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<document id="8E822D1CF57E926A2F33A6FAA491B238" ID-DOI="10.37828/em.2023.70.12" ID-ISSN="2336-9744" ID-Zenodo-Dep="13247599" IM.bibliography_approvedBy="felipe" IM.illustrations_approvedBy="felipe" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.tables_requiresApprovalFor="GgImagineBatch" IM.taxonomicNames_approvedBy="felipe" IM.treatments_approvedBy="felipe" checkinTime="1722973029055" checkinUser="felipe" docAuthor="Pešić, Vladimir, Bańkowska, Aleksandra, Zawal, Andrzej, Rewicz, Tomasz &amp; Smit, Harry" docDate="2023" docId="03B9878EFFAEFFD0C6CDFA79FB8182A5" docLanguage="en" docName="EcolMontenegrina.70.101-115.pdf" docOrigin="Ecologica Montenegrina 70" docSource="http://dx.doi.org/10.37828/em.2023.70.12" docStyle="DocumentStyle:3485E46B0EDC64DBF2992EDE30BFB5AE.2:EcolMontenegrina.2017-.journal_article" docStyleId="3485E46B0EDC64DBF2992EDE30BFB5AE" docStyleName="EcolMontenegrina.2017-.journal_article" docStyleVersion="2" docTitle="Mideopsis roztoczensis Biesiadka &amp; Kowalik 1979" docType="treatment" docVersion="3" lastPageNumber="108" masterDocId="FF80FFF6FFA9FFD7C65AFFBFFFF2857F" masterDocTitle="Molecular diversity and species delimitation in the genus Mideopsis Neuman, 1880 in Europe (Acari, Hydrachnidia, Mideopsidae)" masterLastPageNumber="115" masterPageNumber="101" pageNumber="108" updateTime="1722982174528" updateUser="ExternalLinkService" zenodo-license-document="CC-BY-4.0">
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<mods:title id="50C94A7E5173375EA993DFAAAD268C9A">Molecular diversity and species delimitation in the genus Mideopsis Neuman, 1880 in Europe (Acari, Hydrachnidia, Mideopsidae)</mods:title>
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<mods:namePart id="ED08ADA7263CF55714A463FD7349702A">Pešić, Vladimir</mods:namePart>
<mods:affiliation id="E560237A70BCBA4E495A3AC0748238B1">Department of Biology, University of Montenegro, Cetinjski put b. b., 81000 Podgorica, Montenegro.</mods:affiliation>
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<mods:namePart id="CAEC20C6DE50A15AB9E98626200EAF4E">Bańkowska, Aleksandra</mods:namePart>
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<mods:affiliation id="9D6FE933FC486747CCD00D42A9003A2F">Institute of Biology, University of Szczecin, Wąska 13, 71 - 415 Szczecin, Poland; E-mail: aleksandra. bankowska @ usz. edu. pl; https: // orcid. org / 0000 - 0003 - 1037 - 0550</mods:affiliation>
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<mods:namePart id="FEBB04E9EF4F13BBBC364861C8E40133">Zawal, Andrzej</mods:namePart>
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<mods:affiliation id="0BC8E19F4F3B5544296BA958F7CCDDDD">Institute of Marine and Environmental Sciences, Center of Molecular Biology and Biotechnology, University of Szczecin, Wąska 13, 71 - 415 Szczecin, Poland. E-mail: andrzej. zawal @ usz. edu. pl; https: // orcid. org / 0000 - 0002 - 5838 - 6060 &amp; Department of Plant Protection, Faculty of Agriculture, University of Tehran, Karaj, Iran.</mods:affiliation>
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<mods:namePart id="FE62C744FBA49A39F33A6DF3593075C2">Rewicz, Tomasz</mods:namePart>
<mods:nameIdentifier id="0870020FE5017E0590ECA159B2174072" type="ORCID">0000-0002-2085-4973</mods:nameIdentifier>
<mods:affiliation id="6999AF737827AF474F6A74D8E4C93012">Department of Invertebrate Zoology and Hydrobiology, University of Lodz, Banacha 12 / 16, 90 - 237 Łódź, Poland. E-mail: tomasz. rewicz @ biol. uni. lodz. pl; https: // orcid. org / 0000 - 0002 - 2085 - 4973</mods:affiliation>
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<mods:namePart id="FDB74C7B405EA3D56A55882B0B02590E">Smit, Harry</mods:namePart>
<mods:nameIdentifier id="0C0ECE27D4D80CE0929B70BC7A89FC78" type="ORCID">0000-0002-0376-6808</mods:nameIdentifier>
<mods:affiliation id="C08FC1BB559CB5246025F7154AD4D713">Naturalis Biodiversity Center, P. O. Box 9517, 2300 RA Leiden, the Netherlands. E-mail: Harry. Smit @ naturalis. nl; https: // orcid. org / 0000 - 0002 - 0376 - 6808 * Corresponding author. E-mail: vladopesic @ gmail. com, https: // orcid. org / 0000 - 0002 - 9724 - 345 X</mods:affiliation>
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<subSubSection id="C30A6513FFAEFFD0C6CDFA79FCF4809F" box="[151,774,1478,1504]" pageId="7" pageNumber="108" type="nomenclature">
<paragraph id="8BAF3698FFAEFFD0C6CDFA79FCF4809F" blockId="7.[151,774,1478,1504]" box="[151,774,1478,1504]" pageId="7" pageNumber="108">
<heading id="D0E781F4FFAEFFD0C6CDFA79FCF4809F" box="[151,774,1478,1504]" centered="true" fontSize="11" level="2" pageId="7" pageNumber="108" reason="2">
<taxonomicName id="4C104D1BFFAEFFD0C6CDFA79FCF4809F" authority="Biesiadka &amp; Kowalik, 1979" authorityName="Biesiadka &amp; Kowalik" authorityYear="1979" box="[151,774,1478,1504]" class="Arachnida" family="Mideopsidae" genus="Mideopsis" kingdom="Animalia" order="Trombidiformes" pageId="7" pageNumber="108" phylum="Arthropoda" rank="species" species="roztoczensis">
<emphasis id="B964EA8AFFAEFFD0C6CDFA79FCF4809F" bold="true" box="[151,774,1478,1504]" pageId="7" pageNumber="108">
<emphasis id="B964EA8AFFAEFFD0C6CDFA79FE58809F" bold="true" box="[151,426,1478,1504]" italics="true" pageId="7" pageNumber="108">Mideopsis roztoczensis</emphasis>
<bibRefCitation id="EF814B69FFAEFFD0C7EBFA79FCF4809F" author="Biesiadka, E. &amp; Kowalik, W." box="[433,774,1478,1504]" pageId="7" pageNumber="108" pagination="695 - 702" refId="ref5178" refString="Biesiadka, E. &amp; Kowalik, W. (1979) A new species of Mideopsis Neuman (Hydrachnellae, Acari) from Poland. Bulletin of the Polish Academy of Sci ences. Biological Sciences, Cl. II, 26, 695 - 702." type="journal article" year="1979">Biesiadka &amp; Kowalik, 1979</bibRefCitation>
</emphasis>
</taxonomicName>
</heading>
</paragraph>
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<subSubSection id="C30A6513FFAEFFD0C6CDF9B6FB8182A5" pageId="7" pageNumber="108" type="discussion">
<paragraph id="8BAF3698FFAEFFD0C6CDF9B6FAB98391" blockId="7.[151,1437,1545,2010]" pageId="7" pageNumber="108">
<emphasis id="B964EA8AFFAEFFD0C6CDF9B6FEFB835C" bold="true" box="[151,265,1545,1571]" pageId="7" pageNumber="108">Remarks</emphasis>
<taxonomicName id="4C104D1BFFAEFFD0C761F9B6FDBC835C" authorityName="Biesiadka &amp; Kowalik" authorityYear="1979" box="[315,590,1545,1571]" class="Arachnida" family="Mideopsidae" genus="Mideopsis" kingdom="Animalia" order="Trombidiformes" pageId="7" pageNumber="108" phylum="Arthropoda" rank="species" species="roztoczensis">
<emphasis id="B964EA8AFFAEFFD0C761F9B6FDBC835C" box="[315,590,1545,1571]" italics="true" pageId="7" pageNumber="108">Mideopsis roztoczensis</emphasis>
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was originally described by
<bibRefCitation id="EF814B69FFAEFFD0C5F5F9B6FAFF835C" author="Biesiadka, E. &amp; Kowalik, W." box="[943,1293,1545,1571]" pageId="7" pageNumber="108" pagination="695 - 702" refId="ref5178" refString="Biesiadka, E. &amp; Kowalik, W. (1979) A new species of Mideopsis Neuman (Hydrachnellae, Acari) from Poland. Bulletin of the Polish Academy of Sci ences. Biological Sciences, Cl. II, 26, 695 - 702." type="journal article" year="1979">Biesiadka &amp; Kowalik (1979)</bibRefCitation>
from Szum River, Biała Łada River, Wieprz River and their tributaries in Roztocze area, east-central
<collectingCountry id="F3077608FFAEFFD0C29BF994FAE4833A" box="[1217,1302,1579,1605]" name="Poland" pageId="7" pageNumber="108">Poland</collectingCountry>
. After that, it has been reported from different parts of Europe (
<bibRefCitation id="EF814B69FFAEFFD0C557F9F2FC0C8318" author="Gerecke, R. &amp; Gledhill, T. &amp; Pesic, V. &amp; Smit, H." box="[781,1022,1613,1639]" pageId="7" pageNumber="108" pagination="1 - 429" refId="ref5274" refString="Gerecke, R., Gledhill, T., Pesic, V. &amp; Smit, H. (2016) Chelicerata: Acari III. In: Gerecke, R. (Ed), Susswasserfauna von Mitteleuropa, Bd. 7 / 2 - 3. Springer-Verlag Berlin, Heidelberg, pp. 1 - 429." type="book chapter" year="2016">
Gerecke
<emphasis id="B964EA8AFFAEFFD0C521F9F2FC428318" box="[891,944,1613,1639]" italics="true" pageId="7" pageNumber="108">et al</emphasis>
. 2016
</bibRefCitation>
) and
<collectingCountry id="F3077608FFAEFFD0C21EF9F2FB538318" box="[1092,1185,1613,1639]" name="Turkey" pageId="7" pageNumber="108">Türkiye</collectingCountry>
(
<bibRefCitation id="EF814B69FFAEFFD0C2E9F9F2FA628318" author="Pesic, V. &amp; Esen, Y. &amp; Gulle, P. &amp; Zawal, A. &amp; Saboori, A. &amp; Jovanovic, M. &amp; Bankowska, A. &amp; Smit, H." box="[1203,1424,1613,1639]" pageId="7" pageNumber="108" pagination="1393 - 1407" refId="ref6557" refString="Pesic, V., Esen, Y., Gulle, P., Zawal, A., Saboori, A., Jovanovic, M., Bankowska, A. &amp; Smit, H. (2022 a) New records of water mites from Turkey and Iran revealed by DNA barcoding, with the description of a new species (Acari, Hydrachnidia). Systematic &amp; Applied Acarology, 27 (7), 1393 - 1407. https: // doi. org / 10.11158 / saa. 27.7.7" type="journal article" year="2022">
Pešić
<emphasis id="B964EA8AFFAEFFD0C2A7F9F2FAC18318" box="[1277,1331,1613,1639]" italics="true" pageId="7" pageNumber="108">et al</emphasis>
. 2022a
</bibRefCitation>
). According to the original description by
<bibRefCitation id="EF814B69FFAEFFD0C42CF9D1FC3F83F7" author="Biesiadka, E. &amp; Kowalik, W." box="[630,973,1646,1672]" pageId="7" pageNumber="108" pagination="695 - 702" refId="ref5178" refString="Biesiadka, E. &amp; Kowalik, W. (1979) A new species of Mideopsis Neuman (Hydrachnellae, Acari) from Poland. Bulletin of the Polish Academy of Sci ences. Biological Sciences, Cl. II, 26, 695 - 702." type="journal article" year="1979">Biesiadka &amp; Kowalik (1979)</bibRefCitation>
,
<taxonomicName id="4C104D1BFFAEFFD0C583F9D1FB1B83F7" authorityName="Biesiadka &amp; Kowalik" authorityYear="1979" box="[985,1257,1646,1672]" class="Arachnida" family="Mideopsidae" genus="Mideopsis" kingdom="Animalia" order="Trombidiformes" pageId="7" pageNumber="108" phylum="Arthropoda" rank="species" species="roztoczensis">
<emphasis id="B964EA8AFFAEFFD0C583F9D1FB1B83F7" box="[985,1257,1646,1672]" italics="true" pageId="7" pageNumber="108">Mideopsis roztoczensis</emphasis>
</taxonomicName>
in the first line is characterized by a more elevated dorsal shield with distinctly visible anteriorly diverging lines of particularly faint fine porosity (
<figureCitation id="132B2A1DFFAEFFD0C44DF90DFD8B83B3" box="[535,633,1714,1740]" captionStart="Figure 3" captionStartId="10.[151,227,1766,1789]" captionTargetBox="[200,1384,193,1746]" captionTargetId="figure-10@10.[199,1388,189,1748]" captionTargetPageId="10" captionText="Figure 3. A-D Photographs of dorsal shield: A M. roztoczensis, ♂, CCDB38233 C11, River Zeta, Montenegro. B M. orbicularis Clade 1, ♂, River Bukowa, Poland. C M. orbicularis Clade 2, ♀, RMNH.ACA.373, Netherlands. D M. orbicularis, ♂, Tjeukemeer, Netherlands. E-H Genital field: E M. roztoczensis, ♂, CCDB38233 C11, Montenegro. F-G M. orbicularis Clade 1, ♂: F River Bukowa, Poland; G RMNH.ACA.374, Netherlands. H-I M. orbicularis Clade 2, ♀, RMNH.ACA.373, Netherlands. K-O Ejaculatory complex. K M. roztoczensis, ♂, Roztocze, Poland (from Biesiadka &amp; Kowalik 1979: fig. 10), inset: photograph of anterior ramus, CCDB38233 C11, Montenegro). L M. orbicularis Clade 1, Roztocze, Poland (from Biesiadka &amp; Kowalik 1979: fig. 12). M M. orbicularis Clade 1, ♂, River Bukowa, Poland (inset: photograph of anterior ramus). N M. milankovici, paratype ♂, Montenegro (from Pešić &amp; Smit 2020). O M. orbicularis, ♂, Tjeukemeer, Netherlands. Scale bars = 100 µm (for K-L = 50 µm)." figureDoi="http://doi.org/10.5281/zenodo.13247605" httpUri="https://zenodo.org/record/13247605/files/figure.png" pageId="7" pageNumber="108">Fig. 3A</figureCitation>
), and the shape of the male ejaculatory complex with the wedge-shaped anterior ramus being wider, with a characteristic arrow-shaped delimited area - see
<figureCitation id="132B2A1DFFAEFFD0C298F96BFACF8391" box="[1218,1341,1748,1774]" captionStart="Figure 3" captionStartId="10.[151,227,1766,1789]" captionTargetBox="[200,1384,193,1746]" captionTargetId="figure-10@10.[199,1388,189,1748]" captionTargetPageId="10" captionText="Figure 3. A-D Photographs of dorsal shield: A M. roztoczensis, ♂, CCDB38233 C11, River Zeta, Montenegro. B M. orbicularis Clade 1, ♂, River Bukowa, Poland. C M. orbicularis Clade 2, ♀, RMNH.ACA.373, Netherlands. D M. orbicularis, ♂, Tjeukemeer, Netherlands. E-H Genital field: E M. roztoczensis, ♂, CCDB38233 C11, Montenegro. F-G M. orbicularis Clade 1, ♂: F River Bukowa, Poland; G RMNH.ACA.374, Netherlands. H-I M. orbicularis Clade 2, ♀, RMNH.ACA.373, Netherlands. K-O Ejaculatory complex. K M. roztoczensis, ♂, Roztocze, Poland (from Biesiadka &amp; Kowalik 1979: fig. 10), inset: photograph of anterior ramus, CCDB38233 C11, Montenegro). L M. orbicularis Clade 1, Roztocze, Poland (from Biesiadka &amp; Kowalik 1979: fig. 12). M M. orbicularis Clade 1, ♂, River Bukowa, Poland (inset: photograph of anterior ramus). N M. milankovici, paratype ♂, Montenegro (from Pešić &amp; Smit 2020). O M. orbicularis, ♂, Tjeukemeer, Netherlands. Scale bars = 100 µm (for K-L = 50 µm)." figureDoi="http://doi.org/10.5281/zenodo.13247605" httpUri="https://zenodo.org/record/13247605/files/figure.png" pageId="7" pageNumber="108">Figure 3K</figureCitation>
).
</paragraph>
<paragraph id="8BAF3698FFAEFFD0C6ADF94AFB8182A5" blockId="7.[151,1437,1545,2010]" pageId="7" pageNumber="108">
In the analysed material,
<taxonomicName id="4C104D1BFFAEFFD0C474F94AFD1B8270" authorityName="Biesiadka &amp; Kowalik" authorityYear="1979" box="[558,745,1781,1807]" class="Arachnida" family="Mideopsidae" genus="Mideopsis" kingdom="Animalia" order="Trombidiformes" pageId="7" pageNumber="108" phylum="Arthropoda" rank="species" species="roztoczensis">
<emphasis id="B964EA8AFFAEFFD0C474F94AFD1B8270" box="[558,745,1781,1807]" italics="true" pageId="7" pageNumber="108">M. roztoczensis</emphasis>
</taxonomicName>
was represented by 21 barcoded individuals assigned to four BINs (
<figureCitation id="132B2A1DFFAEFFD0C744F8A8FE95824E" box="[286,359,1815,1841]" captionStart="Figure 2" captionStartId="9.[151,227,1922,1945]" captionTargetBox="[151,1435,854,1903]" captionTargetId="figure-245@9.[151,1435,854,1903]" captionTargetPageId="9" captionText="Figure 2. Minimum Spanning Network showing phylogenetic relationships within three Mideopsis clades: M. roztoczensis, M. orbicularis Clade 1, and M. orbicularis Clade 2. Each bar represents a single mutational change, and a dashed line encircled by separate BINs. The diameter of the circles is proportional to the number of individuals in each haplotype sampled (see open circles with numbers)." figureDoi="http://doi.org/10.5281/zenodo.13247603" httpUri="https://zenodo.org/record/13247603/files/figure.png" pageId="7" pageNumber="108">Fig. 2</figureCitation>
). BIN ACI1492, which is the most widespread in Europe, contained most of the sequences from
<collectingCountry id="F3077608FFAEFFD0C683F886FE9A822C" box="[217,360,1849,1875]" name="Serbia" pageId="7" pageNumber="108">Montenegro</collectingCountry>
(n = 5),
<collectingCountry id="F3077608FFAEFFD0C78EF886FDC1822C" box="[468,563,1849,1875]" name="Norway" pageId="7" pageNumber="108">Norway</collectingCountry>
(n = 5),
<collectingCountry id="F3077608FFAEFFD0C4C4F886FD1E822C" box="[670,748,1849,1875]" name="Russia" pageId="7" pageNumber="108">Russia</collectingCountry>
(n =1),
<collectingCountry id="F3077608FFAEFFD0C515F886FB80822C" box="[847,1138,1849,1875]" name="Bosnia and Herzegovina" pageId="7" pageNumber="108">Bosnia and Herzegovina</collectingCountry>
(n = 3),
<collectingCountry id="F3077608FFAEFFD0C287F886FAC8822C" box="[1245,1338,1849,1875]" name="Turkey" pageId="7" pageNumber="108">Türkiye</collectingCountry>
(n = 4),
<collectingCountry id="F3077608FFAEFFD0C6CDF8E4FF10820A" box="[151,226,1883,1909]" name="Serbia" pageId="7" pageNumber="108">Serbia</collectingCountry>
(n = 1), and
<collectingCountry id="F3077608FFAEFFD0C727F8E4FE20820A" box="[381,466,1883,1909]" name="Cyprus" pageId="7" pageNumber="108">Cyprus</collectingCountry>
(n = 1). BIN AEN6785 consists of sequences from
<collectingCountry id="F3077608FFAEFFD0C263F8E4FB64820A" box="[1081,1174,1883,1909]" name="Turkey" pageId="7" pageNumber="108">Türkiye</collectingCountry>
,
<collectingCountry id="F3077608FFAEFFD0C2FFF8E4FB06820A" box="[1189,1268,1883,1909]" name="Serbia" pageId="7" pageNumber="108">Serbia</collectingCountry>
, and Northern Europe (
<collectingCountry id="F3077608FFAEFFD0C6A5F8C2FE9682E8" box="[255,356,1917,1943]" name="Norway" pageId="7" pageNumber="108">Norway</collectingCountry>
). The BIN AEA2936 includes sequences from
<collectingCountry id="F3077608FFAEFFD0C5F8F8C2FC1C82E8" box="[930,1006,1917,1943]" name="Serbia" pageId="7" pageNumber="108">Serbia</collectingCountry>
and two from
<collectingCountry id="F3077608FFAEFFD0C2FDF8C2FAFD82E8" box="[1191,1295,1917,1943]" name="Belgium" pageId="7" pageNumber="108">Belgium</collectingCountry>
, while BIN AEO2944 contains sequences from
<collectingCountry id="F3077608FFAEFFD0C415F821FD5B82C7" box="[591,681,1950,1976]" name="France" pageId="7" pageNumber="108">Corsica</collectingCountry>
deposited in BOLD. Some BINs, were found together at the same locality (e.g., BIN ACI1492 and BIN AEN
<quantity id="4CE89B7DFFAEFFD0C48EF87FFCDE82A5" box="[724,812,1984,2010]" metricMagnitude="2" metricUnit="m" metricValue="1.7233899999999998" pageId="7" pageNumber="108" unit="in" value="6785.0">6785 in</quantity>
Katušnica River in
<collectingCountry id="F3077608FFAEFFD0C24DF87FFB9582A5" box="[1047,1127,1984,2010]" name="Serbia" pageId="7" pageNumber="108">Serbia</collectingCountry>
).
</paragraph>
</subSubSection>
</treatment>
</document>