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<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.32.23831" ID-GBIF-Dataset="49c4aa61-7d5b-4af2-9a4e-90c3347a4bd1" ID-PMC="PMC5904524" ID-Pensoft-Pub="1314-4049-32-65" ID-PubMed="29681738" ModsDocAuthor="" ModsDocDate="2018" ModsDocID="1314-4049-32-65" ModsDocOrigin="MycoKeys 32" ModsDocTitle="A multi-gene phylogeny of Chlorophyllum (Agaricaceae, Basidiomycota): new species, new combination and infrageneric classification" checkinTime="1521626549072" checkinUser="pensoft" docAuthor="Ge, Zai-Wei, Jacobs, Adriaana, Vellinga, Else C., Sysouphanthong, Phongeun, Walt, Retha van der, Lavorato, Carmine, An, Yi-Feng &amp; Yang, Zhu L." docDate="2018" docId="056D50F00772A5575F11DC1FF15E0BA6" docLanguage="en" docName="MycoKeys 32: 65-90" docOrigin="MycoKeys 32" docSource="http://dx.doi.org/10.3897/mycokeys.32.23831" docTitle="Chlorophyllum globosum Vellinga" docType="treatment" docVersion="4" lastPageNumber="76" masterDocId="FFBBD77C4607FFB6FFF7FF8DFFFC017A" masterDocTitle="A multi-gene phylogeny of Chlorophyllum (Agaricaceae, Basidiomycota): new species, new combination and infrageneric classification" masterLastPageNumber="90" masterPageNumber="65" pageNumber="75" updateTime="1668135969713" updateUser="ExternalLinkService">
<mods:mods xmlns:mods="http://www.loc.gov/mods/v3">
<mods:titleInfo>
<mods:title>A multi-gene phylogeny of Chlorophyllum (Agaricaceae, Basidiomycota): new species, new combination and infrageneric classification</mods:title>
</mods:titleInfo>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Ge, Zai-Wei</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Jacobs, Adriaana</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Vellinga, Else C.</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Sysouphanthong, Phongeun</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Walt, Retha van der</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Lavorato, Carmine</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>An, Yi-Feng</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Yang, Zhu L.</mods:namePart>
</mods:name>
<mods:typeOfResource>text</mods:typeOfResource>
<mods:relatedItem type="host">
<mods:titleInfo>
<mods:title>MycoKeys</mods:title>
</mods:titleInfo>
<mods:part>
<mods:date>2018</mods:date>
<mods:detail type="volume">
<mods:number>32</mods:number>
</mods:detail>
<mods:extent unit="page">
<mods:start>65</mods:start>
<mods:end>90</mods:end>
</mods:extent>
</mods:part>
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<mods:location>
<mods:url>http://dx.doi.org/10.3897/mycokeys.32.23831</mods:url>
</mods:location>
<mods:classification>journal article</mods:classification>
<mods:identifier type="DOI">http://dx.doi.org/10.3897/mycokeys.32.23831</mods:identifier>
<mods:identifier type="Pensoft-Pub">1314-4049-32-65</mods:identifier>
</mods:mods>
<treatment ID-GBIF-Taxon="142462596" LSID="urn:lsid:plazi:treatment:056D50F00772A5575F11DC1FF15E0BA6" httpUri="http://treatment.plazi.org/id/056D50F00772A5575F11DC1FF15E0BA6" lastPageId="11" lastPageNumber="76" pageId="10" pageNumber="75">
<subSubSection pageId="10" pageNumber="75" type="nomenclature">
<paragraph pageId="10" pageNumber="75">
<taxonomicName authority="(Mossebo) Vellinga" class="Agaricomycetes" family="Agaricaceae" genus="Chlorophyllum" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Chlorophyllum globosum" order="Agaricales" pageId="10" pageNumber="75" phylum="Basidiomycota" rank="species" species="globosum">Chlorophyllum globosum (Mossebo) Vellinga</taxonomicName>
</paragraph>
</subSubSection>
<subSubSection pageId="10" pageNumber="75" type="materials_examined">
<paragraph pageId="10" pageNumber="75">Type.</paragraph>
<paragraph pageId="10" pageNumber="75">
CAMEROON.
<normalizedToken originalValue="Yaoundé">Yaounde</normalizedToken>
, alt. 780 m, growing on humus in shade under tree, 1 November, 1996, D.
<taxonomicName genus="C." lsidName="C." pageId="10" pageNumber="75" rank="genus">C.</taxonomicName>
Mossebo, D.
<taxonomicName genus="C." lsidName="C." pageId="10" pageNumber="75" rank="genus">C.</taxonomicName>
Mossebo 98-1 kept in the Herbarium of University of
<normalizedToken originalValue="Yaoundé">Yaounde</normalizedToken>
I (non vide).
</paragraph>
</subSubSection>
<subSubSection lastPageId="11" lastPageNumber="76" pageId="10" pageNumber="75" type="description">
<paragraph pageId="10" pageNumber="75">Description.</paragraph>
<paragraph pageId="10" pageNumber="75">
Basidiocarps medium to large-sized (Figure 3C). Pileus 5.0-20.0 cm broad, ovoid to subglobose when young, expanding to parabolic, convex to broadly convex with age; margin inflexed, with short, fine striations; surface covered with yellowish-white (3A2) to yellowish-grey (4A2), greyish-yellow (4B3-4B4), brownish-orange (6C6) to greyish-brown (5D3) squamules. The squamules remain intact at disc, but elsewhere diffract with expansion and recede from pileus margin, displaying the white to yellowish-white (2A2, 3A2, 4A2) felted or fibrillose background which turned pastel red to red (9A4-6) when touched. Lamellae free and remote from stipe with obvious gutter, white to orange-white (5A1-2) when young, turning pastel red to red (9A4-6) when touched, pastel green to greyish-green (29A4, 29B4) when fully mature, crowded, ventricose and narrow near pileal margin, crowded, up to 8 mm wide, with 1-2 series of lamellulae; edge finely fimbriate, white to yellowish-grey (4A2). Stipe 8.5-28.0
<normalizedToken originalValue="×">x</normalizedToken>
1.0-3.1 cm, subcylindric, tapering to apex, with bulb-like, 3.0-3.4 cm wide; glabrous, white to brownish-orange (6C3-6), hollow, nearly stuffed, with an annulus about 1/3 away from the stipe apex (Figure 3C); sometimes with distant white fibrillose at apex zone, turning pastel red to red (9A4-6) when touched, with white rhizomorph connected to substrate. Context thick, white in pileus and stipe, brownish-orange (6C3-6) at apex zone, paler to middle zone and white downward base, discolouring pastel red to red (9A4-6) in both pileus and stipe context when bruised, with mushroom odour. Taste mild. Spore print yellowish-white (2A2) to pale yellow (2A3) to greyish-green (29D3-5, 29D5-6).
</paragraph>
<paragraph pageId="11" pageNumber="76">
<pageBreakToken pageId="11" pageNumber="76" start="start">Basidiospores</pageBreakToken>
[40,2,2] (10.5)11.5-12.0 (12.5)
<normalizedToken originalValue="×">x</normalizedToken>
(8.0) 8.5-9.0
<normalizedToken originalValue="μm">μm</normalizedToken>
(mean 11.8
<normalizedToken originalValue="±">+/-</normalizedToken>
0.4
<normalizedToken originalValue="×">x</normalizedToken>
8.7
<normalizedToken originalValue="±">+/-</normalizedToken>
0.3
<normalizedToken originalValue="μm">μm</normalizedToken>
), Q = 1.3-1.4 (1.5), Qav = 1.4
<normalizedToken originalValue="±">+/-</normalizedToken>
0.05, broadly amygdaliform in side view, ovoid in frontal view, with truncate apex, smooth, greenish-white (28A2), congophilous, dextrinoid, thick-walled (Figure 6A), becoming purplish-red (14A6-14A7) in cresyl blue. Basidia 29-38
<normalizedToken originalValue="×">x</normalizedToken>
12.0-14.0
<normalizedToken originalValue="µm">µm</normalizedToken>
, clavate, hyaline, 4-spored, sometimes 2-spored, rarely 1-spored. Cheilocystidia 42-65
<normalizedToken originalValue="×">x</normalizedToken>
(15.0)18.0-29.0
<normalizedToken originalValue="µm">µm</normalizedToken>
, clavate occasionally with slightly long stalk, hyaline, sometimes with greyish-yellow vacuolar pigments. Pleurocystidia absent. Pileipellis a hymenidermal layer made up of subcylindrical hyphae (5.0-11.0
<normalizedToken originalValue="µm">µm</normalizedToken>
in diam.), slightly thick walled, with dull yellow (3B3) vacuolar pigments; terminal elements with rounded or attenuate apex, mostly narrowly clavate. Clamp connections not observed.
</paragraph>
<caption pageId="11" pageNumber="76">
<paragraph pageId="11" pageNumber="76">
Figure 6. Micro-morphological features of
<taxonomicName lsidName="C. globosum" pageId="11" pageNumber="76" rank="species" species="globosum">C. globosum</taxonomicName>
(HKAS 52741). A Basidiospores B Basidia C Cheilocystidia D Elements of squamules on pileus. Scale bars: 10
<normalizedToken originalValue="μm">μm</normalizedToken>
(A, D); 20
<normalizedToken originalValue="μm">μm</normalizedToken>
(B, C).
</paragraph>
</caption>
</subSubSection>
<subSubSection pageId="11" pageNumber="76" type="distribution">
<paragraph pageId="11" pageNumber="76">Distribution.</paragraph>
<paragraph pageId="11" pageNumber="76">Known from Cameroon, Nigeria and South Africa in Africa and from China, India and Thailand in Asia.</paragraph>
</subSubSection>
<subSubSection pageId="11" pageNumber="76" type="ecology">
<paragraph pageId="11" pageNumber="76">Ecology.</paragraph>
<paragraph pageId="11" pageNumber="76">Saprotrophic, solitary to scattered, terrestrial.</paragraph>
</subSubSection>
<subSubSection pageId="11" pageNumber="76" type="materials_examined">
<paragraph pageId="11" pageNumber="76">Specimens examined.</paragraph>
<paragraph pageId="11" pageNumber="76">
CHINA. Yunnan Province: between Yuanmou and Yongren, 28 June 2006, Z.W. Ge 2006-1 (HKAS 52741). SOUTH AFRICA. 2229 BD Kamkusi, Farm Ludwigslust 163 MS,
<geoCoordinate direction="south" orientation="latitude" precision="9" value="-22.271166">22°16.27'S</geoCoordinate>
,
<geoCoordinate direction="east" orientation="longitude" precision="9" value="29.817">29°49.02'E</geoCoordinate>
, alt. ca. 580 m, 12 March 2014, Van Der Walt, R 957 (PREM 62147), growing in sandy soil under Sickle bush (
<taxonomicName class="Magnoliopsida" family="Fabaceae" genus="Dichrostachys" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Dichrostachys cinerea" order="Fabales" pageId="11" pageNumber="76" phylum="Tracheophyta" rank="species" species="cinerea">Dichrostachys cinerea</taxonomicName>
) and Umbrella thorn trees (
<taxonomicName class="Magnoliopsida" family="Fabaceae" genus="Vachellia" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Vachellia tortilis" order="Fabales" pageId="11" pageNumber="76" phylum="Tracheophyta" rank="species" species="tortilis">Vachellia tortilis</taxonomicName>
, formerly
<taxonomicName class="Magnoliopsida" family="Fabaceae" genus="Acacia" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Acacia tortilis" order="Fabales" pageId="11" pageNumber="76" phylum="Tracheophyta" rank="species" species="tortilis">Acacia tortilis</taxonomicName>
); 2229 BD Kamkusi, Farm Ludwigslust 163 MS, alt. 584m, growing in sandy soil under Sickle bush and Umbrella thorn trees, 7 February 2014, Van Der Walt, R 869 (PREM 62148); same locality, 9 March 2014, Van Der Walt, R 936 (PREM 62149); 2229 BB Beit Bridge, Farm Matolege 133 MS, alt. ca. 580m, shady area under blue thorn (
<taxonomicName class="Magnoliopsida" family="Fabaceae" genus="Senegalia" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Senegalia erubescens" order="Fabales" pageId="11" pageNumber="76" phylum="Tracheophyta" rank="species" species="erubescens">Senegalia erubescens</taxonomicName>
, formerly
<taxonomicName class="Magnoliopsida" family="Fabaceae" genus="Acacia" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Acacia erubescens" order="Fabales" pageId="11" pageNumber="76" phylum="Tracheophyta" rank="species" species="erubescens">Acacia erubescens</taxonomicName>
), compost-rich, adjacent to lawn in hunting camp, 12 February 2014, Van Der Walt, R 892 (PREM 62150); 2229 BB Beit Bridge, Farm Wimpsh 139 MS, alt. ca. 604 m, loam soil, amongst grass - adjacent to seasonally waterlogged pan, 12 January 2014, Van Der Walt, R 821 (PREM 62151); same locality, 14 February 2014, Van Der Walt, R 900 (PREM 62152). THAILAND. Chiang Mai Province: Mae Taeng District, Pongduad Village,
<geoCoordinate direction="north" orientation="latitude" precision="925" value="16.1">16°06'N</geoCoordinate>
,
<geoCoordinate direction="east" orientation="longitude" precision="925" value="99.71667">99°43'E</geoCoordinate>
, 780-810 m, 16 June 2010, P. Sysouphanthong, P37 (MFLU100555); Chiang Rai Province: Muang District, Ratjabhat University campus, 30 August 2012, P. Sysouphanthong, 2012-21 (MFLU121815).
</paragraph>
</subSubSection>
<subSubSection pageId="11" pageNumber="76" type="discussion">
<paragraph pageId="11" pageNumber="76">Discussion.</paragraph>
<paragraph pageId="11" pageNumber="76">
<taxonomicName class="Agaricomycetes" family="Agaricaceae" genus="Chlorophyllum" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Chlorophyllum globosum" order="Agaricales" pageId="11" pageNumber="76" phylum="Basidiomycota" rank="species" species="globosum">Chlorophyllum globosum</taxonomicName>
was originally described from Cameroon in the genus
<taxonomicName class="Agaricomycetes" family="Agaricaceae" genus="Macrolepiota" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Macrolepiota" order="Agaricales" pageId="11" pageNumber="76" phylum="Basidiomycota" rank="genus">Macrolepiota</taxonomicName>
. It was said to differ from
<taxonomicName class="Agaricomycetes" family="Agaricaceae" genus="Macrolepiota" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Macrolepiota odorata" order="Agaricales" pageId="11" pageNumber="76" phylum="Basidiomycota" rank="species" species="odorata">Macrolepiota odorata</taxonomicName>
&quot;by the globose pileus and the ochraceous spore print&quot; (
<bibRefCitation author="Mossebo, DC" journalOrPublisher="Mycotaxon" pageId="16" pageNumber="81" pagination="267 - 278" title="Macrolepiotaglobosa et Nothopanusnsimalenensis, deux nouvelles especes fongiques du Cameroun." volume="76" year="2000">Mossebo et al. 2000</bibRefCitation>
). In fact, the pileus does not stay globose during maturation but becomes broadly convex (Figure 3C). Based on the morphological characters such as the truncate basidiospores and its phylogenetic position,
<bibRefCitation author="Vellinga, EC" journalOrPublisher="Mycotaxon" pageId="16" pageNumber="81" pagination="415 - 417" title="New combinations in Chlorophyllum." volume="83" year="2002">Vellinga (2002)</bibRefCitation>
transferred it to
<taxonomicName class="Agaricomycetes" family="Agaricaceae" genus="Chlorophyllum" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Chlorophyllum" order="Agaricales" pageId="11" pageNumber="76" phylum="Basidiomycota" rank="genus">Chlorophyllum</taxonomicName>
. The
<normalizedToken originalValue="authors">authors'</normalizedToken>
molecular phylogeny confirms that
<taxonomicName lsidName="C. globosum" pageId="11" pageNumber="76" rank="species" species="globosum">C. globosum</taxonomicName>
nests in
<taxonomicName class="Agaricomycetes" family="Agaricaceae" genus="Chlorophyllum" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Chlorophyllum" order="Agaricales" pageId="11" pageNumber="76" phylum="Basidiomycota" rank="genus">Chlorophyllum</taxonomicName>
close to
<taxonomicName lsidName="C. molybdites" pageId="11" pageNumber="76" rank="species" species="molybdites">C. molybdites</taxonomicName>
, but it differs from the latter in having a pale yellow spore print and clavate cheilocystidia. This species was first described from Africa, but its presence in several Asian countries is confirmed.
</paragraph>
</subSubSection>
</treatment>
</document>