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<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.94.96301" ID-GBIF-Dataset="da44eeef-213c-4a7c-912e-0fe3ae3c0fd5" ID-Pensoft-Pub="1314-4049-94-17" ID-Pensoft-UUID="7473D183D2E756ABAF47666233DED6B8" ModsDocID="1314-4049-94-17" checkinTime="1669899185336" checkinUser="pensoft" docAuthor="Pham, Nam Q., Marincowitz, Seonju, Chen, ShuaiFei, Rodas, Carlos A. &amp; Wingfield, Michael J." docDate="2022" docId="3DCC8EAD8B6A50B8B7E68E2ADACBF40A" docLanguage="en" docName="MycoKeys 94: 17-35" docOrigin="MycoKeys 94" docPubDate="2022-11-30" docSource="http://dx.doi.org/10.3897/mycokeys.94.96301" docTitle="Calonectria exiguispora N. Q. Pham, Marinc. &amp; M. J. Wingf. 2022, sp. nov." docType="treatment" docVersion="2" id="7473D183D2E756ABAF47666233DED6B8" lastPageNumber="17" masterDocId="7473D183D2E756ABAF47666233DED6B8" masterDocTitle="Soil-borne Calonectria (Hypocreales, Nectriaceae) associated with Eucalyptus plantations in Colombia" masterLastPageNumber="35" masterPageNumber="17" pageNumber="17" updateTime="1669899790777" updateUser="ExternalLinkService">
<mods:mods xmlns:mods="http://www.loc.gov/mods/v3">
<mods:titleInfo>
<mods:title>Soil-borne Calonectria (Hypocreales, Nectriaceae) associated with Eucalyptus plantations in Colombia</mods:title>
</mods:titleInfo>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Pham, Nam Q.</mods:namePart>
<mods:nameIdentifier type="ORCID">https://orcid.org/0000-0002-4938-9067</mods:nameIdentifier>
<mods:affiliation>Department of Plant and Soil Sciences, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria, South Africa</mods:affiliation>
<mods:nameIdentifier type="email">nam.pham@fabi.up.ac.za</mods:nameIdentifier>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Marincowitz, Seonju</mods:namePart>
<mods:nameIdentifier type="ORCID">https://orcid.org/0000-0002-4726-1211</mods:nameIdentifier>
<mods:affiliation>Department of Plant and Soil Sciences, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria, South Africa</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Chen, ShuaiFei</mods:namePart>
<mods:nameIdentifier type="ORCID">https://orcid.org/0000-0002-3920-9982</mods:nameIdentifier>
<mods:affiliation>Department of Plant and Soil Sciences, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria, South Africa &amp; Department of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria, South Africa &amp; Research Institute of Fast-growing Trees (RIFT), Chinese Academy of Forestry (CAF), Zhanjiang, Guangdong Province, China</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Rodas, Carlos A.</mods:namePart>
<mods:nameIdentifier type="ORCID">https://orcid.org/0000-0002-8895-8883</mods:nameIdentifier>
<mods:affiliation>Department of Plant and Soil Sciences, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria, South Africa &amp; China Eucalypt Research Centre (CERC), Chinese Academy of Forestry (CAF), Zhanjiang, Guangdong Province, China</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Wingfield, Michael J.</mods:namePart>
<mods:nameIdentifier type="ORCID">https://orcid.org/0000-0001-9346-2009</mods:nameIdentifier>
<mods:affiliation>Department of Plant and Soil Sciences, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria, South Africa</mods:affiliation>
</mods:name>
<mods:typeOfResource>text</mods:typeOfResource>
<mods:relatedItem type="host">
<mods:titleInfo>
<mods:title>MycoKeys</mods:title>
</mods:titleInfo>
<mods:part>
<mods:date>2022</mods:date>
<mods:detail type="pubDate">
<mods:number>2022-11-30</mods:number>
</mods:detail>
<mods:detail type="volume">
<mods:number>94</mods:number>
</mods:detail>
<mods:extent unit="page">
<mods:start>17</mods:start>
<mods:end>35</mods:end>
</mods:extent>
</mods:part>
</mods:relatedItem>
<mods:location>
<mods:url>http://dx.doi.org/10.3897/mycokeys.94.96301</mods:url>
</mods:location>
<mods:classification>journal article</mods:classification>
<mods:identifier type="DOI">http://dx.doi.org/10.3897/mycokeys.94.96301</mods:identifier>
<mods:identifier type="Pensoft-Pub">1314-4049-94-17</mods:identifier>
<mods:identifier type="Pensoft-UUID">7473D183D2E756ABAF47666233DED6B8</mods:identifier>
</mods:mods>
<treatment LSID="urn:lsid:plazi:treatment:3DCC8EAD8B6A50B8B7E68E2ADACBF40A" httpUri="http://treatment.plazi.org/id/3DCC8EAD8B6A50B8B7E68E2ADACBF40A" lastPageNumber="17" pageId="0" pageNumber="17">
<subSubSection pageId="0" pageNumber="17" type="nomenclature">
<paragraph pageId="0" pageNumber="17">
<taxonomicName LSID="3DCC8EAD-8B6A-50B8-B7E6-8E2ADACBF40A" authority="N. Q. Pham, Marinc. &amp; M. J. Wingf." authorityName="N. Q. Pham, Marinc. &amp; M. J. Wingf." authorityYear="2022" class="Sordariomycetes" family="Nectriaceae" genus="Calonectria" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Calonectria exiguispora" order="Hypocreales" pageId="0" pageNumber="17" phylum="Ascomycota" rank="species" species="exiguispora" status="sp. nov.">Calonectria exiguispora N.Q. Pham, Marinc. &amp; M.J. Wingf.</taxonomicName>
<taxonomicNameLabel pageId="0" pageNumber="17">sp. nov.</taxonomicNameLabel>
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="17" type="description">
<paragraph pageId="0" pageNumber="17">
<figureCitation captionStart="Figure 4" captionStartId="F4" captionText="Figure 4. Micrographs of Calonectria exiguispora sp. nov. (ex-holotype: CMW 49752 = CMW-IA 160). A conidiophores formed on SNA B stipes and vesicles C conidiogenous apparatus D chlamydospores E conidia (x 400) F conidia (x 1 000). Scale bars: 100 µm (A); 50 µm (D, E); 25 µm (C); 10 µm (B, F)." figureDoi="10.3897/mycokeys.94.96301.figure4" httpUri="https://binary.pensoft.net/fig/776033" pageId="0" pageNumber="17">Figs 4</figureCitation>
<figureCitation captionStart="Figure 6" captionStartId="F6" captionText="Figure 6. Culture morphology of Calonectria exiguispora (A-D) at 25 ° C and C. guahibo (E-H) at 30 ° C in the dark for 6 d (A, C, E, G) and 30 d (B, E, F, H) at its optimum temperature A, B CMW 49752 (ex-holotype) C, D CMW 49753 E, F CMW 49791 (ex-holotype) G, H CMW 49782." figureDoi="10.3897/mycokeys.94.96301.figure6" httpUri="https://binary.pensoft.net/fig/776035" pageId="0" pageNumber="17">, 6A, B</figureCitation>
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="17" type="etymology">
<paragraph pageId="0" pageNumber="17">Etymology.</paragraph>
<paragraph pageId="0" pageNumber="17">
<normalizedToken originalValue="“exiguus”">&quot;exiguus&quot;</normalizedToken>
(Latin) = small +
<normalizedToken originalValue="“spora”">&quot;spora&quot;</normalizedToken>
(Latin) = spores, referring to the small macroconidia produced by this species.
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="17" type="diagnosis">
<paragraph pageId="0" pageNumber="17">Diagnosis.</paragraph>
<paragraph pageId="0" pageNumber="17">
Phylogenetically close to
<taxonomicName lsidName="C. piauiensis" pageId="0" pageNumber="17" rank="species" species="piauiensis">
<emphasis italics="true" pageId="0" pageNumber="17">C. piauiensis</emphasis>
</taxonomicName>
and
<taxonomicName lsidName="C. brassianae" pageId="0" pageNumber="17" rank="species" species="brassianae">
<emphasis italics="true" pageId="0" pageNumber="17">C. brassianae</emphasis>
</taxonomicName>
but differs in having smaller macroconidia.
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="17" type="materials_examined">
<paragraph pageId="0" pageNumber="17">
<typeStatus>Type</typeStatus>
.
</paragraph>
<paragraph pageId="0" pageNumber="17">
<materialsCitation accessionNumber="CMW 49752" collectingDate="2016-08" collectorName="Quinchia. Soils, C. A. Rodas." country="Colombia" location="GenBank" specimenCount="1" stateProvince="Risaralda" typeStatus="Holotype">
<collectingCountry name="Colombia">Colombia</collectingCountry>
:
<collectingRegion country="Colombia" name="Risaralda">Risaralda</collectingRegion>
,
<collectorName>
<normalizedToken originalValue="Quinchía">Quinchia</normalizedToken>
. Soils
</collectorName>
in
<taxonomicName class="Dothideomycetes" family="Botryosphaeriaceae" genus="Eucalyptus" kingdom="Fungi" lsidName="Eucalyptus" order="Botryosphaeriales" pageId="0" pageNumber="17" phylum="Ascomycota" rank="genus">
<emphasis italics="true" pageId="0" pageNumber="17">Eucalyptus</emphasis>
</taxonomicName>
plantation.
<collectingDate value="2016-08">August 2016</collectingDate>
.
<collectorName>C.A. Rodas.</collectorName>
(
<emphasis bold="true" italics="true" pageId="0" pageNumber="17">
<typeStatus>Holotype</typeStatus>
</emphasis>
PRU(M) 4501, stored in a metabolically inactive state;
<emphasis bold="true" pageId="0" pageNumber="17">
<emphasis italics="true" pageId="0" pageNumber="17">ex</emphasis>
-
<emphasis italics="true" pageId="0" pageNumber="17">
<typeStatus>holotype</typeStatus>
</emphasis>
</emphasis>
<accessionNumber httpUri="http://www.ncbi.nlm.nih.gov/protein/CMW49752">CMW 49752</accessionNumber>
, CMW-IA 160).
<location LSID="urn:lsid:plazi:treatment:3DCC8EAD8B6A50B8B7E68E2ADACBF40A:5252CDE7B196DFE9C91C32F733A8080F" country="Colombia" name="GenBank" stateProvince="Risaralda">GenBank</location>
</materialsCitation>
:
<materialsCitation accessionNumber="OP796405" collectingDate="2022-01-01" collectingDateMax="2022-12-31" collectingDateMin="2022-01-01" country="Colombia" specimenCount="1" stateProvince="Risaralda" typeStatus="holotype">
<accessionNumber httpUri="http://www.ncbi.nlm.nih.gov/nucleotide/OP796405">OP796405</accessionNumber>
(
<emphasis italics="true" pageId="0" pageNumber="17">ACT</emphasis>
)
</materialsCitation>
;
<materialsCitation accessionNumber="OP822275" collectingDate="2022-01-01" collectingDateMax="2022-12-31" collectingDateMin="2022-01-01" country="Colombia" specimenCount="1" stateProvince="Risaralda" typeStatus="holotype">
<accessionNumber httpUri="http://www.ncbi.nlm.nih.gov/nucleotide/OP822275">OP822275</accessionNumber>
(
<emphasis italics="true" pageId="0" pageNumber="17">CMDA</emphasis>
)
</materialsCitation>
;
<materialsCitation accessionNumber="OP822382" collectingDate="2022-01-01" collectingDateMax="2022-12-31" collectingDateMin="2022-01-01" country="Colombia" specimenCount="1" stateProvince="Risaralda" typeStatus="holotype">
<accessionNumber httpUri="http://www.ncbi.nlm.nih.gov/nucleotide/OP822382">OP822382</accessionNumber>
(
<emphasis italics="true" pageId="0" pageNumber="17">HIS3</emphasis>
)
</materialsCitation>
;
<materialsCitation accessionNumber="OP822489" collectingDate="2022-01-01" collectingDateMax="2022-12-31" collectingDateMin="2022-01-01" country="Colombia" specimenCount="1" stateProvince="Risaralda" typeStatus="holotype">
<accessionNumber httpUri="http://www.ncbi.nlm.nih.gov/nucleotide/OP822489">OP822489</accessionNumber>
(
<emphasis italics="true" pageId="0" pageNumber="17">RPB2</emphasis>
)
</materialsCitation>
;
<materialsCitation accessionNumber="OP822168" collectingDate="2022-01-01" collectingDateMax="2022-12-31" collectingDateMin="2022-01-01" country="Colombia" specimenCount="1" stateProvince="Risaralda" typeStatus="holotype">
<accessionNumber httpUri="http://www.ncbi.nlm.nih.gov/nucleotide/OP822168">OP822168</accessionNumber>
(
<emphasis italics="true" pageId="0" pageNumber="17">TEF1</emphasis>
)
</materialsCitation>
;
<materialsCitation accessionNumber="OP822596" collectingDate="2022-01-01" collectingDateMax="2022-12-31" collectingDateMin="2022-01-01" country="Colombia" specimenCount="1" stateProvince="Risaralda" typeStatus="holotype">
<accessionNumber httpUri="http://www.ncbi.nlm.nih.gov/nucleotide/OP822596">OP822596</accessionNumber>
(
<emphasis italics="true" pageId="0" pageNumber="17">TUB2</emphasis>
)
</materialsCitation>
.
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="17" type="description">
<paragraph pageId="0" pageNumber="17">Description.</paragraph>
<paragraph pageId="0" pageNumber="17">
<emphasis bold="true" italics="true" pageId="0" pageNumber="17">Sexual morph</emphasis>
not observed.
<emphasis bold="true" italics="true" pageId="0" pageNumber="17">Conidiophores</emphasis>
scarce on SNA, consisting of conidiogenous apparatus and stipes, branched or simple.
<emphasis bold="true" italics="true" pageId="0" pageNumber="17">Stipes</emphasis>
infrequent, elongated, septate, 75-273
<normalizedToken originalValue="µm">µm</normalizedToken>
long, 2-5
<normalizedToken originalValue="µm">µm</normalizedToken>
wide near base, tapering towards apex, simple or occasionally dichotomously branched, mostly being part of conidiogenous apparatus;
<emphasis bold="true" italics="true" pageId="0" pageNumber="17">vesicles</emphasis>
terminal, slightly inflate to ellipsoidal, 2-5
<normalizedToken originalValue="µm">µm</normalizedToken>
wide.
<emphasis bold="true" italics="true" pageId="0" pageNumber="17">Conidiogenous apparatus</emphasis>
hyaline, simple or branched in 1-3 (-4) tiers, uncommonly developing from stipes;
<emphasis bold="true" italics="true" pageId="0" pageNumber="17">main axis</emphasis>
upright, septate, 20-275
<normalizedToken originalValue="×">x</normalizedToken>
3-7
<normalizedToken originalValue="µm">µm</normalizedToken>
;
<emphasis bold="true" italics="true" pageId="0" pageNumber="17">branches</emphasis>
doliiform to cylindrical, primary branches 8-39
<normalizedToken originalValue="×">x</normalizedToken>
3-7
<normalizedToken originalValue="µm">µm</normalizedToken>
, secondary branches 8-24
<normalizedToken originalValue="×">x</normalizedToken>
2-6
<normalizedToken originalValue="µm">µm</normalizedToken>
, tertiary branches 10-23
<normalizedToken originalValue="×">x</normalizedToken>
2-5
<normalizedToken originalValue="µm">µm</normalizedToken>
, quarternary branches 10-14
<normalizedToken originalValue="×">x</normalizedToken>
3-4
<normalizedToken originalValue="µm">µm</normalizedToken>
.
<emphasis bold="true" italics="true" pageId="0" pageNumber="17">Conidiogenous cells</emphasis>
holoblastic, hyaline, discrete, cylindrical to tapered above, often constricted near base, with periclinal thickening, 8-20
<normalizedToken originalValue="×">x</normalizedToken>
3-5 (11.8
<normalizedToken originalValue="±">+/-</normalizedToken>
2.71
<normalizedToken originalValue="×">x</normalizedToken>
3.2
<normalizedToken originalValue="±">+/-</normalizedToken>
0.5)
<normalizedToken originalValue="µm">µm</normalizedToken>
.
<emphasis bold="true" italics="true" pageId="0" pageNumber="17">Macroconidia</emphasis>
hyaline, cylindrical, round at apex, 1-septate, septum sub-median or median, guttulate, 21-40
<normalizedToken originalValue="×">x</normalizedToken>
3-4 (30.9
<normalizedToken originalValue="±">+/-</normalizedToken>
4.09
<normalizedToken originalValue="×">x</normalizedToken>
3.5
<normalizedToken originalValue="±">+/-</normalizedToken>
0.23)
<normalizedToken originalValue="µm">µm</normalizedToken>
.
<emphasis bold="true" italics="true" pageId="0" pageNumber="17">Chlamydospores</emphasis>
present, scarce, in clumps or in chains.
<emphasis bold="true" italics="true" pageId="0" pageNumber="17">Mega</emphasis>
- and
<emphasis bold="true" italics="true" pageId="0" pageNumber="17">microconidia</emphasis>
not observed.
</paragraph>
<paragraph pageId="0" pageNumber="17">Colonies on 2% MEA in the dark for 6 d, white on surface, pale luteous in reverse, with moderate amount of aerial mycelium, with entire edges. Optimal growth temperature at 25 °C reaching 65.2 mm in 6 d, followed by 20 °C (57.3 mm), 15 °C (39.8 mm), 10 °C (19.7 mm), 5 °C (8.2 mm), no growth at 30 °C and 35 °C. Colonies kept at 30 °C and 35 °C being relocated to 25 °C for another 6 d revived (30 °C) and showed no growth (35 °C). Colonies on 2% MEA in the dark for 30 d, white to umber on surface, umber to dark brick in reverse, with flat mycelia.</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="17" type="distribution">
<paragraph pageId="0" pageNumber="17">Distribution.</paragraph>
<paragraph pageId="0" pageNumber="17">Colombia.</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="17" type="materials_examined">
<paragraph pageId="0" pageNumber="17">Material examined.</paragraph>
<paragraph pageId="0" pageNumber="17">
<materialsCitation accessionNumber="CMW 49753" collectingDate="2016-08" collectorName="C. A. Rodas." country="Colombia" location="Risaralda" specimenCount="1" stateProvince="Risaralda">
<collectingCountry name="Colombia">Colombia</collectingCountry>
:
<collectingRegion country="Colombia" name="Risaralda">Risaralda</collectingRegion>
,
<normalizedToken originalValue="Quinchía">Quinchia</normalizedToken>
. Soils in
<taxonomicName class="Dothideomycetes" family="Botryosphaeriaceae" genus="Eucalyptus" kingdom="Fungi" lsidName="Eucalyptus" order="Botryosphaeriales" pageId="0" pageNumber="17" phylum="Ascomycota" rank="genus">
<emphasis italics="true" pageId="0" pageNumber="17">Eucalyptus</emphasis>
</taxonomicName>
sp. plantation.
<collectingDate value="2016-08">August 2016</collectingDate>
.
<collectorName>C.A. Rodas.</collectorName>
(PRU(M) 4502, stored in a metabolically inactive state; culture
<accessionNumber httpUri="http://www.ncbi.nlm.nih.gov/protein/CMW49753">CMW 49753</accessionNumber>
, CMW-IA 161)
</materialsCitation>
.
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="17" type="notes">
<paragraph pageId="0" pageNumber="17">Notes.</paragraph>
<paragraph pageId="0" pageNumber="17">
<taxonomicName class="Sordariomycetes" family="Nectriaceae" genus="Calonectria" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Calonectria exiguispora" order="Hypocreales" pageId="0" pageNumber="17" phylum="Ascomycota" rank="species" species="exiguispora">
<emphasis italics="true" pageId="0" pageNumber="17">Calonectria exiguispora</emphasis>
</taxonomicName>
is a member of the
<taxonomicName lsidName="C. candelabrum" pageId="0" pageNumber="17" rank="species" species="candelabrum">
<emphasis italics="true" pageId="0" pageNumber="17">C. candelabrum</emphasis>
</taxonomicName>
species complex (
<bibRefCitation DOI="https://doi.org/10.1016/j.simyco.2020.08.001" author="Liu, QL" journalOrPublisher="Molecular Biology and Evolution" pageId="0" pageNumber="17" refId="B19" refString="Liu, QL, Li, JQ, Wingfield, MJ, Duong, TA, Wingfield, BD, Crous, PW, Chen, SF, 2020. Reconsideration of species boundaries and proposed DNA barcodes for Calonectria. Studies in Mycology 97: 100106. https://doi.org/10.1016/j.simyco.2020.08.001" title="Reconsideration of species boundaries and proposed DNA barcodes for Calonectria. Studies in Mycology 97: 100106." url="https://doi.org/10.1016/j.simyco.2020.08.001" year="2020">Liu et al. 2020</bibRefCitation>
). It shares some characteristics with other species in the complex, such as 1-septate macroconidia and 'ellipsoidal to
<normalizedToken originalValue="obpyriform">obpyriform'</normalizedToken>
shape vesicle. However, it can be distinguished from most species in the complex by its smaller conidial dimensions (21-40
<normalizedToken originalValue="×">x</normalizedToken>
3-4
<normalizedToken originalValue="µm">µm</normalizedToken>
, avg. 30.9
<normalizedToken originalValue="×">x</normalizedToken>
3.5
<normalizedToken originalValue="µm">µm</normalizedToken>
) except for
<taxonomicName lsidName="C. brevistipitata" pageId="0" pageNumber="17" rank="species" species="brevistipitata">
<emphasis italics="true" pageId="0" pageNumber="17">C. brevistipitata</emphasis>
</taxonomicName>
(29-35
<normalizedToken originalValue="×">x</normalizedToken>
3-4
<normalizedToken originalValue="µm">µm</normalizedToken>
, avg. 31
<normalizedToken originalValue="×">x</normalizedToken>
3.5
<normalizedToken originalValue="µm">µm</normalizedToken>
, isolated from Mexican soil) and
<taxonomicName lsidName="C. stipitate" pageId="0" pageNumber="17" rank="species" species="stipitate">
<emphasis italics="true" pageId="0" pageNumber="17">C. stipitate</emphasis>
</taxonomicName>
(27-37
<normalizedToken originalValue="×">x</normalizedToken>
3-6, avg. 32
<normalizedToken originalValue="×">x</normalizedToken>
4
<normalizedToken originalValue="µm">µm</normalizedToken>
, isolated from Colombian
<taxonomicName class="Dothideomycetes" family="Botryosphaeriaceae" genus="Eucalyptus" kingdom="Fungi" lsidName="Eucalyptus" order="Botryosphaeriales" pageId="0" pageNumber="17" phylum="Ascomycota" rank="genus">
<emphasis italics="true" pageId="0" pageNumber="17">Eucalyptus</emphasis>
</taxonomicName>
sp.) (
<bibRefCitation DOI="https://doi.org/10.1016/j.simyco.2016.11.004" author="Lombard, L" journalOrPublisher="Studies in Mycology" pageId="0" pageNumber="17" pagination="159 - 198" refId="B24" refString="Lombard, L, Wingfield, MJ, Alfenas, AC, Crous, PW, 2016. The forgotten Calonectria collection: Pouring old wine into new bags. Studies in Mycology 85 (1): 159 - 198, DOI: https://doi.org/10.1016/j.simyco.2016.11.004" title="The forgotten Calonectria collection: Pouring old wine into new bags." url="https://doi.org/10.1016/j.simyco.2016.11.004" volume="85" year="2016">Lombard et al. 2016</bibRefCitation>
). Nevertheless, these two species are distantly related to
<taxonomicName lsidName="C. exiguispora" pageId="0" pageNumber="17" rank="species" species="exiguispora">
<emphasis italics="true" pageId="0" pageNumber="17">C. exiguispora</emphasis>
</taxonomicName>
(Fig.
<figureCitation captionStart="Figure 3" captionStartId="F3" captionText="Figure 3. Phylogenetic tree based on maximum likelihood (ML) analysis of a combined DNA data set of ACT, CMDA, HIS 3, RPB 2, TEF 1 and TUB 2 sequences for Calonectria spp. Bootstrap values ≥ 70 % for ML analyses and posterior probabilities values ≥ 0.90 obtained from Bayesian inference (BI) are indicated at the nodes as ML / BI. Bootstrap values &lt;70 % or probabilities values &lt;0.90 are marked with &quot; * &quot;, and nodes lacking the support values are marked with &quot; - &quot;. Isolates representing ex-type material are marked with &quot; T &quot;. Curvicladiella cignea (isolate CBS 109167 and CBS 109168) represents the outgroup." figureDoi="10.3897/mycokeys.94.96301.figure3" httpUri="https://binary.pensoft.net/fig/776032" pageId="0" pageNumber="17">3</figureCitation>
). Recently
<bibRefCitation DOI="https://doi.org/10.1016/j.simyco.2020.08.001" author="Liu, QL" journalOrPublisher="Molecular Biology and Evolution" pageId="0" pageNumber="17" refId="B19" refString="Liu, QL, Li, JQ, Wingfield, MJ, Duong, TA, Wingfield, BD, Crous, PW, Chen, SF, 2020. Reconsideration of species boundaries and proposed DNA barcodes for Calonectria. Studies in Mycology 97: 100106. https://doi.org/10.1016/j.simyco.2020.08.001" title="Reconsideration of species boundaries and proposed DNA barcodes for Calonectria. Studies in Mycology 97: 100106." url="https://doi.org/10.1016/j.simyco.2020.08.001" year="2020">Liu et al. (2020)</bibRefCitation>
reduced
<taxonomicName lsidName="C. stipitata" pageId="0" pageNumber="17" rank="species" species="stipitata">
<emphasis italics="true" pageId="0" pageNumber="17">C. stipitata</emphasis>
</taxonomicName>
to synonymy with
<taxonomicName lsidName="C. spathulata" pageId="0" pageNumber="17" rank="species" species="spathulata">
<emphasis italics="true" pageId="0" pageNumber="17">C. spathulata</emphasis>
</taxonomicName>
, the conidial dimensions of which range from 48-100
<normalizedToken originalValue="×">x</normalizedToken>
4-6
<normalizedToken originalValue="µm">µm</normalizedToken>
(avg. 80
<normalizedToken originalValue="×">x</normalizedToken>
6
<normalizedToken originalValue="µm">µm</normalizedToken>
). They regarded the smaller conidial dimensions of
<emphasis italics="true" pageId="0" pageNumber="17">
<taxonomicName genus="C." lsidName="C." pageId="0" pageNumber="17" rank="genus">C.</taxonomicName>
&quot;
<taxonomicName class="Sordariomycetes" family="Nectriaceae" genus="Calonectria" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Calonectria stipitata" order="Hypocreales" pageId="0" pageNumber="17" phylum="Ascomycota" rank="species" species="stipitata">Calonectria stipitata</taxonomicName>
</emphasis>
&quot; as representing intraspecific variation.
<taxonomicName class="Sordariomycetes" family="Nectriaceae" genus="Calonectria" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Calonectria exiguispora" order="Hypocreales" pageId="0" pageNumber="17" phylum="Ascomycota" rank="species" species="exiguispora">
<emphasis italics="true" pageId="0" pageNumber="17">Calonectria exiguispora</emphasis>
</taxonomicName>
is phylogenetically closely related to
<taxonomicName lsidName="C. piauiensis" pageId="0" pageNumber="17" rank="species" species="piauiensis">
<emphasis italics="true" pageId="0" pageNumber="17">C. piauiensis</emphasis>
</taxonomicName>
and
<taxonomicName lsidName="C. brassianae" pageId="0" pageNumber="17" rank="species" species="brassianae">
<emphasis italics="true" pageId="0" pageNumber="17">C. brassianae</emphasis>
</taxonomicName>
, which were isolated from soils associated with
<taxonomicName class="Dothideomycetes" family="Botryosphaeriaceae" genus="Eucalyptus" kingdom="Fungi" lsidName="Eucalyptus brassiana" order="Botryosphaeriales" pageId="0" pageNumber="17" phylum="Ascomycota" rank="species" species="brassiana">
<emphasis italics="true" pageId="0" pageNumber="17">Eucalyptus brassiana</emphasis>
</taxonomicName>
trees in Brazil (
<bibRefCitation DOI="https://doi.org/10.1016/j.simyco.2014.11.002" author="Alfenas, RF" journalOrPublisher="Studies in Mycology" pageId="0" pageNumber="17" pagination="89 - 130" refId="B2" refString="Alfenas, RF, Lombard, L, Pereira, OL, Alfenas, AC, Crous, PW, 2015. Diversity and potential impact of Calonectria species in Eucalyptus plantations in Brazil. Studies in Mycology 80 (1): 89 - 130, DOI: https://doi.org/10.1016/j.simyco.2014.11.002" title="Diversity and potential impact of Calonectria species in Eucalyptus plantations in Brazil." url="https://doi.org/10.1016/j.simyco.2014.11.002" volume="80" year="2015">Alfenas et al. 2015</bibRefCitation>
). These two species, however, have much larger conidial dimensions:
<taxonomicName lsidName="C. piauiensis" pageId="0" pageNumber="17" rank="species" species="piauiensis">
<emphasis italics="true" pageId="0" pageNumber="17">C. piauiensis</emphasis>
</taxonomicName>
(38-60
<normalizedToken originalValue="×">x</normalizedToken>
3-5
<normalizedToken originalValue="µm">µm</normalizedToken>
, avg. 49
<normalizedToken originalValue="×">x</normalizedToken>
4.5
<normalizedToken originalValue="µm">µm</normalizedToken>
) and
<taxonomicName lsidName="C. brassianae" pageId="0" pageNumber="17" rank="species" species="brassianae">
<emphasis italics="true" pageId="0" pageNumber="17">C. brassianae</emphasis>
</taxonomicName>
(35-65
<normalizedToken originalValue="×">x</normalizedToken>
3-5
<normalizedToken originalValue="µm">µm</normalizedToken>
, avg. 53
<normalizedToken originalValue="×">x</normalizedToken>
4
<normalizedToken originalValue="µm">µm</normalizedToken>
) (
<bibRefCitation DOI="https://doi.org/10.1016/j.simyco.2014.11.002" author="Alfenas, RF" journalOrPublisher="Studies in Mycology" pageId="0" pageNumber="17" pagination="89 - 130" refId="B2" refString="Alfenas, RF, Lombard, L, Pereira, OL, Alfenas, AC, Crous, PW, 2015. Diversity and potential impact of Calonectria species in Eucalyptus plantations in Brazil. Studies in Mycology 80 (1): 89 - 130, DOI: https://doi.org/10.1016/j.simyco.2014.11.002" title="Diversity and potential impact of Calonectria species in Eucalyptus plantations in Brazil." url="https://doi.org/10.1016/j.simyco.2014.11.002" volume="80" year="2015">Alfenas et al. 2015</bibRefCitation>
). It can be differentiated from its most closely related species by sequences of
<emphasis italics="true" pageId="0" pageNumber="17">ACT</emphasis>
,
<emphasis italics="true" pageId="0" pageNumber="17">CMDA</emphasis>
,
<emphasis italics="true" pageId="0" pageNumber="17">HIS3</emphasis>
,
<emphasis italics="true" pageId="0" pageNumber="17">RPB2</emphasis>
,
<emphasis italics="true" pageId="0" pageNumber="17">TEF1</emphasis>
and
<emphasis italics="true" pageId="0" pageNumber="17">TUB2</emphasis>
gene regions.
</paragraph>
<caption doi="10.3897/mycokeys.94.96301.figure4" httpUri="https://binary.pensoft.net/fig/776033" pageId="0" pageNumber="17" start="Figure 4" startId="F4">
<paragraph pageId="0" pageNumber="17">
<emphasis bold="true" pageId="0" pageNumber="17">Figure 4.</emphasis>
Micrographs of
<taxonomicName class="Sordariomycetes" family="Nectriaceae" genus="Calonectria" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Calonectria exiguispora" order="Hypocreales" pageId="0" pageNumber="17" phylum="Ascomycota" rank="species" species="exiguispora">
<emphasis italics="true" pageId="0" pageNumber="17">Calonectria exiguispora</emphasis>
</taxonomicName>
sp. nov. (ex-holotype: CMW 49752 = CMW-IA 160).
<emphasis bold="true" pageId="0" pageNumber="17">A</emphasis>
conidiophores formed on SNA
<emphasis bold="true" pageId="0" pageNumber="17">B</emphasis>
stipes and vesicles
<emphasis bold="true" pageId="0" pageNumber="17">C</emphasis>
conidiogenous apparatus
<emphasis bold="true" pageId="0" pageNumber="17">D</emphasis>
chlamydospores
<emphasis bold="true" pageId="0" pageNumber="17">E</emphasis>
conidia (
<normalizedToken originalValue="×">x</normalizedToken>
400)
<emphasis bold="true" pageId="0" pageNumber="17">F</emphasis>
conidia (
<normalizedToken originalValue="×">x</normalizedToken>
1 000). Scale bars: 100
<normalizedToken originalValue="µm">µm</normalizedToken>
(
<emphasis bold="true" pageId="0" pageNumber="17">A</emphasis>
); 50
<normalizedToken originalValue="µm">µm</normalizedToken>
(
<emphasis bold="true" pageId="0" pageNumber="17">D, E</emphasis>
); 25
<normalizedToken originalValue="µm">µm</normalizedToken>
(
<emphasis bold="true" pageId="0" pageNumber="17">C</emphasis>
); 10
<normalizedToken originalValue="µm">µm</normalizedToken>
(
<emphasis bold="true" pageId="0" pageNumber="17">B, F</emphasis>
).
</paragraph>
</caption>
</subSubSection>
</treatment>
</document>