269 lines
38 KiB
XML
269 lines
38 KiB
XML
<document id="24B108D064F62372E2154F7A62D5B31B" ID-DOI="10.11646/zootaxa.3887.2.3" ID-GBIF-Dataset="190e4917-516a-4032-809a-42471bf7a52c" ID-ISSN="1175-5326" ID-Zenodo-Dep="229268" ID-ZooBank="B697D6BA-836B-44E2-A8D9-07661554FE59" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.tables_requiresApprovalFor="existingObjects,plazi" IM.taxonomicNames_approvedBy="felipe" checkinTime="1460976521246" checkinUser="plazi" docAuthor="Laamiri, Sayef" docDate="2014" docId="038887C4FFC1FFDDB3BBFF1583E842C1" docLanguage="en" docName="zt03887p190.pdf" docOrigin="Zootaxa 3887 (2)" docStyle="DocumentStyle:647186512141C8FC8976D5BCC54AEB7D.9:Zootaxa.2013-.journal_article" docStyleId="647186512141C8FC8976D5BCC54AEB7D" docStyleName="Zootaxa.2013-.journal_article" docStyleVersion="9" docTitle="Ceratomyxa pallida Thelohan 1895" docType="treatment" docVersion="8" lastPageNumber="162" masterDocId="FFB1FFBCFFC4FFDBB32CFF8281304349" masterDocTitle="New observations on Myxozoa of the goldline sea bream Sarpa salpa L. 1758 (Teleostei: Sparidae) from the Mediterranean coast of Tunisia" masterLastPageNumber="190" masterPageNumber="157" pageNumber="162" updateTime="1698559735493" updateUser="plazi">
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<mods:title id="E633ABFA5F0B6263965746CDEB207F77">New observations on Myxozoa of the goldline sea bream Sarpa salpa L. 1758 (Teleostei: Sparidae) from the Mediterranean coast of Tunisia</mods:title>
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<mods:namePart id="1BD0F80B5C77D9249F60C22FD3F78319">Laamiri, Sayef</mods:namePart>
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<mods:title id="9DEEE310250B2959EE51C68C60C18385">Zootaxa</mods:title>
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<mods:part id="5FAD858F07C40D99AB12F42FAF8C121B">
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<mods:date id="069AA417F252C64F237BC0DA91008150">2014</mods:date>
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<mods:number id="EB2A3CBCE86AC1D6616BA904F6565D9D">3887</mods:number>
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<mods:identifier id="9AC8B0F1305E6948355F6172901E50CB" type="DOI">10.11646/zootaxa.3887.2.3</mods:identifier>
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<treatment id="038887C4FFC1FFDDB3BBFF1583E842C1" ID-DOI="http://doi.org/10.5281/zenodo.5626242" ID-GBIF-Taxon="119594201" ID-Zenodo-Dep="5626242" LSID="urn:lsid:plazi:treatment:038887C4FFC1FFDDB3BBFF1583E842C1" httpUri="http://treatment.plazi.org/id/038887C4FFC1FFDDB3BBFF1583E842C1" lastPageId="6" lastPageNumber="162" pageId="5" pageNumber="162">
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<subSubSection id="C33B6559FFC1FFDEB3BBFF15837B43FB" box="[151,587,151,178]" pageId="5" pageNumber="162" type="nomenclature">
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<paragraph id="8B9E36D2FFC1FFDEB3BBFF15837B43FB" blockId="5.[151,587,151,178]" box="[151,587,151,178]" pageId="5" pageNumber="162">
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<heading id="D0D681BEFFC1FFDEB3BBFF15837B43FB" bold="true" box="[151,587,151,178]" fontSize="11" level="1" pageId="5" pageNumber="162" reason="1">
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<taxonomicName id="4C214D51FFC1FFDEB3BBFF15837B43FB" authority="Thelohan, 1895" authorityName="Thelohan" authorityYear="1895" box="[151,587,151,178]" class="Myxosporea" family="Ceratomyxidae" genus="Ceratomyxa" kingdom="Animalia" order="Bivalvulida" pageId="5" pageNumber="162" phylum="Myxozoa" rank="species" species="pallida">
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<emphasis id="B955EAC0FFC1FFDEB3BBFF15837B43FB" bold="true" box="[151,587,151,178]" pageId="5" pageNumber="162">
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<emphasis id="B955EAC0FFC1FFDEB3BBFF1580B443F8" bold="true" box="[151,388,151,177]" italics="true" pageId="5" pageNumber="162">Ceratomyxa pallida</emphasis>
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<bibRefCitation id="EFB04B23FFC1FFDEB2A7FF1A837B43FB" author="Thelohan" box="[395,587,152,178]" pageId="5" pageNumber="162" refString="Thelohan, P. (1895) Recherches sur les Myxosporidies. Bulletin Scientifique de la France et de la Belgique, 26, 100 - 394." type="journal article" year="1895">Thélohan, 1895</bibRefCitation>
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</emphasis>
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||
</taxonomicName>
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</heading>
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</paragraph>
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||
</subSubSection>
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<subSubSection id="C33B6559FFC1FFDEB3BBFF5C81CB4666" pageId="5" pageNumber="162" type="materials_examined">
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<paragraph id="8B9E36D2FFC1FFDEB3BBFF5C857043BE" blockId="5.[151,1437,222,715]" box="[151,1088,222,247]" pageId="5" pageNumber="162">
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||
<emphasis id="B955EAC0FFC1FFDEB3BBFF5C802343BE" bold="true" box="[151,275,222,247]" pageId="5" pageNumber="162">
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<typeStatus id="549A8870FFC1FFDEB3BBFF5C81E243BE" box="[151,210,222,247]" pageId="5" pageNumber="162">Type</typeStatus>
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host:
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</emphasis>
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<taxonomicName id="4C214D51FFC1FFDEB237FF5D836543BE" authority="Linnaeus, 1758" authorityName="Linnaeus" authorityYear="1758" box="[283,597,222,247]" class="Actinopterygii" family="Sparidae" genus="Sarpa" kingdom="Animalia" order="Perciformes" pageId="5" pageNumber="162" phylum="Chordata" rank="species" species="salpa">
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<emphasis id="B955EAC0FFC1FFDEB237FF5D809043BE" box="[283,416,222,247]" italics="true" pageId="5" pageNumber="162">Sarpa salpa</emphasis>
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Linnaeus, 1758
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</taxonomicName>
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goldline sea bream (
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<taxonomicName id="4C214D51FFC1FFDEB012FF5C82FC43BE" box="[830,972,222,247]" class="Actinopterygii" kingdom="Animalia" order="Perciformes" pageId="5" pageNumber="162" phylum="Chordata" rank="order">Perciformes</taxonomicName>
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:
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<taxonomicName id="4C214D51FFC1FFDEB0FAFF5C850A43BE" box="[982,1082,222,247]" class="Actinopterygii" family="Sparidae" kingdom="Animalia" order="Perciformes" pageId="5" pageNumber="162" phylum="Chordata" rank="family">Sparidae</taxonomicName>
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)
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</paragraph>
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<paragraph id="8B9E36D2FFC1FFDEB3EBFE8083D74252" blockId="5.[151,1437,222,715]" box="[199,743,258,283]" pageId="5" pageNumber="162">
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<emphasis id="B955EAC0FFC1FFDEB3EBFE8080604252" bold="true" box="[199,336,258,283]" pageId="5" pageNumber="162">Other host:</emphasis>
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<taxonomicName id="4C214D51FFC1FFDEB27BFE8680AD4252" box="[343,413,260,283]" class="Actinopterygii" family="Sparidae" genus="Sparus" kingdom="Animalia" order="Perciformes" pageId="5" pageNumber="179" phylum="Chordata" rank="species" species="boops">
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<emphasis id="B955EAC0FFC1FFDEB27BFE8680AD4252" box="[343,413,260,283]" italics="true" pageId="5" pageNumber="162">Boops</emphasis>
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</taxonomicName>
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<taxonomicName id="4C214D51FFC1FFDEB288FE8083AB4252" authority="Linnaeus, 1758" authorityName="Linnaeus" authorityYear="1758" box="[420,667,258,283]" class="Actinopterygii" family="Sparidae" genus="Sparus" kingdom="Animalia" order="Perciformes" pageId="5" pageNumber="162" phylum="Chordata" rank="species" species="boops">
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<emphasis id="B955EAC0FFC1FFDEB288FE8080D74252" box="[420,487,258,283]" italics="true" pageId="5" pageNumber="162">boops</emphasis>
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Linnaeus, 1758
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</taxonomicName>
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bogue
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</paragraph>
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<paragraph id="8B9E36D2FFC1FFDEB3EBFEA483CF4277" blockId="5.[151,1437,222,715]" box="[199,767,294,319]" pageId="5" pageNumber="162">
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<emphasis id="B955EAC0FFC1FFDEB3EBFEA480594276" bold="true" box="[199,361,294,319]" pageId="5" pageNumber="162">
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<typeStatus id="549A8870FFC1FFDEB3EBFEA480324276" box="[199,258,294,319]" pageId="5" pageNumber="162">Type</typeStatus>
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locality:
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</emphasis>
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Mediterranean off
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<collectingCountry id="F3367642FFC1FFDEB16DFEA583AF4277" box="[577,671,295,318]" name="Monaco" pageId="5" pageNumber="162">Monaco</collectingCountry>
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,
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<collectingCountry id="F3367642FFC1FFDEB187FEA583CB4277" box="[683,763,295,318]" name="France" pageId="5" pageNumber="162">France</collectingCountry>
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.
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</paragraph>
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<paragraph id="8B9E36D2FFC1FFDEB3EBFEC880C342CE" blockId="5.[151,1437,222,715]" pageId="5" pageNumber="162">
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<emphasis id="B955EAC0FFC1FFDEB3EBFEC880B8422A" bold="true" box="[199,392,330,355]" pageId="5" pageNumber="162">Other localities:</emphasis>
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Mediterranean off
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<collectingCountry id="F3367642FFC1FFDEB148FEC8838C422A" box="[612,700,330,355]" name="Tunisia" pageId="5" pageNumber="162">Tunisia</collectingCountry>
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: Location 1: Gulf of
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<collectingCountry id="F3367642FFC1FFDEB081FEC882DC422A" box="[941,1004,330,355]" name="Tunisia" pageId="5" pageNumber="162">Tunis</collectingCountry>
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(
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<geoCoordinate id="EE155015FFC1FFDEB0D1FEC88550422A" box="[1021,1120,330,355]" direction="north" orientation="latitude" pageId="5" pageNumber="162" precision="925" value="36.75">36°45’N</geoCoordinate>
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,
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<geoCoordinate id="EE155015FFC1FFDEB742FEC885FE422A" box="[1134,1230,330,355]" direction="east" orientation="longitude" pageId="5" pageNumber="162" precision="925" value="10.25">10°15’E</geoCoordinate>
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); Location 2: Bay of Bizerte (
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<geoCoordinate id="EE155015FFC1FFDEB23AFEED80B142CF" box="[278,385,367,391]" direction="north" orientation="latitude" pageId="5" pageNumber="162" precision="925" value="37.333332">37°20’ N</geoCoordinate>
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,
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<geoCoordinate id="EE155015FFC1FFDEB2A0FEEC80D642CE" box="[396,486,366,391]" direction="east" orientation="longitude" pageId="5" pageNumber="162" precision="925" value="9.883333">9°53’ E</geoCoordinate>
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).
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</paragraph>
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<paragraph id="8B9E36D2FFC1FFDEB3EBFE10835A42E2" blockId="5.[151,1437,222,715]" box="[199,618,402,427]" pageId="5" pageNumber="162">
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<emphasis id="B955EAC0FFC1FFDEB3EBFE1080BB42E2" bold="true" box="[199,395,402,427]" pageId="5" pageNumber="162">Site of infection:</emphasis>
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Within gall bladder
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</paragraph>
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<paragraph id="8B9E36D2FFC1FFDEB3EBFE3482D34116" blockId="5.[151,1437,222,715]" pageId="5" pageNumber="162">
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<emphasis id="B955EAC0FFC1FFDEB3EBFE3480624286" bold="true" box="[199,338,438,463]" pageId="5" pageNumber="162">Prevalence:</emphasis>
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The overall prevalence is 20% (66/330) (
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<figureCitation id="131A2A57FFC1FFDEB011FE3482B54286" box="[829,901,438,463]" captionStart="FIGURE 9" captionStartId="25.[151,250,783,805]" captionTargetBox="[325,1259,201,752]" captionTargetId="figure@25.[319,1267,195,759]" captionTargetPageId="25" captionText="FIGURE 9. Prevalence of species of myxozoans from 2 localities, Gulf of Tunis and Bay of Bizerte. The total prevalence determined within the pooled sample of 330 goldline sea bream (Sarpa salpa)." httpUri="https://zenodo.org/record/229279/files/figure.png" pageId="5" pageNumber="162">Fig. 9</figureCitation>
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). At location 1, the prevalence of infection is 22.86% (48/210) distributed as following, 03/2012: 13.3% (4/30); 04/2012: 20% (6/30); 05/2012: 30% (9/30); 06/ 2012: 23.3% (7/30); 07/2012: 20% (6/30); 08/2012: 10% (3/30); 05/2013: 40% (8/20); 06/2013: 50% (5/10). At location 2, the prevalence of infection is 15% (18/120) distributed as following, 03/2013: 13.3% (4/30); 04/2013: 13.3% (4/30); 05/2013: 16.7% (5/30); 06/2013: 16.7% (5/30) (see Table 4).
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</paragraph>
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<paragraph id="8B9E36D2FFC1FFDEB3EBFDE8854741CA" blockId="5.[151,1437,222,715]" box="[199,1143,618,643]" pageId="5" pageNumber="162">
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<emphasis id="B955EAC0FFC1FFDEB3EBFDE880B241CA" bold="true" box="[199,386,618,643]" pageId="5" pageNumber="162">Mean intensity:</emphasis>
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126.9 ± 20.8 spores/infected fish (++++++) (
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<figureCitation id="131A2A57FFC1FFDEB0ACFDE882E141CA" box="[896,977,618,643]" captionStart="FIGURE 10" captionStartId="25.[151,250,1431,1453]" captionTargetBox="[327,1259,891,1406]" captionTargetId="figure@25.[321,1266,888,1407]" captionTargetPageId="25" captionText="FIGURE 10. Mean intensity ± standard deviation of species of myxozoans infecting Sarpa Salpa." httpUri="https://zenodo.org/record/229280/files/figure.png" pageId="5" pageNumber="162">Fig. 10</figureCitation>
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) (see Table 4).
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</paragraph>
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<paragraph id="8B9E36D2FFC1FFDEB3EBFD0C82E34182" blockId="5.[151,1437,222,715]" pageId="5" pageNumber="162">
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<emphasis id="B955EAC0FFC1FFDEB3EBFD0C804941EE" bold="true" box="[199,377,654,679]" pageId="5" pageNumber="162">Type-material:</emphasis>
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Digitized photos of
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<typeStatus id="549A8870FFC1FFDEB15DFD0D83F841EE" box="[625,712,655,679]" pageId="5" pageNumber="162" type="syntype">syntype</typeStatus>
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spores were deposited in the parasitological collection of the Museum National d’Histoire Naturelle (
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<collectionCode id="ED30AE17FFC1FFDEB17BFD3183824182" LSID="urn:lsid:biocol.org:col:34988" box="[599,690,691,715]" httpUri="http://biocol.org/urn:lsid:biocol.org:col:34988" name="Museum National d'Histoire Naturelle" pageId="5" pageNumber="162">MNHN</collectionCode>
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), Paris, Coll. No. ZS 129.
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</paragraph>
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<paragraph id="8B9E36D2FFC1FFDEB3BBFD78802A405A" blockId="5.[151,1437,762,1255]" box="[151,282,762,787]" pageId="5" pageNumber="162">Description</paragraph>
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<paragraph id="8B9E36D2FFC1FFDEB3EBFC9C855E408E" blockId="5.[151,1437,762,1255]" pageId="5" pageNumber="162">
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<emphasis id="B955EAC0FFC1FFDEB3EBFC9C80A9407E" bold="true" box="[199,409,798,823]" pageId="5" pageNumber="162">Vegetative stages.</emphasis>
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Numerous spherical trophozoïtes found often in massive groups with variety of size (
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<figureCitation id="131A2A57FFC1FFDEB643FC9C81964012" captionStart="FIGURE 2" captionStartId="6.[151,250,1743,1765]" captionTargetBox="[346,1244,450,1715]" captionTargetId="figure@6.[151,1436,430,1737]" captionTargetPageId="6" captionText="FIGURE 2. Photomicrographs of Ceratomyxa pallida from the gall bladder of Sarpa salpa. (A) Spherical trophozoïtes (T) in different size attached to each other in massive groups. (B) Spherical trophozoïte with numerous inner generative cells (gc). (C – D) monosporic plasmodia (p) with fine granular protoplasm, notice the presence of filopodia (f) at the amoeboid plasmodium. (E) Fresh mature spores in sutural view presenting the polar capsules (pc) and sporoplasm nuclei (sn). (F) Fresh mature spore in lateral view. (G) Fresh mature spore in apical view. (H) Mature spore stained with Giemsa showing the distinct spherical polar capsule with visible suture line. Scale bar = 20 µm in A, C – D; 10 µm in B, E – H." httpUri="https://zenodo.org/record/229270/files/figure.png" pageId="5" pageNumber="162">Fig. 2</figureCitation>
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A). They were seen floating in the bile and contained within several refractile granules and inner generative cells (
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<figureCitation id="131A2A57FFC1FFDEB3B3FCE481D64036" box="[159,230,870,895]" captionStart="FIGURE 2" captionStartId="6.[151,250,1743,1765]" captionTargetBox="[346,1244,450,1715]" captionTargetId="figure@6.[151,1436,430,1737]" captionTargetPageId="6" captionText="FIGURE 2. Photomicrographs of Ceratomyxa pallida from the gall bladder of Sarpa salpa. (A) Spherical trophozoïtes (T) in different size attached to each other in massive groups. (B) Spherical trophozoïte with numerous inner generative cells (gc). (C – D) monosporic plasmodia (p) with fine granular protoplasm, notice the presence of filopodia (f) at the amoeboid plasmodium. (E) Fresh mature spores in sutural view presenting the polar capsules (pc) and sporoplasm nuclei (sn). (F) Fresh mature spore in lateral view. (G) Fresh mature spore in apical view. (H) Mature spore stained with Giemsa showing the distinct spherical polar capsule with visible suture line. Scale bar = 20 µm in A, C – D; 10 µm in B, E – H." httpUri="https://zenodo.org/record/229270/files/figure.png" pageId="5" pageNumber="162">Fig. 2</figureCitation>
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A), Monosporic plasmodia (n = 30) (
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<figureCitation id="131A2A57FFC1FFDEB1B6FCE483DB4036" box="[666,747,870,895]" captionStart="FIGURE 2" captionStartId="6.[151,250,1743,1765]" captionTargetBox="[346,1244,450,1715]" captionTargetId="figure@6.[151,1436,430,1737]" captionTargetPageId="6" captionText="FIGURE 2. Photomicrographs of Ceratomyxa pallida from the gall bladder of Sarpa salpa. (A) Spherical trophozoïtes (T) in different size attached to each other in massive groups. (B) Spherical trophozoïte with numerous inner generative cells (gc). (C – D) monosporic plasmodia (p) with fine granular protoplasm, notice the presence of filopodia (f) at the amoeboid plasmodium. (E) Fresh mature spores in sutural view presenting the polar capsules (pc) and sporoplasm nuclei (sn). (F) Fresh mature spore in lateral view. (G) Fresh mature spore in apical view. (H) Mature spore stained with Giemsa showing the distinct spherical polar capsule with visible suture line. Scale bar = 20 µm in A, C – D; 10 µm in B, E – H." httpUri="https://zenodo.org/record/229270/files/figure.png" pageId="5" pageNumber="162">Figs. 2</figureCitation>
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C–D), mostly spherical to amoeboid, measuring 22.9 ± 2.1 (20–25) µm in diameter. The amoeboid plasmodia showed a pseudopodia extending from its periphery (
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<figureCitation id="131A2A57FFC1FFDEB610FC0884B040EA" box="[1340,1408,906,931]" captionStart="FIGURE 2" captionStartId="6.[151,250,1743,1765]" captionTargetBox="[346,1244,450,1715]" captionTargetId="figure@6.[151,1436,430,1737]" captionTargetPageId="6" captionText="FIGURE 2. Photomicrographs of Ceratomyxa pallida from the gall bladder of Sarpa salpa. (A) Spherical trophozoïtes (T) in different size attached to each other in massive groups. (B) Spherical trophozoïte with numerous inner generative cells (gc). (C – D) monosporic plasmodia (p) with fine granular protoplasm, notice the presence of filopodia (f) at the amoeboid plasmodium. (E) Fresh mature spores in sutural view presenting the polar capsules (pc) and sporoplasm nuclei (sn). (F) Fresh mature spore in lateral view. (G) Fresh mature spore in apical view. (H) Mature spore stained with Giemsa showing the distinct spherical polar capsule with visible suture line. Scale bar = 20 µm in A, C – D; 10 µm in B, E – H." httpUri="https://zenodo.org/record/229270/files/figure.png" pageId="5" pageNumber="162">Fig. 2</figureCitation>
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C) while other spherical seemed disadvantaged from any
|
||
<typeStatus id="549A8870FFC1FFDEB1D4FC2D8218408E" box="[760,808,943,967]" pageId="5" pageNumber="162">type</typeStatus>
|
||
of pseudopodia (
|
||
<figureCitation id="131A2A57FFC1FFDEB0C6FC2C8508408E" box="[1002,1080,942,967]" captionStart="FIGURE 2" captionStartId="6.[151,250,1743,1765]" captionTargetBox="[346,1244,450,1715]" captionTargetId="figure@6.[151,1436,430,1737]" captionTargetPageId="6" captionText="FIGURE 2. Photomicrographs of Ceratomyxa pallida from the gall bladder of Sarpa salpa. (A) Spherical trophozoïtes (T) in different size attached to each other in massive groups. (B) Spherical trophozoïte with numerous inner generative cells (gc). (C – D) monosporic plasmodia (p) with fine granular protoplasm, notice the presence of filopodia (f) at the amoeboid plasmodium. (E) Fresh mature spores in sutural view presenting the polar capsules (pc) and sporoplasm nuclei (sn). (F) Fresh mature spore in lateral view. (G) Fresh mature spore in apical view. (H) Mature spore stained with Giemsa showing the distinct spherical polar capsule with visible suture line. Scale bar = 20 µm in A, C – D; 10 µm in B, E – H." httpUri="https://zenodo.org/record/229270/files/figure.png" pageId="5" pageNumber="162">Figs. 2</figureCitation>
|
||
C,D)
|
||
</paragraph>
|
||
<paragraph id="8B9E36D2FFC1FFDEB3EBFC50829047AE" blockId="5.[151,1437,762,1255]" pageId="5" pageNumber="162">
|
||
<emphasis id="B955EAC0FFC1FFDEB3EBFC50802740A2" bold="true" box="[199,279,978,1003]" pageId="5" pageNumber="162">Spores</emphasis>
|
||
(n = 30 fresh spores). Mature spores were slightly crescent-shaped with anterior margin convex and posterior slightly concave in sutural view (
|
||
<figureCitation id="131A2A57FFC1FFDEB159FC7483F24746" box="[629,706,1014,1039]" captionStart="FIGURE 2" captionStartId="6.[151,250,1743,1765]" captionTargetBox="[346,1244,450,1715]" captionTargetId="figure@6.[151,1436,430,1737]" captionTargetPageId="6" captionText="FIGURE 2. Photomicrographs of Ceratomyxa pallida from the gall bladder of Sarpa salpa. (A) Spherical trophozoïtes (T) in different size attached to each other in massive groups. (B) Spherical trophozoïte with numerous inner generative cells (gc). (C – D) monosporic plasmodia (p) with fine granular protoplasm, notice the presence of filopodia (f) at the amoeboid plasmodium. (E) Fresh mature spores in sutural view presenting the polar capsules (pc) and sporoplasm nuclei (sn). (F) Fresh mature spore in lateral view. (G) Fresh mature spore in apical view. (H) Mature spore stained with Giemsa showing the distinct spherical polar capsule with visible suture line. Scale bar = 20 µm in A, C – D; 10 µm in B, E – H." httpUri="https://zenodo.org/record/229270/files/figure.png" pageId="5" pageNumber="162">Figs. 2</figureCitation>
|
||
E–F,H,8B), measuring 7.32 ± 0.61 (6.5–8) µm in length and 28 ± 1.5 (26–30) µm in thickness. Posterior angle was concave to straight 160.9 ± 4.6 (154–170°). Two valves, roughly equal, smoothly ovoid in lateral view tapering gradually toward the end, which one rarely more attenuated than the other. Straight sutural line visible between valves. A binucleate sporoplasm with several visible sporolasmosomes occupied almost the entire spore cavity and rarely dispersed asymmetrically (
|
||
<figureCitation id="131A2A57FFC1FFDEB0D2FB04857247D6" box="[1022,1090,1158,1183]" captionStart="FIGURE 2" captionStartId="6.[151,250,1743,1765]" captionTargetBox="[346,1244,450,1715]" captionTargetId="figure@6.[151,1436,430,1737]" captionTargetPageId="6" captionText="FIGURE 2. Photomicrographs of Ceratomyxa pallida from the gall bladder of Sarpa salpa. (A) Spherical trophozoïtes (T) in different size attached to each other in massive groups. (B) Spherical trophozoïte with numerous inner generative cells (gc). (C – D) monosporic plasmodia (p) with fine granular protoplasm, notice the presence of filopodia (f) at the amoeboid plasmodium. (E) Fresh mature spores in sutural view presenting the polar capsules (pc) and sporoplasm nuclei (sn). (F) Fresh mature spore in lateral view. (G) Fresh mature spore in apical view. (H) Mature spore stained with Giemsa showing the distinct spherical polar capsule with visible suture line. Scale bar = 20 µm in A, C – D; 10 µm in B, E – H." httpUri="https://zenodo.org/record/229270/files/figure.png" pageId="5" pageNumber="162">Fig. 2</figureCitation>
|
||
E). Polar capsules were almost spherical, 2.95 ± 0.47 (2.5–3.6) µm in length and 2.92 ± 0.39 (2.5–3.6) µm in width (n = 30). The polar filament formed four turns arranged along the longitudinal axis of the capsule.
|
||
</paragraph>
|
||
<paragraph id="8B9E36D2FFC1FFDEB3BBFA9481CB4666" blockId="5.[151,1436,1302,1903]" box="[151,251,1302,1327]" pageId="5" pageNumber="162">Remarks</paragraph>
|
||
</subSubSection>
|
||
<subSubSection id="C33B6559FFC1FFDDB3EBFAB983E842C1" lastPageId="6" lastPageNumber="163" pageId="5" pageNumber="162" type="reference_group">
|
||
<paragraph id="8B9E36D2FFC1FFDEB3EBFAB9850E4426" blockId="5.[151,1436,1302,1903]" pageId="5" pageNumber="162">
|
||
<taxonomicName id="4C214D51FFC1FFDEB3EBFAB980C4461A" authority="Thelohan, 1895" authorityName="Thelohan" authorityYear="1895" box="[199,500,1338,1363]" class="Myxosporea" family="Ceratomyxidae" genus="Ceratomyxa" kingdom="Animalia" order="Bivalvulida" pageId="5" pageNumber="162" phylum="Myxozoa" rank="species" species="pallida">
|
||
<emphasis id="B955EAC0FFC1FFDEB3EBFAB9800A461A" box="[199,314,1338,1363]" italics="true" pageId="5" pageNumber="162">C. pallida</emphasis>
|
||
<bibRefCitation id="EFB04B23FFC1FFDEB26EFAB880C4461A" author="Thelohan" box="[322,500,1338,1363]" pageId="5" pageNumber="162" refString="Thelohan, P. (1895) Recherches sur les Myxosporidies. Bulletin Scientifique de la France et de la Belgique, 26, 100 - 394." type="journal article" year="1895">Thélohan, 1895</bibRefCitation>
|
||
</taxonomicName>
|
||
was firstly described from two members of
|
||
<taxonomicName id="4C214D51FFC1FFDEB0DAFAB88568461A" ID-CoL="626ZQ" box="[1014,1112,1338,1363]" class="Actinopterygii" family="Sparidae" kingdom="Animalia" order="Perciformes" pageId="5" pageNumber="162" phylum="Chordata" rank="family">Sparidae</taxonomicName>
|
||
family
|
||
<taxonomicName id="4C214D51FFC1FFDEB798FABE8428461A" ID-CoL="3BG4" box="[1204,1304,1338,1363]" class="Actinopterygii" family="Sparidae" genus="Sparus" kingdom="Animalia" order="Perciformes" pageId="5" pageNumber="162" phylum="Chordata" rank="species" species="boops">
|
||
<emphasis id="B955EAC0FFC1FFDEB798FABE8428461A" box="[1204,1304,1338,1363]" italics="true" pageId="5" pageNumber="162">B. boops</emphasis>
|
||
</taxonomicName>
|
||
(L.) and
|
||
<taxonomicName id="4C214D51FFC1FFDEB6ABFAB981E4463E" ID-CoL="79PJZ" class="Actinopterygii" family="Sparidae" genus="Sarpa" kingdom="Animalia" order="Perciformes" pageId="5" pageNumber="162" phylum="Chordata" rank="species" species="salpa">
|
||
<emphasis id="B955EAC0FFC1FFDEB6ABFAB981E4463E" italics="true" pageId="5" pageNumber="162">S. salpa</emphasis>
|
||
</taxonomicName>
|
||
(L.) from the Mediterranean off
|
||
<collectingCountry id="F3367642FFC1FFDEB168FADD83A4463F" box="[580,660,1375,1398]" name="France" pageId="5" pageNumber="162">France</collectingCountry>
|
||
. The original description of
|
||
<bibRefCitation id="EFB04B23FFC1FFDEB0FDFADC85BC463E" author="Thelohan" box="[977,1164,1374,1399]" pageId="5" pageNumber="162" refString="Thelohan, P. (1895) Recherches sur les Myxosporidies. Bulletin Scientifique de la France et de la Belgique, 26, 100 - 394." type="journal article" year="1895">Thélohan (1895)</bibRefCitation>
|
||
was poor and limited to some measures. No schematic draw has been published for this species by that author or by Georgévitch (1916) who found the same parasite in the same two hosts from the Mediterranean off
|
||
<collectingCountry id="F3367642FFC1FFDEB70FFA2585B246F7" box="[1059,1154,1447,1470]" name="Monaco" pageId="5" pageNumber="162">Monaco</collectingCountry>
|
||
.
|
||
<bibRefCitation id="EFB04B23FFC1FFDEB7A2FA24846046F6" author="Auerbach" box="[1166,1360,1446,1471]" pageId="5" pageNumber="162" refString="Auerbach, M. (1912) Studien uber die Myxosporidien der Norwegischen Seefische und ihre Verbreitung. Zoologischer Jahrbucher, Abteilung I. Systematik (Okologie), Geographie und Biologie, 34, 1 - 50." type="journal article" year="1912">Auerbach (1912)</bibRefCitation>
|
||
, in his study, was uncertain as the
|
||
<taxonomicName id="4C214D51FFC1FFDEB2F4FA49835146AB" box="[472,609,1483,1506]" class="Myxosporea" family="Ceratomyxidae" genus="Ceratomyxa" kingdom="Animalia" order="Bivalvulida" pageId="5" pageNumber="162" phylum="Myxozoa" rank="genus">
|
||
<emphasis id="B955EAC0FFC1FFDEB2F4FA49835146AB" box="[472,609,1483,1506]" italics="true" pageId="5" pageNumber="162">Ceratomyxa</emphasis>
|
||
</taxonomicName>
|
||
species found in the gallbladder of
|
||
<taxonomicName id="4C214D51FFC1FFDEB72AFA4884AC46AA" ID-CoL="6K6QB" authority="Linnaeus, 1758" authorityName="Linnaeus" authorityYear="1758" box="[1030,1436,1482,1507]" class="Actinopterygii" family="Gadidae" genus="Gadus" kingdom="Animalia" order="Gadiformes" pageId="5" pageNumber="162" phylum="Chordata" rank="species" species="merlangus">
|
||
<emphasis id="B955EAC0FFC1FFDEB72AFA4885FF46AA" box="[1030,1231,1482,1507]" italics="true" pageId="5" pageNumber="162">Gadus merlangus</emphasis>
|
||
(Linnaeus, 1758)
|
||
</taxonomicName>
|
||
from the Bergen in
|
||
<collectingCountry id="F3367642FFC1FFDEB256FA6D80E5454E" box="[378,469,1519,1543]" name="Norway" pageId="5" pageNumber="162">Norway</collectingCountry>
|
||
, was
|
||
<taxonomicName id="4C214D51FFC1FFDEB13BFA6D83BA454E" box="[535,650,1518,1543]" class="Myxosporea" family="Ceratomyxidae" genus="Ceratomyxa" kingdom="Animalia" order="Bivalvulida" pageId="5" pageNumber="162" phylum="Myxozoa" rank="species" species="pallida">
|
||
<emphasis id="B955EAC0FFC1FFDEB13BFA6D83BA454E" box="[535,650,1518,1543]" italics="true" pageId="5" pageNumber="162">C. pallida</emphasis>
|
||
</taxonomicName>
|
||
based on dimensions of vegetative forms which were disporous and some mature spores. Likewise,
|
||
<bibRefCitation id="EFB04B23FFC1FFDEB2D5F99083BB4562" author="Davis" box="[505,651,1554,1579]" pageId="5" pageNumber="162" refString="Davis, H. S. (1917) The Myxosporidia of the Beaufort region, a systematic and biological study. Bulletin of the United States Bureau of Fisheries, 35, 199 - 252." type="journal article" year="1917">Davis (1917)</bibRefCitation>
|
||
mentioned in his study a very close similarity between
|
||
<taxonomicName id="4C214D51FFC1FFDEB7D1F991802F4506" authority="Davis, 1917" authorityName="Davis" authorityYear="1917" class="Myxosporea" family="Ceratomyxidae" genus="Ceratomyxa" kingdom="Animalia" order="Bivalvulida" pageId="5" pageNumber="162" phylum="Myxozoa" rank="species" species="monospora">
|
||
<emphasis id="B955EAC0FFC1FFDEB7D1F99184AC4562" box="[1277,1436,1555,1579]" italics="true" pageId="5" pageNumber="162">C. monospora</emphasis>
|
||
<bibRefCitation id="EFB04B23FFC1FFDEB3BBF9B4802F4506" author="Davis" box="[151,287,1590,1615]" pageId="5" pageNumber="162" refString="Davis, H. S. (1917) The Myxosporidia of the Beaufort region, a systematic and biological study. Bulletin of the United States Bureau of Fisheries, 35, 199 - 252." type="journal article" year="1917">Davis, 1917</bibRefCitation>
|
||
</taxonomicName>
|
||
found in the gall bladders of
|
||
<taxonomicName id="4C214D51FFC1FFDEB15FF9B485274506" ID-CoL="797WF" authority="Linnaeus, 1766" authorityName="Linnaeus" authorityYear="1766" box="[627,1047,1590,1615]" class="Actinopterygii" family="Stromateidae" genus="Peprilus" kingdom="Animalia" order="Perciformes" pageId="5" pageNumber="162" phylum="Chordata" rank="species" species="alepidotus">
|
||
<emphasis id="B955EAC0FFC1FFDEB15FF9B4827F4506" box="[627,847,1590,1615]" italics="true" pageId="5" pageNumber="162">Peprilus alepidotus</emphasis>
|
||
(Linnaeus, 1766)
|
||
</taxonomicName>
|
||
and
|
||
<taxonomicName id="4C214D51FFC1FFDEB77CF9B581E8453A" ID-CoL="4MGG4" authority="Linnaeus, 1766" authorityName="Linnaeus" authorityYear="1766" class="Actinopterygii" family="Triglidae" genus="Prionotus" kingdom="Animalia" order="Scorpaeniformes" pageId="5" pageNumber="162" phylum="Chordata" rank="species" species="evolans">
|
||
<emphasis id="B955EAC0FFC1FFDEB77CF9B5842C4506" box="[1104,1308,1590,1615]" italics="true" pageId="5" pageNumber="162">Prionotus evolans</emphasis>
|
||
(Linnaeus, 1766)
|
||
</taxonomicName>
|
||
from Atlantic Ocean and
|
||
<taxonomicName id="4C214D51FFC1FFDEB13CF9D98273453A" authority="Thelohan, 1895" authorityName="Thelohan" authorityYear="1895" box="[528,835,1626,1651]" class="Myxosporea" family="Ceratomyxidae" genus="Ceratomyxa" kingdom="Animalia" order="Bivalvulida" pageId="5" pageNumber="162" phylum="Myxozoa" rank="species" species="pallida">
|
||
<emphasis id="B955EAC0FFC1FFDEB13CF9D983B4453A" box="[528,644,1626,1651]" italics="true" pageId="5" pageNumber="162">C. pallida</emphasis>
|
||
<bibRefCitation id="EFB04B23FFC1FFDEB1A3F9D88273453A" author="Thelohan" box="[655,835,1626,1651]" pageId="5" pageNumber="162" refString="Thelohan, P. (1895) Recherches sur les Myxosporidies. Bulletin Scientifique de la France et de la Belgique, 26, 100 - 394." type="journal article" year="1895">Thélohan, 1895</bibRefCitation>
|
||
</taxonomicName>
|
||
based only on the size of the trophozoïtes without determination of the mature spores. In the study of
|
||
<bibRefCitation id="EFB04B23FFC1FFDEB1D7F9FC82F345DE" author="Meglitsch" box="[763,963,1662,1687]" pageId="5" pageNumber="162" refString="Meglitsch, P. A. (1960) Some coelozoic Myxosporidia from New Zealand fishes. I. General, and family Ceratomyxidae." type="book" year="1960">Meglitsch (1960)</bibRefCitation>
|
||
,
|
||
<taxonomicName id="4C214D51FFC1FFDEB0FFF9FD857845DE" box="[979,1096,1662,1687]" class="Myxosporea" family="Ceratomyxidae" genus="Ceratomyxa" kingdom="Animalia" order="Bivalvulida" pageId="5" pageNumber="162" phylum="Myxozoa" rank="species" species="pallida">
|
||
<emphasis id="B955EAC0FFC1FFDEB0FFF9FD857845DE" box="[979,1096,1662,1687]" italics="true" pageId="5" pageNumber="162">C. pallida</emphasis>
|
||
</taxonomicName>
|
||
and other species have been omitted from the list of ceratomyxids because of the lack of information. Furthermore, in the synopsis of the
|
||
<taxonomicName id="4C214D51FFC1FFDEB3BBF94580104597" box="[151,288,1735,1758]" class="Myxosporea" family="Ceratomyxidae" genus="Ceratomyxa" kingdom="Animalia" order="Bivalvulida" pageId="5" pageNumber="162" phylum="Myxozoa" rank="genus">
|
||
<emphasis id="B955EAC0FFC1FFDEB3BBF94580104597" box="[151,288,1735,1758]" italics="true" pageId="5" pageNumber="162">Ceratomyxa</emphasis>
|
||
</taxonomicName>
|
||
compiled by
|
||
<bibRefCitation id="EFB04B23FFC1FFDEB2EAF94483684596" author="Eiras" box="[454,600,1734,1759]" pageId="5" pageNumber="162" refString="Eiras, J. C. (2006) Synopsis of the species of Ceratomyxa Thelohan, 1892 (Myxozoa: Myxosporea: Ceratomyxidae). Systematic Parasitology, 65 (1), 49 - 71. http: // dx. doi. org / 10.1007 / s 11230 - 006 - 9039 - 5" type="journal article" year="2006">Eiras (2006)</bibRefCitation>
|
||
, the description of
|
||
<taxonomicName id="4C214D51FFC1FFDEB013F94582844596" box="[831,948,1734,1759]" class="Myxosporea" family="Ceratomyxidae" genus="Ceratomyxa" kingdom="Animalia" order="Bivalvulida" pageId="5" pageNumber="162" phylum="Myxozoa" rank="species" species="pallida">
|
||
<emphasis id="B955EAC0FFC1FFDEB013F94582844596" box="[831,948,1734,1759]" italics="true" pageId="5" pageNumber="162">C. pallida</emphasis>
|
||
</taxonomicName>
|
||
did not conform to the data noted in the original report as the vegetative forms were almost spherical and not the mature spores (
|
||
<bibRefCitation id="EFB04B23FFC1FFDEB7B2F968847F444A" author="Thelohan" box="[1182,1359,1770,1795]" pageId="5" pageNumber="162" refString="Thelohan, P. (1895) Recherches sur les Myxosporidies. Bulletin Scientifique de la France et de la Belgique, 26, 100 - 394." type="journal article" year="1895">Thélohan 1895</bibRefCitation>
|
||
;
|
||
<bibRefCitation id="EFB04B23FFC1FFDEB672F96881E3446E" author="Kudo" pageId="5" pageNumber="162" refString="Kudo, R. (1920) Studies on Myxosporidia. A synopsis of genera and species of myxosporidia. Illinois Biological Mongraphs, 5, 1 - 265." type="journal article" year="1920">Kudo 1920</bibRefCitation>
|
||
). During this survey, the trophozoïtes of
|
||
<taxonomicName id="4C214D51FFC1FFDEB198F88D8217446E" box="[692,807,1806,1831]" class="Myxosporea" family="Ceratomyxidae" genus="Ceratomyxa" kingdom="Animalia" order="Bivalvulida" pageId="5" pageNumber="162" phylum="Myxozoa" rank="species" species="pallida">
|
||
<emphasis id="B955EAC0FFC1FFDEB198F88D8217446E" box="[692,807,1806,1831]" italics="true" pageId="5" pageNumber="162">C. pallida</emphasis>
|
||
</taxonomicName>
|
||
are spherical and monosporous in congruent with the original description of
|
||
<bibRefCitation id="EFB04B23FFC1FFDEB2B0F8B0836D4402" author="Thelohan" box="[412,605,1842,1867]" pageId="5" pageNumber="162" refString="Thelohan, P. (1895) Recherches sur les Myxosporidies. Bulletin Scientifique de la France et de la Belgique, 26, 100 - 394." type="journal article" year="1895">Thélohan (1895)</bibRefCitation>
|
||
. However, the dimensions of the present form show a little difference as the plasmodia are larger and the spores are more longer than those originally noted.
|
||
</paragraph>
|
||
<paragraph id="8B9E36D2FFC1FFDEB3BBF81C806044FF" blockId="5.[151,1436,1950,2011]" box="[151,336,1950,1975]" pageId="5" pageNumber="162">Ecological notes</paragraph>
|
||
<paragraph id="8B9E36D2FFC1FFDDB3EBF84083E842C1" blockId="5.[151,1436,1950,2011]" lastBlockId="6.[151,1436,151,392]" lastPageId="6" lastPageNumber="163" pageId="5" pageNumber="162">
|
||
During our investigation, the overall prevalence of
|
||
<taxonomicName id="4C214D51FFC1FFDEB026F841824C4492" box="[778,892,1986,2011]" class="Myxosporea" family="Ceratomyxidae" genus="Ceratomyxa" kingdom="Animalia" order="Bivalvulida" pageId="5" pageNumber="162" phylum="Myxozoa" rank="species" species="pallida">
|
||
<emphasis id="B955EAC0FFC1FFDEB026F841824C4492" box="[778,892,1986,2011]" italics="true" pageId="5" pageNumber="162">C. pallida</emphasis>
|
||
</taxonomicName>
|
||
infection is 20%. It has a parasitic status as less frequent species (10% ≤ Prevalence ≤ 50%). Infection by
|
||
<taxonomicName id="4C214D51FFC2FFDDB060FF1A82F143F9" box="[844,961,151,176]" class="Myxosporea" family="Ceratomyxidae" genus="Ceratomyxa" kingdom="Animalia" order="Bivalvulida" pageId="6" pageNumber="163" phylum="Myxozoa" rank="species" species="pallida">
|
||
<emphasis id="B955EAC0FFC2FFDDB060FF1A82F143F9" box="[844,961,151,176]" italics="true" pageId="6" pageNumber="163">C. pallida</emphasis>
|
||
</taxonomicName>
|
||
was present during the entire period of sampling in both localities. In Gulf of
|
||
<collectingCountry id="F3367642FFC2FFDDB168FF3E83B6439C" box="[580,646,188,213]" name="Tunisia" pageId="6" pageNumber="163">Tunis</collectingCountry>
|
||
, the infection started from March and increased gradually to June with greater prevalence 20% in
|
||
<date id="FF9F1012FFC2FFDDB2E2FF62836E43B1" box="[462,606,223,248]" pageId="6" pageNumber="163" value="2012" valueMax="2050">2012 and 50</date>
|
||
% in 2013. However, it decreased after from June to August. Generally, prevalence and mean intensity values increased from March to June then diminished till August. In Bay of Bizerte, the infection was detected with no significant difference between months. The highest prevalence was noted in May 16.7% and still constable to June (see Table 4). In both localities, mean intensity of
|
||
<taxonomicName id="4C214D51FFC2FFDDB75FFECF85D2422C" box="[1139,1250,332,357]" class="Myxosporea" family="Ceratomyxidae" genus="Ceratomyxa" kingdom="Animalia" order="Bivalvulida" pageId="6" pageNumber="163" phylum="Myxozoa" rank="species" species="pallida">
|
||
<emphasis id="B955EAC0FFC2FFDDB75FFECF85D2422C" box="[1139,1250,332,357]" italics="true" pageId="6" pageNumber="163">C. pallida</emphasis>
|
||
</taxonomicName>
|
||
appeared severe with 126.9 ± 20.8 spores per infected fish (
|
||
<figureCitation id="131A2A57FFC2FFDDB154FEED83FB42C1" box="[632,715,367,392]" captionStart="FIGURE 10" captionStartId="25.[151,250,1431,1453]" captionTargetBox="[327,1259,891,1406]" captionTargetId="figure@25.[321,1266,888,1407]" captionTargetPageId="25" captionText="FIGURE 10. Mean intensity ± standard deviation of species of myxozoans infecting Sarpa Salpa." httpUri="https://zenodo.org/record/229280/files/figure.png" pageId="6" pageNumber="163">Fig. 10</figureCitation>
|
||
).
|
||
</paragraph>
|
||
</subSubSection>
|
||
</treatment>
|
||
</document> |