531 lines
65 KiB
XML
531 lines
65 KiB
XML
<document id="3B23279D77A2B752DF8586AC23E1095F" ID-DOI="10.1016/j.phytochem.2022.113110" ID-ISSN="1873-3700" ID-Zenodo-Dep="8235034" IM.bibliography_approvedBy="felipe" IM.illustrations_approvedBy="carolina" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.tables_approvedBy="carolina" IM.taxonomicNames_approvedBy="carolina" IM.treatments_approvedBy="carolina" checkinTime="1691689278195" checkinUser="felipe" docAuthor="Kruk, Jerzy, Gabruk, Michał, Kryszczak, Marian & Wąsik, Piotr" docDate="2022" docId="220DD551FFACFF80E950EBB0FBC685AB" docLanguage="en" docName="Phytochemistry.197.113110.pdf" docOrigin="Phytochemistry (113110) 197" docSource="http://dx.doi.org/10.1016/j.phytochem.2022.113110" docStyle="DocumentStyle:F36D69FC8B198FBE91029DF9C24697D3.5:Phytochemistry.2020-.journal_article" docStyleId="F36D69FC8B198FBE91029DF9C24697D3" docStyleName="Phytochemistry.2020-.journal_article" docStyleVersion="5" docTitle="Cucurbita Jablonský 1973" docType="treatment" docVersion="4" lastPageNumber="3" masterDocId="DE34AD29FFADFF82EA62E816FFE68356" masterDocTitle="Protochlorophylls in Cucurbitaceae - Distribution, biosynthesis and phylogeny" masterLastPageNumber="8" masterPageNumber="1" pageNumber="2" updateTime="1692299190104" updateUser="ExternalLinkService">
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<mods:title id="B5BD2434D413117640A1779B44B63776">Protochlorophylls in Cucurbitaceae - Distribution, biosynthesis and phylogeny</mods:title>
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<mods:namePart id="A88A131E7A03D8D38B8628A1B7392315">Kruk, Jerzy</mods:namePart>
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<mods:namePart id="69ACB6E666560E1363FF69B0728549B7">Gabruk, Michał</mods:namePart>
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<mods:namePart id="CD3CFC1495C56F8FE1765E44307BB1FD">Kryszczak, Marian</mods:namePart>
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<mods:namePart id="811F4B7FE3733FE1D7C96AE0087FB406">Wąsik, Piotr</mods:namePart>
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<mods:title id="A9F395ED88DF5F5218D718C9B50DF805">Phytochemistry</mods:title>
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<mods:date id="7E0F8E474734AEB1834CF3F38AC574BB">2022</mods:date>
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<subSubSection id="E2BE37CCFFACFF83E950EBB0FB2180EE" box="[818,1223,933,953]" pageId="1" pageNumber="2" type="nomenclature">
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<paragraph id="AA1B6447FFACFF83E950EBB0FB2180EE" blockId="1.[818,1223,933,953]" box="[818,1223,933,953]" pageId="1" pageNumber="2">
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<heading id="F153D32BFFACFF83E950EBB0FB2180EE" bold="true" box="[818,1223,933,953]" fontSize="36" level="1" pageId="1" pageNumber="2" reason="1">
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<emphasis id="98D0B855FFACFF83E950EBB0FB2180EE" bold="true" box="[818,1223,933,953]" italics="true" pageId="1" pageNumber="2">
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2.2. Pigment synthesis in
|
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<taxonomicName id="6DA41FC4FFACFF83EE7FEBB3FB9280EE" authority="Jablonský, 1973" authorityName="Jablonský" authorityYear="1973" box="[1053,1140,933,952]" class="Magnoliopsida" family="Cucurbitaceae" genus="Cucurbita" kingdom="Plantae" order="Cucurbitales" pageId="1" pageNumber="2" phylum="Tracheophyta" rank="species">Cucurbita</taxonomicName>
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seedlings
|
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</emphasis>
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</heading>
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</paragraph>
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</subSubSection>
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<subSubSection id="E2BE37CCFFACFF80E933EBC8FBC685AB" lastPageId="2" lastPageNumber="3" pageId="1" pageNumber="2" type="description">
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<paragraph id="AA1B6447FFACFF80E933EBC8FE208593" blockId="1.[818,1488,990,1344]" lastBlockId="2.[100,770,1240,1956]" lastPageId="2" lastPageNumber="3" pageId="1" pageNumber="2">
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An analysis of porphyrin pigments in etiolated
|
||
<taxonomicName id="6DA41FC4FFACFF83EF46EBC8FA8C80A7" baseAuthorityName="Ellsworth and Nowak" baseAuthorityYear="1974" box="[1316,1386,990,1009]" class="Magnoliopsida" family="Cucurbitaceae" genus="Cucurbita" kingdom="Plantae" order="Cucurbitales" pageId="1" pageNumber="2" phylum="Tracheophyta" rank="species" species="pepo">
|
||
<emphasis id="98D0B855FFACFF83EF46EBC8FA8C80A7" bold="true" box="[1316,1386,990,1009]" italics="true" pageId="1" pageNumber="2">C. pepo</emphasis>
|
||
</taxonomicName>
|
||
‘Miranda’ seedlings revealed that when subjected to continuous illumination (
|
||
<figureCitation id="329F78C2FFACFF83E958EC03FC96877F" box="[826,880,1045,1065]" captionStart="Fig" captionStartId="5.[100,130,1745,1762]" captionTargetBox="[106,796,148,1721]" captionTargetId="figure-7@5.[106,767,148,1718]" captionTargetPageId="5" captionText="Fig. 4. The content of Chl-GG (A), Chl a (B) and Pchl-GG (C) in the primary leaves of 11-day-old etiolated Cucurbita pepo ‘Miranda’ seedlings during illumination. In the case of ALA-treated plants, the 11-day-old seedlings were incubated for additional 3 days in 10 mM ALA solution in the dark before illumination. The data are means ± SE, n = 3–4. FW = fresh weight. The pigments were extracted and analyzed using HPLC as described in the Experimental section." figureDoi="http://doi.org/10.5281/zenodo.8235042" httpUri="https://zenodo.org/record/8235042/files/figure.png" pageId="1" pageNumber="2">Fig. 4</figureCitation>
|
||
), Chl
|
||
<emphasis id="98D0B855FFACFF83E9C9EC00FC50877F" bold="true" box="[939,950,1046,1065]" italics="true" pageId="1" pageNumber="2">a</emphasis>
|
||
content in the control plants increased gradually, as expected, while the level of Chl-GG, a precursor of Chl
|
||
<emphasis id="98D0B855FFACFF83EF42EC27FACD8712" bold="true" box="[1312,1323,1073,1092]" italics="true" pageId="1" pageNumber="2">a</emphasis>
|
||
, was low and did not change significantly during illumination, like that of Pchl-GG. However, when the seedlings were incubated with ALA (precursor of the porphyrin ring) before illumination, the Chl-GG content increased dramatically with illumination time, while the synthesis of Chl
|
||
<emphasis id="98D0B855FFACFF83EFFAECB7FA4587E2" bold="true" box="[1432,1443,1185,1204]" italics="true" pageId="1" pageNumber="2">a</emphasis>
|
||
was inhibited. The synthesis of Pchl-GG was not affected very much (
|
||
<figureCitation id="329F78C2FFACFF83EFEFECABFA278786" box="[1421,1473,1213,1232]" captionStart="Fig" captionStartId="5.[100,130,1745,1762]" captionTargetBox="[106,796,148,1721]" captionTargetId="figure-7@5.[106,767,148,1718]" captionTargetPageId="5" captionText="Fig. 4. The content of Chl-GG (A), Chl a (B) and Pchl-GG (C) in the primary leaves of 11-day-old etiolated Cucurbita pepo ‘Miranda’ seedlings during illumination. In the case of ALA-treated plants, the 11-day-old seedlings were incubated for additional 3 days in 10 mM ALA solution in the dark before illumination. The data are means ± SE, n = 3–4. FW = fresh weight. The pigments were extracted and analyzed using HPLC as described in the Experimental section." figureDoi="http://doi.org/10.5281/zenodo.8235042" httpUri="https://zenodo.org/record/8235042/files/figure.png" pageId="1" pageNumber="2">Fig. 4</figureCitation>
|
||
). It is supposed that Chl
|
||
<emphasis id="98D0B855FFACFF83EE63ECCFFBEA87BA" bold="true" box="[1025,1036,1241,1260]" italics="true" pageId="1" pageNumber="2">a</emphasis>
|
||
is synthesized by Chl synthase from Chlide
|
||
<emphasis id="98D0B855FFACFF83EFFFECCFFA4E87BA" bold="true" box="[1437,1448,1241,1260]" italics="true" pageId="1" pageNumber="2">a</emphasis>
|
||
and both phytyl-PP or GG-PP, depending on the plant species, light conditions (darkness or light), the age of the plants and other factors (
|
||
<bibRefCitation id="CE3519B6FFACFF83EFEBED06FC7D8669" author="Schoefs, B. & Bertrand, M. & Lemoine, Y." pageId="1" pageNumber="2" pagination="203 - 213" refId="ref7800" refString="Schoefs, B., Bertrand, M., Lemoine, Y., 1998. Pigment biosynthesis during the first photoperiod of greening (16 h) of 2 - d-old and 10 - d-old bean leaves. Photosynth. Res. 57, 203 - 213. https: // doi. org / 10.1023 / A: 1006000208160." type="journal article" year="1998">Schoefs et al., 1998</bibRefCitation>
|
||
;
|
||
<bibRefCitation id="CE3519B6FFACFF83E9C5ED3AFB458669" author="Schoefs, B. & Bertrand, M." box="[935,1187,1324,1343]" pageId="1" pageNumber="2" pagination="243 - 246" refId="ref7863" refString="Schoefs, B., Bertrand, M., 2000. The formation of chlorophyll from chlorophyllide in leaves containing proplastids is a four-step process. FEBS Lett. 486, 243 - 246. https: // doi. org / 10.1016 / S 0014 - 5793 (00) 02309 - 7." type="journal article" year="2000">Schoefs and Bertrand 2000</bibRefCitation>
|
||
,
|
||
<bibRefCitation id="CE3519B6FFACFF83EECCED3AFA858669" author="Schmid, H. C. & Oster, U. & Lenz, S. & Rudiger, W." box="[1198,1379,1324,1344]" pageId="1" pageNumber="2" pagination="903 - 911" refId="ref7670" refString="Schmid, H. C., Oster, U., Kogel ¨, J., Lenz, S., Rudiger, W., 2001. Cloning and characterisation of chlorophyll synthase from Avena sativa. Biol. Chem. 382, 903 - 911. https: // doi. org / 10.1515 / BC. 2001.112." type="journal article" year="2001">Schmid et al., 2001</bibRefCitation>
|
||
and
|
||
<bibRefCitation id="CE3519B6FFACFF83EFF3ED3AFA278669" author="Schmid, H. C. & Rassadina, V. & Oster, U. & Schoch, S. & Rudiger, W." box="[1425,1473,1324,1343]" pageId="1" pageNumber="2" pagination="1769 - 1776" refId="ref7733" refString="Schmid, H. C., Rassadina, V., Oster, U., Schoch, S., Rudiger, W., 2002. Pre-loading of chlorophyll synthase with tetraprenyl diphosphate is an obligatory step in chlorophyll biosynthesis. Biol. Chem. 383, 1769 - 1776. https: // doi. org / 10.1515 / BC. 2002.198." type="journal article" year="2002">2002</bibRefCitation>
|
||
). n.d. – not determined. These data suggest that Chl
|
||
<emphasis id="98D0B855FFAFFF80EB08ECCEFE9387BD" bold="true" box="[362,373,1240,1259]" italics="true" pageId="2" pageNumber="3">a</emphasis>
|
||
is not synthesized from GG-PP via Chl-GG but rather directly from phytyl-PP in
|
||
<taxonomicName id="6DA41FC4FFAFFF80EBDBECE2FE1C8651" baseAuthorityName="Ellsworth and Nowak" baseAuthorityYear="1974" box="[441,506,1268,1287]" class="Magnoliopsida" family="Cucurbitaceae" genus="Cucurbita" kingdom="Plantae" order="Cucurbitales" pageId="2" pageNumber="3" phylum="Tracheophyta" rank="species" species="pepo">
|
||
<emphasis id="98D0B855FFAFFF80EBDBECE2FE1C8651" bold="true" box="[441,506,1268,1287]" italics="true" pageId="2" pageNumber="3">C. pepo</emphasis>
|
||
</taxonomicName>
|
||
seedlings. This conclusion is supported by the lack of other Chl
|
||
<emphasis id="98D0B855FFAFFF80EBC8ED06FE538675" bold="true" box="[426,437,1296,1315]" italics="true" pageId="2" pageNumber="3">a</emphasis>
|
||
precursors (Chl-DHGG, Chl-THGG) which are intermediates in the pathway from Chl-GG to Chl
|
||
<emphasis id="98D0B855FFAFFF80E8F9ED3AFD408669" bold="true" box="[667,678,1324,1343]" italics="true" pageId="2" pageNumber="3">a</emphasis>
|
||
(data not shown). The observation that the Chl
|
||
<emphasis id="98D0B855FFAFFF80EBAAED51FE35860C" bold="true" box="[456,467,1351,1370]" italics="true" pageId="2" pageNumber="3">a</emphasis>
|
||
level did not increase after ALA feeding suggests that the phytyl-PP supply is limited. An analysis of the composition of Pchls in primary leaves during illumination (
|
||
<figureCitation id="329F78C2FFAFFF80E8A2ED69FD1C86C4" box="[704,762,1407,1426]" captionStart="Fig" captionStartId="5.[818,848,1774,1791]" captionTargetBox="[826,1484,148,1747]" captionTargetId="figure-96@5.[823,1484,148,1747]" captionText="Fig. 5. Composition of protochlorophylls in primary leaves of 11-day-old etiolated Cucurbita pepo ‘Miranda’ and C. maxima ‘Bambino’ seedlings during illumination. In the case of ALA-treated plants, the 11-day-old seedlings were incubated for additional 3 days in 10 mM ALA solution in the dark before illumination. The data are means ± SE, n = 3–4. The pigments were extracted and analyzed using HPLC as described in the Experimental section." figureDoi="http://doi.org/10.5281/zenodo.8257061" httpUri="https://zenodo.org/record/8257061/files/figure.png" pageId="2" pageNumber="3">Fig. 5</figureCitation>
|
||
) shows that Pchl-GG in the
|
||
<taxonomicName id="6DA41FC4FFAFFF80EB10ED8DFE5E86F8" baseAuthorityName="Ellsworth and Nowak" baseAuthorityYear="1974" box="[370,440,1435,1454]" class="Magnoliopsida" family="Cucurbitaceae" genus="Cucurbita" kingdom="Plantae" order="Cucurbitales" pageId="2" pageNumber="3" phylum="Tracheophyta" rank="species" species="pepo">
|
||
<emphasis id="98D0B855FFAFFF80EB10ED8DFE5E86F8" bold="true" box="[370,440,1435,1454]" italics="true" pageId="2" pageNumber="3">C. pepo</emphasis>
|
||
</taxonomicName>
|
||
‘Miranda’ control plants was the dominating species and the content of individual compounds did not change very much during illumination, apart from Pchl-DHGG whose contribution increased fourfold. In the presence of ALA, a gradual, but not very pronounced, increase in Pchl-GG and Pchl-DHGG levels occurred at the expense of the two other Pchls. These data suggest that the synthesis of Pchls in the leaves of developing seedlings is a relatively slow process, but not limited by the supply of substrates, such as Pchlide (
|
||
<figureCitation id="329F78C2FFAFFF80EA0EEE6CFF4585DB" box="[108,163,1658,1677]" captionStart="Fig" captionStartId="5.[100,130,1745,1762]" captionTargetBox="[106,796,148,1721]" captionTargetId="figure-7@5.[106,767,148,1718]" captionTargetPageId="5" captionText="Fig. 4. The content of Chl-GG (A), Chl a (B) and Pchl-GG (C) in the primary leaves of 11-day-old etiolated Cucurbita pepo ‘Miranda’ seedlings during illumination. In the case of ALA-treated plants, the 11-day-old seedlings were incubated for additional 3 days in 10 mM ALA solution in the dark before illumination. The data are means ± SE, n = 3–4. FW = fresh weight. The pigments were extracted and analyzed using HPLC as described in the Experimental section." figureDoi="http://doi.org/10.5281/zenodo.8235042" httpUri="https://zenodo.org/record/8235042/files/figure.png" pageId="2" pageNumber="3">Fig. 4</figureCitation>
|
||
). It is also probable that the enzyme is only active at the very early stages of the seedlings’ development and its expression decreases quickly with the age of the seedlings.
|
||
</paragraph>
|
||
<caption id="FEDB34CFFFACFF83EA06EF6FFF1E84EB" ID-DOI="http://doi.org/10.5281/zenodo.8235036" ID-Zenodo-Dep="8235036" httpUri="https://zenodo.org/record/8235036/files/figure.png" pageId="1" pageNumber="2" startId="1.[100,130,1913,1930]" targetBox="[341,1246,1392,1883]" targetPageId="1" targetType="figure">
|
||
<paragraph id="AA1B6447FFACFF83EA06EF6FFF1E84EB" blockId="1.[100,1487,1913,1981]" pageId="1" pageNumber="2">
|
||
<emphasis id="98D0B855FFACFF83EA06EF6FFF7884DC" bold="true" box="[100,158,1913,1930]" pageId="1" pageNumber="2">Fig. 1.</emphasis>
|
||
(a) Two-dimensional HPLC chromatogram of protochlorophylls extracted from the seed coat of pumpkin (
|
||
<taxonomicName id="6DA41FC4FFACFF83EE54EF6FFB4984DC" baseAuthorityName="Ellsworth and Nowak" baseAuthorityYear="1974" box="[1078,1199,1913,1930]" class="Magnoliopsida" family="Cucurbitaceae" genus="Cucurbita" kingdom="Plantae" order="Cucurbitales" pageId="1" pageNumber="2" phylum="Tracheophyta" rank="species" species="pepo">
|
||
<emphasis id="98D0B855FFACFF83EE54EF6FFB4984DC" bold="true" box="[1078,1199,1913,1930]" italics="true" pageId="1" pageNumber="2">Cucurbita pepo</emphasis>
|
||
</taxonomicName>
|
||
) seeds and (b) the corresponding one-dimensional chromatogram using absorption detection at 435 nm. Analysis was performed using C30 YMC column, methanol/ethyl acetate (34/16) as eluent and 1.5 ml/min flow.
|
||
</paragraph>
|
||
</caption>
|
||
<caption id="FEDB34CFFFAFFF80EA06E880FCBB8397" ID-Table-UUID="FEDB34CFFFAFFF80EA06E880FCBB8397" httpUri="http://table.plazi.org/id/FEDB34CFFFAFFF80EA06E880FCBB8397" pageId="2" pageNumber="3" startId="2.[100,150,150,166]" targetBox="[116,1471,213,719]" targetIsTable="true" targetPageId="2" targetType="table">
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||
<paragraph id="AA1B6447FFAFFF80EA06E880FF4183F1" blockId="2.[100,861,150,193]" box="[100,167,150,167]" pageId="2" pageNumber="3">
|
||
<emphasis id="98D0B855FFAFFF80EA06E880FF4183F1" bold="true" box="[100,167,150,167]" pageId="2" pageNumber="3">Table 1</emphasis>
|
||
</paragraph>
|
||
<paragraph id="AA1B6447FFAFFF80EA06E8A6FCBB8397" blockId="2.[100,861,150,193]" box="[100,861,176,193]" pageId="2" pageNumber="3">Absorption maxima of porphyrins determined in this study and reported in the literature.</paragraph>
|
||
</caption>
|
||
<paragraph id="AA1B6447FFAFFF80EA16E8C3FAE58199" pageId="2" pageNumber="3">
|
||
<table id="D8A496E7FFAF007DEA16E8C3FA598199" box="[116,1471,213,719]" gridcols="4" gridrows="22" pageId="2" pageNumber="3">
|
||
<tr id="14946605FFAF007DEA16E8C3FA5983B5" box="[116,1471,213,227]" gridrow="0" pageId="2" pageNumber="3">
|
||
<th id="57450F79FFAF007DEA16E8C3FEE083B5" box="[116,262,213,227]" gridcol="0" gridrow="0" pageId="2" pageNumber="3">Compound</th>
|
||
<th id="57450F79FFAF007DEBC2E8C3FDB883B5" box="[416,606,213,227]" gridcol="1" gridrow="0" pageId="2" pageNumber="3">Absorption maxima (nm)</th>
|
||
<th id="57450F79FFAF007DE89AE8C3FBF983B5" box="[760,1055,213,227]" gridcol="2" gridrow="0" pageId="2" pageNumber="3">Solvent</th>
|
||
<th id="57450F79FFAF007DEEDBE8C3FA5983B5" box="[1209,1471,213,227]" gridcol="3" gridrow="0" pageId="2" pageNumber="3">Reference</th>
|
||
</tr>
|
||
<tr id="14946605FFAF007DEA16E8EEFA598250" box="[116,1471,248,262]" gridrow="1" pageId="2" pageNumber="3">
|
||
<th id="57450F79FFAF007DEA16E8EEFEE08250" box="[116,262,248,262]" gridcol="0" gridrow="1" pageId="2" pageNumber="3">
|
||
<emphasis id="98D0B855FFAFFF80EA16E8EEFF268250" bold="true" box="[116,192,248,262]" pageId="2" pageNumber="3">MV-Pchls</emphasis>
|
||
</th>
|
||
<td id="57450F79FFAF007DEBC2E8EEFDB88250" box="[416,606,248,262]" gridcol="1" gridrow="1" pageId="2" pageNumber="3">
|
||
<emphasis id="98D0B855FFAFFF80EBC2E8EEFE018250" bold="true" box="[416,487,248,262]" pageId="2" pageNumber="3">432, 627</emphasis>
|
||
</td>
|
||
<td id="57450F79FFAF007DE89AE8EEFBF98250" box="[760,1055,248,262]" gridcol="2" gridrow="1" pageId="2" pageNumber="3">
|
||
<emphasis id="98D0B855FFAFFF80E89AE8EEFBF98250" bold="true" box="[760,1055,248,262]" pageId="2" pageNumber="3">methanol/ethyl acetate, 34/16 (v/v)</emphasis>
|
||
</td>
|
||
<td id="57450F79FFAF007DEEDBE8EEFA598250" box="[1209,1471,248,262]" gridcol="3" gridrow="1" pageId="2" pageNumber="3">
|
||
<emphasis id="98D0B855FFAFFF80EEDBE8EEFAEF8250" bold="true" box="[1209,1289,248,262]" pageId="2" pageNumber="3">this study</emphasis>
|
||
</td>
|
||
</tr>
|
||
<tr id="14946605FFAF007DEA16E919FA59824B" box="[116,1471,271,285]" gridrow="2" pageId="2" pageNumber="3">
|
||
<th id="57450F79FFAF007DEA16E919FEE0824B" box="[116,262,271,285]" gridcol="0" gridrow="2" pageId="2" pageNumber="3">MV-Pchl</th>
|
||
<td id="57450F79FFAF007DEBC2E919FDB8824B" box="[416,606,271,285]" gridcol="1" gridrow="2" pageId="2" pageNumber="3">432, 622</td>
|
||
<td id="57450F79FFAF007DE89AE919FBF9824B" box="[760,1055,271,285]" gridcol="2" gridrow="2" pageId="2" pageNumber="3">diethyl ether</td>
|
||
<td id="57450F79FFAF007DEEDBE919FA59824B" box="[1209,1471,271,285]" gridcol="3" gridrow="2" pageId="2" pageNumber="3">
|
||
<bibRefCitation id="CE3519B6FFAFFF80EEDBE919FA65824B" author="Houssier, C. & Sauer, K." box="[1209,1411,271,285]" pageId="2" pageNumber="3" pagination="476 - 491" refId="ref7070" refString="Houssier, C., Sauer, K., 1969. Optical properties of the protochlorophyll pigments I. Isolation, characterization, and infrared spectra. Biochim. Biophys. Acta 172, 476 - 491. https: // doi. org / 10.1016 / 0005 - 2728 (69) 90144 - 3." type="journal article" year="1969">Houssier and Sauer (1969)</bibRefCitation>
|
||
,
|
||
</td>
|
||
</tr>
|
||
<tr id="14946605FFAF007DEA16E933FA598265" box="[116,1471,293,307]" gridrow="3" pageId="2" pageNumber="3">
|
||
<th id="57450F79FFAF007DEA16E933FEE08265" box="[116,262,293,307]" gridcol="0" gridrow="3" pageId="2" pageNumber="3">MV-Pchl</th>
|
||
<td id="57450F79FFAF007DEBC2E933FDB88265" box="[416,606,293,307]" gridcol="1" gridrow="3" pageId="2" pageNumber="3">432, 623</td>
|
||
<td id="57450F79FFAF007DE89AE933FBF98265" box="[760,1055,293,307]" gridcol="2" gridrow="3" pageId="2" pageNumber="3">diethyl ether</td>
|
||
<td id="57450F79FFAF007DEEDBE933FA598265" box="[1209,1471,293,307]" gridcol="3" gridrow="3" pageId="2" pageNumber="3">
|
||
<bibRefCitation id="CE3519B6FFAFFF80EEDBE933FA728265" author="Ellsworth, R. K. & Nowak, C. A." box="[1209,1428,293,307]" pageId="2" pageNumber="3" pagination="534 - 546" refId="ref6798" refString="Ellsworth, R. K., Nowak, C. A., 1974. A gas chromatographic-mass spectrometric analysis of the esterifying alcohols of pumpkin seed protochlorophylls. Anal. Biochem. 57, 534 - 546. https: // doi. org / 10.1016 / 0003 - 2697 (74) 90108 - 0." type="journal article" year="1974">Ellsworth and Nowak (1974)</bibRefCitation>
|
||
</td>
|
||
</tr>
|
||
<tr id="14946605FFAF007DEA16E92AFA59821D" box="[116,1471,316,331]" gridrow="4" pageId="2" pageNumber="3">
|
||
<th id="57450F79FFAF007DEA16E92AFEE0821D" box="[116,262,316,331]" gridcol="0" gridrow="4" pageId="2" pageNumber="3">MV-Pchlide</th>
|
||
<td id="57450F79FFAF007DEBC2E92AFDB8821D" box="[416,606,316,331]" gridcol="1" gridrow="4" pageId="2" pageNumber="3">433, 627</td>
|
||
<td id="57450F79FFAF007DE89AE92AFBF9821D" box="[760,1055,316,331]" gridcol="2" gridrow="4" pageId="2" pageNumber="3">methanol/ethyl acetate, 34/16 (v/v)</td>
|
||
<td id="57450F79FFAF007DEEDBE92AFA59821D" box="[1209,1471,316,331]" gridcol="3" gridrow="4" pageId="2" pageNumber="3">this study</td>
|
||
</tr>
|
||
<tr id="14946605FFAF007DEA16E944FA598237" box="[116,1471,338,353]" gridrow="5" pageId="2" pageNumber="3">
|
||
<th id="57450F79FFAF007DEA16E944FEE08237" box="[116,262,338,353]" gridcol="0" gridrow="5" pageId="2" pageNumber="3">MV-Pchlide</th>
|
||
<td id="57450F79FFAF007DEBC2E944FDB88237" box="[416,606,338,353]" gridcol="1" gridrow="5" pageId="2" pageNumber="3">432, 622.5</td>
|
||
<td id="57450F79FFAF007DE89AE944FBF98237" box="[760,1055,338,353]" gridcol="2" gridrow="5" pageId="2" pageNumber="3">diethyl ether</td>
|
||
<td id="57450F79FFAF007DEEDBE944FA598237" box="[1209,1471,338,353]" gridcol="3" gridrow="5" pageId="2" pageNumber="3">Kruk and My´sliwa-Kurdziel (2004)</td>
|
||
</tr>
|
||
<tr id="14946605FFAF007DEA16E97CFA59822E" box="[116,1471,362,376]" gridrow="6" pageId="2" pageNumber="3">
|
||
<th id="57450F79FFAF007DEA16E97CFEE0822E" box="[116,262,362,376]" gridcol="0" gridrow="6" pageId="2" pageNumber="3">
|
||
<emphasis id="98D0B855FFAFFF80EA16E97CFF5B822E" bold="true" box="[116,189,362,376]" pageId="2" pageNumber="3">DV-Pchls</emphasis>
|
||
</th>
|
||
<td id="57450F79FFAF007DEBC2E97CFDB8822E" box="[416,606,362,376]" gridcol="1" gridrow="6" pageId="2" pageNumber="3">
|
||
<emphasis id="98D0B855FFAFFF80EBC2E97CFE01822E" bold="true" box="[416,487,362,376]" pageId="2" pageNumber="3">438, 628</emphasis>
|
||
</td>
|
||
<td id="57450F79FFAF007DE89AE97CFBF9822E" box="[760,1055,362,376]" gridcol="2" gridrow="6" pageId="2" pageNumber="3">
|
||
<emphasis id="98D0B855FFAFFF80E89AE97CFBF9822E" bold="true" box="[760,1055,362,376]" pageId="2" pageNumber="3">methanol/ethyl acetate, 34/16 (v/v)</emphasis>
|
||
</td>
|
||
<td id="57450F79FFAF007DEEDBE97CFA59822E" box="[1209,1471,362,376]" gridcol="3" gridrow="6" pageId="2" pageNumber="3">
|
||
<emphasis id="98D0B855FFAFFF80EEDBE97CFAEF822E" bold="true" box="[1209,1289,362,376]" pageId="2" pageNumber="3">this study</emphasis>
|
||
</td>
|
||
</tr>
|
||
<tr id="14946605FFAF007DEA16E997FA5982D9" box="[116,1471,385,399]" gridrow="7" pageId="2" pageNumber="3">
|
||
<th id="57450F79FFAF007DEA16E997FEE082D9" box="[116,262,385,399]" gridcol="0" gridrow="7" pageId="2" pageNumber="3">DV-Pchl</th>
|
||
<td id="57450F79FFAF007DEBC2E997FDB882D9" box="[416,606,385,399]" gridcol="1" gridrow="7" pageId="2" pageNumber="3">438, 622</td>
|
||
<td id="57450F79FFAF007DE89AE997FBF982D9" box="[760,1055,385,399]" gridcol="2" gridrow="7" pageId="2" pageNumber="3">diethyl ether</td>
|
||
<td id="57450F79FFAF007DEEDBE997FA5982D9" box="[1209,1471,385,399]" gridcol="3" gridrow="7" pageId="2" pageNumber="3">
|
||
<bibRefCitation id="CE3519B6FFAFFF80EEDBE997FA9F82D9" author="Stanier, R. Y. & Smith, J. H. C." box="[1209,1401,385,399]" pageId="2" pageNumber="3" pagination="336 - 338" refId="ref7997" refString="Stanier, R. Y., Smith, J. H. C., 1959. Protochlorophyll from a purple bacterium. Carnegie Inst. Wash. Year Book 58, 336 - 338." type="journal article" year="1959">Stanier and Smith (1959)</bibRefCitation>
|
||
</td>
|
||
</tr>
|
||
<tr id="14946605FFAF007DEA16E98EFA5982F0" box="[116,1471,408,422]" gridrow="8" pageId="2" pageNumber="3">
|
||
<th id="57450F79FFAF007DEA16E98EFEE082F0" box="[116,262,408,422]" gridcol="0" gridrow="8" pageId="2" pageNumber="3">DV-Pchl</th>
|
||
<td id="57450F79FFAF007DEBC2E98EFDB882F0" box="[416,606,408,422]" gridcol="1" gridrow="8" pageId="2" pageNumber="3">437, 622</td>
|
||
<td id="57450F79FFAF007DE89AE98EFBF982F0" box="[760,1055,408,422]" gridcol="2" gridrow="8" pageId="2" pageNumber="3">diethyl ether</td>
|
||
<td id="57450F79FFAF007DEEDBE98EFA5982F0" box="[1209,1471,408,422]" gridcol="3" gridrow="8" pageId="2" pageNumber="3">
|
||
<bibRefCitation id="CE3519B6FFAFFF80EEDBE98EFA6582F0" author="Houssier, C. & Sauer, K." box="[1209,1411,408,422]" pageId="2" pageNumber="3" pagination="476 - 491" refId="ref7070" refString="Houssier, C., Sauer, K., 1969. Optical properties of the protochlorophyll pigments I. Isolation, characterization, and infrared spectra. Biochim. Biophys. Acta 172, 476 - 491. https: // doi. org / 10.1016 / 0005 - 2728 (69) 90144 - 3." type="journal article" year="1969">Houssier and Sauer (1969)</bibRefCitation>
|
||
</td>
|
||
</tr>
|
||
<tr id="14946605FFAF007DEA16E9B8FA5982EB" box="[116,1471,430,445]" gridrow="9" pageId="2" pageNumber="3">
|
||
<th id="57450F79FFAF007DEA16E9B8FEE082EB" box="[116,262,430,445]" gridcol="0" gridrow="9" pageId="2" pageNumber="3">DV-Pchl</th>
|
||
<td id="57450F79FFAF007DEBC2E9B8FDB882EB" box="[416,606,430,445]" gridcol="1" gridrow="9" pageId="2" pageNumber="3">437, 623</td>
|
||
<td id="57450F79FFAF007DE89AE9B8FBF982EB" box="[760,1055,430,445]" gridcol="2" gridrow="9" pageId="2" pageNumber="3">acetonitrile/acetone, 55/45 (v/v)</td>
|
||
<td id="57450F79FFAF007DEEDBE9B8FA5982EB" box="[1209,1471,430,445]" gridcol="3" gridrow="9" pageId="2" pageNumber="3">
|
||
<bibRefCitation id="CE3519B6FFAFFF80EEDBE9B9FAB882EB" author="Mukaida, N. & Kawai, N. & Onoue, Y. & Nishikawa, Y." box="[1209,1374,431,445]" pageId="2" pageNumber="3" pagination="625 - 629" refId="ref7480" refString="Mukaida, N., Kawai, N., Onoue, Y., Nishikawa, Y., 1993. Three-dimensional chromatographic in the inner seed coats of pumpkin. Anal. Sci. 9, 625 - 629. https: // doi. org / 10.2116 / analsci. 9.625." type="journal article" year="1993">Mukaida et al. (1993)</bibRefCitation>
|
||
</td>
|
||
</tr>
|
||
<tr id="14946605FFAF007DEA16E9D3FA598285" box="[116,1471,453,467]" gridrow="10" pageId="2" pageNumber="3">
|
||
<th id="57450F79FFAF007DEA16E9D3FEE08285" box="[116,262,453,467]" gridcol="0" gridrow="10" pageId="2" pageNumber="3">DV-Pchl</th>
|
||
<td id="57450F79FFAF007DEBC2E9D3FDB88285" box="[416,606,453,467]" gridcol="1" gridrow="10" pageId="2" pageNumber="3">436, 622</td>
|
||
<td id="57450F79FFAF007DE89AE9D3FBF98285" box="[760,1055,453,467]" gridcol="2" gridrow="10" pageId="2" pageNumber="3">diethyl ether</td>
|
||
<td id="57450F79FFAF007DEEDBE9D3FA598285" box="[1209,1471,453,467]" gridcol="3" gridrow="10" pageId="2" pageNumber="3">
|
||
<bibRefCitation id="CE3519B6FFAFFF80EEDBE9D3FA4E8285" author="Mukaida, N. & Nishikawa, Y." box="[1209,1448,453,467]" pageId="2" pageNumber="3" pagination="1244 - 1249" refId="ref7535" refString="Mukaida, N., Nishikawa, Y., 1990. Chromatographic separation of protochlorophylls and the structural analysis by fast atom bombardment mass spectrometry. Nippon Kagaku Kaishi 1990, 1244 - 1249." type="journal article" year="1990">Mukaida and Nishikawa (1990)</bibRefCitation>
|
||
</td>
|
||
</tr>
|
||
<tr id="14946605FFAF007DEA16E9CAFA5982BD" box="[116,1471,476,491]" gridrow="11" pageId="2" pageNumber="3">
|
||
<th id="57450F79FFAF007DEA16E9CAFEE082BD" box="[116,262,476,491]" gridcol="0" gridrow="11" pageId="2" pageNumber="3">DV-Pchlide</th>
|
||
<td id="57450F79FFAF007DEBC2E9CAFDB882BD" box="[416,606,476,491]" gridcol="1" gridrow="11" pageId="2" pageNumber="3">437, 627.5</td>
|
||
<td id="57450F79FFAF007DE89AE9CAFBF982BD" box="[760,1055,476,491]" gridcol="2" gridrow="11" pageId="2" pageNumber="3">methanol/ethyl acetate, 34/16 (v/v)</td>
|
||
<td id="57450F79FFAF007DEEDBE9CAFA5982BD" box="[1209,1471,476,491]" gridcol="3" gridrow="11" pageId="2" pageNumber="3">this study</td>
|
||
</tr>
|
||
<tr id="14946605FFAF007DEA16E9E7FA598157" box="[116,1471,497,513]" gridrow="12" pageId="2" pageNumber="3">
|
||
<th id="57450F79FFAF007DEA16E9E7FEE08157" box="[116,262,497,513]" gridcol="0" gridrow="12" pageId="2" pageNumber="3">DV-Pchlide</th>
|
||
<td id="57450F79FFAF007DEBC2E9E7FDB88157" box="[416,606,497,513]" gridcol="1" gridrow="12" pageId="2" pageNumber="3">436.5, 623</td>
|
||
<td id="57450F79FFAF007DE89AE9E7FBF98157" box="[760,1055,497,513]" gridcol="2" gridrow="12" pageId="2" pageNumber="3">diethyl ether</td>
|
||
<td id="57450F79FFAF007DEEDBE9E7FA598157" box="[1209,1471,497,513]" gridcol="3" gridrow="12" pageId="2" pageNumber="3">Kruk and My´sliwa-Kurdziel (2004)</td>
|
||
</tr>
|
||
<tr id="14946605FFAF007DEA16EA1CFA59814E" box="[116,1471,522,536]" gridrow="13" pageId="2" pageNumber="3">
|
||
<th id="57450F79FFAF007DEA16EA1CFEE0814E" box="[116,262,522,536]" gridcol="0" gridrow="13" pageId="2" pageNumber="3">
|
||
<emphasis id="98D0B855FFAFFF80EA16EA1CFF29814E" bold="true" box="[116,207,522,536]" pageId="2" pageNumber="3">MV-Ppheos</emphasis>
|
||
</th>
|
||
<td id="57450F79FFAF007DEBC2EA1CFDB8814E" box="[416,606,522,536]" gridcol="1" gridrow="13" pageId="2" pageNumber="3">
|
||
<emphasis id="98D0B855FFAFFF80EBC2EA1CFE17814E" bold="true" box="[416,497,522,536]" pageId="2" pageNumber="3">418, n.d.*</emphasis>
|
||
</td>
|
||
<td id="57450F79FFAF007DE89AEA1CFBF9814E" box="[760,1055,522,536]" gridcol="2" gridrow="13" pageId="2" pageNumber="3">
|
||
<emphasis id="98D0B855FFAFFF80E89AEA1CFC1B814E" bold="true" box="[760,1021,522,536]" pageId="2" pageNumber="3">methanol/acetonitrile, 1:1 (v/v)</emphasis>
|
||
</td>
|
||
<td id="57450F79FFAF007DEEDBEA1CFA59814E" box="[1209,1471,522,536]" gridcol="3" gridrow="13" pageId="2" pageNumber="3">
|
||
<emphasis id="98D0B855FFAFFF80EEDBEA1CFAEF814E" bold="true" box="[1209,1289,522,536]" pageId="2" pageNumber="3">this study</emphasis>
|
||
</td>
|
||
</tr>
|
||
<tr id="14946605FFAF007DEA16EA37FA598179" box="[116,1471,545,559]" gridrow="14" pageId="2" pageNumber="3">
|
||
<th id="57450F79FFAF007DEA16EA37FEE08179" box="[116,262,545,559]" gridcol="0" gridrow="14" pageId="2" pageNumber="3">MV-Ppheo</th>
|
||
<td id="57450F79FFAF007DEBC2EA37FDB88179" box="[416,606,545,559]" gridcol="1" gridrow="14" pageId="2" pageNumber="3">417, 567, 588</td>
|
||
<td id="57450F79FFAF007DE89AEA37FBF98179" box="[760,1055,545,559]" gridcol="2" gridrow="14" pageId="2" pageNumber="3">acetonitrile/acetone, 55/45 (v/v)</td>
|
||
<td id="57450F79FFAF007DEEDBEA37FA598179" box="[1209,1471,545,559]" gridcol="3" gridrow="14" pageId="2" pageNumber="3">
|
||
<bibRefCitation id="CE3519B6FFAFFF80EEDBEA37FAB88179" author="Mukaida, N. & Kawai, N. & Onoue, Y. & Nishikawa, Y." box="[1209,1374,545,559]" pageId="2" pageNumber="3" pagination="625 - 629" refId="ref7480" refString="Mukaida, N., Kawai, N., Onoue, Y., Nishikawa, Y., 1993. Three-dimensional chromatographic in the inner seed coats of pumpkin. Anal. Sci. 9, 625 - 629. https: // doi. org / 10.2116 / analsci. 9.625." type="journal article" year="1993">Mukaida et al. (1993)</bibRefCitation>
|
||
</td>
|
||
</tr>
|
||
<tr id="14946605FFAF007DEA16EA2EFA598110" box="[116,1471,568,582]" gridrow="15" pageId="2" pageNumber="3">
|
||
<th id="57450F79FFAF007DEA16EA2EFEE08110" box="[116,262,568,582]" gridcol="0" gridrow="15" pageId="2" pageNumber="3">MV-Ppheo</th>
|
||
<td id="57450F79FFAF007DEBC2EA2EFDB88110" box="[416,606,568,582]" gridcol="1" gridrow="15" pageId="2" pageNumber="3">418, 565, 588</td>
|
||
<td id="57450F79FFAF007DE89AEA2EFBF98110" box="[760,1055,568,582]" gridcol="2" gridrow="15" pageId="2" pageNumber="3">diethyl ether</td>
|
||
<td id="57450F79FFAF007DEEDBEA2EFA598110" box="[1209,1471,568,582]" gridcol="3" gridrow="15" pageId="2" pageNumber="3">
|
||
<bibRefCitation id="CE3519B6FFAFFF80EEDBEA2EFA658110" author="Houssier, C. & Sauer, K." box="[1209,1411,568,582]" pageId="2" pageNumber="3" pagination="476 - 491" refId="ref7070" refString="Houssier, C., Sauer, K., 1969. Optical properties of the protochlorophyll pigments I. Isolation, characterization, and infrared spectra. Biochim. Biophys. Acta 172, 476 - 491. https: // doi. org / 10.1016 / 0005 - 2728 (69) 90144 - 3." type="journal article" year="1969">Houssier and Sauer (1969)</bibRefCitation>
|
||
</td>
|
||
</tr>
|
||
<tr id="14946605FFAF007DEA16EA58FA59810B" box="[116,1471,590,605]" gridrow="16" pageId="2" pageNumber="3">
|
||
<th id="57450F79FFAF007DEA16EA58FEE0810B" box="[116,262,590,605]" gridcol="0" gridrow="16" pageId="2" pageNumber="3">MV-Ppheophorbide</th>
|
||
<td id="57450F79FFAF007DEBC2EA58FDB8810B" box="[416,606,590,605]" gridcol="1" gridrow="16" pageId="2" pageNumber="3">418, 563, 597.5</td>
|
||
<td id="57450F79FFAF007DE89AEA58FBF9810B" box="[760,1055,590,605]" gridcol="2" gridrow="16" pageId="2" pageNumber="3">methanol/acetonitrile, 1:1 (v/v)</td>
|
||
<td id="57450F79FFAF007DEEDBEA58FA59810B" box="[1209,1471,590,605]" gridcol="3" gridrow="16" pageId="2" pageNumber="3">this study</td>
|
||
</tr>
|
||
<tr id="14946605FFAF007DEA16EA73FA598125" box="[116,1471,613,627]" gridrow="17" pageId="2" pageNumber="3">
|
||
<th id="57450F79FFAF007DEA16EA73FEE08125" box="[116,262,613,627]" gridcol="0" gridrow="17" pageId="2" pageNumber="3">
|
||
<emphasis id="98D0B855FFAFFF80EA16EA73FF2A8125" bold="true" box="[116,204,613,627]" pageId="2" pageNumber="3">DV-Ppheos</emphasis>
|
||
</th>
|
||
<td id="57450F79FFAF007DEBC2EA73FDB88125" box="[416,606,613,627]" gridcol="1" gridrow="17" pageId="2" pageNumber="3">
|
||
<emphasis id="98D0B855FFAFFF80EBC2EA73FE0F8125" bold="true" box="[416,489,613,627]" pageId="2" pageNumber="3">422, n.d.</emphasis>
|
||
</td>
|
||
<td id="57450F79FFAF007DE89AEA73FBF98125" box="[760,1055,613,627]" gridcol="2" gridrow="17" pageId="2" pageNumber="3">
|
||
<emphasis id="98D0B855FFAFFF80E89AEA73FC1B8125" bold="true" box="[760,1021,613,627]" pageId="2" pageNumber="3">methanol/acetonitrile, 1:1 (v/v)</emphasis>
|
||
</td>
|
||
<td id="57450F79FFAF007DEEDBEA73FA598125" box="[1209,1471,613,627]" gridcol="3" gridrow="17" pageId="2" pageNumber="3">
|
||
<emphasis id="98D0B855FFAFFF80EEDBEA73FAEF8125" bold="true" box="[1209,1289,613,627]" pageId="2" pageNumber="3">this study</emphasis>
|
||
</td>
|
||
</tr>
|
||
<tr id="14946605FFAF007DEA16EA6AFA5981DC" box="[116,1471,636,650]" gridrow="18" pageId="2" pageNumber="3">
|
||
<th id="57450F79FFAF007DEA16EA6AFEE081DC" box="[116,262,636,650]" gridcol="0" gridrow="18" pageId="2" pageNumber="3">DV-Ppheo</th>
|
||
<td id="57450F79FFAF007DEBC2EA6AFDB881DC" box="[416,606,636,650]" gridcol="1" gridrow="18" pageId="2" pageNumber="3">421, 569, 591</td>
|
||
<td id="57450F79FFAF007DE89AEA6AFBF981DC" box="[760,1055,636,650]" gridcol="2" gridrow="18" pageId="2" pageNumber="3">acetonitrile/acetone, 55/45 (v/v)</td>
|
||
<td id="57450F79FFAF007DEEDBEA6AFA5981DC" box="[1209,1471,636,650]" gridcol="3" gridrow="18" pageId="2" pageNumber="3">
|
||
<bibRefCitation id="CE3519B6FFAFFF80EEDBEA6AFAB881DC" author="Mukaida, N. & Kawai, N. & Onoue, Y. & Nishikawa, Y." box="[1209,1374,636,650]" pageId="2" pageNumber="3" pagination="625 - 629" refId="ref7480" refString="Mukaida, N., Kawai, N., Onoue, Y., Nishikawa, Y., 1993. Three-dimensional chromatographic in the inner seed coats of pumpkin. Anal. Sci. 9, 625 - 629. https: // doi. org / 10.2116 / analsci. 9.625." type="journal article" year="1993">Mukaida et al. (1993)</bibRefCitation>
|
||
</td>
|
||
</tr>
|
||
<tr id="14946605FFAF007DEA16EA85FA5981F7" box="[116,1471,659,673]" gridrow="19" pageId="2" pageNumber="3">
|
||
<th id="57450F79FFAF007DEA16EA85FEE081F7" box="[116,262,659,673]" gridcol="0" gridrow="19" pageId="2" pageNumber="3">DV-Ppheo</th>
|
||
<td id="57450F79FFAF007DEBC2EA85FDB881F7" box="[416,606,659,673]" gridcol="1" gridrow="19" pageId="2" pageNumber="3">421, 566, 587</td>
|
||
<td id="57450F79FFAF007DE89AEA85FBF981F7" box="[760,1055,659,673]" gridcol="2" gridrow="19" pageId="2" pageNumber="3">diethyl ether</td>
|
||
<td id="57450F79FFAF007DEEDBEA85FA5981F7" box="[1209,1471,659,673]" gridcol="3" gridrow="19" pageId="2" pageNumber="3">
|
||
<bibRefCitation id="CE3519B6FFAFFF80EEDBEA85FA4E81F7" author="Mukaida, N. & Nishikawa, Y." box="[1209,1448,659,673]" pageId="2" pageNumber="3" pagination="1244 - 1249" refId="ref7535" refString="Mukaida, N., Nishikawa, Y., 1990. Chromatographic separation of protochlorophylls and the structural analysis by fast atom bombardment mass spectrometry. Nippon Kagaku Kaishi 1990, 1244 - 1249." type="journal article" year="1990">Mukaida and Nishikawa (1990)</bibRefCitation>
|
||
</td>
|
||
</tr>
|
||
<tr id="14946605FFAF007DEA16EABCFA5981EE" box="[116,1471,682,696]" gridrow="20" pageId="2" pageNumber="3">
|
||
<th id="57450F79FFAF007DEA16EABCFEE081EE" box="[116,262,682,696]" gridcol="0" gridrow="20" pageId="2" pageNumber="3">DV-Ppheo</th>
|
||
<td id="57450F79FFAF007DEBC2EABCFDB881EE" box="[416,606,682,696]" gridcol="1" gridrow="20" pageId="2" pageNumber="3">421.5, 567, 591</td>
|
||
<td id="57450F79FFAF007DE89AEABCFBF981EE" box="[760,1055,682,696]" gridcol="2" gridrow="20" pageId="2" pageNumber="3">diethyl ether</td>
|
||
<td id="57450F79FFAF007DEEDBEABCFA5981EE" box="[1209,1471,682,696]" gridcol="3" gridrow="20" pageId="2" pageNumber="3">
|
||
<bibRefCitation id="CE3519B6FFAFFF80EEDBEABCFAFA81EE" author="Jones, O. T. G." box="[1209,1308,682,696]" pageId="2" pageNumber="3" pagination="153 - 160" refId="ref7128" refString="Jones, O. T. G., 1966. A protein-protochlorophyll complex obtained from inner seed coats of Cucurbita pepo. Biochem. J. 101, 153 - 160. https: // doi. org / 10.1042 / bj 1010153." type="journal article" year="1966">Jones (1966)</bibRefCitation>
|
||
</td>
|
||
</tr>
|
||
<tr id="14946605FFAF007DEA16EAD7FA598199" box="[116,1471,705,719]" gridrow="21" pageId="2" pageNumber="3">
|
||
<th id="57450F79FFAF007DEA16EAD7FEE08199" box="[116,262,705,719]" gridcol="0" gridrow="21" pageId="2" pageNumber="3">DV-Ppheophorbide</th>
|
||
<td id="57450F79FFAF007DEBC2EAD7FDB88199" box="[416,606,705,719]" gridcol="1" gridrow="21" pageId="2" pageNumber="3">421, 566.5, 597.5</td>
|
||
<td id="57450F79FFAF007DE89AEAD7FBF98199" box="[760,1055,705,719]" gridcol="2" gridrow="21" pageId="2" pageNumber="3">methanol/acetonitrile, 1:1 (v/v)</td>
|
||
<td id="57450F79FFAF007DEEDBEAD7FA598199" box="[1209,1471,705,719]" gridcol="3" gridrow="21" pageId="2" pageNumber="3">this study</td>
|
||
</tr>
|
||
</table>
|
||
</paragraph>
|
||
<caption id="FEDB34CFFFAFFF80EA06EB27FB7C8023" ID-Table-UUID="FEDB34CFFFAFFF80EA06EB27FB7C8023" httpUri="http://table.plazi.org/id/FEDB34CFFFAFFF80EA06EB27FB7C8023" pageId="2" pageNumber="3" startId="2.[100,150,817,833]" targetBox="[116,1471,905,1160]" targetIsTable="true" targetPageId="2" targetType="table">
|
||
<paragraph id="AA1B6447FFAFFF80EA06EB27FF418014" blockId="2.[100,1487,817,885]" box="[100,167,817,834]" pageId="2" pageNumber="3">
|
||
<emphasis id="98D0B855FFAFFF80EA06EB27FF418014" bold="true" box="[100,167,817,834]" pageId="2" pageNumber="3">Table 2</emphasis>
|
||
</paragraph>
|
||
<paragraph id="AA1B6447FFAFFF80EA06EB5DFB7C8023" blockId="2.[100,1487,817,885]" pageId="2" pageNumber="3">
|
||
Composition of protochlorophylls in the seed coats of the species and varieties of
|
||
<taxonomicName id="6DA41FC4FFAFFF80E956EB5DFC4A800A" box="[820,940,843,860]" class="Magnoliopsida" family="Cucurbitaceae" kingdom="Plantae" order="Cucurbitales" pageId="2" pageNumber="3" phylum="Tracheophyta" rank="family">Cucurbitaceae</taxonomicName>
|
||
under investigation. The compounds are ordered according to increasing retention times in HPLC chromatograms. The data are the means of 3 replicates with SE ≤ 20% of the mean values.
|
||
</paragraph>
|
||
</caption>
|
||
<paragraph id="AA1B6447FFAFFF80EA16EB9FFA6C87DE" pageId="2" pageNumber="3">
|
||
<table id="D8A496E7FFAF007DEA16EB9FFA5987DE" box="[116,1471,905,1160]" gridcols="9" gridrows="10" pageId="2" pageNumber="3">
|
||
<tr id="14946605FFAF007DEA16EB9FFA5980C1" box="[116,1471,905,919]" gridrow="0" pageId="2" pageNumber="3">
|
||
<th id="57450F79FFAF007DEA16EB9FFEA480C1" box="[116,322,905,919]" gridcol="0" gridrow="0" pageId="2" pageNumber="3">Protochlorophylls (% total)</th>
|
||
<th id="57450F79FFAF007DEBE8EB9FFE2780C1" box="[394,449,905,919]" gridcol="1" gridrow="0" pageId="2" pageNumber="3">MV-GG</th>
|
||
<th id="57450F79FFAF007DE86BEB9FFDBF80C1" box="[521,601,905,919]" gridcol="2" gridrow="0" pageId="2" pageNumber="3">MV-DHGG</th>
|
||
<th id="57450F79FFAF007DE8C3EB9FFD0980C1" box="[673,751,905,919]" gridcol="3" gridrow="0" pageId="2" pageNumber="3">MV-THGG</th>
|
||
<th id="57450F79FFAF007DE955EB9FFC6180C1" box="[823,903,905,919]" gridcol="4" gridrow="0" pageId="2" pageNumber="3">MV-phytol</th>
|
||
<th id="57450F79FFAF007DE9ADEB9FFBE580C1" box="[975,1027,905,919]" gridcol="5" gridrow="0" pageId="2" pageNumber="3">DV-GG</th>
|
||
<th id="57450F79FFAF007DEE29EB9FFB7E80C1" box="[1099,1176,905,919]" gridcol="6" gridrow="0" pageId="2" pageNumber="3">DV-DHGG</th>
|
||
<th id="57450F79FFAF007DEEBDEB9FFACC80C1" box="[1247,1322,905,919]" gridcol="7" gridrow="0" pageId="2" pageNumber="3">DV-THGG</th>
|
||
<th id="57450F79FFAF007DEF10EB9FFA5980C1" box="[1394,1471,905,919]" gridcol="8" gridrow="0" pageId="2" pageNumber="3">DV-phytol</th>
|
||
</tr>
|
||
<tr id="14946605FFAF007DEA16EBBAFA5980EC" box="[116,1471,940,954]" gridrow="1" pageId="2" pageNumber="3">
|
||
<th id="57450F79FFAF007DEA16EBBAFEA480EC" box="[116,322,940,954]" gridcol="0" gridrow="1" pageId="2" pageNumber="3">Log k’</th>
|
||
<td id="57450F79FFAF007DEBE8EBBAFE2780EC" box="[394,449,940,954]" gridcol="1" gridrow="1" pageId="2" pageNumber="3">1.204</td>
|
||
<td id="57450F79FFAF007DE86BEBBAFDBF80EC" box="[521,601,940,954]" gridcol="2" gridrow="1" pageId="2" pageNumber="3">1.234</td>
|
||
<td id="57450F79FFAF007DE8C3EBBAFD0980EC" box="[673,751,940,954]" gridcol="3" gridrow="1" pageId="2" pageNumber="3">1.273</td>
|
||
<td id="57450F79FFAF007DE955EBBAFC6180EC" box="[823,903,940,954]" gridcol="4" gridrow="1" pageId="2" pageNumber="3">1.335</td>
|
||
<td id="57450F79FFAF007DE9ADEBBAFBE580EC" box="[975,1027,940,954]" gridcol="5" gridrow="1" pageId="2" pageNumber="3">1.396</td>
|
||
<td id="57450F79FFAF007DEE29EBBAFB7E80EC" box="[1099,1176,940,954]" gridcol="6" gridrow="1" pageId="2" pageNumber="3">1.425</td>
|
||
<td id="57450F79FFAF007DEEBDEBBAFACC80EC" box="[1247,1322,940,954]" gridcol="7" gridrow="1" pageId="2" pageNumber="3">1.463</td>
|
||
<td id="57450F79FFAF007DEF10EBBAFA5980EC" box="[1394,1471,940,954]" gridcol="8" gridrow="1" pageId="2" pageNumber="3">1.528</td>
|
||
</tr>
|
||
<tr id="14946605FFAF007DEA16EBD5FA598087" box="[116,1471,963,977]" gridrow="2" pageId="2" pageNumber="3">
|
||
<th id="57450F79FFAF007DEA16EBD5FEA48087" box="[116,322,963,977]" gridcol="0" gridrow="2" pageId="2" pageNumber="3">
|
||
<taxonomicName id="6DA41FC4FFAFFF80EA16EBD5FF068087" baseAuthorityName="Ellsworth and Nowak" baseAuthorityYear="1974" box="[116,224,963,977]" class="Magnoliopsida" family="Cucurbitaceae" genus="Cucurbita" kingdom="Plantae" order="Cucurbitales" pageId="2" pageNumber="3" phylum="Tracheophyta" rank="species" species="pepo">
|
||
<emphasis id="98D0B855FFAFFF80EA16EBD5FF068087" bold="true" box="[116,224,963,977]" italics="true" pageId="2" pageNumber="3">Cucurbita pepo</emphasis>
|
||
</taxonomicName>
|
||
</th>
|
||
<td id="57450F79FFAF007DEBE8EBD5FE278087" box="[394,449,963,977]" gridcol="1" gridrow="2" pageId="2" pageNumber="3">11.3</td>
|
||
<td id="57450F79FFAF007DE86BEBD5FDBF8087" box="[521,601,963,977]" gridcol="2" gridrow="2" pageId="2" pageNumber="3">10.8</td>
|
||
<td id="57450F79FFAF007DE8C3EBD5FD098087" box="[673,751,963,977]" gridcol="3" gridrow="2" pageId="2" pageNumber="3">10.6</td>
|
||
<td id="57450F79FFAF007DE955EBD5FC618087" box="[823,903,963,977]" gridcol="4" gridrow="2" pageId="2" pageNumber="3">8.3</td>
|
||
<td id="57450F79FFAF007DE9ADEBD5FBE58087" box="[975,1027,963,977]" gridcol="5" gridrow="2" pageId="2" pageNumber="3">16.7</td>
|
||
<td id="57450F79FFAF007DEE29EBD5FB7E8087" box="[1099,1176,963,977]" gridcol="6" gridrow="2" pageId="2" pageNumber="3">12.5</td>
|
||
<td id="57450F79FFAF007DEEBDEBD5FACC8087" box="[1247,1322,963,977]" gridcol="7" gridrow="2" pageId="2" pageNumber="3">11.7</td>
|
||
<td id="57450F79FFAF007DEF10EBD5FA598087" box="[1394,1471,963,977]" gridcol="8" gridrow="2" pageId="2" pageNumber="3">17.9</td>
|
||
</tr>
|
||
<tr id="14946605FFAF007DEA16EBCCFA5980BE" box="[116,1471,986,1000]" gridrow="3" pageId="2" pageNumber="3">
|
||
<th id="57450F79FFAF007DEA16EBCCFEA480BE" box="[116,322,986,1000]" gridcol="0" gridrow="3" pageId="2" pageNumber="3">
|
||
<taxonomicName id="6DA41FC4FFAFFF80EA16EBCCFF1F80BE" box="[116,249,986,1000]" class="Magnoliopsida" family="Cucurbitaceae" genus="Cucurbita" kingdom="Plantae" order="Cucurbitales" pageId="2" pageNumber="3" phylum="Tracheophyta" rank="species" species="maxima">
|
||
<emphasis id="98D0B855FFAFFF80EA16EBCCFF1F80BE" bold="true" box="[116,249,986,1000]" italics="true" pageId="2" pageNumber="3">Cucurbita maxima</emphasis>
|
||
</taxonomicName>
|
||
</th>
|
||
<td id="57450F79FFAF007DEBE8EBCCFE2780BE" box="[394,449,986,1000]" gridcol="1" gridrow="3" pageId="2" pageNumber="3">14.9</td>
|
||
<td id="57450F79FFAF007DE86BEBCCFDBF80BE" box="[521,601,986,1000]" gridcol="2" gridrow="3" pageId="2" pageNumber="3">12.7</td>
|
||
<td id="57450F79FFAF007DE8C3EBCCFD0980BE" box="[673,751,986,1000]" gridcol="3" gridrow="3" pageId="2" pageNumber="3">15.7</td>
|
||
<td id="57450F79FFAF007DE955EBCCFC6180BE" box="[823,903,986,1000]" gridcol="4" gridrow="3" pageId="2" pageNumber="3">2.2</td>
|
||
<td id="57450F79FFAF007DE9ADEBCCFBE580BE" box="[975,1027,986,1000]" gridcol="5" gridrow="3" pageId="2" pageNumber="3">20.5</td>
|
||
<td id="57450F79FFAF007DEE29EBCCFB7E80BE" box="[1099,1176,986,1000]" gridcol="6" gridrow="3" pageId="2" pageNumber="3">13.9</td>
|
||
<td id="57450F79FFAF007DEEBDEBCCFACC80BE" box="[1247,1322,986,1000]" gridcol="7" gridrow="3" pageId="2" pageNumber="3">17.8</td>
|
||
<td id="57450F79FFAF007DEF10EBCCFA5980BE" box="[1394,1471,986,1000]" gridcol="8" gridrow="3" pageId="2" pageNumber="3">2.2</td>
|
||
</tr>
|
||
<tr id="14946605FFAF007DEA16EBE7FA598740" box="[116,1471,1009,1046]" gridrow="4" pageId="2" pageNumber="3">
|
||
<th id="57450F79FFAF007DEA16EBE7FEA48740" box="[116,322,1009,1046]" gridcol="0" gridrow="4" pageId="2" pageNumber="3">
|
||
‘Melon Yellow’
|
||
<taxonomicName id="6DA41FC4FFAFFF80EA16EC11FF1F8743" box="[116,249,1031,1045]" class="Magnoliopsida" family="Cucurbitaceae" genus="Cucurbita" kingdom="Plantae" order="Cucurbitales" pageId="2" pageNumber="3" phylum="Tracheophyta" rank="species" species="maxima">
|
||
<emphasis id="98D0B855FFAFFF80EA16EC11FF1F8743" bold="true" box="[116,249,1031,1045]" italics="true" pageId="2" pageNumber="3">Cucurbita maxima</emphasis>
|
||
</taxonomicName>
|
||
a
|
||
</th>
|
||
<td id="57450F79FFAF007DEBE8EBE7FE278740" box="[394,449,1009,1046]" gridcol="1" gridrow="4" pageId="2" pageNumber="3">9.6</td>
|
||
<td id="57450F79FFAF007DE86BEBE7FDBF8740" box="[521,601,1009,1046]" gridcol="2" gridrow="4" pageId="2" pageNumber="3">9.5</td>
|
||
<td id="57450F79FFAF007DE8C3EBE7FD098740" box="[673,751,1009,1046]" gridcol="3" gridrow="4" pageId="2" pageNumber="3">13.3</td>
|
||
<td id="57450F79FFAF007DE955EBE7FC618740" box="[823,903,1009,1046]" gridcol="4" gridrow="4" pageId="2" pageNumber="3">0.6</td>
|
||
<td id="57450F79FFAF007DE9ADEBE7FBE58740" box="[975,1027,1009,1046]" gridcol="5" gridrow="4" pageId="2" pageNumber="3">21.9</td>
|
||
<td id="57450F79FFAF007DEE29EBE7FB7E8740" box="[1099,1176,1009,1046]" gridcol="6" gridrow="4" pageId="2" pageNumber="3">18.4</td>
|
||
<td id="57450F79FFAF007DEEBDEBE7FACC8740" box="[1247,1322,1009,1046]" gridcol="7" gridrow="4" pageId="2" pageNumber="3">25.2</td>
|
||
<td id="57450F79FFAF007DEF10EBE7FA598740" box="[1394,1471,1009,1046]" gridcol="8" gridrow="4" pageId="2" pageNumber="3">1.4</td>
|
||
</tr>
|
||
<tr id="14946605FFAF007DEA16EC08FA59877B" box="[116,1471,1054,1069]" gridrow="5" pageId="2" pageNumber="3">
|
||
<th id="57450F79FFAF007DEA16EC08FEA4877B" box="[116,322,1054,1069]" gridcol="0" gridrow="5" pageId="2" pageNumber="3">Marrow</th>
|
||
<td id="57450F79FFAF007DEBE8EC08FE27877B" box="[394,449,1054,1069]" gridcol="1" gridrow="5" pageId="2" pageNumber="3">7.5</td>
|
||
<td id="57450F79FFAF007DE86BEC08FDBF877B" box="[521,601,1054,1069]" gridcol="2" gridrow="5" pageId="2" pageNumber="3">4.5</td>
|
||
<td id="57450F79FFAF007DE8C3EC08FD09877B" box="[673,751,1054,1069]" gridcol="3" gridrow="5" pageId="2" pageNumber="3">15.7</td>
|
||
<td id="57450F79FFAF007DE955EC08FC61877B" box="[823,903,1054,1069]" gridcol="4" gridrow="5" pageId="2" pageNumber="3">18.4</td>
|
||
<td id="57450F79FFAF007DE9ADEC08FBE5877B" box="[975,1027,1054,1069]" gridcol="5" gridrow="5" pageId="2" pageNumber="3">8.4</td>
|
||
<td id="57450F79FFAF007DEE29EC08FB7E877B" box="[1099,1176,1054,1069]" gridcol="6" gridrow="5" pageId="2" pageNumber="3">3.0</td>
|
||
<td id="57450F79FFAF007DEEBDEC08FACC877B" box="[1247,1322,1054,1069]" gridcol="7" gridrow="5" pageId="2" pageNumber="3">17.9</td>
|
||
<td id="57450F79FFAF007DEF10EC08FA59877B" box="[1394,1471,1054,1069]" gridcol="8" gridrow="5" pageId="2" pageNumber="3">24.1</td>
|
||
</tr>
|
||
<tr id="14946605FFAF007DEA16EC23FA598712" box="[116,1471,1077,1092]" gridrow="6" pageId="2" pageNumber="3">
|
||
<th id="57450F79FFAF007DEA16EC23FEA48712" box="[116,322,1077,1092]" gridcol="0" gridrow="6" pageId="2" pageNumber="3">Pattypan squash</th>
|
||
<td id="57450F79FFAF007DEBE8EC23FE278712" box="[394,449,1077,1092]" gridcol="1" gridrow="6" pageId="2" pageNumber="3">1.0</td>
|
||
<td id="57450F79FFAF007DE86BEC23FDBF8712" box="[521,601,1077,1092]" gridcol="2" gridrow="6" pageId="2" pageNumber="3">1.6</td>
|
||
<td id="57450F79FFAF007DE8C3EC23FD098712" box="[673,751,1077,1092]" gridcol="3" gridrow="6" pageId="2" pageNumber="3">5.6</td>
|
||
<td id="57450F79FFAF007DE955EC23FC618712" box="[823,903,1077,1092]" gridcol="4" gridrow="6" pageId="2" pageNumber="3">9.4</td>
|
||
<td id="57450F79FFAF007DE9ADEC23FBE58712" box="[975,1027,1077,1092]" gridcol="5" gridrow="6" pageId="2" pageNumber="3">6.9</td>
|
||
<td id="57450F79FFAF007DEE29EC23FB7E8712" box="[1099,1176,1077,1092]" gridcol="6" gridrow="6" pageId="2" pageNumber="3">7.1</td>
|
||
<td id="57450F79FFAF007DEEBDEC23FACC8712" box="[1247,1322,1077,1092]" gridcol="7" gridrow="6" pageId="2" pageNumber="3">25.3</td>
|
||
<td id="57450F79FFAF007DEF10EC23FA598712" box="[1394,1471,1077,1092]" gridcol="8" gridrow="6" pageId="2" pageNumber="3">43.1</td>
|
||
</tr>
|
||
<tr id="14946605FFAF007DEA16EC5EFA59870C" box="[116,1471,1096,1114]" gridrow="7" pageId="2" pageNumber="3">
|
||
<th id="57450F79FFAF007DEA16EC5EFEA4870C" box="[116,322,1096,1114]" gridcol="0" gridrow="7" pageId="2" pageNumber="3">
|
||
<taxonomicName id="6DA41FC4FFAFFF80EA16EC5AFF1D870C" box="[116,251,1100,1114]" class="Magnoliopsida" family="Cucurbitaceae" genus="Echinocystis" kingdom="Plantae" order="Cucurbitales" pageId="2" pageNumber="3" phylum="Tracheophyta" rank="species" species="lobata">
|
||
<emphasis id="98D0B855FFAFFF80EA16EC5AFF1D870C" bold="true" box="[116,251,1100,1114]" italics="true" pageId="2" pageNumber="3">Echinocystis lobata</emphasis>
|
||
</taxonomicName>
|
||
a
|
||
</th>
|
||
<td id="57450F79FFAF007DEBE8EC5EFE27870C" box="[394,449,1096,1114]" gridcol="1" gridrow="7" pageId="2" pageNumber="3">8.7</td>
|
||
<td id="57450F79FFAF007DE86BEC5EFDBF870C" box="[521,601,1096,1114]" gridcol="2" gridrow="7" pageId="2" pageNumber="3">2.2</td>
|
||
<td id="57450F79FFAF007DE8C3EC5EFD09870C" box="[673,751,1096,1114]" gridcol="3" gridrow="7" pageId="2" pageNumber="3">15.2</td>
|
||
<td id="57450F79FFAF007DE955EC5EFC61870C" box="[823,903,1096,1114]" gridcol="4" gridrow="7" pageId="2" pageNumber="3">64.4</td>
|
||
<td id="57450F79FFAF007DE9ADEC5EFBE5870C" box="[975,1027,1096,1114]" gridcol="5" gridrow="7" pageId="2" pageNumber="3">3.4</td>
|
||
<td id="57450F79FFAF007DEE29EC5EFB7E870C" box="[1099,1176,1096,1114]" gridcol="6" gridrow="7" pageId="2" pageNumber="3">0.4</td>
|
||
<td id="57450F79FFAF007DEEBDEC5EFACC870C" box="[1247,1322,1096,1114]" gridcol="7" gridrow="7" pageId="2" pageNumber="3">1.8</td>
|
||
<td id="57450F79FFAF007DEF10EC5EFA59870C" box="[1394,1471,1096,1114]" gridcol="8" gridrow="7" pageId="2" pageNumber="3">3.8</td>
|
||
</tr>
|
||
<tr id="14946605FFAF007DEA16EC49FA598727" box="[116,1471,1119,1137]" gridrow="8" pageId="2" pageNumber="3">
|
||
<th id="57450F79FFAF007DEA16EC49FEA48727" box="[116,322,1119,1137]" gridcol="0" gridrow="8" pageId="2" pageNumber="3">
|
||
<taxonomicName id="6DA41FC4FFAFFF80EA16EC75FF038727" baseAuthorityName="Sundquist and Rydberg" baseAuthorityYear="1983" box="[116,229,1123,1137]" class="Magnoliopsida" family="Cucurbitaceae" genus="Luffa" kingdom="Plantae" order="Cucurbitales" pageId="2" pageNumber="3" phylum="Tracheophyta" rank="species" species="cylindrica">
|
||
<emphasis id="98D0B855FFAFFF80EA16EC75FF038727" bold="true" box="[116,229,1123,1137]" italics="true" pageId="2" pageNumber="3">Luffa cylindrica</emphasis>
|
||
</taxonomicName>
|
||
a
|
||
</th>
|
||
<td id="57450F79FFAF007DEBE8EC49FE278727" box="[394,449,1119,1137]" gridcol="1" gridrow="8" pageId="2" pageNumber="3">23.6</td>
|
||
<td id="57450F79FFAF007DE86BEC49FDBF8727" box="[521,601,1119,1137]" gridcol="2" gridrow="8" pageId="2" pageNumber="3">3.7</td>
|
||
<td id="57450F79FFAF007DE8C3EC49FD098727" box="[673,751,1119,1137]" gridcol="3" gridrow="8" pageId="2" pageNumber="3">4.2</td>
|
||
<td id="57450F79FFAF007DE955EC49FC618727" box="[823,903,1119,1137]" gridcol="4" gridrow="8" pageId="2" pageNumber="3">68.6</td>
|
||
<td id="57450F79FFAF007DE9ADEC49FBE58727" box="[975,1027,1119,1137]" gridcol="5" gridrow="8" pageId="2" pageNumber="3">0</td>
|
||
<td id="57450F79FFAF007DEE29EC49FB7E8727" box="[1099,1176,1119,1137]" gridcol="6" gridrow="8" pageId="2" pageNumber="3">0</td>
|
||
<td id="57450F79FFAF007DEEBDEC49FACC8727" box="[1247,1322,1119,1137]" gridcol="7" gridrow="8" pageId="2" pageNumber="3">0</td>
|
||
<td id="57450F79FFAF007DEF10EC49FA598727" box="[1394,1471,1119,1137]" gridcol="8" gridrow="8" pageId="2" pageNumber="3">0</td>
|
||
</tr>
|
||
<tr id="14946605FFAF007DEA16EC6CFA5987DE" box="[116,1471,1146,1160]" gridrow="9" pageId="2" pageNumber="3">
|
||
<th id="57450F79FFAF007DEA16EC6CFEA487DE" box="[116,322,1146,1160]" gridcol="0" gridrow="9" pageId="2" pageNumber="3">
|
||
<taxonomicName id="6DA41FC4FFAFFF80EA16EC6CFED587DE" box="[116,307,1146,1160]" class="Magnoliopsida" family="Cucurbitaceae" genus="Cyclanthera" kingdom="Plantae" order="Cucurbitales" pageId="2" pageNumber="3" phylum="Tracheophyta" rank="species" species="brachystachys">
|
||
<emphasis id="98D0B855FFAFFF80EA16EC6CFED587DE" bold="true" box="[116,307,1146,1160]" italics="true" pageId="2" pageNumber="3">Cyclanthera brachystachys</emphasis>
|
||
</taxonomicName>
|
||
</th>
|
||
<td id="57450F79FFAF007DEBE8EC6CFE2787DE" box="[394,449,1146,1160]" gridcol="1" gridrow="9" pageId="2" pageNumber="3">0.2</td>
|
||
<td id="57450F79FFAF007DE86BEC6CFDBF87DE" box="[521,601,1146,1160]" gridcol="2" gridrow="9" pageId="2" pageNumber="3">0</td>
|
||
<td id="57450F79FFAF007DE8C3EC6CFD0987DE" box="[673,751,1146,1160]" gridcol="3" gridrow="9" pageId="2" pageNumber="3">1.1</td>
|
||
<td id="57450F79FFAF007DE955EC6CFC6187DE" box="[823,903,1146,1160]" gridcol="4" gridrow="9" pageId="2" pageNumber="3">89.3</td>
|
||
<td id="57450F79FFAF007DE9ADEC6CFBE587DE" box="[975,1027,1146,1160]" gridcol="5" gridrow="9" pageId="2" pageNumber="3">0.1</td>
|
||
<td id="57450F79FFAF007DEE29EC6CFB7E87DE" box="[1099,1176,1146,1160]" gridcol="6" gridrow="9" pageId="2" pageNumber="3">0</td>
|
||
<td id="57450F79FFAF007DEEBDEC6CFACC87DE" box="[1247,1322,1146,1160]" gridcol="7" gridrow="9" pageId="2" pageNumber="3">0.7</td>
|
||
<td id="57450F79FFAF007DEF10EC6CFA5987DE" box="[1394,1471,1146,1160]" gridcol="8" gridrow="9" pageId="2" pageNumber="3">8.6</td>
|
||
</tr>
|
||
</table>
|
||
</paragraph>
|
||
<paragraph id="AA1B6447FFAFFF80EA11EC8FFF0D87F9" blockId="2.[115,235,1177,1199]" box="[115,235,1177,1199]" pageId="2" pageNumber="3">
|
||
<tableNote id="574265C9FFAFFF80EA11EC8FFF0D87F9" box="[115,235,1177,1199]" pageId="2" pageNumber="3" targetBox="[116,1471,905,1160]" targetPageId="2">
|
||
<superScript id="5DD1C90FFFAFFF80EA11EC8FFF9C87F3" attach="left" box="[115,122,1177,1189]" fontSize="5" pageId="2" pageNumber="3">a</superScript>
|
||
Fresh seeds.
|
||
</tableNote>
|
||
</paragraph>
|
||
<paragraph id="AA1B6447FFAFFF80EAE6EED8FECC84DE" blockId="2.[100,770,1240,1956]" pageId="2" pageNumber="3">
|
||
When an analogous experiment was performed with a different species,
|
||
<taxonomicName id="6DA41FC4FFAFFF80EAD3EEFCFEF785AB" box="[177,273,1770,1789]" class="Magnoliopsida" family="Cucurbitaceae" genus="Cucurbita" kingdom="Plantae" order="Cucurbitales" pageId="2" pageNumber="3" phylum="Tracheophyta" rank="species" species="maxima">
|
||
<emphasis id="98D0B855FFAFFF80EAD3EEFCFEF785AB" bold="true" box="[177,273,1770,1789]" italics="true" pageId="2" pageNumber="3">C. maxima</emphasis>
|
||
</taxonomicName>
|
||
‘Bambino’, the pigment content changes were similar to those for
|
||
<taxonomicName id="6DA41FC4FFAFFF80EAB8EF13FEF8844F" baseAuthorityName="Ellsworth and Nowak" baseAuthorityYear="1974" box="[218,286,1797,1817]" class="Magnoliopsida" family="Cucurbitaceae" genus="Cucurbita" kingdom="Plantae" order="Cucurbitales" pageId="2" pageNumber="3" phylum="Tracheophyta" rank="species" species="pepo">
|
||
<emphasis id="98D0B855FFAFFF80EAB8EF13FEF8844F" bold="true" box="[218,286,1797,1817]" italics="true" pageId="2" pageNumber="3">C. pepo</emphasis>
|
||
</taxonomicName>
|
||
‘Miranda’ in the control plants, i.e., Chl
|
||
<emphasis id="98D0B855FFAFFF80E8CBEF10FD52844F" bold="true" box="[681,692,1798,1817]" italics="true" pageId="2" pageNumber="3">a</emphasis>
|
||
content increased gradually, while that of Chl-GG and Pchl-GG did not change significantly during illumination (
|
||
<figureCitation id="329F78C2FFAFFF80EBD0EF2BFE0A8406" box="[434,492,1853,1872]" captionStart="Fig" captionStartId="6.[100,130,691,708]" captionTargetBox="[110,763,150,662]" captionTargetId="figure-540@6.[106,767,148,664]" captionTargetPageId="6" captionText="Fig. 6. The content of Chl a, Pchl-GG and Chl-GG in the primary leaves of 11- day-old etiolated Cucurbita maxima ‘Bambino’ seedlings during illumination. The data are means ± SE, n = 3–4. The pigments were extracted and analyzed using HPLC as described in the Experimental section." figureDoi="http://doi.org/10.5281/zenodo.8235044" httpUri="https://zenodo.org/record/8235044/files/figure.png" pageId="2" pageNumber="3">Fig. 6</figureCitation>
|
||
). The content of individual Pchls was dominated by Pchl-GG and did not change very much during illumination (
|
||
<figureCitation id="329F78C2FFAFFF80EA85EF63FEFA84DE" box="[231,284,1909,1928]" captionStart="Fig" captionStartId="5.[818,848,1774,1791]" captionTargetBox="[826,1484,148,1747]" captionTargetId="figure-96@5.[823,1484,148,1747]" captionText="Fig. 5. Composition of protochlorophylls in primary leaves of 11-day-old etiolated Cucurbita pepo ‘Miranda’ and C. maxima ‘Bambino’ seedlings during illumination. In the case of ALA-treated plants, the 11-day-old seedlings were incubated for additional 3 days in 10 mM ALA solution in the dark before illumination. The data are means ± SE, n = 3–4. The pigments were extracted and analyzed using HPLC as described in the Experimental section." figureDoi="http://doi.org/10.5281/zenodo.8257061" httpUri="https://zenodo.org/record/8257061/files/figure.png" pageId="2" pageNumber="3">Fig. 5</figureCitation>
|
||
).
|
||
</paragraph>
|
||
<paragraph id="AA1B6447FFAFFF80EAE6EF87FBC685AB" blockId="2.[100,770,1240,1956]" lastBlockId="2.[818,1488,1240,1789]" pageId="2" pageNumber="3">
|
||
Next, we tried to follow Pchl synthase activity using different preparations, namely homogenates of seed coats, the inner seed coats of
|
||
<taxonomicName id="6DA41FC4FFAFFF80E950ECE2FC748651" box="[818,914,1268,1287]" class="Magnoliopsida" family="Cucurbitaceae" genus="Cucurbita" kingdom="Plantae" order="Cucurbitales" pageId="2" pageNumber="3" phylum="Tracheophyta" rank="species" species="maxima">
|
||
<emphasis id="98D0B855FFAFFF80E950ECE2FC748651" bold="true" box="[818,914,1268,1287]" italics="true" pageId="2" pageNumber="3">C. maxima</emphasis>
|
||
</taxonomicName>
|
||
‘Bambino’, prolammelar bodies (PLB) isolated from etiolated leaves of
|
||
<taxonomicName id="6DA41FC4FFAFFF80E9EEED19FC088674" box="[908,1006,1295,1314]" class="Magnoliopsida" family="Cucurbitaceae" genus="Cucurbita" kingdom="Plantae" order="Cucurbitales" pageId="2" pageNumber="3" phylum="Tracheophyta" rank="species" species="maxima">
|
||
<emphasis id="98D0B855FFAFFF80E9EEED19FC088674" bold="true" box="[908,1006,1295,1314]" italics="true" pageId="2" pageNumber="3">C. maxima</emphasis>
|
||
</taxonomicName>
|
||
‘Bambino’ and homogenates of etiolated leaves of
|
||
<taxonomicName id="6DA41FC4FFAFFF80E950ED3DFC958669" baseAuthorityName="Ellsworth and Nowak" baseAuthorityYear="1974" box="[818,883,1323,1343]" class="Magnoliopsida" family="Cucurbitaceae" genus="Cucurbita" kingdom="Plantae" order="Cucurbitales" pageId="2" pageNumber="3" phylum="Tracheophyta" rank="species" species="pepo">
|
||
<emphasis id="98D0B855FFAFFF80E950ED3DFC958669" bold="true" box="[818,883,1323,1343]" italics="true" pageId="2" pageNumber="3">C. pepo</emphasis>
|
||
</taxonomicName>
|
||
‘Miranda’. Different isolation buffers and additional preparation treatments, such as repeated freeze-thawing, were also used. We applied a broad substrate concentration range of both Pchlide and side-chain precursors (GG-PP and phytol-PP) and incubation periods (see Experimental section). However, in none of the preparations was Pchl synthase activity found. This suggests the latency of the synthase and/or that the reaction rate of the enzyme is slow, like what was demonstrated in the case of Chl synthase (
|
||
<bibRefCitation id="CE3519B6FFAFFF80EE67EDF9FB2D8554" author="Lindsten, A. & Welch, C. J. & Schoch, S. & Ryberg, M. & Rudiger, W. & Sundqvist, C." box="[1029,1227,1519,1538]" pageId="2" pageNumber="3" pagination="277 - 285" refId="ref7404" refString="Lindsten, A., Welch, C. J., Schoch, S., Ryberg, M., Rudiger, W., Sundqvist, C., 1990. Chlorophyll synthetase is latent in well preserved prolamellar bodies of etiolated wheat. Physiol. Plantarum 80, 277 - 285. https: // doi. org / 10.1111 / j. 1399 - 3054.1990. tb 04408. x." type="journal article" year="1990">Lindsten et al., 1990</bibRefCitation>
|
||
). It is also possible that in seeds the enzyme is active only at the early stages of seed development which were not analyzed in our case. Nevertheless, the homogenates of etiolated leaves of
|
||
<taxonomicName id="6DA41FC4FFAFFF80E99BEE54FBA58500" baseAuthorityName="Ellsworth and Nowak" baseAuthorityYear="1974" box="[1017,1091,1602,1622]" class="Magnoliopsida" family="Cucurbitaceae" genus="Cucurbita" kingdom="Plantae" order="Cucurbitales" pageId="2" pageNumber="3" phylum="Tracheophyta" rank="species" species="pepo">
|
||
<emphasis id="98D0B855FFAFFF80E99BEE54FBA58500" bold="true" box="[1017,1091,1602,1622]" italics="true" pageId="2" pageNumber="3">C. pepo</emphasis>
|
||
</taxonomicName>
|
||
‘Miranda’ were active in Chl-GG and Chl-phytol synthesis, using Chlide and appropriate side-chain substrates (
|
||
<figureCitation id="329F78C2FFAFFF80E958EE6CFC9D85DB" box="[826,891,1658,1677]" captionStart="Fig" captionStartId="3.[100,130,1741,1758]" captionTargetBox="[109,764,1207,1712]" captionTargetId="figure-535@3.[106,767,1205,1713]" captionTargetPageId="3" captionText="Fig. 3. HPLC chromatogram of porphyrins extracted from the seed coat of pumpkin (Cucurbita pepo) seeds using absorption detection at 420 nm. The analysis was performed using a C18 Teknokroma column, methanol/acetonitrile (1:1) as eluent and 1.5 ml/min flow." figureDoi="http://doi.org/10.5281/zenodo.8235040" httpUri="https://zenodo.org/record/8235040/files/figure.png" pageId="2" pageNumber="3">Fig. S3</figureCitation>
|
||
), indicating that the assay method, at least for the chlorophyll synthase activity, is appropriate. Both Chl-GG and Chl-phytol were found as double peaks, showing identical absorption maxima to those of Chl
|
||
<emphasis id="98D0B855FFAFFF80E934EED8FC8785B7" bold="true" box="[854,865,1742,1761]" italics="true" pageId="2" pageNumber="3">a</emphasis>
|
||
, and result probably from the presence of both cis/trans isomers of the side-chain substrates.
|
||
</paragraph>
|
||
</subSubSection>
|
||
</treatment>
|
||
</document> |