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<document ID-DOI="http://dx.doi.org/10.3897/BDJ.9.e59648" ID-PMC="PMC7835198" ID-Pensoft-Pub="1314-2828-9-e59648" ID-Pensoft-UUID="2E56CD86B4425E9A8825FFF492BF19C8" ID-PubMed="33519261" ModsDocID="1314-2828-9-e59648" checkinTime="1611008215846" checkinUser="pensoft" docAuthor="Wijesinghe, Subodini N., Wang, Yong, Zucconi, Laura, Dayarathne, Monika C., Boonmee, Saranyaphat, Camporesi, Erio, Wanasinghe, Dhanushka N. &amp; Hyde, Kevin D." docDate="2021" docId="29E4C2F05ADA5F9389E0A48495626C05" docLanguage="en" docName="BiodivDatJour 9: e59648" docOrigin="Biodiversity Data Journal 9" docPubDate="2021-01-18" docSource="http://dx.doi.org/10.3897/BDJ.9.e59648" docTitle="Pseudoophiobolus mathieui Phookamsak., Wanas., S. K. Huang, Camporesi &amp; K. D. Hyde 2017" docType="treatment" docVersion="2" id="2E56CD86B4425E9A8825FFF492BF19C8" lastPageNumber="59648" masterDocId="2E56CD86B4425E9A8825FFF492BF19C8" masterDocTitle="Additions to Italian Pleosporinae, including Italica heraclei sp. nov." masterLastPageNumber="59648" masterPageNumber="59648" pageNumber="59648" updateTime="1668128608865" updateUser="ExternalLinkService">
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<mods:titleInfo>
<mods:title>Additions to Italian Pleosporinae, including Italica heraclei sp. nov.</mods:title>
</mods:titleInfo>
<mods:name type="personal">
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<mods:roleTerm>Author</mods:roleTerm>
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<mods:namePart>Wijesinghe, Subodini N.</mods:namePart>
<mods:nameIdentifier type="ORCID">https://orcid.org/0000-0002-5625-9578</mods:nameIdentifier>
<mods:affiliation>Department of Plant Pathology, Agriculture College, Guizhou University, Guiyang, Guizhou Province, 550025, China &amp; Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand &amp; School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand</mods:affiliation>
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<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
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<mods:namePart>Wang, Yong</mods:namePart>
<mods:affiliation>Department of Plant Pathology, Agriculture College, Guizhou University, Guiyang, Guizhou Province, 550025, China</mods:affiliation>
<mods:nameIdentifier type="email">yongwangbis@aliyun.com</mods:nameIdentifier>
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<mods:roleTerm>Author</mods:roleTerm>
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<mods:namePart>Zucconi, Laura</mods:namePart>
<mods:affiliation>Department of Ecological and Biological Sciences, University of Tuscia, Largo dell'Universita snc, 01100, Viterbo, Italy</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Dayarathne, Monika C.</mods:namePart>
<mods:affiliation>Department of Plant Pathology, Agriculture College, Guizhou University, Guiyang, Guizhou Province, 550025, China</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Boonmee, Saranyaphat</mods:namePart>
<mods:affiliation>Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand &amp; School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Camporesi, Erio</mods:namePart>
<mods:affiliation>A. M. B. Gruppo Micologico Forlivese &quot; Antonio Cicognani &quot;, Via Roma 18, Forli, Italy</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Wanasinghe, Dhanushka N.</mods:namePart>
<mods:nameIdentifier type="ORCID">https://orcid.org/0000-0003-1759-3933</mods:nameIdentifier>
<mods:affiliation>CAS Key Laboratory for Plant Biodiversity and Biogeography of East Asia (KLPB), Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan, China &amp; East and Central Asia Regional Office, World Agroforestry Centre (ICRAF), Kunming 650201, Yunnan, China &amp; Honghe Center for Mountain Futures, Kunming Institute of Botany, Chinese Academy of Sciences, Honghe County, Yunnan, China</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Hyde, Kevin D.</mods:namePart>
<mods:affiliation>Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand &amp; CAS Key Laboratory for Plant Biodiversity and Biogeography of East Asia (KLPB), Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan, China &amp; Innovative Institute of Plant Health, Zhongkai University of Agriculture and Enginnering, Haizhu District, Guangzhou 510225, China</mods:affiliation>
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<mods:title>Biodiversity Data Journal</mods:title>
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<mods:date>2021</mods:date>
<mods:detail type="pubDate">
<mods:number>2021-01-18</mods:number>
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<mods:number>9</mods:number>
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<mods:identifier type="Pensoft-UUID">2E56CD86B4425E9A8825FFF492BF19C8</mods:identifier>
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<treatment LSID="urn:lsid:plazi:treatment:29E4C2F05ADA5F9389E0A48495626C05" httpUri="http://treatment.plazi.org/id/29E4C2F05ADA5F9389E0A48495626C05" lastPageNumber="59648" pageId="0" pageNumber="59648">
<subSubSection pageId="0" pageNumber="59648" type="nomenclature">
<paragraph pageId="0" pageNumber="59648">
<taxonomicName LSID="29E4C2F0-5ADA-5F93-89E0-A48495626C05" authority="(Westend.) Phookamsak., Wanas., S. K. Huang, Camporesi &amp; K. D. Hyde (2017)" authorityName="Phookamsak., Wanas., S. K. Huang, Camporesi &amp; K. D. Hyde" authorityYear="2017" baseAuthorityName="Westend." baseAuthorityYear="2017" class="Ascomycetes" family="Phaeosphaeriaceae" genus="Pseudoophiobolus" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="Pseudoophiobolus mathieui" order="Pleosporales" pageId="0" pageNumber="59648" phylum="Ascomycota" rank="species" species="mathieui">Pseudoophiobolus mathieui (Westend.) Phookamsak., Wanas., S.K. Huang, Camporesi &amp; K.D. Hyde (2017)</taxonomicName>
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="59648" type="reference_group">
<paragraph pageId="0" pageNumber="59648">
<taxonomicName authorityName="Phookamsak., Wanas., S. K. Huang, Camporesi &amp; K. D. Hyde" authorityYear="2017" baseAuthorityName="Westend." class="Ascomycetes" family="Phaeosphaeriaceae" genus="Pseudoophiobolus" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="Pseudoophiobolus mathieui" order="Pleosporales" pageId="0" pageNumber="59648" phylum="Ascomycota" rank="species" species="mathieui">Pseudoophiobolus mathieui</taxonomicName>
<taxonomicName class="Dothideomycetes" family="Pleosporaceae" genus="Sphaeria" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="Sphaeria mathieui" order="Pleosporales" pageId="0" pageNumber="59648" phylum="Ascomycota" rank="species" species="mathieui">
<emphasis italics="true" pageId="0" pageNumber="59648">Sphaeria mathieui</emphasis>
</taxonomicName>
</paragraph>
<paragraph pageId="0" pageNumber="59648">
<taxonomicName class="Dothideomycetes" family="Pleosporaceae" genus="Sphaeria" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="Sphaeria mathieui" order="Pleosporales" pageId="0" pageNumber="59648" phylum="Ascomycota" rank="species" species="mathieui">Sphaeria mathieui</taxonomicName>
Basionym:
<taxonomicName class="Dothideomycetes" family="Pleosporaceae" genus="Sphaeria" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="Sphaeria mathieui" order="Pleosporales" pageId="0" pageNumber="59648" phylum="Ascomycota" rank="species" species="mathieui">
<emphasis italics="true" pageId="0" pageNumber="59648">Sphaeria mathieui</emphasis>
</taxonomicName>
Westend., Bull. Acad. R. Sci. Belg., Cl. Sci.,
<normalizedToken originalValue="sér">ser</normalizedToken>
. 2: no. 5 (1859)
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="59648" type="materials_examined">
<paragraph pageId="0" pageNumber="59648">Materials</paragraph>
<paragraph pageId="0" pageNumber="59648">
<materialsCitation collectionCode="Mae Fah Luang University Herbarium (MFLU)" collectorName="Erio Camporesi" pageId="0" pageNumber="59648" specimenCount="1" typeStatus="Other material">
<emphasis bold="true" pageId="0" pageNumber="59648">Type status:</emphasis>
<typeStatus pageId="0" pageNumber="59648">Other material</typeStatus>
.
<emphasis bold="true" pageId="0" pageNumber="59648">Occurrence:</emphasis>
recordedBy:
<collectorName pageId="0" pageNumber="59648">Erio Camporesi</collectorName>
;
<emphasis bold="true" pageId="0" pageNumber="59648">Taxon:</emphasis>
kingdom: Fungi; phylum: Ascomycota; class: Dothideomycetes; order: Pleosporales; family: Phaeosphaeriaceae; genus: Pseudoophiobolus; specificEpithet: mathieui; taxonRank: species;
<emphasis bold="true" pageId="0" pageNumber="59648">Location:</emphasis>
stateProvince: Province of Ravenna; county: Italy; municipality: near Brisighella;
<emphasis bold="true" pageId="0" pageNumber="59648">Identification:</emphasis>
identifiedBy:
<determinerName pageId="0" pageNumber="59648">S.N. Wijesinghe</determinerName>
;
<emphasis bold="true" pageId="0" pageNumber="59648">Event:</emphasis>
year: 2018; month: 9; day: 10; habitat: on a dead areail stem of Artemisia sp. (Asterales, Asteraceae); fieldNotes: Terrestrial;
<emphasis bold="true" pageId="0" pageNumber="59648">Record Level:</emphasis>
institutionID: MFLU 18-1907; institutionCode:
<collectionCode pageId="0" pageNumber="59648">Mae Fah Luang University Herbarium (MFLU)</collectionCode>
; ownerInstitutionCode: IT4031
</materialsCitation>
<materialsCitation collectionCode="Mae Fah Luang Culture Collection (MFLUCC)" pageId="0" pageNumber="59648" specimenCount="1" typeStatus="Other material">
<emphasis bold="true" pageId="0" pageNumber="59648">Type status:</emphasis>
<typeStatus pageId="0" pageNumber="59648">Other material</typeStatus>
.
<emphasis bold="true" pageId="0" pageNumber="59648">Record Level:</emphasis>
type: living culture; collectionID: MFLUCC 20-0150; collectionCode:
<collectionCode pageId="0" pageNumber="59648">Mae Fah Luang Culture Collection (MFLUCC)</collectionCode>
</materialsCitation>
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="59648" type="description">
<paragraph pageId="0" pageNumber="59648">Description</paragraph>
<paragraph pageId="0" pageNumber="59648">
Saprobic on dead aerial stem of
<taxonomicName class="Magnoliopsida" family="Asteraceae" genus="Artemisia" higherTaxonomySource="CoL,IPNI" kingdom="Plantae" lsidName="Artemisia" order="Asterales" pageId="0" pageNumber="59648" phylum="Tracheophyta" rank="genus">
<emphasis italics="true" pageId="0" pageNumber="59648">Artemisia</emphasis>
</taxonomicName>
sp. (
<taxonomicName class="Magnoliopsida" higherTaxonomySource="CoL,IPNI" kingdom="Plantae" lsidName="" order="Asterales" pageId="0" pageNumber="59648" phylum="Tracheophyta" rank="order">Asterales</taxonomicName>
,
<taxonomicName class="Magnoliopsida" family="Asteraceae" higherTaxonomySource="CoL,IPNI" kingdom="Plantae" lsidName="" order="Asterales" pageId="0" pageNumber="59648" phylum="Tracheophyta" rank="family">Asteraceae</taxonomicName>
).
<emphasis bold="true" pageId="0" pageNumber="59648">Sexual morph</emphasis>
:
<emphasis italics="true" pageId="0" pageNumber="59648">Ascomata</emphasis>
(Fig.
<figureCitation captionStart="Figure 4" captionStartId="F6199384" captionText="Figure 4. Pseudoophiobolus mathieui (MFLU 18 - 1907). a-b. Ascomata on dead host surface of Artemisia sp. (Asterales, Asteraceae). c. Section of an ascoma. d. Close-up of ostiole. e. Peridium. f. Pseudoparaphyses. g-j. Asci. k-m. Ascospores. n. Ascospore with a swollen point (arrow). o-p. Colonies on PDA from upper (o) to lower (p) sides. Scale bars: b, d = 100 µm, c, f = 50 µm, e, g, h, l, m = 20 µm, i, j = 10 µm, n = 5 µm." figureDoi="10.3897/BDJ.9.e59648.figure4" httpUri="https://binary.pensoft.net/fig/460262" pageId="0" pageNumber="59648">4</figureCitation>
a-b, c - with ostiole) 170-300
<normalizedToken originalValue="×">x</normalizedToken>
140-250
<normalizedToken originalValue="µm">µm</normalizedToken>
(x¯ = 200
<normalizedToken originalValue="×">x</normalizedToken>
177
<normalizedToken originalValue="µm">µm</normalizedToken>
, n = 10), solitary, scattered, dark brown to black, semi-immersed to erumpent, sessile, globose to subglobose, uni-loculate, coriaceous, ostiolate and papillate.
<taxonomicName genus="Papilla" lsidName="Papilla" pageId="0" pageNumber="59648" rank="genus">
<emphasis italics="true" pageId="0" pageNumber="59648">Papilla</emphasis>
</taxonomicName>
(Fig.
<figureCitation captionStart="Figure 4" captionStartId="F6199384" captionText="Figure 4. Pseudoophiobolus mathieui (MFLU 18 - 1907). a-b. Ascomata on dead host surface of Artemisia sp. (Asterales, Asteraceae). c. Section of an ascoma. d. Close-up of ostiole. e. Peridium. f. Pseudoparaphyses. g-j. Asci. k-m. Ascospores. n. Ascospore with a swollen point (arrow). o-p. Colonies on PDA from upper (o) to lower (p) sides. Scale bars: b, d = 100 µm, c, f = 50 µm, e, g, h, l, m = 20 µm, i, j = 10 µm, n = 5 µm." figureDoi="10.3897/BDJ.9.e59648.figure4" httpUri="https://binary.pensoft.net/fig/460262" pageId="0" pageNumber="59648">4</figureCitation>
d) 70-150
<normalizedToken originalValue="×">x</normalizedToken>
60-120
<normalizedToken originalValue="µm">µm</normalizedToken>
, mammiform to oblong, with a rounded to truncate apex, thick walled, composed of several layers, brown to dark brown cells of
<emphasis italics="true" pageId="0" pageNumber="59648">textura angularis</emphasis>
, ostiole central, single and without periphyses.
<emphasis italics="true" pageId="0" pageNumber="59648">Peridium</emphasis>
(Fig.
<figureCitation captionStart="Figure 4" captionStartId="F6199384" captionText="Figure 4. Pseudoophiobolus mathieui (MFLU 18 - 1907). a-b. Ascomata on dead host surface of Artemisia sp. (Asterales, Asteraceae). c. Section of an ascoma. d. Close-up of ostiole. e. Peridium. f. Pseudoparaphyses. g-j. Asci. k-m. Ascospores. n. Ascospore with a swollen point (arrow). o-p. Colonies on PDA from upper (o) to lower (p) sides. Scale bars: b, d = 100 µm, c, f = 50 µm, e, g, h, l, m = 20 µm, i, j = 10 µm, n = 5 µm." figureDoi="10.3897/BDJ.9.e59648.figure4" httpUri="https://binary.pensoft.net/fig/460262" pageId="0" pageNumber="59648">4</figureCitation>
e) 15-35
<normalizedToken originalValue="µm">µm</normalizedToken>
(x¯ = 20
<normalizedToken originalValue="µm">µm</normalizedToken>
, n = 15), brown to black, thick-walled, pseudoparenchymatous cells, composed of 4-6 cell layers, outer layers composed of dark brown loosely packed cells of
<emphasis italics="true" pageId="0" pageNumber="59648">textura angularis</emphasis>
, inner layers composed of light brown to hyaline flattened cells of textura prismatica.
<emphasis italics="true" pageId="0" pageNumber="59648">Hamathecium</emphasis>
comprising numerous, 1.5-2.5
<normalizedToken originalValue="µm">µm</normalizedToken>
wide (x¯ = 2
<normalizedToken originalValue="µm">µm</normalizedToken>
, n = 15), filamentous, distinctly septate, cellular pseudoparaphyses (Fig.
<figureCitation captionStart="Figure 4" captionStartId="F6199384" captionText="Figure 4. Pseudoophiobolus mathieui (MFLU 18 - 1907). a-b. Ascomata on dead host surface of Artemisia sp. (Asterales, Asteraceae). c. Section of an ascoma. d. Close-up of ostiole. e. Peridium. f. Pseudoparaphyses. g-j. Asci. k-m. Ascospores. n. Ascospore with a swollen point (arrow). o-p. Colonies on PDA from upper (o) to lower (p) sides. Scale bars: b, d = 100 µm, c, f = 50 µm, e, g, h, l, m = 20 µm, i, j = 10 µm, n = 5 µm." figureDoi="10.3897/BDJ.9.e59648.figure4" httpUri="https://binary.pensoft.net/fig/460262" pageId="0" pageNumber="59648">4</figureCitation>
f) with guttules, slightly constricted at the septa, anastomosing at the apex, embedded in a hyaline gelatinous matrix.
<emphasis italics="true" pageId="0" pageNumber="59648">Asci</emphasis>
(Fig.
<figureCitation captionStart="Figure 4" captionStartId="F6199384" captionText="Figure 4. Pseudoophiobolus mathieui (MFLU 18 - 1907). a-b. Ascomata on dead host surface of Artemisia sp. (Asterales, Asteraceae). c. Section of an ascoma. d. Close-up of ostiole. e. Peridium. f. Pseudoparaphyses. g-j. Asci. k-m. Ascospores. n. Ascospore with a swollen point (arrow). o-p. Colonies on PDA from upper (o) to lower (p) sides. Scale bars: b, d = 100 µm, c, f = 50 µm, e, g, h, l, m = 20 µm, i, j = 10 µm, n = 5 µm." figureDoi="10.3897/BDJ.9.e59648.figure4" httpUri="https://binary.pensoft.net/fig/460262" pageId="0" pageNumber="59648">4</figureCitation>
g-j) 100-150
<normalizedToken originalValue="×">x</normalizedToken>
6-9
<normalizedToken originalValue="µm">µm</normalizedToken>
(x¯ = 132
<normalizedToken originalValue="×">x</normalizedToken>
8
<normalizedToken originalValue="µm">µm</normalizedToken>
, n = 15), 8-spored, bitunicate, fissitunicate, cylindrical to cylindrical-clavate, short furcate pedicel, apically rounded, well-developed ocular chamber.
<emphasis italics="true" pageId="0" pageNumber="59648">Ascospores</emphasis>
(Fig.
<figureCitation captionStart="Figure 4" captionStartId="F6199384" captionText="Figure 4. Pseudoophiobolus mathieui (MFLU 18 - 1907). a-b. Ascomata on dead host surface of Artemisia sp. (Asterales, Asteraceae). c. Section of an ascoma. d. Close-up of ostiole. e. Peridium. f. Pseudoparaphyses. g-j. Asci. k-m. Ascospores. n. Ascospore with a swollen point (arrow). o-p. Colonies on PDA from upper (o) to lower (p) sides. Scale bars: b, d = 100 µm, c, f = 50 µm, e, g, h, l, m = 20 µm, i, j = 10 µm, n = 5 µm." figureDoi="10.3897/BDJ.9.e59648.figure4" httpUri="https://binary.pensoft.net/fig/460262" pageId="0" pageNumber="59648">4</figureCitation>
k-m) 120-150
<normalizedToken originalValue="×">x</normalizedToken>
2-3
<normalizedToken originalValue="µm">µm</normalizedToken>
(x¯ = 131
<normalizedToken originalValue="×">x</normalizedToken>
2.8
<normalizedToken originalValue="µm">µm</normalizedToken>
, n = 25), fasciculate, lying parallel or spiral at the centre, scolecosporous, filiform or filamentous, narrowly rounded towards the ends, slightly swollen at the middle of 4th or 5th cell from the apex (Fig.
<figureCitation captionStart="Figure 4" captionStartId="F6199384" captionText="Figure 4. Pseudoophiobolus mathieui (MFLU 18 - 1907). a-b. Ascomata on dead host surface of Artemisia sp. (Asterales, Asteraceae). c. Section of an ascoma. d. Close-up of ostiole. e. Peridium. f. Pseudoparaphyses. g-j. Asci. k-m. Ascospores. n. Ascospore with a swollen point (arrow). o-p. Colonies on PDA from upper (o) to lower (p) sides. Scale bars: b, d = 100 µm, c, f = 50 µm, e, g, h, l, m = 20 µm, i, j = 10 µm, n = 5 µm." figureDoi="10.3897/BDJ.9.e59648.figure4" httpUri="https://binary.pensoft.net/fig/460262" pageId="0" pageNumber="59648">4</figureCitation>
n), yellowish to yellowish brown, 15-18 septate and not constricted at the septa, smooth-walled.
<emphasis bold="true" pageId="0" pageNumber="59648">Asexual morph</emphasis>
: Undetermined.
</paragraph>
<paragraph pageId="0" pageNumber="59648">
Culture characteristics: Ascospores germinating on PDA within 4 days, from single-spore isolation. Colonies (Fig.
<figureCitation captionStart="Figure 4" captionStartId="F6199384" captionText="Figure 4. Pseudoophiobolus mathieui (MFLU 18 - 1907). a-b. Ascomata on dead host surface of Artemisia sp. (Asterales, Asteraceae). c. Section of an ascoma. d. Close-up of ostiole. e. Peridium. f. Pseudoparaphyses. g-j. Asci. k-m. Ascospores. n. Ascospore with a swollen point (arrow). o-p. Colonies on PDA from upper (o) to lower (p) sides. Scale bars: b, d = 100 µm, c, f = 50 µm, e, g, h, l, m = 20 µm, i, j = 10 µm, n = 5 µm." figureDoi="10.3897/BDJ.9.e59648.figure4" httpUri="https://binary.pensoft.net/fig/460262" pageId="0" pageNumber="59648">4</figureCitation>
o-p) on PDA reaching 10-15 mm diam. after 14 days at 16°C, circular, entire edge, flat, dense, pale yellow in both upper and lower centres, white at the edges in both sides.
</paragraph>
<paragraph pageId="0" pageNumber="59648">GenBank accession numbers (ex-MFLUCC 20-0150): SSU = MT880290, ITS = MT880294, LSU = MT880292, TEF = MT901292</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="59648" type="notes">
<paragraph pageId="0" pageNumber="59648">Notes</paragraph>
<paragraph pageId="0" pageNumber="59648">
<taxonomicName authorityName="Phookamsak, Wanas. &amp; K.D.Hyde" authorityYear="2017" class="Ascomycetes" family="Phaeosphaeriaceae" genus="Pseudoophiobolus" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="Pseudoophiobolus" order="Pleosporales" pageId="0" pageNumber="59648" phylum="Ascomycota" rank="genus">
<emphasis italics="true" pageId="0" pageNumber="59648">Pseudoophiobolus</emphasis>
</taxonomicName>
was introduced by
<bibRefCitation author="Phookamsak, R." journalOrPublisher="Fungal Diversity" pageId="0" pageNumber="59648" pagination="299 - 339" refId="B6202386" refString="Phookamsak, R., Wanasinghe, D. N., Hongsanan, S., Phukhamsakda, C., Huang, S. K., Tennakoon, D. S., Norphanphoun, C., Camporesi, E., Bulgakov, T. S., Promputa, I., Mortimer, P. E., Xu, J. C., 2017. Towards a natural classification of Ophiobolus and ophiobolus-like taxa; introducing three novel genera Ophiobolopsis, Paraophiobolus and Pseudoophiobolus in Phaeosphaeriaceae (Pleosporales). Fungal Diversity 87: 299 - 339" title="Towards a natural classification of Ophiobolus and ophiobolus-like taxa; introducing three novel genera Ophiobolopsis, Paraophiobolus and Pseudoophiobolus in Phaeosphaeriaceae (Pleosporales)" volume="87" year="2017">Phookamsak et al. (2017)</bibRefCitation>
to accommodate
<taxonomicName class="Dothideomycetes" family="Phaeosphaeriaceae" genus="Ophiobolus" higherTaxonomySource="GBIF,CoL" kingdom="Fungi" lsidName="Ophiobolus" order="Pleosporales" pageId="0" pageNumber="59648" phylum="Ascomycota" rank="genus">
<emphasis italics="true" pageId="0" pageNumber="59648">Ophiobolus</emphasis>
</taxonomicName>
-like taxa, including
<taxonomicName class="Ascomycetes" family="Urticaceae" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="P. mathieui" order="Pleosporales" pageId="0" pageNumber="59648" phylum="Ascomycota" rank="species" species="mathieui">
<emphasis italics="true" pageId="0" pageNumber="59648">P. mathieui</emphasis>
</taxonomicName>
, characterised by ascospores that are subhyaline to pale yellowish or yellowish, with a swollen cell, lacking terminal appendages and not separating into part spores. Both the new Italian strain (MFLUCC 20-0150) and the previously-isolated ex-type strain of
<taxonomicName class="Ascomycetes" family="Urticaceae" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="P. mathieui" order="Pleosporales" pageId="0" pageNumber="59648" phylum="Ascomycota" rank="species" species="mathieui">
<emphasis italics="true" pageId="0" pageNumber="59648">P. mathieui</emphasis>
</taxonomicName>
(MFLUCC 17-1785) were collected from the Province of
<normalizedToken originalValue="Forlì-Cesena">Forli-Cesena</normalizedToken>
, on
<taxonomicName class="Magnoliopsida" family="Asteraceae" genus="Artemisia" higherTaxonomySource="CoL,IPNI" kingdom="Plantae" lsidName="Artemisia" order="Asterales" pageId="0" pageNumber="59648" phylum="Tracheophyta" rank="genus">
<emphasis italics="true" pageId="0" pageNumber="59648">Artemisia</emphasis>
</taxonomicName>
sp. (
<taxonomicName class="Magnoliopsida" higherTaxonomySource="CoL,IPNI" kingdom="Plantae" lsidName="" order="Asterales" pageId="0" pageNumber="59648" phylum="Tracheophyta" rank="order">Asterales</taxonomicName>
,
<taxonomicName class="Magnoliopsida" family="Asteraceae" higherTaxonomySource="CoL,IPNI" kingdom="Plantae" lsidName="" order="Asterales" pageId="0" pageNumber="59648" phylum="Tracheophyta" rank="family">Asteraceae</taxonomicName>
) and
<taxonomicName class="Magnoliopsida" family="Lamiaceae" genus="Salvia" higherTaxonomySource="IPNI" kingdom="Plantae" lsidName="Salvia" order="Lamiales" pageId="0" pageNumber="59648" phylum="Magnoliophyta" rank="genus">
<emphasis italics="true" pageId="0" pageNumber="59648">Salvia</emphasis>
</taxonomicName>
sp. (
<taxonomicName class="Magnoliopsida" higherTaxonomySource="IPNI" kingdom="Plantae" lsidName="" order="Lamiales" pageId="0" pageNumber="59648" phylum="Magnoliophyta" rank="order">Lamiales</taxonomicName>
,
<taxonomicName class="Magnoliopsida" family="Lamiaceae" higherTaxonomySource="IPNI" kingdom="Plantae" lsidName="" order="Lamiales" pageId="0" pageNumber="59648" phylum="Magnoliophyta" rank="family">Lamiaceae</taxonomicName>
), respectively. Further records were reported for the same Province on
<taxonomicName class="Magnoliopsida" family="Lamiaceae" genus="Origanum" higherTaxonomySource="IPNI" kingdom="Plantae" lsidName="Origanum vulgare" order="Lamiales" pageId="0" pageNumber="59648" phylum="Magnoliophyta" rank="species" species="vulgare">
<emphasis italics="true" pageId="0" pageNumber="59648">Origanum vulgare</emphasis>
</taxonomicName>
(
<taxonomicName class="Magnoliopsida" higherTaxonomySource="IPNI" kingdom="Plantae" lsidName="" order="Lamiales" pageId="0" pageNumber="59648" phylum="Magnoliophyta" rank="order">Lamiales</taxonomicName>
,
<taxonomicName class="Magnoliopsida" family="Lamiaceae" higherTaxonomySource="IPNI" kingdom="Plantae" lsidName="" order="Lamiales" pageId="0" pageNumber="59648" phylum="Magnoliophyta" rank="family">Lamiaceae</taxonomicName>
) and
<taxonomicName class="Magnoliopsida" family="Fabaceae" genus="Ononis" higherTaxonomySource="IPNI" kingdom="Plantae" lsidName="Ononis spinosa" order="Fabales" pageId="0" pageNumber="59648" phylum="Magnoliophyta" rank="species" species="spinosa">
<emphasis italics="true" pageId="0" pageNumber="59648">Ononis spinosa</emphasis>
</taxonomicName>
(
<taxonomicName class="Magnoliopsida" higherTaxonomySource="IPNI" kingdom="Plantae" lsidName="" order="Fabales" pageId="0" pageNumber="59648" phylum="Magnoliophyta" rank="order">Fabales</taxonomicName>
,
<taxonomicName class="Magnoliopsida" family="Fabaceae" higherTaxonomySource="IPNI" kingdom="Plantae" lsidName="" order="Fabales" pageId="0" pageNumber="59648" phylum="Magnoliophyta" rank="family">Fabaceae</taxonomicName>
) (
<bibRefCitation author="Phookamsak, R." journalOrPublisher="Fungal Diversity" pageId="0" pageNumber="59648" pagination="299 - 339" refId="B6202386" refString="Phookamsak, R., Wanasinghe, D. N., Hongsanan, S., Phukhamsakda, C., Huang, S. K., Tennakoon, D. S., Norphanphoun, C., Camporesi, E., Bulgakov, T. S., Promputa, I., Mortimer, P. E., Xu, J. C., 2017. Towards a natural classification of Ophiobolus and ophiobolus-like taxa; introducing three novel genera Ophiobolopsis, Paraophiobolus and Pseudoophiobolus in Phaeosphaeriaceae (Pleosporales). Fungal Diversity 87: 299 - 339" title="Towards a natural classification of Ophiobolus and ophiobolus-like taxa; introducing three novel genera Ophiobolopsis, Paraophiobolus and Pseudoophiobolus in Phaeosphaeriaceae (Pleosporales)" volume="87" year="2017">Phookamsak et al. 2017</bibRefCitation>
). Characteristics of our material resemble the holotype (
<bibRefCitation author="Phookamsak, R." journalOrPublisher="Fungal Diversity" pageId="0" pageNumber="59648" pagination="299 - 339" refId="B6202386" refString="Phookamsak, R., Wanasinghe, D. N., Hongsanan, S., Phukhamsakda, C., Huang, S. K., Tennakoon, D. S., Norphanphoun, C., Camporesi, E., Bulgakov, T. S., Promputa, I., Mortimer, P. E., Xu, J. C., 2017. Towards a natural classification of Ophiobolus and ophiobolus-like taxa; introducing three novel genera Ophiobolopsis, Paraophiobolus and Pseudoophiobolus in Phaeosphaeriaceae (Pleosporales). Fungal Diversity 87: 299 - 339" title="Towards a natural classification of Ophiobolus and ophiobolus-like taxa; introducing three novel genera Ophiobolopsis, Paraophiobolus and Pseudoophiobolus in Phaeosphaeriaceae (Pleosporales)" volume="87" year="2017">Phookamsak et al. 2017</bibRefCitation>
). The holotype of
<taxonomicName class="Ascomycetes" family="Urticaceae" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="P. mathieui" order="Pleosporales" pageId="0" pageNumber="59648" phylum="Ascomycota" rank="species" species="mathieui">
<emphasis italics="true" pageId="0" pageNumber="59648">P. mathieui</emphasis>
</taxonomicName>
(MFLUCC 17-1785) and our newly-isolated strain (MFLUCC 20-0150) were similar in ascomata, ostiole, peridium and asci, but the ascomatal ostiole of MFLUCC 20-0150 was composed of cells of
<emphasis italics="true" pageId="0" pageNumber="59648">textura angularis</emphasis>
, whereas, in MFLUCC 17-1785, the cells were of
<emphasis italics="true" pageId="0" pageNumber="59648">textura angularis</emphasis>
to
<emphasis italics="true" pageId="0" pageNumber="59648">textura prismatica</emphasis>
(Fig.
<figureCitation captionStart="Figure 4" captionStartId="F6199384" captionText="Figure 4. Pseudoophiobolus mathieui (MFLU 18 - 1907). a-b. Ascomata on dead host surface of Artemisia sp. (Asterales, Asteraceae). c. Section of an ascoma. d. Close-up of ostiole. e. Peridium. f. Pseudoparaphyses. g-j. Asci. k-m. Ascospores. n. Ascospore with a swollen point (arrow). o-p. Colonies on PDA from upper (o) to lower (p) sides. Scale bars: b, d = 100 µm, c, f = 50 µm, e, g, h, l, m = 20 µm, i, j = 10 µm, n = 5 µm." figureDoi="10.3897/BDJ.9.e59648.figure4" httpUri="https://binary.pensoft.net/fig/460262" pageId="0" pageNumber="59648">4</figureCitation>
).
</paragraph>
<paragraph pageId="0" pageNumber="59648">
From a comparison of ITS and LSU sequences between
<taxonomicName class="Ascomycetes" family="Urticaceae" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="P. mathieui" order="Pleosporales" pageId="0" pageNumber="59648" phylum="Ascomycota" rank="species" species="mathieui">
<emphasis italics="true" pageId="0" pageNumber="59648">P. mathieui</emphasis>
</taxonomicName>
(type) and MFLUCC 20-0150 strain, both were identical. However, seven nucleotide differences (1.13%) were found between the TEF sequences of two strains. Following the integrative taxonomic approach with both morphological data and molecular phylogenetic analyses, we conclude that our new collection is
<taxonomicName authorityName="Phookamsak., Wanas., S. K. Huang, Camporesi &amp; K. D. Hyde" authorityYear="2017" baseAuthorityName="Westend." class="Ascomycetes" family="Phaeosphaeriaceae" genus="Pseudoophiobolus" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="Pseudoophiobolus mathieui" order="Pleosporales" pageId="0" pageNumber="59648" phylum="Ascomycota" rank="species" species="mathieui">
<emphasis italics="true" pageId="0" pageNumber="59648">Pseudoophiobolus mathieui</emphasis>
</taxonomicName>
and represents a new host record on
<taxonomicName class="Magnoliopsida" family="Asteraceae" genus="Artemisia" higherTaxonomySource="CoL,IPNI" kingdom="Plantae" lsidName="Artemisia" order="Asterales" pageId="0" pageNumber="59648" phylum="Tracheophyta" rank="genus">
<emphasis italics="true" pageId="0" pageNumber="59648">Artemisia</emphasis>
</taxonomicName>
sp. (
<taxonomicName class="Magnoliopsida" higherTaxonomySource="CoL,IPNI" kingdom="Plantae" lsidName="" order="Asterales" pageId="0" pageNumber="59648" phylum="Tracheophyta" rank="order">Asterales</taxonomicName>
,
<taxonomicName class="Magnoliopsida" family="Asteraceae" higherTaxonomySource="CoL,IPNI" kingdom="Plantae" lsidName="" order="Asterales" pageId="0" pageNumber="59648" phylum="Tracheophyta" rank="family">Asteraceae</taxonomicName>
).
</paragraph>
</subSubSection>
</treatment>
</document>