11" pageId="7" pageNumber="8">&lt;0.001</td>
<td id="58626925FFD40065AF0AFCCA6980FCF8" box="[1286,1436,800,824]" gridcol="5" gridrow="11" pageId="7" pageNumber="8">&lt;0.01</td>
</tr>
<tr id="1BB30059FFD40065AABDFCAD6980FC9F" box="[177,1436,839,863]" gridrow="12" pageId="7" pageNumber="8" rowspan-2="1">
<th id="58626925FFD40065AABDFCAD6D7BFC9F" box="[177,359,839,863]" gridcol="0" gridrow="12" pageId="7" pageNumber="8">Day 9</th>
<td id="58626925FFD40065AB8DFCAD6E38FC9F" box="[385,548,839,863]" gridcol="1" gridrow="12" pageId="7" pageNumber="8">0</td>
<td id="58626925FFD40065A949FCAD6FF4FC9F" box="[837,1000,839,863]" gridcol="3" gridrow="12" pageId="7" pageNumber="8">0.56 ± 0.23</td>
<td id="58626925FFD40065AE0FFCAD68ECFC9F" box="[1027,1264,839,863]" gridcol="4" gridrow="12" pageId="7" pageNumber="8">&lt;0.05</td>
<td id="58626925FFD40065AF0AFCAD6980FC9F" box="[1286,1436,839,863]" gridcol="5" gridrow="12" pageId="7" pageNumber="8">
<geoCoordinate id="C0B764DCFFD4FF9DAF35FCAD6950FC9F" box="[1337,1356,839,863]" degrees="0.05" direction="north" orientation="latitude" pageId="7" pageNumber="8" precision="555" value="0.05">N</geoCoordinate>
.A.
</td>
</tr>
<tr id="1BB30059FFD40065AABDFC856980FC47" box="[177,1436,879,903]" gridrow="13" pageId="7" pageNumber="8" rowspan-1="1" rowspan-2="1">
<th id="58626925FFD40065AABDFC856D7BFC47" box="[177,359,879,903]" gridcol="0" gridrow="13" pageId="7" pageNumber="8">Day 10</th>
<td id="58626925FFD40065A949FC856FF4FC47" box="[837,1000,879,903]" gridcol="3" gridrow="13" pageId="7" pageNumber="8">0.07 ± 0.04</td>
<td id="58626925FFD40065AE0FFC8568ECFC47" box="[1027,1264,879,903]" gridcol="4" gridrow="13" pageId="7" pageNumber="8">&gt;0.05</td>
<td id="58626925FFD40065AF0AFC856980FC47" box="[1286,1436,879,903]" gridcol="5" gridrow="13" pageId="7" pageNumber="8">
<geoCoordinate id="C0B764DCFFD4FF9DAF35FC856950FC47" box="[1337,1356,879,903]" degrees="0.05" direction="north" orientation="latitude" pageId="7" pageNumber="8" precision="555" value="0.05">N</geoCoordinate>
.A.
</td>
</tr>
<tr id="1BB30059FFD40065AABDFC7C6980FC6E" box="[177,1436,918,942]" gridrow="14" pageId="7" pageNumber="8" rowspan-1="1" rowspan-2="1">
<th id="58626925FFD40065AABDFC7C6D7BFC6E" box="[177,359,918,942]" gridcol="0" gridrow="14" pageId="7" pageNumber="8">Day 11</th>
<td id="58626925FFD40065A949FC7C6FF4FC6E" box="[837,1000,918,942]" gridcol="3" gridrow="14" pageId="7" pageNumber="8">0.03 ± 0.03</td>
<td id="58626925FFD40065AE0FFC7C68ECFC6E" box="[1027,1264,918,942]" gridcol="4" gridrow="14" pageId="7" pageNumber="8">&gt;0.05</td>
<td id="58626925FFD40065AF0AFC7C6980FC6E" box="[1286,1436,918,942]" gridcol="5" gridrow="14" pageId="7" pageNumber="8">
<geoCoordinate id="C0B764DCFFD4FF9DAF35FC7C6950FC6E" box="[1337,1356,918,942]" degrees="0.05" direction="north" orientation="latitude" pageId="7" pageNumber="8" precision="555" value="0.05">N</geoCoordinate>
.A.
</td>
</tr>
<tr id="1BB30059FFD40065AABDFC546980FC16" box="[177,1436,958,982]" gridrow="15" pageId="7" pageNumber="8" rowspan-1="1" rowspan-2="1" rowspan-4="1" rowspan-5="1">
<th id="58626925FFD40065AABDFC546D7BFC16" box="[177,359,958,982]" gridcol="0" gridrow="15" pageId="7" pageNumber="8">Day 12</th>
<td id="58626925FFD40065A949FC546FF4FC16" box="[837,1000,958,982]" gridcol="3" gridrow="15" pageId="7" pageNumber="8">0</td>
</tr>
<tr id="1BB30059FFD40065AABDFC066980FBC5" box="[177,1436,1004,1029]" gridrow="16" pageId="7" pageNumber="8" rowspan-2="1" rowspan-4="1" rowspan-5="1">
<th id="58626925FFD40065AABDFC066D7BFBC5" box="[177,359,1004,1029]" gridcol="0" gridrow="16" pageId="7" pageNumber="8">
<emphasis id="97F7DE09FFD4FF9DAABDFC076CA2FBC5" box="[177,190,1005,1029]" italics="true" pageId="7" pageNumber="8">n</emphasis>
<superScript id="52F6AF53FFD4FF9DAAB2FC066CDAFC3A" attach="left" box="[190,198,1004,1018]" fontSize="6" pageId="7" pageNumber="8">a</superScript>
</th>
<td id="58626925FFD40065AB8DFC066E38FBC5" box="[385,548,1004,1029]" gridcol="1" gridrow="16" pageId="7" pageNumber="8">40</td>
<td id="58626925FFD40065A949FC066FF4FBC5" box="[837,1000,1004,1029]" gridcol="3" gridrow="16" pageId="7" pageNumber="8">41</td>
</tr>
<tr id="1BB30059FFD40065AABDFBF66980FB92" box="[177,1436,1052,1106]" gridrow="17" pageId="7" pageNumber="8" rowspan-2="1" rowspan-4="1">
<th id="58626925FFD40065AABDFBF66D7BFB92" box="[177,359,1052,1106]" gridcol="0" gridrow="17" pageId="7" pageNumber="8">
Average brood size per 
<superScript id="52F6AF53FFD4FF9DAB00FBD06D4EFB87" attach="left" box="[268,338,1081,1106]" fontSize="6" pageId="7" pageNumber="8">wormb</superScript>
</th>
<td id="58626925FFD40065AB8DFBF66E38FB92" box="[385,548,1052,1106]" gridcol="1" gridrow="17" pageId="7" pageNumber="8">266.28 ± 6.42</td>
<td id="58626925FFD40065A949FBF66FF4FB92" box="[837,1000,1052,1106]" gridcol="3" gridrow="17" pageId="7" pageNumber="8">137.63 ± 5.74</td>
<td id="58626925FFD40065AF0AFBF66980FB92" box="[1286,1436,1052,1106]" gridcol="5" gridrow="17" pageId="7" pageNumber="8">&lt;0.001</td>
</tr>
<tr id="1BB30059FFD40065AABDFB8A6980FB56" box="[177,1436,1120,1174]" gridrow="18" pageId="7" pageNumber="8" rowspan-2="1" rowspan-4="1">
<th id="58626925FFD40065AABDFB8A6D7BFB56" box="[177,359,1120,1174]" gridcol="0" gridrow="18" pageId="7" pageNumber="8">
RS (day) 
<superScript id="52F6AF53FFD4FF9DAB00FB8A6D08FBAE" attach="left" box="[268,276,1120,1134]" fontSize="6" pageId="7" pageNumber="8">c</superScript>
</th>
<td id="58626925FFD40065AB8DFB8A6E38FB56" box="[385,548,1120,1174]" gridcol="1" gridrow="18" pageId="7" pageNumber="8">4.08 ± 0.16</td>
<td id="58626925FFD40065A949FB8A6FF4FB56" box="[837,1000,1120,1174]" gridcol="3" gridrow="18" pageId="7" pageNumber="8">7.29 ± 0.25</td>
<td id="58626925FFD40065AF0AFB8A6980FB56" box="[1286,1436,1120,1174]" gridcol="5" gridrow="18" pageId="7" pageNumber="8">&lt;0.001 (log-rank test)</td>
</tr>
<tr id="1BB30059FFD40065AABDFB4C6980FB7E" box="[177,1436,1190,1214]" gridrow="19" pageId="7" pageNumber="8">
<th id="58626925FFD40065AABDFB4C6980FB7E" box="[177,1436,1190,1214]" colspan="6" colspanRight="5" gridcol="0" gridrow="19" pageId="7" pageNumber="8">
<taxonomicName id="62837998FFD4FF9DAABDFB4C6D30FB7E" box="[177,300,1190,1214]" pageId="7" pageNumber="8">
<emphasis id="97F7DE09FFD4FF9DAABDFB4C6D30FB7E" box="[177,300,1190,1214]" italics="true" pageId="7" pageNumber="8">C. briggsae</emphasis>
</taxonomicName>
</th>
</tr>
<tr id="1BB30059FFD40065AABDFB276980FB25" box="[177,1436,1229,1253]" gridrow="20" pageId="7" pageNumber="8">
<th id="58626925FFD40065AABDFB276D7BFB25" box="[177,359,1229,1253]" gridcol="0" gridrow="20" pageId="7" pageNumber="8">Day 1</th>
<td id="58626925FFD40065AB8DFB276E38FB25" box="[385,548,1229,1253]" gridcol="1" gridrow="20" pageId="7" pageNumber="8">11.59 ± 1.33</td>
<td id="58626925FFD40065A84CFB276F30FB25" box="[576,812,1229,1253]" gridcol="2" gridrow="20" pageId="7" pageNumber="8">&lt;0.001</td>
<td id="58626925FFD40065A949FB276FF4FB25" box="[837,1000,1229,1253]" gridcol="3" gridrow="20" pageId="7" pageNumber="8">2.11 ± 0.34</td>
<td id="58626925FFD40065AE0FFB2768ECFB25" box="[1027,1264,1229,1253]" gridcol="4" gridrow="20" pageId="7" pageNumber="8">&lt;0.001</td>
<td id="58626925FFD40065AF0AFB276980FB25" box="[1286,1436,1229,1253]" gridcol="5" gridrow="20" pageId="7" pageNumber="8">&lt;0.001</td>
</tr>
<tr id="1BB30059FFD40065AABDFB1F6980FACD" box="[177,1436,1269,1293]" gridrow="21" pageId="7" pageNumber="8">
<th id="58626925FFD40065AABDFB1F6D7BFACD" box="[177,359,1269,1293]" gridcol="0" gridrow="21" pageId="7" pageNumber="8">Day 2</th>
<td id="58626925FFD40065AB8DFB1F6E38FACD" box="[385,548,1269,1293]" gridcol="1" gridrow="21" pageId="7" pageNumber="8">18.21 ± 1.89</td>
<td id="58626925FFD40065A84CFB1F6F30FACD" box="[576,812,1269,1293]" gridcol="2" gridrow="21" pageId="7" pageNumber="8">&lt;0.001</td>
<td id="58626925FFD40065A949FB1F6FF4FACD" box="[837,1000,1269,1293]" gridcol="3" gridrow="21" pageId="7" pageNumber="8">3.80 ± 0.57</td>
<td id="58626925FFD40065AE0FFB1F68ECFACD" box="[1027,1264,1269,1293]" gridcol="4" gridrow="21" pageId="7" pageNumber="8">&lt;0.001</td>
<td id="58626925FFD40065AF0AFB1F6980FACD" box="[1286,1436,1269,1293]" gridcol="5" gridrow="21" pageId="7" pageNumber="8">&lt;0.001</td>
</tr>
<tr id="1BB30059FFD40065AABDFAF66980FAF4" box="[177,1436,1308,1332]" gridrow="22" pageId="7" pageNumber="8">
<th id="58626925FFD40065AABDFAF66D7BFAF4" box="[177,359,1308,1332]" gridcol="0" gridrow="22" pageId="7" pageNumber="8">Day 3</th>
<td id="58626925FFD40065AB8DFAF66E38FAF4" box="[385,548,1308,1332]" gridcol="1" gridrow="22" pageId="7" pageNumber="8">22.56 ± 3.46</td>
<td id="58626925FFD40065A84CFAF66F30FAF4" box="[576,812,1308,1332]" gridcol="2" gridrow="22" pageId="7" pageNumber="8">&lt;0.001</td>
<td id="58626925FFD40065A949FAF66FF4FAF4" box="[837,1000,1308,1332]" gridcol="3" gridrow="22" pageId="7" pageNumber="8">3.17 ± 0.56</td>
<td id="58626925FFD40065AE0FFAF668ECFAF4" box="[1027,1264,1308,1332]" gridcol="4" gridrow="22" pageId="7" pageNumber="8">&lt;0.001</td>
<td id="58626925FFD40065AF0AFAF66980FAF4" box="[1286,1436,1308,1332]" gridcol="5" gridrow="22" pageId="7" pageNumber="8">&lt;0.001</td>
</tr>
<tr id="1BB30059FFD40065AABDFAAE6980FA9C" box="[177,1436,1348,1372]" gridrow="23" pageId="7" pageNumber="8">
<th id="58626925FFD40065AABDFAAE6D7BFA9C" box="[177,359,1348,1372]" gridcol="0" gridrow="23" pageId="7" pageNumber="8">Day 4</th>
<td id="58626925FFD40065AB8DFAAE6E38FA9C" box="[385,548,1348,1372]" gridcol="1" gridrow="23" pageId="7" pageNumber="8">12.00 ± 2.52</td>
<td id="58626925FFD40065A84CFAAE6F30FA9C" box="[576,812,1348,1372]" gridcol="2" gridrow="23" pageId="7" pageNumber="8">&lt;0.001</td>
<td id="58626925FFD40065A949FAAE6FF4FA9C" box="[837,1000,1348,1372]" gridcol="3" gridrow="23" pageId="7" pageNumber="8">2.03 ± 0.50</td>
<td id="58626925FFD40065AE0FFAAE68ECFA9C" box="[1027,1264,1348,1372]" gridcol="4" gridrow="23" pageId="7" pageNumber="8">&lt;0.001</td>
<td id="58626925FFD40065AF0AFAAE6980FA9C" box="[1286,1436,1348,1372]" gridcol="5" gridrow="23" pageId="7" pageNumber="8">&lt;0.001</td>
</tr>
<tr id="1BB30059FFD40065AABDFA816980FA43" box="[177,1436,1387,1411]" gridrow="24" pageId="7" pageNumber="8">
<th id="58626925FFD40065AABDFA816D7BFA43" box="[177,359,1387,1411]" gridcol="0" gridrow="24" pageId="7" pageNumber="8">Day 5</th>
<td id="58626925FFD40065AB8DFA816E38FA43" box="[385,548,1387,1411]" gridcol="1" gridrow="24" pageId="7" pageNumber="8">0.88 ± 0.28</td>
<td id="58626925FFD40065A84CFA816F30FA43" box="[576,812,1387,1411]" gridcol="2" gridrow="24" pageId="7" pageNumber="8">&lt;0.01</td>
<td id="58626925FFD40065A949FA816FF4FA43" box="[837,1000,1387,1411]" gridcol="3" gridrow="24" pageId="7" pageNumber="8">2.30 ± 0.50</td>
<td id="58626925FFD40065AE0FFA8168ECFA43" box="[1027,1264,1387,1411]" gridcol="4" gridrow="24" pageId="7" pageNumber="8">
&lt;
<geoCoordinate id="C0B764DCFFD4FF9DAE5AFA81688AFA43" box="[1110,1174,1387,1411]" degrees="0.001" direction="north" orientation="latitude" pageId="7" pageNumber="8" precision="55" value="0.001">0.001</geoCoordinate>
</td>
<td id="58626925FFD40065AF0AFA816980FA43" box="[1286,1436,1387,1411]" gridcol="5" gridrow="24" pageId="7" pageNumber="8">
<geoCoordinate id="C0B764DCFFD4FF9DAF39FA81699EFA43" box="[1333,1410,1387,1411]" degrees="0.0109" direction="east" orientation="longitude" pageId="7" pageNumber="8" precision="5" value="0.0109">0.0109</geoCoordinate>
</td>
</tr>
<tr id="1BB30059FFD40065AABDFA786980FA6A" box="[177,1436,1426,1450]" gridrow="25" pageId="7" pageNumber="8">
<th id="58626925FFD40065AABDFA786D7BFA6A" box="[177,359,1426,1450]" gridcol="0" gridrow="25" pageId="7" pageNumber="8">Day 6</th>
<td id="58626925FFD40065AB8DFA786E38FA6A" box="[385,548,1426,1450]" gridcol="1" gridrow="25" pageId="7" pageNumber="8">0.16 ± 0.10</td>
<td id="58626925FFD40065A84CFA786F30FA6A" box="[576,812,1426,1450]" gridcol="2" gridrow="25" pageId="7" pageNumber="8">&gt;0.05</td>
<td id="58626925FFD40065A949FA786FF4FA6A" box="[837,1000,1426,1450]" gridcol="3" gridrow="25" pageId="7" pageNumber="8">1.69 ± 0.44</td>
<td id="58626925FFD40065AE0FFA7868ECFA6A" box="[1027,1264,1426,1450]" gridcol="4" gridrow="25" pageId="7" pageNumber="8">&lt;0.001</td>
<td id="58626925FFD40065AF0AFA786980FA6A" box="[1286,1436,1426,1450]" gridcol="5" gridrow="25" pageId="7" pageNumber="8">&lt;0.01</td>
</tr>
<tr id="1BB30059FFD40065AABDFA506980FA12" box="[177,1436,1466,1490]" gridrow="26" pageId="7" pageNumber="8" rowspan-2="1">
<th id="58626925FFD40065AABDFA506D7BFA12" box="[177,359,1466,1490]" gridcol="0" gridrow="26" pageId="7" pageNumber="8">Day 7</th>
<td id="58626925FFD40065AB8DFA506E38FA12" box="[385,548,1466,1490]" gridcol="1" gridrow="26" pageId="7" pageNumber="8">0</td>
<td id="58626925FFD40065A949FA506FF4FA12" box="[837,1000,1466,1490]" gridcol="3" gridrow="26" pageId="7" pageNumber="8">0.50 ± 0.17</td>
<td id="58626925FFD40065AE0FFA5068ECFA12" box="[1027,1264,1466,1490]" gridcol="4" gridrow="26" pageId="7" pageNumber="8">&lt;0.001</td>
<td id="58626925FFD40065AF0AFA506980FA12" box="[1286,1436,1466,1490]" gridcol="5" gridrow="26" pageId="7" pageNumber="8">
<geoCoordinate id="C0B764DCFFD4FF9DAF35FA506950FA12" box="[1337,1356,1466,1490]" degrees="0.001" direction="north" orientation="latitude" pageId="7" pageNumber="8" precision="55" value="0.001">N</geoCoordinate>
.A.
</td>
</tr>
<tr id="1BB30059FFD40065AABDFA0B6980FA39" box="[177,1436,1505,1529]" gridrow="27" pageId="7" pageNumber="8" rowspan-1="1" rowspan-2="1">
<th id="58626925FFD40065AABDFA0B6D7BFA39" box="[177,359,1505,1529]" gridcol="0" gridrow="27" pageId="7" pageNumber="8">Day 8</th>
<td id="58626925FFD40065A949FA0B6FF4FA39" box="[837,1000,1505,1529]" gridcol="3" gridrow="27" pageId="7" pageNumber="8">0.22 ± 0.07</td>
<td id="58626925FFD40065AE0FFA0B68ECFA39" box="[1027,1264,1505,1529]" gridcol="4" gridrow="27" pageId="7" pageNumber="8">&lt;0.01</td>
<td id="58626925FFD40065AF0AFA0B6980FA39" box="[1286,1436,1505,1529]" gridcol="5" gridrow="27" pageId="7" pageNumber="8">
<geoCoordinate id="C0B764DCFFD4FF9DAF35FA0B6950FA39" box="[1337,1356,1505,1529]" degrees="0.01" direction="north" orientation="latitude" pageId="7" pageNumber="8" precision="555" value="0.01">N</geoCoordinate>
.A.
</td>
</tr>
<tr id="1BB30059FFD40065AABDF9E36980F9E1" box="[177,1436,1545,1569]" gridrow="28" pageId="7" pageNumber="8" rowspan-1="1" rowspan-2="1">
<th id="58626925FFD40065AABDF9E36D7BF9E1" box="[177,359,1545,1569]" gridcol="0" gridrow="28" pageId="7" pageNumber="8">Day 9</th>
<td id="58626925FFD40065A949F9E36FF4F9E1" box="[837,1000,1545,1569]" gridcol="3" gridrow="28" pageId="7" pageNumber="8">0.08 ± 0.08</td>
<td id="58626925FFD40065AE0FF9E368ECF9E1" box="[1027,1264,1545,1569]" gridcol="4" gridrow="28" pageId="7" pageNumber="8">&gt;0.05</td>
<td id="58626925FFD40065AF0AF9E36980F9E1" box="[1286,1436,1545,1569]" gridcol="5" gridrow="28" pageId="7" pageNumber="8">
<geoCoordinate id="C0B764DCFFD4FF9DAF35F9E36950F9E1" box="[1337,1356,1545,1569]" degrees="0.05" direction="north" orientation="latitude" pageId="7" pageNumber="8" precision="555" value="0.05">N</geoCoordinate>
.A.
</td>
</tr>
<tr id="1BB30059FFD40065AABDF9DA6980F988" box="[177,1436,1584,1608]" gridrow="29" pageId="7" pageNumber="8" rowspan-1="1" rowspan-2="1">
<th id="58626925FFD40065AABDF9DA6D7BF988" box="[177,359,1584,1608]" gridcol="0" gridrow="29" pageId="7" pageNumber="8">Day 10</th>
<td id="58626925FFD40065A949F9DA6FF4F988" box="[837,1000,1584,1608]" gridcol="3" gridrow="29" pageId="7" pageNumber="8">0.08 ± 0.08</td>
<td id="58626925FFD40065AE0FF9DA68ECF988" box="[1027,1264,1584,1608]" gridcol="4" gridrow="29" pageId="7" pageNumber="8">&gt;0.05</td>
<td id="58626925FFD40065AF0AF9DA6980F988" box="[1286,1436,1584,1608]" gridcol="5" gridrow="29" pageId="7" pageNumber="8">
<geoCoordinate id="C0B764DCFFD4FF9DAF35F9DA6950F988" box="[1337,1356,1584,1608]" degrees="0.05" direction="north" orientation="latitude" pageId="7" pageNumber="8" precision="555" value="0.05">N</geoCoordinate>
.A.
</td>
</tr>
<tr id="1BB30059FFD40065AABDF9BD6980F9AF" box="[177,1436,1623,1647]" gridrow="30" pageId="7" pageNumber="8" rowspan-1="1" rowspan-2="1" rowspan-4="1" rowspan-5="1">
<th id="58626925FFD40065AABDF9BD6D7BF9AF" box="[177,359,1623,1647]" gridcol="0" gridrow="30" pageId="7" pageNumber="8">Day 11</th>
<td id="58626925FFD40065A949F9BD6FF4F9AF" box="[837,1000,1623,1647]" gridcol="3" gridrow="30" pageId="7" pageNumber="8">0</td>
</tr>
<tr id="1BB30059FFD40065AABDF9946980F957" box="[177,1436,1662,1687]" gridrow="31" pageId="7" pageNumber="8" rowspan-2="1" rowspan-4="1" rowspan-5="1">
<th id="58626925FFD40065AABDF9946D7BF957" box="[177,359,1662,1687]" gridcol="0" gridrow="31" pageId="7" pageNumber="8">
<emphasis id="97F7DE09FFD4FF9DAABDF9956CDAF94C" box="[177,198,1662,1687]" italics="true" pageId="7" pageNumber="8">
<superScript id="52F6AF53FFD4FF9DAABDF9956CDAF94C" attach="left" box="[177,198,1662,1687]" fontSize="6" pageId="7" pageNumber="8">na</superScript>
</emphasis>
</th>
<td id="58626925FFD40065AB8DF9946E38F957" box="[385,548,1662,1687]" gridcol="1" gridrow="31" pageId="7" pageNumber="8">32</td>
<td id="58626925FFD40065A949F9946FF4F957" box="[837,1000,1662,1687]" gridcol="3" gridrow="31" pageId="7" pageNumber="8">36</td>
</tr>
<tr id="1BB30059FFD40065AABDF94C6980F91C" box="[177,1436,1702,1756]" gridrow="32" pageId="7" pageNumber="8" rowspan-2="1" rowspan-4="1">
<th id="58626925FFD40065AABDF94C6D7BF91C" box="[177,359,1702,1756]" gridcol="0" gridrow="32" pageId="7" pageNumber="8">
Average brood size per 
<superScript id="52F6AF53FFD4FF9DAB00F92E6D4EF911" attach="left" box="[268,338,1731,1756]" fontSize="6" pageId="7" pageNumber="8">wormb</superScript>
</th>
<td id="58626925FFD40065AB8DF94C6E38F91C" box="[385,548,1702,1756]" gridcol="1" gridrow="32" pageId="7" pageNumber="8">65.40 ± 7.09</td>
<td id="58626925FFD40065A949F94C6FF4F91C" box="[837,1000,1702,1756]" gridcol="3" gridrow="32" pageId="7" pageNumber="8">16.00 ± 1.88</td>
<td id="58626925FFD40065AF0AF94C6980F91C" box="[1286,1436,1702,1756]" gridcol="5" gridrow="32" pageId="7" pageNumber="8">&lt;0.001</td>
</tr>
<tr id="1BB30059FFD40065AABDF9006980F8E0" box="[177,1436,1770,1824]" gridrow="33" pageId="7" pageNumber="8" rowspan-2="1" rowspan-4="1">
<th id="58626925FFD40065AABDF9006D7BF8E0" box="[177,359,1770,1824]" gridcol="0" gridrow="33" pageId="7" pageNumber="8">
RS (day) 
<superScript id="52F6AF53FFD4FF9DAB00F9006D08F938" attach="left" box="[268,276,1770,1784]" fontSize="6" pageId="7" pageNumber="8">c</superScript>
</th>
<td id="58626925FFD40065AB8DF9006E38F8E0" box="[385,548,1770,1824]" gridcol="1" gridrow="33" pageId="7" pageNumber="8">3.97 ± 0.25</td>
<td id="58626925FFD40065A949F9006FF4F8E0" box="[837,1000,1770,1824]" gridcol="3" gridrow="33" pageId="7" pageNumber="8">5.61 ± 0.39</td>
<td id="58626925FFD40065AF0AF9006980F8E0" box="[1286,1436,1770,1824]" gridcol="5" gridrow="33" pageId="7" pageNumber="8">&lt;0.001 (log-rank test)</td>
</tr>
<tr id="1BB30059FFD40065AABDF8DA6980F888" box="[177,1436,1840,1864]" gridrow="34" pageId="7" pageNumber="8">
<th id="58626925FFD40065AABDF8DA6980F888" box="[177,1436,1840,1864]" colspan="6" colspanRight="5" gridcol="0" gridrow="34" pageId="7" pageNumber="8">
<taxonomicName id="62837998FFD4FF9DAABDF8DA6D32F888" box="[177,302,1840,1864]" pageId="7" pageNumber="8">
<emphasis id="97F7DE09FFD4FF9DAABDF8DA6D32F888" box="[177,302,1840,1864]" italics="true" pageId="7" pageNumber="8">C. tropicalis</emphasis>
</taxonomicName>
</th>
</tr>
<tr id="1BB30059FFD40065AABDF8BD6980F8AF" box="[177,1436,1879,1903]" gridrow="35" pageId="7" pageNumber="8">
<th id="58626925FFD40065AABDF8BD6D7BF8AF" box="[177,359,1879,1903]" gridcol="0" gridrow="35" pageId="7" pageNumber="8">Day 1</th>
<td id="58626925FFD40065AB8DF8BD6E38F8AF" box="[385,548,1879,1903]" gridcol="1" gridrow="35" pageId="7" pageNumber="8">4.37 ± 0.78</td>
<td id="58626925FFD40065A84CF8BD6F30F8AF" box="[576,812,1879,1903]" gridcol="2" gridrow="35" pageId="7" pageNumber="8">&lt;0.001</td>
<td id="58626925FFD40065A949F8BD6FF4F8AF" box="[837,1000,1879,1903]" gridcol="3" gridrow="35" pageId="7" pageNumber="8">4.75 ± 0.65</td>
<td id="58626925FFD40065AE0FF8BD68ECF8AF" box="[1027,1264,1879,1903]" gridcol="4" gridrow="35" pageId="7" pageNumber="8">&lt;0.001</td>
<td id="58626925FFD40065AF0AF8BD6980F8AF" box="[1286,1436,1879,1903]" gridcol="5" gridrow="35" pageId="7" pageNumber="8">1.0</td>
</tr>
</table>
</paragraph>
<subSection id="CC0C1BF0FFD4FF9DAF3DF8686985F856" box="[1329,1433,1922,1942]" pageId="7" pageNumber="8" type="multiple">
<paragraph id="A53C021BFFD4FF9DAF3DF8686985F856" blockId="7.[1329,1433,1922,1940]" box="[1329,1433,1922,1942]" pageId="7" pageNumber="8">
<emphasis id="97F7DE09FFD4FF9DAF3DF8686985F856" box="[1329,1433,1922,1942]" italics="true" pageId="7" pageNumber="8">(Continued)</emphasis>
</paragraph>
</subSection>
<caption id="F1FC5293FFDBFF92AA9BFF576DB7FF1B" box="[151,427,189,219]" isContinuationCaption="true" pageId="8" pageNumber="9" startId="8.[151,230,189,219]" targetBox="[162,1421,259,1797]" targetIsTable="true" targetPageId="8" targetType="table">
<paragraph id="A53C021BFFDBFF92AA9BFF576DB7FF1B" blockId="8.[151,427,189,219]" box="[151,427,189,219]" pageId="8" pageNumber="9">Table 2. Continued</paragraph>
</caption>
<paragraph id="A53C021BFFDBFF92AB7EFEE96991F8C5" pageId="8" pageNumber="9">
<table id="D783F0BBFFDB0065AAAEFEE96991F8C5" box="[162,1421,259,1797]" gridcols="6" gridrows="34" pageId="8" pageNumber="9">
<tr id="1BB30059FFDB0065AAAEFEE96991FEDE" box="[162,1421,259,286]" gridrow="0" pageId="8" pageNumber="9" rowspan-0="1" rowspan-2="1" rowspan-4="1">
<th id="58626925FFDB0065AB7EFEE96E07FEDE" box="[370,539,259,286]" gridcol="1" gridrow="0" pageId="8" pageNumber="9">
<emphasis id="97F7DE09FFDBFF92AB7EFEE96DA0FEDE" bold="true" box="[370,444,259,286]" pageId="8" pageNumber="9">OP50</emphasis>
</th>
<th id="58626925FFDB0065A93AFEE96FC5FEDE" box="[822,985,259,286]" gridcol="3" gridrow="0" pageId="8" pageNumber="9">CFBb37</th>
<th id="58626925FFDB0065AEFBFEE96991FEDE" box="[1271,1421,259,286]" gridcol="5" gridrow="0" pageId="8" pageNumber="9">
<emphasis id="97F7DE09FFDBFF92AEFBFEE96977FEDE" bold="true" box="[1271,1387,259,286]" pageId="8" pageNumber="9">Different</emphasis>
</th>
</tr>
<tr id="1BB30059FFDB0065AAAEFEC16991FE56" box="[162,1421,299,406]" gridrow="1" pageId="8" pageNumber="9">
<th id="58626925FFDB0065AAAEFEC16D44FE56" box="[162,344,299,406]" gridcol="0" gridrow="1" pageId="8" pageNumber="9">Reproductive day number</th>
<td id="58626925FFDB0065AB7EFEC16E07FE56" box="[370,539,299,406]" gridcol="1" gridrow="1" pageId="8" pageNumber="9">Eggs laid per day</td>
<td id="58626925FFDB0065A83CFEC16F01FE56" box="[560,797,299,406]" gridcol="2" gridrow="1" pageId="8" pageNumber="9">
<emphasis id="97F7DE09FFDBFF92A83CFEA76F01FEA8" bold="true" box="[560,797,333,360]" pageId="8" pageNumber="9">Greater than zero</emphasis>
<emphasis id="97F7DE09FFDBFF92A83CFE9F6E58FE50" bold="true" box="[560,580,373,400]" pageId="8" pageNumber="9">P</emphasis>
</td>
<td id="58626925FFDB0065A93AFEC16FC5FE56" box="[822,985,299,406]" gridcol="3" gridrow="1" pageId="8" pageNumber="9">Eggs laid per day</td>
<td id="58626925FFDB0065A9F8FEC168FDFE56" box="[1012,1249,299,406]" gridcol="4" gridrow="1" pageId="8" pageNumber="9">Greater than zero P</td>
<td id="58626925FFDB0065AEFBFEC16991FE56" box="[1271,1421,299,406]" gridcol="5" gridrow="1" pageId="8" pageNumber="9">between the strains P (paired</td>
</tr>
<tr id="1BB30059FFDB0065AAAEFE776991FE7E" box="[162,1421,413,446]" gridrow="2" pageId="8" pageNumber="9" rowspan-0="1">
<td id="58626925FFDB0065AB7EFE776E07FE7E" box="[370,539,413,446]" gridcol="1" gridrow="2" pageId="8" pageNumber="9">(mean ± SE)</td>
<td id="58626925FFDB0065A83CFE776F01FE7E" box="[560,797,413,446]" gridcol="2" gridrow="2" pageId="8" pageNumber="9">
<emphasis id="97F7DE09FFDBFF92A83CFE776F00FE78" bold="true" box="[560,796,413,440]" pageId="8" pageNumber="9">(one-sample t- test)</emphasis>
</td>
<td id="58626925FFDB0065A93AFE776FC5FE7E" box="[822,985,413,446]" gridcol="3" gridrow="2" pageId="8" pageNumber="9">(mean ± SE)</td>
<td id="58626925FFDB0065A9F8FE7768FDFE7E" box="[1012,1249,413,446]" gridcol="4" gridrow="2" pageId="8" pageNumber="9">(one-sample t- test)</td>
<td id="58626925FFDB0065AEFBFE776991FE7E" box="[1271,1421,413,446]" gridcol="5" gridrow="2" pageId="8" pageNumber="9">
<emphasis id="97F7DE09FFDBFF92AEFBFE49695BFE7E" bold="true" box="[1271,1351,419,446]" pageId="8" pageNumber="9">t- test)</emphasis>
</td>
</tr>
<tr id="1BB30059FFDB0065AAAEFE336991FE31" box="[162,1421,473,497]" gridrow="3" pageId="8" pageNumber="9">
<th id="58626925FFDB0065AAAEFE336D44FE31" box="[162,344,473,497]" gridcol="0" gridrow="3" pageId="8" pageNumber="9">Day 2</th>
<td id="58626925FFDB0065AB7EFE336E07FE31" box="[370,539,473,497]" gridcol="1" gridrow="3" pageId="8" pageNumber="9">4.81 ± 1.37</td>
<td id="58626925FFDB0065A83CFE336F01FE31" box="[560,797,473,497]" gridcol="2" gridrow="3" pageId="8" pageNumber="9">&lt;0.001</td>
<td id="58626925FFDB0065A93AFE336FC5FE31" box="[822,985,473,497]" gridcol="3" gridrow="3" pageId="8" pageNumber="9">4.79 ± 0.92</td>
<td id="58626925FFDB0065A9F8FE3368FDFE31" box="[1012,1249,473,497]" gridcol="4" gridrow="3" pageId="8" pageNumber="9">
&lt;
<geoCoordinate id="C0B764DCFFDBFF92AE4BFE33689BFE31" box="[1095,1159,473,497]" degrees="0.001" direction="north" orientation="latitude" pageId="8" pageNumber="9" precision="55" value="0.001">0.001</geoCoordinate>
</td>
<td id="58626925FFDB0065AEFBFE336991FE31" box="[1271,1421,473,497]" gridcol="5" gridrow="3" pageId="8" pageNumber="9">
<geoCoordinate id="C0B764DCFFDBFF92AF2AFE33696FFE31" box="[1318,1395,473,497]" degrees="0.8652" direction="east" orientation="longitude" pageId="8" pageNumber="9" precision="5" value="0.8652">0.8652</geoCoordinate>
</td>
</tr>
<tr id="1BB30059FFDB0065AAAEFDEB6991FDD9" box="[162,1421,513,537]" gridrow="4" pageId="8" pageNumber="9">
<th id="58626925FFDB0065AAAEFDEB6D44FDD9" box="[162,344,513,537]" gridcol="0" gridrow="4" pageId="8" pageNumber="9">Day 3</th>
<td id="58626925FFDB0065AB7EFDEB6E07FDD9" box="[370,539,513,537]" gridcol="1" gridrow="4" pageId="8" pageNumber="9">1.22 ± 0.39</td>
<td id="58626925FFDB0065A83CFDEB6F01FDD9" box="[560,797,513,537]" gridcol="2" gridrow="4" pageId="8" pageNumber="9">&lt;0.01</td>
<td id="58626925FFDB0065A93AFDEB6FC5FDD9" box="[822,985,513,537]" gridcol="3" gridrow="4" pageId="8" pageNumber="9">7.20 ± 1.71</td>
<td id="58626925FFDB0065A9F8FDEB68FDFDD9" box="[1012,1249,513,537]" gridcol="4" gridrow="4" pageId="8" pageNumber="9">&lt;0.001</td>
<td id="58626925FFDB0065AEFBFDEB6991FDD9" box="[1271,1421,513,537]" gridcol="5" gridrow="4" pageId="8" pageNumber="9">&lt;0.01</td>
</tr>
<tr id="1BB30059FFDB0065AAAEFDC26991FD80" box="[162,1421,552,576]" gridrow="5" pageId="8" pageNumber="9">
<th id="58626925FFDB0065AAAEFDC26D44FD80" box="[162,344,552,576]" gridcol="0" gridrow="5" pageId="8" pageNumber="9">Day 4</th>
<td id="58626925FFDB0065AB7EFDC26E07FD80" box="[370,539,552,576]" gridcol="1" gridrow="5" pageId="8" pageNumber="9">0.37 ± 0.24</td>
<td id="58626925FFDB0065A83CFDC26F01FD80" box="[560,797,552,576]" gridcol="2" gridrow="5" pageId="8" pageNumber="9">&gt;0.05</td>
<td id="58626925FFDB0065A93AFDC26FC5FD80" box="[822,985,552,576]" gridcol="3" gridrow="5" pageId="8" pageNumber="9">3.88 ± 1.05</td>
<td id="58626925FFDB0065A9F8FDC268FDFD80" box="[1012,1249,552,576]" gridcol="4" gridrow="5" pageId="8" pageNumber="9">&lt;0.01</td>
<td id="58626925FFDB0065AEFBFDC26991FD80" box="[1271,1421,552,576]" gridcol="5" gridrow="5" pageId="8" pageNumber="9">&lt;0.01</td>
</tr>
<tr id="1BB30059FFDB0065AAAEFDBA6991FDA8" box="[162,1421,592,616]" gridrow="6" pageId="8" pageNumber="9" rowspan-2="1">
<th id="58626925FFDB0065AAAEFDBA6D44FDA8" box="[162,344,592,616]" gridcol="0" gridrow="6" pageId="8" pageNumber="9">Day 5</th>
<td id="58626925FFDB0065AB7EFDBA6E07FDA8" box="[370,539,592,616]" gridcol="1" gridrow="6" pageId="8" pageNumber="9">0</td>
<td id="58626925FFDB0065A93AFDBA6FC5FDA8" box="[822,985,592,616]" gridcol="3" gridrow="6" pageId="8" pageNumber="9">3.29 ± 0.92</td>
<td id="58626925FFDB0065A9F8FDBA68FDFDA8" box="[1012,1249,592,616]" gridcol="4" gridrow="6" pageId="8" pageNumber="9">&lt;0.01</td>
<td id="58626925FFDB0065AEFBFDBA6991FDA8" box="[1271,1421,592,616]" gridcol="5" gridrow="6" pageId="8" pageNumber="9">
<geoCoordinate id="C0B764DCFFDBFF92AF26FDBA6921FDA8" box="[1322,1341,592,616]" degrees="0.01" direction="north" orientation="latitude" pageId="8" pageNumber="9" precision="555" value="0.01">N</geoCoordinate>
.A.
</td>
</tr>
<tr id="1BB30059FFDB0065AAAEFD9D6991FD4F" box="[162,1421,631,655]" gridrow="7" pageId="8" pageNumber="9" rowspan-1="1" rowspan-2="1">
<th id="58626925FFDB0065AAAEFD9D6D44FD4F" box="[162,344,631,655]" gridcol="0" gridrow="7" pageId="8" pageNumber="9">Day 6</th>
<td id="58626925FFDB0065A93AFD9D6FC5FD4F" box="[822,985,631,655]" gridcol="3" gridrow="7" pageId="8" pageNumber="9">0.95 ± 0.29</td>
<td id="58626925FFDB0065A9F8FD9D68FDFD4F" box="[1012,1249,631,655]" gridcol="4" gridrow="7" pageId="8" pageNumber="9">&lt;0.01</td>
<td id="58626925FFDB0065AEFBFD9D6991FD4F" box="[1271,1421,631,655]" gridcol="5" gridrow="7" pageId="8" pageNumber="9">
<geoCoordinate id="C0B764DCFFDBFF92AF26FD9D6921FD4F" box="[1322,1341,631,655]" degrees="0.01" direction="north" orientation="latitude" pageId="8" pageNumber="9" precision="555" value="0.01">N</geoCoordinate>
.A.
</td>
</tr>
<tr id="1BB30059FFDB0065AAAEFD746991FD76" box="[162,1421,670,694]" gridrow="8" pageId="8" pageNumber="9" rowspan-1="1" rowspan-2="1">
<th id="58626925FFDB0065AAAEFD746D44FD76" box="[162,344,670,694]" gridcol="0" gridrow="8" pageId="8" pageNumber="9">Day 7</th>
<td id="58626925FFDB0065A93AFD746FC5FD76" box="[822,985,670,694]" gridcol="3" gridrow="8" pageId="8" pageNumber="9">0.33 ± 0.14</td>
<td id="58626925FFDB0065A9F8FD7468FDFD76" box="[1012,1249,670,694]" gridcol="4" gridrow="8" pageId="8" pageNumber="9">&lt;0.05</td>
<td id="58626925FFDB0065AEFBFD746991FD76" box="[1271,1421,670,694]" gridcol="5" gridrow="8" pageId="8" pageNumber="9">
<geoCoordinate id="C0B764DCFFDBFF92AF26FD746921FD76" box="[1322,1341,670,694]" degrees="0.05" direction="north" orientation="latitude" pageId="8" pageNumber="9" precision="555" value="0.05">N</geoCoordinate>
.A.
</td>
</tr>
<tr id="1BB30059FFDB0065AAAEFD2C6991FD1E" box="[162,1421,710,734]" gridrow="9" pageId="8" pageNumber="9" rowspan-1="1" rowspan-2="1">
<th id="58626925FFDB0065AAAEFD2C6D44FD1E" box="[162,344,710,734]" gridcol="0" gridrow="9" pageId="8" pageNumber="9">Day 8</th>
<td id="58626925FFDB0065A93AFD2C6FC5FD1E" box="[822,985,710,734]" gridcol="3" gridrow="9" pageId="8" pageNumber="9">0.04 ± 0.04</td>
<td id="58626925FFDB0065A9F8FD2C68FDFD1E" box="[1012,1249,710,734]" gridcol="4" gridrow="9" pageId="8" pageNumber="9">&gt;0.05</td>
<td id="58626925FFDB0065AEFBFD2C6991FD1E" box="[1271,1421,710,734]" gridcol="5" gridrow="9" pageId="8" pageNumber="9">
<geoCoordinate id="C0B764DCFFDBFF92AF26FD2C6921FD1E" box="[1322,1341,710,734]" degrees="0.05" direction="north" orientation="latitude" pageId="8" pageNumber="9" precision="555" value="0.05">N</geoCoordinate>
.A.
</td>
</tr>
<tr id="1BB30059FFDB0065AAAEFD076991FCC5" box="[162,1421,749,773]" gridrow="10" pageId="8" pageNumber="9" rowspan-1="1" rowspan-2="1" rowspan-4="1">
<th id="58626925FFDB0065AAAEFD076D44FCC5" box="[162,344,749,773]" gridcol="0" gridrow="10" pageId="8" pageNumber="9">Day 9</th>
<td id="58626925FFDB0065A93AFD076FC5FCC5" box="[822,985,749,773]" gridcol="3" gridrow="10" pageId="8" pageNumber="9">0</td>
<td id="58626925FFDB0065AEFBFD076991FCC5" box="[1271,1421,749,773]" gridcol="5" gridrow="10" pageId="8" pageNumber="9">N.A.</td>
</tr>
<tr id="1BB30059FFDB0065AAAEFCFE6991FCED" box="[162,1421,788,813]" gridrow="11" pageId="8" pageNumber="9" rowspan-2="1" rowspan-4="1" rowspan-5="1">
<th id="58626925FFDB0065AAAEFCFE6D44FCED" box="[162,344,788,813]" gridcol="0" gridrow="11" pageId="8" pageNumber="9">
<emphasis id="97F7DE09FFDBFF92AAAEFCFF6CABFCE2" box="[162,183,788,813]" italics="true" pageId="8" pageNumber="9">
<superScript id="52F6AF53FFDBFF92AAAEFCFF6CABFCE2" attach="left" box="[162,183,788,813]" fontSize="6" pageId="8" pageNumber="9">na</superScript>
</emphasis>
</th>
<td id="58626925FFDB0065AB7EFCFE6E07FCED" box="[370,539,788,813]" gridcol="1" gridrow="11" pageId="8" pageNumber="9">27</td>
<td id="58626925FFDB0065A93AFCFE6FC5FCED" box="[822,985,788,813]" gridcol="3" gridrow="11" pageId="8" pageNumber="9">24</td>
</tr>
<tr id="1BB30059FFDB0065AAAEFCD66991FCB1" box="[162,1421,828,881]" gridrow="12" pageId="8" pageNumber="9" rowspan-2="1" rowspan-4="1">
<th id="58626925FFDB0065AAAEFCD66D44FCB1" box="[162,344,828,881]" gridcol="0" gridrow="12" pageId="8" pageNumber="9">
Average brood size per 
<superScript id="52F6AF53FFDBFF92AAF1FCB36D5FFCA7" attach="left" box="[253,323,857,881]" fontSize="6" pageId="8" pageNumber="9">wormb</superScript>
</th>
<td id="58626925FFDB0065AB7EFCD66E07FCB1" box="[370,539,828,881]" gridcol="1" gridrow="12" pageId="8" pageNumber="9">10.78 ± 1.90</td>
<td id="58626925FFDB0065A93AFCD66FC5FCB1" box="[822,985,828,881]" gridcol="3" gridrow="12" pageId="8" pageNumber="9">25.25 ± 3.59</td>
<td id="58626925FFDB0065AEFBFCD66991FCB1" box="[1271,1421,828,881]" gridcol="5" gridrow="12" pageId="8" pageNumber="9">&lt;0.01</td>
</tr>
<tr id="1BB30059FFDB0065AAAEFC6A6991FC76" box="[162,1421,896,950]" gridrow="13" pageId="8" pageNumber="9" rowspan-2="1" rowspan-4="1">
<th id="58626925FFDB0065AAAEFC6A6D44FC76" box="[162,344,896,950]" gridcol="0" gridrow="13" pageId="8" pageNumber="9">
RS (day) 
<superScript id="52F6AF53FFDBFF92AAF1FC6A6D19FC4E" attach="left" box="[253,261,896,910]" fontSize="6" pageId="8" pageNumber="9">c</superScript>
</th>
<td id="58626925FFDB0065AB7EFC6A6E07FC76" box="[370,539,896,950]" gridcol="1" gridrow="13" pageId="8" pageNumber="9">2.37 ± 0.20</td>
<td id="58626925FFDB0065A93AFC6A6FC5FC76" box="[822,985,896,950]" gridcol="3" gridrow="13" pageId="8" pageNumber="9">4.96 ± 0.36</td>
<td id="58626925FFDB0065AEFBFC6A6991FC76" box="[1271,1421,896,950]" gridcol="5" gridrow="13" pageId="8" pageNumber="9">&lt;0.001 (log-rank test)</td>
</tr>
<tr id="1BB30059FFDB0065AAAEFC2C6991FC1E" box="[162,1421,966,990]" gridrow="14" pageId="8" pageNumber="9">
<th id="58626925FFDB0065AAAEFC2C6991FC1E" box="[162,1421,966,990]" colspan="6" colspanRight="5" gridcol="0" gridrow="14" pageId="8" pageNumber="9">
<heading id="FE74B577FFDBFF92AAAEFC2C6D7CFC1E" box="[162,352,966,990]" fontSize="10" level="2" pageId="8" pageNumber="9" reason="3">
<taxonomicName id="62837998FFDBFF92AAAEFC2C6D7CFC1E" box="[162,352,966,990]" class="Chromadorea" family="Rhabditidae" genus="Protorhabditis" kingdom="Animalia" order="Rhabditida" pageId="8" pageNumber="9" phylum="Nematoda" rank="species" species="undetermined">
<emphasis id="97F7DE09FFDBFF92AAAEFC2C6D2BFC1E" box="[162,311,966,990]" italics="true" pageId="8" pageNumber="9">Protorhabditis</emphasis>
sp.
</taxonomicName>
</heading>
</th>
</tr>
<tr id="1BB30059FFDB0065AAAEFC076991FBC5" box="[162,1421,1005,1029]" gridrow="15" pageId="8" pageNumber="9">
<th id="58626925FFDB0065AAAEFC076D44FBC5" box="[162,344,1005,1029]" gridcol="0" gridrow="15" pageId="8" pageNumber="9">Day 1</th>
<td id="58626925FFDB0065AB7EFC076E07FBC5" box="[370,539,1005,1029]" gridcol="1" gridrow="15" pageId="8" pageNumber="9">45.00 ± 5.02</td>
<td id="58626925FFDB0065A83CFC076F01FBC5" box="[560,797,1005,1029]" gridcol="2" gridrow="15" pageId="8" pageNumber="9">&lt;0.001</td>
<td id="58626925FFDB0065A93AFC076FC5FBC5" box="[822,985,1005,1029]" gridcol="3" gridrow="15" pageId="8" pageNumber="9">2.70 ± 0.30</td>
<td id="58626925FFDB0065A9F8FC0768FDFBC5" box="[1012,1249,1005,1029]" gridcol="4" gridrow="15" pageId="8" pageNumber="9">&lt;0.001</td>
<td id="58626925FFDB0065AEFBFC076991FBC5" box="[1271,1421,1005,1029]" gridcol="5" gridrow="15" pageId="8" pageNumber="9">&lt;0.001</td>
</tr>
<tr id="1BB30059FFDB0065AAAEFBFE6991FBEC" box="[162,1421,1044,1068]" gridrow="16" pageId="8" pageNumber="9">
<th id="58626925FFDB0065AAAEFBFE6D44FBEC" box="[162,344,1044,1068]" gridcol="0" gridrow="16" pageId="8" pageNumber="9">Day 2</th>
<td id="58626925FFDB0065AB7EFBFE6E07FBEC" box="[370,539,1044,1068]" gridcol="1" gridrow="16" pageId="8" pageNumber="9">95.15 ± 7.18</td>
<td id="58626925FFDB0065A83CFBFE6F01FBEC" box="[560,797,1044,1068]" gridcol="2" gridrow="16" pageId="8" pageNumber="9">&lt;0.001</td>
<td id="58626925FFDB0065A93AFBFE6FC5FBEC" box="[822,985,1044,1068]" gridcol="3" gridrow="16" pageId="8" pageNumber="9">4.21 ± 0.49</td>
<td id="58626925FFDB0065A9F8FBFE68FDFBEC" box="[1012,1249,1044,1068]" gridcol="4" gridrow="16" pageId="8" pageNumber="9">&lt;0.001</td>
<td id="58626925FFDB0065AEFBFBFE6991FBEC" box="[1271,1421,1044,1068]" gridcol="5" gridrow="16" pageId="8" pageNumber="9">&lt;0.001</td>
</tr>
<tr id="1BB30059FFDB0065AAAEFBD66991FB94" box="[162,1421,1084,1108]" gridrow="17" pageId="8" pageNumber="9">
<th id="58626925FFDB0065AAAEFBD66D44FB94" box="[162,344,1084,1108]" gridcol="0" gridrow="17" pageId="8" pageNumber="9">Day 3</th>
<td id="58626925FFDB0065AB7EFBD66E07FB94" box="[370,539,1084,1108]" gridcol="1" gridrow="17" pageId="8" pageNumber="9">18.06 ± 4.95</td>
<td id="58626925FFDB0065A83CFBD66F01FB94" box="[560,797,1084,1108]" gridcol="2" gridrow="17" pageId="8" pageNumber="9">&lt;0.001</td>
<td id="58626925FFDB0065A93AFBD66FC5FB94" box="[822,985,1084,1108]" gridcol="3" gridrow="17" pageId="8" pageNumber="9">3.23 ± 0.52</td>
<td id="58626925FFDB0065A9F8FBD668FDFB94" box="[1012,1249,1084,1108]" gridcol="4" gridrow="17" pageId="8" pageNumber="9">&lt;0.001</td>
<td id="58626925FFDB0065AEFBFBD66991FB94" box="[1271,1421,1084,1108]" gridcol="5" gridrow="17" pageId="8" pageNumber="9">&lt;0.01</td>
</tr>
<tr id="1BB30059FFDB0065AAAEFB896991FBBB" box="[162,1421,1123,1147]" gridrow="18" pageId="8" pageNumber="9">
<th id="58626925FFDB0065AAAEFB896D44FBBB" box="[162,344,1123,1147]" gridcol="0" gridrow="18" pageId="8" pageNumber="9">Day 4</th>
<td id="58626925FFDB0065AB7EFB896E07FBBB" box="[370,539,1123,1147]" gridcol="1" gridrow="18" pageId="8" pageNumber="9">2.00 ± 0.69</td>
<td id="58626925FFDB0065A83CFB896F01FBBB" box="[560,797,1123,1147]" gridcol="2" gridrow="18" pageId="8" pageNumber="9">&lt;0.01</td>
<td id="58626925FFDB0065A93AFB896FC5FBBB" box="[822,985,1123,1147]" gridcol="3" gridrow="18" pageId="8" pageNumber="9">3.12 ± 0.56</td>
<td id="58626925FFDB0065A9F8FB8968FDFBBB" box="[1012,1249,1123,1147]" gridcol="4" gridrow="18" pageId="8" pageNumber="9">&lt;0.001</td>
<td id="58626925FFDB0065AEFBFB896991FBBB" box="[1271,1421,1123,1147]" gridcol="5" gridrow="18" pageId="8" pageNumber="9">&gt;0.05</td>
</tr>
<tr id="1BB30059FFDB0065AAAEFB616991FB63" box="[162,1421,1163,1187]" gridrow="19" pageId="8" pageNumber="9">
<th id="58626925FFDB0065AAAEFB616D44FB63" box="[162,344,1163,1187]" gridcol="0" gridrow="19" pageId="8" pageNumber="9">Day 5</th>
<td id="58626925FFDB0065AB7EFB616E07FB63" box="[370,539,1163,1187]" gridcol="1" gridrow="19" pageId="8" pageNumber="9">0.88 ± 0.70</td>
<td id="58626925FFDB0065A83CFB616F01FB63" box="[560,797,1163,1187]" gridcol="2" gridrow="19" pageId="8" pageNumber="9">&gt;0.05</td>
<td id="58626925FFDB0065A93AFB616FC5FB63" box="[822,985,1163,1187]" gridcol="3" gridrow="19" pageId="8" pageNumber="9">2.38 ± 0.43</td>
<td id="58626925FFDB0065A9F8FB6168FDFB63" box="[1012,1249,1163,1187]" gridcol="4" gridrow="19" pageId="8" pageNumber="9">&lt;0.001</td>
<td id="58626925FFDB0065AEFBFB616991FB63" box="[1271,1421,1163,1187]" gridcol="5" gridrow="19" pageId="8" pageNumber="9">
<geoCoordinate id="C0B764DCFFDBFF92AF26FB616921FB63" box="[1322,1341,1163,1187]" degrees="0.001" direction="north" orientation="latitude" pageId="8" pageNumber="9" precision="55" value="0.001">N</geoCoordinate>
.A.
</td>
</tr>
<tr id="1BB30059FFDB0065AAAEFB586991FB0A" box="[162,1421,1202,1226]" gridrow="20" pageId="8" pageNumber="9">
<th id="58626925FFDB0065AAAEFB586D44FB0A" box="[162,344,1202,1226]" gridcol="0" gridrow="20" pageId="8" pageNumber="9">Day 6</th>
<td id="58626925FFDB0065AB7EFB586E07FB0A" box="[370,539,1202,1226]" gridcol="1" gridrow="20" pageId="8" pageNumber="9">0.24 ± 0.18</td>
<td id="58626925FFDB0065A83CFB586F01FB0A" box="[560,797,1202,1226]" gridcol="2" gridrow="20" pageId="8" pageNumber="9">&gt;0.05</td>
<td id="58626925FFDB0065A93AFB586FC5FB0A" box="[822,985,1202,1226]" gridcol="3" gridrow="20" pageId="8" pageNumber="9">1.36 ± 0.28</td>
<td id="58626925FFDB0065A9F8FB5868FDFB0A" box="[1012,1249,1202,1226]" gridcol="4" gridrow="20" pageId="8" pageNumber="9">&lt;0.001</td>
<td id="58626925FFDB0065AEFBFB586991FB0A" box="[1271,1421,1202,1226]" gridcol="5" gridrow="20" pageId="8" pageNumber="9">
<geoCoordinate id="C0B764DCFFDBFF92AF26FB586921FB0A" box="[1322,1341,1202,1226]" degrees="0.001" direction="north" orientation="latitude" pageId="8" pageNumber="9" precision="55" value="0.001">N</geoCoordinate>
.A.
</td>
</tr>
<tr id="1BB30059FFDB0065AAAEFB306991FB32" box="[162,1421,1242,1266]" gridrow="21" pageId="8" pageNumber="9">
<th id="58626925FFDB0065AAAEFB306D44FB32" box="[162,344,1242,1266]" gridcol="0" gridrow="21" pageId="8" pageNumber="9">Day 7</th>
<td id="58626925FFDB0065AB7EFB306E07FB32" box="[370,539,1242,1266]" gridcol="1" gridrow="21" pageId="8" pageNumber="9">0.06 ± 0.06</td>
<td id="58626925FFDB0065A83CFB306F01FB32" box="[560,797,1242,1266]" gridcol="2" gridrow="21" pageId="8" pageNumber="9">&gt;0.05</td>
<td id="58626925FFDB0065A93AFB306FC5FB32" box="[822,985,1242,1266]" gridcol="3" gridrow="21" pageId="8" pageNumber="9">1.09 ± 0.24</td>
<td id="58626925FFDB0065A9F8FB3068FDFB32" box="[1012,1249,1242,1266]" gridcol="4" gridrow="21" pageId="8" pageNumber="9">&lt;0.001</td>
<td id="58626925FFDB0065AEFBFB306991FB32" box="[1271,1421,1242,1266]" gridcol="5" gridrow="21" pageId="8" pageNumber="9">
<geoCoordinate id="C0B764DCFFDBFF92AF26FB306921FB32" box="[1322,1341,1242,1266]" degrees="0.001" direction="north" orientation="latitude" pageId="8" pageNumber="9" precision="55" value="0.001">N</geoCoordinate>
.A.
</td>
</tr>
<tr id="1BB30059FFDB0065AAAEFAEB6991FAD9" box="[162,1421,1281,1305]" gridrow="22" pageId="8" pageNumber="9" rowspan-2="1">
<th id="58626925FFDB0065AAAEFAEB6D44FAD9" box="[162,344,1281,1305]" gridcol="0" gridrow="22" pageId="8" pageNumber="9">Day 8</th>
<td id="58626925FFDB0065AB7EFAEB6E07FAD9" box="[370,539,1281,1305]" gridcol="1" gridrow="22" pageId="8" pageNumber="9">0</td>
<td id="58626925FFDB0065A93AFAEB6FC5FAD9" box="[822,985,1281,1305]" gridcol="3" gridrow="22" pageId="8" pageNumber="9">1.00 ± 0.24</td>
<td id="58626925FFDB0065A9F8FAEB68FDFAD9" box="[1012,1249,1281,1305]" gridcol="4" gridrow="22" pageId="8" pageNumber="9">&lt;0.001</td>
<td id="58626925FFDB0065AEFBFAEB6991FAD9" box="[1271,1421,1281,1305]" gridcol="5" gridrow="22" pageId="8" pageNumber="9">
<geoCoordinate id="C0B764DCFFDBFF92AF26FAEB6921FAD9" box="[1322,1341,1281,1305]" degrees="0.001" direction="north" orientation="latitude" pageId="8" pageNumber="9" precision="55" value="0.001">N</geoCoordinate>
.A.
</td>
</tr>
<tr id="1BB30059FFDB0065AAAEFAC26991FA80" box="[162,1421,1320,1344]" gridrow="23" pageId="8" pageNumber="9" rowspan-1="1" rowspan-2="1">
<th id="58626925FFDB0065AAAEFAC26D44FA80" box="[162,344,1320,1344]" gridcol="0" gridrow="23" pageId="8" pageNumber="9">Day 9</th>
<td id="58626925FFDB0065A93AFAC26FC5FA80" box="[822,985,1320,1344]" gridcol="3" gridrow="23" pageId="8" pageNumber="9">1.17 ± 0.33</td>
<td id="58626925FFDB0065A9F8FAC268FDFA80" box="[1012,1249,1320,1344]" gridcol="4" gridrow="23" pageId="8" pageNumber="9">&lt;0.01</td>
<td id="58626925FFDB0065AEFBFAC26991FA80" box="[1271,1421,1320,1344]" gridcol="5" gridrow="23" pageId="8" pageNumber="9">
<geoCoordinate id="C0B764DCFFDBFF92AF26FAC26921FA80" box="[1322,1341,1320,1344]" degrees="0.01" direction="north" orientation="latitude" pageId="8" pageNumber="9" precision="555" value="0.01">N</geoCoordinate>
.A.
</td>
</tr>
<tr id="1BB30059FFDB0065AAAEFABA6991FAA8" box="[162,1421,1360,1384]" gridrow="24" pageId="8" pageNumber="9" rowspan-1="1" rowspan-2="1">
<th id="58626925FFDB0065AAAEFABA6D44FAA8" box="[162,344,1360,1384]" gridcol="0" gridrow="24" pageId="8" pageNumber="9">Day 10</th>
<td id="58626925FFDB0065A93AFABA6FC5FAA8" box="[822,985,1360,1384]" gridcol="3" gridrow="24" pageId="8" pageNumber="9">0.53 ± 0.18</td>
<td id="58626925FFDB0065A9F8FABA68FDFAA8" box="[1012,1249,1360,1384]" gridcol="4" gridrow="24" pageId="8" pageNumber="9">&lt;0.01</td>
<td id="58626925FFDB0065AEFBFABA6991FAA8" box="[1271,1421,1360,1384]" gridcol="5" gridrow="24" pageId="8" pageNumber="9">
<geoCoordinate id="C0B764DCFFDBFF92AF26FABA6921FAA8" box="[1322,1341,1360,1384]" degrees="0.01" direction="north" orientation="latitude" pageId="8" pageNumber="9" precision="555" value="0.01">N</geoCoordinate>
.A.
</td>
</tr>
<tr id="1BB30059FFDB0065AAAEFA9D6991FA4F" box="[162,1421,1399,1423]" gridrow="25" pageId="8" pageNumber="9" rowspan-1="1" rowspan-2="1">
<th id="58626925FFDB0065AAAEFA9D6D44FA4F" box="[162,344,1399,1423]" gridcol="0" gridrow="25" pageId="8" pageNumber="9">Day 11</th>
<td id="58626925FFDB0065A93AFA9D6FC5FA4F" box="[822,985,1399,1423]" gridcol="3" gridrow="25" pageId="8" pageNumber="9">0.53 ± 0.19</td>
<td id="58626925FFDB0065A9F8FA9D68FDFA4F" box="[1012,1249,1399,1423]" gridcol="4" gridrow="25" pageId="8" pageNumber="9">&lt;0.01</td>
<td id="58626925FFDB0065AEFBFA9D6991FA4F" box="[1271,1421,1399,1423]" gridcol="5" gridrow="25" pageId="8" pageNumber="9">
<geoCoordinate id="C0B764DCFFDBFF92AF26FA9D6921FA4F" box="[1322,1341,1399,1423]" degrees="0.01" direction="north" orientation="latitude" pageId="8" pageNumber="9" precision="555" value="0.01">N</geoCoordinate>
.A.
</td>
</tr>
<tr id="1BB30059FFDB0065AAAEFA756991FA77" box="[162,1421,1439,1463]" gridrow="26" pageId="8" pageNumber="9" rowspan-1="1" rowspan-2="1">
<th id="58626925FFDB0065AAAEFA756D44FA77" box="[162,344,1439,1463]" gridcol="0" gridrow="26" pageId="8" pageNumber="9">Day 12</th>
<td id="58626925FFDB0065A93AFA756FC5FA77" box="[822,985,1439,1463]" gridcol="3" gridrow="26" pageId="8" pageNumber="9">0.42 ± 0.14</td>
<td id="58626925FFDB0065A9F8FA7568FDFA77" box="[1012,1249,1439,1463]" gridcol="4" gridrow="26" pageId="8" pageNumber="9">&lt;0.01</td>
<td id="58626925FFDB0065AEFBFA756991FA77" box="[1271,1421,1439,1463]" gridcol="5" gridrow="26" pageId="8" pageNumber="9">
<geoCoordinate id="C0B764DCFFDBFF92AF26FA756921FA77" box="[1322,1341,1439,1463]" degrees="0.01" direction="north" orientation="latitude" pageId="8" pageNumber="9" precision="555" value="0.01">N</geoCoordinate>
.A.
</td>
</tr>
<tr id="1BB30059FFDB0065AAAEFA2C6991FA1E" box="[162,1421,1478,1502]" gridrow="27" pageId="8" pageNumber="9" rowspan-1="1" rowspan-2="1">
<th id="58626925FFDB0065AAAEFA2C6D44FA1E" box="[162,344,1478,1502]" gridcol="0" gridrow="27" pageId="8" pageNumber="9">Day 13</th>
<td id="58626925FFDB0065A93AFA2C6FC5FA1E" box="[822,985,1478,1502]" gridcol="3" gridrow="27" pageId="8" pageNumber="9">0.14 ± 0.06</td>
<td id="58626925FFDB0065A9F8FA2C68FDFA1E" box="[1012,1249,1478,1502]" gridcol="4" gridrow="27" pageId="8" pageNumber="9">&lt;0.05</td>
<td id="58626925FFDB0065AEFBFA2C6991FA1E" box="[1271,1421,1478,1502]" gridcol="5" gridrow="27" pageId="8" pageNumber="9">
<geoCoordinate id="C0B764DCFFDBFF92AF26FA2C6921FA1E" box="[1322,1341,1478,1502]" degrees="0.05" direction="north" orientation="latitude" pageId="8" pageNumber="9" precision="555" value="0.05">N</geoCoordinate>
.A.
</td>
</tr>
<tr id="1BB30059FFDB0065AAAEFA076991F9C5" box="[162,1421,1517,1541]" gridrow="28" pageId="8" pageNumber="9" rowspan-1="1" rowspan-2="1">
<th id="58626925FFDB0065AAAEFA076D44F9C5" box="[162,344,1517,1541]" gridcol="0" gridrow="28" pageId="8" pageNumber="9">Day 14</th>
<td id="58626925FFDB0065A93AFA076FC5F9C5" box="[822,985,1517,1541]" gridcol="3" gridrow="28" pageId="8" pageNumber="9">0.10 ± 0.07</td>
<td id="58626925FFDB0065A9F8FA0768FDF9C5" box="[1012,1249,1517,1541]" gridcol="4" gridrow="28" pageId="8" pageNumber="9">&gt;0.05</td>
<td id="58626925FFDB0065AEFBFA076991F9C5" box="[1271,1421,1517,1541]" gridcol="5" gridrow="28" pageId="8" pageNumber="9">
<geoCoordinate id="C0B764DCFFDBFF92AF26FA076921F9C5" box="[1322,1341,1517,1541]" degrees="0.05" direction="north" orientation="latitude" pageId="8" pageNumber="9" precision="555" value="0.05">N</geoCoordinate>
.A.
</td>
</tr>
<tr id="1BB30059FFDB0065AAAEF9FF6991F9ED" box="[162,1421,1557,1581]" gridrow="29" pageId="8" pageNumber="9" rowspan-1="1" rowspan-2="1">
<th id="58626925FFDB0065AAAEF9FF6D44F9ED" box="[162,344,1557,1581]" gridcol="0" gridrow="29" pageId="8" pageNumber="9">Day 15</th>
<td id="58626925FFDB0065A93AF9FF6FC5F9ED" box="[822,985,1557,1581]" gridcol="3" gridrow="29" pageId="8" pageNumber="9">0.06 ± 0.04</td>
<td id="58626925FFDB0065A9F8F9FF68FDF9ED" box="[1012,1249,1557,1581]" gridcol="4" gridrow="29" pageId="8" pageNumber="9">&gt;0.05</td>
<td id="58626925FFDB0065AEFBF9FF6991F9ED" box="[1271,1421,1557,1581]" gridcol="5" gridrow="29" pageId="8" pageNumber="9">
<geoCoordinate id="C0B764DCFFDBFF92AF26F9FF6921F9ED" box="[1322,1341,1557,1581]" degrees="0.05" direction="north" orientation="latitude" pageId="8" pageNumber="9" precision="555" value="0.05">N</geoCoordinate>
.A.
</td>
</tr>
<tr id="1BB30059FFDB0065AAAEF9D66991F994" box="[162,1421,1596,1620]" gridrow="30" pageId="8" pageNumber="9">
<th id="58626925FFDB0065AAAEF9D66991F994" box="[162,1421,1596,1620]" colspan="6" colspanRight="5" gridcol="0" gridrow="30" pageId="8" pageNumber="9">0</th>
</tr>
<tr id="1BB30059FFDB0065AAAEF9896991F9BC" box="[162,1421,1635,1660]" gridrow="31" pageId="8" pageNumber="9" rowspan-2="1" rowspan-4="1" rowspan-5="1">
<th id="58626925FFDB0065AAAEF9896D44F9BC" box="[162,344,1635,1660]" gridcol="0" gridrow="31" pageId="8" pageNumber="9">
<emphasis id="97F7DE09FFDBFF92AAAEF98E6CB3F9BC" box="[162,175,1636,1660]" italics="true" pageId="8" pageNumber="9">n</emphasis>
<superScript id="52F6AF53FFDBFF92AAA3F9896CABF9B1" attach="left" box="[175,183,1635,1649]" fontSize="6" pageId="8" pageNumber="9">a</superScript>
</th>
<td id="58626925FFDB0065AB7EF9896E07F9BC" box="[370,539,1635,1660]" gridcol="1" gridrow="31" pageId="8" pageNumber="9">34</td>
<td id="58626925FFDB0065A93AF9896FC5F9BC" box="[822,985,1635,1660]" gridcol="3" gridrow="31" pageId="8" pageNumber="9">47</td>
</tr>
<tr id="1BB30059FFDB0065AAAEF9616991F901" box="[162,1421,1675,1729]" gridrow="32" pageId="8" pageNumber="9" rowspan-2="1" rowspan-3="1">
<th id="58626925FFDB0065AAAEF9616D44F901" box="[162,344,1675,1729]" gridcol="0" gridrow="32" pageId="8" pageNumber="9">
Average brood size per 
<superScript id="52F6AF53FFDBFF92AAF1F9436D5FF976" attach="left" box="[253,323,1704,1729]" fontSize="6" pageId="8" pageNumber="9">wormb</superScript>
</th>
<td id="58626925FFDB0065AB7EF9616E07F901" box="[370,539,1675,1729]" gridcol="1" gridrow="32" pageId="8" pageNumber="9">161.38 ± 10.05</td>
<td id="58626925FFDB0065A9F8F96168FDF901" box="[1012,1249,1675,1729]" gridcol="4" gridrow="32" pageId="8" pageNumber="9">22.08 ± 2.26</td>
<td id="58626925FFDB0065AEFBF9616991F901" box="[1271,1421,1675,1729]" gridcol="5" gridrow="32" pageId="8" pageNumber="9">&lt;0.01</td>
</tr>
<tr id="1BB30059FFDB0065AAAEF9256991F8C5" box="[162,1421,1743,1797]" gridrow="33" pageId="8" pageNumber="9" rowspan-2="1" rowspan-3="1">
<th id="58626925FFDB0065AAAEF9256D44F8C5" box="[162,344,1743,1797]" gridcol="0" gridrow="33" pageId="8" pageNumber="9">
RS (day) 
<superScript id="52F6AF53FFDBFF92AAF1F9256D19F91D" attach="left" box="[253,261,1743,1757]" fontSize="6" pageId="8" pageNumber="9">c</superScript>
</th>
<td id="58626925FFDB0065AB7EF9256E07F8C5" box="[370,539,1743,1797]" gridcol="1" gridrow="33" pageId="8" pageNumber="9">3.59 ± 0.20</td>
<td id="58626925FFDB0065A9F8F92568FDF8C5" box="[1012,1249,1743,1797]" gridcol="4" gridrow="33" pageId="8" pageNumber="9">6.68 ± 0.61</td>
<td id="58626925FFDB0065AEFBF9256991F8C5" box="[1271,1421,1743,1797]" gridcol="5" gridrow="33" pageId="8" pageNumber="9">&lt;0.001 (log-rank test)</td>
</tr>
</table>
</paragraph>
<subSection id="CC0C1BF0FFDBFF92AA9BF8F56E75F863" pageId="8" pageNumber="9" type="multiple">
<paragraph id="A53C021BFFDBFF92AA9BF8F56EC4F8F8" blockId="8.[151,1114,1823,1955]" box="[151,728,1823,1848]" pageId="8" pageNumber="9">
<superScript id="52F6AF53FFDBFF92AA9BF8F56CACF8F8" attach="right" box="[151,176,1823,1848]" fontSize="6" pageId="8" pageNumber="9">an</superScript>
, the total number of tested individuals in each test.
</paragraph>
<paragraph id="A53C021BFFDBFF92AA9BF8A96846F89B" blockId="8.[151,1114,1823,1955]" box="[151,1114,1859,1883]" pageId="8" pageNumber="9">
<superScript id="52F6AF53FFDBFF92AA9BF8A96CE5F89B" attach="right" box="[151,249,1859,1883]" fontSize="6" pageId="8" pageNumber="9">bAverage</superScript>
brood size per worm was the total brood of all hermaphrodites (n) divided by n.
</paragraph>
<paragraph id="A53C021BFFDBFF92AA9BF88C6E11F8BF" blockId="8.[151,1114,1823,1955]" box="[151,525,1894,1919]" pageId="8" pageNumber="9">
<superScript id="52F6AF53FFDBFF92AA9BF88C6CE4F8BF" attach="right" box="[151,248,1894,1919]" fontSize="6" pageId="8" pageNumber="9">cAverage</superScript>
RS. N.A., not applicable.
</paragraph>
<paragraph id="A53C021BFFDBFF92AA9BF8616E75F863" blockId="8.[151,1114,1823,1955]" box="[151,617,1931,1955]" pageId="8" pageNumber="9">RS, reproductive span; SE, standard error.</paragraph>
</subSection>
<caption id="F1FC5293FFDAFF93AB44FC656EE5FB88" ID-DOI="http://doi.org/10.5281/zenodo.12175632" ID-Zenodo-Dep="12175632" httpUri="https://zenodo.org/record/12175632/files/figure.png" pageId="9" pageNumber="10" startId="9.[328,399,911,936]" targetBox="[173,773,186,867]" targetPageId="9" targetType="figure">
<paragraph id="A53C021BFFDAFF93AB44FC656EE5FB88" blockId="9.[326,773,911,1096]" pageId="9" pageNumber="10">
Figure 2: Lifespan assays of 
<taxonomicName id="62837998FFDAFF93A881FC656F19FC68" authorityName="Bristol" authorityYear="2022" box="[653,773,911,936]" class="Chromadorea" family="Rhabditidae" genus="Caenorhabditis" kingdom="Animalia" order="Rhabditida" pageId="9" pageNumber="10" phylum="Nematoda" rank="species" species="elegans">
<emphasis id="97F7DE09FFDAFF93A881FC656F19FC68" box="[653,773,911,936]" italics="true" pageId="9" pageNumber="10">C. elegans</emphasis>
</taxonomicName>
on 
<taxonomicName id="62837998FFDAFF93AB67FC456E5AFC08" box="[363,582,943,968]" class="Actinobacteridae" family="Microbacteriaceae" genus="Microbacterium" kingdom="Bacteria" order="Actinomycetales" pageId="9" pageNumber="10" phylum="Actinobacteria" rank="species" species="undetermined">
<emphasis id="97F7DE09FFDAFF93AB67FC456E07FC08" box="[363,539,943,968]" italics="true" pageId="9" pageNumber="10">Microbacterium</emphasis>
sp.
</taxonomicName>
CFBb37 and the OP50 control. 
<taxonomicName id="62837998FFDAFF93A815FC256E8DFC28" authorityName="Bristol" authorityYear="2022" box="[537,657,975,1000]" class="Chromadorea" family="Rhabditidae" genus="Caenorhabditis" kingdom="Animalia" order="Rhabditida" pageId="9" pageNumber="10" phylum="Nematoda" rank="species" species="elegans">
<emphasis id="97F7DE09FFDAFF93A815FC256E8DFC28" box="[537,657,975,1000]" italics="true" pageId="9" pageNumber="10">C. elegans</emphasis>
</taxonomicName>
grown on CFBb37 survived longer than OP50 (
<emphasis id="97F7DE09FFDAFF93AB41FBE56D42FBE8" box="[333,350,1039,1064]" italics="true" pageId="9" pageNumber="10">P</emphasis>
&lt;0.001, log-rank test). Detailed lifespan data are in Tables S4 and S5.
</paragraph>
</caption>
<paragraph id="A53C021BFFDAFF93AAAAFB986E59FB77" blockId="9.[166,772,1138,1207]" pageId="9" pageNumber="10">
<heading id="FE74B577FFDAFF93AAAAFB986E59FB77" centered="true" fontSize="12" level="2" pageId="9" pageNumber="10" reason="2">
<emphasis id="97F7DE09FFDAFF93AAAAFB986E59FB77" bold="true" pageId="9" pageNumber="10">
Brood sizes of closely related species on 
<taxonomicName id="62837998FFDAFF93AAAAFB736DD9FB77" box="[166,453,1177,1207]" class="Actinobacteridae" family="Microbacteriaceae" genus="Microbacterium" kingdom="Bacteria" order="Actinomycetales" pageId="9" pageNumber="10" phylum="Actinobacteria" rank="species" species="undetermined">Microbacterium sp.</taxonomicName>
CFBb37
</emphasis>
</heading>
</paragraph>
<paragraph id="A53C021BFFDAFF93AAAAFB396DE9F90C" blockId="9.[165,784,1235,1740]" pageId="9" pageNumber="10">
The extension of RS in 
<taxonomicName id="62837998FFDAFF93ABBAFB396E2CFB2C" authorityName="Bristol" authorityYear="2022" box="[438,560,1235,1260]" class="Chromadorea" family="Rhabditidae" genus="Caenorhabditis" kingdom="Animalia" order="Rhabditida" pageId="9" pageNumber="10" phylum="Nematoda" rank="species" species="elegans">
<emphasis id="97F7DE09FFDAFF93ABBAFB396E2CFB2C" box="[438,560,1235,1260]" italics="true" pageId="9" pageNumber="10">C. elegans</emphasis>
</taxonomicName>
by 
<taxonomicName id="62837998FFDAFF93A850FB396CD6FACC" class="Actinobacteridae" family="Microbacteriaceae" genus="Microbacterium" kingdom="Bacteria" order="Actinomycetales" pageId="9" pageNumber="10" phylum="Actinobacteria" rank="species" species="undetermined">
<emphasis id="97F7DE09FFDAFF93A850FB396F12FB2C" box="[604,782,1235,1260]" italics="true" pageId="9" pageNumber="10">Microbacterium</emphasis>
sp.
</taxonomicName>
CBFb37 was associated with a decrease in brood size. We next examined whether the extension of RS in the three other species was also associated with a consistent decrease in brood size. We found that the brood sizes of both 
<taxonomicName id="62837998FFDAFF93ABD2FA996E74FA4C" box="[478,616,1395,1420]" class="Chromadorea" family="Rhabditidae" genus="Caenorhabditis" kingdom="Animalia" order="Rhabditida" pageId="9" pageNumber="10" phylum="Nematoda" rank="species" species="briggsae">
<emphasis id="97F7DE09FFDAFF93ABD2FA996E74FA4C" box="[478,616,1395,1420]" italics="true" pageId="9" pageNumber="10">C. briggsae</emphasis>
</taxonomicName>
(16.00 ± 1.88 
<taxonomicName id="62837998FFDAFF93AAAAFA796D8DFA6C" box="[166,401,1427,1452]" class="Actinobacteridae" family="Microbacteriaceae" genus="Microbacterium" kingdom="Bacteria" order="Actinomycetales" pageId="9" pageNumber="10" phylum="Actinobacteria" rank="species" species="undetermined">
<emphasis id="97F7DE09FFDAFF93AAAAFA796D44FA6C" box="[166,344,1427,1452]" italics="true" pageId="9" pageNumber="10">Microbacterium</emphasis>
sp.
</taxonomicName>
CBFb37 versus 65.40 ± 
<geoCoordinate id="C0B764DCFFDAFF93A8D0FA796CA5FA0C" degrees="7.09" direction="east" orientation="longitude" pageId="9" pageNumber="10" precision="555" value="7.09">
7.09 
<taxonomicName id="62837998FFDAFF93AAAAFA596D3DFA0C" authority="OP" authorityName="OP" box="[166,289,1459,1484]" class="Gammaproteobacteria" family="Enterobacteriaceae" genus="Escherichia" kingdom="Bacteria" order="Enterobacteriales" pageId="9" pageNumber="10" phylum="Proteobacteria" rank="species" species="coli">
<emphasis id="97F7DE09FFDAFF93AAAAFA596CF2FA0C" box="[166,238,1459,1484]" italics="true" pageId="9" pageNumber="10">E. coli</emphasis>
OP
</taxonomicName>
</geoCoordinate>
50; 
<emphasis id="97F7DE09FFDAFF93AB42FA596D43FA0C" box="[334,351,1459,1484]" italics="true" pageId="9" pageNumber="10">P</emphasis>
&lt;0.001; 
<tableCitation id="E80137A0FFDAFF93ABC2FA596E39FA0C" box="[462,549,1459,1484]" captionStart="Table 2" captionStartId="7.[166,248,184,214]" captionTargetBox="[177,1436,268,1903]" captionText="Table 2. Total average brood and RS of tested nematode species." httpUri="http://table.plazi.org/id/F1FC5293FFD4FF9DAAAAFF52686DFF16" pageId="9" pageNumber="10" tableUuid="F1FC5293FFD4FF9DAAAAFF52686DFF16">Table 2</tableCitation>
) and 
<taxonomicName id="62837998FFDAFF93A863FA596CD6FA2C" class="Chromadorea" family="Rhabditidae" genus="Protorhabditis" kingdom="Animalia" order="Rhabditida" pageId="9" pageNumber="10" phylum="Nematoda" rank="species" species="undetermined">
<emphasis id="97F7DE09FFDAFF93A863FA596F11FA0C" box="[623,781,1459,1484]" italics="true" pageId="9" pageNumber="10">Protorhabditis</emphasis>
sp.
</taxonomicName>
(22.08 ± 2.26 versus 161.38 ± 10.05; 
<emphasis id="97F7DE09FFDAFF93A8ABFA396EA4FA2C" box="[679,696,1491,1516]" italics="true" pageId="9" pageNumber="10">P</emphasis>
&lt;0.01; 
<tableCitation id="E80137A0FFDAFF93AAAAFA196CE4F9CC" box="[166,248,1523,1548]" captionStart="Table 2" captionStartId="7.[166,248,184,214]" captionTargetBox="[177,1436,268,1903]" captionText="Table 2. Total average brood and RS of tested nematode species." httpUri="http://table.plazi.org/id/F1FC5293FFD4FF9DAAAAFF52686DFF16" pageId="9" pageNumber="10" tableUuid="F1FC5293FFD4FF9DAAAAFF52686DFF16">Table 2</tableCitation>
) on 
<taxonomicName id="62837998FFDAFF93AB24FA196E19F9CC" box="[296,517,1523,1548]" class="Actinobacteridae" family="Microbacteriaceae" genus="Microbacterium" kingdom="Bacteria" order="Actinomycetales" pageId="9" pageNumber="10" phylum="Actinobacteria" rank="species" species="undetermined">
<emphasis id="97F7DE09FFDAFF93AB24FA196DC6F9CC" box="[296,474,1523,1548]" italics="true" pageId="9" pageNumber="10">Microbacterium</emphasis>
sp.
</taxonomicName>
CFBb37 was less than on 
<taxonomicName id="62837998FFDAFF93AAC0F9F96D5DF9EC" authority="OP" authorityName="OP" box="[204,321,1555,1580]" class="Gammaproteobacteria" family="Enterobacteriaceae" genus="Escherichia" kingdom="Bacteria" order="Enterobacteriales" pageId="9" pageNumber="10" phylum="Proteobacteria" rank="species" species="coli">
<emphasis id="97F7DE09FFDAFF93AAC0F9F96D0CF9EC" box="[204,272,1555,1580]" italics="true" pageId="9" pageNumber="10">E. coli</emphasis>
OP
</taxonomicName>
50. However, 
<taxonomicName id="62837998FFDAFF93ABD3F9F96E7AF9EC" box="[479,614,1555,1580]" class="Chromadorea" family="Rhabditidae" genus="Caenorhabditis" kingdom="Animalia" order="Rhabditida" pageId="9" pageNumber="10" phylum="Nematoda" rank="species" species="tropicalis">
<emphasis id="97F7DE09FFDAFF93ABD3F9F96E7AF9EC" box="[479,614,1555,1580]" italics="true" pageId="9" pageNumber="10">C. tropicalis</emphasis>
</taxonomicName>
brood size on 
<taxonomicName id="62837998FFDAFF93AAABF9D96D9FF98C" box="[167,387,1587,1612]" class="Actinobacteridae" family="Microbacteriaceae" genus="Microbacterium" kingdom="Bacteria" order="Actinomycetales" pageId="9" pageNumber="10" phylum="Actinobacteria" rank="species" species="undetermined">
<emphasis id="97F7DE09FFDAFF93AAABF9D96D45F98C" box="[167,345,1587,1612]" italics="true" pageId="9" pageNumber="10">Microbacterium</emphasis>
sp.
</taxonomicName>
CFBb37 was more than on 
<taxonomicName id="62837998FFDAFF93A8C5F9D96CD0F9AC" authority="OP" authorityName="OP" class="Gammaproteobacteria" family="Enterobacteriaceae" genus="Escherichia" kingdom="Bacteria" order="Enterobacteriales" pageId="9" pageNumber="10" phylum="Proteobacteria" rank="species" species="coli">
<emphasis id="97F7DE09FFDAFF93A8C5F9D96F10F98C" box="[713,780,1587,1612]" italics="true" pageId="9" pageNumber="10">E. coli</emphasis>
OP
</taxonomicName>
50 (25.25 ± 3.59 versus 10.78 ± 1.90; 
<emphasis id="97F7DE09FFDAFF93A8ABF9B96EA4F9AC" box="[679,696,1619,1644]" italics="true" pageId="9" pageNumber="10">P</emphasis>
&lt;0.01; 
<tableCitation id="E80137A0FFDAFF93AAAAF9996D1FF94C" box="[166,259,1651,1676]" captionStart="Table 2" captionStartId="7.[166,248,184,214]" captionTargetBox="[177,1436,268,1903]" captionText="Table 2. Total average brood and RS of tested nematode species." httpUri="http://table.plazi.org/id/F1FC5293FFD4FF9DAAAAFF52686DFF16" pageId="9" pageNumber="10" tableUuid="F1FC5293FFD4FF9DAAAAFF52686DFF16">Table 2</tableCitation>
). Together, these results suggest that the interaction between 
<taxonomicName id="62837998FFDAFF93AB98F9796E6DF96C" box="[404,625,1683,1708]" class="Actinobacteridae" family="Microbacteriaceae" genus="Microbacterium" kingdom="Bacteria" order="Actinomycetales" pageId="9" pageNumber="10" phylum="Actinobacteria" rank="species" species="undetermined">
<emphasis id="97F7DE09FFDAFF93AB98F9796E5AF96C" box="[404,582,1683,1708]" italics="true" pageId="9" pageNumber="10">Microbacterium</emphasis>
sp.
</taxonomicName>
CFBb37 and the nematodes is not simple.
</paragraph>
</subSubSection>
</treatment>
</document>