223 lines
15 KiB
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223 lines
15 KiB
XML
<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.77.59852" ID-GBIF-Dataset="78bab002-8c3e-4548-8377-cb082fa11d13" ID-PMC="PMC7819953" ID-Pensoft-Pub="1314-4049-77-65" ID-Pensoft-UUID="5D8D5A6329A4584ABC11C425E4B0E47A" ID-PubMed="33519269" ModsDocID="1314-4049-77-65" checkinTime="1610662561303" checkinUser="pensoft" docAuthor="Sun, Wenxiu, Huang, Shengting, Xia, Jiwen, Zhang, Xiuguo & Li, Zhuang" docDate="2021" docId="DF618B9FFD4E5BD1A07581F27B2272B0" docLanguage="en" docName="MycoKeys 77: 65-95" docOrigin="MycoKeys 77" docPubDate="2021-01-14" docSource="http://dx.doi.org/10.3897/mycokeys.77.59852" docTitle="Diaporthe heterostemmatis S. T. Huang, J. W. Xia, X. G. Zhang & Z. Li 2021, sp. nov." docType="treatment" docVersion="4" id="5D8D5A6329A4584ABC11C425E4B0E47A" lastPageNumber="65" masterDocId="5D8D5A6329A4584ABC11C425E4B0E47A" masterDocTitle="Morphological and molecular identification of Diaporthe species in south-western China, with description of eight new species" masterLastPageNumber="95" masterPageNumber="65" pageNumber="65" updateTime="1668136608950" updateUser="ExternalLinkService">
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<mods:mods xmlns:mods="http://www.loc.gov/mods/v3">
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<mods:titleInfo>
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<mods:title>Morphological and molecular identification of Diaporthe species in south-western China, with description of eight new species</mods:title>
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</mods:titleInfo>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Sun, Wenxiu</mods:namePart>
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<mods:affiliation>College of Life Sciences, Yangtze University, Jingzhou 434025, Hubei, China</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Huang, Shengting</mods:namePart>
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<mods:affiliation>College of Life Sciences, Yangtze University, Jingzhou 434025, Hubei, China</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Xia, Jiwen</mods:namePart>
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<mods:nameIdentifier type="ORCID">https://orcid.org/0000-0002-7436-7249</mods:nameIdentifier>
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<mods:affiliation>Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Taian, Shandong, 271018, China</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Zhang, Xiuguo</mods:namePart>
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<mods:affiliation>Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Taian, Shandong, 271018, China</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Li, Zhuang</mods:namePart>
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<mods:affiliation>Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Taian, Shandong, 271018, China</mods:affiliation>
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<mods:nameIdentifier type="email">junwuxue@126.com</mods:nameIdentifier>
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</mods:name>
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<mods:typeOfResource>text</mods:typeOfResource>
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<mods:relatedItem type="host">
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<mods:titleInfo>
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<mods:title>MycoKeys</mods:title>
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</mods:titleInfo>
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<mods:part>
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<mods:date>2021</mods:date>
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<mods:detail type="pubDate">
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<mods:number>2021-01-14</mods:number>
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</mods:detail>
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<mods:detail type="volume">
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<mods:number>77</mods:number>
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</mods:detail>
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<mods:extent unit="page">
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<mods:start>65</mods:start>
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<mods:end>95</mods:end>
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<mods:location>
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<mods:url>http://dx.doi.org/10.3897/mycokeys.77.59852</mods:url>
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</mods:location>
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<mods:classification>journal article</mods:classification>
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<mods:identifier type="DOI">http://dx.doi.org/10.3897/mycokeys.77.59852</mods:identifier>
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<mods:identifier type="Pensoft-Pub">1314-4049-77-65</mods:identifier>
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<treatment ID-GBIF-Taxon="176584075" LSID="urn:lsid:plazi:treatment:DF618B9FFD4E5BD1A07581F27B2272B0" httpUri="http://treatment.plazi.org/id/DF618B9FFD4E5BD1A07581F27B2272B0" lastPageNumber="65" pageId="0" pageNumber="65">
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<subSubSection pageId="0" pageNumber="65" type="nomenclature">
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<paragraph pageId="0" pageNumber="65">
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<taxonomicName LSID="DF618B9F-FD4E-5BD1-A075-81F27B2272B0" authority="S. T. Huang, J. W. Xia, X. G. Zhang & Z. Li" authorityName="S. T. Huang, J. W. Xia, X. G. Zhang & Z. Li" authorityYear="2021" class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe heterostemmatis" order="Diaporthales" pageId="0" pageNumber="65" phylum="Ascomycota" rank="species" species="heterostemmatis" status="sp. nov.">Diaporthe heterostemmatis S.T. Huang, J.W. Xia, X.G. Zhang & Z. Li</taxonomicName>
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<taxonomicNameLabel pageId="0" pageNumber="65">sp. nov.</taxonomicNameLabel>
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<figureCitation captionStart="Figure 5" captionStartId="F6" captionText="Figure 5. Diaporthe heterostemmatis (SAUCC 194.85) a leaf of host plant b, c surface (b) and reverse (c) sides of colony, after incubation for 15 days on PDA d conidiomata on PDA e, f conidiophores and conidiogenous cells g beta conidia h Alpha conidia i, j alpha conidia and beta conidia. Scale bars: 10 μm (e-j)." figureDoi="10.3897/mycokeys.77.59852.figure5" httpUri="https://binary.pensoft.net/fig/497508" pageId="0" pageNumber="65">Figure 5</figureCitation>
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="65" type="etymology">
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<paragraph pageId="0" pageNumber="65">Etymology.</paragraph>
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<paragraph pageId="0" pageNumber="65">
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Named after the host
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<taxonomicName authorityName="Costantin" authorityYear="1912" class="Dicotyledoneae" family="Asclepiadaceae" genus="Heterostemma" higherTaxonomySource="GBIF,IPNI" kingdom="Plantae" lsidName="Heterostemma grandiflorum" order="Gentianales" pageId="0" pageNumber="65" phylum="Angiospermae" rank="species" species="grandiflorum">
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<emphasis italics="true" pageId="0" pageNumber="65">Heterostemma grandiflorum</emphasis>
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</taxonomicName>
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on which it was collected.
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="65" type="diagnosis">
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<paragraph pageId="0" pageNumber="65">Diagnosis.</paragraph>
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<paragraph pageId="0" pageNumber="65">
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<taxonomicName authorityName="S. T. Huang, J. W. Xia, X. G. Zhang & Z. Li" authorityYear="2021" class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe heterostemmatis" order="Diaporthales" pageId="0" pageNumber="65" phylum="Ascomycota" rank="species" species="heterostemmatis">
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<emphasis italics="true" pageId="0" pageNumber="65">Diaporthe heterostemmatis</emphasis>
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</taxonomicName>
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differs from its closest phylogenetic species
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<taxonomicName lsidName="D. subellipicola" pageId="0" pageNumber="65" rank="species" species="subellipicola">
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<emphasis italics="true" pageId="0" pageNumber="65">D. subellipicola</emphasis>
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</taxonomicName>
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S.K. Huang & K.D. Hyde in ITS,
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<emphasis italics="true" pageId="0" pageNumber="65">TUB</emphasis>
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and
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<emphasis italics="true" pageId="0" pageNumber="65">TEF</emphasis>
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loci based on the alignments deposited in Tree-BASE.
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</paragraph>
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<caption doi="10.3897/mycokeys.77.59852.figure5" httpUri="https://binary.pensoft.net/fig/497508" pageId="0" pageNumber="65" start="Figure 5" startId="F6">
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<paragraph pageId="0" pageNumber="65">
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<emphasis bold="true" pageId="0" pageNumber="65">Figure 5.</emphasis>
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<taxonomicName authorityName="S. T. Huang, J. W. Xia, X. G. Zhang & Z. Li" authorityYear="2021" class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe heterostemmatis" order="Diaporthales" pageId="0" pageNumber="65" phylum="Ascomycota" rank="species" species="heterostemmatis">
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<emphasis italics="true" pageId="0" pageNumber="65">Diaporthe heterostemmatis</emphasis>
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</taxonomicName>
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(SAUCC194.85)
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<emphasis bold="true" pageId="0" pageNumber="65">a</emphasis>
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leaf of host plant
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<emphasis bold="true" pageId="0" pageNumber="65">b, c</emphasis>
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surface (
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<emphasis bold="true" pageId="0" pageNumber="65">b</emphasis>
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) and reverse (
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<emphasis bold="true" pageId="0" pageNumber="65">c</emphasis>
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) sides of colony, after incubation for 15 days on PDA
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<emphasis bold="true" pageId="0" pageNumber="65">d</emphasis>
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conidiomata on PDA
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<emphasis bold="true" pageId="0" pageNumber="65">e, f</emphasis>
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conidiophores and conidiogenous cells
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<emphasis bold="true" pageId="0" pageNumber="65">g</emphasis>
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beta conidia
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<emphasis bold="true" pageId="0" pageNumber="65">h</emphasis>
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Alpha conidia
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<emphasis bold="true" pageId="0" pageNumber="65">i, j</emphasis>
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alpha conidia and beta conidia. Scale bars: 10
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<normalizedToken originalValue="μm">μm</normalizedToken>
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(
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<emphasis bold="true" pageId="0" pageNumber="65">e-j</emphasis>
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).
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</paragraph>
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</caption>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="65" type="materials_examined">
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<paragraph pageId="0" pageNumber="65">Type.</paragraph>
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<paragraph pageId="0" pageNumber="65">
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China, Yunnan Province: Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, on infected leaves of
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<taxonomicName authorityName="Costantin" authorityYear="1912" class="Dicotyledoneae" family="Asclepiadaceae" genus="Heterostemma" higherTaxonomySource="GBIF,IPNI" kingdom="Plantae" lsidName="Heterostemma grandiflorum" order="Gentianales" pageId="0" pageNumber="65" phylum="Angiospermae" rank="species" species="grandiflorum">
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<emphasis italics="true" pageId="0" pageNumber="65">Heterostemma grandiflorum</emphasis>
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</taxonomicName>
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. 19 April 2019, S.T. Huang, HSAUP194.85, holotype, ex-holotype living culture SAUCC194.85.
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="65" type="description">
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<paragraph pageId="0" pageNumber="65">Description.</paragraph>
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<paragraph pageId="0" pageNumber="65">
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Asexual morph: Conidiomata pycnidial, 3-5 pycnidia grouped together, globose, black, erumpent, exuding creamy to yellowish conidial droplets from ostioles. Conidiophores hyaline, septate, branched, elliptical or cylindrical, straight to sinuous, 6.5-10.5
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<normalizedToken originalValue="×">x</normalizedToken>
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2.5-4.5
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<normalizedToken originalValue="μm">μm</normalizedToken>
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. Conidiogenous cells 5.3-11.8
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<normalizedToken originalValue="×">x</normalizedToken>
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1.5-3.2
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<normalizedToken originalValue="μm">μm</normalizedToken>
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, phialidic, cylindrical, enlarged towards the base, tapering towards the apex, slightly curved, neck up to 5.5
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<normalizedToken originalValue="μm">μm</normalizedToken>
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long, 2.0
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<normalizedToken originalValue="μm">μm</normalizedToken>
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wide. Alpha conidia, hyaline, smooth, aseptate, ellipsoidal, biguttulate, apex subobtuse, base subtruncate, 5.8-7.5
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<normalizedToken originalValue="×">x</normalizedToken>
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2.5-3.3
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<normalizedToken originalValue="µm">µm</normalizedToken>
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(mean = 6.5
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<normalizedToken originalValue="×">x</normalizedToken>
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3.0
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<normalizedToken originalValue="μm">μm</normalizedToken>
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, n = 20). Beta conidia hyaline, aseptate, filiform, few guttulate, hooked and mostly curved through 90-180°, tapering towards both ends, 16.0-22.7
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<normalizedToken originalValue="×">x</normalizedToken>
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1.0-1.5
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<normalizedToken originalValue="µm">µm</normalizedToken>
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(mean = 20.4
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<normalizedToken originalValue="×">x</normalizedToken>
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1.2
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<normalizedToken originalValue="μm">μm</normalizedToken>
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, n = 20). Gamma conidia and sexual morph not observed.
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="65" type="description">
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<paragraph pageId="0" pageNumber="65">Culture characteristics.</paragraph>
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<paragraph pageId="0" pageNumber="65">Pure culture was isolated by subbing hyphal tips growing from surface sterilized plant material. Colonies on PDA cover the Petri dish diameter after incubation for 15 days in dark conditions at 25 °C. Aerial mycelium white, cottony, feathery, with concentric zonation, white on surface side, pale brown to black on reverse side.</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="65" type="additional specimen examined">
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<paragraph pageId="0" pageNumber="65">Additional specimen examined.</paragraph>
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<paragraph pageId="0" pageNumber="65">
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China, Yunnan Province: Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, on infected leaves of
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<taxonomicName class="Dicotyledoneae" family="Theaceae" genus="Camellia" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="Camellia sinensis" order="Guttiferales" pageId="0" pageNumber="65" phylum="Angiospermae" rank="species" species="sinensis">
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<emphasis italics="true" pageId="0" pageNumber="65">Camellia sinensis</emphasis>
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</taxonomicName>
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. 19 April 2019, S.T. Huang, HSAUP194.102 paratype; living culture SAUCC194.102.
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="65" type="notes">
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<paragraph pageId="0" pageNumber="65">Notes.</paragraph>
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<paragraph pageId="0" pageNumber="65">
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This new species is proposed as the molecular data showed it forms a distinct clade with high support (ML/BI=98/1) and it appears most closely related to
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<taxonomicName lsidName="D. subellipicola" pageId="0" pageNumber="65" rank="species" species="subellipicola">
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<emphasis italics="true" pageId="0" pageNumber="65">D. subellipicola</emphasis>
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</taxonomicName>
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.
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<taxonomicName authorityName="S. T. Huang, J. W. Xia, X. G. Zhang & Z. Li" authorityYear="2021" class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe heterostemmatis" order="Diaporthales" pageId="0" pageNumber="65" phylum="Ascomycota" rank="species" species="heterostemmatis">
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<emphasis italics="true" pageId="0" pageNumber="65">Diaporthe heterostemmatis</emphasis>
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</taxonomicName>
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can be distinguished from
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<taxonomicName lsidName="D. subellipicola" pageId="0" pageNumber="65" rank="species" species="subellipicola">
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<emphasis italics="true" pageId="0" pageNumber="65">D. subellipicola</emphasis>
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</taxonomicName>
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by 57 nucleotides in concatenated alignment, in which 8 were distinct in the ITS region, 28 in the
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<emphasis italics="true" pageId="0" pageNumber="65">TUB</emphasis>
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region and 21 in the
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<emphasis italics="true" pageId="0" pageNumber="65">TEF</emphasis>
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region. Morphologically,
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<taxonomicName lsidName="D. subellipicola" pageId="0" pageNumber="65" rank="species" species="subellipicola">
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<emphasis italics="true" pageId="0" pageNumber="65">D. subellipicola</emphasis>
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</taxonomicName>
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was observed only on the basis of the sexual morph and culture characteristics (
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<bibRefCitation DOI="https://doi.org/10.5943/mycosphere/9/2/8" author="Hyde, KD" journalOrPublisher="Mycosphere" pageId="0" pageNumber="65" pagination="271 - 430" refId="B32" refString="Hyde, KD, Chaiwan, N, Norphanphoun, C, Boonmee, S, Camporesi, E, Chethana, KWT, Dayarathne, MC, de Silva, IN, Dissanayake, AJ, Ekanayaka, AH, 2018. Mycosphere notes 169-224. Mycosphere 9 (2): 271 - 430, DOI: https://doi.org/10.5943/mycosphere/9/2/8" title="Mycosphere notes 169 - 224." url="https://doi.org/10.5943/mycosphere/9/2/8" volume="9" year="2018">Hyde et al. 2018</bibRefCitation>
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).
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</paragraph>
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</subSubSection>
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</treatment>
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</document> |