266 lines
18 KiB
XML
266 lines
18 KiB
XML
<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.77.59852" ID-GBIF-Dataset="78bab002-8c3e-4548-8377-cb082fa11d13" ID-PMC="PMC7819953" ID-Pensoft-Pub="1314-4049-77-65" ID-Pensoft-UUID="5D8D5A6329A4584ABC11C425E4B0E47A" ID-PubMed="33519269" ModsDocID="1314-4049-77-65" checkinTime="1610662561303" checkinUser="pensoft" docAuthor="Sun, Wenxiu, Huang, Shengting, Xia, Jiwen, Zhang, Xiuguo & Li, Zhuang" docDate="2021" docId="8658054EC61B5A399B67DB151DC5ECE2" docLanguage="en" docName="MycoKeys 77: 65-95" docOrigin="MycoKeys 77" docPubDate="2021-01-14" docSource="http://dx.doi.org/10.3897/mycokeys.77.59852" docTitle="Diaporthe pungensis S. T. Huang, J. W. Xia, X. G. Zhang, Z. Li 2021, sp. nov." docType="treatment" docVersion="4" id="5D8D5A6329A4584ABC11C425E4B0E47A" lastPageNumber="65" masterDocId="5D8D5A6329A4584ABC11C425E4B0E47A" masterDocTitle="Morphological and molecular identification of Diaporthe species in south-western China, with description of eight new species" masterLastPageNumber="95" masterPageNumber="65" pageNumber="65" updateTime="1668136608950" updateUser="ExternalLinkService">
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<mods:mods xmlns:mods="http://www.loc.gov/mods/v3">
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<mods:titleInfo>
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<mods:title>Morphological and molecular identification of Diaporthe species in south-western China, with description of eight new species</mods:title>
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</mods:titleInfo>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Sun, Wenxiu</mods:namePart>
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<mods:affiliation>College of Life Sciences, Yangtze University, Jingzhou 434025, Hubei, China</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Huang, Shengting</mods:namePart>
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<mods:affiliation>College of Life Sciences, Yangtze University, Jingzhou 434025, Hubei, China</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Xia, Jiwen</mods:namePart>
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<mods:nameIdentifier type="ORCID">https://orcid.org/0000-0002-7436-7249</mods:nameIdentifier>
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<mods:affiliation>Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Taian, Shandong, 271018, China</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Zhang, Xiuguo</mods:namePart>
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<mods:affiliation>Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Taian, Shandong, 271018, China</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Li, Zhuang</mods:namePart>
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<mods:affiliation>Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Taian, Shandong, 271018, China</mods:affiliation>
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<mods:nameIdentifier type="email">junwuxue@126.com</mods:nameIdentifier>
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</mods:name>
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<mods:typeOfResource>text</mods:typeOfResource>
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<mods:relatedItem type="host">
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<mods:titleInfo>
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<mods:title>MycoKeys</mods:title>
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</mods:titleInfo>
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<mods:part>
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<mods:date>2021</mods:date>
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<mods:detail type="pubDate">
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<mods:number>2021-01-14</mods:number>
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</mods:detail>
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<mods:detail type="volume">
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<mods:number>77</mods:number>
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</mods:detail>
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<mods:extent unit="page">
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<mods:start>65</mods:start>
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<mods:end>95</mods:end>
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</mods:extent>
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</mods:part>
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</mods:relatedItem>
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<mods:location>
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<mods:url>http://dx.doi.org/10.3897/mycokeys.77.59852</mods:url>
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</mods:location>
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<mods:classification>journal article</mods:classification>
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<mods:identifier type="DOI">http://dx.doi.org/10.3897/mycokeys.77.59852</mods:identifier>
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<mods:identifier type="Pensoft-Pub">1314-4049-77-65</mods:identifier>
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<mods:identifier type="Pensoft-UUID">5D8D5A6329A4584ABC11C425E4B0E47A</mods:identifier>
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</mods:mods>
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<treatment ID-GBIF-Taxon="176584077" LSID="urn:lsid:plazi:treatment:8658054EC61B5A399B67DB151DC5ECE2" httpUri="http://treatment.plazi.org/id/8658054EC61B5A399B67DB151DC5ECE2" lastPageNumber="65" pageId="0" pageNumber="65">
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<subSubSection pageId="0" pageNumber="65" type="nomenclature">
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<paragraph pageId="0" pageNumber="65">
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<taxonomicName LSID="8658054E-C61B-5A39-9B67-DB151DC5ECE2" authority="S. T. Huang, J. W. Xia, X. G. Zhang, Z. Li" authorityName="S. T. Huang, J. W. Xia, X. G. Zhang, Z. Li" authorityYear="2021" class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe pungensis" order="Diaporthales" pageId="0" pageNumber="65" phylum="Ascomycota" rank="species" species="pungensis" status="sp. nov.">Diaporthe pungensis S.T. Huang, J.W. Xia, X.G. Zhang, Z. Li</taxonomicName>
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<taxonomicNameLabel pageId="0" pageNumber="65">sp. nov.</taxonomicNameLabel>
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<figureCitation captionStart="Figure 9" captionStartId="F10" captionText="Figure 9. Diaporthe pungensis (SAUCC 194.112) a leaf of host plant b, c surface (b) and reverse (c) sides of colony after incubation for 15 days on PDA d conidiomata on PDA e-h conidiophores and conidiogenous cells i, l beta conidia j, k alpha conidia and beta conidia. Scale bars: 10 μm (e-l)." figureDoi="10.3897/mycokeys.77.59852.figure9" httpUri="https://binary.pensoft.net/fig/497512" pageId="0" pageNumber="65">Figure 9</figureCitation>
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="65" type="etymology">
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<paragraph pageId="0" pageNumber="65">Etymology.</paragraph>
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<paragraph pageId="0" pageNumber="65">
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Named after the host
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<taxonomicName class="Dicotyledoneae" family="Elaeagnaceae" genus="Elaeagnus" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="Elaeagnus pungens" order="Thymelaeales" pageId="0" pageNumber="65" phylum="Angiospermae" rank="species" species="pungens">
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<emphasis italics="true" pageId="0" pageNumber="65">Elaeagnus pungens</emphasis>
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</taxonomicName>
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on which it was collected.
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="65" type="diagnosis">
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<paragraph pageId="0" pageNumber="65">Diagnosis.</paragraph>
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<paragraph pageId="0" pageNumber="65">
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<taxonomicName authorityName="S. T. Huang, J. W. Xia, X. G. Zhang, Z. Li" authorityYear="2021" class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe pungensis" order="Diaporthales" pageId="0" pageNumber="65" phylum="Ascomycota" rank="species" species="pungensis">
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<emphasis italics="true" pageId="0" pageNumber="65">Diaporthe pungensis</emphasis>
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</taxonomicName>
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differs from its closest phylogenetic species
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<taxonomicName lsidName="D. inconspicua" pageId="0" pageNumber="65" rank="species" species="inconspicua">
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<emphasis italics="true" pageId="0" pageNumber="65">D. inconspicua</emphasis>
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</taxonomicName>
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R.R. Gomes et al. and
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<taxonomicName lsidName="D. poincianellae" pageId="0" pageNumber="65" rank="species" species="poincianellae">
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<emphasis italics="true" pageId="0" pageNumber="65">D. poincianellae</emphasis>
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</taxonomicName>
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T.G.L. Oloveira et al. in ITS,
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<emphasis italics="true" pageId="0" pageNumber="65">TUB</emphasis>
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,
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<emphasis italics="true" pageId="0" pageNumber="65">TEF</emphasis>
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,
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<emphasis italics="true" pageId="0" pageNumber="65">CAL</emphasis>
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and
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<emphasis italics="true" pageId="0" pageNumber="65">HIS</emphasis>
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loci based on the alignments deposited in Tree-BASE.
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</paragraph>
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<caption doi="10.3897/mycokeys.77.59852.figure9" httpUri="https://binary.pensoft.net/fig/497512" pageId="0" pageNumber="65" start="Figure 9" startId="F10">
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<paragraph pageId="0" pageNumber="65">
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<emphasis bold="true" pageId="0" pageNumber="65">Figure 9.</emphasis>
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<taxonomicName authorityName="S. T. Huang, J. W. Xia, X. G. Zhang, Z. Li" authorityYear="2021" class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe pungensis" order="Diaporthales" pageId="0" pageNumber="65" phylum="Ascomycota" rank="species" species="pungensis">
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<emphasis italics="true" pageId="0" pageNumber="65">Diaporthe pungensis</emphasis>
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</taxonomicName>
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(SAUCC194.112)
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<emphasis bold="true" pageId="0" pageNumber="65">a</emphasis>
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leaf of host plant
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<emphasis bold="true" pageId="0" pageNumber="65">b, c</emphasis>
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surface (
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<emphasis bold="true" pageId="0" pageNumber="65">b</emphasis>
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) and reverse (
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<emphasis bold="true" pageId="0" pageNumber="65">c</emphasis>
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) sides of colony after incubation for 15 days on PDA
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<emphasis bold="true" pageId="0" pageNumber="65">d</emphasis>
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conidiomata on PDA
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<emphasis bold="true" pageId="0" pageNumber="65">e-h</emphasis>
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conidiophores and conidiogenous cells
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<emphasis bold="true" pageId="0" pageNumber="65">i, l</emphasis>
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beta conidia
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<emphasis bold="true" pageId="0" pageNumber="65">j, k</emphasis>
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alpha conidia and beta conidia. Scale bars: 10
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<normalizedToken originalValue="μm">μm</normalizedToken>
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(
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<emphasis bold="true" pageId="0" pageNumber="65">e-l</emphasis>
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).
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</paragraph>
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</caption>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="65" type="materials_examined">
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<paragraph pageId="0" pageNumber="65">Type.</paragraph>
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<paragraph pageId="0" pageNumber="65">
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China, Yunnan Province: Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, on diseased leaves of
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<taxonomicName class="Dicotyledoneae" family="Elaeagnaceae" genus="Elaeagnus" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="Elaeagnus pungens" order="Thymelaeales" pageId="0" pageNumber="65" phylum="Angiospermae" rank="species" species="pungens">
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<emphasis italics="true" pageId="0" pageNumber="65">Elaeagnus pungens</emphasis>
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</taxonomicName>
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. 19 April 2019, S.T. Huang, HSAUP194.112, holotype, ex-holotype living culture SAUCC194.112.
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="65" type="description">
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<paragraph pageId="0" pageNumber="65">Description.</paragraph>
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<paragraph pageId="0" pageNumber="65">
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Asexual morph: Conidiomata pycnidial, 3-5 pycnidia grouped together, superficial to embedded on PDA, erumpent, thin-walled, dark brown to black, globose or subglobose, exuding white creamy conidial mass from the ostioles. Conidiophores hyaline, aseptate, cylindrical, smooth, straight to sinuous, unbranched, 11.0-14.5
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<normalizedToken originalValue="×">x</normalizedToken>
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1.5-2.3
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<normalizedToken originalValue="µm">µm</normalizedToken>
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. Conidiogenous cells phialidic, cylindrical, terminal, 8.0-9.5
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<normalizedToken originalValue="×">x</normalizedToken>
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1.0-2.5
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<normalizedToken originalValue="µm">µm</normalizedToken>
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. Alpha conidia, hyaline, smooth, aseptate, ellipsoidal to fusoid, 2-3 guttulate, apex subobtuse, base subtruncate, 6.0-8.5
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<normalizedToken originalValue="×">x</normalizedToken>
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2.0-3.3
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<normalizedToken originalValue="µm">µm</normalizedToken>
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(mean = 6.6
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<normalizedToken originalValue="×">x</normalizedToken>
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2.5
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<normalizedToken originalValue="μm">μm</normalizedToken>
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, n = 20). Beta conidia hyaline, aseptate, eguttulate, filiform, slightly curved, tapering towards the apex, base truncate, some conidia are in the immature stage swollen in the middle, 24.0-28.9
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<normalizedToken originalValue="×">x</normalizedToken>
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1.0-2.0
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<normalizedToken originalValue="µm">µm</normalizedToken>
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(mean = 26.9
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<normalizedToken originalValue="×">x</normalizedToken>
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1.4
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<normalizedToken originalValue="μm">μm</normalizedToken>
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, n = 20). Gamma conidia not observed, sexual morph not observed.
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="65" type="description">
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<paragraph pageId="0" pageNumber="65">Culture characteristics.</paragraph>
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<paragraph pageId="0" pageNumber="65">Pure culture was isolated by subbing hyphal tips growing from surface sterilized plant material. Colonies on PDA cover the 3/4 of Petri dish diameter after incubation for 15 days in dark conditions at 25 °C, flat, cottony in the center with medium developed aerial mycelium, sparse in the outer region. With several concentric rings of dense and sparse hyphae, irregular margin, white on surface side, white to pale yellow and cinnamon speckle on reverse side.</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="65" type="additional specimen examined">
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<paragraph pageId="0" pageNumber="65">Additional specimen examined.</paragraph>
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<paragraph pageId="0" pageNumber="65">
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China, Yunnan Province: Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, on infected leaves of
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<taxonomicName class="Dicotyledoneae" family="Theaceae" genus="Camellia" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="Camellia sinensis" order="Guttiferales" pageId="0" pageNumber="65" phylum="Angiospermae" rank="species" species="sinensis">
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<emphasis italics="true" pageId="0" pageNumber="65">Camellia sinensis</emphasis>
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</taxonomicName>
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. 19 April 2019, S.T. Huang, HSAUP194.89 paratype, living culture SAUCC194.89.
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="65" type="notes">
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<paragraph pageId="0" pageNumber="65">Notes.</paragraph>
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<paragraph pageId="0" pageNumber="65">
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<taxonomicName authorityName="S. T. Huang, J. W. Xia, X. G. Zhang, Z. Li" authorityYear="2021" class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe pungensis" order="Diaporthales" pageId="0" pageNumber="65" phylum="Ascomycota" rank="species" species="pungensis">
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<emphasis italics="true" pageId="0" pageNumber="65">Diaporthe pungensis</emphasis>
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</taxonomicName>
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forms a distinct clade with high support (ML/BI = 100/1), and differed with the closely related species (
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<taxonomicName lsidName="D. inconspicua" pageId="0" pageNumber="65" rank="species" species="inconspicua">
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<emphasis italics="true" pageId="0" pageNumber="65">D. inconspicua</emphasis>
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</taxonomicName>
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and
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<taxonomicName lsidName="D. poincianellae" pageId="0" pageNumber="65" rank="species" species="poincianellae">
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<emphasis italics="true" pageId="0" pageNumber="65">D. poincianellae</emphasis>
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</taxonomicName>
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) on ITS,
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<emphasis italics="true" pageId="0" pageNumber="65">TUB</emphasis>
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,
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<emphasis italics="true" pageId="0" pageNumber="65">CAL</emphasis>
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and
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<emphasis italics="true" pageId="0" pageNumber="65">HIS</emphasis>
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loci (94% in ITS, 92% in
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<emphasis italics="true" pageId="0" pageNumber="65">TUB</emphasis>
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, 70% in
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<emphasis italics="true" pageId="0" pageNumber="65">TEF</emphasis>
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, 92% in
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<emphasis italics="true" pageId="0" pageNumber="65">CAL</emphasis>
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and 92% in
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<emphasis italics="true" pageId="0" pageNumber="65">HIS</emphasis>
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; and 95% in ITS, 94% in
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<emphasis italics="true" pageId="0" pageNumber="65">TUB</emphasis>
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, 80% in
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<emphasis italics="true" pageId="0" pageNumber="65">TEF</emphasis>
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, 94% in
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<emphasis italics="true" pageId="0" pageNumber="65">CAL</emphasis>
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and 89% in
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<emphasis italics="true" pageId="0" pageNumber="65">HIS</emphasis>
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, respectively). Moreover,
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<taxonomicName authorityName="S. T. Huang, J. W. Xia, X. G. Zhang, Z. Li" authorityYear="2021" class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe pungensis" order="Diaporthales" pageId="0" pageNumber="65" phylum="Ascomycota" rank="species" species="pungensis">
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<emphasis italics="true" pageId="0" pageNumber="65">Diaporthe pungensis</emphasis>
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</taxonomicName>
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differs from
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<taxonomicName lsidName="D. inconspicua" pageId="0" pageNumber="65" rank="species" species="inconspicua">
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<emphasis italics="true" pageId="0" pageNumber="65">D. inconspicua</emphasis>
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</taxonomicName>
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, in having guttulate of alpha conidia, and having larger alpha conidia (6.0-8.5
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<normalizedToken originalValue="×">x</normalizedToken>
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2.0-3.3 vs. 5.5-6.5
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<normalizedToken originalValue="×">x</normalizedToken>
|
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1.5-2
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<normalizedToken originalValue="μm">μm</normalizedToken>
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) (
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<bibRefCitation DOI="https://doi.org/10.1590/0102-33062018abb0154" author="Bezerra, JDP" journalOrPublisher="Acta Botanica Brasilica" pageId="0" pageNumber="65" pagination="656 - 666" refId="B1" refString="Bezerra, JDP, Machado, AR, Firmino, AL, Rosado, AWC, de Souza, CAF, de Souza-Motta, CM, de Sousa Freire, KTL, Paiva, LM, Magalhaes, OMC, Pereira, OL, 2018. Mycological diversity description I. Acta Botanica Brasilica 32 (4): 656 - 666, DOI: https://doi.org/10.1590/0102-33062018abb0154" title="Mycological diversity description I." url="https://doi.org/10.1590/0102-33062018abb0154" volume="32" year="2018">Bezerra et al. 2018</bibRefCitation>
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). Furthermore,
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<taxonomicName authorityName="S. T. Huang, J. W. Xia, X. G. Zhang, Z. Li" authorityYear="2021" class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe pungensis" order="Diaporthales" pageId="0" pageNumber="65" phylum="Ascomycota" rank="species" species="pungensis">
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<emphasis italics="true" pageId="0" pageNumber="65">Diaporthe pungensis</emphasis>
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</taxonomicName>
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||
can produce two types of conidia (α-conidia and
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<normalizedToken originalValue="β-conidia">β-conidia</normalizedToken>
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), but
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<taxonomicName lsidName="D. poincianellae" pageId="0" pageNumber="65" rank="species" species="poincianellae">
|
||
<emphasis italics="true" pageId="0" pageNumber="65">D. poincianellae</emphasis>
|
||
</taxonomicName>
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||
only produce a
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<normalizedToken originalValue="α-conidia">α-conidia</normalizedToken>
|
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(
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<bibRefCitation author="Crous, PW" journalOrPublisher="Persoonia" pageId="0" pageNumber="65" pagination="238 - 417" refId="B11" refString="Crous, PW, Luangsa-ard, JJ, Wingfield, MJ, Carnegie, AJ, Hernandez-Restrepo, M, Lombard, L, Roux, J, Barreto, RW, Baseia, IG, Cano-Lira, JF, 2018b. Fungal planet description sheets: 785-867. Persoonia 41 (1): 238 - 417" title="Fungal planet description sheets: 785 - 867." volume="41" year="2018 b">Crous et al. 2018b</bibRefCitation>
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).
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</paragraph>
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</subSubSection>
|
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</treatment>
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||
</document> |