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<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.99.110043" ID-Pensoft-Pub="1314-4049-99-319" ID-Pensoft-UUID="5D733AF189695962ABD165E810467E16" ModsDocID="1314-4049-99-319" checkinTime="1698104609360" checkinUser="pensoft" docAuthor="Lambert, Christopher, Schweizer, Lena, Matio Kemkuignou, Blondelle, Anoumedem, Elodie Gisele M., Kouam, Simeon F. &amp; Marin-Felix, Yasmina" docDate="2023" docId="6557A14B6F0B52278FBD5F45AECD35CF" docLanguage="en" docName="MycoKeys 99: 319-362" docOrigin="MycoKeys 99" docPubDate="2023-10-23" docSource="http://dx.doi.org/10.3897/mycokeys.99.110043" docTitle="Diaporthe rauvolfiae Y. Marin, C. Lamb., Kouam &amp; L. Schweizer 2023, sp. nov." docType="treatment" docVersion="1" id="5D733AF189695962ABD165E810467E16" lastPageNumber="319" masterDocId="5D733AF189695962ABD165E810467E16" masterDocTitle="Four new endophytic species of Diaporthe (Diaporthaceae, Diaporthales) isolated from Cameroon" masterLastPageNumber="362" masterPageNumber="319" pageNumber="319" updateTime="1698104609360" updateUser="pensoft">
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<mods:title id="60D58393B6F3B0F4541A59F551AC82B6">Four new endophytic species of Diaporthe (Diaporthaceae, Diaporthales) isolated from Cameroon</mods:title>
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<mods:namePart id="815034C387F8960B521F0802CB680C79">Lambert, Christopher</mods:namePart>
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<mods:affiliation id="7D2B69E552B2D208F16427CF8783BCCE">Department of Microbial Drugs, Helmholtz Centre for Infection Research (HZI) and German Centre for Infection Research (DZIF), Partner Site Hannover / Braunschweig, Inhoffenstrasse 7, 38124 Braunschweig, Germany &amp; Institute of Microbiology, Technische Universitaet Braunschweig, Spielmannstrasse 7, 38106 Braunschweig, Germany &amp; Molecular Cell Biology Group, Helmholtz Centre for Infection Research (HZI), Inhoffenstrasse 7, 38124 Braunschweig, Germany</mods:affiliation>
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<mods:namePart id="075BC7854577FF6CD0BA6E87A3D9A934">Schweizer, Lena</mods:namePart>
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<mods:affiliation id="CE4DF2EBB91A3E93ABD8543AEE475429">Department of Microbial Drugs, Helmholtz Centre for Infection Research (HZI) and German Centre for Infection Research (DZIF), Partner Site Hannover / Braunschweig, Inhoffenstrasse 7, 38124 Braunschweig, Germany</mods:affiliation>
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<mods:namePart id="165818D4947432A4F7BA18E7A115DB46">Matio Kemkuignou, Blondelle</mods:namePart>
<mods:affiliation id="8438E8BF612E9D0DF9D01D296CD81760">Department of Microbial Drugs, Helmholtz Centre for Infection Research (HZI) and German Centre for Infection Research (DZIF), Partner Site Hannover / Braunschweig, Inhoffenstrasse 7, 38124 Braunschweig, Germany &amp; Institute of Microbiology, Technische Universitaet Braunschweig, Spielmannstrasse 7, 38106 Braunschweig, Germany</mods:affiliation>
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<mods:namePart id="E2A9949C4FEE880E19A72944B928D973">Anoumedem, Elodie Gisele M.</mods:namePart>
<mods:affiliation id="6407285CBEE415FD190EF009196FBA51">Department of Chemistry, Higher Teacher Training College, University of Yaounde I, Yaounde P. O. Box 47, Cameroon</mods:affiliation>
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<mods:namePart id="78A1B5B4B6EA28345E07D748E7F4BEF2">Kouam, Simeon F.</mods:namePart>
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<mods:affiliation id="5136E2CCB0794A8CB78A8F0E9E38AE2E">Department of Chemistry, Higher Teacher Training College, University of Yaounde I, Yaounde P. O. Box 47, Cameroon</mods:affiliation>
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<mods:namePart id="D092E044D73AAD4394A959D7E92531D9">Marin-Felix, Yasmina</mods:namePart>
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<mods:affiliation id="8BC200781F1DA8F6E981FFA65B6ED823">Department of Microbial Drugs, Helmholtz Centre for Infection Research (HZI) and German Centre for Infection Research (DZIF), Partner Site Hannover / Braunschweig, Inhoffenstrasse 7, 38124 Braunschweig, Germany &amp; Institute of Microbiology, Technische Universitaet Braunschweig, Spielmannstrasse 7, 38106 Braunschweig, Germany</mods:affiliation>
<mods:nameIdentifier id="E6A234B0EE9AB743EB18C8CECD841DF0" type="email">yasmina.marinfelix@helmholtz-hzi.de</mods:nameIdentifier>
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<treatment id="6557A14B6F0B52278FBD5F45AECD35CF" LSID="urn:lsid:plazi:treatment:6557A14B6F0B52278FBD5F45AECD35CF" httpUri="http://treatment.plazi.org/id/6557A14B6F0B52278FBD5F45AECD35CF" lastPageNumber="319" pageId="0" pageNumber="319">
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<paragraph id="DCB9360C97145F8022FA26A71E736D40" pageId="0" pageNumber="319">
<taxonomicName id="E87D2DF2021C7D56F4D7C5F99B6B94AF" LSID="6557A14B-6F0B-5227-8FBD-5F45AECD35CF" authority="Y. Marín, C. Lamb., Kouam &amp; L. Schweizer" authorityName="Y. Marin, C. Lamb., Kouam &amp; L. Schweizer" authorityYear="2023" class="Ascomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe rauvolfiae" order="Diaporthales" pageId="0" pageNumber="319" phylum="Ascomycota" rank="species" species="rauvolfiae" status="sp. nov.">
Diaporthe rauvolfiae Y.
<normalizedToken id="604CE977612DA1A92220AA4533F74CF7" originalValue="Marín">Marin</normalizedToken>
, C. Lamb., Kouam &amp; L. Schweizer
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<taxonomicNameLabel id="73AB6322662CC52E8A39C99B1AD9F41D" pageId="0" pageNumber="319">sp. nov.</taxonomicNameLabel>
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<paragraph id="565FA17DF5804B871CFA9997327010DC" pageId="0" pageNumber="319">
<figureCitation id="A6939E1D27360B3708452282D39BF5FE" captionStart="Figure 7" captionStartId="F5" captionText="Figure 7. Diaporthe rauvolfiae (ex-type strain CBS 148912) A conidioma in PNA B conidiomata in OA C conidiophores and conidia D alpha, beta and alpha conidia. Scale bars: 200 μm (A); 500 μm (B); 5 μm (C, D)." figureDoi="10.3897/mycokeys.99.110043.figure7" httpUri="https://binary.pensoft.net/fig/925261" pageId="0" pageNumber="319">Fig. 7</figureCitation>
</paragraph>
</subSubSection>
<subSubSection id="66E71A3D5A20FA0EFDDC154B8ED3A010" pageId="0" pageNumber="319" type="etymology">
<paragraph id="2FD9193B7A7F5FE762F170CC4AE4E394" pageId="0" pageNumber="319">Etymology.</paragraph>
<paragraph id="DB67D9D42E4DBD9FB201B4E3B4F7C3A7" pageId="0" pageNumber="319">
Name refers to the host genus that this fungus was isolated from,
<taxonomicName id="B60AB996E2302140EEC6595DAFB0C7E1" class="Dicotyledoneae" family="Apocynaceae" genus="Rauvolfia" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="Rauvolfia" order="Gentianales" pageId="0" pageNumber="319" phylum="Angiospermae" rank="genus">
<emphasis id="678C60339A9D9E7FB56E9BBEBAF770F3" italics="true" pageId="0" pageNumber="319">Rauvolfia</emphasis>
</taxonomicName>
.
</paragraph>
<caption id="8CD8DFE7EB8BC99A8FDD486FCABB9D41" doi="10.3897/mycokeys.99.110043.figure7" httpUri="https://binary.pensoft.net/fig/925261" pageId="0" pageNumber="319" start="Figure 7" startId="F5">
<paragraph id="3F954AEFFC8E66059DC36EAE2A9F156B" pageId="0" pageNumber="319">
<emphasis id="2EE8C0B971C4065A5EB302E3B3CAC681" bold="true" pageId="0" pageNumber="319">Figure 7.</emphasis>
<taxonomicName id="BE6B04A1B2B5AD8F446AAD2DB221E37F" authorityName="Y. Marin, C. Lamb., Kouam &amp; L. Schweizer" authorityYear="2023" class="Ascomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe rauvolfiae" order="Diaporthales" pageId="0" pageNumber="319" phylum="Ascomycota" rank="species" species="rauvolfiae">
<emphasis id="3CF61CAA141B803460AF4CC8F825F0E6" italics="true" pageId="0" pageNumber="319">Diaporthe rauvolfiae</emphasis>
</taxonomicName>
(ex-type strain CBS 148912)
<emphasis id="A4BA2EBAA865007D4194C66A66CB12D7" bold="true" pageId="0" pageNumber="319">A</emphasis>
conidioma in PNA
<emphasis id="5883C76EF0635F25D40987A736F8520B" bold="true" pageId="0" pageNumber="319">B</emphasis>
conidiomata in OA
<emphasis id="BB7EE51F850EF8D4F808409DFA66453E" bold="true" pageId="0" pageNumber="319">C</emphasis>
conidiophores and conidia
<emphasis id="3BC0DF1851590BA5566BD547B9151AD4" bold="true" pageId="0" pageNumber="319">D</emphasis>
alpha, beta and alpha conidia. Scale bars: 200
<normalizedToken id="3C1FED698E258156FCF5B02E7C15A614" originalValue="μm">μm</normalizedToken>
(
<emphasis id="2D0AFC00599D1861500DDD0ED253C641" bold="true" pageId="0" pageNumber="319">A</emphasis>
); 500
<normalizedToken id="DC555F89D94FD68748D98BE67951E94A" originalValue="μm">μm</normalizedToken>
(
<emphasis id="A205807CD8F759F76DD8D8EB45E87C77" bold="true" pageId="0" pageNumber="319">B</emphasis>
); 5
<normalizedToken id="BB15A9E0B0159C13BC46847FE98276B7" originalValue="μm">μm</normalizedToken>
(
<emphasis id="AABEBBF3E0ED06C56D06B88948B543C1" bold="true" pageId="0" pageNumber="319">C, D</emphasis>
).
</paragraph>
</caption>
</subSubSection>
<subSubSection id="C8243AE74BF856F7C7E8AA0F0712D356" pageId="0" pageNumber="319" type="description">
<paragraph id="EF58106B7102BF5E9AB75E2EA8967745" pageId="0" pageNumber="319">Description.</paragraph>
<paragraph id="2B10FAB30B098C756B3DA08EC213ED76" pageId="0" pageNumber="319">
Conidiomata pycnidial in culture on PNA, globose or irregular, dark brown to black, solitary or in groups, embedded, erumpent, 210-450(-530)
<normalizedToken id="5B929E415A9E425FE52C25A02249000E" originalValue="μm">μm</normalizedToken>
diam, white to cream conidial drops exuded from ostioles; conidiomatal wall yellowish brown to olivaceous brown or brown, composed of 1-2 layers, textura angularis. Conidiophores cylindrical to subcylindrical, tapering towards apex, base subhyaline to pale yellow or pale olivaceous, apex hyaline to subhyaline, densely aggregated, smooth-walled, (0-)1-2 septate, 9-19.5
<normalizedToken id="DE3AE24BC6584F2C0E86887ECECD6AD1" originalValue="×">x</normalizedToken>
1.5-3.5
<normalizedToken id="30B7C13A1867C654227D67EF2B63E06E" originalValue="μm">μm</normalizedToken>
. Conidiogenous cells phialidic, cylindrical to subcylindrical, tapering towards apex, hyaline, mostly terminal, 6.5-13.5
<normalizedToken id="BB2399A6A077E36BD73235CE2552E14F" originalValue="×">x</normalizedToken>
1.5-3
<normalizedToken id="6F14FF9899A7F8726B712E44AE02002C" originalValue="μm">μm</normalizedToken>
. Paraphyses not observed. Alpha conidia broadly fusiform to obovoid, hyaline, apex rounded or acute, base acutate, biguttulate to multiguttulate, aseptate, 6.5-9
<normalizedToken id="487A95916488DA15D7D8B3A4623B8340" originalValue="×">x</normalizedToken>
2-3
<normalizedToken id="A364915F8A671E18A03D13EC5F438912" originalValue="μm">μm</normalizedToken>
. Beta conidia filiform, curved, tapering towards apex, hyaline, not guttulate, aseptate, 20-36.5
<normalizedToken id="4527DBF4E76994F960603726C0F57710" originalValue="×">x</normalizedToken>
1-2
<normalizedToken id="9A8CBD95CE1DD3E6C62D9C24CD3A6826" originalValue="μm">μm</normalizedToken>
. Gamma conidia less frequent, fusiform to obovoid, straight to slightly curved, rarely sinuose, acutate ends or one acutate and other round, hyaline, multiguttulate, aseptate, (8-)9-13
<normalizedToken id="77E711FCD4975881EB16776D426A09FE" originalValue="×">x</normalizedToken>
1.5-2.5
<normalizedToken id="9B5012604032CC2EE55FBE48977DD7AA" originalValue="μm">μm</normalizedToken>
.
</paragraph>
</subSubSection>
<subSubSection id="C46D00CD355F0D90A96362CF63EB38E8" pageId="0" pageNumber="319" type="description">
<paragraph id="DFEC09ED3249CC56B5E11EA9DC16386E" pageId="0" pageNumber="319">Culture characters.</paragraph>
<paragraph id="A662D8ECEEF0E109DBAAC1E4146C6687" pageId="0" pageNumber="319">Colonies on PDA reaching 72-76 mm in 2 weeks, grayed yellow (160B-C) with white ring and transparent margins, cottony to slightly feathery, raised, lobate, margins filamentous; reverse grayed yellow (160B-C) with white ring and transparent margins. Colonies on MEA covering the surface of the Petri dish in 2 weeks, white to grayed white (156B), cottony to slightly feathery, raised, margins filamentous; reverse grayed green (197A) to gray brown (199A) with black (202A) center. Colonies on OA covering the surface of the Petri dish in 2 weeks, grayed white (156B-D), cottony to slightly feathery, raised, margins filamentous; reverse gray brown (199A).</paragraph>
</subSubSection>
<subSubSection id="6523C8E0DCE1503FEFD62D332F17881B" pageId="0" pageNumber="319" type="specimen examined">
<paragraph id="8BD0A1E5C691830C3B12AD2CA4413838" pageId="0" pageNumber="319">Specimen examined.</paragraph>
<paragraph id="1160DF85B9AB56C2E6E2BAE3AD572F16" pageId="0" pageNumber="319">
Cameroon, Tonga, West Region, from
<taxonomicName id="8589E8D65DF4A71348828FC8102961EC" class="Dicotyledoneae" family="Apocynaceae" genus="Rauvolfia" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="Rauvolfia vomitoria" order="Gentianales" pageId="0" pageNumber="319" phylum="Angiospermae" rank="species" species="vomitoria">
<emphasis id="9560CC5DFF17F5C46847BFEE3B83DB00" italics="true" pageId="0" pageNumber="319">Rauvolfia vomitoria</emphasis>
</taxonomicName>
, 19 Jun. 2019,
<emphasis id="4C2CAD5E11B7DC71CDFE8DB37139A4CF" italics="true" pageId="0" pageNumber="319">E.G.M. Anoumedem</emphasis>
(holotype CBS H-24924, culture ex-type CBS 148912 = STMA 18287).
</paragraph>
</subSubSection>
<subSubSection id="E4C09F929D5CD8C5976DD7CADC2A0DF0" pageId="0" pageNumber="319" type="notes">
<paragraph id="16184EAA34450B3DB5B96339B6B4EACA" pageId="0" pageNumber="319">Notes.</paragraph>
<paragraph id="53B0C6567509241CB30096C2E30D21D1" pageId="0" pageNumber="319">
<taxonomicName id="5AE60E5BB554F22D4D19988806C7D8C5" authorityName="Y. Marin, C. Lamb., Kouam &amp; L. Schweizer" authorityYear="2023" class="Ascomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe rauvolfiae" order="Diaporthales" pageId="0" pageNumber="319" phylum="Ascomycota" rank="species" species="rauvolfiae">
<emphasis id="C86B653A19D875249C1F4540DE77710E" italics="true" pageId="0" pageNumber="319">Diaporthe rauvolfiae</emphasis>
</taxonomicName>
was located in an independent branch far from other species of
<taxonomicName id="192100E31BB1A51AEE6637629E8E75B1" authorityName="Nitschke &amp; Pyrenomyc.Germ" authorityYear="1870" class="Ascomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe" order="Diaporthales" pageId="0" pageNumber="319" phylum="Ascomycota" rank="genus">
<emphasis id="73BBF7C970877CD0DA9AF54E1E8B6E9F" italics="true" pageId="0" pageNumber="319">Diaporthe</emphasis>
</taxonomicName>
(Fig.
<figureCitation id="543F7BF779529D9EFA6E5AE4D7DD6FAB" captionStart="Figure 3" captionStartId="F1" captionText="Figure 3. Maximum Likelihood phylogram obtained (lLn = - 10678.2613) from the combined ITS, cal, his 3, tef 1 and tub 2 sequences of our strain and related Diaporthe spp. Diaporthe amygdali CBS 126679 T and D. eres CBS 138594 T were used as outgroup. Bootstrap support values ≥ 70 / Bayesian posterior probability scores ≥ 0.95 are indicated along branches. Branch lengths are proportional to distance. Novelties and emended taxa are indicated in bold. Type material of the different species is indicated with T. Figure legend refers to nucleotide substitutions per site." figureDoi="10.3897/mycokeys.99.110043.figure3" httpUri="https://binary.pensoft.net/fig/925257" pageId="0" pageNumber="319">3</figureCitation>
). This species is characterized by the production of alpha, beta and gamma conidia, which were not observed in other species reported from Cameroon except of
<taxonomicName id="800A746DB7590947A7A18310000B9CB1" class="Ascomycetes" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="D. isoberliniae" order="Diaporthales" pageId="0" pageNumber="319" phylum="Ascomycota" rank="species" species="isoberliniae">
<emphasis id="7ED6104EDD0D2A00ADD2DA405100C404" italics="true" pageId="0" pageNumber="319">D. isoberliniae</emphasis>
</taxonomicName>
. This latter species differs from
<taxonomicName id="731757EC3AC3BE462C8913DC1DE01992" class="Ascomycetes" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="D. rauvolfiae" order="Diaporthales" pageId="0" pageNumber="319" phylum="Ascomycota" rank="species" species="rauvolfiae">
<emphasis id="19C4A761422C0A84A762402B207C562C" italics="true" pageId="0" pageNumber="319">D. rauvolfiae</emphasis>
</taxonomicName>
in the length of the conidiophores (13-42
<normalizedToken id="4C5CDD7DB29430C2F953662E3E1CE8D9" originalValue="μm">μm</normalizedToken>
in
<taxonomicName id="A9030524436DEE79655BF4EFDDE3CBFA" class="Ascomycetes" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="D. isoberliniae" order="Diaporthales" pageId="0" pageNumber="319" phylum="Ascomycota" rank="species" species="isoberliniae">
<emphasis id="08FF41C87055A22643EA8DD13E966049" italics="true" pageId="0" pageNumber="319">D. isoberliniae</emphasis>
</taxonomicName>
vs 9-19.5
<normalizedToken id="B80107F7CF8D4CE909B3272F1FFD9CD8" originalValue="μm">μm</normalizedToken>
in
<taxonomicName id="793DF2D0BE56B30168DC16870B311881" class="Ascomycetes" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="D. rauvolfiae" order="Diaporthales" pageId="0" pageNumber="319" phylum="Ascomycota" rank="species" species="rauvolfiae">
<emphasis id="17A048609CE64A5A15D6F8A8D4B15C9D" italics="true" pageId="0" pageNumber="319">D. rauvolfiae</emphasis>
</taxonomicName>
), beta conidia (11.5-27.5
<normalizedToken id="1296A9C410E58294C5EE9DFEA2094101" originalValue="μm">μm</normalizedToken>
in
<taxonomicName id="A64B1FC1117A58B1436409445D504ABF" class="Ascomycetes" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="D. isoberliniae" order="Diaporthales" pageId="0" pageNumber="319" phylum="Ascomycota" rank="species" species="isoberliniae">
<emphasis id="E689F8F9FE63D2F01D2961DB69D8BE52" italics="true" pageId="0" pageNumber="319">D. isoberliniae</emphasis>
</taxonomicName>
vs 20-36.5
<normalizedToken id="1D148F7EE23F56349BE8C0A871407262" originalValue="μm">μm</normalizedToken>
in
<taxonomicName id="A53AB7008A4805FC0AE6826014426862" class="Ascomycetes" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="D. rauvolfiae" order="Diaporthales" pageId="0" pageNumber="319" phylum="Ascomycota" rank="species" species="rauvolfiae">
<emphasis id="988C703D0DAD63C9CC49DC4ABD96FC47" italics="true" pageId="0" pageNumber="319">D. rauvolfiae</emphasis>
</taxonomicName>
) and gamma conidia (10-18.5(-21)
<normalizedToken id="660270BA6C9F51B0A25437463E6862AA" originalValue="μm">μm</normalizedToken>
in
<taxonomicName id="E2056CF1912441EDBFFB28FBA9A3F53A" class="Ascomycetes" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="D. isoberliniae" order="Diaporthales" pageId="0" pageNumber="319" phylum="Ascomycota" rank="species" species="isoberliniae">
<emphasis id="DB9A4C91EC401F8639C65B8D17D16CE6" italics="true" pageId="0" pageNumber="319">D. isoberliniae</emphasis>
</taxonomicName>
vs (8-)9-13
<normalizedToken id="81B84EC82E379A4787F8501656E158BC" originalValue="μm">μm</normalizedToken>
in
<taxonomicName id="8101157321D7DC177BED2C5316C32852" class="Ascomycetes" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="D. rauvolfiae" order="Diaporthales" pageId="0" pageNumber="319" phylum="Ascomycota" rank="species" species="rauvolfiae">
<emphasis id="CEE769F66F6B0738FF280BF98DA272F3" italics="true" pageId="0" pageNumber="319">D. rauvolfiae</emphasis>
</taxonomicName>
). Both species are not phylogenetically related (Fig.
<figureCitation id="461A36E8508A9F1944B3D39EBB337B5D" captionStart="Figure 3" captionStartId="F1" captionText="Figure 3. Maximum Likelihood phylogram obtained (lLn = - 10678.2613) from the combined ITS, cal, his 3, tef 1 and tub 2 sequences of our strain and related Diaporthe spp. Diaporthe amygdali CBS 126679 T and D. eres CBS 138594 T were used as outgroup. Bootstrap support values ≥ 70 / Bayesian posterior probability scores ≥ 0.95 are indicated along branches. Branch lengths are proportional to distance. Novelties and emended taxa are indicated in bold. Type material of the different species is indicated with T. Figure legend refers to nucleotide substitutions per site." figureDoi="10.3897/mycokeys.99.110043.figure3" httpUri="https://binary.pensoft.net/fig/925257" pageId="0" pageNumber="319">3</figureCitation>
).
</paragraph>
</subSubSection>
</treatment>
</document>