146 lines
16 KiB
XML
146 lines
16 KiB
XML
<document id="F49A42E81A669EDF5D3FA0D4A7B53FBC" ID-CLB-Dataset="285686" ID-DOI="10.1016/j.phytochem.2016.02.011" ID-GBIF-Dataset="5613c391-71cd-4dac-b03b-1f026c15b6e2" ID-ISSN="1873-3700" ID-Zenodo-Dep="10530233" IM.bibliography_approvedBy="julia" IM.illustrations_approvedBy="julia" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.taxonomicNames_approvedBy="julia" IM.treatments_approvedBy="julia" checkinTime="1704942941654" checkinUser="felipe" docAuthor="Ricigliano, Vincent, Kumar, Santosh, Kinison, Scott, Brooks, Christopher, Nybo, S. Eric, Chappell, Joe & Howarth, Dianella G." docDate="2016" docId="039ADC33D902FF8E7F218915FC1CFDA8" docLanguage="en" docName="Phytochemistry.125.43-53.pdf" docOrigin="Phytochemistry 125" docSource="http://dx.doi.org/10.1016/j.phytochem.2016.02.011" docStyle="DocumentStyle:9E596C34F4E94307D29315B03ACE1007.6:Phytochemistry.2014-2019.journal_article" docStyleId="9E596C34F4E94307D29315B03ACE1007" docStyleName="Phytochemistry.2014-2019.journal_article" docStyleVersion="6" docTitle="Valeriana officinalis subsp. hairy" docType="treatment" docVersion="3" lastPageNumber="45" masterDocId="FFA3A44BD900FF8C7F538F51FFC1FF9C" masterDocTitle="Regulation of sesquiterpenoid metabolism in recombinant and elicited Valeriana officinalis hairy roots" masterLastPageNumber="53" masterPageNumber="43" pageNumber="45" updateTime="1705609558154" updateUser="ExternalLinkService">
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<mods:title id="D27419B64ECB3C9AE645AE499F343E33">Regulation of sesquiterpenoid metabolism in recombinant and elicited Valeriana officinalis hairy roots</mods:title>
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<mods:namePart id="5390A6E0E11211629C0795F082AA7413">Ricigliano, Vincent</mods:namePart>
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<mods:affiliation id="7D2EA05E5FFA9400EB22DD9E648F26D3">Department of Biological Sciences, St. John’s University, Jamaica, NY 11439, United States</mods:affiliation>
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<mods:namePart id="71D62FCD52C171A158A329297598298D">Kumar, Santosh</mods:namePart>
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<mods:namePart id="3B8FCE5320093F2D857523FBCA7D2CF6">Kinison, Scott</mods:namePart>
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<mods:namePart id="8552E3E2D97E0D0B47E99FD57EADA2B8">Brooks, Christopher</mods:namePart>
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<mods:namePart id="DA712E6AB3EB8A1D6173AB82E5B2F8BC">Nybo, S. Eric</mods:namePart>
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<mods:namePart id="432E9801F3147EF6125961A613324DFE">Chappell, Joe</mods:namePart>
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<mods:namePart id="924D6A0B6E9C6E1B6E65E4818BEC9C68">Howarth, Dianella G.</mods:namePart>
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<mods:title id="E3A25D6A2FE6D932F29A06BE9FC0D7AD">Phytochemistry</mods:title>
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<mods:date id="28D380BF048D52552A6A701581695EC3">2016</mods:date>
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<treatment id="039ADC33D902FF8E7F218915FC1CFDA8" ID-DOI="http://doi.org/10.5281/zenodo.10530239" ID-Zenodo-Dep="10530239" LSID="urn:lsid:plazi:treatment:039ADC33D902FF8E7F218915FC1CFDA8" httpUri="http://treatment.plazi.org/id/039ADC33D902FF8E7F218915FC1CFDA8" lastPageNumber="45" pageId="2" pageNumber="45">
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<subSubSection id="C3293EAED902FF8E7F218915FD35F9C4" box="[114,756,1604,1624]" pageId="2" pageNumber="45" type="nomenclature">
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<paragraph id="8B8C6D25D902FF8E7F218915FD35F9C4" blockId="2.[114,756,1604,1624]" box="[114,756,1604,1624]" pageId="2" pageNumber="45">
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<heading id="D0C4DA49D902FF8E7F218915FD35F9C4" box="[114,756,1604,1624]" fontSize="36" level="2" pageId="2" pageNumber="45" reason="3">
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<emphasis id="B947B137D902FF8E7F218915FD35F9C4" box="[114,756,1604,1624]" italics="true" pageId="2" pageNumber="45">
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2.1. Establishment and characterization of
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<taxonomicName id="4C3316A6D902FF8E7D5C8915FD45F9C4" authority="L." box="[527,644,1604,1624]" class="Magnoliopsida" family="Caprifoliaceae" genus="Valeriana" kingdom="Plantae" order="Dipsacales" pageId="2" pageNumber="45" phylum="Tracheophyta" rank="subSpecies" species="officinalis" subSpecies="hairy">V. officinalis</taxonomicName>
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hairy roots
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</emphasis>
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</paragraph>
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<subSubSection id="C3293EAED902FF8E7FC2892DFC1CFDA8" pageId="2" pageNumber="45" type="description">
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<paragraph id="8B8C6D25D902FF8E7FC2892DFE47F83B" blockId="2.[113,784,1660,2015]" pageId="2" pageNumber="45">
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<taxonomicName id="4C3316A6D902FF8E7FC2892DFECBF90C" box="[145,266,1660,1680]" class="Magnoliopsida" family="Caprifoliaceae" genus="Valeriana" kingdom="Plantae" order="Dipsacales" pageId="2" pageNumber="50" phylum="Tracheophyta" rank="subSpecies" species="officinalis" subSpecies="hairy">
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<emphasis id="B947B137D902FF8E7FC2892DFECBF90C" box="[145,266,1660,1680]" italics="true" pageId="2" pageNumber="45">V. officinalis</emphasis>
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</taxonomicName>
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hairy roots were generated using
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<taxonomicName id="4C3316A6D902FF8E7DD2892DFF16F930" authorityName="Conn" authorityYear="1942" baseAuthorityName="Riker et al." baseAuthorityYear="1930" class="Alphaproteobacteria" family="Rhizobiaceae" genus="Agrobacterium" kingdom="Bacteria" order="Rhizobiales" pageId="2" pageNumber="45" phylum="Proteobacteria" rank="species" species="rhizogenes">
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<emphasis id="B947B137D902FF8E7DD2892DFF16F930" italics="true" pageId="2" pageNumber="45">Agrobacterium rhizogenes</emphasis>
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</taxonomicName>
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-mediated transformation of
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<emphasis id="B947B137D902FF8E7EA989C9FDFEF930" box="[506,575,1688,1708]" italics="true" pageId="2" pageNumber="45">in vitro</emphasis>
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grown leaf explants. This genetic manipulation relies on conjugative transfer and integration of
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<taxonomicName id="4C3316A6D902FF8E7F8D899EFE9EF97F" authorityName="Conn" authorityYear="1942" baseAuthorityName="Riker et al." baseAuthorityYear="1930" box="[222,351,1743,1763]" class="Alphaproteobacteria" family="Rhizobiaceae" genus="Agrobacterium" kingdom="Bacteria" order="Rhizobiales" pageId="2" pageNumber="45" phylum="Proteobacteria" rank="species" species="rhizogenes">
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<emphasis id="B947B137D902FF8E7F8D899EFE9EF97F" box="[222,351,1743,1763]" italics="true" pageId="2" pageNumber="45">A. rhizogenes</emphasis>
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</taxonomicName>
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root-inducing plasmid DNA into the host plant nuclear genome.
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<taxonomicName id="4C3316A6D902FF8E7E0C89BAFE17F963" box="[351,470,1771,1791]" class="Magnoliopsida" family="Caprifoliaceae" genus="Valeriana" kingdom="Plantae" order="Dipsacales" pageId="2" pageNumber="45" phylum="Tracheophyta" rank="species" species="officinalis">
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<emphasis id="B947B137D902FF8E7E0C89BAFE17F963" box="[351,470,1771,1791]" italics="true" pageId="2" pageNumber="45">V. officinalis</emphasis>
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</taxonomicName>
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seeds were aseptically germinated and hairy roots were initiated on
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<emphasis id="B947B137D902FF8E7EAE8856FD83F887" box="[509,578,1799,1819]" italics="true" pageId="2" pageNumber="45">in vitro</emphasis>
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leaf explants. Single root segments were removed from different explants to afford independent transgenic events, which were maintained on hormone-free solid medium. Isogenic lines were used to initiate shake flask cultures and generate clonal biomass for molecular and biochemical analyses (
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<figureCitation id="130871A0D902FF8E7E6488C5FEB8F83B" box="[311,377,1940,1959]" captionStart="Fig" captionStartId="1.[275,301,1548,1562]" captionTargetBox="[337,1218,181,1519]" captionTargetId="figure-307@1.[337,1218,181,1519]" captionTargetPageId="1" captionText="Fig. 1. An outline for hypothetical sesquiterpenoid biosynthetic pathways operating in V. officinalis. Not all steps are shown." figureDoi="http://doi.org/10.5281/zenodo.10530235" httpUri="https://zenodo.org/record/10530235/files/figure.png" pageId="2" pageNumber="45">Fig. S1</figureCitation>
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).
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</paragraph>
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<paragraph id="8B8C6D25D902FF8E7FC288E1FC1CFDA8" blockId="2.[113,784,1660,2015]" lastBlockId="2.[831,1501,182,564]" pageId="2" pageNumber="45">
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As a metabolic qualification of the hairy root cultures, chemical profiles of hairy roots transformed with
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<taxonomicName id="4C3316A6D902FF8E7D5F889BFD4AF842" authorityName="Conn" authorityYear="1942" baseAuthorityName="Riker et al." baseAuthorityYear="1930" box="[524,651,1994,2014]" class="Alphaproteobacteria" family="Rhizobiaceae" genus="Agrobacterium" kingdom="Bacteria" order="Rhizobiales" pageId="2" pageNumber="45" phylum="Proteobacteria" rank="species" species="rhizogenes">
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<emphasis id="B947B137D902FF8E7D5F889BFD4AF842" box="[524,651,1994,2014]" italics="true" pageId="2" pageNumber="45">A. rhizogenes</emphasis>
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</taxonomicName>
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harboring Ri plasmid pRi15834 were compared to those of roots from wild
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<typeStatus id="5488D387D902FF8E7AF88FE6FA18FF56" box="[1451,1497,183,202]" pageId="2" pageNumber="45">type</typeStatus>
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, soil-grown
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<taxonomicName id="4C3316A6D902FF8E7CE18F80FBE6FF79" box="[946,1063,209,229]" class="Magnoliopsida" family="Caprifoliaceae" genus="Valeriana" kingdom="Plantae" order="Dipsacales" pageId="2" pageNumber="45" phylum="Tracheophyta" rank="species" species="officinalis">
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<emphasis id="B947B137D902FF8E7CE18F80FBE6FF79" box="[946,1063,209,229]" italics="true" pageId="2" pageNumber="45">V. officinalis</emphasis>
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</taxonomicName>
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plants. Root biomass was powdered in liquid nitrogen, the materials fully lysed by mixing with an equal volume of acetone, followed by the addition of an equal volume of water, then partitioned into hexane. Aliquots of the hexane extracts were analyzed directly by GC–MS, as well as dried under nitrogen prior to trimethylsilyl diazomethane derivatization for GC–MS (
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<figureCitation id="130871A0D902FF8E7CC58E28FC0CFE11" box="[918,973,377,397]" captionStart="Fig" captionStartId="3.[87,113,829,843]" captionTargetBox="[329,1236,181,800]" captionTargetId="figure-302@3.[329,1236,181,800]" captionTargetPageId="3" captionText="Fig. 2. GC–MS profiles comparison of sesquiterpenoid chemistry within V. officinalis hairy root to wild type, soil grown plant root. Extracts prepared from intact, wild type plant root (panels C, D) are compared to a hairy root line harboring the A. rhizogenes pRi15834 T-DNA insertion (panels A, B). The extracts were examined directly (panels A, C) or after derivatization (panels B, D) by GC analysis. The peaks for β-caryophyllene (a), valerenadiene (b), valerenal (c) and valerenic acid (d) are annotated to facilitate comparisons." figureDoi="http://doi.org/10.5281/zenodo.10530237" httpUri="https://zenodo.org/record/10530237/files/figure.png" pageId="2" pageNumber="45">Fig. 2</figureCitation>
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,
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<figureCitation id="130871A0D902FF8E7C8B8E28FBDBFE11" box="[984,1050,377,397]" captionStart="Fig" captionStartId="6.[113,139,884,898]" captionTargetBox="[272,1332,183,854]" captionTargetId="figure-209@6.[272,1332,181,854]" captionTargetPageId="6" captionText="Fig. 7. Establishment of binary vector co-transformation conditions and heterologous gene expression. (a) Kanamycin sensitivity assay of pRi15834 control hairy roots. Root tips were inoculated to MS medium containing a range of kanamycin concentrations (0, 20, 40, 60, 80 mg/L) and qualified after 30 days. (b) GUS histochemical staining of cotransformed hairy roots. The lines harbored the pVo257 T-DNA, which contained a GUS-intron overexpression cassette, or the empty vector control (pVo257 EV). The three independent GUS lines exhibited varying degrees of staining, whereas no staining occurred in the empty vector line (C) Magnified visualization of GUS expression along the root axis including individual root hairs." figureDoi="http://doi.org/10.5281/zenodo.10530255" httpUri="https://zenodo.org/record/10530255/files/figure.png" pageId="2" pageNumber="45">Fig. S7</figureCitation>
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). The chemical profile of extracts from roots of wild
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<typeStatus id="5488D387D902FF8E7CC28EC7FC7FFE35" box="[913,958,406,425]" pageId="2" pageNumber="45">type</typeStatus>
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plants exhibited qualitative and quantitative differences relative to the hairy root cultures. The pRi15834 lines tended to accumulate fewer constituents than the roots from soil grown plants, but the levels of β- caryophyllene (
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<emphasis id="B947B137D902FF8E7B8C8EB8FB2DFE60" bold="true" box="[1247,1260,489,508]" pageId="2" pageNumber="45">5</emphasis>
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) and valerenal (
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<emphasis id="B947B137D902FF8E7AC38EB8FA5CFE60" bold="true" box="[1424,1437,489,508]" pageId="2" pageNumber="45">7</emphasis>
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) were elevated while valerenadiene (
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<emphasis id="B947B137D902FF8E7B278D54FB40FD84" bold="true" box="[1140,1153,517,536]" pageId="2" pageNumber="45">6</emphasis>
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) and valerenic acid (
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<emphasis id="B947B137D902FF8E7A068D54FAA3FD84" bold="true" box="[1365,1378,517,536]" pageId="2" pageNumber="45">8</emphasis>
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) were comparable (
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<figureCitation id="130871A0D902FF8E7CCA8D70FC11FDA8" box="[921,976,545,564]" captionStart="Fig" captionStartId="3.[87,113,829,843]" captionTargetBox="[329,1236,181,800]" captionTargetId="figure-302@3.[329,1236,181,800]" captionTargetPageId="3" captionText="Fig. 2. GC–MS profiles comparison of sesquiterpenoid chemistry within V. officinalis hairy root to wild type, soil grown plant root. Extracts prepared from intact, wild type plant root (panels C, D) are compared to a hairy root line harboring the A. rhizogenes pRi15834 T-DNA insertion (panels A, B). The extracts were examined directly (panels A, C) or after derivatization (panels B, D) by GC analysis. The peaks for β-caryophyllene (a), valerenadiene (b), valerenal (c) and valerenic acid (d) are annotated to facilitate comparisons." figureDoi="http://doi.org/10.5281/zenodo.10530237" httpUri="https://zenodo.org/record/10530237/files/figure.png" pageId="2" pageNumber="45">Fig. 2</figureCitation>
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).
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</paragraph>
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</subSubSection>
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</treatment>
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