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<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.60.38186" ID-GBIF-Dataset="ce86c4e9-3eff-41fa-a6db-47888a494228" ID-PMC="PMC6838221" ID-Pensoft-Pub="1314-4049-60-45" ID-Pensoft-UUID="8003A6139B1050888874E5F93ABFF322" ID-PubMed="31723337" ModsDocID="1314-4049-60-45" checkinTime="1572595447015" checkinUser="pensoft" docAuthor="Wang, Xin-Cun, Liu, Tie-Zhi, Chen, Shuang-Lin, Li, Yi &amp; Zhuang, Wen-Ying" docDate="2019" docId="B26640F5749E58279B98508F5CAF8DE7" docLanguage="en" docName="MycoKeys 60: 45-67" docOrigin="MycoKeys 60" docSource="http://dx.doi.org/10.3897/mycokeys.60.38186" docTitle="Helvella sichuanensis X. C. Wang &amp; W. Y. Zhuang, sp. nov." docType="treatment" docVersion="5" id="8003A6139B1050888874E5F93ABFF322" lastPageNumber="45" masterDocId="8003A6139B1050888874E5F93ABFF322" masterDocTitle="A four-locus phylogeny of rib-stiped cupulate species of Helvella (Helvellaceae, Pezizales) with discovery of three new species" masterLastPageNumber="67" masterPageNumber="45" pageNumber="45" updateTime="1732707536042" updateUser="ExternalLinkService">
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<mods:title id="2EA2D88EE8977874448DD2277FA34DB6">A four-locus phylogeny of rib-stiped cupulate species of Helvella (Helvellaceae, Pezizales) with discovery of three new species</mods:title>
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<mods:namePart id="BD8860F888856572FCAE6716BC6B79C8">Wang, Xin-Cun</mods:namePart>
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<mods:namePart id="7AB14366592F5DC971C692178C8A167D">Liu, Tie-Zhi</mods:namePart>
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<mods:namePart id="DC7192DBADEFAEF4CFEEEF1931B4CB54">Chen, Shuang-Lin</mods:namePart>
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<mods:namePart id="CF559BC4FA3FD1391F9EFC1B86C1F607">Li, Yi</mods:namePart>
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<mods:namePart id="8CD039C2D4B87B7F9580C18FF0C9E20D">Zhuang, Wen-Ying</mods:namePart>
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<treatment id="B26640F5749E58279B98508F5CAF8DE7" ID-GBIF-Taxon="160104920" LSID="urn:lsid:plazi:treatment:B26640F5749E58279B98508F5CAF8DE7" httpUri="http://treatment.plazi.org/id/B26640F5749E58279B98508F5CAF8DE7" lastPageNumber="45" pageId="0" pageNumber="45" scope_family="Helvellaceae" scope_order="Pezizales">
<subSubSection id="8F5BE1C5047C2E611C0EA76BF7266C23" pageId="0" pageNumber="45" type="nomenclature">
<paragraph id="2A75950D5E7036A9BB4444267D4C7018" pageId="0" pageNumber="45">
<taxonomicName id="F5D3E10D276934584D9D870BD8D7F6AA" LSID="B26640F5-749E-5827-9B98-508F5CAF8DE7" authority="X. C. Wang &amp; W. Y. Zhuang" class="Pezizomycetes" family="Helvellaceae" genus="Helvella" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Helvella sichuanensis" order="Pezizales" pageId="0" pageNumber="45" phylum="Ascomycota" rank="species" species="sichuanensis">Helvella sichuanensis X.C. Wang &amp; W.Y. Zhuang</taxonomicName>
<taxonomicNameLabel id="047AC299364BB6F6CB1137958750C42C" pageId="0" pageNumber="45">sp. nov.</taxonomicNameLabel>
<figureCitation id="6F9D4B553289137D32E253F77F05D4D2" captionStart="Figure 3" captionStartId="F3" captionText="Figure 3. a-d Helvella acetabuloides: a mature apothecia when dry (CFSZ 2044) b asci (HMAS 23842) c, d ascospores in ascus (c: CFSZ 2044, d: HMAS 23842) e-g Helvella sichuanensis (HMAS 254610): e mature apothecia when dry f mature apothecia when fresh g ascospores in asci h-k Helvella tianshanensis (HMAS 86040): h, i Mature apothecium when dry j asci k ascospores in ascus. Scale bars: 1 cm (a, e); 0.75 cm (h, i); 50 μm (b, g, j); 20 μm (c, d, k)." figureDoi="10.3897/mycokeys.60.38186.figure3" httpUri="https://binary.pensoft.net/fig/352439" pageId="0" pageNumber="45" tableDoi="10.3897/mycokeys.60.38186.figure3">
Figure 3
<normalizedToken id="B388F73A3ED5CC143003A8CA2E91BFCF" originalValue="eg">e-g</normalizedToken>
</figureCitation>
</paragraph>
</subSubSection>
<subSubSection id="F7FC65E27B2F80BB788401A5AEC97003" pageId="0" pageNumber="45" type="holotype">
<paragraph id="D5DFDA6091E930E3DE831D0CA3641E0C" pageId="0" pageNumber="45">Holotype.</paragraph>
<paragraph id="772E00F179675CEFC5831BD7F86E226E" pageId="0" pageNumber="45">
CHINA. Sichuan Province,
<normalizedToken id="35ECCAEEF235728F762ED14EF5C9B914" originalValue="Garzê">Garze</normalizedToken>
Tibetan Autonomous Prefecture, Daocheng County, Yading National Nature Reserve,
<geoCoordinate id="90E818DDE2612B4E959A346EBB6772A5" degrees="28" direction="north" minutes="25" orientation="latitude" precision="15" seconds="6" value="28.418333">28°25'6&quot;N</geoCoordinate>
,
<geoCoordinate id="1B38290558B428030E4DDADAA0EA3198" degrees="100" direction="east" minutes="21" orientation="longitude" precision="15" seconds="26" value="100.35722">100°21'26&quot;E</geoCoordinate>
, alt. 3900 m, on the ground of mixed forest, 18 Aug 2016, J.P. Wang &amp; X.C. Wang 10706, HMAS 254610.
</paragraph>
</subSubSection>
<subSubSection id="F0A917E227BC59F6378B0C39147F3999" pageId="0" pageNumber="45" type="etymology">
<paragraph id="62C897F4FF7411AEC70CDCF80E10F9B1" pageId="0" pageNumber="45">Etymology.</paragraph>
<paragraph id="537B76AAA70EF2A01D44128329AAC044" pageId="0" pageNumber="45">The species epithet refers to the type locality of the fungus.</paragraph>
</subSubSection>
<subSubSection id="1365DB53A1C49604BC6168244C039DE2" pageId="0" pageNumber="45" type="description">
<paragraph id="49D655CECFB2FAFEEB40ADB92EFF8772" pageId="0" pageNumber="45">Description.</paragraph>
<paragraph id="98F36B0542B327F4C5FD239EA9FE626B" pageId="0" pageNumber="45">
Apothecia stipitate, shallow-cupulate, margin entire and flattened when fresh, undulate, involute or revolute when dry, 5-6 cm diam. when fresh and 2.5-3.5 cm high when dry; hymenium yellowish-brown when fresh, nearly black when dry, receptacle surface buff to light brown when fresh, light brown to dark brown when dry, glabrous; stipe terete or flattened, buff to light brown, surface ribbed, 2.5-3
<normalizedToken id="E2EF3D22A3AB671E490CC7F9872ECF55" originalValue="×">x</normalizedToken>
1.5-3 cm when fresh, 2-2.5
<normalizedToken id="39B5C33D51B0358E144018DDC9DE69C6" originalValue="×">x</normalizedToken>
0.5-1.5 cm when dry, typically fluted with sharp-edged or rarely blunt ribs, ribs branching at the upper half of receptacle surface, reaching to the edge or ending 3-5 mm from the edge. Ectal excipulum of
<emphasis id="A9E64B300218AFFC7E250C32EA774B1B" italics="true" pageId="0" pageNumber="45">textura angularis</emphasis>
, 100-180
<normalizedToken id="3A7A5A69355B787084C5AC4EE77541C9" originalValue="µm">µm</normalizedToken>
thick, cells hyaline to light brown, outer cells 15-45
<normalizedToken id="1077C1D87735F5EFE3988E9FB33400A5" originalValue="×">x</normalizedToken>
9-35
<normalizedToken id="4E166EC61D4B89319477E57B94406149" originalValue="µm">µm</normalizedToken>
. Medullary excipulum of
<emphasis id="ED1A0E1E517787668D36116A18A0DF2D" italics="true" pageId="0" pageNumber="45">textura intricata</emphasis>
, 300-500
<normalizedToken id="8FCAFC5D8B9B84C70B106070ADEEAB54" originalValue="µm">µm</normalizedToken>
thick, hyphae hyaline. Asci subcylindrical, tapering and with crozier at base, 8-spored, 225-325
<normalizedToken id="1EB1922E721AFB0EDB5E6EEF93DA9192" originalValue="×">x</normalizedToken>
13-18.5
<normalizedToken id="3EA1559121356751BDE5E4486C2C4297" originalValue="µm">µm</normalizedToken>
. Paraphyses filiform with apical portion obviously swollen, septate, hyaline to light brown, 7-10.5
<normalizedToken id="4EE049BB5235202B5DE5D0876EF612D7" originalValue="µm">µm</normalizedToken>
wide at apex and 3-4.5
<normalizedToken id="D2958F6D006D6C34567B8F3A2F9C294C" originalValue="µm">µm</normalizedToken>
below. Ascospores ellipsoidal, hyaline, smooth, uniguttulate, 15.5-18.5
<normalizedToken id="B5EF354A303C51E703C14D145D5DD79D" originalValue="×">x</normalizedToken>
10-12.5
<normalizedToken id="64FA09D0C0EB7BDA0CD6857DE7E186C1" originalValue="µm">µm</normalizedToken>
, median 16.9
<normalizedToken id="ECBD5A3D7D28586913291C6084A75B68" originalValue="×">x</normalizedToken>
11.2
<normalizedToken id="892F1FF9A655CA2DCBE038FCB4E86134" originalValue="µm">µm</normalizedToken>
, Q = 1.3-1.7, median 1.48, n = 40.
</paragraph>
</subSubSection>
<subSubSection id="11DC580DB7CB22C08756C6FCE115AAEA" pageId="0" pageNumber="45" type="notes">
<paragraph id="97D557C7959233E34F6C4413C1C473E2" pageId="0" pageNumber="45">Notes.</paragraph>
<paragraph id="E438A51691C478CFA90689AEC58EFFEF" pageId="0" pageNumber="45">
<taxonomicName id="193ABDAFE111B9EF06C5879832925868" class="Pezizomycetes" family="Helvellaceae" genus="Helvella" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Helvella sichuanensis" order="Pezizales" pageId="0" pageNumber="45" phylum="Ascomycota" rank="species" species="sichuanensis">
<emphasis id="F3AEA4C486ABF87F87B52D058F5923C0" italics="true" pageId="0" pageNumber="45">Helvella sichuanensis</emphasis>
</taxonomicName>
belongs to Clade 3 (
<figureCitation id="95BC2D5BC22555575628B848821D79FF" captionStart="Figure 1" captionStartId="F1" captionText="Figure 1. Bayesian phylogenetic tree of Helvella and Dissingia inferred from combined Hsp 90, ITS 2, LSU and TEF 1 dataset. Posterior probability values ≥ 0.90 (left) and bootstrap values ≥ 70 % (right) are indicated at nodes." figureDoi="10.3897/mycokeys.60.38186.figure1" httpUri="https://binary.pensoft.net/fig/352437" pageId="0" pageNumber="45" tableDoi="10.3897/mycokeys.60.38186.figure1">Figure 1</figureCitation>
). Its hymenium is nearly black when dry, which is similar to that of
<taxonomicName id="666B9A771B0BF3E057F945DA8969DCDC" lsidName="H. arctoalpina" pageId="0" pageNumber="45" rank="species" species="arctoalpina">
<emphasis id="1E21122D487E439485F34DCAAF824F0D" italics="true" pageId="0" pageNumber="45">H. arctoalpina</emphasis>
</taxonomicName>
, but different from those in
<taxonomicName id="934E611A8A325228A25D1BCD7316ADC5" lsidName="H. acetabulum" pageId="0" pageNumber="45" rank="species" species="acetabulum">
<emphasis id="85035C2CFB94FE9EB9DC1F53EC64C168" italics="true" pageId="0" pageNumber="45">H. acetabulum</emphasis>
</taxonomicName>
(brown when dry) and
<taxonomicName id="EFD595DA12C84F346F9FD41BB42C9516" lsidName="H. acetabuloides" pageId="0" pageNumber="45" rank="species" species="acetabuloides">
<emphasis id="1E15353E81F8387944423B5DAF69FF25" italics="true" pageId="0" pageNumber="45">H. acetabuloides</emphasis>
</taxonomicName>
(reddish-brown when dry). When fresh, the hymenium is yellowish-brown, while that of
<taxonomicName id="BF79FE43698BD2FA3C6B6E1B73BD613D" lsidName="H. arctoalpina" pageId="0" pageNumber="45" rank="species" species="arctoalpina">
<emphasis id="77E0EAA81C09509E64CF455D3EAADFC2" italics="true" pageId="0" pageNumber="45">H. arctoalpina</emphasis>
</taxonomicName>
is brown. Molecularly, it differs from
<taxonomicName id="60741C2B74BD67DF82953531C596103C" lsidName="H. acetabulum" pageId="0" pageNumber="45" rank="species" species="acetabulum">
<emphasis id="3EC6271C567EED23874788F253636E3F" italics="true" pageId="0" pageNumber="45">H. acetabulum</emphasis>
</taxonomicName>
in 7 bp of Hsp90 (H410, epitype), 14 bp of ITS2 (HMAS 243823), 17 bp of LSU (H133) and 15 bp of TEF1 (H133); from
<taxonomicName id="7F95B6B36DFE80811F8597F2CFB6609F" lsidName="H. arctoalpina" pageId="0" pageNumber="45" rank="species" species="arctoalpina">
<emphasis id="298DA58572FDE0502EACD6CC322F55D2" italics="true" pageId="0" pageNumber="45">H. arctoalpina</emphasis>
</taxonomicName>
in 1 bp of Hsp90 (H293, holotype), 25 bp of LSU (H033) and 11 bp of TEF1 (H033); and from
<taxonomicName id="F9010C4CF9260E90379E384034CEC36E" lsidName="H. costata" pageId="0" pageNumber="45" rank="species" species="costata">
<emphasis id="F2EE6CBF25B63F2DBD6B7A89683E8F96" italics="true" pageId="0" pageNumber="45">H. costata</emphasis>
</taxonomicName>
in 2 bp of Hsp90 and 13 bp of LSU. The sequence divergences between
<taxonomicName id="E6002DB6B022EA9C16ABB47343B12F1D" lsidName="H. sichuanensis" pageId="0" pageNumber="45" rank="species" species="sichuanensis">
<emphasis id="E016146327FE29189A84C999AD94D140" italics="true" pageId="0" pageNumber="45">H. sichuanensis</emphasis>
</taxonomicName>
and
<taxonomicName id="57CA0203A3CA8CE98D47A9756BD85380" lsidName="H. acetabuloides" pageId="0" pageNumber="45" rank="species" species="acetabuloides">
<emphasis id="543992801FE7C05733B8CB226C3D3CCF" italics="true" pageId="0" pageNumber="45">H. acetabuloides</emphasis>
</taxonomicName>
are 1 bp of Hsp90, 20 bp of ITS2 and 12 bp of TEF1.
</paragraph>
</subSubSection>
</treatment>
</document>