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<mods:title id="4E1CC42ECAAD01ACBFFAECCD894DB656">Spirostomum teres: A Long Term Study of an Anoxic-Hypolimnion Population Feeding upon Photosynthesizing Microorganisms</mods:title>
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<taxonomicName id="E08715F2770DFFE3FCF5ACD993034EEA" ID-CoL="4Z8P3" authority="Claparède &amp; Lachmann, 1858" authorityName="Claparède &amp; Lachmann" authorityYear="1858" box="[816,1042,1418,1445]" class="Heterotrichea" family="Spirostomidae" genus="Spirostomum" kingdom="Chromista" order="Heterotrichida" pageId="4" pageNumber="17" phylum="Ciliophora" rank="species" species="teres">
<emphasis id="15F3B263770DFFE3FCF5ACD993034EEA" bold="true" box="[816,1042,1418,1445]" italics="true" pageId="4" pageNumber="17">Spirostomum teres</emphasis>
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The ciliate sampling dates and layers, and 
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<emphasis id="15F3B263770DFFE3FA9BACEF92A64E99" box="[1374,1463,1468,1494]" italics="true" pageId="4" pageNumber="17">S. teres</emphasis>
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numbers are pooled in the Figure (
<figureCitation id="BFBC72F4770DFFE3FB1CAC8C92334EB6" box="[1241,1314,1503,1529]" captionStart="Fig" captionStartId="6.[132,167,1102,1124]" captionTargetBox="[150,1460,234,1043]" captionTargetId="figure-6@6.[136,1466,219,1060]" captionTargetPageId="6" captionText="Fig. 3. a) Dissolved Oxygen isopleths, sampled data/depths (Analysed), and of Spirostomum teres numbers in Lake Alchichica; b) average S. teres numbers throughout an hypoxic/anoxic layer (based on Peštová et al. 2008, Bautista-Reyes and Macek 2012, Sánchez-Medina et al. 2016, and this study)." figureDoi="http://doi.org/10.5281/zenodo.10994481" httpUri="https://zenodo.org/record/10994481/files/figure.png" pageId="4" pageNumber="17">Fig. 3</figureCitation>
) along with the hypoxic layer (DO &lt;
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L 
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) average numbers of the ciliate. 
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<emphasis id="15F3B263770DFFE3FC7DAF7793014D71" box="[952,1040,1572,1598]" italics="true" pageId="4" pageNumber="17">S. teres</emphasis>
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was found first in the near bottom / 
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samples and in the oxycline from May through September/November since the launch of ciliate monitoring in Alchichica in 2003; the first years’ sampling was limited to five to 10 layers, i.e., the register would not be complete (
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<emphasis id="15F3B263770DFFE3FBC7AF8193484DA4" box="[1026,1113,1746,1772]" italics="true" pageId="4" pageNumber="17">S. teres</emphasis>
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was misidentified in 
<bibRefCitation id="43161380770DFFE3FA9EAF8194A84C41" author="Pestova D. &amp; Macek M. &amp; Martinez-Perez M. E." pageId="4" pageNumber="17" pagination="13 - 25" refId="ref14486" refString="Pestova D., Macek M., Martinez-Perez M. E. (2008) Ciliates and their picophytoplankton-feeding activity in a high altitude warm-monomictic saline lake. Europ. J. Protistol. 44: 13 - 25" type="journal article" year="2008">Peštová et al. 2008</bibRefCitation>
and 
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as 
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<emphasis id="15F3B263770DFFE3FCF5AE4494D04C7E" box="[816,961,1815,1841]" italics="true" pageId="4" pageNumber="17">Chaetospira</emphasis>
sp.
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). Typically, 
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<emphasis id="15F3B263770DFFE3FBBBAE4493C24C7E" box="[1150,1235,1815,1841]" italics="true" pageId="4" pageNumber="17">S. teres</emphasis>
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was colonizing the oxycline just after the stratification (May) but still it has been found in the very bottom layer until 2009 (the end of the first monitoring period). Maximum numbers of 700 cells L 
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were found within the sampled layers, while the average number integrated through the whole hypolimnion (
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&lt;1 mgL 
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) was found regularly up to 150 cells L 
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; a high number of 530 cells L 
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was found when the anoxic layer was limited to the very bottom layer on 
<date id="533948B1770FFFE1FF2EACE996774E9B" box="[235,358,1465,1492]" pageId="6" pageNumber="19" value="2005-05">May 2005</date>
.
</paragraph>
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<emphasis id="15F3B263770CFFE2FFB8AD9097C74F96" bold="true" box="[125,214,1219,1241]" pageId="5" pageNumber="18">Fig. 1. a)</emphasis>
Temperature and 
<emphasis id="15F3B263770CFFE2FE41AD9096884F96" bold="true" box="[388,409,1219,1241]" pageId="5" pageNumber="18">b)</emphasis>
photosynthetically active radiation profiles in Lake Alchichica.
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<emphasis id="15F3B263770CFFE2FFB8AE3397AF4C3A" bold="true" box="[125,190,1888,1910]" pageId="5" pageNumber="18">Fig. 2.</emphasis>
Heterotrophic (
<emphasis id="15F3B263770CFFE2FE9EAE33969A4C3A" bold="true" box="[347,395,1888,1909]" pageId="5" pageNumber="18">HPP</emphasis>
) and autotrophic picoplankton (
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) in Lake Alchichica (based on 
<bibRefCitation id="43161380770CFFE2FBEBAE3393FA4C3A" author="Pestova D. &amp; Macek M. &amp; Martinez-Perez M. E." box="[1070,1259,1888,1910]" pageId="5" pageNumber="18" pagination="13 - 25" refId="ref14486" refString="Pestova D., Macek M., Martinez-Perez M. E. (2008) Ciliates and their picophytoplankton-feeding activity in a high altitude warm-monomictic saline lake. Europ. J. Protistol. 44: 13 - 25" type="journal article" year="2008">Peštová et al. 2008</bibRefCitation>
, 
<bibRefCitation id="43161380770CFFE2FB32AE3392BA4C3A" author="Macek M. &amp; Alcocer J. &amp; Lugo-Vazquez A. &amp; Martinez-Perez M. E. &amp; Peralta-Soriano L. &amp; Vilaclara-Fatjo G." box="[1271,1451,1888,1910]" pageId="5" pageNumber="18" pagination="183 - 192" refId="ref14000" refString="Macek M., Alcocer J., Lugo-Vazquez A., Martinez-Perez M. E., Peralta-Soriano L., Vilaclara-Fatjo G. (2009) Long term pico- plankton dynamics in a warm-monomictic, tropical high altitude lake. J. Limnol. 68: 183 - 192" type="journal article" year="2009">Macek et al. 2009</bibRefCitation>
, 
<bibRefCitation id="43161380770CFFE2FFB8AE2F96874CDE" author="Hernandez-Aviles J. S. &amp; Macek M. &amp; Alcocer J. &amp; Lopez-Trejo B. &amp; Merino-Ibarra M." box="[125,406,1916,1938]" pageId="5" pageNumber="18" pagination="1301 - 1314" refId="ref13617" refString="Hernandez-Aviles J. S., Macek M., Alcocer J., Lopez-Trejo B., Merino-Ibarra M. (2010) Prokaryotic picoplankton dynamics in a warm-monomictic saline lake: temporal and spatial variation in structure and composition. J. Plankton Res. 32: 1301 - 1314" type="journal article" year="2010">Hernández-Avilés et al. 2010</bibRefCitation>
, 
<bibRefCitation id="43161380770CFFE2FE65AE2F95C84CDE" author="Bautista-Reyes F. &amp; Macek M." box="[416,729,1916,1938]" pageId="5" pageNumber="18" pagination="85 - 97" refId="ref12538" refString="Bautista-Reyes F., Macek M. (2012) Ciliate food vacuole content and bacterial community composition in the warm-monomictic crater lake Alchichica (Mexico). FEMS Microbiol. Ecol. 79: 85 - 97" type="journal article" year="2012">Bautista-Reyes and Macek 2012</bibRefCitation>
, 
<bibRefCitation id="43161380770CFFE2FD26AE2F94E04CDD" author="Sanchez-Medina X. &amp; Macek M. &amp; Bautista-Reyes F. &amp; Perz A. &amp; Chavez-Arteaga M." box="[739,1009,1916,1938]" pageId="5" pageNumber="18" pagination="179 - 190" refId="ref14628" refString="Sanchez-Medina X., Macek M., Bautista-Reyes F., Perz A., Bonil- la-Lemus P., Chavez-Arteaga M. (2016) Inter-annual ciliate distribution variation within the late stratification oxycline in a monomictic lake, Lake Alchichica (Mexico). J. Limnol. 75: 179 - 190" type="journal article" year="2016">Sánchez-Medina et al. 2016</bibRefCitation>
, 
<bibRefCitation id="43161380770CFFE2FC3EAE2F93BC4CDE" author="Pajares S. &amp; Merino-Ibarra M. &amp; Macek M. &amp; Alcocer J." box="[1019,1197,1916,1938]" pageId="5" pageNumber="18" pagination="1180 - 1193" refId="ref14443" refString="Pajares S., Merino-Ibarra M., Macek M., Alcocer J. (2017) Vertical and seasonal distribution of picoplankton and functional nitrogen genes in a high-altitude warm-monomictic tropical lake. Freshwater Biol. 62: 1180 - 1193" type="journal article" year="2017">Pájares et al. 2017</bibRefCitation>
, and this study).
</paragraph>
</caption>
<caption id="73F83EF9770FFFE1FF41AD1D96444FD3" ID-DOI="http://doi.org/10.5281/zenodo.10994481" ID-Zenodo-Dep="10994481" httpUri="https://zenodo.org/record/10994481/files/figure.png" pageId="6" pageNumber="19" startId="6.[132,167,1102,1124]" targetBox="[150,1460,234,1043]" targetPageId="6" targetType="figure">
<paragraph id="27386E71770FFFE1FF41AD1D96444FD3" blockId="6.[132,1467,1102,1180]" pageId="6" pageNumber="19">
<emphasis id="15F3B263770FFFE1FF41AD1D96864F2B" bold="true" box="[132,407,1102,1124]" pageId="6" pageNumber="19">Fig. 3. a) Dissolved Oxygen</emphasis>
isopleths, sampled data/depths (
<emphasis id="15F3B263770FFFE1FD08AD1D943D4F2C" bold="true" box="[717,812,1102,1123]" pageId="6" pageNumber="19">Analysed</emphasis>
), and of 
<taxonomicName id="E08715F2770FFFE1FC45AD1D93294F2C" box="[896,1080,1102,1123]" class="Heterotrichea" family="Spirostomidae" genus="Spirostomum" kingdom="Chromista" order="Heterotrichida" pageId="6" pageNumber="19" phylum="Ciliophora" rank="species" species="teres">
<emphasis id="15F3B263770FFFE1FC45AD1D93294F2C" bold="true" box="[896,1080,1102,1123]" italics="true" pageId="6" pageNumber="19">Spirostomum teres</emphasis>
</taxonomicName>
numbers in Lake Alchichica; 
<emphasis id="15F3B263770FFFE1FA90AD1D927B4F2B" bold="true" box="[1365,1386,1102,1124]" pageId="6" pageNumber="19">b)</emphasis>
average 
<taxonomicName id="E08715F2770FFFE1FF41AD3997DB4FCF" box="[132,202,1130,1152]" class="Heterotrichea" family="Spirostomidae" genus="Spirostomum" kingdom="Chromista" order="Heterotrichida" pageId="6" pageNumber="19" phylum="Ciliophora" rank="species" species="teres">
<emphasis id="15F3B263770FFFE1FF41AD3997DB4FCF" box="[132,202,1130,1152]" italics="true" pageId="6" pageNumber="19">S. teres</emphasis>
</taxonomicName>
numbers throughout an hypoxic/anoxic layer (based on 
<bibRefCitation id="43161380770FFFE1FD21AD39948B4F30" author="Pestova D. &amp; Macek M. &amp; Martinez-Perez M. E." box="[740,922,1130,1152]" pageId="6" pageNumber="19" pagination="13 - 25" refId="ref14486" refString="Pestova D., Macek M., Martinez-Perez M. E. (2008) Ciliates and their picophytoplankton-feeding activity in a high altitude warm-monomictic saline lake. Europ. J. Protistol. 44: 13 - 25" type="journal article" year="2008">Peštová et al. 2008</bibRefCitation>
, 
<bibRefCitation id="43161380770FFFE1FC61AD3993CD4F30" author="Bautista-Reyes F. &amp; Macek M." box="[932,1244,1130,1152]" pageId="6" pageNumber="19" pagination="85 - 97" refId="ref12538" refString="Bautista-Reyes F., Macek M. (2012) Ciliate food vacuole content and bacterial community composition in the warm-monomictic crater lake Alchichica (Mexico). FEMS Microbiol. Ecol. 79: 85 - 97" type="journal article" year="2012">Bautista-Reyes and Macek 2012</bibRefCitation>
, 
<bibRefCitation id="43161380770FFFE1FB20AD3997A74FD3" author="Sanchez-Medina X. &amp; Macek M. &amp; Bautista-Reyes F. &amp; Perz A. &amp; Chavez-Arteaga M." pageId="6" pageNumber="19" pagination="179 - 190" refId="ref14628" refString="Sanchez-Medina X., Macek M., Bautista-Reyes F., Perz A., Bonil- la-Lemus P., Chavez-Arteaga M. (2016) Inter-annual ciliate distribution variation within the late stratification oxycline in a monomictic lake, Lake Alchichica (Mexico). J. Limnol. 75: 179 - 190" type="journal article" year="2016">Sánchez-Medina et al. 2016</bibRefCitation>
, and this study).
</paragraph>
</caption>
<paragraph id="27386E71770FFFE1FF6FAC8F92874E9B" blockId="6.[132,779,1292,1941]" lastBlockId="6.[816,1463,1293,1943]" pageId="6" pageNumber="19">
Since 2011 when the sampling already included over 10 layers within- and below-oxycline (bubbles in the 
<figureCitation id="BFBC72F4770FFFE1FF75AF7297E74D74" box="[176,246,1569,1595]" captionStart="Fig" captionStartId="6.[132,167,1102,1124]" captionTargetBox="[150,1460,234,1043]" captionTargetId="figure-6@6.[136,1466,219,1060]" captionTargetPageId="6" captionText="Fig. 3. a) Dissolved Oxygen isopleths, sampled data/depths (Analysed), and of Spirostomum teres numbers in Lake Alchichica; b) average S. teres numbers throughout an hypoxic/anoxic layer (based on Peštová et al. 2008, Bautista-Reyes and Macek 2012, Sánchez-Medina et al. 2016, and this study)." figureDoi="http://doi.org/10.5281/zenodo.10994481" httpUri="https://zenodo.org/record/10994481/files/figure.png" pageId="6" pageNumber="19">Fig. 3</figureCitation>
), 
<taxonomicName id="E08715F2770FFFE1FECBAF7196754D74" box="[270,356,1570,1596]" class="Heterotrichea" family="Spirostomidae" genus="Spirostomum" kingdom="Chromista" order="Heterotrichida" pageId="6" pageNumber="19" phylum="Ciliophora" rank="species" species="teres">
<emphasis id="15F3B263770FFFE1FECBAF7196754D74" box="[270,356,1570,1596]" italics="true" pageId="6" pageNumber="19">S. teres</emphasis>
</taxonomicName>
has gradually disappeared from the bottom and it was found in quite restricted layer below the oxycline. However, still in 
<date id="533948B1770FFFE1FE32AF3595814DCF" box="[503,656,1638,1664]" pageId="6" pageNumber="19" value="2011-08">August 2011</date>
the maximum bottom abundance of 
<taxonomicName id="E08715F2770FFFE1FE00AFDA950B4DEC" box="[453,538,1673,1699]" class="Heterotrichea" family="Spirostomidae" genus="Spirostomum" kingdom="Chromista" order="Heterotrichida" pageId="6" pageNumber="19" phylum="Ciliophora" rank="species" species="teres">
<emphasis id="15F3B263770FFFE1FE00AFDA950B4DEC" box="[453,538,1673,1699]" italics="true" pageId="6" pageNumber="19">S. teres</emphasis>
</taxonomicName>
(1900 cells L 
<superScript id="D0F2C339770FFFE1FD7EAFDB95DD4DD9" attach="left" box="[699,716,1672,1686]" fontSize="6" pageId="6" pageNumber="19">–1</superScript>
) was observed. The samples were not taken during the whole stratification period but it seems the pelagic population of 
<taxonomicName id="E08715F2770FFFE1FF6CAFA296124C45" box="[169,259,1777,1803]" class="Heterotrichea" family="Spirostomidae" genus="Spirostomum" kingdom="Chromista" order="Heterotrichida" pageId="6" pageNumber="19" phylum="Ciliophora" rank="species" species="teres">
<emphasis id="15F3B263770FFFE1FF6CAFA296124C45" box="[169,259,1777,1803]" italics="true" pageId="6" pageNumber="19">S. teres</emphasis>
</taxonomicName>
was decreasing. Frequently, annual maximums of 1000 to 2100 cells L 
<superScript id="D0F2C339770FFFE1FE2FAE4196EA4C6F" attach="left" box="[490,507,1810,1824]" fontSize="6" pageId="6" pageNumber="19">–1</superScript>
were found during the stable stratification with the light penetrating the microaerobic layer (July–August of 2011, 2012, 2013, 2016). On the other hand, 
<taxonomicName id="E08715F2770FFFE1FEAAAE2896D74CDB" box="[367,454,1915,1941]" class="Heterotrichea" family="Spirostomidae" genus="Spirostomum" kingdom="Chromista" order="Heterotrichida" pageId="6" pageNumber="19" phylum="Ciliophora" rank="species" species="teres">
<emphasis id="15F3B263770FFFE1FEAAAE2896D74CDB" box="[367,454,1915,1941]" italics="true" pageId="6" pageNumber="19">S. teres</emphasis>
</taxonomicName>
has not been found during the finishing stratification that was not already supported by strong temperature gradient while oxygen stratification persisted; in 2016, the last samplings within the study, 
<taxonomicName id="E08715F2770FFFE1FC6EAC2693104EC1" box="[939,1025,1397,1423]" class="Heterotrichea" family="Spirostomidae" genus="Spirostomum" kingdom="Chromista" order="Heterotrichida" pageId="6" pageNumber="19" phylum="Ciliophora" rank="species" species="teres">
<emphasis id="15F3B263770FFFE1FC6EAC2693104EC1" box="[939,1025,1397,1423]" italics="true" pageId="6" pageNumber="19">S. teres</emphasis>
</taxonomicName>
was absent already from November. Additionally, January peak (265 cells L 
<superScript id="D0F2C339770FFFE1FAD6ACC592354EEB" attach="left" box="[1299,1316,1430,1444]" fontSize="6" pageId="6" pageNumber="19">–1</superScript>
) was found around 
<quantity id="E07FC394770FFFE1FC4CACEA94D44E9B" box="[905,965,1465,1492]" metricMagnitude="1" metricUnit="m" metricValue="4.5" pageId="6" pageNumber="19" unit="m" value="45.0">45 m</quantity>
during the uncomplete mixing in 2016.
</paragraph>
<paragraph id="27386E71770FFFE1FC93AC8F92614CD9" blockId="6.[816,1463,1293,1943]" pageId="6" pageNumber="19">
Examples of the whole limnologic variables, which accompanied the typical 
<taxonomicName id="E08715F2770FFFE1FBA1ACAC93AE4D57" box="[1124,1215,1535,1561]" class="Heterotrichea" family="Spirostomidae" genus="Spirostomum" kingdom="Chromista" order="Heterotrichida" pageId="6" pageNumber="19" phylum="Ciliophora" rank="species" species="teres">
<emphasis id="15F3B263770FFFE1FBA1ACAC93AE4D57" box="[1124,1215,1535,1561]" italics="true" pageId="6" pageNumber="19">S. teres</emphasis>
</taxonomicName>
vertical distribution are pooled in the 
<figureCitation id="BFBC72F4770FFFE1FBC0AF72935E4D74" box="[1029,1103,1569,1595]" captionStart="Fig" captionStartId="7.[125,160,1829,1851]" captionTargetBox="[153,1442,236,1744]" captionTargetId="figure-8@7.[125,1455,217,1759]" captionTargetPageId="7" captionText="Fig. 4. Representative early- (a to h) and late-stratification (i to p) of Spirostomum teres in Lake Alchichica. a, e, i, m) Temperature, T [°C], Dissolved oxygen, DO [mg L–1], and Photosynthetically active radiation PAR [%]; b, f, j, n) numbers of heterotrophic-, HPP [cells mL–1] and autotrophic picoplankton, APP [cells mL–1], and Spirostomum teres [cells L–1]; c, g, k, o) nitrites, NO –, nitrates NO – and ammonium, 2 3 NH [µmol L–1]; d, h, l, p) chlorophyll a, Chl a [µg L–1], phycoerythrin, PE [µg L–1], and phycocyanin, PC [ng L–1]." figureDoi="http://doi.org/10.5281/zenodo.10994483" httpUri="https://zenodo.org/record/10994483/files/figure.png" pageId="6" pageNumber="19">Fig. 4</figureCitation>
. In Alchichica, the ciliate distribution was extended through the metalimnion during July–August (
<figureCitation id="BFBC72F4770FFFE1FC16AF35935D4DCE" box="[979,1100,1638,1665]" captionStart="Fig" captionStartId="7.[125,160,1829,1851]" captionTargetBox="[153,1442,236,1744]" captionTargetId="figure-8@7.[125,1455,217,1759]" captionTargetPageId="7" captionText="Fig. 4. Representative early- (a to h) and late-stratification (i to p) of Spirostomum teres in Lake Alchichica. a, e, i, m) Temperature, T [°C], Dissolved oxygen, DO [mg L–1], and Photosynthetically active radiation PAR [%]; b, f, j, n) numbers of heterotrophic-, HPP [cells mL–1] and autotrophic picoplankton, APP [cells mL–1], and Spirostomum teres [cells L–1]; c, g, k, o) nitrites, NO –, nitrates NO – and ammonium, 2 3 NH [µmol L–1]; d, h, l, p) chlorophyll a, Chl a [µg L–1], phycoerythrin, PE [µg L–1], and phycocyanin, PC [ng L–1]." figureDoi="http://doi.org/10.5281/zenodo.10994483" httpUri="https://zenodo.org/record/10994483/files/figure.png" pageId="6" pageNumber="19">Fig. 4 a–h</figureCitation>
). There was no evident exceeding maximum but 
<taxonomicName id="E08715F2770FFFE1FBCCAFDA934E4DEC" box="[1033,1119,1673,1699]" class="Heterotrichea" family="Spirostomidae" genus="Spirostomum" kingdom="Chromista" order="Heterotrichida" pageId="6" pageNumber="19" phylum="Ciliophora" rank="species" species="teres">
<emphasis id="15F3B263770FFFE1FBCCAFDA934E4DEC" box="[1033,1119,1673,1699]" italics="true" pageId="6" pageNumber="19">S. teres</emphasis>
</taxonomicName>
was observed in much wider metalimnion-hypolimnion boundary layers, following cyanobacterial pigments distribution, below the chlorophyll 
<emphasis id="15F3B263770FFFE1FC4BAFA2948C4C45" box="[910,925,1777,1802]" italics="true" pageId="6" pageNumber="19">a</emphasis>
, phycoerythrin and phycocyanin maximums. Still, apparent photosynthetic anoxygenic bacteria (
<collectionCode id="4196F6B4770FFFE1FCFDAE6594634C1F" box="[824,882,1846,1872]" pageId="6" pageNumber="19">PAB</collectionCode>
) were not so important there. Maximum abundances were found located 
<quantity id="E07FC394770FFFE1FBB1AE0993C84C3B" box="[1140,1241,1882,1908]" metricMagnitude="0" metricUnit="m" metricValue="3.0" metricValueMax="4.0" metricValueMin="2.0" pageId="6" pageNumber="19" unit="m" value="3.0" valueMax="4.0" valueMin="2.0">2 to 4 m</quantity>
below the limit of DO. Also nitrates were sometimes peaking there.
</paragraph>
<caption id="73F83EF9770EFFE0FFB8AE7693F04CC0" ID-DOI="http://doi.org/10.5281/zenodo.10994483" ID-Zenodo-Dep="10994483" httpUri="https://zenodo.org/record/10994483/files/figure.png" pageId="7" pageNumber="20" startId="7.[125,160,1829,1851]" targetBox="[153,1442,236,1744]" targetPageId="7" targetType="figure">
<paragraph id="27386E71770EFFE0FFB8AE7693F04CC0" blockId="7.[125,1456,1829,1936]" pageId="7" pageNumber="20">
<emphasis id="15F3B263770EFFE0FFB8AE7697AD4C75" bold="true" box="[125,188,1829,1851]" pageId="7" pageNumber="20">Fig. 4.</emphasis>
Representative early- (
<emphasis id="15F3B263770EFFE0FE5FAE7596B74C74" bold="true" box="[410,422,1830,1851]" pageId="7" pageNumber="20">a</emphasis>
to 
<emphasis id="15F3B263770EFFE0FE01AE7596C04C74" bold="true" box="[452,465,1830,1851]" pageId="7" pageNumber="20">h</emphasis>
) and late-stratification (
<emphasis id="15F3B263770EFFE0FD73AE7695AC4C74" bold="true" box="[694,701,1829,1851]" pageId="7" pageNumber="20">i</emphasis>
to 
<emphasis id="15F3B263770EFFE0FD1EAE7595F94C74" bold="true" box="[731,744,1830,1851]" pageId="7" pageNumber="20">p</emphasis>
) of 
<taxonomicName id="E08715F2770EFFE0FCCAAE7594D14C74" box="[783,960,1830,1851]" class="Heterotrichea" family="Spirostomidae" genus="Spirostomum" kingdom="Chromista" order="Heterotrichida" pageId="7" pageNumber="20" phylum="Ciliophora" rank="species" species="teres">
<emphasis id="15F3B263770EFFE0FCCAAE7594D14C74" box="[783,960,1830,1851]" italics="true" pageId="7" pageNumber="20">Spirostomum teres</emphasis>
</taxonomicName>
in Lake Alchichica. 
<emphasis id="15F3B263770EFFE0FB41AE7593F14C74" bold="true" box="[1156,1248,1829,1851]" pageId="7" pageNumber="20">a, e, i, m)</emphasis>
Temperature, 
<emphasis id="15F3B263770EFFE0FAACAE7592B84C74" bold="true" box="[1385,1449,1829,1851]" pageId="7" pageNumber="20">T [°C]</emphasis>
, Dissolved oxygen, 
<emphasis id="15F3B263770EFFE0FEF0AE1296A84C18" bold="true" box="[309,441,1857,1879]" pageId="7" pageNumber="20">
DO [mg L 
<superScript id="D0F2C339770EFFE0FE58AE1296BA4C02" attach="left" box="[413,427,1857,1869]" fontSize="5" pageId="7" pageNumber="20">–1</superScript>
],
</emphasis>
and Photosynthetically active radiation 
<emphasis id="15F3B263770EFFE0FCFFAE1294894C18" bold="true" box="[826,920,1857,1879]" pageId="7" pageNumber="20">PAR [%]</emphasis>
; 
<emphasis id="15F3B263770EFFE0FC61AE1194EB4C18" bold="true" box="[932,1018,1857,1879]" pageId="7" pageNumber="20">b, f, j, n)</emphasis>
numbers of heterotrophic-, 
<emphasis id="15F3B263770EFFE0FAC2AE1192BE4C18" bold="true" box="[1287,1455,1857,1879]" pageId="7" pageNumber="20">
HPP [cells mL 
<superScript id="D0F2C339770EFFE0FA5CAE1292B64C02" attach="left" box="[1433,1447,1857,1869]" fontSize="5" pageId="7" pageNumber="20">–1</superScript>
]
</emphasis>
and autotrophic picoplankton, 
<emphasis id="15F3B263770EFFE0FE65AE0E95594C3C" bold="true" box="[416,584,1885,1907]" pageId="7" pageNumber="20">
APP [cells mL 
<superScript id="D0F2C339770EFFE0FDF7AE0E95514C26" attach="left" box="[562,576,1885,1897]" fontSize="5" pageId="7" pageNumber="20">–1</superScript>
]
</emphasis>
, and 
<emphasis id="15F3B263770EFFE0FDBEAE0E94894C3C" bold="true" box="[635,920,1885,1907]" pageId="7" pageNumber="20">
<taxonomicName id="E08715F2770EFFE0FDBEAE0E94254C3C" box="[635,820,1885,1907]" class="Heterotrichea" family="Spirostomidae" genus="Spirostomum" kingdom="Chromista" order="Heterotrichida" pageId="7" pageNumber="20" phylum="Ciliophora" rank="species" species="teres">
<emphasis id="15F3B263770EFFE0FDBEAE0E94254C3C" bold="true" box="[635,820,1885,1907]" italics="true" pageId="7" pageNumber="20">Spirostomum teres</emphasis>
</taxonomicName>
[cells L 
<superScript id="D0F2C339770EFFE0FC47AE0E94814C26" attach="left" box="[898,912,1885,1897]" fontSize="5" pageId="7" pageNumber="20">–1</superScript>
]
</emphasis>
; 
<emphasis id="15F3B263770EFFE0FC60AE0D94EE4C3C" bold="true" box="[933,1023,1885,1907]" pageId="7" pageNumber="20">c, g, k, o)</emphasis>
nitrites, 
<emphasis id="15F3B263770EFFE0FB96AE0E93944C26" bold="true" box="[1107,1157,1885,1907]" pageId="7" pageNumber="20">
NO 
<superScript id="D0F2C339770EFFE0FBBBAE0E93944C26" attach="right" box="[1150,1157,1885,1897]" fontSize="5" pageId="7" pageNumber="20">–</superScript>
</emphasis>
, nitrates 
<emphasis id="15F3B263770EFFE0FB19AE0E921F4C26" bold="true" box="[1244,1294,1885,1907]" pageId="7" pageNumber="20">
NO 
<superScript id="D0F2C339770EFFE0FAC2AE0E921F4C26" attach="right" box="[1287,1294,1885,1897]" fontSize="5" pageId="7" pageNumber="20">–</superScript>
</emphasis>
and ammonium, 
<emphasis id="15F3B263770EFFE0FBB2AE3E96074CC0" bold="true" pageId="7" pageNumber="20">
<subScript id="BB036C34770EFFE0FBB2AE3E92164C36" attach="left" box="[1143,1287,1901,1913]" fontSize="5" pageId="7" pageNumber="20">2 3</subScript>
NH [µmol L 
<superScript id="D0F2C339770EFFE0FEC5AE2A961F4CCA" attach="left" box="[256,270,1913,1925]" fontSize="5" pageId="7" pageNumber="20">–1</superScript>
]
</emphasis>
; 
<emphasis id="15F3B263770EFFE0FEE6AE29966C4CC0" bold="true" box="[291,381,1913,1935]" pageId="7" pageNumber="20">d, h, l, p)</emphasis>
chlorophyll 
<emphasis id="15F3B263770EFFE0FE3DAE2995154CC0" box="[504,516,1914,1935]" italics="true" pageId="7" pageNumber="20">a</emphasis>
, 
<emphasis id="15F3B263770EFFE0FDD5AE2A958A4CC0" bold="true" box="[528,667,1913,1935]" pageId="7" pageNumber="20">
Chl 
<emphasis id="15F3B263770EFFE0FDF9AE2995594CC0" bold="true" box="[572,584,1914,1935]" italics="true" pageId="7" pageNumber="20">a</emphasis>
[µg L 
<superScript id="D0F2C339770EFFE0FD40AE2A95824CCA" attach="left" box="[645,659,1913,1925]" fontSize="5" pageId="7" pageNumber="20">–1</superScript>
]
</emphasis>
, phycoerythrin, 
<emphasis id="15F3B263770EFFE0FCFFAE2994BD4CC0" bold="true" box="[826,940,1913,1935]" pageId="7" pageNumber="20">
PE [µg L 
<superScript id="D0F2C339770EFFE0FC53AE2A94B54CCA" attach="left" box="[918,932,1913,1925]" fontSize="5" pageId="7" pageNumber="20">–1</superScript>
]
</emphasis>
, and phycocyanin, 
<emphasis id="15F3B263770EFFE0FBADAE2A93CA4CC0" bold="true" box="[1128,1243,1913,1935]" pageId="7" pageNumber="20">
PC [ng L 
<superScript id="D0F2C339770EFFE0FB00AE2A93C24CCA" attach="left" box="[1221,1235,1913,1925]" fontSize="5" pageId="7" pageNumber="20">–1</superScript>
]
</emphasis>
.
</paragraph>
</caption>
<paragraph id="27386E71770EFFE0FF64AEDA97B94CDA" blockId="7.[125,1456,1829,1936]" box="[161,168,1929,1941]" pageId="7" pageNumber="20">
<subScript id="BB036C34770EFFE0FF64AEDA97B94CDA" attach="left" box="[161,168,1929,1941]" fontSize="5" pageId="7" pageNumber="20">3</subScript>
</paragraph>
<caption id="73F83EF97701FFEFFF41AE7E96954CD8" ID-DOI="http://doi.org/10.5281/zenodo.10994485" ID-Zenodo-Dep="10994485" httpUri="https://zenodo.org/record/10994485/files/figure.png" pageId="8" pageNumber="21" startId="8.[132,167,1837,1859]" targetBox="[266,1322,223,1806]" targetPageId="8" targetType="figure">
<paragraph id="27386E717701FFEFFF41AE7E96954CD8" blockId="8.[132,1463,1837,1943]" pageId="8" pageNumber="21">
<emphasis id="15F3B2637701FFEFFF41AE7E97D54C0D" bold="true" box="[132,196,1837,1859]" pageId="8" pageNumber="21">Fig. 5.</emphasis>
Representative 
<taxonomicName id="E08715F27701FFEFFE9AAE7D95004C0C" box="[351,529,1838,1859]" class="Heterotrichea" family="Spirostomidae" genus="Spirostomum" kingdom="Chromista" order="Heterotrichida" pageId="8" pageNumber="21" phylum="Ciliophora" rank="species" species="teres">
<emphasis id="15F3B2637701FFEFFE9AAE7D95004C0C" box="[351,529,1838,1859]" italics="true" pageId="8" pageNumber="21">Spirostomum teres</emphasis>
</taxonomicName>
sampling profiles in Lake La Preciosa (
<emphasis id="15F3B2637701FFEFFC54AE7E948C4C0D" bold="true" box="[913,925,1837,1858]" pageId="8" pageNumber="21">a</emphasis>
to 
<emphasis id="15F3B2637701FFEFFC79AE7E94D64C0D" bold="true" box="[956,967,1837,1858]" pageId="8" pageNumber="21">c</emphasis>
) and Lake de La Cruz (
<emphasis id="15F3B2637701FFEFFB69AE7E93A84C0D" bold="true" box="[1196,1209,1837,1858]" pageId="8" pageNumber="21">d</emphasis>
to 
<emphasis id="15F3B2637701FFEFFB1CAE7E93F04C0C" bold="true" box="[1241,1249,1837,1859]" pageId="8" pageNumber="21">f</emphasis>
). 
<emphasis id="15F3B2637701FFEFFB30AE7E92104C0D" bold="true" box="[1269,1281,1837,1858]" pageId="8" pageNumber="21">a</emphasis>
, 
<emphasis id="15F3B2637701FFEFFAC8AE7E920B4C0D" bold="true" box="[1293,1306,1837,1858]" pageId="8" pageNumber="21">d</emphasis>
, 
<emphasis id="15F3B2637701FFEFFAE3AE7E92904C0C" bold="true" box="[1318,1409,1837,1859]" pageId="8" pageNumber="21">g) T [°C]</emphasis>
, Dissolved oxygen, 
<emphasis id="15F3B2637701FFEFFED9AE1A96B04C11" bold="true" box="[284,417,1864,1887]" pageId="8" pageNumber="21">
DO [mg L 
<superScript id="D0F2C3397701FFEFFE40AE1B96824C1B" attach="left" box="[389,403,1864,1876]" fontSize="5" pageId="8" pageNumber="21">–1</superScript>
],
</emphasis>
and Photosynthetically active radiation 
<emphasis id="15F3B2637701FFEFFCE0AE1A94924C10" bold="true" box="[805,899,1865,1887]" pageId="8" pageNumber="21">PAR [%]</emphasis>
; 
<emphasis id="15F3B2637701FFEFFC55AE1A948C4C11" bold="true" box="[912,925,1865,1886]" pageId="8" pageNumber="21">b</emphasis>
, 
<emphasis id="15F3B2637701FFEFFC6FAE1A94A44C11" bold="true" box="[938,949,1865,1886]" pageId="8" pageNumber="21">e</emphasis>
, 
<emphasis id="15F3B2637701FFEFFC04AE1A94C74C10" bold="true" box="[961,982,1865,1887]" pageId="8" pageNumber="21">h)</emphasis>
numbers of 
<emphasis id="15F3B2637701FFEFFB95AE1A93E84C11" bold="true" box="[1104,1273,1864,1886]" pageId="8" pageNumber="21">
APP [cells mL 
<superScript id="D0F2C3397701FFEFFB26AE1B93E04C1B" attach="left" box="[1251,1265,1864,1876]" fontSize="5" pageId="8" pageNumber="21">–1</superScript>
]
</emphasis>
(not analysed in de La Cruz) and of 
<emphasis id="15F3B2637701FFEFFEE3AE3695594C35" bold="true" box="[294,584,1892,1914]" pageId="8" pageNumber="21">
<taxonomicName id="E08715F27701FFEFFEE3AE3696F14C35" box="[294,480,1893,1914]" class="Heterotrichea" family="Spirostomidae" genus="Spirostomum" kingdom="Chromista" order="Heterotrichida" pageId="8" pageNumber="21" phylum="Ciliophora" rank="species" species="teres">
<emphasis id="15F3B2637701FFEFFEE3AE3696F14C35" bold="true" box="[294,480,1893,1914]" italics="true" pageId="8" pageNumber="21">Spirostomum teres</emphasis>
</taxonomicName>
[cells L 
<superScript id="D0F2C3397701FFEFFDF7AE3795514C3F" attach="left" box="[562,576,1892,1904]" fontSize="5" pageId="8" pageNumber="21">–1</superScript>
]
</emphasis>
; 
<emphasis id="15F3B2637701FFEFFD90AE3694054C35" bold="true" box="[597,788,1893,1915]" pageId="8" pageNumber="21">c) turbidity [NTU]</emphasis>
; 
<emphasis id="15F3B2637701FFEFFCE7AE3694594C34" bold="true" box="[802,840,1893,1915]" pageId="8" pageNumber="21">f, I)</emphasis>
chlorophyll 
<emphasis id="15F3B2637701FFEFFC00AE3594C64C34" box="[965,983,1894,1915]" italics="true" pageId="8" pageNumber="21">a,</emphasis>
<emphasis id="15F3B2637701FFEFFC1BAE36937D4C35" bold="true" box="[990,1132,1892,1915]" pageId="8" pageNumber="21">
Chl 
<emphasis id="15F3B2637701FFEFFBCFAE3693074C35" bold="true" box="[1034,1046,1893,1914]" italics="true" pageId="8" pageNumber="21">a</emphasis>
[µg L 
<superScript id="D0F2C3397701FFEFFB93AE3793754C3F" attach="left" box="[1110,1124,1892,1904]" fontSize="5" pageId="8" pageNumber="21">–1</superScript>
]
</emphasis>
, phycoerythrin, 
<emphasis id="15F3B2637701FFEFFAC9AE3692914C35" bold="true" box="[1292,1408,1892,1914]" pageId="8" pageNumber="21">
PE [µg L 
<superScript id="D0F2C3397701FFEFFAAFAE3792694C3F" attach="left" box="[1386,1400,1892,1904]" fontSize="5" pageId="8" pageNumber="21">–1</superScript>
]
</emphasis>
, and phycocyanin, 
<emphasis id="15F3B2637701FFEFFECEAED2966F4CD9" bold="true" box="[267,382,1920,1943]" pageId="8" pageNumber="21">
PC [ng L 
<superScript id="D0F2C3397701FFEFFEADAED396674CC3" attach="left" box="[360,374,1920,1932]" fontSize="5" pageId="8" pageNumber="21">–1</superScript>
]
</emphasis>
.
</paragraph>
</caption>
<paragraph id="27386E717700FFEEFF66A98C96B34AE8" blockId="9.[125,771,223,945]" pageId="9" pageNumber="22">
Contrasting situation was found at the end of the stratification period, when higher ciliate numbers were observed in a strictly limited oxygen boundary (
<figureCitation id="BFBC72F47700FFEEFD42A87695C94A70" box="[647,728,293,319]" captionStart="Fig" captionStartId="6.[132,167,1102,1124]" captionTargetBox="[150,1460,234,1043]" captionTargetId="figure-6@6.[136,1466,219,1060]" captionTargetPageId="6" captionText="Fig. 3. a) Dissolved Oxygen isopleths, sampled data/depths (Analysed), and of Spirostomum teres numbers in Lake Alchichica; b) average S. teres numbers throughout an hypoxic/anoxic layer (based on Peštová et al. 2008, Bautista-Reyes and Macek 2012, Sánchez-Medina et al. 2016, and this study)." figureDoi="http://doi.org/10.5281/zenodo.10994481" httpUri="https://zenodo.org/record/10994481/files/figure.png" pageId="9" pageNumber="22">Figs. 3</figureCitation>
, 
<figureCitation id="BFBC72F47700FFEEFD26A87695E54A70" box="[739,756,293,319]" captionStart="Fig" captionStartId="7.[125,160,1829,1851]" captionTargetBox="[153,1442,236,1744]" captionTargetId="figure-8@7.[125,1455,217,1759]" captionTargetPageId="7" captionText="Fig. 4. Representative early- (a to h) and late-stratification (i to p) of Spirostomum teres in Lake Alchichica. a, e, i, m) Temperature, T [°C], Dissolved oxygen, DO [mg L–1], and Photosynthetically active radiation PAR [%]; b, f, j, n) numbers of heterotrophic-, HPP [cells mL–1] and autotrophic picoplankton, APP [cells mL–1], and Spirostomum teres [cells L–1]; c, g, k, o) nitrites, NO –, nitrates NO – and ammonium, 2 3 NH [µmol L–1]; d, h, l, p) chlorophyll a, Chl a [µg L–1], phycoerythrin, PE [µg L–1], and phycocyanin, PC [ng L–1]." figureDoi="http://doi.org/10.5281/zenodo.10994483" httpUri="https://zenodo.org/record/10994483/files/figure.png" pageId="9" pageNumber="22">4</figureCitation>
), accompanied by above mentioned bacteria. Nitrite peak was not as high as in July but, percentually, it was important (
<figureCitation id="BFBC72F47700FFEEFF25A8DE96834AE7" box="[224,402,397,424]" captionStart="Fig" captionStartId="7.[125,160,1829,1851]" captionTargetBox="[153,1442,236,1744]" captionTargetId="figure-8@7.[125,1455,217,1759]" captionTargetPageId="7" captionText="Fig. 4. Representative early- (a to h) and late-stratification (i to p) of Spirostomum teres in Lake Alchichica. a, e, i, m) Temperature, T [°C], Dissolved oxygen, DO [mg L–1], and Photosynthetically active radiation PAR [%]; b, f, j, n) numbers of heterotrophic-, HPP [cells mL–1] and autotrophic picoplankton, APP [cells mL–1], and Spirostomum teres [cells L–1]; c, g, k, o) nitrites, NO –, nitrates NO – and ammonium, 2 3 NH [µmol L–1]; d, h, l, p) chlorophyll a, Chl a [µg L–1], phycoerythrin, PE [µg L–1], and phycocyanin, PC [ng L–1]." figureDoi="http://doi.org/10.5281/zenodo.10994483" httpUri="https://zenodo.org/record/10994483/files/figure.png" pageId="9" pageNumber="22">Fig. 4 c, g, k, o</figureCitation>
).
</paragraph>
<paragraph id="27386E717700FFEEFF66A8E395294937" blockId="9.[125,771,223,945]" pageId="9" pageNumber="22">
Very similar 
<taxonomicName id="E08715F27700FFEEFEF8A8E396854A85" box="[317,404,432,458]" class="Heterotrichea" family="Spirostomidae" genus="Spirostomum" kingdom="Chromista" order="Heterotrichida" pageId="9" pageNumber="22" phylum="Ciliophora" rank="species" species="teres">
<emphasis id="15F3B2637700FFEEFEF8A8E396854A85" box="[317,404,432,458]" italics="true" pageId="9" pageNumber="22">S. teres</emphasis>
</taxonomicName>
distribution pattern was found in nearby lake La Preciosa (
<figureCitation id="BFBC72F47700FFEEFE1CA88095494AA1" box="[473,600,466,494]" captionStart="Fig" captionStartId="8.[132,167,1837,1859]" captionTargetBox="[266,1322,223,1806]" captionTargetId="figure-7@8.[249,1337,204,1821]" captionTargetPageId="8" captionText="Fig. 5. Representative Spirostomum teres sampling profiles in Lake La Preciosa (a to c) and Lake de La Cruz (d to f). a, d, g) T [°C], Dissolved oxygen, DO [mg L–1], and Photosynthetically active radiation PAR [%]; b, e, h) numbers of APP [cells mL–1] (not analysed in de La Cruz) and of Spirostomum teres [cells L–1]; c) turbidity [NTU]; f, I) chlorophyll a, Chl a [µg L–1], phycoerythrin, PE [µg L–1], and phycocyanin, PC [ng L–1]." figureDoi="http://doi.org/10.5281/zenodo.10994485" httpUri="https://zenodo.org/record/10994485/files/figure.png" pageId="9" pageNumber="22">Fig. 5 a–f</figureCitation>
). 
<taxonomicName id="E08715F27700FFEEFDB5A88095DB4AA3" box="[624,714,467,493]" class="Heterotrichea" family="Spirostomidae" genus="Spirostomum" kingdom="Chromista" order="Heterotrichida" pageId="9" pageNumber="22" phylum="Ciliophora" rank="species" species="teres">
<emphasis id="15F3B2637700FFEEFDB5A88095DB4AA3" box="[624,714,467,493]" italics="true" pageId="9" pageNumber="22">S. teres</emphasis>
</taxonomicName>
was found in higher numbers in similar DO conditions but below the phycocyanin maximum (composed by picocyanobacteria colonies and/or aggregates) and just above the maximum of chlorophyll 
<emphasis id="15F3B2637700FFEEFDE7AB0C95294937" box="[546,568,607,632]" italics="true" pageId="9" pageNumber="22">a.</emphasis>
</paragraph>
<paragraph id="27386E717700FFEEFF66ABD296844869" blockId="9.[125,771,223,945]" pageId="9" pageNumber="22">
A meromictic Lake La Cruz (
<collectingCountry id="5F902EE17700FFEEFE3EABD3955049D4" box="[507,577,640,667]" name="Spain" pageId="9" pageNumber="22">Spain</collectingCountry>
) showed similar 
<taxonomicName id="E08715F27700FFEEFFB8ABF797C249F2" box="[125,211,676,702]" class="Heterotrichea" family="Spirostomidae" genus="Spirostomum" kingdom="Chromista" order="Heterotrichida" pageId="9" pageNumber="22" phylum="Ciliophora" rank="species" species="teres">
<emphasis id="15F3B2637700FFEEFFB8ABF797C249F2" box="[125,211,676,702]" italics="true" pageId="9" pageNumber="22">S. teres</emphasis>
</taxonomicName>
distribution pattern even though on a ¼ m distance basis (
<figureCitation id="BFBC72F47700FFEEFECFAB95967549AF" box="[266,356,710,737]" captionStart="Fig" captionStartId="8.[132,167,1837,1859]" captionTargetBox="[266,1322,223,1806]" captionTargetId="figure-7@8.[249,1337,204,1821]" captionTargetPageId="8" captionText="Fig. 5. Representative Spirostomum teres sampling profiles in Lake La Preciosa (a to c) and Lake de La Cruz (d to f). a, d, g) T [°C], Dissolved oxygen, DO [mg L–1], and Photosynthetically active radiation PAR [%]; b, e, h) numbers of APP [cells mL–1] (not analysed in de La Cruz) and of Spirostomum teres [cells L–1]; c) turbidity [NTU]; f, I) chlorophyll a, Chl a [µg L–1], phycoerythrin, PE [µg L–1], and phycocyanin, PC [ng L–1]." figureDoi="http://doi.org/10.5281/zenodo.10994485" httpUri="https://zenodo.org/record/10994485/files/figure.png" pageId="9" pageNumber="22">Fig. 5 d</figureCitation>
to f). The ciliate was found peaking in a very narrow layer just within the maximum of pigments, in a limit of DO.
</paragraph>
<paragraph id="27386E717700FFEEFF66AA7D957E48FE" blockId="9.[125,771,223,945]" pageId="9" pageNumber="22">
According to QPS preparations, the ciliates from the three lakes were morphologically very similar even though, different composition of water did not permit to obtain all cells equal impregnated (
<figureCitation id="BFBC72F47700FFEEFDDCAAC5957048FE" box="[537,609,918,945]" captionStart="Fig" captionStartId="10.[136,171,1880,1902]" captionTargetBox="[253,1330,223,1861]" captionTargetId="figure-7@10.[253,1330,223,1861]" captionTargetPageId="10" captionText="Fig. 6. Spirostomum teres: a) Lake Alchichica (8/8/2011, 30 m), b) Lake La Preciosa (2/9/2011, 20.5 m and c) Lake de La Cruz (25/6/2010, 11.25 m. Protargol stain (QPS). 1 ≡ 10 µm." figureDoi="http://doi.org/10.5281/zenodo.10994487" httpUri="https://zenodo.org/record/10994487/files/figure.png" pageId="9" pageNumber="22">Fig. 6</figureCitation>
).
</paragraph>
</subSubSection>
<subSubSection id="6F9D3DFA7700FFE8FFB8AA9B94804AA2" lastPageId="15" lastPageNumber="28" pageId="9" pageNumber="22" type="biology_ecology">
<paragraph id="27386E717700FFEEFFB8AA9B96D048AD" blockId="9.[125,449,968,995]" box="[125,449,968,995]" pageId="9" pageNumber="22">
<heading id="7C70D91D7700FFEEFFB8AA9B96D048AD" box="[125,449,968,995]" fontSize="11" level="2" pageId="9" pageNumber="22" reason="5">
<emphasis id="15F3B2637700FFEEFFB8AA9B96D048AD" bold="true" box="[125,449,968,995]" pageId="9" pageNumber="22">
<taxonomicName id="E08715F27700FFEEFFB8AA9B964F48AC" ID-CoL="4Z8P3" box="[125,350,968,995]" class="Heterotrichea" family="Spirostomidae" genus="Spirostomum" kingdom="Chromista" order="Heterotrichida" pageId="9" pageNumber="22" phylum="Ciliophora" rank="species" species="teres">
<emphasis id="15F3B2637700FFEEFFB8AA9B964F48AC" bold="true" box="[125,350,968,995]" italics="true" pageId="9" pageNumber="22">Spirostomum teres</emphasis>
</taxonomicName>
feeding
</emphasis>
</heading>
</paragraph>
<paragraph id="27386E717700FFEEFF66AAA9954C4EB4" blockId="9.[125,771,1018,1947]" pageId="9" pageNumber="22">
During the study, the samples inspected 
<emphasis id="15F3B2637700FFEEFD41AAA895C44F5B" box="[644,725,1019,1044]" italics="true" pageId="9" pageNumber="22">in vivo</emphasis>
did not present any colour that could be related directly to their food (the ciliate was yellowish to brown due to its pigment granules). Vacuole content was analysed in all 
<taxonomicName id="E08715F27700FFEEFFB8ADD597C04FD0" box="[125,209,1158,1184]" class="Heterotrichea" family="Spirostomidae" genus="Spirostomum" kingdom="Chromista" order="Heterotrichida" pageId="9" pageNumber="22" phylum="Ciliophora" rank="species" species="teres">
<emphasis id="15F3B2637700FFEEFFB8ADD597C04FD0" box="[125,209,1158,1184]" italics="true" pageId="9" pageNumber="22">S. teres</emphasis>
</taxonomicName>
samples using its fluorescence &amp; 
<collectionCode id="4196F6B47700FFEEFD9EADD5958E4FEF" box="[603,671,1158,1184]" pageId="9" pageNumber="22">DAPI</collectionCode>
staining both in the quantitative ciliate samples fixed primarily with Lugol and in the samples fixed with formalin, in which fluorescence of picocyanobacteria and chlorophyll bearing eukaryotes persisted longer – up to one week; FLB were very well distinguishable in both treatments. However, to observe 
<collectionCode id="4196F6B47700FFEEFE09AC0595014E3F" box="[460,528,1366,1392]" pageId="9" pageNumber="22">DAPI</collectionCode>
stained heterotrophic bacteria inside the vacuoles was almost impossible for the samples fixed with Lugol but according to the size of a vacuole and the content of 
<collectionCode id="4196F6B47700FFEEFDECACEC954F4E96" box="[553,606,1471,1497]" country="Italy" httpUri="http://biocol.org/urn:lsid:biocol.org:col:13085" lsid="urn:lsid:biocol.org:col:13085" name="Parco Nazionale del Gran Sasso e Monti della Laga - Università di Camerino" pageId="9" pageNumber="22" type="Herbarium">APP</collectionCode>
we were able to estimate their relative content (
<figureCitation id="BFBC72F47700FFEEFDC2ACB2955C4EB3" box="[519,589,1505,1532]" captionStart="Fig" captionStartId="11.[125,160,1892,1914]" captionTargetBox="[225,1346,223,1859]" captionTargetId="figure-39@11.[225,1346,223,1859]" captionTargetPageId="11" captionText="Fig. 7. Spirostomum teres in Alchichica on July 2016 (epifluorescence microscope, Leica DMLB). a) DAPI staining; b) Phycobilin filter set (Y3); c) Chlorophyll a filter set (I3). 1 ≡ 10 µm." figureDoi="http://doi.org/10.5281/zenodo.10994489" httpUri="https://zenodo.org/record/10994489/files/figure.png" pageId="9" pageNumber="22">Fig. 7</figureCitation>
).
</paragraph>
<paragraph id="27386E717700FFEEFF66AF57950D4CD4" blockId="9.[125,771,1018,1947]" pageId="9" pageNumber="22">
Samples from all three lakes showed abundant 
<collectionCode id="4196F6B47700FFEEFD0BAF5794124D51" box="[718,771,1540,1566]" country="Italy" httpUri="http://biocol.org/urn:lsid:biocol.org:col:13085" lsid="urn:lsid:biocol.org:col:13085" name="Parco Nazionale del Gran Sasso e Monti della Laga - Università di Camerino" pageId="9" pageNumber="22" type="Herbarium">APP</collectionCode>
inside the vacuoles along with algae (
<figureCitation id="BFBC72F47700FFEEFDF2AF74956F4D0E" box="[567,638,1575,1601]" captionStart="Fig" captionStartId="12.[132,167,843,865]" captionTargetBox="[132,1462,223,815]" captionTargetId="figure-6@12.[132,1462,223,815]" captionTargetPageId="12" captionText="Fig. 8. Spirostomum teres inAlchichica on July 2013 (epifluorescence microscope, Leica DMLB). a) DAPI filter set (A); b) Phycobilin filter set (Y3); c) Chlorophyll a filter set (I3). 1 ≡ 10 µm." figureDoi="http://doi.org/10.5281/zenodo.10994491" httpUri="https://zenodo.org/record/10994491/files/figure.png" pageId="9" pageNumber="22">Fig. 8</figureCitation>
). Alchichica samples presented relative lower amount of then but still, they were observed throughout the season in all layers, including the very bottom. In Alchichica, within the larger feeding particles, apparent purple sulphur bacteria (
<taxonomicName id="E08715F27700FFEEFF28AF8796764DA1" authorityName="Winogradsky" authorityYear="1888" box="[237,359,1748,1774]" class="Gammaproteobacteria" family="Chromatiaceae" genus="Thiocapsa" kingdom="Bacteria" order="Chromatiales" pageId="9" pageNumber="22" phylum="Proteobacteria" rank="genus">
<emphasis id="15F3B2637700FFEEFF28AF8796764DA1" box="[237,359,1748,1774]" italics="true" pageId="9" pageNumber="22">Thiocapsa</emphasis>
</taxonomicName>
-like; genus to be confirmed) were observed in protargol impregnated samples and in IRtaken autofluorescence images (
<figureCitation id="BFBC72F47700FFEEFE30AE49952C4C7B" box="[501,573,1818,1844]" captionStart="Fig" captionStartId="12.[132,167,1578,1600]" captionTargetBox="[132,1462,960,1552]" captionTargetId="figure-7@12.[132,1462,960,1552]" captionTargetPageId="12" captionText="Fig. 9. Lake Alchichica Spirostomum teres with apparent purple sulphur bacteria in Protargol stain a) 4/12/2012, 36.5 m; b) 19/11/14, 38 m, and in infrared autofluorescence b) 19/11/14, 38 m. 1 ≡ 10 µm." figureDoi="http://doi.org/10.5281/zenodo.10994493" httpUri="https://zenodo.org/record/10994493/files/figure.png" pageId="9" pageNumber="22">Fig. 9</figureCitation>
), typically at the end of stratification (October–November). However, throughout the study, sulphur bacteria did not form the dominant content of food vacuoles.
</paragraph>
<paragraph id="27386E717700FFEEFC8AA98C94C54AEC" blockId="9.[809,1456,223,1404]" pageId="9" pageNumber="22">Sulphur bacteria were not found abundant inside the cells neither in La Preciosa or La Cruz samples, even though they were very abundant in the latter lake. Other apparently photosynthetic anoxygenic bacteria from La Preciosa (numbers not shown) were not observed ever to be ingested.</paragraph>
<paragraph id="27386E717700FFEEFC8AA8F892A14813" blockId="9.[809,1456,223,1404]" pageId="9" pageNumber="22">
Other photosynthetic organisms like green algae or diatoms were found frequently although, with some exception, forming only a part of the cell content. Alga 
<taxonomicName id="E08715F27700FFEEFCAAAB4293B04964" box="[879,1185,529,555]" class="Chlorophyceae" family="Selenastraceae" genus="Monoraphidium" kingdom="Plantae" order="Sphaeropleales" pageId="9" pageNumber="22" phylum="Chlorophyta" rank="species" species="minutum">
<emphasis id="15F3B2637700FFEEFCAAAB4293B04964" box="[879,1185,529,555]" italics="true" pageId="9" pageNumber="22">Monoraphidium minutum</emphasis>
</taxonomicName>
, which was observed frequently throughout the water column even during stratification (including hypoxic/anoxic hypolimnion), was the most important ingested eukaryote but only in the 
<date id="533948B17700FFEEFC96ABCB94D949FD" box="[851,968,664,690]" pageId="9" pageNumber="22" value="2016-07">July 2016</date>
, several specimens hosted dominantly the alga (
<figureCitation id="BFBC72F47700FFEEFCAEABE994D3499A" box="[875,962,698,725]" captionStart="Fig" captionStartId="11.[125,160,1892,1914]" captionTargetBox="[225,1346,223,1859]" captionTargetId="figure-39@11.[225,1346,223,1859]" captionTargetPageId="11" captionText="Fig. 7. Spirostomum teres in Alchichica on July 2016 (epifluorescence microscope, Leica DMLB). a) DAPI staining; b) Phycobilin filter set (Y3); c) Chlorophyll a filter set (I3). 1 ≡ 10 µm." figureDoi="http://doi.org/10.5281/zenodo.10994489" httpUri="https://zenodo.org/record/10994489/files/figure.png" pageId="9" pageNumber="22">Fig. 7c</figureCitation>
). Other typical food was a minute diatom 
<taxonomicName id="E08715F27700FFEEFCECAB8F938149B9" authorityName="Prasad" authorityYear="1990" box="[809,1168,732,758]" class="Bacillariophyceae" family="Stephanodiscaceae" genus="Cyclotella" higherTaxonomySource="GBIF" kingdom="Chromista" order="Thalassiosirales" pageId="9" pageNumber="22" phylum="Ochrophyta" rank="species" species="choctawhatcheeana">
<emphasis id="15F3B2637700FFEEFCECAB8F938149B9" box="[809,1168,732,758]" italics="true" pageId="9" pageNumber="22">Cyclotella choctawhatcheeana</emphasis>
</taxonomicName>
, sometimes found in Alchichica 
<taxonomicName id="E08715F27700FFEEFC57ABAC94F94857" box="[914,1000,767,793]" class="Heterotrichea" family="Spirostomidae" genus="Spirostomum" kingdom="Chromista" order="Heterotrichida" pageId="9" pageNumber="22" phylum="Ciliophora" rank="species" species="teres">
<emphasis id="15F3B2637700FFEEFC57ABAC94F94857" box="[914,1000,767,793]" italics="true" pageId="9" pageNumber="22">S. teres</emphasis>
</taxonomicName>
while very numerous in La Cruz sampling and experiment (
<figureCitation id="BFBC72F47700FFEEFBEEAA73936E4874" box="[1067,1151,800,827]" captionStart="Fig" captionStartId="10.[136,171,1880,1902]" captionTargetBox="[253,1330,223,1861]" captionTargetId="figure-7@10.[253,1330,223,1861]" captionTargetPageId="10" captionText="Fig. 6. Spirostomum teres: a) Lake Alchichica (8/8/2011, 30 m), b) Lake La Preciosa (2/9/2011, 20.5 m and c) Lake de La Cruz (25/6/2010, 11.25 m. Protargol stain (QPS). 1 ≡ 10 µm." figureDoi="http://doi.org/10.5281/zenodo.10994487" httpUri="https://zenodo.org/record/10994487/files/figure.png" pageId="9" pageNumber="22">Fig. 6c</figureCitation>
). No important eukaryotes were found in the 
<taxonomicName id="E08715F27700FFEEFBC9AA1093744813" box="[1036,1125,835,861]" class="Heterotrichea" family="Spirostomidae" genus="Spirostomum" kingdom="Chromista" order="Heterotrichida" pageId="9" pageNumber="22" phylum="Ciliophora" rank="species" species="teres">
<emphasis id="15F3B2637700FFEEFBC9AA1093744813" box="[1036,1125,835,861]" italics="true" pageId="9" pageNumber="22">S. teres</emphasis>
</taxonomicName>
population of La Preciosa.
</paragraph>
<paragraph id="27386E717700FFEEFC8AAA3792A14FC1" blockId="9.[809,1456,223,1404]" pageId="9" pageNumber="22">
Feeding rate experiments (pooled in the 
<tableCitation id="6A055BCA7700FFEEFA8EAA3792B64831" box="[1355,1447,868,894]" captionStart="Table 1" captionStartId="13.[125,181,1890,1911]" captionTargetBox="[185,1396,262,1807]" captionTargetPageId="13" captionText="Table 1. Feeding experiments with S. teres (APP = autotrophic picoplankton [cells mL–1]: N = number of inspected ciliate cells; F = clearance rate [nL cell–1h–1]; G = grazing rate [cells cell–1h–1]; average was calculated as weighted by analysed ciliate cells in different layers)." httpUri="http://table.plazi.org/id/73F83EF97704FFEAFFB8AE31928A4CDB" pageId="9" pageNumber="22" tableUuid="73F83EF97704FFEAFFB8AE31928A4CDB">Table 1</tableCitation>
; 
<figureCitation id="BFBC72F47700FFEEFCECAAD5946F48EF" box="[809,894,902,928]" captionStart="Fig" captionStartId="14.[132,167,1576,1598]" captionTargetBox="[165,1415,245,1540]" captionTargetId="figure-7@14.[136,1466,221,1572]" captionTargetPageId="14" captionText="Fig. 10. Feeding experiments with FLB in Lake Alchichica (a, b), La Preciosa (c, d) and Lake de la Cruz (e, f). Triple DAPI/FITC/CY3 set (a, b); DAPI set (c, e), Chlorophyll a / FITC set. Arrow: Just filled vacuole. 1 ≡ 10 µm." figureDoi="http://doi.org/10.5281/zenodo.10994495" httpUri="https://zenodo.org/record/10994495/files/figure.png" pageId="9" pageNumber="22">Fig. 10</figureCitation>
) were evaluated during late stratification of the crater lakes giving similar results for the samples with abundant ciliates. Typical Alchichica clearance rate laid about 2000 nL cell 
<superScript id="D0F2C3397700FFEEFBC8AAB8930F48B6" attach="both" box="[1037,1054,1003,1017]" fontSize="6" pageId="9" pageNumber="22">–1</superScript>
h 
<superScript id="D0F2C3397700FFEEFBE8AAB8932F48B6" attach="left" box="[1069,1086,1003,1017]" fontSize="6" pageId="9" pageNumber="22">–1</superScript>
, which represented in average 130 to 210 cells cell 
<superScript id="D0F2C3397700FFEEFBDDAD5E93384F54" attach="both" box="[1048,1065,1037,1051]" fontSize="6" pageId="9" pageNumber="22">–1</superScript>
h 
<superScript id="D0F2C3397700FFEEFBFDAD5E93584F54" attach="left" box="[1080,1097,1037,1051]" fontSize="6" pageId="9" pageNumber="22">–1</superScript>
ingested. La Preciosa population showed roughly similar clearances but the grazing rates were much more variable in different layers reaching sampling averages 46 to 488 cells cell 
<superScript id="D0F2C3397700FFEEFACAAD2092314FCE" attach="right" box="[1295,1312,1139,1153]" fontSize="6" pageId="9" pageNumber="22">–1</superScript>
h 
<superScript id="D0F2C3397700FFEEFAEBAD20922E4FCE" attach="left" box="[1326,1343,1139,1153]" fontSize="6" pageId="9" pageNumber="22">–1</superScript>
ingested.
</paragraph>
<paragraph id="27386E717700FFEEFC8AADC593AE4E34" blockId="9.[809,1456,223,1404]" pageId="9" pageNumber="22">
Field measurement of Lake La Cruz 
<taxonomicName id="E08715F27700FFEEFACFADC592704FE0" box="[1290,1377,1174,1200]" class="Heterotrichea" family="Spirostomidae" genus="Spirostomum" kingdom="Chromista" order="Heterotrichida" pageId="9" pageNumber="22" phylum="Ciliophora" rank="species" species="teres">
<emphasis id="15F3B2637700FFEEFACFADC592704FE0" box="[1290,1377,1174,1200]" italics="true" pageId="9" pageNumber="22">S. teres</emphasis>
</taxonomicName>
population did not give positive results due to the logistic problems. However, the experiment with the whole community transported to the laboratory had success. The clearance rates were observed being much lower than those from Mexican lakes; on the other hand, grazing was similar to Alchichica data.
</paragraph>
<paragraph id="27386E717700FFEEFCECACC193434EE3" blockId="9.[809,1106,1426,1452]" box="[809,1106,1426,1452]" pageId="9" pageNumber="22">
<heading id="7C70D91D7700FFEEFCECACC193434EE3" box="[809,1106,1426,1452]" fontSize="11" level="2" pageId="9" pageNumber="22" reason="5">
<emphasis id="15F3B2637700FFEEFCECACC193434EE3" bold="true" box="[809,1106,1426,1452]" pageId="9" pageNumber="22">Ecological relationships</emphasis>
</heading>
</paragraph>
<paragraph id="27386E717700FFEEFC8AAC9093604C5F" blockId="9.[809,1456,1475,1947]" pageId="9" pageNumber="22">
With some exceptions, the oxycline and hypolimnion layers, where 
<taxonomicName id="E08715F27700FFEEFBC9ACB593724EB0" box="[1036,1123,1510,1536]" class="Heterotrichea" family="Spirostomidae" genus="Spirostomum" kingdom="Chromista" order="Heterotrichida" pageId="9" pageNumber="22" phylum="Ciliophora" rank="species" species="teres">
<emphasis id="15F3B2637700FFEEFBC9ACB593724EB0" box="[1036,1123,1510,1536]" italics="true" pageId="9" pageNumber="22">S. teres</emphasis>
</taxonomicName>
was found, showed oxygen concentration below 
<quantity id="E07FC3947700FFEEFBD9AF5B93444D6D" box="[1052,1109,1544,1570]" metricMagnitude="-6" metricUnit="kg" metricValue="1.0" pageId="9" pageNumber="22" unit="mg" value="1.0">1 mg</quantity>
<collectionCode id="4196F6B47700FFEEFB9EAF5B937C4D6D" box="[1115,1133,1544,1570]" country="Netherlands" lsid="urn:lsid:biocol.org:col:15678" name="Nationaal Herbarium Nederland, Leiden University branch" pageId="9" pageNumber="22" type="Herbarium">L</collectionCode>
<superScript id="D0F2C3397700FFEEFBA8AF54936F4D5A" attach="left" box="[1133,1150,1543,1557]" fontSize="6" pageId="9" pageNumber="22">–1</superScript>
with maximum over 2000 cells 
<collectionCode id="4196F6B47700FFEEFCADAF79946B4D0B" box="[872,890,1578,1604]" country="Netherlands" lsid="urn:lsid:biocol.org:col:15678" name="Nationaal Herbarium Nederland, Leiden University branch" pageId="9" pageNumber="22" type="Herbarium">L</collectionCode>
<superScript id="D0F2C3397700FFEEFCBFAF7A949A4D78" attach="left" box="[890,907,1577,1591]" fontSize="6" pageId="9" pageNumber="22">–1</superScript>
at 
<collectionCode id="4196F6B47700FFEEFC76AF7A94CC4D0C" box="[947,989,1577,1603]" country="France" lsid="urn:lsid:biocol.org:col:14073" name="Société d'Agriculture Sciences et Arts" pageId="9" pageNumber="22" type="Herbarium">DO</collectionCode>
of 0.2 mgL 
<superScript id="D0F2C3397700FFEEFBABAF7A936E4D78" attach="left" box="[1134,1151,1577,1591]" fontSize="6" pageId="9" pageNumber="22">–1</superScript>
and lower (
<figureCitation id="BFBC72F47700FFEEFAD7AF7A927B4D0C" box="[1298,1386,1577,1603]" captionStart="Fig" captionStartId="15.[125,160,892,914]" captionTargetBox="[133,756,232,852]" captionTargetId="figure-8@15.[125,771,223,859]" captionTargetPageId="15" captionText="Fig. 11. Spirostomum teres occurrence (%) in habitats defined by DO concentration (mgL–1)." figureDoi="http://doi.org/10.5281/zenodo.10994497" httpUri="https://zenodo.org/record/10994497/files/figure.png" pageId="9" pageNumber="22">Fig. 11</figureCitation>
). Notoriously, 
<taxonomicName id="E08715F27700FFEEFC5BAF1F94E54D2A" box="[926,1012,1612,1638]" class="Heterotrichea" family="Spirostomidae" genus="Spirostomum" kingdom="Chromista" order="Heterotrichida" pageId="9" pageNumber="22" phylum="Ciliophora" rank="species" species="teres">
<emphasis id="15F3B2637700FFEEFC5BAF1F94E54D2A" box="[926,1012,1612,1638]" italics="true" pageId="9" pageNumber="22">S. teres</emphasis>
</taxonomicName>
was found at 
<collectionCode id="4196F6B47700FFEEFB5FAF1893D54D2A" box="[1178,1220,1611,1637]" country="France" lsid="urn:lsid:biocol.org:col:14073" name="Société d'Agriculture Sciences et Arts" pageId="9" pageNumber="22" type="Herbarium">DO</collectionCode>
below the accuracy limit within the completely anoxic hypolimnion. The ciliates were observed regularly at the very bottom including in anaerobic conditions until 2011, when large number of 1900 cells 
<collectionCode id="4196F6B47700FFEEFBEAAF8793504DA1" box="[1071,1089,1748,1774]" country="Netherlands" lsid="urn:lsid:biocol.org:col:15678" name="Nationaal Herbarium Nederland, Leiden University branch" pageId="9" pageNumber="22" type="Herbarium">L</collectionCode>
<superScript id="D0F2C3397700FFEEFB84AF8193434DAF" attach="left" box="[1089,1106,1746,1760]" fontSize="6" pageId="9" pageNumber="22">–1</superScript>
was found. 
<taxonomicName id="E08715F27700FFEEFB23AF80922C4DA2" box="[1254,1341,1747,1773]" class="Heterotrichea" family="Spirostomidae" genus="Spirostomum" kingdom="Chromista" order="Heterotrichida" pageId="9" pageNumber="22" phylum="Ciliophora" rank="species" species="teres">
<emphasis id="15F3B2637700FFEEFB23AF80922C4DA2" box="[1254,1341,1747,1773]" italics="true" pageId="9" pageNumber="22">S. teres</emphasis>
</taxonomicName>
appeared there again in 2016 (
<figureCitation id="BFBC72F47700FFEEFBDEAFA593714C5F" box="[1051,1120,1782,1808]" captionStart="Fig" captionStartId="6.[132,167,1102,1124]" captionTargetBox="[150,1460,234,1043]" captionTargetId="figure-6@6.[136,1466,219,1060]" captionTargetPageId="6" captionText="Fig. 3. a) Dissolved Oxygen isopleths, sampled data/depths (Analysed), and of Spirostomum teres numbers in Lake Alchichica; b) average S. teres numbers throughout an hypoxic/anoxic layer (based on Peštová et al. 2008, Bautista-Reyes and Macek 2012, Sánchez-Medina et al. 2016, and this study)." figureDoi="http://doi.org/10.5281/zenodo.10994481" httpUri="https://zenodo.org/record/10994481/files/figure.png" pageId="9" pageNumber="22">Fig. 3</figureCitation>
).
</paragraph>
<paragraph id="27386E717700FFEBFC8AAE4A96C34CDB" blockId="9.[809,1456,1475,1947]" lastBlockId="12.[132,779,1706,1941]" lastPageId="12" lastPageNumber="25" pageId="9" pageNumber="22">
To see a possible anaerobic respiration within the sampled layers, 
<taxonomicName id="E08715F27700FFEEFC2DAE6F932F4C1A" box="[1000,1086,1852,1878]" class="Heterotrichea" family="Spirostomidae" genus="Spirostomum" kingdom="Chromista" order="Heterotrichida" pageId="9" pageNumber="22" phylum="Ciliophora" rank="species" species="teres">
<emphasis id="15F3B2637700FFEEFC2DAE6F932F4C1A" box="[1000,1086,1852,1878]" italics="true" pageId="9" pageNumber="22">S. teres</emphasis>
</taxonomicName>
numbers were plot against 
<collectionCode id="4196F6B47700FFEEFA40AE6892BE4C1A" box="[1413,1455,1851,1877]" country="France" lsid="urn:lsid:biocol.org:col:14073" name="Société d'Agriculture Sciences et Arts" pageId="9" pageNumber="22" type="Herbarium">DO</collectionCode>
and a nitrate concentration (
<figureCitation id="BFBC72F47700FFEEFB4CAE0D93F64C37" box="[1161,1255,1886,1912]" captionStart="Fig" captionStartId="16.[132,167,1423,1445]" captionTargetBox="[173,1411,268,1368]" captionTargetId="figure-233@16.[132,1462,223,1394]" captionTargetPageId="16" captionText="Fig. 12. a) Spirostomum teres numbers plot [cells mL–1] against dissolved oxygen, DO [mg L–1] vs. nitrate nitrogen NO – [µmolL–1], and 3 all analysed DO/nitrate data, b) Species occurrence in habitats, in which S. teres was found (in the logarithmic scale of nitrate nitrogen concentration)." figureDoi="http://doi.org/10.5281/zenodo.10994499" httpUri="https://zenodo.org/record/10994499/files/figure.png" pageId="9" pageNumber="22">Fig. 12</figureCitation>
). 
<taxonomicName id="E08715F27700FFEEFAC7AE0D924C4C37" box="[1282,1373,1886,1912]" class="Heterotrichea" family="Spirostomidae" genus="Spirostomum" kingdom="Chromista" order="Heterotrichida" pageId="9" pageNumber="22" phylum="Ciliophora" rank="species" species="teres">
<emphasis id="15F3B2637700FFEEFAC7AE0D924C4C37" box="[1282,1373,1886,1912]" italics="true" pageId="9" pageNumber="22">S. teres</emphasis>
</taxonomicName>
maximums were found upon concentrations over 1 µmol 
<collectionCode id="4196F6B47700FFEEFA49AED2928F4CD4" box="[1420,1438,1921,1947]" country="Netherlands" lsid="urn:lsid:biocol.org:col:15678" name="Nationaal Herbarium Nederland, Leiden University branch" pageId="9" pageNumber="22" type="Herbarium">L</collectionCode>
<superScript id="D0F2C3397700FFEEFA5BAED392BE4CC1" attach="left" box="[1438,1455,1920,1934]" fontSize="6" pageId="9" pageNumber="22">–1</superScript>
both with traces of 
<collectionCode id="4196F6B47705FFEBFEBCAFF896B24D8A" box="[377,419,1707,1733]" country="France" lsid="urn:lsid:biocol.org:col:14073" name="Société d'Agriculture Sciences et Arts" pageId="12" pageNumber="25" type="Herbarium">DO</collectionCode>
(0.1 through 0.2 mgL 
<superScript id="D0F2C3397705FFEBFD7CAFF995DB4DF7" attach="left" box="[697,714,1706,1720]" fontSize="6" pageId="12" pageNumber="25">–1</superScript>
) and at an anaerobic bottom. Also according to the occurrence of 
<taxonomicName id="E08715F27705FFEBFF2FAFA396514C45" box="[234,320,1776,1802]" class="Heterotrichea" family="Spirostomidae" genus="Spirostomum" kingdom="Chromista" order="Heterotrichida" pageId="12" pageNumber="25" phylum="Ciliophora" rank="species" species="teres">
<emphasis id="15F3B2637705FFEBFF2FAFA396514C45" box="[234,320,1776,1802]" italics="true" pageId="12" pageNumber="25">S. teres</emphasis>
</taxonomicName>
(percentage of positive samples within a logarithmic scale of concentration intervals), the ciliate preferred nitrate concentrations between 0.6 and 2.5 µmolL 
<superScript id="D0F2C3397705FFEBFF10AE0497F74C2A" attach="left" box="[213,230,1879,1893]" fontSize="6" pageId="12" pageNumber="25">–1</superScript>
while upon concentrations over 6.3 µmolL 
<superScript id="D0F2C3397705FFEBFD3CAE04941B4C2A" attach="left" box="[761,778,1879,1893]" fontSize="6" pageId="12" pageNumber="25">–1</superScript>
the ciliate was not observed.
</paragraph>
<caption id="73F83EF97703FFEDFF4DAE0B95364CC5" ID-DOI="http://doi.org/10.5281/zenodo.10994487" ID-Zenodo-Dep="10994487" httpUri="https://zenodo.org/record/10994487/files/figure.png" pageId="10" pageNumber="23" startId="10.[136,171,1880,1902]" targetBox="[253,1330,223,1861]" targetPageId="10" targetType="figure">
<paragraph id="27386E717703FFEDFF4DAE0B95364CC5" blockId="10.[136,1467,1880,1931]" pageId="10" pageNumber="23">
<emphasis id="15F3B2637703FFEDFF4DAE0B97D64C22" bold="true" box="[136,199,1880,1902]" pageId="10" pageNumber="23">Fig. 6.</emphasis>
<taxonomicName id="E08715F27703FFEDFF09AE0A966F4C21" box="[204,382,1881,1902]" class="Heterotrichea" family="Spirostomidae" genus="Spirostomum" kingdom="Chromista" order="Heterotrichida" pageId="10" pageNumber="23" phylum="Ciliophora" rank="species" species="teres">
<emphasis id="15F3B2637703FFEDFF09AE0A966F4C21" box="[204,382,1881,1902]" italics="true" pageId="10" pageNumber="23">Spirostomum teres</emphasis>
</taxonomicName>
: 
<emphasis id="15F3B2637703FFEDFE4FAE0B968F4C21" bold="true" box="[394,414,1880,1902]" pageId="10" pageNumber="23">a)</emphasis>
Lake Alchichica (8/8/2011, 30 m), 
<emphasis id="15F3B2637703FFEDFD34AE0B94174C21" bold="true" box="[753,774,1880,1902]" pageId="10" pageNumber="23">b)</emphasis>
Lake La Preciosa (2/9/2011, 20.5 m and 
<emphasis id="15F3B2637703FFEDFB4BAE0B93B04C21" bold="true" box="[1166,1185,1880,1902]" pageId="10" pageNumber="23">c)</emphasis>
Lake de La Cruz (25/6/2010, 11.25 m. Protargol stain (QPS). 1 ≡ 10 µm.
</paragraph>
</caption>
<caption id="73F83EF97702FFECFFB8AE37955B4CD9" ID-DOI="http://doi.org/10.5281/zenodo.10994489" ID-Zenodo-Dep="10994489" httpUri="https://zenodo.org/record/10994489/files/figure.png" pageId="11" pageNumber="24" startId="11.[125,160,1892,1914]" targetBox="[225,1346,223,1859]" targetPageId="11" targetType="figure">
<paragraph id="27386E717702FFECFFB8AE37955B4CD9" blockId="11.[125,1455,1892,1942]" pageId="11" pageNumber="24">
<emphasis id="15F3B2637702FFECFFB8AE3797AD4C36" bold="true" box="[125,188,1892,1914]" pageId="11" pageNumber="24">Fig. 7.</emphasis>
<taxonomicName id="E08715F27702FFECFF04AE3796634C35" box="[193,370,1892,1914]" class="Heterotrichea" family="Spirostomidae" genus="Spirostomum" kingdom="Chromista" order="Heterotrichida" pageId="11" pageNumber="24" phylum="Ciliophora" rank="species" species="teres">
<emphasis id="15F3B2637702FFECFF04AE3796634C35" box="[193,370,1892,1914]" italics="true" pageId="11" pageNumber="24">Spirostomum teres</emphasis>
</taxonomicName>
in Alchichica on July 2016 (epifluorescence microscope, Leica DMLB). 
<emphasis id="15F3B2637702FFECFBEEAE37932E4C35" bold="true" box="[1067,1087,1892,1914]" pageId="11" pageNumber="24">a)</emphasis>
DAPI staining; 
<emphasis id="15F3B2637702FFECFB1FAE3793FE4C35" bold="true" box="[1242,1263,1892,1914]" pageId="11" pageNumber="24">b)</emphasis>
Phycobilin filter set (Y3); 
<emphasis id="15F3B2637702FFECFF72AED397D84CD9" bold="true" box="[183,201,1920,1942]" pageId="11" pageNumber="24">c)</emphasis>
Chlorophyll 
<emphasis id="15F3B2637702FFECFE8CAED296444CD9" box="[329,341,1921,1942]" italics="true" pageId="11" pageNumber="24">a</emphasis>
filter set (I3). 1 ≡ 10 µm.
</paragraph>
</caption>
<caption id="73F83EF97705FFEBFF41AA1895634832" ID-DOI="http://doi.org/10.5281/zenodo.10994491" ID-Zenodo-Dep="10994491" httpUri="https://zenodo.org/record/10994491/files/figure.png" pageId="12" pageNumber="25" startId="12.[132,167,843,865]" targetBox="[132,1462,223,815]" targetPageId="12" targetType="figure">
<paragraph id="27386E717705FFEBFF41AA1895634832" blockId="12.[132,1462,843,893]" pageId="12" pageNumber="25">
<emphasis id="15F3B2637705FFEBFF41AA1897D2482F" bold="true" box="[132,195,843,865]" pageId="12" pageNumber="25">Fig. 8.</emphasis>
<taxonomicName id="E08715F27705FFEBFF0DAA189669482E" box="[200,376,843,865]" class="Heterotrichea" family="Spirostomidae" genus="Spirostomum" kingdom="Chromista" order="Heterotrichida" pageId="12" pageNumber="25" phylum="Ciliophora" rank="species" species="teres">
<emphasis id="15F3B2637705FFEBFF0DAA189669482E" box="[200,376,843,865]" italics="true" pageId="12" pageNumber="25">Spirostomum teres</emphasis>
</taxonomicName>
in Alchichica on July 2013 (epifluorescence microscope, Leica DMLB). 
<emphasis id="15F3B2637705FFEBFBE8AA189350482E" bold="true" box="[1069,1089,843,865]" pageId="12" pageNumber="25">a)</emphasis>
DAPI filter set (A); 
<emphasis id="15F3B2637705FFEBFAC7AA189206482E" bold="true" box="[1282,1303,843,865]" pageId="12" pageNumber="25">b)</emphasis>
Phycobilin filter set (Y3); 
<emphasis id="15F3B2637705FFEBFF1AAA3497E04832" bold="true" box="[223,241,871,893]" pageId="12" pageNumber="25">c)</emphasis>
Chlorophyll 
<emphasis id="15F3B2637705FFEBFEB7AA3B966F4832" box="[370,382,872,893]" italics="true" pageId="12" pageNumber="25">a</emphasis>
filter set (I3). 1 ≡ 10 µm.
</paragraph>
</caption>
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<paragraph id="27386E717705FFEBFF41AF7995F34D13" blockId="12.[132,1463,1578,1628]" pageId="12" pageNumber="25">
<emphasis id="15F3B2637705FFEBFF41AF7997D24D70" bold="true" box="[132,195,1578,1600]" pageId="12" pageNumber="25">Fig. 9.</emphasis>
Lake Alchichica 
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<emphasis id="15F3B2637705FFEBFEAFAF78950A4D0F" box="[362,539,1579,1600]" italics="true" pageId="12" pageNumber="25">Spirostomum teres</emphasis>
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with apparent purple sulphur bacteria in Protargol stain 
<emphasis id="15F3B2637705FFEBFBF7AF7993574D0F" bold="true" box="[1074,1094,1578,1600]" pageId="12" pageNumber="25">a)</emphasis>
4/12/2012, 36.5 m; 
<emphasis id="15F3B2637705FFEBFAC3AF79920A4D0F" bold="true" box="[1286,1307,1578,1600]" pageId="12" pageNumber="25">b)</emphasis>
19/11/14, 38 m, and in infrared autofluorescence 
<emphasis id="15F3B2637705FFEBFE7AAF1596C54D13" bold="true" box="[447,468,1606,1628]" pageId="12" pageNumber="25">b)</emphasis>
19/11/14, 38 m. 1 ≡ 10 µm.
</paragraph>
</caption>
<paragraph id="27386E717705FFE9FC93AFF895AF4C01" blockId="12.[816,1463,1707,1941]" lastBlockId="14.[132,779,1740,1940]" lastPageId="14" lastPageNumber="27" pageId="12" pageNumber="25">
The same plot but applying the concentration of nitrite (
<figureCitation id="BFBC72F47705FFEBFCB1AF9E94DC4DA8" box="[884,973,1741,1767]" captionStart="Fig" captionStartId="17.[125,160,1434,1456]" captionTargetBox="[140,1441,245,1385]" captionTargetId="figure-8@17.[125,1456,223,1411]" captionTargetPageId="17" captionText="Fig. 13. a) Spirostomum teres numbers plot [cells mL–1] against dissolved oxygen, DO [mg L–1] vs. nitrite nitrogen NO – [µmolL–1], and all 2 analysed DO/nutrients data plot, b) Species occurrence in habitats, in which S. teres was found (in the logarithmic scale of nitrite nitrogen concentration)." figureDoi="http://doi.org/10.5281/zenodo.10994501" httpUri="https://zenodo.org/record/10994501/files/figure.png" pageId="12" pageNumber="25">Fig. 13</figureCitation>
), a possible respiration product, did not give clear results; nitrites&gt; 1 µmol 
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coexisted with 
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&gt; 
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/l but the ciliate was extremely scarce in such conditions. According to the occurrence of 
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<emphasis id="15F3B2637705FFEBFAEBAE6692954C00" box="[1326,1412,1845,1871]" italics="true" pageId="12" pageNumber="25">S. teres</emphasis>
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, the ciliate preferred nitrite concentrations between 0.1 and 1 µmol 
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while hypolimnetic 
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<emphasis id="15F3B2637705FFEBFB5EAE2993FE4CDB" box="[1179,1263,1914,1940]" italics="true" pageId="12" pageNumber="25">S. teres</emphasis>
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maximums were observed between 1 and 2 µmolL 
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. The ciliate occurrence was higher again over 2 µmol 
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– 
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the completely anaerobic conditions but the ciliate numbers were not considerably higher in such conditions.
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</th>
<th id="DA66054F77040018FC93A855948A4A6D" box="[854,923,262,290]" gridcol="4" gridrow="0" pageId="13" pageNumber="26">N</th>
<th id="DA66054F77040018FC68A85593234A6D" box="[941,1074,262,290]" gridcol="5" gridrow="0" pageId="13" pageNumber="26">F</th>
<th id="DA66054F77040018FB83A85593804A6D" box="[1094,1169,262,290]" gridcol="6" gridrow="0" pageId="13" pageNumber="26">SD</th>
<th id="DA66054F77040018FB60A85592184A6D" box="[1189,1289,262,290]" gridcol="7" gridrow="0" pageId="13" pageNumber="26">G</th>
<th id="DA66054F77040018FAEAA85592654A6D" box="[1327,1396,262,290]" gridcol="8" gridrow="0" pageId="13" pageNumber="26">
<emphasis id="15F3B2637704FFEAFAF6A855924D4A6D" bold="true" box="[1331,1372,262,290]" pageId="13" pageNumber="26">SD</emphasis>
</th>
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<th id="DA66054F77040018FF7CA87B96454A05" box="[185,340,296,330]" gridcol="0" gridrow="1" pageId="13" pageNumber="26">lake</th>
<td id="DA66054F77040018FEAFA87B96EF4A05" box="[362,510,296,330]" gridcol="1" gridrow="1" pageId="13" pageNumber="26">date</td>
<td id="DA66054F77040018FDD7A87B95B24A05" box="[530,675,296,330]" gridcol="2" gridrow="1" pageId="13" pageNumber="26">depth</td>
<td id="DA66054F77040018FD7FA87B94514A05" box="[698,832,296,330]" gridcol="3" gridrow="1" pageId="13" pageNumber="26">
<emphasis id="15F3B2637704FFEAFD7FA87D94274A75" bold="true" box="[698,822,296,330]" pageId="13" pageNumber="26">
cells 
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</emphasis>
1
</td>
<td id="DA66054F77040018FC93A87B948A4A05" box="[854,923,296,330]" gridcol="4" gridrow="1" pageId="13" pageNumber="26">cells</td>
<td id="DA66054F77040018FC68A87B93804A05" box="[941,1169,296,330]" colspan="2" colspanRight="1" gridcol="5" gridrow="1" pageId="13" pageNumber="26">
10 
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<emphasis id="15F3B2637704FFEAFC18A87D93724A75" bold="true" box="[989,1123,296,330]" pageId="13" pageNumber="26">
nL 
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</emphasis>
1 
<superScript id="D0F2C3397704FFEAFBA8A87D939E4A75" attach="left" box="[1133,1167,296,330]" fontSize="7" pageId="13" pageNumber="26">h-1</superScript>
</td>
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<emphasis id="15F3B2637704FFEAFB60A87D92524A75" bold="true" box="[1189,1347,296,330]" pageId="13" pageNumber="26">
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<superScript id="D0F2C3397704FFEAFB2FA87D92524A75" attach="both" box="[1258,1347,296,330]" fontSize="7" pageId="13" pageNumber="26">ciliate-</superScript>
</emphasis>
1 
<emphasis id="15F3B2637704FFEAFA8BA87D92614A75" bold="true" box="[1358,1392,296,330]" pageId="13" pageNumber="26">
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<emphasis id="15F3B2637704FFEAFA8BA87D92774A75" bold="true" box="[1358,1382,296,330]" pageId="13" pageNumber="26">h-</emphasis>
1
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</emphasis>
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</tr>
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<th id="DA66054F77040018FF7CA80396454A3E" box="[185,340,336,369]" gridcol="0" gridrow="2" pageId="13" pageNumber="26">Alchichica</th>
<td id="DA66054F77040018FEAFA80396EF4A3E" box="[362,510,336,369]" gridcol="1" gridrow="2" pageId="13" pageNumber="26">
<date id="533948B17704FFEAFEAFA80596EC4A3E" box="[362,509,342,369]" pageId="13" pageNumber="26" value="2011-10-25">25/10/2011</date>
</td>
<td id="DA66054F77040018FDD7A80395B24A3E" box="[530,675,336,369]" gridcol="2" gridrow="2" pageId="13" pageNumber="26">29</td>
<td id="DA66054F77040018FD7FA80394514A3E" box="[698,832,336,369]" gridcol="3" gridrow="2" pageId="13" pageNumber="26">
1.96×10 
<superScript id="D0F2C3397704FFEAFCEBA80394294A2D" attach="left" box="[814,824,336,354]" fontSize="7" pageId="13" pageNumber="26">5</superScript>
</td>
<td id="DA66054F77040018FC93A803948A4A3E" box="[854,923,336,369]" gridcol="4" gridrow="2" pageId="13" pageNumber="26">7</td>
<td id="DA66054F77040018FC68A80393234A3E" box="[941,1074,336,369]" gridcol="5" gridrow="2" pageId="13" pageNumber="26">0.80</td>
<td id="DA66054F77040018FB83A80393804A3E" box="[1094,1169,336,369]" gridcol="6" gridrow="2" pageId="13" pageNumber="26">0.71</td>
<td id="DA66054F77040018FB60A80392184A3E" box="[1189,1289,336,369]" gridcol="7" gridrow="2" pageId="13" pageNumber="26">217</td>
<td id="DA66054F77040018FAEAA80392654A3E" box="[1327,1396,336,369]" gridcol="8" gridrow="2" pageId="13" pageNumber="26">195</td>
</tr>
<tr id="99B76C3377040018FF7CA8F392654A8E" box="[185,1396,416,449]" gridrow="3" pageId="13" pageNumber="26">
<th id="DA66054F77040018FF7CA8F396454A8E" box="[185,340,416,449]" gridcol="0" gridrow="3" pageId="13" pageNumber="26">Alchichica</th>
<td id="DA66054F77040018FEAFA8F396EF4A8E" box="[362,510,416,449]" gridcol="1" gridrow="3" pageId="13" pageNumber="26">
<date id="533948B17704FFEAFEAFA8F596EC4A8E" box="[362,509,422,449]" pageId="13" pageNumber="26" value="2011-11-15">15/11/2011</date>
</td>
<td id="DA66054F77040018FDD7A8F395B24A8E" box="[530,675,416,449]" gridcol="2" gridrow="3" pageId="13" pageNumber="26">30</td>
<td id="DA66054F77040018FD7FA8F394514A8E" box="[698,832,416,449]" gridcol="3" gridrow="3" pageId="13" pageNumber="26">
2.20×10 
<superScript id="D0F2C3397704FFEAFCEBA8F394294AFD" attach="none" box="[814,824,416,434]" fontSize="7" pageId="13" pageNumber="26">5</superScript>
</td>
<td id="DA66054F77040018FC93A8F3948A4A8E" box="[854,923,416,449]" gridcol="4" gridrow="3" pageId="13" pageNumber="26">7</td>
<td id="DA66054F77040018FC68A8F393234A8E" box="[941,1074,416,449]" gridcol="5" gridrow="3" pageId="13" pageNumber="26">0.41</td>
<td id="DA66054F77040018FB83A8F393804A8E" box="[1094,1169,416,449]" gridcol="6" gridrow="3" pageId="13" pageNumber="26">0.18</td>
<td id="DA66054F77040018FB60A8F392184A8E" box="[1189,1289,416,449]" gridcol="7" gridrow="3" pageId="13" pageNumber="26">135</td>
<td id="DA66054F77040018FAEAA8F392654A8E" box="[1327,1396,416,449]" gridcol="8" gridrow="3" pageId="13" pageNumber="26">60</td>
</tr>
<tr id="99B76C3377040018FF7CA8A39265495E" box="[185,1396,496,529]" gridrow="4" pageId="13" pageNumber="26">
<th id="DA66054F77040018FF7CA8A39645495E" box="[185,340,496,529]" gridcol="0" gridrow="4" pageId="13" pageNumber="26">Alchichica</th>
<td id="DA66054F77040018FEAFA8A396EF495E" box="[362,510,496,529]" gridcol="1" gridrow="4" pageId="13" pageNumber="26">
<date id="533948B17704FFEAFEAFA8A596EC495E" box="[362,509,502,529]" pageId="13" pageNumber="26" value="2012-09-11">11/09/2012</date>
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<td id="DA66054F77040018FDD7A8A395B2495E" box="[530,675,496,529]" gridcol="2" gridrow="4" pageId="13" pageNumber="26">25</td>
<td id="DA66054F77040018FD7FA8A39451495E" box="[698,832,496,529]" gridcol="3" gridrow="4" pageId="13" pageNumber="26">
5.51×10 
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</td>
<td id="DA66054F77040018FC93A8A3948A495E" box="[854,923,496,529]" gridcol="4" gridrow="4" pageId="13" pageNumber="26">37</td>
<td id="DA66054F77040018FC68A8A39323495E" box="[941,1074,496,529]" gridcol="5" gridrow="4" pageId="13" pageNumber="26">1.70</td>
<td id="DA66054F77040018FB83A8A39380495E" box="[1094,1169,496,529]" gridcol="6" gridrow="4" pageId="13" pageNumber="26">0.56</td>
<td id="DA66054F77040018FB60A8A39218495E" box="[1189,1289,496,529]" gridcol="7" gridrow="4" pageId="13" pageNumber="26">136</td>
<td id="DA66054F77040018FAEAA8A39265495E" box="[1327,1396,496,529]" gridcol="8" gridrow="4" pageId="13" pageNumber="26">45</td>
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<tr id="99B76C3377040018FF7CAB4B92654976" box="[185,1396,536,569]" gridrow="5" pageId="13" pageNumber="26" rowspan-0="1" rowspan-1="1">
<td id="DA66054F77040018FDD7AB4B95B24976" box="[530,675,536,569]" gridcol="2" gridrow="5" pageId="13" pageNumber="26">26</td>
<td id="DA66054F77040018FD7FAB4B94514976" box="[698,832,536,569]" gridcol="3" gridrow="5" pageId="13" pageNumber="26">
5.84×10 
<superScript id="D0F2C3397704FFEAFCEBAB4B94294965" attach="right" box="[814,824,536,554]" fontSize="7" pageId="13" pageNumber="26">4</superScript>
</td>
<td id="DA66054F77040018FC93AB4B948A4976" box="[854,923,536,569]" gridcol="4" gridrow="5" pageId="13" pageNumber="26">19</td>
<td id="DA66054F77040018FC68AB4B93234976" box="[941,1074,536,569]" gridcol="5" gridrow="5" pageId="13" pageNumber="26">2.78</td>
<td id="DA66054F77040018FB83AB4B93804976" box="[1094,1169,536,569]" gridcol="6" gridrow="5" pageId="13" pageNumber="26">0.92</td>
<td id="DA66054F77040018FB60AB4B92184976" box="[1189,1289,536,569]" gridcol="7" gridrow="5" pageId="13" pageNumber="26">227</td>
<td id="DA66054F77040018FAEAAB4B92654976" box="[1327,1396,536,569]" gridcol="8" gridrow="5" pageId="13" pageNumber="26">75</td>
</tr>
<tr id="99B76C3377040018FF7CAB159265492E" box="[185,1396,582,609]" gridrow="6" pageId="13" pageNumber="26" rowspan-0="1" rowspan-1="1">
<td id="DA66054F77040018FDD7AB1395B2492E" box="[530,675,576,609]" gridcol="2" gridrow="6" pageId="13" pageNumber="26">27</td>
<td id="DA66054F77040018FD7FAB139451492E" box="[698,832,576,609]" gridcol="3" gridrow="6" pageId="13" pageNumber="26">
3.21×10 
<superScript id="D0F2C3397704FFEAFCEBAB139429491D" attach="right" box="[814,824,576,594]" fontSize="7" pageId="13" pageNumber="26">4</superScript>
</td>
<td id="DA66054F77040018FC93AB13948A492E" box="[854,923,576,609]" gridcol="4" gridrow="6" pageId="13" pageNumber="26">34</td>
<td id="DA66054F77040018FC68AB139323492E" box="[941,1074,576,609]" gridcol="5" gridrow="6" pageId="13" pageNumber="26">2.28</td>
<td id="DA66054F77040018FB83AB139380492E" box="[1094,1169,576,609]" gridcol="6" gridrow="6" pageId="13" pageNumber="26">0.68</td>
<td id="DA66054F77040018FB60AB139218492E" box="[1189,1289,576,609]" gridcol="7" gridrow="6" pageId="13" pageNumber="26">122</td>
<td id="DA66054F77040018FAEAAB139265492E" box="[1327,1396,576,609]" gridcol="8" gridrow="6" pageId="13" pageNumber="26">36</td>
</tr>
<tr id="99B76C3377040018FF7CAB3D926549C6" box="[185,1396,622,649]" gridrow="7" pageId="13" pageNumber="26" rowspan-0="1" rowspan-1="1">
<td id="DA66054F77040018FDD7AB3D95B249C6" box="[530,675,622,649]" gridcol="2" gridrow="7" pageId="13" pageNumber="26">28</td>
<td id="DA66054F77040018FD7FAB3D945149C6" box="[698,832,622,649]" gridcol="3" gridrow="7" pageId="13" pageNumber="26">
3.96×10 
<superScript id="D0F2C3397704FFEAFCEBAB3B94294935" attach="right" box="[814,824,616,634]" fontSize="7" pageId="13" pageNumber="26">4</superScript>
</td>
<td id="DA66054F77040018FC93AB3D948A49C6" box="[854,923,622,649]" gridcol="4" gridrow="7" pageId="13" pageNumber="26">19</td>
<td id="DA66054F77040018FC68AB3D932349C6" box="[941,1074,622,649]" gridcol="5" gridrow="7" pageId="13" pageNumber="26">2.24</td>
<td id="DA66054F77040018FB83AB3D938049C6" box="[1094,1169,622,649]" gridcol="6" gridrow="7" pageId="13" pageNumber="26">1.12</td>
<td id="DA66054F77040018FB60AB3D921849C6" box="[1189,1289,622,649]" gridcol="7" gridrow="7" pageId="13" pageNumber="26">152</td>
<td id="DA66054F77040018FAEAAB3D926549C6" box="[1327,1396,622,649]" gridcol="8" gridrow="7" pageId="13" pageNumber="26">76</td>
</tr>
<tr id="99B76C3377040018FF7CABC0926549E0" box="[185,1396,659,687]" gridrow="8" pageId="13" pageNumber="26" rowspan-0="1" rowspan-1="1" rowspan-3="1">
<td id="DA66054F77040018FDD7ABC095B249E0" box="[530,675,659,687]" gridcol="2" gridrow="8" pageId="13" pageNumber="26">average</td>
<td id="DA66054F77040018FC93ABC0948A49E0" box="[854,923,659,687]" gridcol="4" gridrow="8" pageId="13" pageNumber="26">109</td>
<td id="DA66054F77040018FC68ABC0932349E0" box="[941,1074,659,687]" gridcol="5" gridrow="8" pageId="13" pageNumber="26">2.16</td>
<td id="DA66054F77040018FB83ABC0938049E0" box="[1094,1169,659,687]" gridcol="6" gridrow="8" pageId="13" pageNumber="26">0.76</td>
<td id="DA66054F77040018FB60ABC0921849E0" box="[1189,1289,659,687]" gridcol="7" gridrow="8" pageId="13" pageNumber="26">150</td>
<td id="DA66054F77040018FAEAABC0926549E0" box="[1327,1396,659,687]" gridcol="8" gridrow="8" pageId="13" pageNumber="26">
<emphasis id="15F3B2637704FFEAFAF2ABC0924949E0" box="[1335,1368,659,687]" italics="true" pageId="13" pageNumber="26">53</emphasis>
</td>
</tr>
<tr id="99B76C3377040018FF7CABB0926549B0" box="[185,1396,739,767]" gridrow="9" pageId="13" pageNumber="26">
<th id="DA66054F77040018FF7CAB8E964549B0" box="[185,340,733,767]" gridcol="0" gridrow="9" pageId="13" pageNumber="26">Alchichica</th>
<td id="DA66054F77040018FEAFAB8E96EF49B0" box="[362,510,733,767]" gridcol="1" gridrow="9" pageId="13" pageNumber="26">
<date id="533948B17704FFEAFEAFABB096EF49B0" box="[362,510,739,767]" pageId="13" pageNumber="26" value="2012-10-24">24/10/2012</date>
</td>
<td id="DA66054F77040018FDD7AB8E95B249B0" box="[530,675,733,767]" gridcol="2" gridrow="9" pageId="13" pageNumber="26">27</td>
<td id="DA66054F77040018FD7FAB8E945149B0" box="[698,832,733,767]" gridcol="3" gridrow="9" pageId="13" pageNumber="26">
1.45×10 
<superScript id="D0F2C3397704FFEAFCEBAB8E942949A0" attach="right" box="[814,824,733,751]" fontSize="7" pageId="13" pageNumber="26">5</superScript>
</td>
<td id="DA66054F77040018FC93AB8E948A49B0" box="[854,923,733,767]" gridcol="4" gridrow="9" pageId="13" pageNumber="26">4</td>
<td id="DA66054F77040018FC68AB8E932349B0" box="[941,1074,733,767]" gridcol="5" gridrow="9" pageId="13" pageNumber="26">1.22</td>
<td id="DA66054F77040018FB83AB8E938049B0" box="[1094,1169,733,767]" gridcol="6" gridrow="9" pageId="13" pageNumber="26">0.33</td>
<td id="DA66054F77040018FB60AB8E921849B0" box="[1189,1289,733,767]" gridcol="7" gridrow="9" pageId="13" pageNumber="26">196</td>
<td id="DA66054F77040018FAEAAB8E926549B0" box="[1327,1396,733,767]" gridcol="8" gridrow="9" pageId="13" pageNumber="26">54</td>
</tr>
<tr id="99B76C3377040018FF7CAA5892654868" box="[185,1396,779,807]" gridrow="10" pageId="13" pageNumber="26" rowspan-0="1" rowspan-1="1">
<td id="DA66054F77040018FDD7AA5895B24868" box="[530,675,779,807]" gridcol="2" gridrow="10" pageId="13" pageNumber="26">28</td>
<td id="DA66054F77040018FD7FAA5894514868" box="[698,832,779,807]" gridcol="3" gridrow="10" pageId="13" pageNumber="26">
1.01×10 
<superScript id="D0F2C3397704FFEAFCEBAA5694294858" attach="right" box="[814,824,773,791]" fontSize="7" pageId="13" pageNumber="26">5</superScript>
</td>
<td id="DA66054F77040018FC93AA58948A4868" box="[854,923,779,807]" gridcol="4" gridrow="10" pageId="13" pageNumber="26">23</td>
<td id="DA66054F77040018FC68AA5893234868" box="[941,1074,779,807]" gridcol="5" gridrow="10" pageId="13" pageNumber="26">1.63</td>
<td id="DA66054F77040018FB83AA5893804868" box="[1094,1169,779,807]" gridcol="6" gridrow="10" pageId="13" pageNumber="26">0.97</td>
<td id="DA66054F77040018FB60AA5892184868" box="[1189,1289,779,807]" gridcol="7" gridrow="10" pageId="13" pageNumber="26">190</td>
<td id="DA66054F77040018FAEAAA5892654868" box="[1327,1396,779,807]" gridcol="8" gridrow="10" pageId="13" pageNumber="26">113</td>
</tr>
<tr id="99B76C3377040018FF7CAA6092654800" box="[185,1396,819,847]" gridrow="11" pageId="13" pageNumber="26" rowspan-0="1" rowspan-1="1">
<td id="DA66054F77040018FDD7AA6095B24800" box="[530,675,819,847]" gridcol="2" gridrow="11" pageId="13" pageNumber="26">29</td>
<td id="DA66054F77040018FD7FAA6094514800" box="[698,832,819,847]" gridcol="3" gridrow="11" pageId="13" pageNumber="26">
9.29×10 
<superScript id="D0F2C3397704FFEAFCEBAA7E94294870" attach="right" box="[814,824,813,831]" fontSize="7" pageId="13" pageNumber="26">4</superScript>
</td>
<td id="DA66054F77040018FC93AA60948A4800" box="[854,923,819,847]" gridcol="4" gridrow="11" pageId="13" pageNumber="26">15</td>
<td id="DA66054F77040018FC68AA6093234800" box="[941,1074,819,847]" gridcol="5" gridrow="11" pageId="13" pageNumber="26">1.87</td>
<td id="DA66054F77040018FB83AA6093804800" box="[1094,1169,819,847]" gridcol="6" gridrow="11" pageId="13" pageNumber="26">0.89</td>
<td id="DA66054F77040018FB60AA6092184800" box="[1189,1289,819,847]" gridcol="7" gridrow="11" pageId="13" pageNumber="26">198</td>
<td id="DA66054F77040018FAEAAA6092654800" box="[1327,1396,819,847]" gridcol="8" gridrow="11" pageId="13" pageNumber="26">95</td>
</tr>
<tr id="99B76C3377040018FF7CAA0892654838" box="[185,1396,859,887]" gridrow="12" pageId="13" pageNumber="26" rowspan-0="1" rowspan-1="1">
<td id="DA66054F77040018FDD7AA0895B24838" box="[530,675,859,887]" gridcol="2" gridrow="12" pageId="13" pageNumber="26">30</td>
<td id="DA66054F77040018FD7FAA0894514838" box="[698,832,859,887]" gridcol="3" gridrow="12" pageId="13" pageNumber="26">
4.45×10 
<superScript id="D0F2C3397704FFEAFCEBAA0694294828" attach="right" box="[814,824,853,871]" fontSize="7" pageId="13" pageNumber="26">4</superScript>
</td>
<td id="DA66054F77040018FC93AA08948A4838" box="[854,923,859,887]" gridcol="4" gridrow="12" pageId="13" pageNumber="26">18</td>
<td id="DA66054F77040018FC68AA0893234838" box="[941,1074,859,887]" gridcol="5" gridrow="12" pageId="13" pageNumber="26">2.12</td>
<td id="DA66054F77040018FB83AA0893804838" box="[1094,1169,859,887]" gridcol="6" gridrow="12" pageId="13" pageNumber="26">1.14</td>
<td id="DA66054F77040018FB60AA0892184838" box="[1189,1289,859,887]" gridcol="7" gridrow="12" pageId="13" pageNumber="26">116</td>
<td id="DA66054F77040018FAEAAA0892654838" box="[1327,1396,859,887]" gridcol="8" gridrow="12" pageId="13" pageNumber="26">62</td>
</tr>
<tr id="99B76C3377040018FF7CAAD0926548D0" box="[185,1396,899,927]" gridrow="13" pageId="13" pageNumber="26" rowspan-0="1" rowspan-1="1">
<td id="DA66054F77040018FDD7AAD095B248D0" box="[530,675,899,927]" gridcol="2" gridrow="13" pageId="13" pageNumber="26">31</td>
<td id="DA66054F77040018FD7FAAD0945148D0" box="[698,832,899,927]" gridcol="3" gridrow="13" pageId="13" pageNumber="26">
3.93×10 
<superScript id="D0F2C3397704FFEAFCEBAA2E942948C0" attach="right" box="[814,824,893,911]" fontSize="7" pageId="13" pageNumber="26">4</superScript>
</td>
<td id="DA66054F77040018FC93AAD0948A48D0" box="[854,923,899,927]" gridcol="4" gridrow="13" pageId="13" pageNumber="26">13</td>
<td id="DA66054F77040018FC68AAD0932348D0" box="[941,1074,899,927]" gridcol="5" gridrow="13" pageId="13" pageNumber="26">2.21</td>
<td id="DA66054F77040018FB83AAD0938048D0" box="[1094,1169,899,927]" gridcol="6" gridrow="13" pageId="13" pageNumber="26">0.98</td>
<td id="DA66054F77040018FB60AAD0921848D0" box="[1189,1289,899,927]" gridcol="7" gridrow="13" pageId="13" pageNumber="26">111</td>
<td id="DA66054F77040018FAEAAAD0926548D0" box="[1327,1396,899,927]" gridcol="8" gridrow="13" pageId="13" pageNumber="26">49</td>
</tr>
<tr id="99B76C3377040018FF7CAAF892654888" box="[185,1396,939,967]" gridrow="14" pageId="13" pageNumber="26" rowspan-0="1" rowspan-1="1">
<td id="DA66054F77040018FDD7AAF895B24888" box="[530,675,939,967]" gridcol="2" gridrow="14" pageId="13" pageNumber="26">32</td>
<td id="DA66054F77040018FD7FAAF894514888" box="[698,832,939,967]" gridcol="3" gridrow="14" pageId="13" pageNumber="26">
3.52×10 
<superScript id="D0F2C3397704FFEAFCEBAAF6942948F8" attach="right" box="[814,824,933,951]" fontSize="7" pageId="13" pageNumber="26">4</superScript>
</td>
<td id="DA66054F77040018FC93AAF8948A4888" box="[854,923,939,967]" gridcol="4" gridrow="14" pageId="13" pageNumber="26">23</td>
<td id="DA66054F77040018FC68AAF893234888" box="[941,1074,939,967]" gridcol="5" gridrow="14" pageId="13" pageNumber="26">1.97</td>
<td id="DA66054F77040018FB83AAF893804888" box="[1094,1169,939,967]" gridcol="6" gridrow="14" pageId="13" pageNumber="26">1.25</td>
<td id="DA66054F77040018FB60AAF892184888" box="[1189,1289,939,967]" gridcol="7" gridrow="14" pageId="13" pageNumber="26">96</td>
<tr id="99B76C3377040018FF7CAA80926548A0" box="[185,1396,979,1007]" gridrow="15" pageId="13" pageNumber="26" rowspan-0="1" rowspan-1="1">
<td id="DA66054F77040018FAEAAAF892654888" box="[1327,1396,939,967]" gridcol="8" gridrow="14" pageId="13" pageNumber="26">
<td id="DA66054F77040018FDD7AA8095B248A0" box="[530,675,979,1007]" gridcol="2" gridrow="15" pageId="13" pageNumber="26">6033</td>
</td>
<td id="DA66054F77040018FD7FAA80945148A0" box="[698,832,979,1007]" gridcol="3" gridrow="15" pageId="13" pageNumber="26">
2.93×10 
<superScript id="D0F2C3397704FFEAFCEBAA9E94294890" attach="right" box="[814,824,973,991]" fontSize="7" pageId="13" pageNumber="26">4</superScript>
</td>
<td id="DA66054F77040018FC93AA80948A48A0" box="[854,923,979,1007]" gridcol="4" gridrow="15" pageId="13" pageNumber="26">29</td>
<td id="DA66054F77040018FC68AA80932348A0" box="[941,1074,979,1007]" gridcol="5" gridrow="15" pageId="13" pageNumber="26">2.14</td>
<td id="DA66054F77040018FB83AA80938048A0" box="[1094,1169,979,1007]" gridcol="6" gridrow="15" pageId="13" pageNumber="26">1.25</td>
<td id="DA66054F77040018FB60AA80921848A0" box="[1189,1289,979,1007]" gridcol="7" gridrow="15" pageId="13" pageNumber="26">93</td>
<td id="DA66054F77040018FAEAAA80926548A0" box="[1327,1396,979,1007]" gridcol="8" gridrow="15" pageId="13" pageNumber="26">54</td>
</tr>
</tr>
<tr id="99B76C3377040018FF7CAAA892654F58" box="[185,1396,1019,1047]" gridrow="16" pageId="13" pageNumber="26" rowspan-0="1" rowspan-1="1" rowspan-3="1">
<td id="DA66054F77040018FDD7AAA895B24F58" box="[530,675,1019,1047]" gridcol="2" gridrow="16" pageId="13" pageNumber="26">average</td>
<td id="DA66054F77040018FC93AAA8948A4F58" box="[854,923,1019,1047]" gridcol="4" gridrow="16" pageId="13" pageNumber="26">125</td>
<td id="DA66054F77040018FC68AAA893234F58" box="[941,1074,1019,1047]" gridcol="5" gridrow="16" pageId="13" pageNumber="26">1.96</td>
<td id="DA66054F77040018FB83AAA893804F58" box="[1094,1169,1019,1047]" gridcol="6" gridrow="16" pageId="13" pageNumber="26">1.08</td>
<td id="DA66054F77040018FB60AAA892184F58" box="[1189,1289,1019,1047]" gridcol="7" gridrow="16" pageId="13" pageNumber="26">132</td>
<td id="DA66054F77040018FAEAAAA892654F58" box="[1327,1396,1019,1047]" gridcol="8" gridrow="16" pageId="13" pageNumber="26">
<emphasis id="15F3B2637704FFEAFAF2AAA892494F58" box="[1335,1368,1019,1047]" italics="true" pageId="13" pageNumber="26">72</emphasis>
</td>
</tr>
<tr id="99B76C3377040018FF7CAD1892654F28" box="[185,1396,1099,1127]" gridrow="17" pageId="13" pageNumber="26">
<th id="DA66054F77040018FF7CAD1696454F28" box="[185,340,1093,1127]" gridcol="0" gridrow="17" pageId="13" pageNumber="26">La Preciosa</th>
<td id="DA66054F77040018FEAFAD1696EF4F28" box="[362,510,1093,1127]" gridcol="1" gridrow="17" pageId="13" pageNumber="26">
<date id="533948B17704FFEAFEAFAD1896EC4F28" box="[362,509,1099,1127]" pageId="13" pageNumber="26" value="2011-10-26">26/10/2011</date>
</td>
<td id="DA66054F77040018FDD7AD1695B24F28" box="[530,675,1093,1127]" gridcol="2" gridrow="17" pageId="13" pageNumber="26">26</td>
<td id="DA66054F77040018FD7FAD1694514F28" box="[698,832,1093,1127]" gridcol="3" gridrow="17" pageId="13" pageNumber="26">
4.36×10 
<superScript id="D0F2C3397704FFEAFCEBAD1694294F18" attach="right" box="[814,824,1093,1111]" fontSize="7" pageId="13" pageNumber="26">5</superScript>
</td>
<td id="DA66054F77040018FC93AD16948A4F28" box="[854,923,1093,1127]" gridcol="4" gridrow="17" pageId="13" pageNumber="26">21</td>
<td id="DA66054F77040018FC68AD1693234F28" box="[941,1074,1093,1127]" gridcol="5" gridrow="17" pageId="13" pageNumber="26">1.18</td>
<td id="DA66054F77040018FB83AD1693804F28" box="[1094,1169,1093,1127]" gridcol="6" gridrow="17" pageId="13" pageNumber="26">0.82</td>
<td id="DA66054F77040018FB60AD1692184F28" box="[1189,1289,1093,1127]" gridcol="7" gridrow="17" pageId="13" pageNumber="26">595</td>
<td id="DA66054F77040018FAEAAD1692654F28" box="[1327,1396,1093,1127]" gridcol="8" gridrow="17" pageId="13" pageNumber="26">414</td>
</tr>
<tr id="99B76C3377040018FF7CAD2092654FC0" box="[185,1396,1139,1167]" gridrow="18" pageId="13" pageNumber="26" rowspan-0="1" rowspan-1="1">
<td id="DA66054F77040018FDD7AD2095B24FC0" box="[530,675,1139,1167]" gridcol="2" gridrow="18" pageId="13" pageNumber="26">27</td>
<td id="DA66054F77040018FD7FAD2094514FC0" box="[698,832,1139,1167]" gridcol="3" gridrow="18" pageId="13" pageNumber="26">
1.53×10 
<superScript id="D0F2C3397704FFEAFCEBAD3E94294F30" attach="right" box="[814,824,1133,1151]" fontSize="7" pageId="13" pageNumber="26">5</superScript>
</td>
<td id="DA66054F77040018FC93AD20948A4FC0" box="[854,923,1139,1167]" gridcol="4" gridrow="18" pageId="13" pageNumber="26">14</td>
<td id="DA66054F77040018FC68AD2093234FC0" box="[941,1074,1139,1167]" gridcol="5" gridrow="18" pageId="13" pageNumber="26">1.50</td>
<td id="DA66054F77040018FB83AD2093804FC0" box="[1094,1169,1139,1167]" gridcol="6" gridrow="18" pageId="13" pageNumber="26">1.46</td>
<td id="DA66054F77040018FB60AD2092184FC0" box="[1189,1289,1139,1167]" gridcol="7" gridrow="18" pageId="13" pageNumber="26">334</td>
<td id="DA66054F77040018FAEAAD2092654FC0" box="[1327,1396,1139,1167]" gridcol="8" gridrow="18" pageId="13" pageNumber="26">327</td>
</tr>
<tr id="99B76C3377040018FF7CADC892654FF8" box="[185,1396,1179,1207]" gridrow="19" pageId="13" pageNumber="26" rowspan-0="1" rowspan-1="1" rowspan-6="1" rowspan-8="1">
<td id="DA66054F77040018FDD7ADC895B24FF8" box="[530,675,1179,1207]" gridcol="2" gridrow="19" pageId="13" pageNumber="26">28</td>
<td id="DA66054F77040018FD7FADC894514FF8" box="[698,832,1179,1207]" gridcol="3" gridrow="19" pageId="13" pageNumber="26">
8.44×10 
<superScript id="D0F2C3397704FFEAFCEBADC694294FE8" attach="right" box="[814,824,1173,1191]" fontSize="7" pageId="13" pageNumber="26">4</superScript>
</td>
<td id="DA66054F77040018FC93ADC8948A4FF8" box="[854,923,1179,1207]" gridcol="4" gridrow="19" pageId="13" pageNumber="26">1</td>
<td id="DA66054F77040018FC68ADC893234FF8" box="[941,1074,1179,1207]" gridcol="5" gridrow="19" pageId="13" pageNumber="26">2.84</td>
<td id="DA66054F77040018FB60ADC892184FF8" box="[1189,1289,1179,1207]" gridcol="7" gridrow="19" pageId="13" pageNumber="26">384</td>
</tr>
<tr id="99B76C3377040018FF7CAD9092654F90" box="[185,1396,1219,1247]" gridrow="20" pageId="13" pageNumber="26" rowspan-0="1" rowspan-1="1" rowspan-3="1">
<td id="DA66054F77040018FDD7AD9095B24F90" box="[530,675,1219,1247]" gridcol="2" gridrow="20" pageId="13" pageNumber="26">average</td>
<td id="DA66054F77040018FC93AD90948A4F90" box="[854,923,1219,1247]" gridcol="4" gridrow="20" pageId="13" pageNumber="26">36</td>
<td id="DA66054F77040018FC68AD9093234F90" box="[941,1074,1219,1247]" gridcol="5" gridrow="20" pageId="13" pageNumber="26">1.35</td>
<td id="DA66054F77040018FB83AD9093804F90" box="[1094,1169,1219,1247]" gridcol="6" gridrow="20" pageId="13" pageNumber="26">1045</td>
<td id="DA66054F77040018FB60AD9092184F90" box="[1189,1289,1219,1247]" gridcol="7" gridrow="20" pageId="13" pageNumber="26">488</td>
<td id="DA66054F77040018FAEAAD9092654F90" box="[1327,1396,1219,1247]" gridcol="8" gridrow="20" pageId="13" pageNumber="26">
<emphasis id="15F3B2637704FFEAFAEAAD9092714F90" box="[1327,1376,1219,1247]" italics="true" pageId="13" pageNumber="26">369</emphasis>
</td>
</tr>
<tr id="99B76C3377040018FF7CAC4092654E60" box="[185,1396,1299,1327]" gridrow="21" pageId="13" pageNumber="26">
<th id="DA66054F77040018FF7CAC5E96454E60" box="[185,340,1293,1327]" gridcol="0" gridrow="21" pageId="13" pageNumber="26">La Preciosa</th>
<td id="DA66054F77040018FEAFAC5E96EF4E60" box="[362,510,1293,1327]" gridcol="1" gridrow="21" pageId="13" pageNumber="26">
<date id="533948B17704FFEAFEAFAC4096EC4E60" box="[362,509,1299,1327]" pageId="13" pageNumber="26" value="2012-09-12">12/09/2012</date>
</td>
<td id="DA66054F77040018FDD7AC5E95B24E60" box="[530,675,1293,1327]" gridcol="2" gridrow="21" pageId="13" pageNumber="26">19</td>
<td id="DA66054F77040018FD7FAC5E94514E60" box="[698,832,1293,1327]" gridcol="3" gridrow="21" pageId="13" pageNumber="26">
2.06×10 
<superScript id="D0F2C3397704FFEAFCEBAC5E94294E50" attach="right" box="[814,824,1293,1311]" fontSize="7" pageId="13" pageNumber="26">5</superScript>
</td>
<td id="DA66054F77040018FC93AC5E948A4E60" box="[854,923,1293,1327]" gridcol="4" gridrow="21" pageId="13" pageNumber="26">96</td>
<td id="DA66054F77040018FC68AC5E93234E60" box="[941,1074,1293,1327]" gridcol="5" gridrow="21" pageId="13" pageNumber="26">1.40</td>
<td id="DA66054F77040018FB83AC5E93804E60" box="[1094,1169,1293,1327]" gridcol="6" gridrow="21" pageId="13" pageNumber="26">0.65</td>
<td id="DA66054F77040018FB60AC5E92184E60" box="[1189,1289,1293,1327]" gridcol="7" gridrow="21" pageId="13" pageNumber="26">307</td>
<td id="DA66054F77040018FAEAAC5E92654E60" box="[1327,1396,1293,1327]" gridcol="8" gridrow="21" pageId="13" pageNumber="26">141</td>
</tr>
<tr id="99B76C3377040018FF7CAC6892654E18" box="[185,1396,1339,1367]" gridrow="22" pageId="13" pageNumber="26" rowspan-0="1" rowspan-1="1">
<td id="DA66054F77040018FDD7AC6895B24E18" box="[530,675,1339,1367]" gridcol="2" gridrow="22" pageId="13" pageNumber="26">20</td>
<td id="DA66054F77040018FD7FAC6894514E18" box="[698,832,1339,1367]" gridcol="3" gridrow="22" pageId="13" pageNumber="26">
1.40×10 
<superScript id="D0F2C3397704FFEAFCEBAC6694294E08" attach="right" box="[814,824,1333,1351]" fontSize="7" pageId="13" pageNumber="26">5</superScript>
</td>
<td id="DA66054F77040018FC93AC68948A4E18" box="[854,923,1339,1367]" gridcol="4" gridrow="22" pageId="13" pageNumber="26">72</td>
<td id="DA66054F77040018FC68AC6893234E18" box="[941,1074,1339,1367]" gridcol="5" gridrow="22" pageId="13" pageNumber="26">2.13</td>
<td id="DA66054F77040018FB83AC6893804E18" box="[1094,1169,1339,1367]" gridcol="6" gridrow="22" pageId="13" pageNumber="26">1.03</td>
<td id="DA66054F77040018FB60AC6892184E18" box="[1189,1289,1339,1367]" gridcol="7" gridrow="22" pageId="13" pageNumber="26">326</td>
<td id="DA66054F77040018FAEAAC6892654E18" box="[1327,1396,1339,1367]" gridcol="8" gridrow="22" pageId="13" pageNumber="26">158</td>
</tr>
<tr id="99B76C3377040018FF7CAC3092654E30" box="[185,1396,1379,1407]" gridrow="23" pageId="13" pageNumber="26" rowspan-0="1" rowspan-1="1">
<td id="DA66054F77040018FDD7AC3095B24E30" box="[530,675,1379,1407]" gridcol="2" gridrow="23" pageId="13" pageNumber="26">21</td>
<td id="DA66054F77040018FD7FAC3094514E30" box="[698,832,1379,1407]" gridcol="3" gridrow="23" pageId="13" pageNumber="26">
1.23×10 
<superScript id="D0F2C3397704FFEAFCEBAC0E94294E20" attach="right" box="[814,824,1373,1391]" fontSize="7" pageId="13" pageNumber="26">5</superScript>
</td>
<td id="DA66054F77040018FC93AC30948A4E30" box="[854,923,1379,1407]" gridcol="4" gridrow="23" pageId="13" pageNumber="26">50</td>
<td id="DA66054F77040018FC68AC3093234E30" box="[941,1074,1379,1407]" gridcol="5" gridrow="23" pageId="13" pageNumber="26">3.65</td>
<td id="DA66054F77040018FB83AC3093804E30" box="[1094,1169,1379,1407]" gridcol="6" gridrow="23" pageId="13" pageNumber="26">0.94</td>
<td id="DA66054F77040018FB60AC3092184E30" box="[1189,1289,1379,1407]" gridcol="7" gridrow="23" pageId="13" pageNumber="26">490</td>
<td id="DA66054F77040018FAEAAC3092654E30" box="[1327,1396,1379,1407]" gridcol="8" gridrow="23" pageId="13" pageNumber="26">127</td>
</tr>
<tr id="99B76C3377040018FF7CACD892654EE8" box="[185,1396,1419,1447]" gridrow="24" pageId="13" pageNumber="26" rowspan-0="1" rowspan-1="1">
<td id="DA66054F77040018FDD7ACD895B24EE8" box="[530,675,1419,1447]" gridcol="2" gridrow="24" pageId="13" pageNumber="26">22</td>
<td id="DA66054F77040018FD7FACD894514EE8" box="[698,832,1419,1447]" gridcol="3" gridrow="24" pageId="13" pageNumber="26">
6.09×10 
<superScript id="D0F2C3397704FFEAFCEBACD694294ED8" attach="right" box="[814,824,1413,1431]" fontSize="7" pageId="13" pageNumber="26">4</superScript>
</td>
<td id="DA66054F77040018FC93ACD8948A4EE8" box="[854,923,1419,1447]" gridcol="4" gridrow="24" pageId="13" pageNumber="26">20</td>
<td id="DA66054F77040018FC68ACD893234EE8" box="[941,1074,1419,1447]" gridcol="5" gridrow="24" pageId="13" pageNumber="26">2.19</td>
<td id="DA66054F77040018FB83ACD893804EE8" box="[1094,1169,1419,1447]" gridcol="6" gridrow="24" pageId="13" pageNumber="26">0.75</td>
<td id="DA66054F77040018FB60ACD892184EE8" box="[1189,1289,1419,1447]" gridcol="7" gridrow="24" pageId="13" pageNumber="26">162</td>
<td id="DA66054F77040018FAEAACD892654EE8" box="[1327,1396,1419,1447]" gridcol="8" gridrow="24" pageId="13" pageNumber="26">56</td>
</tr>
<tr id="99B76C3377040018FF7CACE092654E80" box="[185,1396,1459,1487]" gridrow="25" pageId="13" pageNumber="26" rowspan-0="1" rowspan-1="1">
<td id="DA66054F77040018FDD7ACE095B24E80" box="[530,675,1459,1487]" gridcol="2" gridrow="25" pageId="13" pageNumber="26">23</td>
<td id="DA66054F77040018FD7FACE094514E80" box="[698,832,1459,1487]" gridcol="3" gridrow="25" pageId="13" pageNumber="26">
2.47×10 
<superScript id="D0F2C3397704FFEAFCEBACFE94294EF0" attach="right" box="[814,824,1453,1471]" fontSize="7" pageId="13" pageNumber="26">4</superScript>
</td>
<td id="DA66054F77040018FC93ACE0948A4E80" box="[854,923,1459,1487]" gridcol="4" gridrow="25" pageId="13" pageNumber="26">4</td>
<td id="DA66054F77040018FC68ACE093234E80" box="[941,1074,1459,1487]" gridcol="5" gridrow="25" pageId="13" pageNumber="26">1.74</td>
<td id="DA66054F77040018FB83ACE093804E80" box="[1094,1169,1459,1487]" gridcol="6" gridrow="25" pageId="13" pageNumber="26">1.72</td>
<td id="DA66054F77040018FB60ACE092184E80" box="[1189,1289,1459,1487]" gridcol="7" gridrow="25" pageId="13" pageNumber="26">60</td>
<td id="DA66054F77040018FAEAACE092654E80" box="[1327,1396,1459,1487]" gridcol="8" gridrow="25" pageId="13" pageNumber="26">60</td>
</tr>
<tr id="99B76C3377040018FF7CAC8892654EB8" box="[185,1396,1499,1527]" gridrow="26" pageId="13" pageNumber="26" rowspan-0="1" rowspan-1="1" rowspan-3="1">
<td id="DA66054F77040018FDD7AC8895B24EB8" box="[530,675,1499,1527]" gridcol="2" gridrow="26" pageId="13" pageNumber="26">average</td>
<td id="DA66054F77040018FC93AC88948A4EB8" box="[854,923,1499,1527]" gridcol="4" gridrow="26" pageId="13" pageNumber="26">242</td>
<td id="DA66054F77040018FC68AC8893234EB8" box="[941,1074,1499,1527]" gridcol="5" gridrow="26" pageId="13" pageNumber="26">2.15</td>
<td id="DA66054F77040018FB83AC8893804EB8" box="[1094,1169,1499,1527]" gridcol="6" gridrow="26" pageId="13" pageNumber="26">0.85</td>
<td id="DA66054F77040018FB60AC8892184EB8" box="[1189,1289,1499,1527]" gridcol="7" gridrow="26" pageId="13" pageNumber="26">334</td>
<td id="DA66054F77040018FAEAAC8892654EB8" box="[1327,1396,1499,1527]" gridcol="8" gridrow="26" pageId="13" pageNumber="26">
<emphasis id="15F3B2637704FFEAFAEAAC8892714EB8" box="[1327,1376,1499,1527]" italics="true" pageId="13" pageNumber="26">135</emphasis>
</td>
</tr>
<tr id="99B76C3377040018FF7CAF7892654D08" box="[185,1396,1579,1607]" gridrow="27" pageId="13" pageNumber="26">
<th id="DA66054F77040018FF7CAF7696454D08" box="[185,340,1573,1607]" gridcol="0" gridrow="27" pageId="13" pageNumber="26">La Preciosa</th>
<td id="DA66054F77040018FEAFAF7696EF4D08" box="[362,510,1573,1607]" gridcol="1" gridrow="27" pageId="13" pageNumber="26">
<date id="533948B17704FFEAFEAFAF7896EC4D08" box="[362,509,1579,1607]" pageId="13" pageNumber="26" value="2012-10-25">25/10/2012</date>
</td>
<td id="DA66054F77040018FDD7AF7695B24D08" box="[530,675,1573,1607]" gridcol="2" gridrow="27" pageId="13" pageNumber="26">23</td>
<td id="DA66054F77040018FD7FAF7694514D08" box="[698,832,1573,1607]" gridcol="3" gridrow="27" pageId="13" pageNumber="26">
1.24×10 
<superScript id="D0F2C3397704FFEAFCEBAF7694294D78" attach="right" box="[814,824,1573,1591]" fontSize="7" pageId="13" pageNumber="26">5</superScript>
</td>
<td id="DA66054F77040018FC93AF76948A4D08" box="[854,923,1573,1607]" gridcol="4" gridrow="27" pageId="13" pageNumber="26">3</td>
<td id="DA66054F77040018FC68AF7693234D08" box="[941,1074,1573,1607]" gridcol="5" gridrow="27" pageId="13" pageNumber="26">1.24</td>
<td id="DA66054F77040018FB83AF7693804D08" box="[1094,1169,1573,1607]" gridcol="6" gridrow="27" pageId="13" pageNumber="26">0.51</td>
<td id="DA66054F77040018FB60AF7692184D08" box="[1189,1289,1573,1607]" gridcol="7" gridrow="27" pageId="13" pageNumber="26">192</td>
<td id="DA66054F77040018FAEAAF7692654D08" box="[1327,1396,1573,1607]" gridcol="8" gridrow="27" pageId="13" pageNumber="26">79</td>
</tr>
<tr id="99B76C3377040018FF7CAF0092654D20" box="[185,1396,1619,1647]" gridrow="28" pageId="13" pageNumber="26" rowspan-0="1" rowspan-1="1">
<td id="DA66054F77040018FDD7AF0095B24D20" box="[530,675,1619,1647]" gridcol="2" gridrow="28" pageId="13" pageNumber="26">24</td>
<td id="DA66054F77040018FD7FAF0094514D20" box="[698,832,1619,1647]" gridcol="3" gridrow="28" pageId="13" pageNumber="26">
2.09×10 
<superScript id="D0F2C3397704FFEAFCEBAF1E94294D10" attach="right" box="[814,824,1613,1631]" fontSize="7" pageId="13" pageNumber="26">4</superScript>
</td>
<td id="DA66054F77040018FC93AF00948A4D20" box="[854,923,1619,1647]" gridcol="4" gridrow="28" pageId="13" pageNumber="26">17</td>
<td id="DA66054F77040018FC68AF0093234D20" box="[941,1074,1619,1647]" gridcol="5" gridrow="28" pageId="13" pageNumber="26">1.11</td>
<td id="DA66054F77040018FB83AF0093804D20" box="[1094,1169,1619,1647]" gridcol="6" gridrow="28" pageId="13" pageNumber="26">0.30</td>
<td id="DA66054F77040018FB60AF0092184D20" box="[1189,1289,1619,1647]" gridcol="7" gridrow="28" pageId="13" pageNumber="26">57</td>
<td id="DA66054F77040018FAEAAF0092654D20" box="[1327,1396,1619,1647]" gridcol="8" gridrow="28" pageId="13" pageNumber="26">15</td>
</tr>
<tr id="99B76C3377040018FF7CAF2892654DD8" box="[185,1396,1659,1687]" gridrow="29" pageId="13" pageNumber="26" rowspan-0="1" rowspan-1="1" rowspan-6="1" rowspan-8="1">
<td id="DA66054F77040018FDD7AF2895B24DD8" box="[530,675,1659,1687]" gridcol="2" gridrow="29" pageId="13" pageNumber="26">25</td>
<td id="DA66054F77040018FD7FAF2894514DD8" box="[698,832,1659,1687]" gridcol="3" gridrow="29" pageId="13" pageNumber="26">
4.61×10 
<superScript id="D0F2C3397704FFEAFCEBAF2694294DC8" attach="right" box="[814,824,1653,1671]" fontSize="7" pageId="13" pageNumber="26">4</superScript>
</td>
<td id="DA66054F77040018FC93AF28948A4DD8" box="[854,923,1659,1687]" gridcol="4" gridrow="29" pageId="13" pageNumber="26">1</td>
<td id="DA66054F77040018FC68AF2893234DD8" box="[941,1074,1659,1687]" gridcol="5" gridrow="29" pageId="13" pageNumber="26">0.48</td>
<td id="DA66054F77040018FB60AF2892184DD8" box="[1189,1289,1659,1687]" gridcol="7" gridrow="29" pageId="13" pageNumber="26">43</td>
</tr>
<tr id="99B76C3377040018FF7CAFF092654DF0" box="[185,1396,1699,1727]" gridrow="30" pageId="13" pageNumber="26" rowspan-0="1" rowspan-1="1" rowspan-3="1">
<td id="DA66054F77040018FDD7AFF095B24DF0" box="[530,675,1699,1727]" gridcol="2" gridrow="30" pageId="13" pageNumber="26">average</td>
<td id="DA66054F77040018FC93AFF0948A4DF0" box="[854,923,1699,1727]" gridcol="4" gridrow="30" pageId="13" pageNumber="26">21</td>
<td id="DA66054F77040018FC68AFF093234DF0" box="[941,1074,1699,1727]" gridcol="5" gridrow="30" pageId="13" pageNumber="26">1.09</td>
<td id="DA66054F77040018FB83AFF093804DF0" box="[1094,1169,1699,1727]" gridcol="6" gridrow="30" pageId="13" pageNumber="26">0.31</td>
<td id="DA66054F77040018FB60AFF092184DF0" box="[1189,1289,1699,1727]" gridcol="7" gridrow="30" pageId="13" pageNumber="26">76</td>
<td id="DA66054F77040018FAEAAFF092654DF0" box="[1327,1396,1699,1727]" gridcol="8" gridrow="30" pageId="13" pageNumber="26">
<emphasis id="15F3B2637704FFEAFAF2AFF092494DF0" box="[1335,1368,1699,1727]" italics="true" pageId="13" pageNumber="26">24</emphasis>
</td>
</tr>
<tr id="99B76C3377040018FF7CAFBE92654C40" box="[185,1396,1773,1807]" gridrow="31" pageId="13" pageNumber="26">
<th id="DA66054F77040018FF7CAFBE96454C40" box="[185,340,1773,1807]" gridcol="0" gridrow="31" pageId="13" pageNumber="26">La Cruz</th>
<td id="DA66054F77040018FEAFAFBE96EF4C40" box="[362,510,1773,1807]" gridcol="1" gridrow="31" pageId="13" pageNumber="26">
<date id="533948B17704FFEAFEAFAFA096EC4C40" box="[362,509,1779,1807]" pageId="13" pageNumber="26" value="2010-06-25">25/06/2010</date>
</td>
<td id="DA66054F77040018FDD7AFBE95B24C40" box="[530,675,1773,1807]" gridcol="2" gridrow="31" pageId="13" pageNumber="26">experiment</td>
<td id="DA66054F77040018FD7FAFBE94514C40" box="[698,832,1773,1807]" gridcol="3" gridrow="31" pageId="13" pageNumber="26">
3.50×10 
<superScript id="D0F2C3397704FFEAFCEBAFBE94294DB0" attach="right" box="[814,824,1773,1791]" fontSize="7" pageId="13" pageNumber="26">5</superScript>
</td>
<td id="DA66054F77040018FC93AFBE948A4C40" box="[854,923,1773,1807]" gridcol="4" gridrow="31" pageId="13" pageNumber="26">40</td>
<td id="DA66054F77040018FC68AFBE93234C40" box="[941,1074,1773,1807]" gridcol="5" gridrow="31" pageId="13" pageNumber="26">0.275</td>
<td id="DA66054F77040018FB83AFBE93804C40" box="[1094,1169,1773,1807]" gridcol="6" gridrow="31" pageId="13" pageNumber="26">0.065</td>
<td id="DA66054F77040018FB60AFBE92184C40" box="[1189,1289,1773,1807]" gridcol="7" gridrow="31" pageId="13" pageNumber="26">115</td>
<td id="DA66054F77040018FAEAAFBE92654C40" box="[1327,1396,1773,1807]" gridcol="8" gridrow="31" pageId="13" pageNumber="26">
<emphasis id="15F3B2637704FFEAFAF2AFA092494C40" box="[1335,1368,1779,1807]" italics="true" pageId="13" pageNumber="26">27</emphasis>
</td>
</tr>
</table>
</paragraph>
<caption id="73F83EF97704FFEAFFB8AE31928A4CDB" ID-Table-UUID="73F83EF97704FFEAFFB8AE31928A4CDB" httpUri="http://table.plazi.org/id/73F83EF97704FFEAFFB8AE31928A4CDB" pageId="13" pageNumber="26" startId="13.[125,181,1890,1911]" targetBox="[185,1396,262,1807]" targetIsTable="true" targetPageId="13" targetType="table">
<paragraph id="27386E717704FFEAFFB8AE31928A4CDB" blockId="13.[125,1453,1889,1940]" pageId="13" pageNumber="26">
<emphasis id="15F3B2637704FFEAFFB8AE3197DF4C38" bold="true" box="[125,206,1890,1911]" pageId="13" pageNumber="26">Table 1.</emphasis>
Feeding experiments with 
<taxonomicName id="E08715F27704FFEAFE16AE3195084C37" box="[467,537,1890,1912]" class="Heterotrichea" family="Spirostomidae" genus="Spirostomum" kingdom="Chromista" order="Heterotrichida" pageId="13" pageNumber="26" phylum="Ciliophora" rank="species" species="teres">
<emphasis id="15F3B2637704FFEAFE16AE3195084C37" box="[467,537,1890,1912]" italics="true" pageId="13" pageNumber="26">S. teres</emphasis>
</taxonomicName>
(APP = autotrophic picoplankton [cells mL 
<superScript id="D0F2C3397704FFEAFC7BAE3294DD4C22" attach="left" box="[958,972,1889,1901]" fontSize="5" pageId="13" pageNumber="26">–1</superScript>
]: N = number of inspected ciliate cells; F = clearance rate [nL cell 
<superScript id="D0F2C3397704FFEAFEE1AE2E96234CC6" attach="both" box="[292,306,1917,1929]" fontSize="5" pageId="13" pageNumber="26">–1</superScript>
h 
<superScript id="D0F2C3397704FFEAFEFBAE2E965D4CC6" attach="left" box="[318,332,1917,1929]" fontSize="5" pageId="13" pageNumber="26">–1</superScript>
]; G = grazing rate [cells cell 
<superScript id="D0F2C3397704FFEAFDA5AE2E957F4CC6" attach="both" box="[608,622,1917,1929]" fontSize="5" pageId="13" pageNumber="26">–1</superScript>
h 
<superScript id="D0F2C3397704FFEAFDBFAE2E95994CC6" attach="left" box="[634,648,1917,1929]" fontSize="5" pageId="13" pageNumber="26">–1</superScript>
]; average was calculated as weighted by analysed ciliate cells in different layers).
</paragraph>
</caption>
<caption id="73F83EF97707FFE9FF41AF7B94DB4D15" ID-DOI="http://doi.org/10.5281/zenodo.10994495" ID-Zenodo-Dep="10994495" httpUri="https://zenodo.org/record/10994495/files/figure.png" pageId="14" pageNumber="27" startId="14.[132,167,1576,1598]" targetBox="[165,1415,245,1540]" targetPageId="14" targetType="figure">
<paragraph id="27386E717707FFE9FF41AF7B94DB4D15" blockId="14.[132,1463,1576,1626]" pageId="14" pageNumber="27">
<emphasis id="15F3B2637707FFE9FF41AF7B97C14D72" bold="true" box="[132,208,1576,1598]" pageId="14" pageNumber="27">Fig. 10.</emphasis>
Feeding experiments with FLB in Lake Alchichica 
<emphasis id="15F3B2637707FFE9FD05AF7B95E44D71" bold="true" box="[704,757,1576,1598]" pageId="14" pageNumber="27">(a, b)</emphasis>
, La Preciosa (
<emphasis id="15F3B2637707FFE9FCBAAF7A949B4D71" bold="true" box="[895,906,1577,1598]" pageId="14" pageNumber="27">c</emphasis>
, 
<emphasis id="15F3B2637707FFE9FC50AF7A94B34D71" bold="true" box="[917,930,1577,1598]" pageId="14" pageNumber="27">d</emphasis>
) and Lake de la Cruz (
<emphasis id="15F3B2637707FFE9FBBBAF7A93984D71" bold="true" box="[1150,1161,1577,1598]" pageId="14" pageNumber="27">e</emphasis>
, 
<emphasis id="15F3B2637707FFE9FB50AF7B938C4D71" bold="true" box="[1173,1181,1576,1598]" pageId="14" pageNumber="27">f</emphasis>
). Triple DAPI/FITC/CY3 set (
<emphasis id="15F3B2637707FFE9FF49AF1697894D15" bold="true" box="[140,152,1605,1626]" pageId="14" pageNumber="27">a</emphasis>
, 
<emphasis id="15F3B2637707FFE9FF61AF1697A04D15" bold="true" box="[164,177,1605,1626]" pageId="14" pageNumber="27">b</emphasis>
); DAPI set (
<emphasis id="15F3B2637707FFE9FEE8AF1696294D15" bold="true" box="[301,312,1605,1626]" pageId="14" pageNumber="27">c</emphasis>
, 
<emphasis id="15F3B2637707FFE9FE81AF16965E4D15" bold="true" box="[324,335,1605,1626]" pageId="14" pageNumber="27">e</emphasis>
), Chlorophyll 
<emphasis id="15F3B2637707FFE9FE19AF1696F94D15" box="[476,488,1605,1626]" italics="true" pageId="14" pageNumber="27">a</emphasis>
/ FITC set. Arrow: Just filled vacuole. 1 ≡ 10 µm.
</paragraph>
</caption>
<paragraph id="27386E717707FFE8FF6FAE0B95514FF3" blockId="14.[132,779,1740,1940]" lastBlockId="15.[125,772,1081,1945]" lastPageId="15" lastPageNumber="28" pageId="14" pageNumber="27">
From the point of view of picoplankton source of feed (
<figureCitation id="BFBC72F47707FFE9FF0FAE2A96324CDB" box="[202,291,1913,1940]" captionStart="Fig" captionStartId="19.[125,160,1519,1541]" captionTargetBox="[149,1437,225,1482]" captionTargetId="figure-8@19.[125,1456,206,1502]" captionTargetPageId="19" captionText="Fig. 14. a) Spirostomum teres numbers plot [cells mL–1] against autotrophic, APP vs. heterotrophic picoplankton, HPP [cells mL–1], and all analysed APP/HPP data plot, b) Species occurrence in habitats, in which S. teres was found (in the logarithmic scale of APP)." figureDoi="http://doi.org/10.5281/zenodo.10994503" httpUri="https://zenodo.org/record/10994503/files/figure.png" pageId="14" pageNumber="27">Fig. 14</figureCitation>
), 
<taxonomicName id="E08715F27707FFE9FEF9AE2996854CDC" box="[316,404,1914,1940]" class="Heterotrichea" family="Spirostomidae" genus="Spirostomum" kingdom="Chromista" order="Heterotrichida" pageId="14" pageNumber="27" phylum="Ciliophora" rank="species" species="teres">
<emphasis id="15F3B2637707FFE9FEF9AE2996854CDC" box="[316,404,1914,1940]" italics="true" pageId="14" pageNumber="27">S. teres</emphasis>
</taxonomicName>
was observed upon 
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numbers varying roughly two orders of magnitude (2 × 
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to 2 × 
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cells mL 
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) with occurrence peaking within 6 × 
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to 1 × 
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cells mL 
<superScript id="D0F2C3397707FFE9FBB1AE4293944C50" attach="left" box="[1140,1157,1809,1823]" fontSize="6" pageId="14" pageNumber="27">–1</superScript>
(logarithmic scale of categories was applied). In case of 
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, the registered optimum was observed round 1.4 × 
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cells mL 
<superScript id="D0F2C3397707FFE9FA98AE05927F4C2B" attach="left" box="[1373,1390,1878,1892]" fontSize="6" pageId="14" pageNumber="27">–1</superScript>
, typically covering the interval 7.8 × 
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to 2.8 × 
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cells mL 
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(occurrence graph is not shown but dots in the 
<figureCitation id="BFBC72F47706FFE8FFB8AD0F97D04F39" box="[125,193,1116,1142]" captionStart="Fig" captionStartId="7.[125,160,1829,1851]" captionTargetBox="[153,1442,236,1744]" captionTargetId="figure-8@7.[125,1455,217,1759]" captionTargetPageId="7" captionText="Fig. 4. Representative early- (a to h) and late-stratification (i to p) of Spirostomum teres in Lake Alchichica. a, e, i, m) Temperature, T [°C], Dissolved oxygen, DO [mg L–1], and Photosynthetically active radiation PAR [%]; b, f, j, n) numbers of heterotrophic-, HPP [cells mL–1] and autotrophic picoplankton, APP [cells mL–1], and Spirostomum teres [cells L–1]; c, g, k, o) nitrites, NO –, nitrates NO – and ammonium, 2 3 NH [µmol L–1]; d, h, l, p) chlorophyll a, Chl a [µg L–1], phycoerythrin, PE [µg L–1], and phycocyanin, PC [ng L–1]." figureDoi="http://doi.org/10.5281/zenodo.10994483" httpUri="https://zenodo.org/record/10994483/files/figure.png" pageId="15" pageNumber="28">Fig. 4</figureCitation>
represent 
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vs. all 
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data). The lowest 
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numbers were observed in the anaerobic hypolimnion while the highest ones in the oxycline.
</paragraph>
<caption id="73F83EF97706FFE8FFB8AA2F966E48E1" ID-DOI="http://doi.org/10.5281/zenodo.10994497" ID-Zenodo-Dep="10994497" httpUri="https://zenodo.org/record/10994497/files/figure.png" pageId="15" pageNumber="28" startId="15.[125,160,892,914]" targetBox="[133,756,232,852]" targetPageId="15" targetType="figure">
<paragraph id="27386E717706FFE8FFB8AA2F966E48E1" blockId="15.[125,771,892,942]" pageId="15" pageNumber="28">
<emphasis id="15F3B2637706FFE8FFB8AA2F969A48DD" bold="true" box="[125,395,892,914]" pageId="15" pageNumber="28">
Fig. 11. 
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<emphasis id="15F3B2637706FFE8FF15AA2F969A48DD" bold="true" box="[208,395,892,914]" italics="true" pageId="15" pageNumber="28">Spirostomum teres</emphasis>
</taxonomicName>
</emphasis>
occurrence (%) in habitats defined by DO concentration (mgL 
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).
</paragraph>
</caption>
<paragraph id="27386E717706FFE8FF67AD9694154D73" blockId="15.[125,772,1081,1945]" pageId="15" pageNumber="28">
In the plot of 
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<emphasis id="15F3B2637706FFE8FE85AD9696844F90" box="[320,405,1221,1247]" italics="true" pageId="15" pageNumber="28">S. teres</emphasis>
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numbers against 
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and 
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(
<figureCitation id="BFBC72F47706FFE8FF40ADBB97CC4E4C" box="[133,221,1256,1283]" captionStart="Fig" captionStartId="20.[132,167,1484,1506]" captionTargetBox="[162,1450,225,1443]" captionTargetId="figure-7@20.[134,1465,202,1463]" captionTargetPageId="20" captionText="Fig. 15. a) Spirostomum teres numbers plot [cells mL–1] against Photosynthetically Active Radiation, PAR [%] vs. autotrophic picoplankton, APP [cells mL–1], and analysed PAR/APP data plot; blue bubbles mark the samples from the event of incomplete mixing (January); b) Species occurrence in habitats, in which S. teres was found (in the logarithmic scale of PAR)." figureDoi="http://doi.org/10.5281/zenodo.10994505" httpUri="https://zenodo.org/record/10994505/files/figure.png" pageId="15" pageNumber="28">Fig. 15</figureCitation>
) we could confirm that the ciliate occurrence upon above mentioned 
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numbers is also supported with the available radiation in the layer / habitat, excluding the samples from uncomplete mixing event. According to occurrence, 
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<emphasis id="15F3B2637706FFE8FE79AC2795064EC2" box="[444,535,1396,1422]" italics="true" pageId="15" pageNumber="28">S. teres</emphasis>
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preferred 
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between 0.04 and 0.6%, with slight preference for 0.2 to 0.6% interval. However, the occurrence of 
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<emphasis id="15F3B2637706FFE8FDBDACE995DF4E9C" box="[632,718,1466,1492]" italics="true" pageId="15" pageNumber="28">S. teres</emphasis>
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was also considerable in the dark hypolimnion and bottom. While the ciliate was more common in the hypolimnion, higher numbers were found in above the bottom-layer.
</paragraph>
<paragraph id="27386E717706FFE8FF67AF1694804AA2" blockId="15.[125,772,1081,1945]" lastBlockId="15.[809,1456,223,493]" pageId="15" pageNumber="28">
The dbRDA analysis of the Lake Alchichica samples where 
<taxonomicName id="E08715F27706FFE8FEC7AF3B96464DCD" box="[258,343,1640,1666]" class="Heterotrichea" family="Spirostomidae" genus="Spirostomum" kingdom="Chromista" order="Heterotrichida" pageId="15" pageNumber="28" phylum="Ciliophora" rank="species" species="teres">
<emphasis id="15F3B2637706FFE8FEC7AF3B96464DCD" box="[258,343,1640,1666]" italics="true" pageId="15" pageNumber="28">S. teres</emphasis>
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was present covered the layers from 21 to 61 meters (the very bottom). The results (pooled in the 
<figureCitation id="BFBC72F47706FFE8FF02AFFD960A4D86" box="[199,283,1710,1737]" captionStart="Fig" captionStartId="21.[125,160,1313,1335]" captionTargetBox="[153,1440,252,1282]" captionTargetId="figure-8@21.[125,1456,223,1296]" captionTargetPageId="21" captionText="Fig. 16. a) Plot of distance-based redundancy analysis (dbRDA) of Spirostomum teres (S. teres), autotrophic picoplankton (APP) and heterotrophic picoplankton (HPP) abundance using environmental data as predictor variables, for Lake Alchichica. Environmental variables: Concentrations of dissolved oxygen (DO), ammonium (NH 3), nitrite (NO 2), nitrate (NO 3), dissolved reactive phosphorus (DRP) and silicon (SiO )." figureDoi="http://doi.org/10.5281/zenodo.10994507" httpUri="https://zenodo.org/record/10994507/files/figure.png" pageId="15" pageNumber="28">Fig. 16</figureCitation>
) show that the first axis of the ordination (dbRDA1), explained the higher percentage of the variation (88.6%), and was polarized by the concentrations of dissolved reactive phosphorus (DRP) and nitrate (
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). The second axis (dbRDA2) explained a much smaller proportion of the variation (7.8%) and can be associated to the oxic-anoxic gradient. The abundance of 
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and 
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<emphasis id="15F3B2637706FFE8FC03A9B393314BB6" box="[966,1056,224,250]" italics="true" pageId="15" pageNumber="28">S. teres</emphasis>
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is associated to microaerophilic and anoxic phosphorus-rich environments with higher nitrite (
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) and ammonium (
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) concentrations. However, in the ordination plot 
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is located in the opposite side of DRP, in environments with the lowest concentrations of phosphorus and with a higher concentration of oxygen and nitrate within the oxic-anoxic gradient.
</paragraph>
</subSubSection>
</treatment>
</document>