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<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.94.95751" ID-Pensoft-Pub="1314-4049-94-51" ID-Pensoft-UUID="1E443200E6585C66B1D669737794361F" ModsDocID="1314-4049-94-51" checkinTime="1670952471273" checkinUser="pensoft" docAuthor="Lu, Li, Karunarathna, Samantha C., Dai, Dong-qin, Jayawardena, Ruvishika S., Suwannarach, Nakarin &amp; Tibpromma, Saowaluck" docDate="2022" docId="1B95946CF0165EA6988D8E1715C78A93" docLanguage="en" docName="MycoKeys 94: 51-71" docOrigin="MycoKeys 94" docPubDate="2022-12-12" docSource="http://dx.doi.org/10.3897/mycokeys.94.95751" docTitle="Nigrograna puerensis L. Lu &amp; Tibpromma 2022, sp. nov." docType="treatment" docVersion="1" id="1E443200E6585C66B1D669737794361F" lastPageNumber="51" masterDocId="1E443200E6585C66B1D669737794361F" masterDocTitle="Three new species of Nigrograna (Dothideomycetes, Pleosporales) associated with Arabica coffee from Yunnan Province, China" masterLastPageNumber="71" masterPageNumber="51" pageNumber="51" updateTime="1670952471273" updateUser="pensoft">
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<mods:titleInfo>
<mods:title>Three new species of Nigrograna (Dothideomycetes, Pleosporales) associated with Arabica coffee from Yunnan Province, China</mods:title>
</mods:titleInfo>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Lu, Li</mods:namePart>
<mods:affiliation>Center for Yunnan Plateau Biological Resources Protection and Utilization, College of Biological Resource and Food Engineering, Qujing Normal University, Qujing, Yunnan 655011, China &amp; Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Karunarathna, Samantha C.</mods:namePart>
<mods:nameIdentifier type="ORCID">https://orcid.org/0000-0001-7080-0781</mods:nameIdentifier>
<mods:affiliation>Center for Yunnan Plateau Biological Resources Protection and Utilization, College of Biological Resource and Food Engineering, Qujing Normal University, Qujing, Yunnan 655011, China</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Dai, Dong-qin</mods:namePart>
<mods:nameIdentifier type="ORCID">https://orcid.org/0000-0001-8935-8807</mods:nameIdentifier>
<mods:affiliation>Center for Yunnan Plateau Biological Resources Protection and Utilization, College of Biological Resource and Food Engineering, Qujing Normal University, Qujing, Yunnan 655011, China</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Jayawardena, Ruvishika S.</mods:namePart>
<mods:affiliation>Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Suwannarach, Nakarin</mods:namePart>
<mods:nameIdentifier type="ORCID">https://orcid.org/0000-0002-2653-1913</mods:nameIdentifier>
<mods:affiliation>School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand</mods:affiliation>
<mods:nameIdentifier type="email">suwan.462@gmail.com</mods:nameIdentifier>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Tibpromma, Saowaluck</mods:namePart>
<mods:affiliation>Center for Yunnan Plateau Biological Resources Protection and Utilization, College of Biological Resource and Food Engineering, Qujing Normal University, Qujing, Yunnan 655011, China</mods:affiliation>
<mods:nameIdentifier type="email">saowaluckfai@gmail.com</mods:nameIdentifier>
</mods:name>
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<mods:title>MycoKeys</mods:title>
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<mods:part>
<mods:date>2022</mods:date>
<mods:detail type="pubDate">
<mods:number>2022-12-12</mods:number>
</mods:detail>
<mods:detail type="volume">
<mods:number>94</mods:number>
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<mods:start>51</mods:start>
<mods:end>71</mods:end>
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<mods:location>
<mods:url>http://dx.doi.org/10.3897/mycokeys.94.95751</mods:url>
</mods:location>
<mods:classification>journal article</mods:classification>
<mods:identifier type="DOI">http://dx.doi.org/10.3897/mycokeys.94.95751</mods:identifier>
<mods:identifier type="Pensoft-Pub">1314-4049-94-51</mods:identifier>
<mods:identifier type="Pensoft-UUID">1E443200E6585C66B1D669737794361F</mods:identifier>
</mods:mods>
<treatment LSID="urn:lsid:plazi:treatment:1B95946CF0165EA6988D8E1715C78A93" httpUri="http://treatment.plazi.org/id/1B95946CF0165EA6988D8E1715C78A93" lastPageNumber="51" pageId="0" pageNumber="51">
<subSubSection pageId="0" pageNumber="51" type="nomenclature">
<paragraph pageId="0" pageNumber="51">
<taxonomicName LSID="1B95946C-F016-5EA6-988D-8E1715C78A93" authority="L. Lu &amp; Tibpromma" authorityName="L. Lu &amp; Tibpromma" authorityYear="2022" class="Dothideomycetes" family="Biatriosporaceae" genus="Nigrograna" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Nigrograna puerensis" order="Pleosporales" pageId="0" pageNumber="51" phylum="Ascomycota" rank="species" species="puerensis" status="sp. nov.">Nigrograna puerensis L. Lu &amp; Tibpromma</taxonomicName>
<taxonomicNameLabel pageId="0" pageNumber="51">sp. nov.</taxonomicNameLabel>
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="51" type="description">
<paragraph pageId="0" pageNumber="51">
<figureCitation captionStart="Figure 3" captionStartId="F3" captionText="Figure 3. Nigrograna puerensis (ZHKU 22 - 0122, holotype) a, b ascomata observed on host substrate c a vertical section through an ascoma d peridium e hamathecium f-j asci k germinated ascospore l-p ascospores q culture on PDA from above and reverse. Scale bars: 50 μm (c); 30 μm (d); 15 μm (e-k); 5 μm (l-p)." figureDoi="10.3897/mycokeys.94.95751.figure3" httpUri="https://binary.pensoft.net/fig/781090" pageId="0" pageNumber="51">Fig. 3</figureCitation>
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="51" type="etymology">
<paragraph pageId="0" pageNumber="51">Etymology.</paragraph>
<paragraph pageId="0" pageNumber="51">
The specific epithet &quot;
<taxonomicName authorityName="L. Lu &amp; Tibpromma" authorityYear="2022" class="Dothideomycetes" family="Nigrogranaceae" genus="Nigrograna" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Nigrograna puerensis" order="Pleosporales" pageId="0" pageNumber="51" phylum="Ascomycota" rank="species" species="puerensis">
<emphasis italics="true" pageId="0" pageNumber="51">Nigrograna puerensis</emphasis>
</taxonomicName>
&quot; refers to the location
<normalizedToken originalValue="Puer">Pu'er</normalizedToken>
City, where the type species was collected.
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="51" type="holotype">
<paragraph pageId="0" pageNumber="51">Holotype.</paragraph>
<paragraph pageId="0" pageNumber="51">ZHKU 22-0122.</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="51" type="description">
<paragraph pageId="0" pageNumber="51">Description.</paragraph>
<paragraph pageId="0" pageNumber="51">
<emphasis bold="true" italics="true" pageId="0" pageNumber="51">Saprobic</emphasis>
on decaying branch of
<taxonomicName class="Magnoliopsida" family="Rubiaceae" genus="Coffea" kingdom="Plantae" lsidName="Coffea arabica" order="Gentianales" pageId="0" pageNumber="51" phylum="Tracheophyta" rank="species" species="arabica">
<emphasis italics="true" pageId="0" pageNumber="51">Coffea arabica</emphasis>
</taxonomicName>
.
<emphasis bold="true" pageId="0" pageNumber="51">
Sexual morph:
<emphasis italics="true" pageId="0" pageNumber="51">Ascomata</emphasis>
</emphasis>
90-180
<normalizedToken originalValue="µm">µm</normalizedToken>
high, 90-150
<normalizedToken originalValue="μm">μm</normalizedToken>
wide (x̄ = 138
<normalizedToken originalValue="×">x</normalizedToken>
115
<normalizedToken originalValue="μm">μm</normalizedToken>
, n = 10), immersed, with only ostiolar necks visible on the host surface or erumpent, solitary, subglobose to ellipsoid, dark brown (#6e5031).
<emphasis bold="true" italics="true" pageId="0" pageNumber="51">Peridium</emphasis>
10-15
<normalizedToken originalValue="μm">μm</normalizedToken>
wide (x̄ = 13
<normalizedToken originalValue="μm">μm</normalizedToken>
, n = 15), outer layer consists of 2-3 layers of
<emphasis bold="true" italics="true" pageId="0" pageNumber="51">textura prismatica</emphasis>
, brown (#937463) and thick-walled cells, inner layer hyaline with thin-walled cells.
<emphasis bold="true" italics="true" pageId="0" pageNumber="51">Hamathecium</emphasis>
composed of numerous, 1.5-2
<normalizedToken originalValue="µm">µm</normalizedToken>
wide (x̄ = 1.8
<normalizedToken originalValue="μm">μm</normalizedToken>
, n = 20), filamentous, hyaline, septate, pseudoparaphyse.
<emphasis bold="true" italics="true" pageId="0" pageNumber="51">Asci</emphasis>
50-80
<normalizedToken originalValue="×">x</normalizedToken>
8-11
<normalizedToken originalValue="μm">μm</normalizedToken>
(x̄ = 66
<normalizedToken originalValue="×">x</normalizedToken>
9.5
<normalizedToken originalValue="μm">μm</normalizedToken>
, n = 20), 8-spored, bitunicate, fissitunicate, cylindrical to clavate, short pedicellate, apically rounded, with poorly developed ocular chamber.
<emphasis bold="true" italics="true" pageId="0" pageNumber="51">Ascospores</emphasis>
15-18
<normalizedToken originalValue="×">x</normalizedToken>
4-5
<normalizedToken originalValue="μm">μm</normalizedToken>
, (x̄ = 16
<normalizedToken originalValue="×">x</normalizedToken>
4.5
<normalizedToken originalValue="μm">μm</normalizedToken>
, n = 30), uni- to bi-seriately arranged, fusoid, apical cell and basal cell acute, and apical cell slightly wider than basal cell, straight or slightly curved, 1-septate, constricted at septum, guttulate, hyaline to yellow-brownish (#daceb8) when young, brownish (#937463) when mature.
<emphasis bold="true" pageId="0" pageNumber="51">Asexual morph</emphasis>
: Undetermined.
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="51" type="description">
<paragraph pageId="0" pageNumber="51">Culture characteristics.</paragraph>
<paragraph pageId="0" pageNumber="51">On PDA, colonies reached up to 4 cm diam. after two months at room temperature (22-26 °C). Colony dense, circular, slightly raised at the center, surface with white aerial mycelium, fluffy, with a serrate edge, grayish (#c9bfb3) to dark brown (#6e5031) from center to edge, reverse dark green (#3a4543) to dark brown (#6e5031).</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="51" type="materials_examined">
<paragraph pageId="0" pageNumber="51">Material examined.</paragraph>
<paragraph pageId="0" pageNumber="51">
<materialsCitation accessionNumber="OP450969, OP450975, OP450983, OP432249, OP450970, OP450976, OP450984, OP432250" collectingDate="2021-09-16" country="China" elevation="1016" latitude="22.600555" location="Pu'er City" longLatPrecision="21" longitude="101.01639" specimenCount="1" stateProvince="Yunnan Province" typeStatus="holotype">
<location LSID="urn:lsid:plazi:treatment:1B95946CF0165EA6988D8E1715C78A93:78D15B7027398E62D606BB114AA95D9C" country="China" latitude="22.600555" longLatPrecision="21" longitude="101.01639" name="Pu'er City" stateProvince="Yunnan Province">
<normalizedToken originalValue="Puer">Pu'er</normalizedToken>
City
</location>
,
<collectingRegion country="China" name="Yunnan">Yunnan Province</collectingRegion>
,
<collectingCountry name="China">China</collectingCountry>
, on a decaying branch of
<taxonomicName class="Magnoliopsida" family="Rubiaceae" genus="Coffea" kingdom="Plantae" lsidName="Coffea arabica" order="Gentianales" pageId="0" pageNumber="51" phylum="Tracheophyta" rank="species" species="arabica">
<emphasis italics="true" pageId="0" pageNumber="51">Coffea arabica</emphasis>
</taxonomicName>
, (
<geoCoordinate degrees="22" direction="north" minutes="36" orientation="latitude" precision="15" seconds="2" value="22.600555">22°36'2&quot;N</geoCoordinate>
,
<geoCoordinate degrees="101" direction="east" minutes="0" orientation="longitude" precision="15" seconds="59" value="101.01639">101°0'59&quot;E</geoCoordinate>
,
<quantity metricMagnitude="3" metricUnit="m" metricValue="1.01643" unit="m" value="1016.43">
<elevation metricMagnitude="3" metricUnit="m" metricValue="1.01643" unit="m" value="1016.43">1016.43 m</elevation>
</quantity>
),
<collectingDate value="2021-09-16">16 September 2021</collectingDate>
, LiLu, Puer 1-4 (ZHKU 22-0122,
<typeStatus>holotype</typeStatus>
), ZHKUCC 22-0212 = ZHKUCC 22-0213. GenBank number; ITS:
<accessionNumber httpUri="http://www.ncbi.nlm.nih.gov/nucleotide/OP450969">OP450969</accessionNumber>
, LSU:
<accessionNumber httpUri="http://www.ncbi.nlm.nih.gov/nucleotide/OP450975">OP450975</accessionNumber>
, SSU:
<accessionNumber httpUri="http://www.ncbi.nlm.nih.gov/nucleotide/OP450983">OP450983</accessionNumber>
,
<emphasis italics="true" pageId="0" pageNumber="51">tef</emphasis>
1-α:
<accessionNumber httpUri="http://www.ncbi.nlm.nih.gov/nucleotide/OP432249">OP432249</accessionNumber>
(ZHKUCC 22-0212, ex-type); ITS:
<accessionNumber httpUri="http://www.ncbi.nlm.nih.gov/nucleotide/OP450970">OP450970</accessionNumber>
, LSU:
<accessionNumber httpUri="http://www.ncbi.nlm.nih.gov/nucleotide/OP450976">OP450976</accessionNumber>
, SSU:
<accessionNumber httpUri="http://www.ncbi.nlm.nih.gov/nucleotide/OP450984">OP450984</accessionNumber>
,
<emphasis italics="true" pageId="0" pageNumber="51">tef</emphasis>
1-α:
<accessionNumber httpUri="http://www.ncbi.nlm.nih.gov/nucleotide/OP432250">OP432250</accessionNumber>
(ZHKUCC 22-0213)
</materialsCitation>
.
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="51" type="notes">
<paragraph pageId="0" pageNumber="51">Notes.</paragraph>
<paragraph pageId="0" pageNumber="51">
<taxonomicName authorityName="L. Lu &amp; Tibpromma" authorityYear="2022" class="Dothideomycetes" family="Nigrogranaceae" genus="Nigrograna" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Nigrograna puerensis" order="Pleosporales" pageId="0" pageNumber="51" phylum="Ascomycota" rank="species" species="puerensis">
<emphasis italics="true" pageId="0" pageNumber="51">Nigrograna puerensis</emphasis>
</taxonomicName>
clusters with
<taxonomicName lsidName="N. carollii" pageId="0" pageNumber="51" rank="species" species="carollii">
<emphasis italics="true" pageId="0" pageNumber="51">N. carollii</emphasis>
</taxonomicName>
with significant statistical support from ML 100% and BIPP 1.00. In morphology, our new strains best fit
<taxonomicName authorityName="J.de Gruyter, G.J.M.Verkley &amp; P.W.Crous" authorityYear="2012" class="Dothideomycetes" family="Nigrogranaceae" genus="Nigrograna" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Nigrograna" order="Pleosporales" pageId="0" pageNumber="51" phylum="Ascomycota" rank="genus">
<emphasis italics="true" pageId="0" pageNumber="51">Nigrograna</emphasis>
</taxonomicName>
by having immersed ascomata, clavate and short pedicellate asci, and pale to brown, fusoid to narrowly ellipsoid, and septate ascospores (
<bibRefCitation DOI="https://doi.org/10.1016/j.simyco.2016.09.002" author="Jaklitsch, WM" journalOrPublisher="Studies in Mycology" pageId="0" pageNumber="51" pagination="35 - 64" refId="B19" refString="Jaklitsch, WM, Voglmayr, H, 2016. Hidden diversity in Thyridaria and a new circumscription of the Thyridariaceae. Studies in Mycology 85 (1): 35 - 64, DOI: https://doi.org/10.1016/j.simyco.2016.09.002" title="Hidden diversity in Thyridaria and a new circumscription of the Thyridariaceae." url="https://doi.org/10.1016/j.simyco.2016.09.002" volume="85" year="2016">Jaklitsch and Voglmayr 2016</bibRefCitation>
;
<bibRefCitation DOI="https://doi.org/10.1007/s11557-020-01633-0" author="Zhang, JF" journalOrPublisher="Mycological Progress" pageId="0" pageNumber="51" pagination="1365 - 1375" refId="B56" refString="Zhang, JF, Liu, JK, Thambugala, KM, Yang, J, Meng, ZH, Liu, ZY, 2020. Two new species and a new record of Nigrograna (Nigrogranaceae, Pleosporales) from China and Thailand. Mycological Progress 19: 1365 - 1375, DOI: https://doi.org/10.1007/s11557-020-01633-0" title="Two new species and a new record of Nigrograna (Nigrogranaceae, Pleosporales) from China and Thailand." url="https://doi.org/10.1007/s11557-020-01633-0" volume="19" year="2020">Zhang et al. 2020</bibRefCitation>
). Blast search results of ITS, LSU and
<emphasis italics="true" pageId="0" pageNumber="51">tef</emphasis>
1-α sequence data revealed that our taxon (ZHKUCC 22-0212) is similar to
<taxonomicName lsidName="N. mackinnonii" pageId="0" pageNumber="51" rank="species" species="mackinnonii">
<emphasis italics="true" pageId="0" pageNumber="51">N. mackinnonii</emphasis>
</taxonomicName>
(96% MZ270697, 99% KJ605422, and 95% LT797087 respectively), while the similarity of SSU sequence to
<taxonomicName lsidName="N. carollii" pageId="0" pageNumber="51" rank="species" species="carollii">
<emphasis italics="true" pageId="0" pageNumber="51">N. carollii</emphasis>
</taxonomicName>
is as high as 99%. Based on nucleotide comparisons, our isolate (ZHKUCC 22-0212) differs from
<taxonomicName lsidName="N. carollii" pageId="0" pageNumber="51" rank="species" species="carollii">
<emphasis italics="true" pageId="0" pageNumber="51">N. carollii</emphasis>
</taxonomicName>
(CCF 4484) by 9/490 bp (1.8%) in ITS, 2/222 bp (1%) in LSU, 2/1306 bp (0.2%) in SSU, and 10/530 bp (2%) in
<emphasis italics="true" pageId="0" pageNumber="51">tef</emphasis>
1-α. Unfortunately, for
<taxonomicName lsidName="N. carollii" pageId="0" pageNumber="51" rank="species" species="carollii">
<emphasis italics="true" pageId="0" pageNumber="51">N. carollii</emphasis>
</taxonomicName>
, sufficient morphological data was not available to compare with our novel taxon which was isolated as an endophyte on living sapwood of wild
<taxonomicName class="Magnoliopsida" family="Euphorbiaceae" genus="Hevea" kingdom="Plantae" lsidName="Hevea brasiliensis" order="Malpighiales" pageId="0" pageNumber="51" phylum="Tracheophyta" rank="species" species="brasiliensis">
<emphasis italics="true" pageId="0" pageNumber="51">Hevea brasiliensis</emphasis>
</taxonomicName>
<normalizedToken originalValue="Müll">Muell</normalizedToken>
. Arg., and
<taxonomicName lsidName="N. mackinnonii" pageId="0" pageNumber="51" rank="species" species="mackinnonii">
<emphasis italics="true" pageId="0" pageNumber="51">N. mackinnonii</emphasis>
</taxonomicName>
which was isolated as a human pathogen (
<bibRefCitation author="de Gruyter, JH" journalOrPublisher="Mycosphere" pageId="0" pageNumber="51" refId="B8" refString="de Gruyter, JH, 2012. Revised taxonomy of Phoma and allied genera. Wageningen University and Research, 1-181." title="Revised taxonomy of Phoma and allied genera. Wageningen University and Research, 1 - 181." year="2012">de Gruyter 2012</bibRefCitation>
;
<bibRefCitation DOI="https://doi.org/10.1007/s00606-016-1350-2" author="Kolarik, M" journalOrPublisher="Plant Systematics and Evolution" pageId="0" pageNumber="51" pagination="35 - 50" refId="B23" refString="Kolarik, M, Spakowicz, DJ, Gazis, R, Shaw, J, Kubatova, A, Novakova, A, Chudickova, M, Forcina, GC, Kang, KW, Kelnarova, I, Skaltsas, D, 2017. Biatriospora (Ascomycota: Pleosporales) is an ecologically diverse genus including facultative marine fungi and endophytes with biotechnological potential. Plant Systematics and Evolution 303 (1): 35 - 50, DOI: https://doi.org/10.1007/s00606-016-1350-2" title="Biatriospora (Ascomycota: Pleosporales) is an ecologically diverse genus including facultative marine fungi and endophytes with biotechnological potential." url="https://doi.org/10.1007/s00606-016-1350-2" volume="303" year="2017">
<normalizedToken originalValue="Kolařík">Kolarik</normalizedToken>
et al. 2017
</bibRefCitation>
). In addition, the colony morphology of
<taxonomicName lsidName="N. carollii" pageId="0" pageNumber="51" rank="species" species="carollii">
<emphasis italics="true" pageId="0" pageNumber="51">N. carollii</emphasis>
</taxonomicName>
on PDA is described as colonies plane, effuse, and light gray (
<bibRefCitation DOI="https://doi.org/10.1007/s00606-016-1350-2" author="Kolarik, M" journalOrPublisher="Plant Systematics and Evolution" pageId="0" pageNumber="51" pagination="35 - 50" refId="B23" refString="Kolarik, M, Spakowicz, DJ, Gazis, R, Shaw, J, Kubatova, A, Novakova, A, Chudickova, M, Forcina, GC, Kang, KW, Kelnarova, I, Skaltsas, D, 2017. Biatriospora (Ascomycota: Pleosporales) is an ecologically diverse genus including facultative marine fungi and endophytes with biotechnological potential. Plant Systematics and Evolution 303 (1): 35 - 50, DOI: https://doi.org/10.1007/s00606-016-1350-2" title="Biatriospora (Ascomycota: Pleosporales) is an ecologically diverse genus including facultative marine fungi and endophytes with biotechnological potential." url="https://doi.org/10.1007/s00606-016-1350-2" volume="303" year="2017">
<normalizedToken originalValue="Kolařík">Kolarik</normalizedToken>
et al. 2017
</bibRefCitation>
), while
<taxonomicName lsidName="N. puerensis" pageId="0" pageNumber="51" rank="species" species="puerensis">
<emphasis italics="true" pageId="0" pageNumber="51">N. puerensis</emphasis>
</taxonomicName>
colony surface is seen as white aerial mycelium, fluffy, with a serrate edge, and grayish to dark brown from center to edge. Therefore, based on morphological and phylogenetic analyses, we introduce
<taxonomicName lsidName="N. puerensis" pageId="0" pageNumber="51" rank="species" species="puerensis">
<emphasis italics="true" pageId="0" pageNumber="51">N. puerensis</emphasis>
</taxonomicName>
as a distinct new species.
</paragraph>
<caption doi="10.3897/mycokeys.94.95751.figure3" httpUri="https://binary.pensoft.net/fig/781090" pageId="0" pageNumber="51" start="Figure 3" startId="F3">
<paragraph pageId="0" pageNumber="51">
<emphasis bold="true" pageId="0" pageNumber="51">Figure 3.</emphasis>
<taxonomicName authorityName="L. Lu &amp; Tibpromma" authorityYear="2022" class="Dothideomycetes" family="Nigrogranaceae" genus="Nigrograna" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Nigrograna puerensis" order="Pleosporales" pageId="0" pageNumber="51" phylum="Ascomycota" rank="species" species="puerensis">
<emphasis italics="true" pageId="0" pageNumber="51">Nigrograna puerensis</emphasis>
</taxonomicName>
(ZHKU 22-0122, holotype)
<emphasis bold="true" pageId="0" pageNumber="51">a, b</emphasis>
ascomata observed on host substrate
<emphasis bold="true" pageId="0" pageNumber="51">c</emphasis>
a vertical section through an ascoma
<emphasis bold="true" pageId="0" pageNumber="51">d</emphasis>
peridium
<emphasis bold="true" pageId="0" pageNumber="51">e</emphasis>
hamathecium
<emphasis bold="true" pageId="0" pageNumber="51">f-j</emphasis>
asci
<emphasis bold="true" pageId="0" pageNumber="51">k</emphasis>
germinated ascospore
<emphasis bold="true" pageId="0" pageNumber="51">l-p</emphasis>
ascospores
<emphasis bold="true" pageId="0" pageNumber="51">q</emphasis>
culture on PDA from above and reverse. Scale bars: 50
<normalizedToken originalValue="μm">μm</normalizedToken>
(
<emphasis bold="true" pageId="0" pageNumber="51">c</emphasis>
); 30
<normalizedToken originalValue="μm">μm</normalizedToken>
(
<emphasis bold="true" pageId="0" pageNumber="51">d</emphasis>
); 15
<normalizedToken originalValue="μm">μm</normalizedToken>
(
<emphasis bold="true" pageId="0" pageNumber="51">e-k</emphasis>
); 5
<normalizedToken originalValue="μm">μm</normalizedToken>
(
<emphasis bold="true" pageId="0" pageNumber="51">l-p</emphasis>
).
</paragraph>
</caption>
</subSubSection>
</treatment>
</document>