treatments-xml/data/03/F9/87/03F987BDFFE9FFC7904DF9BA7C4E5BA1.xml

<document ID-DOI="10.1645/21-44" ID-GBIF-Dataset="72cc282b-8673-44f0-b6bd-78e7afbdf41c" ID-Zenodo-Dep="7093841" approvedBy="tatiana" checkinTime="1663610227247" checkinUser="tatiana" docAuthor="Jacobson, Elliott R., Ossiboff, Robert J., Paquet-Durand, Isabelle, Childress, April L., Barrett, Heather, Marlin, Jacob, McAllister, Chris T. &amp; Walden, Heather D. S." docDate="2022" docId="03F987BDFFE9FFC7904DF9BA7C4E5BA1" docLanguage="en" docName="JParasitol.108.1.93-99.pdf" docOrigin="Journal of Parasitology 108 (1)" docSource="https://doi.org/10.1645/21-44" docTitle="Eimeria grayi Jacobson &amp; Ossiboff &amp; Paquet-Durand &amp; Childress &amp; Barrett &amp; Marlin &amp; McAllister &amp; Walden 2022, n. sp." docType="treatment" docVersion="3" lastPageNumber="96" masterDocId="FFC0FFC5FFEAFFC39460FF9378435F42" masterDocTitle="A New Coccidian (Apicomplexa: Eimeriidae) in the Critically Endangered Central American River Turtle (Dermatemys Mawii) in Belize" masterLastPageNumber="99" masterPageNumber="92" pageNumber="95" updateTime="1663612848117" updateUser="ExternalLinkService" zenodo-license-document="CC-BY-NC-ND-4.0">
<mods:mods xmlns:mods="http://www.loc.gov/mods/v3">
<mods:titleInfo>
<mods:title>A New Coccidian (Apicomplexa: Eimeriidae) in the Critically Endangered Central American River Turtle (Dermatemys Mawii) in Belize</mods:title>
</mods:titleInfo>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Jacobson, Elliott R.</mods:namePart>
<mods:affiliation>College of Veterinary Medicine, University of Florida, Gainesville, Florida 32610</mods:affiliation>
<mods:nameIdentifier type="email">jacobsone@ufl.edu</mods:nameIdentifier>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Ossiboff, Robert J.</mods:namePart>
<mods:affiliation>College of Veterinary Medicine, University of Florida, Gainesville, Florida 32610</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Paquet-Durand, Isabelle</mods:namePart>
<mods:affiliation>Belize Wildlife &amp; Referral Clinic, San Ignacio, Cayo, Belize, Central America</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Childress, April L.</mods:namePart>
<mods:affiliation>College of Veterinary Medicine, University of Florida, Gainesville, Florida 32610</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Barrett, Heather</mods:namePart>
<mods:affiliation>Belize Foundation for Research and Environmental Education, Mile Marker 58, Southern Highway, Toledo, Belize, Central America</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Marlin, Jacob</mods:namePart>
<mods:affiliation>Belize Foundation for Research and Environmental Education, Mile Marker 58, Southern Highway, Toledo, Belize, Central America</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>McAllister, Chris T.</mods:namePart>
<mods:affiliation>Science and Mathematics Division, Eastern Oklahoma State College, Idabel, Oklahoma 74745.</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Walden, Heather D. S.</mods:namePart>
<mods:affiliation>College of Veterinary Medicine, University of Florida, Gainesville, Florida 32610</mods:affiliation>
</mods:name>
<mods:typeOfResource>text</mods:typeOfResource>
<mods:relatedItem type="host">
<mods:titleInfo>
<mods:title>Journal of Parasitology</mods:title>
</mods:titleInfo>
<mods:part>
<mods:date>2022</mods:date>
<mods:detail type="pubDate">
<mods:number>2022-02-22</mods:number>
</mods:detail>
<mods:detail type="volume">
<mods:number>108</mods:number>
</mods:detail>
<mods:detail type="issue">
<mods:number>1</mods:number>
</mods:detail>
<mods:extent unit="page">
<mods:start>92</mods:start>
<mods:end>99</mods:end>
</mods:extent>
</mods:part>
</mods:relatedItem>
<mods:location>
<mods:url>https://doi.org/10.1645/21-44</mods:url>
</mods:location>
<mods:classification>journal article</mods:classification>
<mods:identifier type="DOI">10.1645/21-44</mods:identifier>
<mods:identifier type="GBIF-Dataset">72cc282b-8673-44f0-b6bd-78e7afbdf41c</mods:identifier>
<mods:identifier type="Zenodo-Dep">7093841</mods:identifier>
</mods:mods>
<treatment ID-DOI="http://doi.org/10.5281/zenodo.7093843" ID-Zenodo-Dep="7093843" LSID="urn:lsid:plazi:treatment:03F987BDFFE9FFC7904DF9BA7C4E5BA1" httpUri="http://treatment.plazi.org/id/03F987BDFFE9FFC7904DF9BA7C4E5BA1" lastPageId="4" lastPageNumber="96" pageId="3" pageNumber="95">
<subSubSection box="[1069,1291,1577,1599]" pageId="3" pageNumber="95" type="nomenclature">
<paragraph blockId="3.[1069,1291,1577,1630]" box="[1069,1291,1577,1599]" pageId="3" pageNumber="95">
<heading bold="true" box="[1069,1291,1577,1599]" centered="true" fontSize="9" level="3" pageId="3" pageNumber="95" reason="4">
<emphasis bold="true" box="[1069,1291,1577,1599]" pageId="3" pageNumber="95">
<taxonomicName authority="Jacobson &amp; Ossiboff &amp; Paquet-Durand &amp; Childress &amp; Barrett &amp; Marlin &amp; McAllister &amp; Walden, 2022" authorityName="Jacobson &amp; Ossiboff &amp; Paquet-Durand &amp; Childress &amp; Barrett &amp; Marlin &amp; McAllister &amp; Walden" authorityYear="2022" box="[1069,1219,1577,1599]" family="Eimeriidae" genus="Eimeria" kingdom="Chromista" order="Eucoccidiida" pageId="3" pageNumber="95" phylum="Miozoa" rank="species" species="grayi" status="n. sp.">
<emphasis bold="true" box="[1069,1219,1577,1599]" italics="true" pageId="3" pageNumber="95">Eimeria grayi</emphasis>
</taxonomicName>
<taxonomicNameLabel box="[1228,1291,1577,1599]" pageId="3" pageNumber="95" rank="species">n. sp.</taxonomicNameLabel>
</emphasis>
</heading>
</paragraph>
</subSubSection>
<subSubSection lastPageId="4" lastPageNumber="96" pageId="3" pageNumber="95" type="description">
<paragraph blockId="3.[1069,1291,1577,1630]" box="[1128,1232,1608,1630]" pageId="3" pageNumber="95">
<heading box="[1128,1232,1608,1630]" centered="true" fontSize="9" level="5" pageId="3" pageNumber="95" reason="4">
(
<figureCitation box="[1133,1227,1608,1630]" captionStart-0="Figure 2" captionStart-1="Figure 3" captionStart-2="Figure 4" captionStart-3="Figure 5" captionStartId-0="3.[168,225,763,782]" captionStartId-1="3.[168,225,1888,1907]" captionStartId-2="3.[869,926,1166,1185]" captionStartId-3="4.[141,198,886,905]" captionTargetBox-0="[146,812,202,736]" captionTargetBox-1="[146,812,1278,1861]" captionTargetBox-2="[847,1513,209,1139]" captionTargetBox-3="[120,785,203,859]" captionTargetId-0="figure-270@3.[146,812,202,736]" captionTargetId-1="figure-293@3.[146,812,1278,1861]" captionTargetId-2="figure-329@3.[847,1513,209,1139]" captionTargetId-3="figure-776@4.[119,785,202,859]" captionTargetPageId-0="3" captionTargetPageId-1="3" captionTargetPageId-2="3" captionTargetPageId-3="4" captionText-0="Figure 2. Light microscopic photomicrograph of sporulated oocyst of Eimeria grayi n. sp. Oocyst showing 3 surface projections (arrows) at 1 pole." captionText-1="Figure 3. Light microscopic photomicrograph of sporulated oocyst of Eimeria grayi n. sp. Oocyst showing 2 of 3 surface projections (arrows) at 1 pole and a broader-based projection (arrowhead) from the opposite pole." captionText-2="Figure 4. Light microscopic photomicrograph of sporulated oocyst of Eimeria grayi n. sp. Oocyst showing 2 of 3 surface projections (arrows) at 1 pole, a broader-based projection (arrowhead) from the opposite pole, as well as the location of the sporocyst residuum (SR)." captionText-3="Figure 5. Composite line drawing of a sporulated mitra-shaped oocyst of Eimeria grayi n. sp." figureDoi-0="http://doi.org/10.5281/zenodo.7093850" figureDoi-1="http://doi.org/10.5281/zenodo.7093854" figureDoi-2="http://doi.org/10.5281/zenodo.7093852" figureDoi-3="http://doi.org/10.5281/zenodo.7093856" httpUri-0="https://zenodo.org/record/7093850/files/figure.png" httpUri-1="https://zenodo.org/record/7093854/files/figure.png" httpUri-2="https://zenodo.org/record/7093852/files/figure.png" httpUri-3="https://zenodo.org/record/7093856/files/figure.png" pageId="3" pageNumber="95">Figs. 2–5</figureCitation>
)
</heading>
</paragraph>
<paragraph blockId="3.[845,1515,1665,1956]" pageId="3" pageNumber="95">
<emphasis box="[869,1231,1666,1687]" italics="true" pageId="3" pageNumber="95">Description of sporulated oocyst:</emphasis>
Oocyst shape (
<emphasis box="[1418,1430,1666,1687]" italics="true" pageId="3" pageNumber="95">n</emphasis>
<emphasis bold="true" box="[1444,1462,1665,1686]" pageId="3" pageNumber="95">-</emphasis>
15): ellipsoidal; smooth, thin-walled; L 
<emphasis bold="true" box="[1226,1246,1695,1717]" pageId="3" pageNumber="95">x</emphasis>
W: 30.4 (26–35) 
<emphasis bold="true" box="[1442,1462,1695,1717]" pageId="3" pageNumber="95">x</emphasis>
15.2 (13–17), L/W: 2.0 (1.7–2.5); M, OR, PG: all absent. Distinctive feature of oocyst: 3 conical projections (2.1–3.5 long) present on 1 end, with the oocyst wall forming a broadly pointed end on the opposite pole.
</paragraph>
<paragraph blockId="3.[845,1515,1665,1956]" lastBlockId="4.[117,787,973,1084]" lastPageId="4" lastPageNumber="96" pageId="3" pageNumber="95">
<emphasis box="[869,1298,1845,1866]" italics="true" pageId="3" pageNumber="95">Description of sporocyst and sporozoites:</emphasis>
SP: (
<emphasis box="[1364,1376,1845,1866]" italics="true" pageId="3" pageNumber="95">n</emphasis>
<emphasis bold="true" box="[1385,1403,1845,1866]" pageId="3" pageNumber="95">-</emphasis>
4); shape: elongate–ellipsoidal, with a smooth single-layered wall; L 
<emphasis bold="true" box="[1457,1477,1874,1896]" pageId="3" pageNumber="95">x</emphasis>
W: 13.7 
<emphasis bold="true" box="[898,918,1904,1926]" pageId="3" pageNumber="95">x</emphasis>
7.5 (12–19 
<emphasis bold="true" box="[1050,1070,1904,1926]" pageId="3" pageNumber="95">x</emphasis>
6–12), L/W 1.8 (1.7–2.2); SR: sporocyst residuum, coarsely granular, and scattered; SZ: banana-shaped, 14.2 
<emphasis bold="true" box="[167,187,973,995]" pageId="4" pageNumber="96">x</emphasis>
3.6 (14–15 
<emphasis bold="true" box="[309,329,973,995]" pageId="4" pageNumber="96">x</emphasis>
3–4) in situ; N visible, slightly posterior to midpoint of SZ. Distinctive feature of SP: slightly pointed at 1 end with a distinct SB, SR of only a few globules, and a SRB in each SZ.
</paragraph>
</subSubSection>
<caption ID-DOI="http://doi.org/10.5281/zenodo.7093854" ID-Zenodo-Dep="7093854" httpUri="https://zenodo.org/record/7093854/files/figure.png" pageId="3" pageNumber="95" startId="3.[168,225,1888,1907]" targetBox="[146,812,1278,1861]" targetPageId="3">
<paragraph blockId="3.[144,814,1888,1955]" pageId="3" pageNumber="95">
<emphasis bold="true" box="[168,244,1888,1907]" pageId="3" pageNumber="95">Figure 3</emphasis>
. Light microscopic photomicrograph of sporulated oocyst of 
<taxonomicName authority="Jacobson &amp; Ossiboff &amp; Paquet-Durand &amp; Childress &amp; Barrett &amp; Marlin &amp; McAllister &amp; Walden, 2022" authorityName="Jacobson &amp; Ossiboff &amp; Paquet-Durand &amp; Childress &amp; Barrett &amp; Marlin &amp; McAllister &amp; Walden" authorityYear="2022" box="[144,264,1912,1931]" family="Eimeriidae" genus="Eimeria" kingdom="Chromista" order="Eucoccidiida" pageId="3" pageNumber="95" phylum="Miozoa" rank="species" species="grayi" status="n. sp.">
<emphasis box="[144,264,1912,1931]" italics="true" pageId="3" pageNumber="95">Eimeria grayi</emphasis>
</taxonomicName>
<taxonomicNameLabel box="[269,318,1912,1931]" pageId="3" pageNumber="95" rank="species">n. sp.</taxonomicNameLabel>
Oocyst showing 2 of 3 surface projections (arrows) at 1 pole and a broader-based projection (arrowhead) from the opposite pole.
</paragraph>
</caption>
<caption ID-DOI="http://doi.org/10.5281/zenodo.7093856" ID-Zenodo-Dep="7093856" httpUri="https://zenodo.org/record/7093856/files/figure.png" pageId="4" pageNumber="96" startId="4.[141,198,886,905]" targetBox="[120,785,203,859]" targetPageId="4">
<paragraph blockId="4.[117,787,886,929]" pageId="4" pageNumber="96">
<emphasis bold="true" box="[141,220,886,905]" pageId="4" pageNumber="96">Figure 5.</emphasis>
Composite line drawing of a sporulated mitra-shaped oocyst of 
<taxonomicName authority="Jacobson &amp; Ossiboff &amp; Paquet-Durand &amp; Childress &amp; Barrett &amp; Marlin &amp; McAllister &amp; Walden, 2022" authorityName="Jacobson &amp; Ossiboff &amp; Paquet-Durand &amp; Childress &amp; Barrett &amp; Marlin &amp; McAllister &amp; Walden" authorityYear="2022" box="[143,266,910,929]" family="Eimeriidae" genus="Eimeria" kingdom="Chromista" order="Eucoccidiida" pageId="4" pageNumber="96" phylum="Miozoa" rank="species" species="grayi" status="n. sp.">
<emphasis box="[143,266,910,929]" italics="true" pageId="4" pageNumber="96">Eimeria grayi</emphasis>
</taxonomicName>
<taxonomicNameLabel box="[273,324,910,929]" pageId="4" pageNumber="96" rank="species">n. sp.</taxonomicNameLabel>
</paragraph>
</caption>
<subSubSection pageId="4" pageNumber="96" type="discussion">
<paragraph blockId="4.[117,356,1120,1142]" box="[117,356,1120,1142]" pageId="4" pageNumber="96">
<heading bold="true" box="[117,356,1120,1142]" fontSize="9" level="4" pageId="4" pageNumber="96" reason="2">
<emphasis bold="true" box="[117,356,1120,1142]" pageId="4" pageNumber="96">Taxonomic summary</emphasis>
</heading>
</paragraph>
<paragraph blockId="4.[117,787,1166,1956]" pageId="4" pageNumber="96">
<emphasis box="[141,252,1167,1188]" italics="true" pageId="4" pageNumber="96">Type host:</emphasis>
Central American river turtle, 
<taxonomicName authority="Gray, 1847" authorityName="Gray" authorityYear="1847" class="Reptilia" family="Dermatemydidae" genus="Dermatemys" kingdom="Animalia" order="Testudines" pageId="4" pageNumber="96" phylum="Chordata" rank="species" species="mawii">
<emphasis box="[590,786,1167,1188]" italics="true" pageId="4" pageNumber="96">Dermatemys mawii</emphasis>
Gray, 1847
</taxonomicName>
(
<taxonomicName box="[249,363,1196,1218]" class="Reptilia" kingdom="Animalia" order="Testudines" pageId="4" pageNumber="96" phylum="Chordata" rank="order">Testudines</taxonomicName>
: 
<taxonomicName box="[375,556,1196,1218]" class="Reptilia" family="Dermatemydidae" kingdom="Animalia" order="Testudines" pageId="4" pageNumber="96" phylum="Chordata" rank="family">Dermatemydidae</taxonomicName>
). Collected 11 August and 11 November 2018. Host photovouchers: University of Florida (UF) 191862-191866.
</paragraph>
</subSubSection>
<subSubSection pageId="4" pageNumber="96" type="materials_examined">
<paragraph blockId="4.[117,787,1166,1956]" pageId="4" pageNumber="96">
<emphasis box="[141,386,1285,1306]" italics="true" pageId="4" pageNumber="96">Type and only locality:</emphasis>
<materialsCitation collectionCode="HWML" country="Belize" latitude="16.514361" location="Belize Foundation for Research and Environmental Education" longitude="-88.569859" pageId="4" pageNumber="96" specimenCode="HWML 206401" specimenCount="1" typeStatus="holotype">
<location LSID="urn:lsid:plazi:treatment:03F987BDFFE9FFC7904DF9BA7C4E5BA1:8E8F6070FFEEFFC795EDFA9779C75A7A" country="Belize" name="Belize Foundation for Research and Environmental Education" pageId="4" pageNumber="96">Belize Foundation for Research and Environmental Education</location>
, 
<location LSID="urn:lsid:plazi:treatment:03F987BDFFE9FFC7904DF9BA7C4E5BA1:8E8F6070FFEEFFC795F1FAB17A785A7A" box="[401,571,1314,1336]" country="Belize" name="Mile Marker 58" pageId="4" pageNumber="96">Mile Marker 58</location>
, 
<location LSID="urn:lsid:plazi:treatment:03F987BDFFE9FFC7904DF9BA7C4E5BA1:8E8F6070FFEEFFC79629FAB17B4C5A7A" box="[585,783,1314,1336]" country="Belize" name="Southern Highway" pageId="4" pageNumber="96">Southern Highway</location>
, 
<collectingCountry box="[117,180,1343,1365]" pageId="4" pageNumber="96">Belize</collectingCountry>
, 
<location LSID="urn:lsid:plazi:treatment:03F987BDFFE9FFC7904DF9BA7C4E5BA1:8E8F6070FFEEFFC794A0FAAC792F5A17" box="[192,364,1343,1365]" country="Belize" name="Central America" pageId="4" pageNumber="96">Central America</location>
(16 
<emphasis bold="true" box="[403,411,1343,1365]" pageId="4" pageNumber="96">°</emphasis>
33.326 
<emphasis bold="true" box="[478,483,1340,1355]" pageId="4" pageNumber="96">
<superScript attach="right" box="[478,483,1340,1355]" fontSize="6" pageId="4" pageNumber="96">'</superScript>
</emphasis>
N, 88 
<emphasis bold="true" box="[542,550,1343,1365]" pageId="4" pageNumber="96">°</emphasis>
42.423 
<emphasis bold="true" box="[618,623,1340,1355]" pageId="4" pageNumber="96">
<superScript attach="right" box="[618,623,1340,1355]" fontSize="6" pageId="4" pageNumber="96">'</superScript>
</emphasis>
W).
</materialsCitation>
</paragraph>
</subSubSection>
<subSubSection pageId="4" pageNumber="96" type="discussion">
<paragraph blockId="4.[117,787,1166,1956]" pageId="4" pageNumber="96">
<emphasis box="[141,260,1373,1394]" italics="true" pageId="4" pageNumber="96">Prevalence:</emphasis>
1/3 (33%) based on oocyst identification in samples; 3/3 (100%) based on PCR and sequencing of the 
<emphasis box="[748,786,1403,1424]" italics="true" pageId="4" pageNumber="96">18S</emphasis>
rRNA and 
<emphasis box="[237,277,1432,1453]" italics="true" pageId="4" pageNumber="96">ITS</emphasis>
genes.
</paragraph>
</subSubSection>
<subSubSection pageId="4" pageNumber="96" type="materials_examined">
<paragraph blockId="4.[117,787,1166,1956]" pageId="4" pageNumber="96">
<emphasis box="[141,351,1462,1483]" italics="true" pageId="4" pageNumber="96">Materials deposited:</emphasis>
Photosyntype of a sporulated oocyst is deposited in the Harold W. Manter Laboratory of Parasitology (HWML), University of Nebraska, as HWML 206401.
</paragraph>
</subSubSection>
<subSubSection pageId="4" pageNumber="96" type="discussion">
<paragraph blockId="4.[117,787,1166,1956]" pageId="4" pageNumber="96">
<emphasis box="[141,382,1551,1572]" italics="true" pageId="4" pageNumber="96">ZooBank registration:</emphasis>
urn:lsid:zoobank.org:act:B7C62C80- DF94-49F0-A73F-586C1FB2B578.
</paragraph>
</subSubSection>
<subSubSection pageId="4" pageNumber="96" type="etymology">
<paragraph blockId="4.[117,787,1166,1956]" pageId="4" pageNumber="96">
<emphasis box="[141,258,1610,1631]" italics="true" pageId="4" pageNumber="96">Etymology:</emphasis>
The specific epithet is named in honor of the British naturalist, John Edward Gray (1800–1875), who described the host in 1847.
</paragraph>
</subSubSection>
<subSubSection pageId="4" pageNumber="96" type="discussion">
<paragraph blockId="4.[117,787,1166,1956]" lastBlockId="4.[818,1488,202,492]" pageId="4" pageNumber="96">
<emphasis box="[141,335,1698,1719]" italics="true" pageId="4" pageNumber="96">Molecular biology:</emphasis>
All 3 fecal samples had similar content of the 
<emphasis box="[160,198,1728,1749]" italics="true" pageId="4" pageNumber="96">18S</emphasis>
rRNA and 
<emphasis box="[337,377,1728,1749]" italics="true" pageId="4" pageNumber="96">ITS</emphasis>
genes. After primer editing, a 347-bp segment of the 
<emphasis box="[282,321,1757,1778]" italics="true" pageId="4" pageNumber="96">18S</emphasis>
rRNA gene was determined for the hicatee coccidian (GenBank accession MW538581
<emphasis bold="true" box="[581,589,1787,1809]" pageId="4" pageNumber="96">)</emphasis>
. BLASTn results identified 99.4% sequence identity to several apicomplexan organisms, including 
<taxonomicName authorityName="Leger" authorityYear="1904" box="[339,455,1846,1867]" family="Eimeriidae" genus="Caryospora" kingdom="Chromista" order="Eucoccidiida" pageId="4" pageNumber="96" phylum="Miozoa" rank="genus">
<emphasis box="[339,455,1846,1867]" italics="true" pageId="4" pageNumber="96">Caryospora</emphasis>
</taxonomicName>
-like sp. US3 (MN450817) from a green sea turtle (
<taxonomicName baseAuthorityName="Linnaeus" baseAuthorityYear="1758" box="[350,523,1875,1896]" class="Reptilia" family="Cheloniidae" genus="Chelonia" kingdom="Animalia" order="Testudines" pageId="4" pageNumber="96" phylum="Chordata" rank="species" species="mydas">
<emphasis box="[350,523,1875,1896]" italics="true" pageId="4" pageNumber="96">Chelonia mydas</emphasis>
</taxonomicName>
), 
<taxonomicName box="[555,786,1875,1896]" family="Eimeriidae" genus="Caryospora" kingdom="Chromista" order="Eucoccidiida" pageId="4" pageNumber="96" phylum="Miozoa" rank="species" species="cheloniae">
<emphasis box="[555,786,1875,1896]" italics="true" pageId="4" pageNumber="96">Caryospora cheloniae</emphasis>
</taxonomicName>
genotype 1 (KT361639) from 
<taxonomicName baseAuthorityName="Linnaeus" baseAuthorityYear="1758" box="[448,560,1905,1926]" class="Reptilia" family="Cheloniidae" genus="Chelonia" kingdom="Animalia" order="Testudines" pageId="4" pageNumber="96" phylum="Chordata" rank="species" species="mydas">
<emphasis box="[448,560,1905,1926]" italics="true" pageId="4" pageNumber="96">Ch. mydas</emphasis>
</taxonomicName>
, and multiple lizard 
<taxonomicName box="[117,283,1934,1956]" family="Lankesterellidae" genus="Schellackia" kingdom="Chromista" order="Eucoccidiida" pageId="4" pageNumber="96" phylum="Miozoa" rank="species" species="undetermined">
<emphasis box="[117,231,1934,1955]" italics="true" pageId="4" pageNumber="96">Schellackia</emphasis>
spp.
</taxonomicName>
The hicatee coccidian was 99.7% identical to a 347-bp segment of the 
<emphasis box="[1052,1090,203,224]" italics="true" pageId="4" pageNumber="96">18S</emphasis>
rRNA gene of the reference sample of 
<taxonomicName box="[818,930,232,253]" family="Eimeriidae" genus="Eimeria" kingdom="Chromista" order="Eucoccidiida" pageId="4" pageNumber="96" phylum="Miozoa" rank="species" species="mitraria">
<emphasis box="[818,930,232,253]" italics="true" pageId="4" pageNumber="96">E. mitraria</emphasis>
</taxonomicName>
(GenBank accession MW538549). An 841-bp segment of the ITS region was determined for the hicatee coccidian and reference sample of 
<taxonomicName box="[1025,1137,292,313]" family="Eimeriidae" genus="Eimeria" kingdom="Chromista" order="Eucoccidiida" pageId="4" pageNumber="96" phylum="Miozoa" rank="species" species="mitraria">
<emphasis box="[1025,1137,292,313]" italics="true" pageId="4" pageNumber="96">E. mitraria</emphasis>
</taxonomicName>
(GenBank accessions MW538550 and MW538533, respectively), with 75.2% sequence identity to each other. Bayesian phylogenetic analysis placed the hicatee coccidian in a clade with 
<taxonomicName box="[1099,1258,381,402]" family="Eimeriidae" genus="Eimeria" kingdom="Chromista" order="Eucoccidiida" pageId="4" pageNumber="96" phylum="Miozoa" rank="species" species="collieie">
<emphasis box="[1099,1258,381,402]" italics="true" pageId="4" pageNumber="96">Eimeria collieie</emphasis>
</taxonomicName>
, 
<emphasis italics="true" pageId="4" pageNumber="96">
<taxonomicName box="[1276,1394,381,402]" family="Eimeriidae" genus="Eimeria" kingdom="Chromista" order="Eucoccidiida" pageId="4" pageNumber="96" phylum="Miozoa" rank="species" species="mitraria">E. mitraria</taxonomicName>
, 
<taxonomicName family="Eimeriidae" genus="Eimeria" kingdom="Chromista" order="Eucoccidiida" pageId="4" pageNumber="96" phylum="Miozoa" rank="species" species="arnyi">Eimeria arnyi</taxonomicName>
</emphasis>
, and 2 coccidians of green sea turtles (
<taxonomicName box="[1300,1438,411,432]" family="Eimeriidae" genus="Caryospora" kingdom="Chromista" order="Eucoccidiida" pageId="4" pageNumber="96" phylum="Miozoa" rank="species" species="cheloniae">
<emphasis box="[1300,1438,411,432]" italics="true" pageId="4" pageNumber="96">Ca. cheloniae</emphasis>
</taxonomicName>
and Caryospora-like sp. US3) with a posterior probability of 0.89 (
<figureCitation box="[826,889,470,492]" captionStart="Figure 6" captionStartId="5.[168,225,1542,1561]" captionTargetBox="[145,1515,202,1515]" captionTargetId="figure-201@5.[145,1515,202,1515]" captionTargetPageId="5" captionText="Figure 6. Evolutionary relationships of Eimeria grayi n. sp. inferred by Bayesian analysis of partial 18S rRNA sequences. Posterior probabilities are shown at branch points. Toxoplasma gondii (EF472967) was selected as the outgroup. Eimeria grayi n. sp. is bolded and underlined." figureDoi="http://doi.org/10.5281/zenodo.7093858" httpUri="https://zenodo.org/record/7093858/files/figure.png" pageId="4" pageNumber="96">Fig. 6</figureCitation>
).
</paragraph>
<paragraph blockId="4.[818,918,528,550]" box="[818,918,528,550]" pageId="4" pageNumber="96">
<heading bold="true" box="[818,918,528,550]" fontSize="9" level="4" pageId="4" pageNumber="96" reason="2">
<emphasis bold="true" box="[818,918,528,550]" pageId="4" pageNumber="96">Remarks</emphasis>
</heading>
</paragraph>
<paragraph blockId="4.[818,1493,575,1251]" pageId="4" pageNumber="96">
The oocysts being passed by the hicatee possessed multiple conical projections with 3 projecting from 1 pole and a broaderbased projection from the opposite pole (
<figureCitation box="[1235,1331,634,656]" captionStart-0="Figure 2" captionStart-1="Figure 3" captionStart-2="Figure 4" captionStart-3="Figure 5" captionStartId-0="3.[168,225,763,782]" captionStartId-1="3.[168,225,1888,1907]" captionStartId-2="3.[869,926,1166,1185]" captionStartId-3="4.[141,198,886,905]" captionTargetBox-0="[146,812,202,736]" captionTargetBox-1="[146,812,1278,1861]" captionTargetBox-2="[847,1513,209,1139]" captionTargetBox-3="[120,785,203,859]" captionTargetId-0="figure-270@3.[146,812,202,736]" captionTargetId-1="figure-293@3.[146,812,1278,1861]" captionTargetId-2="figure-329@3.[847,1513,209,1139]" captionTargetId-3="figure-776@4.[119,785,202,859]" captionTargetPageId-0="3" captionTargetPageId-1="3" captionTargetPageId-2="3" captionTargetPageId-3="4" captionText-0="Figure 2. Light microscopic photomicrograph of sporulated oocyst of Eimeria grayi n. sp. Oocyst showing 3 surface projections (arrows) at 1 pole." captionText-1="Figure 3. Light microscopic photomicrograph of sporulated oocyst of Eimeria grayi n. sp. Oocyst showing 2 of 3 surface projections (arrows) at 1 pole and a broader-based projection (arrowhead) from the opposite pole." captionText-2="Figure 4. Light microscopic photomicrograph of sporulated oocyst of Eimeria grayi n. sp. Oocyst showing 2 of 3 surface projections (arrows) at 1 pole, a broader-based projection (arrowhead) from the opposite pole, as well as the location of the sporocyst residuum (SR)." captionText-3="Figure 5. Composite line drawing of a sporulated mitra-shaped oocyst of Eimeria grayi n. sp." figureDoi-0="http://doi.org/10.5281/zenodo.7093850" figureDoi-1="http://doi.org/10.5281/zenodo.7093854" figureDoi-2="http://doi.org/10.5281/zenodo.7093852" figureDoi-3="http://doi.org/10.5281/zenodo.7093856" httpUri-0="https://zenodo.org/record/7093850/files/figure.png" httpUri-1="https://zenodo.org/record/7093854/files/figure.png" httpUri-2="https://zenodo.org/record/7093852/files/figure.png" httpUri-3="https://zenodo.org/record/7093856/files/figure.png" pageId="4" pageNumber="96">Figs. 2–5</figureCitation>
). To date, the 7 previously described oocysts of 
<taxonomicName box="[1179,1288,664,686]" class="Reptilia" kingdom="Animalia" order="Testudines" pageId="4" pageNumber="96" phylum="Chordata" rank="order">Testudines</taxonomicName>
having mitra-like conical projections or irregular surfaces from at least 1 end of their oocyst are (1) 
<taxonomicName box="[1044,1260,724,745]" family="Eimeriidae" genus="Eimeria" kingdom="Chromista" order="Eucoccidiida" pageId="4" pageNumber="96" phylum="Miozoa" rank="species" species="amazonensis">
<emphasis box="[1044,1260,724,745]" italics="true" pageId="4" pageNumber="96">Eimeria amazonensis</emphasis>
</taxonomicName>
from the red-footed tortoise, 
<taxonomicName authorityName="Fitzinger" authorityYear="1835" box="[908,1024,754,775]" class="Reptilia" family="Testudinidae" genus="Chelonoidis" kingdom="Animalia" order="Testudines" pageId="4" pageNumber="96" phylum="Chordata" rank="genus">
<emphasis box="[908,1024,754,775]" italics="true" pageId="4" pageNumber="96">Chelonoidis</emphasis>
</taxonomicName>
(formerly 
<taxonomicName authorityName="Fitzinger" authorityYear="1835" box="[1132,1245,754,775]" class="Reptilia" family="Testudinidae" genus="Geochelone" kingdom="Animalia" order="Testudines" pageId="4" pageNumber="96" phylum="Chordata" rank="genus">
<emphasis box="[1132,1245,754,775]" italics="true" pageId="4" pageNumber="96">Geochelone</emphasis>
</taxonomicName>
) 
<emphasis box="[1259,1366,754,775]" italics="true" pageId="4" pageNumber="96">carbonaria</emphasis>
from Brazil (
<bibRefCitation author="LAINSON, R. &amp; F. M. M. DA SILVA &amp; C. M. FRANCO &amp; M. C. DE SOUZA" box="[825,1065,783,805]" journalOrPublisher="Parasite" pageId="4" pageNumber="96" pagination="531 - 538" part="15" refId="ref5719" refString="LAINSON, R., F. M. M. DA SILVA, C. M. FRANCO, AND M. C. DE SOUZA. 2008. New species of Eimeria and Isospora (Protozoa: Eimeriidae) in Geochelone spp. (Chelonia: Testudinidae) from Amazonian Brazil. Parasite 15: 531 - 538." title="New species of Eimeria and Isospora (Protozoa: Eimeriidae) in Geochelone spp. (Chelonia: Testudinidae) from Amazonian Brazil" type="journal article" year="2008">Lainson et al., 2008</bibRefCitation>
); (2) 
<taxonomicName box="[1137,1372,783,804]" family="Eimeriidae" genus="Eimeria" kingdom="Chromista" order="Eucoccidiida" pageId="4" pageNumber="96" phylum="Miozoa" rank="species" species="hynekprokopi">
<emphasis box="[1137,1372,783,804]" italics="true" pageId="4" pageNumber="96">Eimeria hynekprokopi</emphasis>
</taxonomicName>
from the Indochinese box turtle, 
<taxonomicName authorityName="Bourret" authorityYear="1939" box="[1064,1251,813,834]" class="Reptilia" family="Geoemydidae" genus="Cuora" kingdom="Animalia" order="Testudines" pageId="4" pageNumber="96" phylum="Chordata" rank="species" species="galbinifrons">
<emphasis box="[1064,1251,813,834]" italics="true" pageId="4" pageNumber="96">Cuora galbinifrons</emphasis>
</taxonomicName>
from Vietnam (Široký and Modrý, 2010); (3) 
<taxonomicName box="[1053,1215,843,864]" family="Eimeriidae" genus="Eimeria" kingdom="Chromista" order="Eucoccidiida" pageId="4" pageNumber="96" phylum="Miozoa" rank="species" species="iversoni">
<emphasis box="[1053,1215,843,864]" italics="true" pageId="4" pageNumber="96">Eimeria iversoni</emphasis>
</taxonomicName>
from a captive Galapagos tortoise, 
<taxonomicName box="[916,1074,872,894]" class="Reptilia" family="Testudinidae" genus="Chelonoidis" kingdom="Animalia" order="Testudines" pageId="4" pageNumber="96" phylum="Chordata" rank="species" species="undetermined">
<emphasis box="[916,1032,872,893]" italics="true" pageId="4" pageNumber="96">Chelonoidis</emphasis>
sp.
</taxonomicName>
(
<bibRefCitation author="MCALLISTER, C. T. &amp; D. W. DUSZYNSKI &amp; D. T. ROBERTS" box="[1093,1344,872,894]" journalOrPublisher="Journal of Parasitology" pageId="4" pageNumber="96" pagination="128 - 132" part="100" refId="ref5862" refString="MCALLISTER, C. T., D. W. DUSZYNSKI, AND D. T. ROBERTS. 2014. A new coccidian (Apicomplexa: Eimeridae) from Galapagos tortoise, Chelonoidis sp. (Tesudines: Testudinidae), from the Dallas Zoo. Journal of Parasitology 100: 128 - 132." title="A new coccidian (Apicomplexa: Eimeridae) from Galapagos tortoise, Chelonoidis sp. (Tesudines: Testudinidae), from the Dallas Zoo" type="journal article" year="2014">McAllister et al., 2014</bibRefCitation>
); (4) 
<taxonomicName family="Eimeriidae" genus="Eimeria" kingdom="Chromista" order="Eucoccidiida" pageId="4" pageNumber="96" phylum="Miozoa" rank="species" species="jirkamoraveci">
<emphasis italics="true" pageId="4" pageNumber="96">Eimeria jirkamoraveci</emphasis>
</taxonomicName>
in the Neotropical chelid turtle 
<taxonomicName authorityName="Gray" authorityYear="1873" box="[1346,1488,902,923]" class="Reptilia" family="Chelidae" genus="Mesoclemmys" kingdom="Animalia" order="Testudines" pageId="4" pageNumber="96" phylum="Chordata" rank="genus">
<emphasis box="[1346,1488,902,923]" italics="true" pageId="4" pageNumber="96">Mesoclemmys</emphasis>
</taxonomicName>
(previously 
<taxonomicName authorityName="Stejneger" authorityYear="1909" box="[943,1072,932,953]" class="Reptilia" family="Chelidae" genus="Batrachemys" kingdom="Animalia" order="Testudines" pageId="4" pageNumber="96" phylum="Chordata" rank="genus">
<emphasis box="[943,1072,932,953]" italics="true" pageId="4" pageNumber="96">Batrachemys</emphasis>
</taxonomicName>
) 
<taxonomicName authorityName="Mccord, Josephouni &amp; Lamar" authorityYear="2001" box="[1092,1216,932,953]" class="Reptilia" family="Chelidae" genus="Batrachemys" kingdom="Animalia" order="Testudines" pageId="4" pageNumber="96" phylum="Chordata" rank="species" species="heliostemma">
<emphasis box="[1092,1216,932,953]" italics="true" pageId="4" pageNumber="96">heliostemma</emphasis>
</taxonomicName>
(Široký et al., 2006); (5) 
<taxonomicName box="[818,934,962,983]" family="Eimeriidae" genus="Eimeria" kingdom="Chromista" order="Eucoccidiida" pageId="4" pageNumber="96" phylum="Miozoa" rank="species" species="mitraria">
<emphasis box="[818,934,962,983]" italics="true" pageId="4" pageNumber="96">E. mitraria</emphasis>
</taxonomicName>
originally described from the Asian Chinese threekeeled pond turtle, 
<taxonomicName authorityName="Ritgen" authorityYear="1828" box="[1027,1134,991,1012]" class="Reptilia" family="Geoemydidae" genus="Mauremys" kingdom="Animalia" order="Testudines" pageId="4" pageNumber="96" phylum="Chordata" rank="genus">
<emphasis box="[1027,1134,991,1012]" italics="true" pageId="4" pageNumber="96">Mauremys</emphasis>
</taxonomicName>
(formerly 
<taxonomicName authorityName="Smith" authorityYear="1931" box="[1250,1348,991,1012]" class="Reptilia" family="Geoemydidae" genus="Chinemys" kingdom="Animalia" order="Testudines" pageId="4" pageNumber="96" phylum="Chordata" rank="genus">
<emphasis box="[1250,1348,991,1012]" italics="true" pageId="4" pageNumber="96">Chinemys</emphasis>
</taxonomicName>
) 
<emphasis box="[1366,1439,991,1012]" italics="true" pageId="4" pageNumber="96">reevesii</emphasis>
and subsequently described in multiple species of aquatic turtles on 3 continents (
<bibRefCitation author="LAVERAN, A. &amp; F. MESNIL" box="[940,1219,1051,1073]" journalOrPublisher="Comptes Rendus de l'Acade mie des Sciences" pageId="4" pageNumber="96" pagination="609 - 614" part="134" refId="ref5780" refString="LAVERAN, A., AND F. MESNIL. 1902. Sur quelques protozoaires parasites d'une tome d'Asie (Damonia reevesii). Comptes Rendus de l'Acade mie des Sciences Series 3 134: 609 - 614." title="Sur quelques protozoaires parasites d'une tome d'Asie (Damonia reevesii)" type="journal article" year="1902">Laveran and Mesnil, 1902</bibRefCitation>
; 
<bibRefCitation author="DUSZYNSKI, D. W. &amp; J. MORROW" bookContentInfo="210 p." journalOrPublisher="Elsevier Academic Press, Boston, Massachusetts" pageId="4" pageNumber="96" refId="ref5385" refString="DUSZYNSKI, D. W., AND J. MORROW. 2014. The Biology and Identification of the Coccidia (Apicomplexa) of Turtles of the World. Elsevier Academic Press, Boston, Massachusetts, 210 p." title="The Biology and Identification of the Coccidia (Apicomplexa) of Turtles of the World" type="book" year="2014">Duszynski and Morrow, 2014</bibRefCitation>
); (6) 
<taxonomicName authorityName="Yakimoff" authorityYear="1929" baseAuthorityName="Labbé" baseAuthorityYear="1893" box="[944,1110,1081,1102]" family="Eimeriidae" genus="Eimeria" kingdom="Chromista" order="Eucoccidiida" pageId="4" pageNumber="96" phylum="Miozoa" rank="species" species="motelo">
<emphasis box="[944,1110,1081,1102]" italics="true" pageId="4" pageNumber="96">Eimeria motelo</emphasis>
</taxonomicName>
from the yellow-footed tortoise, 
<taxonomicName authorityName="Fitzinger" authorityYear="1835" box="[818,934,1110,1131]" class="Reptilia" family="Testudinidae" genus="Chelonoidis" kingdom="Animalia" order="Testudines" pageId="4" pageNumber="96" phylum="Chordata" rank="genus">
<emphasis box="[818,934,1110,1131]" italics="true" pageId="4" pageNumber="96">Chelonoidis</emphasis>
</taxonomicName>
(formerly 
<taxonomicName authorityName="Fitzinger" authorityYear="1835" box="[1052,1165,1110,1131]" class="Reptilia" family="Testudinidae" genus="Geochelone" kingdom="Animalia" order="Testudines" pageId="4" pageNumber="96" phylum="Chordata" rank="genus">
<emphasis box="[1052,1165,1110,1131]" italics="true" pageId="4" pageNumber="96">Geochelone</emphasis>
</taxonomicName>
) 
<taxonomicName authorityName="Williams" authorityYear="1960" box="[1184,1294,1110,1131]" class="Reptilia" family="Testudinidae" genus="Geochelone" kingdom="Animalia" order="Testudines" pageId="4" pageNumber="96" phylum="Chordata" rank="species" species="denticulata">
<emphasis box="[1184,1294,1110,1131]" italics="true" pageId="4" pageNumber="96">denticulata</emphasis>
</taxonomicName>
, from Peru (Hurkováet al., 2000); and (7) 
<taxonomicName box="[1096,1253,1140,1161]" family="Eimeriidae" genus="Eimeria" kingdom="Chromista" order="Eucoccidiida" pageId="4" pageNumber="96" phylum="Miozoa" rank="species" species="stylosa">
<emphasis box="[1096,1253,1140,1161]" italics="true" pageId="4" pageNumber="96">Eimeria stylosa</emphasis>
</taxonomicName>
in the red-eared slider from Texas (
<bibRefCitation author="MCALLISTER, C. T. &amp; S. J. UPTON" box="[950,1247,1169,1191]" journalOrPublisher="Canadian Journal of Zoology" pageId="4" pageNumber="96" pagination="2459 - 2467" part="67" refId="ref5819" refString="MCALLISTER, C. T., AND S. J. UPTON. 1989. The coccidia (Apicomplexa: Eimeriidae) of Testudines, with descriptions of three new species. Canadian Journal of Zoology 67: 2459 - 2467." title="The coccidia (Apicomplexa: Eimeriidae) of Testudines, with descriptions of three new species" type="journal article" year="1989">McAllister and Upton, 1989</bibRefCitation>
). Overall, oocysts of 
<taxonomicName authorityName="Jacobson &amp; Ossiboff &amp; Paquet-Durand &amp; Childress &amp; Barrett &amp; Marlin &amp; McAllister &amp; Walden" authorityYear="2022" family="Eimeriidae" genus="Eimeria" kingdom="Chromista" order="Eucoccidiida" pageId="4" pageNumber="96" phylum="Miozoa" rank="species" species="grayi">
<emphasis italics="true" pageId="4" pageNumber="96">E. grayi</emphasis>
</taxonomicName>
are considerably larger than other coccidians with surface projections (
<tableCitation box="[946,1024,1229,1251]" captionStart="Table I" captionStartId="6.[117,167,202,221]" captionTargetPageId="6" captionText="Table I. Size (length and width in micrometers) of oocysts of Eimeria spp. with surface projections." httpUri="http://table.plazi.org/id/DF2F6623FFECFFC59415FF597C4B5F9F" pageId="4" pageNumber="96" tableUuid="DF2F6623FFECFFC59415FF597C4B5F9F">Table I</tableCitation>
).
</paragraph>
</subSubSection>
</treatment>
</document>