509 lines
126 KiB
XML
509 lines
126 KiB
XML
<document id="84AA648E0C3D4323E39E391A911B26EE" ID-CLB-Dataset="267791" ID-DOI="10.11646/zootaxa.5343.5.2" ID-GBIF-Dataset="467a16a3-268b-4241-a6c2-77da2c3f4eb2" ID-ISSN="1175-5326" ID-Zenodo-Dep="8345479" ID-ZooBank="2E48E826-9DB3-45C3-B231-CBB84B725119" IM.bibliography_approvedBy="julia" IM.illustrations_approvedBy="julia" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.tables_approvedBy="julia" IM.taxonomicNames_approvedBy="julia" IM.treatments_approvedBy="julia" checkinTime="1694702795525" checkinUser="plazi" docAuthor="Neuhaus, Birger, Lu, Borong, Yamasaki, Hiroshi & González-Casarrubios, Alberto" docDate="2023" docId="F65487889905FF9AFF33FB797164F891" docLanguage="en" docName="zootaxa.5343.5.2.pdf" docOrigin="Zootaxa 5343 (5)" docSource="https://www.mapress.com/zt/article/download/zootaxa.5343.5.2/51843" docStyle="DocumentStyle:647186512141C8FC8976D5BCC54AEB7D.9:Zootaxa.2013-.journal_article" docStyleId="647186512141C8FC8976D5BCC54AEB7D" docStyleName="Zootaxa.2013-.journal_article" docStyleVersion="9" docTitle="Trematosoma husselae Neuhaus & Lu & Yamasaki & González-Casarrubios 2023, sp. nov." docType="treatment" docVersion="2" lastPageNumber="448" masterDocId="0A6DFFF09903FF93FFA4FFB67510FF97" masterDocTitle="Epibiontic life on intertidal Setaphyes kielensis and S. dentatus (Kinorhyncha, Pycnophyidae) from Sylt, North Sea, Germany, with a description of a new species of Trematosoma (Ciliophora, Acinetidae) and a redescription of Cothurnia buetschlii (Ciliophora, Vaginicolidae)" masterLastPageNumber="470" masterPageNumber="439" pageNumber="445" updateTime="1694787949847" updateUser="ExternalLinkService" zenodo-license-document="CLOSED">
|
||
<mods:mods id="CE809B82F274DC0433B1BD35F2E7744B" xmlns:mods="http://www.loc.gov/mods/v3">
|
||
<mods:titleInfo id="919BB8217558E430297CFA9C11FB1C04">
|
||
<mods:title id="9C2E929FCD9FD970673AA49784BB98F6">Epibiontic life on intertidal Setaphyes kielensis and S. dentatus (Kinorhyncha, Pycnophyidae) from Sylt, North Sea, Germany, with a description of a new species of Trematosoma (Ciliophora, Acinetidae) and a redescription of Cothurnia buetschlii (Ciliophora, Vaginicolidae)</mods:title>
|
||
</mods:titleInfo>
|
||
<mods:name id="A2328493DAC4C871BB0B38CF482FBC7C" type="personal">
|
||
<mods:role id="2EF486B9995294FAF4B1FD52EA543514">
|
||
<mods:roleTerm id="F27DA0D4D26A8CC5C850064960B5F75E">Author</mods:roleTerm>
|
||
</mods:role>
|
||
<mods:namePart id="326686069AEA44BABD5E47E4FB3C6388">Neuhaus, Birger</mods:namePart>
|
||
<mods:nameIdentifier id="A069A33DCB959B6CFB5FB9BE00B15C62" type="ORCID">0000-0002-5794-6409</mods:nameIdentifier>
|
||
<mods:nameIdentifier id="FB95314CA2166C055CA898E19D0039D4" type="email">birger.neuhaus@mfn.berlin</mods:nameIdentifier>
|
||
</mods:name>
|
||
<mods:name id="2E6DC24A5BA171A4605C5EAFFF79B474" type="personal">
|
||
<mods:role id="A495DA8B812FBB60C4138EF1C4316BDF">
|
||
<mods:roleTerm id="0F868AF9F2C97994C96D4C6A6B86351A">Author</mods:roleTerm>
|
||
</mods:role>
|
||
<mods:namePart id="22579DFE31ADE7E461CE90346E579671">Lu, Borong</mods:namePart>
|
||
</mods:name>
|
||
<mods:name id="238217C2767C90A879475D4E5F8A2044" type="personal">
|
||
<mods:role id="3A390EA69CBE539367BF3C9F0792A7C5">
|
||
<mods:roleTerm id="29A2510FE9AF60414EF198F99FFB2402">Author</mods:roleTerm>
|
||
</mods:role>
|
||
<mods:namePart id="296EA505DA6D90B5A92CFCCC02F250BD">Yamasaki, Hiroshi</mods:namePart>
|
||
<mods:affiliation id="4A8927F1DB5FED9DBFFFE9AF48EE0B9A">Kyushu University, Faculty of Arts and Science, Center 3 goukan room 3310, Motooka 744, J- 819 - 0395, Fukuoka, Nishi-ku, Fukuoka city, Japan</mods:affiliation>
|
||
</mods:name>
|
||
<mods:name id="5533370B1850B5534ABD6751DBD86293" type="personal">
|
||
<mods:role id="0FFB6FCABFB202A403FBDA6450F62FFE">
|
||
<mods:roleTerm id="6A4748BE5D46108892792A32C8629A55">Author</mods:roleTerm>
|
||
</mods:role>
|
||
<mods:namePart id="8FADA832CEFE79AB80452F0A48FCE561">González-Casarrubios, Alberto</mods:namePart>
|
||
<mods:nameIdentifier id="1EE8264986533FBD0A35FF03CCF6D698" type="ORCID">0000-0002-5794-6409</mods:nameIdentifier>
|
||
<mods:affiliation id="9B76DA751A3A8393AF4481967F7A2373">Complutense University of Madrid (UCM), Faculty of Biology, Department of Biodiversity, Ecology and Evolution (BEE), Madrid, Spain</mods:affiliation>
|
||
<mods:nameIdentifier id="BB59519579FEC6590C3ED9A55E0EC5EB" type="email">birger.neuhaus@mfn.berlin</mods:nameIdentifier>
|
||
</mods:name>
|
||
<mods:typeOfResource id="3573623228E77DBCAA2BEBE0897A27E2">text</mods:typeOfResource>
|
||
<mods:relatedItem id="4275D3357649A3939F8EA6CBF6A702B0" type="host">
|
||
<mods:titleInfo id="BAF56EBCB4C540B49B095180F041217A">
|
||
<mods:title id="A816B8B5BE288707A15DF9E4FBB96DC7">Zootaxa</mods:title>
|
||
</mods:titleInfo>
|
||
<mods:part id="C509A9F119CC51AE6E60C79276318538">
|
||
<mods:date id="A1779FD6CFCF7021636AD31C2DC1C73C">2023</mods:date>
|
||
<mods:detail id="21426715E8EF5599D2FF102514F06018" type="pubDate">
|
||
<mods:number id="25492DCA9508CA15F1B470BA7BB5F225">2023-09-12</mods:number>
|
||
</mods:detail>
|
||
<mods:detail id="E0A490B781B2C5594A475BEB874B5241" type="volume">
|
||
<mods:number id="066FCDB188E2A85203DBDA161545D15D">5343</mods:number>
|
||
</mods:detail>
|
||
<mods:detail id="1283C24520DF9BD3C77813F893F3F4ED" type="issue">
|
||
<mods:number id="445F382836EBD97FB51F9424BB4AAB5C">5</mods:number>
|
||
</mods:detail>
|
||
<mods:extent id="E022C7933397DBFC03B94EC0A6311949" unit="page">
|
||
<mods:start id="131E4087A0814D95FB9182C185A684CC">439</mods:start>
|
||
<mods:end id="6AC3A70D8F335C280F11C56E6ADCFB95">470</mods:end>
|
||
</mods:extent>
|
||
</mods:part>
|
||
</mods:relatedItem>
|
||
<mods:location id="82CA9B9641E1432190C23ADB75696BEA">
|
||
<mods:url id="477BFD8E641E2F71D6DEDE254D4CC92C">https://www.mapress.com/zt/article/download/zootaxa.5343.5.2/51843</mods:url>
|
||
</mods:location>
|
||
<mods:classification id="1D149539B18D1DDBBCE9EE2708270623">journal article</mods:classification>
|
||
<mods:identifier id="1861A265173EFD9A19410C6441725FD4" type="CLB-Dataset">267791</mods:identifier>
|
||
<mods:identifier id="45D6756B2C9BD9B557347BAA6A047B0E" type="DOI">10.11646/zootaxa.5343.5.2</mods:identifier>
|
||
<mods:identifier id="C924D1F423F079353315BC7219C13F9E" type="GBIF-Dataset">467a16a3-268b-4241-a6c2-77da2c3f4eb2</mods:identifier>
|
||
<mods:identifier id="A310716E0A515D11866773379730D14A" type="ISSN">1175-5326</mods:identifier>
|
||
<mods:identifier id="8F2B1B7EF040D8ABD06A3F21FB564378" type="Zenodo-Dep">8345479</mods:identifier>
|
||
<mods:identifier id="7E1C55FEA56191EE2EEEBE78AFCD0C70" type="ZooBank">2E48E826-9DB3-45C3-B231-CBB84B725119</mods:identifier>
|
||
</mods:mods>
|
||
<treatment id="F65487889905FF9AFF33FB797164F891" ID-DOI="http://doi.org/10.5281/zenodo.8345499" ID-Zenodo-Dep="8345499" LSID="urn:lsid:plazi:treatment:F65487889905FF9AFF33FB797164F891" httpUri="http://treatment.plazi.org/id/F65487889905FF9AFF33FB797164F891" lastPageId="9" lastPageNumber="448" pageId="6" pageNumber="445">
|
||
<subSubSection id="36E765159905FF95FF33FB797701FB7E" box="[151,529,1231,1258]" pageId="6" pageNumber="445" type="nomenclature">
|
||
<paragraph id="7E42369E9905FF95FF33FB797701FB7E" blockId="6.[151,529,1231,1294]" box="[151,529,1231,1258]" pageId="6" pageNumber="445">
|
||
<heading id="250A81F29905FF95FF33FB797701FB7E" bold="true" box="[151,529,1231,1258]" fontSize="11" level="1" pageId="6" pageNumber="445" reason="1">
|
||
<emphasis id="4C89EA8C9905FF95FF33FB797701FB7E" bold="true" box="[151,529,1231,1258]" pageId="6" pageNumber="445">
|
||
<taxonomicName id="B9FD4D1D9905FF95FF33FB7974BCFB7D" authority="Neuhaus & Lu & Yamasaki & González-Casarrubios, 2023" authorityName="Neuhaus & Lu & Yamasaki & González-Casarrubios" authorityYear="2023" box="[151,428,1231,1258]" class="Kinetofragminophora" family="Acinetidae" genus="Trematosoma" higherTaxonomySource="GBIF" kingdom="Chromista" order="Suctorida" pageId="6" pageNumber="445" phylum="Ciliophora" rank="species" species="husselae" status="sp. nov.">
|
||
<emphasis id="4C89EA8C9905FF95FF33FB7974BCFB7D" bold="true" box="[151,428,1231,1258]" italics="true" pageId="6" pageNumber="445">Trematosoma husselae</emphasis>
|
||
</taxonomicName>
|
||
<taxonomicNameLabel id="57BA57F79905FF95FE17FB797701FB7E" box="[435,529,1231,1257]" pageId="6" pageNumber="445" rank="species">sp. nov.</taxonomicNameLabel>
|
||
</emphasis>
|
||
</heading>
|
||
</paragraph>
|
||
</subSubSection>
|
||
<subSubSection id="36E765159905FF95FF33FB45769EFA09" pageId="6" pageNumber="445" type="description">
|
||
<paragraph id="7E42369E9905FF95FF33FB4574F7FA9A" blockId="6.[151,529,1231,1294]" box="[151,487,1267,1294]" pageId="6" pageNumber="445">
|
||
(
|
||
<figureCitation id="E6C62A1B9905FF95FF3BFB457414FA99" box="[159,260,1267,1294]" captionStart-0="FIGURE 2" captionStart-1="FIGURE 3" captionStart-2="FIGURE 4" captionStart-3="FIGURE 5" captionStartId-0="7.[151,250,913,937]" captionStartId-1="8.[151,250,1750,1774]" captionStartId-2="10.[151,250,1749,1773]" captionStartId-3="12.[151,250,186,210]" captionTargetBox-0="[253,1340,184,889]" captionTargetBox-1="[151,1436,181,1726]" captionTargetBox-2="[152,1436,181,1725]" captionTargetId-0="figure-17@7.[248,1340,181,889]" captionTargetId-1="figure-24@8.[151,1436,181,1726]" captionTargetId-2="figure-24@10.[152,1436,181,1725]" captionTargetId-3="figure-17@11.[151,1436,181,1725]" captionTargetPageId-0="7" captionTargetPageId-1="8" captionTargetPageId-2="10" captionTargetPageId-3="11" captionText-0="FIGURE 2. Trematosoma husselae sp. nov. on Setaphyes kielensis. A. Drawing of the holotype with internal developing swarmer ventrally on segment 1 of basibiont (ZMB 12499). B. Paratype in side view ventrally on segment 3 of basibiont (ZMB 12489). Arrow marks apical cleft within lorica. Abbreviations: ca, basal cavity in lorica; lo, lorica; ma, macronucleus; mi, micronucleus; st, stalk with internal striation (information taken from different specimen); sw, swarmer; te, tentacle; zo, zooid. Scale bar 30 µm." captionText-1="FIGURE 3. Setaphyes kielensis (A–C, ZMB 12475; D, ZMB 12464; E, F, ZMB 12465; G, ZMB 12462) with specimens of Trematosoma husselae sp. nov., SEM, ventral view. A–C. Specimen with 43 epibionts, segments 1–4 (A), 5–7 (B), and 8–11 (C). D. Segment 8, epibiont surface. E. Segment 6, epibiont surface covered with detritus. F. Segment 8, frontal view on small spherical specimen of Trematosoma husselae sp. nov. G. Segment 10, arrow marks specimen with withdrawn tentacles. Abbreviations: gco, gland cell outlet; lts, lateral terminal spine; s, segment, followed by segment number; se, seta; su, suctorian epibiont; te, tentacle. Scale bar in A–C 40 µm, in D–F 10 µm, and in G 20 µm." captionText-2="FIGURE 4. Setaphyes kielensis (A, ZMB 12469; B, ZMB 12489; C, ZMB 12486; D, E, ZMB 12492; F, ZMB 12488) with specimens of Trematosoma husselae sp. nov., SEM (A), and DIC (B–F). A. Segment 1, empty shells of epibionts, ventral view. B. Segments 1–11, optical section. C. Epibionts with capitated tentacles arranged in left and right row. Extended focus image of three images. D. Epibiont with developing swarmer. Arrowheads mark membrane of swarmer. E. Epibiont with swarmer recently freed from its parent. F. Segments 4–6, ventral view. Note thickened lorica in basal two thirds of epibionts.Arrow marks longitudinal striation of stalk of epibiont. Abbreviations: ca, basal cavity in lorica; co, specimen of Cothurnia buetschlii; lo, lorica; lts, lateral terminal spine; ma, macronucleus; mi, micronucleus; s, segment, followed by segment number; su, suctorian epibiont; sw, swarmer; te, tentacle. Scale bar in A, D, E 20 µm, in B 100 µm, in C 30 µm, and in F 50 µm." captionText-3="FIGURE 5. Setaphyes kielensis (A, ZMB 12489; B, ZMB 12500; C, ZMB 12499; D, ZMB 12487; J, ZMB 12486; L, ZMB 12397; M, ZMB 12369) and S. dentatus (E–G, K, ZMB 12503; F, H, I, ZMB 12494) with specimens of Trematosoma husselae sp. nov. (A–G, J–M), Cothurnia buetschlii (J), and a case of fungus or developmental artefact (H, I, ZMB 12494), DIC. A. Segments 1–4, lateral and dorso-ventral view on the calyciform, dorso-ventrally compressed lorica with the zooid, ventrolateral view on basibiont. B. Epibiont with swarmer, stalk, and lorica in optical section. C. Epibiont with two macronuclei not fusing at any focal level. D, E. Young, small, spherical epibionts with few tentacles, not all tentacles in focus. F. Young specimen on cuticle of basibiont with striation pattern on surface (arrows). G. Epibiont with tentacles in empty shell of suctorian epibiont. H, I. Segment 10, dorsally (H) and optical section (I), subcuticular fungus or internal sclerotisation artefact of the cuticle (arrows). J. Assemblage of epibionts on segment 10 of basibiont with C. buetschlii (contracted specimen left, extended specimen right), T. husselae sp. nov., and two kinds of filamentous bacteria. Arrows mark endostyle. K. Suctorian on segment 7 of S. dentatus. L, M. Specimens collected 1998, showing cellular substructures (L) and withdrawn zooid with minimum amount of cellular substructures (M). Extended focus images of six (D), eight (H, L), and three (K) images. Arrowheads mark stalks in B, D, E, G, and J. Abbreviations: ba, bacteria; ca, basal cavity in lorica; co, C. buetschlii; en, endostyle; lo, lorica; ma, macronucleus; s, segment, followed by segment number; sc, spherical cavity; sp, sperm; st, stalk; su, suctorian epibiont; sw, swarmer; te, tentacle; tu, tube on segment 2. Scale bar in A 50 µm, in B 20 µm, valid for B, G–J and M, in C 20 µm, valid for C, K, and L, and in D–F 10 µm." figureDoi-0="http://doi.org/10.5281/zenodo.8345483" figureDoi-1="http://doi.org/10.5281/zenodo.8345485" figureDoi-2="http://doi.org/10.5281/zenodo.8345487" figureDoi-3="http://doi.org/10.5281/zenodo.8349223" httpUri-0="https://zenodo.org/record/8345483/files/figure.png" httpUri-1="https://zenodo.org/record/8345485/files/figure.png" httpUri-2="https://zenodo.org/record/8345487/files/figure.png" httpUri-3="https://zenodo.org/record/8349223/files/figure.png" pageId="6" pageNumber="445">Figs 2–5</figureCitation>
|
||
,
|
||
<figureCitation id="E6C62A1B9905FF95FEB4FB427430FA99" box="[272,288,1268,1294]" captionStart="FIGURE 7" captionStartId="14.[151,250,1739,1763]" captionTargetBox="[151,1436,181,1715]" captionTargetId="figure-24@14.[151,1436,181,1715]" captionTargetPageId="14" captionText="FIGURE 7. Setaphyes kielensis (ZMB 12468) with epibionts, SEM. A. Segments 1–11, ventral-right. B. Segments 4–8, ventral view. Arrows mark laterally recessed anterior margin of lorica of Cothurnia buetschlii. Abbreviations: co, Cothurnia buetschlii; fp, filamentous prokaryotes; lts, lateral terminal spine; s, segment, followed by segment number; se, seta; su, suctorian epibiont; tu, tube. Scale bar in A 100 µm and in B 20 µm." figureDoi="http://doi.org/10.5281/zenodo.8345491" httpUri="https://zenodo.org/record/8345491/files/figure.png" pageId="6" pageNumber="445">7</figureCitation>
|
||
,
|
||
<figureCitation id="E6C62A1B9905FF95FE88FB45742CFA9A" box="[300,316,1267,1293]" captionStart="FIGURE 8" captionStartId="15.[151,250,1745,1769]" captionTargetBox="[151,1436,182,1721]" captionTargetId="figure-17@15.[151,1436,181,1721]" captionTargetPageId="15" captionText="FIGURE 8. Setaphyes kielensis (A, ZMB 12468; B, ZMB 12474) with epibionts, SEM. A. Segment 6, left side. Arrow marks laterally recessed anterior margin of lorica of Cothurnia buetschlii. B. Segments 8–10, dorsal view.Abbreviations: co, Cothurnia buetschlii; fp, filamentous prokaryotes; s, segment, followed by segment number; su, suctorian epibiont; te, tentacles. Scale bar in A 10 µm and in B 20 µm." figureDoi="http://doi.org/10.5281/zenodo.8345493" httpUri="https://zenodo.org/record/8345493/files/figure.png" pageId="6" pageNumber="445">8</figureCitation>
|
||
;
|
||
<tableCitation id="337F03259905FF95FEECFB4574D4FA99" box="[328,452,1267,1294]" captionStart-0="TABLE 1" captionStart-1="TABLE 2" captionStart-2="TABLE 3" captionStart-3="TABLE 4" captionStart-4="TABLE 5" captionStartId-0="2.[151,239,873,899]" captionStartId-1="3.[151,239,743,769]" captionStartId-2="19.[151,239,686,712]" captionStartId-3="20.[151,239,667,693]" captionStartId-4="21.[151,239,620,644]" captionTargetBox-0="[162,2019,914,1334]" captionTargetBox-1="[162,1997,933,1366]" captionTargetBox-2="[162,2023,798,1503]" captionTargetPageId-0="2" captionTargetPageId-1="4" captionTargetPageId-2="19" captionTargetPageId-3="20" captionTargetPageId-4="21" captionText-0="TABLE 1. Material and processing of the basibionts Setaphyes dentatus and S. kielensis with their epibionts collected on the island of Sylt in the North Sea, German Bight." captionText-1="TABLE 2. Number of specimens of Trematosoma husselae sp. nov.on the trunk segments of adult Setaphyeskielensis, light microscopical specimens, collected 1998.Abbreviations: d, dorsal;, exuvia, not counted; v, ventral." captionText-2="TABLE 3. Number of Trematosoma husselae sp. nov. and Cothurnia buetschlii (indicated by C.) on the trunk segments of adult Setaphyes kielensis, SEM specimens, collected 2016. Abbreviations: *, attachment site of a detached suctorian or Cothurnia-like ciliate, not counted; *?, attachment site questionable, not counted; C.:, Cothurnia buetschlii; d, dorsal; fil. epib., filamentous prokaryotic epibionts; Totalsuctorians, total amount of suctorians; v, ventral." captionText-3="TABLE 4. Number of specimens of Trematosoma husselae sp. nov., and Cothurnia buetschlii (indicated by C.) on the trunk segments of adult Setaphyes kielensis, light microscopical specimens, collected 2016. Abbreviations: C.:, Cothurnia buetschlii; d, dorsal; ex, exuvia, not counted; fil. epib., filamentous prokaryotic epibionts; Totalsuctorians, total amount of suctorians; v, ventral." captionText-4="TABLE 5. Number of specimens of Trematosoma husselae sp. nov. on the trunk segments of adult Setaphyes dentatus, light microscopical specimens, collected 2016. Abbreviations: d, dorsal;, exuvia, not counted; v, ventral." httpUri-0="http://table.plazi.org/id/2A8266169901FF91FF33FCDF72D0FC14" httpUri-1="http://table.plazi.org/id/2A8266169900FF90FF33FD517774FCB2" httpUri-2="http://table.plazi.org/id/2A8266169910FF80FF33FD1871C4FC87" httpUri-3="http://table.plazi.org/id/2A8266169917FF87FF33FD2D7735FD6A" httpUri-4="http://table.plazi.org/id/2A8266169916FF86FF33FDDA7015FD3F" pageId="6" pageNumber="445" tableUuid-0="2A8266169901FF91FF33FCDF72D0FC14" tableUuid-1="2A8266169900FF90FF33FD517774FCB2" tableUuid-2="2A8266169910FF80FF33FD1871C4FC87" tableUuid-3="2A8266169917FF87FF33FD2D7735FD6A" tableUuid-4="2A8266169916FF86FF33FDDA7015FD3F">Tables 1–5</tableCitation>
|
||
,
|
||
<tableCitation id="337F03259905FF95FE74FB4574CFFA9A" box="[464,479,1267,1293]" captionStart="TABLE 7" captionStartId="23.[151,244,507,535]" captionTargetBox="[151,2012,513,1718]" captionTargetPageId="24" captionText="TABLE 7. Comparison of characters of species of Trematosoma after original descriptions and additional references. Unique characters marked in bold. Abbreviations: -, information about character not available; +, character present; [], information from additional reference(s).?, character appeared questionable or unclear in our interpretation; SEM, scanning electron microscopy." httpUri="http://table.plazi.org/id/2A8266169914FF84FF33FE4D77C9FDC9" pageId="6" pageNumber="445" tableUuid="2A8266169914FF84FF33FE4D77C9FDC9">7</tableCitation>
|
||
)
|
||
</paragraph>
|
||
<paragraph id="7E42369E9905FF95FF33FA8D740BFAC2" blockId="6.[151,283,1339,1365]" box="[151,283,1339,1365]" pageId="6" pageNumber="445">
|
||
<heading id="250A81F29905FF95FF33FA8D740BFAC2" bold="true" box="[151,283,1339,1365]" fontSize="11" level="1" pageId="6" pageNumber="445" reason="1">
|
||
<emphasis id="4C89EA8C9905FF95FF33FA8D740BFAC2" bold="true" box="[151,283,1339,1365]" pageId="6" pageNumber="445">Synonymy</emphasis>
|
||
</heading>
|
||
</paragraph>
|
||
<paragraph id="7E42369E9905FF95FF33FA35769EFA09" blockId="6.[151,910,1411,1438]" box="[151,910,1411,1438]" pageId="6" pageNumber="445">
|
||
<bibRefCitation id="1A6C4B6F9905FF95FF33FA35742BFA0A" author="Neuhaus, B." box="[151,315,1411,1437]" pageId="6" pageNumber="445" refId="ref17212" refString="Neuhaus, B. (2013) Kinorhyncha (= Echinodera). In: Schmidt-Rhaesa, A. (Ed.), Handbook of Zoology, Gastrotricha, Cycloneuralia and Gnathifera. Vol. 1. Nematomorpha, Priapulida, Kinorhyncha, Loricifera. Walter de Gruyter, Berlin, pp. 181 - 348. https: // doi. org / 10.1515 / 9783110272536.181" type="book" year="2013">Neuhaus 2013</bibRefCitation>
|
||
, p. 331, fig. 5.8.1D, E:
|
||
<taxonomicName id="B9FD4D1D9905FF95FDE5FA3577B1FA0A" box="[577,673,1411,1437]" class="Suctoria" pageId="6" pageNumber="469" rank="class">Suctoria</taxonomicName>
|
||
, possibly
|
||
<taxonomicName id="B9FD4D1D9905FF95FCB5FA35769EFA09" box="[785,910,1411,1438]" class="Kinetofragminophora" family="Acinetidae" genus="Acineta" kingdom="Chromista" order="Suctorida" pageId="6" pageNumber="445" phylum="Ciliophora" rank="species" species="undetermined">
|
||
<emphasis id="4C89EA8C9905FF95FCB5FA357677FA0A" box="[785,871,1411,1437]" italics="true" pageId="6" pageNumber="445">Acineta</emphasis>
|
||
sp.
|
||
</taxonomicName>
|
||
</paragraph>
|
||
</subSubSection>
|
||
<subSubSection id="36E765159905FF95FF33FA597400F99E" box="[151,272,1519,1545]" pageId="6" pageNumber="445" type="diagnosis">
|
||
<paragraph id="7E42369E9905FF95FF33FA597400F99E" blockId="6.[151,272,1519,1545]" box="[151,272,1519,1545]" pageId="6" pageNumber="445">
|
||
<heading id="250A81F29905FF95FF33FA597400F99E" bold="true" box="[151,272,1519,1545]" fontSize="11" level="1" pageId="6" pageNumber="445" reason="1">
|
||
<emphasis id="4C89EA8C9905FF95FF33FA597400F99E" bold="true" box="[151,272,1519,1545]" pageId="6" pageNumber="445">Diagnosis</emphasis>
|
||
</heading>
|
||
</paragraph>
|
||
</subSubSection>
|
||
<subSubSection id="36E765159905FF94FF33F9817691F9EE" lastPageId="7" lastPageNumber="446" pageId="6" pageNumber="445" type="materials_examined">
|
||
<paragraph id="7E42369E9905FF95FF33F9817495F9C6" blockId="6.[151,389,1591,1617]" box="[151,389,1591,1617]" pageId="6" pageNumber="445">
|
||
<heading id="250A81F29905FF95FF33F9817495F9C6" bold="true" box="[151,389,1591,1617]" fontSize="11" level="1" pageId="6" pageNumber="445" reason="1">
|
||
<emphasis id="4C89EA8C9905FF95FF33F9817495F9C6" bold="true" box="[151,389,1591,1617]" pageId="6" pageNumber="445">Material examined</emphasis>
|
||
</heading>
|
||
</paragraph>
|
||
<paragraph id="7E42369E9905FF95FF33F9C97687F8DA" blockId="6.[151,1437,1663,1870]" pageId="6" pageNumber="445">
|
||
Altogether,
|
||
<specimenCount id="68FBFD179905FF95FEBFF9C974D0F90E" box="[283,448,1663,1690]" pageId="6" pageNumber="445" type="generic">705 specimens</specimenCount>
|
||
of
|
||
<taxonomicName id="B9FD4D1D9905FF95FE40F93677F2F90E" authority="Neuhaus & Lu & Yamasaki & González-Casarrubios, 2023" authorityName="Neuhaus & Lu & Yamasaki & González-Casarrubios" authorityYear="2023" box="[484,738,1663,1689]" class="Kinetofragminophora" family="Acinetidae" genus="Trematosoma" higherTaxonomySource="GBIF" kingdom="Chromista" order="Suctorida" pageId="6" pageNumber="445" phylum="Ciliophora" rank="species" species="husselae" status="sp. nov.">
|
||
<emphasis id="4C89EA8C9905FF95FE40F93677F2F90E" box="[484,738,1663,1689]" italics="true" pageId="6" pageNumber="445">Trematosoma husselae</emphasis>
|
||
</taxonomicName>
|
||
<emphasis id="4C89EA8C9905FF95FD4DF9C97652F90E" bold="true" box="[745,834,1663,1689]" pageId="6" pageNumber="445">
|
||
<taxonomicNameLabel id="57BA57F79905FF95FD4DF9C97652F90E" box="[745,834,1663,1689]" pageId="6" pageNumber="445" rank="species">sp. nov.</taxonomicNameLabel>
|
||
</emphasis>
|
||
were studied on its basibionts
|
||
<taxonomicName id="B9FD4D1D9905FF95FB33F9C9707DF90E" baseAuthorityName="Zelinka" baseAuthorityYear="1928" box="[1175,1389,1663,1689]" class="Allomalorhagida" family="Pycnophyidae" genus="Setaphyes" kingdom="Animalia" pageId="6" pageNumber="445" phylum="Kinorhyncha" rank="species" species="kielensis">
|
||
<emphasis id="4C89EA8C9905FF95FB33F9C9707DF90E" box="[1175,1389,1663,1689]" italics="true" pageId="6" pageNumber="445">Setaphyes kielensis</emphasis>
|
||
</taxonomicName>
|
||
and
|
||
<taxonomicName id="B9FD4D1D9905FF95FF33F9157401F92A" baseAuthorityName="Reinhard" baseAuthorityYear="1881" box="[151,273,1699,1725]" class="Allomalorhagida" family="Pycnophyidae" genus="Setaphyes" kingdom="Animalia" pageId="6" pageNumber="445" phylum="Kinorhyncha" rank="species" species="dentatus">
|
||
<emphasis id="4C89EA8C9905FF95FF33F9157401F92A" box="[151,273,1699,1725]" italics="true" pageId="6" pageNumber="445">S. dentatus</emphasis>
|
||
</taxonomicName>
|
||
(Kinorhyncha), of these
|
||
<specimenCount id="68FBFD179905FF95FD83F91577DCF92A" box="[551,716,1699,1725]" pageId="6" pageNumber="445" type="generic">308 specimens</specimenCount>
|
||
by light microscopy and
|
||
<specimenCount id="68FBFD179905FF95FC41F915719AF92A" box="[997,1162,1699,1725]" pageId="6" pageNumber="445" type="generic">397 specimens</specimenCount>
|
||
by SEM. The basibionts were deposited at the Museum für Naturkunde
|
||
<collectingRegion id="BC39F87C9905FF95FD06F97177F8F976" box="[674,744,1735,1761]" country="Germany" name="Berlin" pageId="6" pageNumber="445">Berlin</collectingRegion>
|
||
in the collection "Vermes" under the catalogue numbers
|
||
<collectionCode id="18ECAE5B9905FF95FAFBF97E708CF975" box="[1375,1436,1736,1762]" country="Germany" httpUri="http://grbio.org/cool/8syf-d21i" name="Museum für Naturkunde Berlin (Zoological Collections)" pageId="6" pageNumber="445" type="Museum">ZMB</collectionCode>
|
||
12294–12313, 12359–12418, and 12454–12514. The
|
||
<typeStatus id="A146883C9905FF95FD5FF95D764DF892" box="[763,861,1771,1797]" pageId="6" pageNumber="445">holotype</typeStatus>
|
||
represents the specimen with a swarmer ventrally on segment 1 of the basibiont
|
||
<collectionCode id="18ECAE5B9905FF95FE5CF8A67725F8BD" box="[504,565,1808,1834]" country="Germany" httpUri="http://grbio.org/cool/8syf-d21i" name="Museum für Naturkunde Berlin (Zoological Collections)" pageId="6" pageNumber="445" type="Museum">ZMB</collectionCode>
|
||
12499. All light microscopical epibionts on the basibiont
|
||
<taxonomicName id="B9FD4D1D9905FF95FB73F8B9708DF8BD" authority="ZMB" authorityName="ZMB" box="[1239,1437,1807,1834]" class="Allomalorhagida" family="Pycnophyidae" genus="Setaphyes" kingdom="Animalia" pageId="6" pageNumber="445" phylum="Kinorhyncha" rank="species" species="kielensis">
|
||
<emphasis id="4C89EA8C9905FF95FB73F8B97046F8BE" box="[1239,1366,1807,1833]" italics="true" pageId="6" pageNumber="445">S. kielensis</emphasis>
|
||
ZMB
|
||
</taxonomicName>
|
||
12479–12505 represent
|
||
<typeStatus id="A146883C9905FF95FE02F8827705F8D9" box="[422,533,1844,1870]" pageId="6" pageNumber="445">paratypes</typeStatus>
|
||
. For collecting details see
|
||
<tableCitation id="337F03259905FF95FC99F8857680F8DA" box="[829,912,1843,1869]" captionStart="TABLE 1" captionStartId="2.[151,239,873,899]" captionTargetPageId="2" captionText="TABLE 1. Material and processing of the basibionts Setaphyes dentatus and S. kielensis with their epibionts collected on the island of Sylt in the North Sea, German Bight." httpUri="http://table.plazi.org/id/2A8266169901FF91FF33FCDF72D0FC14" pageId="6" pageNumber="445" tableUuid="2A8266169901FF91FF33FCDF72D0FC14">Table 1</tableCitation>
|
||
.
|
||
</paragraph>
|
||
<caption id="2A8266169904FF94FF33FC277454FBAD" ID-DOI="http://doi.org/10.5281/zenodo.8345483" ID-Zenodo-Dep="8345483" httpUri="https://zenodo.org/record/8345483/files/figure.png" pageId="7" pageNumber="446" startId="7.[151,250,913,937]" targetBox="[253,1340,184,889]" targetPageId="7" targetType="figure">
|
||
<paragraph id="7E42369E9904FF94FF33FC277454FBAD" blockId="7.[151,1437,913,1082]" pageId="7" pageNumber="446">
|
||
<emphasis id="4C89EA8C9904FF94FF33FC277407FC3E" bold="true" box="[151,279,913,937]" pageId="7" pageNumber="446">FIGURE 2.</emphasis>
|
||
<taxonomicName id="B9FD4D1D9904FF94FE85FC24771BFC3E" authority="Neuhaus & Lu & Yamasaki & González-Casarrubios, 2023" authorityName="Neuhaus & Lu & Yamasaki & González-Casarrubios" authorityYear="2023" box="[289,523,913,937]" class="Kinetofragminophora" family="Acinetidae" genus="Trematosoma" higherTaxonomySource="GBIF" kingdom="Chromista" order="Suctorida" pageId="7" pageNumber="446" phylum="Ciliophora" rank="species" species="husselae" status="sp. nov.">
|
||
<emphasis id="4C89EA8C9904FF94FE85FC24771BFC3E" box="[289,523,913,937]" italics="true" pageId="7" pageNumber="446">Trematosoma husselae</emphasis>
|
||
</taxonomicName>
|
||
<emphasis id="4C89EA8C9904FF94FDB1FC277779FC3E" bold="true" box="[533,617,913,937]" pageId="7" pageNumber="446">
|
||
<taxonomicNameLabel id="57BA57F79904FF94FDB1FC277779FC3E" box="[533,617,913,937]" pageId="7" pageNumber="446" rank="species">sp. nov.</taxonomicNameLabel>
|
||
</emphasis>
|
||
on
|
||
<taxonomicName id="B9FD4D1D9904FF94FD33FC27764EFC3E" box="[663,862,913,937]" class="Allomalorhagida" family="Pycnophyidae" genus="Setaphyes" kingdom="Animalia" pageId="7" pageNumber="446" phylum="Kinorhyncha" rank="species" species="kielensis">
|
||
<emphasis id="4C89EA8C9904FF94FD33FC27764EFC3E" box="[663,862,913,937]" italics="true" pageId="7" pageNumber="446">Setaphyes kielensis</emphasis>
|
||
</taxonomicName>
|
||
.
|
||
<emphasis id="4C89EA8C9904FF94FCC9FC27766FFC3E" bold="true" box="[877,895,913,937]" pageId="7" pageNumber="446">A</emphasis>
|
||
. Drawing of the holotype with internal developing swarmer ventrally on segment 1 of basibiont (ZMB 12499).
|
||
<emphasis id="4C89EA8C9904FF94FD5CFC037619FC5A" bold="true" box="[760,777,949,973]" pageId="7" pageNumber="446">B</emphasis>
|
||
. Paratype in side view ventrally on segment 3 of basibiont (ZMB 12489). Arrow marks apical cleft within lorica. Abbreviations: ca, basal cavity in lorica; lo, lorica; ma, macronucleus; mi, micronucleus; st, stalk with internal striation (information taken from different specimen); sw, swarmer; te, tentacle; zo, zooid. Scale bar 30 µm.
|
||
</paragraph>
|
||
</caption>
|
||
<paragraph id="7E42369E9904FF94FF33FB3A7427FB32" blockId="7.[151,311,1163,1190]" box="[151,311,1163,1190]" pageId="7" pageNumber="446">
|
||
<heading id="250A81F29904FF94FF33FB3A7427FB32" bold="true" box="[151,311,1163,1190]" fontSize="11" level="1" pageId="7" pageNumber="446" reason="1">
|
||
<emphasis id="4C89EA8C9904FF94FF33FB3A7427FB32" bold="true" box="[151,311,1163,1190]" pageId="7" pageNumber="446">
|
||
<typeStatus id="A146883C9904FF94FF33FB3A75C4FB31" box="[151,212,1164,1190]" pageId="7" pageNumber="446">Type</typeStatus>
|
||
locality
|
||
</emphasis>
|
||
</heading>
|
||
</paragraph>
|
||
<paragraph id="7E42369E9904FF94FF33FB657455FA85" blockId="7.[151,1437,1235,1298]" pageId="7" pageNumber="446">
|
||
Intertidal mud at Kampen, east coast of the island of Sylt, German Bight, North Sea,
|
||
<geoCoordinate id="1BC950599904FF94FBCCFB6571E3FB79" box="[1128,1267,1235,1262]" degrees="54" direction="north" minutes="57" orientation="latitude" pageId="7" pageNumber="446" precision="15" seconds="15" value="54.954166">54°57’15”N</geoCoordinate>
|
||
,
|
||
<geoCoordinate id="1BC950599904FF94FAA5FB627087FB79" box="[1281,1431,1236,1262]" degrees="008" direction="east" minutes="21" orientation="longitude" pageId="7" pageNumber="446" precision="15" seconds="34" value="8.359445">008°21’34”E</geoCoordinate>
|
||
, precision
|
||
<specimenCount id="68FBFD179904FF94FEA1FB417455FA85" box="[261,325,1271,1298]" pageId="7" pageNumber="446" type="generic">
|
||
<quantity id="B9059B7B9904FF94FEA1FB417451FA85" box="[261,321,1271,1298]" metricMagnitude="1" metricUnit="m" metricValue="5.0" pageId="7" pageNumber="446" unit="m" value="50.0">50 m</quantity>
|
||
.
|
||
</specimenCount>
|
||
</paragraph>
|
||
<paragraph id="7E42369E9904FF94FF33FAD27440FAEA" blockId="7.[151,336,1379,1406]" box="[151,336,1379,1406]" pageId="7" pageNumber="446">
|
||
<heading id="250A81F29904FF94FF33FAD27440FAEA" bold="true" box="[151,336,1379,1406]" fontSize="11" level="1" pageId="7" pageNumber="446" reason="1">
|
||
<emphasis id="4C89EA8C9904FF94FF33FAD27440FAEA" bold="true" box="[151,336,1379,1406]" pageId="7" pageNumber="446">
|
||
<typeStatus id="A146883C9904FF94FF33FAD275C4FAE9" box="[151,212,1380,1406]" pageId="7" pageNumber="446">Type</typeStatus>
|
||
basibiont
|
||
</emphasis>
|
||
</heading>
|
||
</paragraph>
|
||
<paragraph id="7E42369E9904FF94FF33FA1D7661FA52" blockId="7.[151,881,1451,1478]" box="[151,881,1451,1478]" pageId="7" pageNumber="446">
|
||
<emphasis id="4C89EA8C9904FF94FF33FA1D747EFA52" box="[151,366,1451,1477]" italics="true" pageId="7" pageNumber="446">Setaphyes kielensis</emphasis>
|
||
(
|
||
<bibRefCitation id="1A6C4B6F9904FF94FED9FA1D770EFA51" author="Zelinka, K." box="[381,542,1451,1478]" pageId="7" pageNumber="446" refId="ref18386" refString="Zelinka, K. (1928) Monographie der Echinodera. Verlag von Wilhelm Engelmann, Leipzig, 396 pp., 27 pls." type="book" year="1928">Zelinka, 1928</bibRefCitation>
|
||
), Kinorhyncha,
|
||
<taxonomicName id="B9FD4D1D9904FF94FD76FA1D7661FA52" authorityName="Zelinka" authorityYear="1896" box="[722,881,1451,1477]" class="Allomalorhagida" family="Pycnophyidae" kingdom="Animalia" pageId="7" pageNumber="446" phylum="Kinorhyncha" rank="family">Pycnophyidae</taxonomicName>
|
||
</paragraph>
|
||
<paragraph id="7E42369E9904FF94FF33F9A17488F9A6" blockId="7.[151,408,1559,1585]" box="[151,408,1559,1585]" pageId="7" pageNumber="446">
|
||
<heading id="250A81F29904FF94FF33F9A17488F9A6" bold="true" box="[151,408,1559,1585]" fontSize="11" level="1" pageId="7" pageNumber="446" reason="1">
|
||
<emphasis id="4C89EA8C9904FF94FF33F9A17488F9A6" bold="true" box="[151,408,1559,1585]" pageId="7" pageNumber="446">Additional basibiont</emphasis>
|
||
</heading>
|
||
</paragraph>
|
||
<paragraph id="7E42369E9904FF94FF33F9E97691F9EE" blockId="7.[151,897,1631,1658]" box="[151,897,1631,1658]" pageId="7" pageNumber="446">
|
||
<taxonomicName id="B9FD4D1D9904FF94FF33F9E97725F9ED" authority="(Reinhard, 1881)" baseAuthorityName="Reinhard" baseAuthorityYear="1881" box="[151,565,1631,1658]" class="Allomalorhagida" family="Pycnophyidae" genus="Setaphyes" kingdom="Animalia" pageId="7" pageNumber="446" phylum="Kinorhyncha" rank="species" species="dentatus">
|
||
<emphasis id="4C89EA8C9904FF94FF33F9E9747DF9EE" box="[151,365,1631,1657]" italics="true" pageId="7" pageNumber="446">Setaphyes dentatus</emphasis>
|
||
(
|
||
<bibRefCitation id="1A6C4B6F9904FF94FED8F9E9773DF9ED" author="Reinhard, W." box="[380,557,1631,1658]" pageId="7" pageNumber="446" pagination="588 - 592" refId="ref17713" refString="Reinhard, W. (1881) Uber Echinoderes und Desmoscolex der Umgebung von Odessa. Zoologischer Anzeiger, 4 (97), 588 - 592." type="journal article" year="1881">Reinhard, 1881</bibRefCitation>
|
||
)
|
||
</taxonomicName>
|
||
, Kinorhyncha,
|
||
<taxonomicName id="B9FD4D1D9904FF94FD46F9E97691F9EE" authorityName="Zelinka" authorityYear="1896" box="[738,897,1631,1657]" class="Allomalorhagida" family="Pycnophyidae" kingdom="Animalia" pageId="7" pageNumber="446" phylum="Kinorhyncha" rank="family">Pycnophyidae</taxonomicName>
|
||
</paragraph>
|
||
</subSubSection>
|
||
<subSubSection id="36E765159904FF94FF33F97A7030F8E1" pageId="7" pageNumber="446" type="etymology">
|
||
<paragraph id="7E42369E9904FF94FF33F97A740EF971" blockId="7.[151,286,1740,1766]" box="[151,286,1740,1766]" pageId="7" pageNumber="446">
|
||
<heading id="250A81F29904FF94FF33F97A740EF971" bold="true" box="[151,286,1740,1766]" fontSize="11" level="1" pageId="7" pageNumber="446" reason="1">
|
||
<emphasis id="4C89EA8C9904FF94FF33F97A740EF971" bold="true" box="[151,286,1740,1766]" pageId="7" pageNumber="446">Etymology</emphasis>
|
||
</heading>
|
||
</paragraph>
|
||
<paragraph id="7E42369E9904FF94FF33F8A57030F8E1" blockId="7.[151,1436,1811,1910]" pageId="7" pageNumber="446">
|
||
The species is named after Birgit Hussel, previously technician at the Litoralstation Sylt, in recognition of her help to B.N. and H.Y. with collecting the specimens and her engagement over many years in sending meiofauna samples from Sylt to the first author for his public outreach activities in
|
||
<collectingCountry id="06EA760E9904FF94FCFAF8EA76D6F8E1" box="[862,966,1884,1910]" name="Germany" pageId="7" pageNumber="446">Germany</collectingCountry>
|
||
and
|
||
<collectingCountry id="06EA760E9904FF94FC59F8ED7146F8E2" box="[1021,1110,1883,1909]" name="Sweden" pageId="7" pageNumber="446">Sweden</collectingCountry>
|
||
during the 2000s.
|
||
</paragraph>
|
||
</subSubSection>
|
||
<caption id="2A826616990BFF9BFF33F96076ABF835" ID-DOI="http://doi.org/10.5281/zenodo.8345485" ID-Zenodo-Dep="8345485" httpUri="https://zenodo.org/record/8345485/files/figure.png" pageId="8" pageNumber="447" startId="8.[151,250,1750,1774]" targetBox="[151,1436,181,1726]" targetPageId="8" targetType="figure">
|
||
<paragraph id="7E42369E990BFF9BFF33F96076ABF835" blockId="8.[151,1437,1750,1954]" pageId="8" pageNumber="447">
|
||
<emphasis id="4C89EA8C990BFF9BFF33F9607406F979" bold="true" box="[151,278,1750,1774]" pageId="8" pageNumber="447">FIGURE 3.</emphasis>
|
||
<taxonomicName id="B9FD4D1D990BFF9BFEBBF96074F5F979" baseAuthorityName="Zelinka" baseAuthorityYear="1928" box="[287,485,1750,1774]" class="Allomalorhagida" family="Pycnophyidae" genus="Setaphyes" kingdom="Animalia" pageId="8" pageNumber="447" phylum="Kinorhyncha" rank="species" species="kielensis">
|
||
<emphasis id="4C89EA8C990BFF9BFEBBF96074F5F979" box="[287,485,1750,1774]" italics="true" pageId="8" pageNumber="447">Setaphyes kielensis</emphasis>
|
||
</taxonomicName>
|
||
(
|
||
<emphasis id="4C89EA8C990BFF9BFE53F9607738F979" bold="true" box="[503,552,1750,1774]" pageId="8" pageNumber="447">A–C</emphasis>
|
||
, ZMB 12475;
|
||
<emphasis id="4C89EA8C990BFF9BFD63F96077C9F979" bold="true" box="[711,729,1750,1774]" pageId="8" pageNumber="447">D</emphasis>
|
||
, ZMB 12464;
|
||
<emphasis id="4C89EA8C990BFF9BFCDDF960769AF979" bold="true" box="[889,906,1750,1774]" pageId="8" pageNumber="447">E</emphasis>
|
||
,
|
||
<emphasis id="4C89EA8C990BFF9BFC3DF96076B8F979" bold="true" box="[921,936,1750,1774]" pageId="8" pageNumber="447">F</emphasis>
|
||
, ZMB 12465;
|
||
<emphasis id="4C89EA8C990BFF9BFBECF960714CF979" bold="true" box="[1096,1116,1750,1774]" pageId="8" pageNumber="447">G</emphasis>
|
||
, ZMB 12462) with specimens of
|
||
<taxonomicName id="B9FD4D1D990BFF9BFF11F94D748EF885" authority="Neuhaus & Lu & Yamasaki & González-Casarrubios, 2023" authorityName="Neuhaus & Lu & Yamasaki & González-Casarrubios" authorityYear="2023" box="[181,414,1786,1810]" class="Kinetofragminophora" family="Acinetidae" genus="Trematosoma" higherTaxonomySource="GBIF" kingdom="Chromista" order="Suctorida" pageId="8" pageNumber="447" phylum="Ciliophora" rank="species" species="husselae" status="sp. nov.">
|
||
<emphasis id="4C89EA8C990BFF9BFF11F94D748EF885" box="[181,414,1786,1810]" italics="true" pageId="8" pageNumber="447">Trematosoma husselae</emphasis>
|
||
</taxonomicName>
|
||
<emphasis id="4C89EA8C990BFF9BFE0CF94C74EBF885" bold="true" box="[424,507,1786,1810]" pageId="8" pageNumber="447">
|
||
<taxonomicNameLabel id="57BA57F7990BFF9BFE0CF94C74EBF885" box="[424,507,1786,1810]" pageId="8" pageNumber="447" rank="species">sp. nov.</taxonomicNameLabel>
|
||
</emphasis>
|
||
, SEM, ventral view.
|
||
<emphasis id="4C89EA8C990BFF9BFD78F94C7604F885" bold="true" box="[732,788,1786,1810]" pageId="8" pageNumber="447">A–C.</emphasis>
|
||
Specimen with 43 epibionts, segments 1–4 (
|
||
<emphasis id="4C89EA8C990BFF9BFB49F94C71EFF885" bold="true" box="[1261,1279,1786,1810]" pageId="8" pageNumber="447">A</emphasis>
|
||
), 5–7 (
|
||
<emphasis id="4C89EA8C990BFF9BFAEBF94C7070F885" bold="true" box="[1359,1376,1786,1810]" pageId="8" pageNumber="447">B</emphasis>
|
||
), and 8–11 (
|
||
<emphasis id="4C89EA8C990BFF9BFF7CF8A875FAF8A1" bold="true" box="[216,234,1822,1846]" pageId="8" pageNumber="447">C</emphasis>
|
||
).
|
||
<emphasis id="4C89EA8C990BFF9BFEA4F8A87409F8A1" bold="true" box="[256,281,1822,1846]" pageId="8" pageNumber="447">D.</emphasis>
|
||
Segment 8, epibiont surface.
|
||
<emphasis id="4C89EA8C990BFF9BFDEDF8A87770F8A1" bold="true" box="[585,608,1822,1846]" pageId="8" pageNumber="447">E.</emphasis>
|
||
Segment 6, epibiont surface covered with detritus.
|
||
<emphasis id="4C89EA8C990BFF9BFBC9F8A87190F8A1" bold="true" box="[1133,1152,1822,1846]" pageId="8" pageNumber="447">F.</emphasis>
|
||
Segment 8, frontal view on small spherical specimen of
|
||
<taxonomicName id="B9FD4D1D990BFF9BFE1EF8F577B1F8CD" authority="Neuhaus & Lu & Yamasaki & González-Casarrubios, 2023" authorityName="Neuhaus & Lu & Yamasaki & González-Casarrubios" authorityYear="2023" box="[442,673,1858,1882]" class="Kinetofragminophora" family="Acinetidae" genus="Trematosoma" higherTaxonomySource="GBIF" kingdom="Chromista" order="Suctorida" pageId="8" pageNumber="447" phylum="Ciliophora" rank="species" species="husselae" status="sp. nov.">
|
||
<emphasis id="4C89EA8C990BFF9BFE1EF8F577B1F8CD" box="[442,673,1858,1882]" italics="true" pageId="8" pageNumber="447">Trematosoma husselae</emphasis>
|
||
</taxonomicName>
|
||
<emphasis id="4C89EA8C990BFF9BFD0CF8F4760BF8CD" bold="true" box="[680,795,1858,1882]" pageId="8" pageNumber="447">
|
||
<taxonomicNameLabel id="57BA57F7990BFF9BFD0CF8F477EAF8CD" box="[680,762,1858,1882]" pageId="8" pageNumber="447" rank="species">sp. nov.</taxonomicNameLabel>
|
||
G.
|
||
</emphasis>
|
||
Segment 10, arrow marks specimen with withdrawn tentacles. Abbreviations: gco, gland cell outlet; lts, lateral terminal spine; s, segment, followed by segment number; se, seta; su, suctorian epibiont; te, tentacle. Scale bar in
|
||
<emphasis id="4C89EA8C990BFF9BFE54F83C7731F835" bold="true" box="[496,545,1930,1954]" pageId="8" pageNumber="447">A–C</emphasis>
|
||
40 µm, in
|
||
<emphasis id="4C89EA8C990BFF9BFD34F83C77AEF835" bold="true" box="[656,702,1930,1954]" pageId="8" pageNumber="447">D–F</emphasis>
|
||
10 µm, and in
|
||
<emphasis id="4C89EA8C990BFF9BFCFCF83C767CF835" bold="true" box="[856,876,1930,1954]" pageId="8" pageNumber="447">G</emphasis>
|
||
20 µm.
|
||
</paragraph>
|
||
</caption>
|
||
<subSubSection id="36E76515990AFF9AFF33FF2174CFFED6" pageId="9" pageNumber="448" type="diagnosis">
|
||
<paragraph id="7E42369E990AFF9AFF33FF217400FF26" blockId="9.[151,272,151,177]" box="[151,272,151,177]" pageId="9" pageNumber="448">
|
||
<heading id="250A81F2990AFF9AFF33FF217400FF26" bold="true" box="[151,272,151,177]" fontSize="11" level="1" pageId="9" pageNumber="448" reason="1">
|
||
<emphasis id="4C89EA8C990AFF9AFF33FF217400FF26" bold="true" box="[151,272,151,177]" pageId="9" pageNumber="448">Diagnosis</emphasis>
|
||
</heading>
|
||
</paragraph>
|
||
<paragraph id="7E42369E990AFF9AFF33FF6974CFFED6" blockId="9.[151,1437,223,322]" pageId="9" pageNumber="448">Acinetid with calyciform, dorso-ventrally compressed lorica of about 35 µm x 30 µm size, short stalk with internal longitudinal striae, one left and one right row of tentacles ca. 5 µm long, tentacles separated by small gap, epibiontic on pycnophyid Kinorhyncha.</paragraph>
|
||
</subSubSection>
|
||
<subSubSection id="36E76515990AFF9AFF33FE257164F891" pageId="9" pageNumber="448" type="description">
|
||
<paragraph id="7E42369E990AFF9AFF33FE257438FE3A" blockId="9.[151,296,403,429]" box="[151,296,403,429]" pageId="9" pageNumber="448">
|
||
<heading id="250A81F2990AFF9AFF33FE257438FE3A" bold="true" box="[151,296,403,429]" fontSize="11" level="1" pageId="9" pageNumber="448" reason="1">
|
||
<emphasis id="4C89EA8C990AFF9AFF33FE257438FE3A" bold="true" box="[151,296,403,429]" pageId="9" pageNumber="448">Description</emphasis>
|
||
</heading>
|
||
</paragraph>
|
||
<paragraph id="7E42369E990AFF9AFF33FE6D709DFC5E" blockId="9.[151,1437,475,1798]" pageId="9" pageNumber="448">
|
||
Specimens of
|
||
<taxonomicName id="B9FD4D1D990AFF9AFEE4FE6A7754FE62" authority="Neuhaus & Lu & Yamasaki & González-Casarrubios, 2023" authorityName="Neuhaus & Lu & Yamasaki & González-Casarrubios" authorityYear="2023" box="[320,580,475,501]" class="Kinetofragminophora" family="Acinetidae" genus="Trematosoma" higherTaxonomySource="GBIF" kingdom="Chromista" order="Suctorida" pageId="9" pageNumber="448" phylum="Ciliophora" rank="species" species="husselae" status="sp. nov.">
|
||
<emphasis id="4C89EA8C990AFF9AFEE4FE6A7754FE62" box="[320,580,475,501]" italics="true" pageId="9" pageNumber="448">Trematosoma husselae</emphasis>
|
||
</taxonomicName>
|
||
<emphasis id="4C89EA8C990AFF9AFDF4FE6D77BFFE62" bold="true" box="[592,687,475,501]" pageId="9" pageNumber="448">
|
||
<taxonomicNameLabel id="57BA57F7990AFF9AFDF4FE6D77BFFE62" box="[592,687,475,501]" pageId="9" pageNumber="448" rank="species">sp. nov.</taxonomicNameLabel>
|
||
</emphasis>
|
||
sat on a short stalk and revealed a calyciform, dorso-ventrally compressed lorica (lorica
|
||
<typeStatus id="A146883C990AFF9AFE1CFE49770EFD8E" box="[440,542,511,537]" pageId="9" pageNumber="448">holotype</typeStatus>
|
||
: 37 µm length x 31µm width; range 30–47 µm x 26–34 µm, thickness 9–16 µm) with the zooid (
|
||
<figureCitation id="E6C62A1B990AFF9AFEE1FD95749DFDAA" box="[325,397,547,573]" captionStart="FIGURE 2" captionStartId="7.[151,250,913,937]" captionTargetBox="[253,1340,184,889]" captionTargetId="figure-17@7.[248,1340,181,889]" captionTargetPageId="7" captionText="FIGURE 2. Trematosoma husselae sp. nov. on Setaphyes kielensis. A. Drawing of the holotype with internal developing swarmer ventrally on segment 1 of basibiont (ZMB 12499). B. Paratype in side view ventrally on segment 3 of basibiont (ZMB 12489). Arrow marks apical cleft within lorica. Abbreviations: ca, basal cavity in lorica; lo, lorica; ma, macronucleus; mi, micronucleus; st, stalk with internal striation (information taken from different specimen); sw, swarmer; te, tentacle; zo, zooid. Scale bar 30 µm." figureDoi="http://doi.org/10.5281/zenodo.8345483" httpUri="https://zenodo.org/record/8345483/files/figure.png" pageId="9" pageNumber="448">Figs 2</figureCitation>
|
||
,
|
||
<figureCitation id="E6C62A1B990AFF9AFE3DFD9574B8FDAA" box="[409,424,547,573]" captionStart="FIGURE 3" captionStartId="8.[151,250,1750,1774]" captionTargetBox="[151,1436,181,1726]" captionTargetId="figure-24@8.[151,1436,181,1726]" captionTargetPageId="8" captionText="FIGURE 3. Setaphyes kielensis (A–C, ZMB 12475; D, ZMB 12464; E, F, ZMB 12465; G, ZMB 12462) with specimens of Trematosoma husselae sp. nov., SEM, ventral view. A–C. Specimen with 43 epibionts, segments 1–4 (A), 5–7 (B), and 8–11 (C). D. Segment 8, epibiont surface. E. Segment 6, epibiont surface covered with detritus. F. Segment 8, frontal view on small spherical specimen of Trematosoma husselae sp. nov. G. Segment 10, arrow marks specimen with withdrawn tentacles. Abbreviations: gco, gland cell outlet; lts, lateral terminal spine; s, segment, followed by segment number; se, seta; su, suctorian epibiont; te, tentacle. Scale bar in A–C 40 µm, in D–F 10 µm, and in G 20 µm." figureDoi="http://doi.org/10.5281/zenodo.8345485" httpUri="https://zenodo.org/record/8345485/files/figure.png" pageId="9" pageNumber="448">3</figureCitation>
|
||
,
|
||
<figureCitation id="E6C62A1B990AFF9AFE11FD9574E3FDAA" box="[437,499,547,573]" captionStart="FIGURE 4" captionStartId="10.[151,250,1749,1773]" captionTargetBox="[152,1436,181,1725]" captionTargetId="figure-24@10.[152,1436,181,1725]" captionTargetPageId="10" captionText="FIGURE 4. Setaphyes kielensis (A, ZMB 12469; B, ZMB 12489; C, ZMB 12486; D, E, ZMB 12492; F, ZMB 12488) with specimens of Trematosoma husselae sp. nov., SEM (A), and DIC (B–F). A. Segment 1, empty shells of epibionts, ventral view. B. Segments 1–11, optical section. C. Epibionts with capitated tentacles arranged in left and right row. Extended focus image of three images. D. Epibiont with developing swarmer. Arrowheads mark membrane of swarmer. E. Epibiont with swarmer recently freed from its parent. F. Segments 4–6, ventral view. Note thickened lorica in basal two thirds of epibionts.Arrow marks longitudinal striation of stalk of epibiont. Abbreviations: ca, basal cavity in lorica; co, specimen of Cothurnia buetschlii; lo, lorica; lts, lateral terminal spine; ma, macronucleus; mi, micronucleus; s, segment, followed by segment number; su, suctorian epibiont; sw, swarmer; te, tentacle. Scale bar in A, D, E 20 µm, in B 100 µm, in C 30 µm, and in F 50 µm." figureDoi="http://doi.org/10.5281/zenodo.8345487" httpUri="https://zenodo.org/record/8345487/files/figure.png" pageId="9" pageNumber="448">4B–F</figureCitation>
|
||
,
|
||
<figureCitation id="E6C62A1B990AFF9AFE5AFD957754FDA9" box="[510,580,547,574]" captionStart="FIGURE 5" captionStartId="12.[151,250,186,210]" captionTargetId="figure-17@11.[151,1436,181,1725]" captionTargetPageId="11" captionText="FIGURE 5. Setaphyes kielensis (A, ZMB 12489; B, ZMB 12500; C, ZMB 12499; D, ZMB 12487; J, ZMB 12486; L, ZMB 12397; M, ZMB 12369) and S. dentatus (E–G, K, ZMB 12503; F, H, I, ZMB 12494) with specimens of Trematosoma husselae sp. nov. (A–G, J–M), Cothurnia buetschlii (J), and a case of fungus or developmental artefact (H, I, ZMB 12494), DIC. A. Segments 1–4, lateral and dorso-ventral view on the calyciform, dorso-ventrally compressed lorica with the zooid, ventrolateral view on basibiont. B. Epibiont with swarmer, stalk, and lorica in optical section. C. Epibiont with two macronuclei not fusing at any focal level. D, E. Young, small, spherical epibionts with few tentacles, not all tentacles in focus. F. Young specimen on cuticle of basibiont with striation pattern on surface (arrows). G. Epibiont with tentacles in empty shell of suctorian epibiont. H, I. Segment 10, dorsally (H) and optical section (I), subcuticular fungus or internal sclerotisation artefact of the cuticle (arrows). J. Assemblage of epibionts on segment 10 of basibiont with C. buetschlii (contracted specimen left, extended specimen right), T. husselae sp. nov., and two kinds of filamentous bacteria. Arrows mark endostyle. K. Suctorian on segment 7 of S. dentatus. L, M. Specimens collected 1998, showing cellular substructures (L) and withdrawn zooid with minimum amount of cellular substructures (M). Extended focus images of six (D), eight (H, L), and three (K) images. Arrowheads mark stalks in B, D, E, G, and J. Abbreviations: ba, bacteria; ca, basal cavity in lorica; co, C. buetschlii; en, endostyle; lo, lorica; ma, macronucleus; s, segment, followed by segment number; sc, spherical cavity; sp, sperm; st, stalk; su, suctorian epibiont; sw, swarmer; te, tentacle; tu, tube on segment 2. Scale bar in A 50 µm, in B 20 µm, valid for B, G–J and M, in C 20 µm, valid for C, K, and L, and in D–F 10 µm." figureDoi="http://doi.org/10.5281/zenodo.8349223" httpUri="https://zenodo.org/record/8349223/files/figure.png" pageId="9" pageNumber="448">5A–C</figureCitation>
|
||
,
|
||
<figureCitation id="E6C62A1B990AFF9AFDEBFD92774FFDA9" box="[591,607,548,574]" captionStart="FIGURE 7" captionStartId="14.[151,250,1739,1763]" captionTargetBox="[151,1436,181,1715]" captionTargetId="figure-24@14.[151,1436,181,1715]" captionTargetPageId="14" captionText="FIGURE 7. Setaphyes kielensis (ZMB 12468) with epibionts, SEM. A. Segments 1–11, ventral-right. B. Segments 4–8, ventral view. Arrows mark laterally recessed anterior margin of lorica of Cothurnia buetschlii. Abbreviations: co, Cothurnia buetschlii; fp, filamentous prokaryotes; lts, lateral terminal spine; s, segment, followed by segment number; se, seta; su, suctorian epibiont; tu, tube. Scale bar in A 100 µm and in B 20 µm." figureDoi="http://doi.org/10.5281/zenodo.8345491" httpUri="https://zenodo.org/record/8345491/files/figure.png" pageId="9" pageNumber="448">7</figureCitation>
|
||
,
|
||
<figureCitation id="E6C62A1B990AFF9AFDCEFD957769FDAA" box="[618,633,547,573]" captionStart="FIGURE 8" captionStartId="15.[151,250,1745,1769]" captionTargetBox="[151,1436,182,1721]" captionTargetId="figure-17@15.[151,1436,181,1721]" captionTargetPageId="15" captionText="FIGURE 8. Setaphyes kielensis (A, ZMB 12468; B, ZMB 12474) with epibionts, SEM. A. Segment 6, left side. Arrow marks laterally recessed anterior margin of lorica of Cothurnia buetschlii. B. Segments 8–10, dorsal view.Abbreviations: co, Cothurnia buetschlii; fp, filamentous prokaryotes; s, segment, followed by segment number; su, suctorian epibiont; te, tentacles. Scale bar in A 10 µm and in B 20 µm." figureDoi="http://doi.org/10.5281/zenodo.8345493" httpUri="https://zenodo.org/record/8345493/files/figure.png" pageId="9" pageNumber="448">8</figureCitation>
|
||
,
|
||
<figureCitation id="E6C62A1B990AFF9AFD22FD9577A9FDAA" box="[646,697,547,573]" captionStart="FIGURE 10" captionStartId="18.[151,250,1714,1738]" captionTargetBox="[151,1436,181,1690]" captionTargetId="figure-24@18.[151,1436,181,1690]" captionTargetPageId="18" captionText="FIGURE 10. Setaphyes kielensis (A–C, ZMB 12458; D, ZMB 12488) with cuticular marks and filamentous prokaryotes, SEM (A–C) and DIC (D). A. Cuticular marks (arrows) of detached ciliate specimens, laterodorsal view. B. Segment 1 with large mucous? attachment sites to basibiont (arrows), frontal view. C. Segments 7–11, ventral view. D. Segments 8–10, ventral view. Abbreviations: co, Cothurnia buetschlii; fp, filamentous prokaryotes; lts, lateral terminal spine; pl, placid; s, segment, followed by segment number; su, suctorian epibiont. Scale bar in A–C 20 µm and in D 50 µm." figureDoi="http://doi.org/10.5281/zenodo.8345501" httpUri="https://zenodo.org/record/8345501/files/figure.png" pageId="9" pageNumber="448">10C</figureCitation>
|
||
). The zooid usually filled the lorica except for a small basal cavity (
|
||
<figureCitation id="E6C62A1B990AFF9AFF3BFDF175F8FDF6" box="[159,232,583,609]" captionStart="FIGURE 2" captionStartId="7.[151,250,913,937]" captionTargetBox="[253,1340,184,889]" captionTargetId="figure-17@7.[248,1340,181,889]" captionTargetPageId="7" captionText="FIGURE 2. Trematosoma husselae sp. nov. on Setaphyes kielensis. A. Drawing of the holotype with internal developing swarmer ventrally on segment 1 of basibiont (ZMB 12499). B. Paratype in side view ventrally on segment 3 of basibiont (ZMB 12489). Arrow marks apical cleft within lorica. Abbreviations: ca, basal cavity in lorica; lo, lorica; ma, macronucleus; mi, micronucleus; st, stalk with internal striation (information taken from different specimen); sw, swarmer; te, tentacle; zo, zooid. Scale bar 30 µm." figureDoi="http://doi.org/10.5281/zenodo.8345483" httpUri="https://zenodo.org/record/8345483/files/figure.png" pageId="9" pageNumber="448">Figs 2</figureCitation>
|
||
,
|
||
<figureCitation id="E6C62A1B990AFF9AFF50FDF17424FDF5" box="[244,308,583,610]" captionStart="FIGURE 4" captionStartId="10.[151,250,1749,1773]" captionTargetBox="[152,1436,181,1725]" captionTargetId="figure-24@10.[152,1436,181,1725]" captionTargetPageId="10" captionText="FIGURE 4. Setaphyes kielensis (A, ZMB 12469; B, ZMB 12489; C, ZMB 12486; D, E, ZMB 12492; F, ZMB 12488) with specimens of Trematosoma husselae sp. nov., SEM (A), and DIC (B–F). A. Segment 1, empty shells of epibionts, ventral view. B. Segments 1–11, optical section. C. Epibionts with capitated tentacles arranged in left and right row. Extended focus image of three images. D. Epibiont with developing swarmer. Arrowheads mark membrane of swarmer. E. Epibiont with swarmer recently freed from its parent. F. Segments 4–6, ventral view. Note thickened lorica in basal two thirds of epibionts.Arrow marks longitudinal striation of stalk of epibiont. Abbreviations: ca, basal cavity in lorica; co, specimen of Cothurnia buetschlii; lo, lorica; lts, lateral terminal spine; ma, macronucleus; mi, micronucleus; s, segment, followed by segment number; su, suctorian epibiont; sw, swarmer; te, tentacle. Scale bar in A, D, E 20 µm, in B 100 µm, in C 30 µm, and in F 50 µm." figureDoi="http://doi.org/10.5281/zenodo.8345487" httpUri="https://zenodo.org/record/8345487/files/figure.png" pageId="9" pageNumber="448">4D, F</figureCitation>
|
||
,
|
||
<figureCitation id="E6C62A1B990AFF9AFE9BFDF17493FDF6" box="[319,387,583,610]" captionStart="FIGURE 5" captionStartId="12.[151,250,186,210]" captionTargetId="figure-17@11.[151,1436,181,1725]" captionTargetPageId="11" captionText="FIGURE 5. Setaphyes kielensis (A, ZMB 12489; B, ZMB 12500; C, ZMB 12499; D, ZMB 12487; J, ZMB 12486; L, ZMB 12397; M, ZMB 12369) and S. dentatus (E–G, K, ZMB 12503; F, H, I, ZMB 12494) with specimens of Trematosoma husselae sp. nov. (A–G, J–M), Cothurnia buetschlii (J), and a case of fungus or developmental artefact (H, I, ZMB 12494), DIC. A. Segments 1–4, lateral and dorso-ventral view on the calyciform, dorso-ventrally compressed lorica with the zooid, ventrolateral view on basibiont. B. Epibiont with swarmer, stalk, and lorica in optical section. C. Epibiont with two macronuclei not fusing at any focal level. D, E. Young, small, spherical epibionts with few tentacles, not all tentacles in focus. F. Young specimen on cuticle of basibiont with striation pattern on surface (arrows). G. Epibiont with tentacles in empty shell of suctorian epibiont. H, I. Segment 10, dorsally (H) and optical section (I), subcuticular fungus or internal sclerotisation artefact of the cuticle (arrows). J. Assemblage of epibionts on segment 10 of basibiont with C. buetschlii (contracted specimen left, extended specimen right), T. husselae sp. nov., and two kinds of filamentous bacteria. Arrows mark endostyle. K. Suctorian on segment 7 of S. dentatus. L, M. Specimens collected 1998, showing cellular substructures (L) and withdrawn zooid with minimum amount of cellular substructures (M). Extended focus images of six (D), eight (H, L), and three (K) images. Arrowheads mark stalks in B, D, E, G, and J. Abbreviations: ba, bacteria; ca, basal cavity in lorica; co, C. buetschlii; en, endostyle; lo, lorica; ma, macronucleus; s, segment, followed by segment number; sc, spherical cavity; sp, sperm; st, stalk; su, suctorian epibiont; sw, swarmer; te, tentacle; tu, tube on segment 2. Scale bar in A 50 µm, in B 20 µm, valid for B, G–J and M, in C 20 µm, valid for C, K, and L, and in D–F 10 µm." figureDoi="http://doi.org/10.5281/zenodo.8349223" httpUri="https://zenodo.org/record/8349223/files/figure.png" pageId="9" pageNumber="448">5B, L</figureCitation>
|
||
). The stalk (length 5–7 µm, width 3–4 µm) contained few longitudinal striations recognisable only in favourable light microscopical specimens (
|
||
<figureCitation id="E6C62A1B990AFF9AFD62FDDD760CFD12" box="[710,796,619,645]" captionStart="FIGURE 4" captionStartId="10.[151,250,1749,1773]" captionTargetBox="[152,1436,181,1725]" captionTargetId="figure-24@10.[152,1436,181,1725]" captionTargetPageId="10" captionText="FIGURE 4. Setaphyes kielensis (A, ZMB 12469; B, ZMB 12489; C, ZMB 12486; D, E, ZMB 12492; F, ZMB 12488) with specimens of Trematosoma husselae sp. nov., SEM (A), and DIC (B–F). A. Segment 1, empty shells of epibionts, ventral view. B. Segments 1–11, optical section. C. Epibionts with capitated tentacles arranged in left and right row. Extended focus image of three images. D. Epibiont with developing swarmer. Arrowheads mark membrane of swarmer. E. Epibiont with swarmer recently freed from its parent. F. Segments 4–6, ventral view. Note thickened lorica in basal two thirds of epibionts.Arrow marks longitudinal striation of stalk of epibiont. Abbreviations: ca, basal cavity in lorica; co, specimen of Cothurnia buetschlii; lo, lorica; lts, lateral terminal spine; ma, macronucleus; mi, micronucleus; s, segment, followed by segment number; su, suctorian epibiont; sw, swarmer; te, tentacle. Scale bar in A, D, E 20 µm, in B 100 µm, in C 30 µm, and in F 50 µm." figureDoi="http://doi.org/10.5281/zenodo.8345487" httpUri="https://zenodo.org/record/8345487/files/figure.png" pageId="9" pageNumber="448">Figs 4F</figureCitation>
|
||
,
|
||
<figureCitation id="E6C62A1B990AFF9AFC82FDDD765CFD11" box="[806,844,619,646]" captionStart="FIGURE 5" captionStartId="12.[151,250,186,210]" captionTargetId="figure-17@11.[151,1436,181,1725]" captionTargetPageId="11" captionText="FIGURE 5. Setaphyes kielensis (A, ZMB 12489; B, ZMB 12500; C, ZMB 12499; D, ZMB 12487; J, ZMB 12486; L, ZMB 12397; M, ZMB 12369) and S. dentatus (E–G, K, ZMB 12503; F, H, I, ZMB 12494) with specimens of Trematosoma husselae sp. nov. (A–G, J–M), Cothurnia buetschlii (J), and a case of fungus or developmental artefact (H, I, ZMB 12494), DIC. A. Segments 1–4, lateral and dorso-ventral view on the calyciform, dorso-ventrally compressed lorica with the zooid, ventrolateral view on basibiont. B. Epibiont with swarmer, stalk, and lorica in optical section. C. Epibiont with two macronuclei not fusing at any focal level. D, E. Young, small, spherical epibionts with few tentacles, not all tentacles in focus. F. Young specimen on cuticle of basibiont with striation pattern on surface (arrows). G. Epibiont with tentacles in empty shell of suctorian epibiont. H, I. Segment 10, dorsally (H) and optical section (I), subcuticular fungus or internal sclerotisation artefact of the cuticle (arrows). J. Assemblage of epibionts on segment 10 of basibiont with C. buetschlii (contracted specimen left, extended specimen right), T. husselae sp. nov., and two kinds of filamentous bacteria. Arrows mark endostyle. K. Suctorian on segment 7 of S. dentatus. L, M. Specimens collected 1998, showing cellular substructures (L) and withdrawn zooid with minimum amount of cellular substructures (M). Extended focus images of six (D), eight (H, L), and three (K) images. Arrowheads mark stalks in B, D, E, G, and J. Abbreviations: ba, bacteria; ca, basal cavity in lorica; co, C. buetschlii; en, endostyle; lo, lorica; ma, macronucleus; s, segment, followed by segment number; sc, spherical cavity; sp, sperm; st, stalk; su, suctorian epibiont; sw, swarmer; te, tentacle; tu, tube on segment 2. Scale bar in A 50 µm, in B 20 µm, valid for B, G–J and M, in C 20 µm, valid for C, K, and L, and in D–F 10 µm." figureDoi="http://doi.org/10.5281/zenodo.8349223" httpUri="https://zenodo.org/record/8349223/files/figure.png" pageId="9" pageNumber="448">5G</figureCitation>
|
||
). The apical edge appeared more pronounced in SEM mounts and sometimes more rounded in light microscopical preparations depending on the degree of flattening on the slide (comp.
|
||
<figureCitation id="E6C62A1B990AFF9AFEF3FD05748FFD5A" box="[343,415,691,717]" captionStart="FIGURE 3" captionStartId="8.[151,250,1750,1774]" captionTargetBox="[151,1436,181,1726]" captionTargetId="figure-24@8.[151,1436,181,1726]" captionTargetPageId="8" captionText="FIGURE 3. Setaphyes kielensis (A–C, ZMB 12475; D, ZMB 12464; E, F, ZMB 12465; G, ZMB 12462) with specimens of Trematosoma husselae sp. nov., SEM, ventral view. A–C. Specimen with 43 epibionts, segments 1–4 (A), 5–7 (B), and 8–11 (C). D. Segment 8, epibiont surface. E. Segment 6, epibiont surface covered with detritus. F. Segment 8, frontal view on small spherical specimen of Trematosoma husselae sp. nov. G. Segment 10, arrow marks specimen with withdrawn tentacles. Abbreviations: gco, gland cell outlet; lts, lateral terminal spine; s, segment, followed by segment number; se, seta; su, suctorian epibiont; te, tentacle. Scale bar in A–C 40 µm, in D–F 10 µm, and in G 20 µm." figureDoi="http://doi.org/10.5281/zenodo.8345485" httpUri="https://zenodo.org/record/8345485/files/figure.png" pageId="9" pageNumber="448">Figs 3</figureCitation>
|
||
,
|
||
<figureCitation id="E6C62A1B990AFF9AFE09FD0274ABFD59" box="[429,443,692,718]" captionStart="FIGURE 7" captionStartId="14.[151,250,1739,1763]" captionTargetBox="[151,1436,181,1715]" captionTargetId="figure-24@14.[151,1436,181,1715]" captionTargetPageId="14" captionText="FIGURE 7. Setaphyes kielensis (ZMB 12468) with epibionts, SEM. A. Segments 1–11, ventral-right. B. Segments 4–8, ventral view. Arrows mark laterally recessed anterior margin of lorica of Cothurnia buetschlii. Abbreviations: co, Cothurnia buetschlii; fp, filamentous prokaryotes; lts, lateral terminal spine; s, segment, followed by segment number; se, seta; su, suctorian epibiont; tu, tube. Scale bar in A 100 µm and in B 20 µm." figureDoi="http://doi.org/10.5281/zenodo.8345491" httpUri="https://zenodo.org/record/8345491/files/figure.png" pageId="9" pageNumber="448">7</figureCitation>
|
||
and
|
||
<figureCitation id="E6C62A1B990AFF9AFE51FD057713FD5A" box="[501,515,691,717]" captionStart="FIGURE 8" captionStartId="15.[151,250,1745,1769]" captionTargetBox="[151,1436,182,1721]" captionTargetId="figure-17@15.[151,1436,181,1721]" captionTargetPageId="15" captionText="FIGURE 8. Setaphyes kielensis (A, ZMB 12468; B, ZMB 12474) with epibionts, SEM. A. Segment 6, left side. Arrow marks laterally recessed anterior margin of lorica of Cothurnia buetschlii. B. Segments 8–10, dorsal view.Abbreviations: co, Cothurnia buetschlii; fp, filamentous prokaryotes; s, segment, followed by segment number; su, suctorian epibiont; te, tentacles. Scale bar in A 10 µm and in B 20 µm." figureDoi="http://doi.org/10.5281/zenodo.8345493" httpUri="https://zenodo.org/record/8345493/files/figure.png" pageId="9" pageNumber="448">8</figureCitation>
|
||
with
|
||
<figureCitation id="E6C62A1B990AFF9AFDE2FD0577B3FD5A" box="[582,675,691,717]" captionStart="FIGURE 2" captionStartId="7.[151,250,913,937]" captionTargetBox="[253,1340,184,889]" captionTargetId="figure-17@7.[248,1340,181,889]" captionTargetPageId="7" captionText="FIGURE 2. Trematosoma husselae sp. nov. on Setaphyes kielensis. A. Drawing of the holotype with internal developing swarmer ventrally on segment 1 of basibiont (ZMB 12499). B. Paratype in side view ventrally on segment 3 of basibiont (ZMB 12489). Arrow marks apical cleft within lorica. Abbreviations: ca, basal cavity in lorica; lo, lorica; ma, macronucleus; mi, micronucleus; st, stalk with internal striation (information taken from different specimen); sw, swarmer; te, tentacle; zo, zooid. Scale bar 30 µm." figureDoi="http://doi.org/10.5281/zenodo.8345483" httpUri="https://zenodo.org/record/8345483/files/figure.png" pageId="9" pageNumber="448">Figs 2A</figureCitation>
|
||
,
|
||
<figureCitation id="E6C62A1B990AFF9AFD15FD0577FFFD5A" box="[689,751,691,717]" captionStart="FIGURE 4" captionStartId="10.[151,250,1749,1773]" captionTargetBox="[152,1436,181,1725]" captionTargetId="figure-24@10.[152,1436,181,1725]" captionTargetPageId="10" captionText="FIGURE 4. Setaphyes kielensis (A, ZMB 12469; B, ZMB 12489; C, ZMB 12486; D, E, ZMB 12492; F, ZMB 12488) with specimens of Trematosoma husselae sp. nov., SEM (A), and DIC (B–F). A. Segment 1, empty shells of epibionts, ventral view. B. Segments 1–11, optical section. C. Epibionts with capitated tentacles arranged in left and right row. Extended focus image of three images. D. Epibiont with developing swarmer. Arrowheads mark membrane of swarmer. E. Epibiont with swarmer recently freed from its parent. F. Segments 4–6, ventral view. Note thickened lorica in basal two thirds of epibionts.Arrow marks longitudinal striation of stalk of epibiont. Abbreviations: ca, basal cavity in lorica; co, specimen of Cothurnia buetschlii; lo, lorica; lts, lateral terminal spine; ma, macronucleus; mi, micronucleus; s, segment, followed by segment number; su, suctorian epibiont; sw, swarmer; te, tentacle. Scale bar in A, D, E 20 µm, in B 100 µm, in C 30 µm, and in F 50 µm." figureDoi="http://doi.org/10.5281/zenodo.8345487" httpUri="https://zenodo.org/record/8345487/files/figure.png" pageId="9" pageNumber="448">4B–F</figureCitation>
|
||
and
|
||
<figureCitation id="E6C62A1B990AFF9AFC8DFD05765FFD59" box="[809,847,691,718]" captionStart="FIGURE 5" captionStartId="12.[151,250,186,210]" captionTargetId="figure-17@11.[151,1436,181,1725]" captionTargetPageId="11" captionText="FIGURE 5. Setaphyes kielensis (A, ZMB 12489; B, ZMB 12500; C, ZMB 12499; D, ZMB 12487; J, ZMB 12486; L, ZMB 12397; M, ZMB 12369) and S. dentatus (E–G, K, ZMB 12503; F, H, I, ZMB 12494) with specimens of Trematosoma husselae sp. nov. (A–G, J–M), Cothurnia buetschlii (J), and a case of fungus or developmental artefact (H, I, ZMB 12494), DIC. A. Segments 1–4, lateral and dorso-ventral view on the calyciform, dorso-ventrally compressed lorica with the zooid, ventrolateral view on basibiont. B. Epibiont with swarmer, stalk, and lorica in optical section. C. Epibiont with two macronuclei not fusing at any focal level. D, E. Young, small, spherical epibionts with few tentacles, not all tentacles in focus. F. Young specimen on cuticle of basibiont with striation pattern on surface (arrows). G. Epibiont with tentacles in empty shell of suctorian epibiont. H, I. Segment 10, dorsally (H) and optical section (I), subcuticular fungus or internal sclerotisation artefact of the cuticle (arrows). J. Assemblage of epibionts on segment 10 of basibiont with C. buetschlii (contracted specimen left, extended specimen right), T. husselae sp. nov., and two kinds of filamentous bacteria. Arrows mark endostyle. K. Suctorian on segment 7 of S. dentatus. L, M. Specimens collected 1998, showing cellular substructures (L) and withdrawn zooid with minimum amount of cellular substructures (M). Extended focus images of six (D), eight (H, L), and three (K) images. Arrowheads mark stalks in B, D, E, G, and J. Abbreviations: ba, bacteria; ca, basal cavity in lorica; co, C. buetschlii; en, endostyle; lo, lorica; ma, macronucleus; s, segment, followed by segment number; sc, spherical cavity; sp, sperm; st, stalk; su, suctorian epibiont; sw, swarmer; te, tentacle; tu, tube on segment 2. Scale bar in A 50 µm, in B 20 µm, valid for B, G–J and M, in C 20 µm, valid for C, K, and L, and in D–F 10 µm." figureDoi="http://doi.org/10.5281/zenodo.8349223" httpUri="https://zenodo.org/record/8349223/files/figure.png" pageId="9" pageNumber="448">5A</figureCitation>
|
||
). The surface of the lorica showed slightly elevated circular rings at irregular intervals around the body in SEM (
|
||
<figureCitation id="E6C62A1B990AFF9AFC8AFD617699FD66" box="[814,905,727,753]" captionStart="FIGURE 3" captionStartId="8.[151,250,1750,1774]" captionTargetBox="[151,1436,181,1726]" captionTargetId="figure-24@8.[151,1436,181,1726]" captionTargetPageId="8" captionText="FIGURE 3. Setaphyes kielensis (A–C, ZMB 12475; D, ZMB 12464; E, F, ZMB 12465; G, ZMB 12462) with specimens of Trematosoma husselae sp. nov., SEM, ventral view. A–C. Specimen with 43 epibionts, segments 1–4 (A), 5–7 (B), and 8–11 (C). D. Segment 8, epibiont surface. E. Segment 6, epibiont surface covered with detritus. F. Segment 8, frontal view on small spherical specimen of Trematosoma husselae sp. nov. G. Segment 10, arrow marks specimen with withdrawn tentacles. Abbreviations: gco, gland cell outlet; lts, lateral terminal spine; s, segment, followed by segment number; se, seta; su, suctorian epibiont; te, tentacle. Scale bar in A–C 40 µm, in D–F 10 µm, and in G 20 µm." figureDoi="http://doi.org/10.5281/zenodo.8345485" httpUri="https://zenodo.org/record/8345485/files/figure.png" pageId="9" pageNumber="448">Figs 3D</figureCitation>
|
||
,
|
||
<figureCitation id="E6C62A1B990AFF9AFC37FD6176A9FD66" box="[915,953,727,753]" captionStart="FIGURE 8" captionStartId="15.[151,250,1745,1769]" captionTargetBox="[151,1436,182,1721]" captionTargetId="figure-17@15.[151,1436,181,1721]" captionTargetPageId="15" captionText="FIGURE 8. Setaphyes kielensis (A, ZMB 12468; B, ZMB 12474) with epibionts, SEM. A. Segment 6, left side. Arrow marks laterally recessed anterior margin of lorica of Cothurnia buetschlii. B. Segments 8–10, dorsal view.Abbreviations: co, Cothurnia buetschlii; fp, filamentous prokaryotes; s, segment, followed by segment number; su, suctorian epibiont; te, tentacles. Scale bar in A 10 µm and in B 20 µm." figureDoi="http://doi.org/10.5281/zenodo.8345493" httpUri="https://zenodo.org/record/8345493/files/figure.png" pageId="9" pageNumber="448">8A</figureCitation>
|
||
). In optical section, the lorica wall appeared much thicker (about 4 µm) in its basal two thirds than apically (
|
||
<figureCitation id="E6C62A1B990AFF9AFCF9FD4D76B6FC82" box="[861,934,763,789]" captionStart="FIGURE 2" captionStartId="7.[151,250,913,937]" captionTargetBox="[253,1340,184,889]" captionTargetId="figure-17@7.[248,1340,181,889]" captionTargetPageId="7" captionText="FIGURE 2. Trematosoma husselae sp. nov. on Setaphyes kielensis. A. Drawing of the holotype with internal developing swarmer ventrally on segment 1 of basibiont (ZMB 12499). B. Paratype in side view ventrally on segment 3 of basibiont (ZMB 12489). Arrow marks apical cleft within lorica. Abbreviations: ca, basal cavity in lorica; lo, lorica; ma, macronucleus; mi, micronucleus; st, stalk with internal striation (information taken from different specimen); sw, swarmer; te, tentacle; zo, zooid. Scale bar 30 µm." figureDoi="http://doi.org/10.5281/zenodo.8345483" httpUri="https://zenodo.org/record/8345483/files/figure.png" pageId="9" pageNumber="448">Figs 2</figureCitation>
|
||
,
|
||
<figureCitation id="E6C62A1B990AFF9AFC15FD4D76E4FC82" box="[945,1012,763,789]" captionStart="FIGURE 4" captionStartId="10.[151,250,1749,1773]" captionTargetBox="[152,1436,181,1725]" captionTargetId="figure-24@10.[152,1436,181,1725]" captionTargetPageId="10" captionText="FIGURE 4. Setaphyes kielensis (A, ZMB 12469; B, ZMB 12489; C, ZMB 12486; D, E, ZMB 12492; F, ZMB 12488) with specimens of Trematosoma husselae sp. nov., SEM (A), and DIC (B–F). A. Segment 1, empty shells of epibionts, ventral view. B. Segments 1–11, optical section. C. Epibionts with capitated tentacles arranged in left and right row. Extended focus image of three images. D. Epibiont with developing swarmer. Arrowheads mark membrane of swarmer. E. Epibiont with swarmer recently freed from its parent. F. Segments 4–6, ventral view. Note thickened lorica in basal two thirds of epibionts.Arrow marks longitudinal striation of stalk of epibiont. Abbreviations: ca, basal cavity in lorica; co, specimen of Cothurnia buetschlii; lo, lorica; lts, lateral terminal spine; ma, macronucleus; mi, micronucleus; s, segment, followed by segment number; su, suctorian epibiont; sw, swarmer; te, tentacle. Scale bar in A, D, E 20 µm, in B 100 µm, in C 30 µm, and in F 50 µm." figureDoi="http://doi.org/10.5281/zenodo.8345487" httpUri="https://zenodo.org/record/8345487/files/figure.png" pageId="9" pageNumber="448">4C–F</figureCitation>
|
||
). The tentacles (length 4–6 µm) could be withdrawn, and the terminal end of the lorica revealed a cleft (
|
||
<figureCitation id="E6C62A1B990AFF9AFCD8FCA976C9FCAE" box="[892,985,799,825]" captionStart="FIGURE 2" captionStartId="7.[151,250,913,937]" captionTargetBox="[253,1340,184,889]" captionTargetId="figure-17@7.[248,1340,181,889]" captionTargetPageId="7" captionText="FIGURE 2. Trematosoma husselae sp. nov. on Setaphyes kielensis. A. Drawing of the holotype with internal developing swarmer ventrally on segment 1 of basibiont (ZMB 12499). B. Paratype in side view ventrally on segment 3 of basibiont (ZMB 12489). Arrow marks apical cleft within lorica. Abbreviations: ca, basal cavity in lorica; lo, lorica; ma, macronucleus; mi, micronucleus; st, stalk with internal striation (information taken from different specimen); sw, swarmer; te, tentacle; zo, zooid. Scale bar 30 µm." figureDoi="http://doi.org/10.5281/zenodo.8345483" httpUri="https://zenodo.org/record/8345483/files/figure.png" pageId="9" pageNumber="448">Figs 2B</figureCitation>
|
||
,
|
||
<figureCitation id="E6C62A1B990AFF9AFC41FCA97119FCAE" box="[997,1033,799,825]" captionStart="FIGURE 3" captionStartId="8.[151,250,1750,1774]" captionTargetBox="[151,1436,181,1726]" captionTargetId="figure-24@8.[151,1436,181,1726]" captionTargetPageId="8" captionText="FIGURE 3. Setaphyes kielensis (A–C, ZMB 12475; D, ZMB 12464; E, F, ZMB 12465; G, ZMB 12462) with specimens of Trematosoma husselae sp. nov., SEM, ventral view. A–C. Specimen with 43 epibionts, segments 1–4 (A), 5–7 (B), and 8–11 (C). D. Segment 8, epibiont surface. E. Segment 6, epibiont surface covered with detritus. F. Segment 8, frontal view on small spherical specimen of Trematosoma husselae sp. nov. G. Segment 10, arrow marks specimen with withdrawn tentacles. Abbreviations: gco, gland cell outlet; lts, lateral terminal spine; s, segment, followed by segment number; se, seta; su, suctorian epibiont; te, tentacle. Scale bar in A–C 40 µm, in D–F 10 µm, and in G 20 µm." figureDoi="http://doi.org/10.5281/zenodo.8345485" httpUri="https://zenodo.org/record/8345485/files/figure.png" pageId="9" pageNumber="448">3G</figureCitation>
|
||
,
|
||
<figureCitation id="E6C62A1B990AFF9AFBB2FCA9712CFCAE" box="[1046,1084,799,825]" captionStart="FIGURE 4" captionStartId="10.[151,250,1749,1773]" captionTargetBox="[152,1436,181,1725]" captionTargetId="figure-24@10.[152,1436,181,1725]" captionTargetPageId="10" captionText="FIGURE 4. Setaphyes kielensis (A, ZMB 12469; B, ZMB 12489; C, ZMB 12486; D, E, ZMB 12492; F, ZMB 12488) with specimens of Trematosoma husselae sp. nov., SEM (A), and DIC (B–F). A. Segment 1, empty shells of epibionts, ventral view. B. Segments 1–11, optical section. C. Epibionts with capitated tentacles arranged in left and right row. Extended focus image of three images. D. Epibiont with developing swarmer. Arrowheads mark membrane of swarmer. E. Epibiont with swarmer recently freed from its parent. F. Segments 4–6, ventral view. Note thickened lorica in basal two thirds of epibionts.Arrow marks longitudinal striation of stalk of epibiont. Abbreviations: ca, basal cavity in lorica; co, specimen of Cothurnia buetschlii; lo, lorica; lts, lateral terminal spine; ma, macronucleus; mi, micronucleus; s, segment, followed by segment number; su, suctorian epibiont; sw, swarmer; te, tentacle. Scale bar in A, D, E 20 µm, in B 100 µm, in C 30 µm, and in F 50 µm." figureDoi="http://doi.org/10.5281/zenodo.8345487" httpUri="https://zenodo.org/record/8345487/files/figure.png" pageId="9" pageNumber="448">4A</figureCitation>
|
||
). The tentacles occurred in one left and one right row separated by a small gap on one of the broader sides of the zooid (
|
||
<figureCitation id="E6C62A1B990AFF9AFB28FCF571C6FCCA" box="[1164,1238,835,861]" captionStart="FIGURE 2" captionStartId="7.[151,250,913,937]" captionTargetBox="[253,1340,184,889]" captionTargetId="figure-17@7.[248,1340,181,889]" captionTargetPageId="7" captionText="FIGURE 2. Trematosoma husselae sp. nov. on Setaphyes kielensis. A. Drawing of the holotype with internal developing swarmer ventrally on segment 1 of basibiont (ZMB 12499). B. Paratype in side view ventrally on segment 3 of basibiont (ZMB 12489). Arrow marks apical cleft within lorica. Abbreviations: ca, basal cavity in lorica; lo, lorica; ma, macronucleus; mi, micronucleus; st, stalk with internal striation (information taken from different specimen); sw, swarmer; te, tentacle; zo, zooid. Scale bar 30 µm." figureDoi="http://doi.org/10.5281/zenodo.8345483" httpUri="https://zenodo.org/record/8345483/files/figure.png" pageId="9" pageNumber="448">Figs 2</figureCitation>
|
||
,
|
||
<figureCitation id="E6C62A1B990AFF9AFB47FCF57017FCCA" box="[1251,1287,835,861]" captionStart="FIGURE 4" captionStartId="10.[151,250,1749,1773]" captionTargetBox="[152,1436,181,1725]" captionTargetId="figure-24@10.[152,1436,181,1725]" captionTargetPageId="10" captionText="FIGURE 4. Setaphyes kielensis (A, ZMB 12469; B, ZMB 12489; C, ZMB 12486; D, E, ZMB 12492; F, ZMB 12488) with specimens of Trematosoma husselae sp. nov., SEM (A), and DIC (B–F). A. Segment 1, empty shells of epibionts, ventral view. B. Segments 1–11, optical section. C. Epibionts with capitated tentacles arranged in left and right row. Extended focus image of three images. D. Epibiont with developing swarmer. Arrowheads mark membrane of swarmer. E. Epibiont with swarmer recently freed from its parent. F. Segments 4–6, ventral view. Note thickened lorica in basal two thirds of epibionts.Arrow marks longitudinal striation of stalk of epibiont. Abbreviations: ca, basal cavity in lorica; co, specimen of Cothurnia buetschlii; lo, lorica; lts, lateral terminal spine; ma, macronucleus; mi, micronucleus; s, segment, followed by segment number; su, suctorian epibiont; sw, swarmer; te, tentacle. Scale bar in A, D, E 20 µm, in B 100 µm, in C 30 µm, and in F 50 µm." figureDoi="http://doi.org/10.5281/zenodo.8345487" httpUri="https://zenodo.org/record/8345487/files/figure.png" pageId="9" pageNumber="448">4C</figureCitation>
|
||
), but seemed sometimes to form a single row of tentacles (
|
||
<figureCitation id="E6C62A1B990AFF9AFD33FCD177CFFC16" box="[663,735,871,897]" captionStart="FIGURE 3" captionStartId="8.[151,250,1750,1774]" captionTargetBox="[151,1436,181,1726]" captionTargetId="figure-24@8.[151,1436,181,1726]" captionTargetPageId="8" captionText="FIGURE 3. Setaphyes kielensis (A–C, ZMB 12475; D, ZMB 12464; E, F, ZMB 12465; G, ZMB 12462) with specimens of Trematosoma husselae sp. nov., SEM, ventral view. A–C. Specimen with 43 epibionts, segments 1–4 (A), 5–7 (B), and 8–11 (C). D. Segment 8, epibiont surface. E. Segment 6, epibiont surface covered with detritus. F. Segment 8, frontal view on small spherical specimen of Trematosoma husselae sp. nov. G. Segment 10, arrow marks specimen with withdrawn tentacles. Abbreviations: gco, gland cell outlet; lts, lateral terminal spine; s, segment, followed by segment number; se, seta; su, suctorian epibiont; te, tentacle. Scale bar in A–C 40 µm, in D–F 10 µm, and in G 20 µm." figureDoi="http://doi.org/10.5281/zenodo.8345485" httpUri="https://zenodo.org/record/8345485/files/figure.png" pageId="9" pageNumber="448">
|
||
Figs
|
||
<geoCoordinate id="1BC95059990AFF9AFD74FCD177CFFC16" box="[720,735,871,897]" degrees="3, 4" direction="east" orientation="longitude" pageId="9" pageNumber="448" precision="5555" value="3.4">3</geoCoordinate>
|
||
</figureCitation>
|
||
,
|
||
<figureCitation id="E6C62A1B990AFF9AFD49FCD1761FFC16" box="[749,783,871,897]" captionStart="FIGURE 4" captionStartId="10.[151,250,1749,1773]" captionTargetBox="[152,1436,181,1725]" captionTargetId="figure-24@10.[152,1436,181,1725]" captionTargetPageId="10" captionText="FIGURE 4. Setaphyes kielensis (A, ZMB 12469; B, ZMB 12489; C, ZMB 12486; D, E, ZMB 12492; F, ZMB 12488) with specimens of Trematosoma husselae sp. nov., SEM (A), and DIC (B–F). A. Segment 1, empty shells of epibionts, ventral view. B. Segments 1–11, optical section. C. Epibionts with capitated tentacles arranged in left and right row. Extended focus image of three images. D. Epibiont with developing swarmer. Arrowheads mark membrane of swarmer. E. Epibiont with swarmer recently freed from its parent. F. Segments 4–6, ventral view. Note thickened lorica in basal two thirds of epibionts.Arrow marks longitudinal striation of stalk of epibiont. Abbreviations: ca, basal cavity in lorica; co, specimen of Cothurnia buetschlii; lo, lorica; lts, lateral terminal spine; ma, macronucleus; mi, micronucleus; s, segment, followed by segment number; su, suctorian epibiont; sw, swarmer; te, tentacle. Scale bar in A, D, E 20 µm, in B 100 µm, in C 30 µm, and in F 50 µm." figureDoi="http://doi.org/10.5281/zenodo.8345487" httpUri="https://zenodo.org/record/8345487/files/figure.png" pageId="9" pageNumber="448">4E</figureCitation>
|
||
,
|
||
<figureCitation id="E6C62A1B990AFF9AFCBFFCDE762EFC15" box="[795,830,872,898]" captionStart="FIGURE 7" captionStartId="14.[151,250,1739,1763]" captionTargetBox="[151,1436,181,1715]" captionTargetId="figure-24@14.[151,1436,181,1715]" captionTargetPageId="14" captionText="FIGURE 7. Setaphyes kielensis (ZMB 12468) with epibionts, SEM. A. Segments 1–11, ventral-right. B. Segments 4–8, ventral view. Arrows mark laterally recessed anterior margin of lorica of Cothurnia buetschlii. Abbreviations: co, Cothurnia buetschlii; fp, filamentous prokaryotes; lts, lateral terminal spine; s, segment, followed by segment number; se, seta; su, suctorian epibiont; tu, tube. Scale bar in A 100 µm and in B 20 µm." figureDoi="http://doi.org/10.5281/zenodo.8345491" httpUri="https://zenodo.org/record/8345491/files/figure.png" pageId="9" pageNumber="448">7B</figureCitation>
|
||
,
|
||
<figureCitation id="E6C62A1B990AFF9AFCEFFCD1764BFC16" box="[843,859,871,897]" captionStart="FIGURE 8" captionStartId="15.[151,250,1745,1769]" captionTargetBox="[151,1436,182,1721]" captionTargetId="figure-17@15.[151,1436,181,1721]" captionTargetPageId="15" captionText="FIGURE 8. Setaphyes kielensis (A, ZMB 12468; B, ZMB 12474) with epibionts, SEM. A. Segment 6, left side. Arrow marks laterally recessed anterior margin of lorica of Cothurnia buetschlii. B. Segments 8–10, dorsal view.Abbreviations: co, Cothurnia buetschlii; fp, filamentous prokaryotes; s, segment, followed by segment number; su, suctorian epibiont; te, tentacles. Scale bar in A 10 µm and in B 20 µm." figureDoi="http://doi.org/10.5281/zenodo.8345493" httpUri="https://zenodo.org/record/8345493/files/figure.png" pageId="9" pageNumber="448">8</figureCitation>
|
||
). An epibiont exhibited 8–16 tentacles in each row (
|
||
<figureCitation id="E6C62A1B990AFF9AFF3BFC3D75F6FC32" box="[159,230,907,933]" captionStart="FIGURE 2" captionStartId="7.[151,250,913,937]" captionTargetBox="[253,1340,184,889]" captionTargetId="figure-17@7.[248,1340,181,889]" captionTargetPageId="7" captionText="FIGURE 2. Trematosoma husselae sp. nov. on Setaphyes kielensis. A. Drawing of the holotype with internal developing swarmer ventrally on segment 1 of basibiont (ZMB 12499). B. Paratype in side view ventrally on segment 3 of basibiont (ZMB 12489). Arrow marks apical cleft within lorica. Abbreviations: ca, basal cavity in lorica; lo, lorica; ma, macronucleus; mi, micronucleus; st, stalk with internal striation (information taken from different specimen); sw, swarmer; te, tentacle; zo, zooid. Scale bar 30 µm." figureDoi="http://doi.org/10.5281/zenodo.8345483" httpUri="https://zenodo.org/record/8345483/files/figure.png" pageId="9" pageNumber="448">Figs 2</figureCitation>
|
||
,
|
||
<figureCitation id="E6C62A1B990AFF9AFF55FC3D743FFC32" box="[241,303,907,933]" captionStart="FIGURE 3" captionStartId="8.[151,250,1750,1774]" captionTargetBox="[151,1436,181,1726]" captionTargetId="figure-24@8.[151,1436,181,1726]" captionTargetPageId="8" captionText="FIGURE 3. Setaphyes kielensis (A–C, ZMB 12475; D, ZMB 12464; E, F, ZMB 12465; G, ZMB 12462) with specimens of Trematosoma husselae sp. nov., SEM, ventral view. A–C. Specimen with 43 epibionts, segments 1–4 (A), 5–7 (B), and 8–11 (C). D. Segment 8, epibiont surface. E. Segment 6, epibiont surface covered with detritus. F. Segment 8, frontal view on small spherical specimen of Trematosoma husselae sp. nov. G. Segment 10, arrow marks specimen with withdrawn tentacles. Abbreviations: gco, gland cell outlet; lts, lateral terminal spine; s, segment, followed by segment number; se, seta; su, suctorian epibiont; te, tentacle. Scale bar in A–C 40 µm, in D–F 10 µm, and in G 20 µm." figureDoi="http://doi.org/10.5281/zenodo.8345485" httpUri="https://zenodo.org/record/8345485/files/figure.png" pageId="9" pageNumber="448">3C–F</figureCitation>
|
||
,
|
||
<figureCitation id="E6C62A1B990AFF9AFE9DFC3D7484FC31" box="[313,404,907,934]" captionStart="FIGURE 4" captionStartId="10.[151,250,1749,1773]" captionTargetBox="[152,1436,181,1725]" captionTargetId="figure-24@10.[152,1436,181,1725]" captionTargetPageId="10" captionText="FIGURE 4. Setaphyes kielensis (A, ZMB 12469; B, ZMB 12489; C, ZMB 12486; D, E, ZMB 12492; F, ZMB 12488) with specimens of Trematosoma husselae sp. nov., SEM (A), and DIC (B–F). A. Segment 1, empty shells of epibionts, ventral view. B. Segments 1–11, optical section. C. Epibionts with capitated tentacles arranged in left and right row. Extended focus image of three images. D. Epibiont with developing swarmer. Arrowheads mark membrane of swarmer. E. Epibiont with swarmer recently freed from its parent. F. Segments 4–6, ventral view. Note thickened lorica in basal two thirds of epibionts.Arrow marks longitudinal striation of stalk of epibiont. Abbreviations: ca, basal cavity in lorica; co, specimen of Cothurnia buetschlii; lo, lorica; lts, lateral terminal spine; ma, macronucleus; mi, micronucleus; s, segment, followed by segment number; su, suctorian epibiont; sw, swarmer; te, tentacle. Scale bar in A, D, E 20 µm, in B 100 µm, in C 30 µm, and in F 50 µm." figureDoi="http://doi.org/10.5281/zenodo.8345487" httpUri="https://zenodo.org/record/8345487/files/figure.png" pageId="9" pageNumber="448">4C, E, F</figureCitation>
|
||
,
|
||
<figureCitation id="E6C62A1B990AFF9AFE3AFC3D74D4FC32" box="[414,452,907,933]" captionStart="FIGURE 8" captionStartId="15.[151,250,1745,1769]" captionTargetBox="[151,1436,182,1721]" captionTargetId="figure-17@15.[151,1436,181,1721]" captionTargetPageId="15" captionText="FIGURE 8. Setaphyes kielensis (A, ZMB 12468; B, ZMB 12474) with epibionts, SEM. A. Segment 6, left side. Arrow marks laterally recessed anterior margin of lorica of Cothurnia buetschlii. B. Segments 8–10, dorsal view.Abbreviations: co, Cothurnia buetschlii; fp, filamentous prokaryotes; s, segment, followed by segment number; su, suctorian epibiont; te, tentacles. Scale bar in A 10 µm and in B 20 µm." figureDoi="http://doi.org/10.5281/zenodo.8345493" httpUri="https://zenodo.org/record/8345493/files/figure.png" pageId="9" pageNumber="448">8A</figureCitation>
|
||
). Each capitate tentacle terminated in a small blunt knob with a minute central depression (
|
||
<figureCitation id="E6C62A1B990AFF9AFF3BFC1975F8FC5E" box="[159,232,943,969]" captionStart="FIGURE 2" captionStartId="7.[151,250,913,937]" captionTargetBox="[253,1340,184,889]" captionTargetId="figure-17@7.[248,1340,181,889]" captionTargetPageId="7" captionText="FIGURE 2. Trematosoma husselae sp. nov. on Setaphyes kielensis. A. Drawing of the holotype with internal developing swarmer ventrally on segment 1 of basibiont (ZMB 12499). B. Paratype in side view ventrally on segment 3 of basibiont (ZMB 12489). Arrow marks apical cleft within lorica. Abbreviations: ca, basal cavity in lorica; lo, lorica; ma, macronucleus; mi, micronucleus; st, stalk with internal striation (information taken from different specimen); sw, swarmer; te, tentacle; zo, zooid. Scale bar 30 µm." figureDoi="http://doi.org/10.5281/zenodo.8345483" httpUri="https://zenodo.org/record/8345483/files/figure.png" pageId="9" pageNumber="448">Figs 2</figureCitation>
|
||
,
|
||
<figureCitation id="E6C62A1B990AFF9AFF50FC197424FC5E" box="[244,308,943,969]" captionStart="FIGURE 3" captionStartId="8.[151,250,1750,1774]" captionTargetBox="[151,1436,181,1726]" captionTargetId="figure-24@8.[151,1436,181,1726]" captionTargetPageId="8" captionText="FIGURE 3. Setaphyes kielensis (A–C, ZMB 12475; D, ZMB 12464; E, F, ZMB 12465; G, ZMB 12462) with specimens of Trematosoma husselae sp. nov., SEM, ventral view. A–C. Specimen with 43 epibionts, segments 1–4 (A), 5–7 (B), and 8–11 (C). D. Segment 8, epibiont surface. E. Segment 6, epibiont surface covered with detritus. F. Segment 8, frontal view on small spherical specimen of Trematosoma husselae sp. nov. G. Segment 10, arrow marks specimen with withdrawn tentacles. Abbreviations: gco, gland cell outlet; lts, lateral terminal spine; s, segment, followed by segment number; se, seta; su, suctorian epibiont; te, tentacle. Scale bar in A–C 40 µm, in D–F 10 µm, and in G 20 µm." figureDoi="http://doi.org/10.5281/zenodo.8345485" httpUri="https://zenodo.org/record/8345485/files/figure.png" pageId="9" pageNumber="448">3D–F</figureCitation>
|
||
,
|
||
<figureCitation id="E6C62A1B990AFF9AFE9BFC197474FC5E" box="[319,356,943,969]" captionStart="FIGURE 4" captionStartId="10.[151,250,1749,1773]" captionTargetBox="[152,1436,181,1725]" captionTargetId="figure-24@10.[152,1436,181,1725]" captionTargetPageId="10" captionText="FIGURE 4. Setaphyes kielensis (A, ZMB 12469; B, ZMB 12489; C, ZMB 12486; D, E, ZMB 12492; F, ZMB 12488) with specimens of Trematosoma husselae sp. nov., SEM (A), and DIC (B–F). A. Segment 1, empty shells of epibionts, ventral view. B. Segments 1–11, optical section. C. Epibionts with capitated tentacles arranged in left and right row. Extended focus image of three images. D. Epibiont with developing swarmer. Arrowheads mark membrane of swarmer. E. Epibiont with swarmer recently freed from its parent. F. Segments 4–6, ventral view. Note thickened lorica in basal two thirds of epibionts.Arrow marks longitudinal striation of stalk of epibiont. Abbreviations: ca, basal cavity in lorica; co, specimen of Cothurnia buetschlii; lo, lorica; lts, lateral terminal spine; ma, macronucleus; mi, micronucleus; s, segment, followed by segment number; su, suctorian epibiont; sw, swarmer; te, tentacle. Scale bar in A, D, E 20 µm, in B 100 µm, in C 30 µm, and in F 50 µm." figureDoi="http://doi.org/10.5281/zenodo.8345487" httpUri="https://zenodo.org/record/8345487/files/figure.png" pageId="9" pageNumber="448">4C</figureCitation>
|
||
). Occasionally, empty shells of
|
||
<taxonomicName id="B9FD4D1D990AFF9AFD6CFC067653FC5E" authority="Neuhaus & Lu & Yamasaki & González-Casarrubios, 2023" authorityName="Neuhaus & Lu & Yamasaki & González-Casarrubios" authorityYear="2023" box="[712,835,943,969]" class="Kinetofragminophora" family="Acinetidae" genus="Trematosoma" higherTaxonomySource="GBIF" kingdom="Chromista" order="Suctorida" pageId="9" pageNumber="448" phylum="Ciliophora" rank="species" species="husselae" status="sp. nov.">
|
||
<emphasis id="4C89EA8C990AFF9AFD6CFC067653FC5E" box="[712,835,943,969]" italics="true" pageId="9" pageNumber="448">T. husselae</emphasis>
|
||
</taxonomicName>
|
||
<emphasis id="4C89EA8C990AFF9AFCEFFC1976B4FC5E" bold="true" box="[843,932,943,969]" pageId="9" pageNumber="448">
|
||
<taxonomicNameLabel id="57BA57F7990AFF9AFCEFFC1976B4FC5E" box="[843,932,943,969]" pageId="9" pageNumber="448" rank="species">sp. nov.</taxonomicNameLabel>
|
||
</emphasis>
|
||
were found (
|
||
<collectionCode id="18ECAE5B990AFF9AFB9DFC197167FC5E" box="[1081,1143,943,969]" country="Germany" httpUri="http://grbio.org/cool/8syf-d21i" name="Museum für Naturkunde Berlin (Zoological Collections)" pageId="9" pageNumber="448" type="Museum">ZMB</collectionCode>
|
||
12462, 12488;
|
||
<figureCitation id="E6C62A1B990AFF9AFA83FC197090FC5E" box="[1319,1408,943,969]" captionStart="FIGURE 4" captionStartId="10.[151,250,1749,1773]" captionTargetBox="[152,1436,181,1725]" captionTargetId="figure-24@10.[152,1436,181,1725]" captionTargetPageId="10" captionText="FIGURE 4. Setaphyes kielensis (A, ZMB 12469; B, ZMB 12489; C, ZMB 12486; D, E, ZMB 12492; F, ZMB 12488) with specimens of Trematosoma husselae sp. nov., SEM (A), and DIC (B–F). A. Segment 1, empty shells of epibionts, ventral view. B. Segments 1–11, optical section. C. Epibionts with capitated tentacles arranged in left and right row. Extended focus image of three images. D. Epibiont with developing swarmer. Arrowheads mark membrane of swarmer. E. Epibiont with swarmer recently freed from its parent. F. Segments 4–6, ventral view. Note thickened lorica in basal two thirds of epibionts.Arrow marks longitudinal striation of stalk of epibiont. Abbreviations: ca, basal cavity in lorica; co, specimen of Cothurnia buetschlii; lo, lorica; lts, lateral terminal spine; ma, macronucleus; mi, micronucleus; s, segment, followed by segment number; su, suctorian epibiont; sw, swarmer; te, tentacle. Scale bar in A, D, E 20 µm, in B 100 µm, in C 30 µm, and in F 50 µm." figureDoi="http://doi.org/10.5281/zenodo.8345487" httpUri="https://zenodo.org/record/8345487/files/figure.png" pageId="9" pageNumber="448">Fig. 4A</figureCitation>
|
||
).
|
||
</paragraph>
|
||
<paragraph id="7E42369E990AFF9AFF63FC6574C2FB36" blockId="9.[151,1437,475,1798]" pageId="9" pageNumber="448">
|
||
Specimens of
|
||
<taxonomicName id="B9FD4D1D990AFF9AFED6FC627767FC7A" authority="Neuhaus & Lu & Yamasaki & González-Casarrubios, 2023" authorityName="Neuhaus & Lu & Yamasaki & González-Casarrubios" authorityYear="2023" box="[370,631,979,1005]" class="Kinetofragminophora" family="Acinetidae" genus="Trematosoma" higherTaxonomySource="GBIF" kingdom="Chromista" order="Suctorida" pageId="9" pageNumber="448" phylum="Ciliophora" rank="species" species="husselae" status="sp. nov.">
|
||
<emphasis id="4C89EA8C990AFF9AFED6FC627767FC7A" box="[370,631,979,1005]" italics="true" pageId="9" pageNumber="448">Trematosoma husselae</emphasis>
|
||
</taxonomicName>
|
||
<emphasis id="4C89EA8C990AFF9AFD20FC6577F4FC7A" bold="true" box="[644,740,979,1005]" pageId="9" pageNumber="448">
|
||
<taxonomicNameLabel id="57BA57F7990AFF9AFD20FC6577F4FC7A" box="[644,740,979,1005]" pageId="9" pageNumber="448" rank="species">sp. nov.</taxonomicNameLabel>
|
||
</emphasis>
|
||
possessed usually one central spherical macronucleus and occasionally a more ovoid macronucleus or two spherical macronuclei, which did not fuse at a different focal level (
|
||
<collectionCode id="18ECAE5B990AFF9AFF3BFBAD75CDFBA2" box="[159,221,1051,1077]" country="Germany" httpUri="http://grbio.org/cool/8syf-d21i" name="Museum für Naturkunde Berlin (Zoological Collections)" pageId="9" pageNumber="448" type="Museum">ZMB</collectionCode>
|
||
12499;
|
||
<figureCitation id="E6C62A1B990AFF9AFE9FFBAD7485FBA1" box="[315,405,1051,1078]" captionStart="FIGURE 5" captionStartId="12.[151,250,186,210]" captionTargetId="figure-17@11.[151,1436,181,1725]" captionTargetPageId="11" captionText="FIGURE 5. Setaphyes kielensis (A, ZMB 12489; B, ZMB 12500; C, ZMB 12499; D, ZMB 12487; J, ZMB 12486; L, ZMB 12397; M, ZMB 12369) and S. dentatus (E–G, K, ZMB 12503; F, H, I, ZMB 12494) with specimens of Trematosoma husselae sp. nov. (A–G, J–M), Cothurnia buetschlii (J), and a case of fungus or developmental artefact (H, I, ZMB 12494), DIC. A. Segments 1–4, lateral and dorso-ventral view on the calyciform, dorso-ventrally compressed lorica with the zooid, ventrolateral view on basibiont. B. Epibiont with swarmer, stalk, and lorica in optical section. C. Epibiont with two macronuclei not fusing at any focal level. D, E. Young, small, spherical epibionts with few tentacles, not all tentacles in focus. F. Young specimen on cuticle of basibiont with striation pattern on surface (arrows). G. Epibiont with tentacles in empty shell of suctorian epibiont. H, I. Segment 10, dorsally (H) and optical section (I), subcuticular fungus or internal sclerotisation artefact of the cuticle (arrows). J. Assemblage of epibionts on segment 10 of basibiont with C. buetschlii (contracted specimen left, extended specimen right), T. husselae sp. nov., and two kinds of filamentous bacteria. Arrows mark endostyle. K. Suctorian on segment 7 of S. dentatus. L, M. Specimens collected 1998, showing cellular substructures (L) and withdrawn zooid with minimum amount of cellular substructures (M). Extended focus images of six (D), eight (H, L), and three (K) images. Arrowheads mark stalks in B, D, E, G, and J. Abbreviations: ba, bacteria; ca, basal cavity in lorica; co, C. buetschlii; en, endostyle; lo, lorica; ma, macronucleus; s, segment, followed by segment number; sc, spherical cavity; sp, sperm; st, stalk; su, suctorian epibiont; sw, swarmer; te, tentacle; tu, tube on segment 2. Scale bar in A 50 µm, in B 20 µm, valid for B, G–J and M, in C 20 µm, valid for C, K, and L, and in D–F 10 µm." figureDoi="http://doi.org/10.5281/zenodo.8349223" httpUri="https://zenodo.org/record/8349223/files/figure.png" pageId="9" pageNumber="448">Fig. 5C</figureCitation>
|
||
). In many cases, no micronuclei could be observed, but often 2–7 spherical micronuclei of the same yellowish colour as the macronucleus were found (e. g.,
|
||
<collectionCode id="18ECAE5B990AFF9AFC2AFBF676DBFBCD" box="[910,971,1088,1114]" country="Germany" httpUri="http://grbio.org/cool/8syf-d21i" name="Museum für Naturkunde Berlin (Zoological Collections)" pageId="9" pageNumber="448" type="Museum">ZMB</collectionCode>
|
||
12479, 12480, 12500;
|
||
<figureCitation id="E6C62A1B990AFF9AFB72FB89700FFBCE" box="[1238,1311,1087,1113]" captionStart="FIGURE 2" captionStartId="7.[151,250,913,937]" captionTargetBox="[253,1340,184,889]" captionTargetId="figure-17@7.[248,1340,181,889]" captionTargetPageId="7" captionText="FIGURE 2. Trematosoma husselae sp. nov. on Setaphyes kielensis. A. Drawing of the holotype with internal developing swarmer ventrally on segment 1 of basibiont (ZMB 12499). B. Paratype in side view ventrally on segment 3 of basibiont (ZMB 12489). Arrow marks apical cleft within lorica. Abbreviations: ca, basal cavity in lorica; lo, lorica; ma, macronucleus; mi, micronucleus; st, stalk with internal striation (information taken from different specimen); sw, swarmer; te, tentacle; zo, zooid. Scale bar 30 µm." figureDoi="http://doi.org/10.5281/zenodo.8345483" httpUri="https://zenodo.org/record/8345483/files/figure.png" pageId="9" pageNumber="448">Figs 2</figureCitation>
|
||
,
|
||
<figureCitation id="E6C62A1B990AFF9AFA89FB897066FBCE" box="[1325,1398,1087,1113]" captionStart="FIGURE 4" captionStartId="10.[151,250,1749,1773]" captionTargetBox="[152,1436,181,1725]" captionTargetId="figure-24@10.[152,1436,181,1725]" captionTargetPageId="10" captionText="FIGURE 4. Setaphyes kielensis (A, ZMB 12469; B, ZMB 12489; C, ZMB 12486; D, E, ZMB 12492; F, ZMB 12488) with specimens of Trematosoma husselae sp. nov., SEM (A), and DIC (B–F). A. Segment 1, empty shells of epibionts, ventral view. B. Segments 1–11, optical section. C. Epibionts with capitated tentacles arranged in left and right row. Extended focus image of three images. D. Epibiont with developing swarmer. Arrowheads mark membrane of swarmer. E. Epibiont with swarmer recently freed from its parent. F. Segments 4–6, ventral view. Note thickened lorica in basal two thirds of epibionts.Arrow marks longitudinal striation of stalk of epibiont. Abbreviations: ca, basal cavity in lorica; co, specimen of Cothurnia buetschlii; lo, lorica; lts, lateral terminal spine; ma, macronucleus; mi, micronucleus; s, segment, followed by segment number; su, suctorian epibiont; sw, swarmer; te, tentacle. Scale bar in A, D, E 20 µm, in B 100 µm, in C 30 µm, and in F 50 µm." figureDoi="http://doi.org/10.5281/zenodo.8345487" httpUri="https://zenodo.org/record/8345487/files/figure.png" pageId="9" pageNumber="448">4C, D</figureCitation>
|
||
). A contractile vacuole? may appear apically in an epibiont, but could only be traced in few specimens, possibly because of insufficient conservation.
|
||
</paragraph>
|
||
<paragraph id="7E42369E990AFF9AFF63FB1D77CFFA09" blockId="9.[151,1437,475,1798]" pageId="9" pageNumber="448">
|
||
Eleven epibionts harboured a single, inner, ovoid structure covered by two membranes, with a nucleus (
|
||
<collectionCode id="18ECAE5B990AFF9AFAFAFB1D708CFB52" box="[1374,1436,1195,1221]" country="Germany" httpUri="http://grbio.org/cool/8syf-d21i" name="Museum für Naturkunde Berlin (Zoological Collections)" pageId="9" pageNumber="448" type="Museum">ZMB</collectionCode>
|
||
12365, 12397, 12489, 12492, 12498, 12499, 12500;
|
||
<figureCitation id="E6C62A1B990AFF9AFD41FB797651FB7E" box="[741,833,1231,1257]" captionStart="FIGURE 4" captionStartId="10.[151,250,1749,1773]" captionTargetBox="[152,1436,181,1725]" captionTargetId="figure-24@10.[152,1436,181,1725]" captionTargetPageId="10" captionText="FIGURE 4. Setaphyes kielensis (A, ZMB 12469; B, ZMB 12489; C, ZMB 12486; D, E, ZMB 12492; F, ZMB 12488) with specimens of Trematosoma husselae sp. nov., SEM (A), and DIC (B–F). A. Segment 1, empty shells of epibionts, ventral view. B. Segments 1–11, optical section. C. Epibionts with capitated tentacles arranged in left and right row. Extended focus image of three images. D. Epibiont with developing swarmer. Arrowheads mark membrane of swarmer. E. Epibiont with swarmer recently freed from its parent. F. Segments 4–6, ventral view. Note thickened lorica in basal two thirds of epibionts.Arrow marks longitudinal striation of stalk of epibiont. Abbreviations: ca, basal cavity in lorica; co, specimen of Cothurnia buetschlii; lo, lorica; lts, lateral terminal spine; ma, macronucleus; mi, micronucleus; s, segment, followed by segment number; su, suctorian epibiont; sw, swarmer; te, tentacle. Scale bar in A, D, E 20 µm, in B 100 µm, in C 30 µm, and in F 50 µm." figureDoi="http://doi.org/10.5281/zenodo.8345487" httpUri="https://zenodo.org/record/8345487/files/figure.png" pageId="9" pageNumber="448">Figs 4D</figureCitation>
|
||
,
|
||
<figureCitation id="E6C62A1B990AFF9AFCE9FB797660FB7D" box="[845,880,1231,1258]" captionStart="FIGURE 5" captionStartId="12.[151,250,186,210]" captionTargetId="figure-17@11.[151,1436,181,1725]" captionTargetPageId="11" captionText="FIGURE 5. Setaphyes kielensis (A, ZMB 12489; B, ZMB 12500; C, ZMB 12499; D, ZMB 12487; J, ZMB 12486; L, ZMB 12397; M, ZMB 12369) and S. dentatus (E–G, K, ZMB 12503; F, H, I, ZMB 12494) with specimens of Trematosoma husselae sp. nov. (A–G, J–M), Cothurnia buetschlii (J), and a case of fungus or developmental artefact (H, I, ZMB 12494), DIC. A. Segments 1–4, lateral and dorso-ventral view on the calyciform, dorso-ventrally compressed lorica with the zooid, ventrolateral view on basibiont. B. Epibiont with swarmer, stalk, and lorica in optical section. C. Epibiont with two macronuclei not fusing at any focal level. D, E. Young, small, spherical epibionts with few tentacles, not all tentacles in focus. F. Young specimen on cuticle of basibiont with striation pattern on surface (arrows). G. Epibiont with tentacles in empty shell of suctorian epibiont. H, I. Segment 10, dorsally (H) and optical section (I), subcuticular fungus or internal sclerotisation artefact of the cuticle (arrows). J. Assemblage of epibionts on segment 10 of basibiont with C. buetschlii (contracted specimen left, extended specimen right), T. husselae sp. nov., and two kinds of filamentous bacteria. Arrows mark endostyle. K. Suctorian on segment 7 of S. dentatus. L, M. Specimens collected 1998, showing cellular substructures (L) and withdrawn zooid with minimum amount of cellular substructures (M). Extended focus images of six (D), eight (H, L), and three (K) images. Arrowheads mark stalks in B, D, E, G, and J. Abbreviations: ba, bacteria; ca, basal cavity in lorica; co, C. buetschlii; en, endostyle; lo, lorica; ma, macronucleus; s, segment, followed by segment number; sc, spherical cavity; sp, sperm; st, stalk; su, suctorian epibiont; sw, swarmer; te, tentacle; tu, tube on segment 2. Scale bar in A 50 µm, in B 20 µm, valid for B, G–J and M, in C 20 µm, valid for C, K, and L, and in D–F 10 µm." figureDoi="http://doi.org/10.5281/zenodo.8349223" httpUri="https://zenodo.org/record/8349223/files/figure.png" pageId="9" pageNumber="448">5B</figureCitation>
|
||
; 13–15 µm x 8–10 µm). This ovoid structure was interpreted as a developing swarmer.
|
||
<taxonomicName id="B9FD4D1D990AFF9AFD90FB427621FA9A" authority="Neuhaus & Lu & Yamasaki & González-Casarrubios, 2023" authorityName="Neuhaus & Lu & Yamasaki & González-Casarrubios" authorityYear="2023" box="[564,817,1267,1293]" class="Kinetofragminophora" family="Acinetidae" genus="Trematosoma" higherTaxonomySource="GBIF" kingdom="Chromista" order="Suctorida" pageId="9" pageNumber="448" phylum="Ciliophora" rank="species" species="husselae" status="sp. nov.">
|
||
<emphasis id="4C89EA8C990AFF9AFD90FB427621FA9A" box="[564,817,1267,1293]" italics="true" pageId="9" pageNumber="448">Trematosoma husselae</emphasis>
|
||
</taxonomicName>
|
||
<emphasis id="4C89EA8C990AFF9AFC93FB457680FA9A" bold="true" box="[823,912,1267,1293]" pageId="9" pageNumber="448">
|
||
<taxonomicNameLabel id="57BA57F7990AFF9AFC93FB457680FA9A" box="[823,912,1267,1293]" pageId="9" pageNumber="448" rank="species">sp. nov.</taxonomicNameLabel>
|
||
</emphasis>
|
||
seemed to develop from a significantly smaller spherical stage (
|
||
<figureCitation id="E6C62A1B990AFF9AFEEEFAA174D1FAA5" box="[330,449,1303,1330]" captionStart="FIGURE 5" captionStartId="12.[151,250,186,210]" captionTargetId="figure-17@11.[151,1436,181,1725]" captionTargetPageId="11" captionText="FIGURE 5. Setaphyes kielensis (A, ZMB 12489; B, ZMB 12500; C, ZMB 12499; D, ZMB 12487; J, ZMB 12486; L, ZMB 12397; M, ZMB 12369) and S. dentatus (E–G, K, ZMB 12503; F, H, I, ZMB 12494) with specimens of Trematosoma husselae sp. nov. (A–G, J–M), Cothurnia buetschlii (J), and a case of fungus or developmental artefact (H, I, ZMB 12494), DIC. A. Segments 1–4, lateral and dorso-ventral view on the calyciform, dorso-ventrally compressed lorica with the zooid, ventrolateral view on basibiont. B. Epibiont with swarmer, stalk, and lorica in optical section. C. Epibiont with two macronuclei not fusing at any focal level. D, E. Young, small, spherical epibionts with few tentacles, not all tentacles in focus. F. Young specimen on cuticle of basibiont with striation pattern on surface (arrows). G. Epibiont with tentacles in empty shell of suctorian epibiont. H, I. Segment 10, dorsally (H) and optical section (I), subcuticular fungus or internal sclerotisation artefact of the cuticle (arrows). J. Assemblage of epibionts on segment 10 of basibiont with C. buetschlii (contracted specimen left, extended specimen right), T. husselae sp. nov., and two kinds of filamentous bacteria. Arrows mark endostyle. K. Suctorian on segment 7 of S. dentatus. L, M. Specimens collected 1998, showing cellular substructures (L) and withdrawn zooid with minimum amount of cellular substructures (M). Extended focus images of six (D), eight (H, L), and three (K) images. Arrowheads mark stalks in B, D, E, G, and J. Abbreviations: ba, bacteria; ca, basal cavity in lorica; co, C. buetschlii; en, endostyle; lo, lorica; ma, macronucleus; s, segment, followed by segment number; sc, spherical cavity; sp, sperm; st, stalk; su, suctorian epibiont; sw, swarmer; te, tentacle; tu, tube on segment 2. Scale bar in A 50 µm, in B 20 µm, valid for B, G–J and M, in C 20 µm, valid for C, K, and L, and in D–F 10 µm." figureDoi="http://doi.org/10.5281/zenodo.8349223" httpUri="https://zenodo.org/record/8349223/files/figure.png" pageId="9" pageNumber="448">Fig. 5D, E</figureCitation>
|
||
; 12 µm x 10 µm) with a minimum of about four tentacles and a short stalk (3 µm x 2 µm) via small calyciform and laterally compressed stages to much larger calyciform and laterally compressed specimens with up to 25 tentales (comp.
|
||
<figureCitation id="E6C62A1B990AFF9AFE47FAE97745FAEE" box="[483,597,1375,1401]" captionStart="FIGURE 4" captionStartId="10.[151,250,1749,1773]" captionTargetBox="[152,1436,181,1725]" captionTargetId="figure-24@10.[152,1436,181,1725]" captionTargetPageId="10" captionText="FIGURE 4. Setaphyes kielensis (A, ZMB 12469; B, ZMB 12489; C, ZMB 12486; D, E, ZMB 12492; F, ZMB 12488) with specimens of Trematosoma husselae sp. nov., SEM (A), and DIC (B–F). A. Segment 1, empty shells of epibionts, ventral view. B. Segments 1–11, optical section. C. Epibionts with capitated tentacles arranged in left and right row. Extended focus image of three images. D. Epibiont with developing swarmer. Arrowheads mark membrane of swarmer. E. Epibiont with swarmer recently freed from its parent. F. Segments 4–6, ventral view. Note thickened lorica in basal two thirds of epibionts.Arrow marks longitudinal striation of stalk of epibiont. Abbreviations: ca, basal cavity in lorica; co, specimen of Cothurnia buetschlii; lo, lorica; lts, lateral terminal spine; ma, macronucleus; mi, micronucleus; s, segment, followed by segment number; su, suctorian epibiont; sw, swarmer; te, tentacle. Scale bar in A, D, E 20 µm, in B 100 µm, in C 30 µm, and in F 50 µm." figureDoi="http://doi.org/10.5281/zenodo.8345487" httpUri="https://zenodo.org/record/8345487/files/figure.png" pageId="9" pageNumber="448">Fig. 4C–F</figureCitation>
|
||
with
|
||
<figureCitation id="E6C62A1B990AFF9AFD30FAE9761CFAEE" box="[660,780,1375,1402]" captionStart="FIGURE 5" captionStartId="12.[151,250,186,210]" captionTargetId="figure-17@11.[151,1436,181,1725]" captionTargetPageId="11" captionText="FIGURE 5. Setaphyes kielensis (A, ZMB 12489; B, ZMB 12500; C, ZMB 12499; D, ZMB 12487; J, ZMB 12486; L, ZMB 12397; M, ZMB 12369) and S. dentatus (E–G, K, ZMB 12503; F, H, I, ZMB 12494) with specimens of Trematosoma husselae sp. nov. (A–G, J–M), Cothurnia buetschlii (J), and a case of fungus or developmental artefact (H, I, ZMB 12494), DIC. A. Segments 1–4, lateral and dorso-ventral view on the calyciform, dorso-ventrally compressed lorica with the zooid, ventrolateral view on basibiont. B. Epibiont with swarmer, stalk, and lorica in optical section. C. Epibiont with two macronuclei not fusing at any focal level. D, E. Young, small, spherical epibionts with few tentacles, not all tentacles in focus. F. Young specimen on cuticle of basibiont with striation pattern on surface (arrows). G. Epibiont with tentacles in empty shell of suctorian epibiont. H, I. Segment 10, dorsally (H) and optical section (I), subcuticular fungus or internal sclerotisation artefact of the cuticle (arrows). J. Assemblage of epibionts on segment 10 of basibiont with C. buetschlii (contracted specimen left, extended specimen right), T. husselae sp. nov., and two kinds of filamentous bacteria. Arrows mark endostyle. K. Suctorian on segment 7 of S. dentatus. L, M. Specimens collected 1998, showing cellular substructures (L) and withdrawn zooid with minimum amount of cellular substructures (M). Extended focus images of six (D), eight (H, L), and three (K) images. Arrowheads mark stalks in B, D, E, G, and J. Abbreviations: ba, bacteria; ca, basal cavity in lorica; co, C. buetschlii; en, endostyle; lo, lorica; ma, macronucleus; s, segment, followed by segment number; sc, spherical cavity; sp, sperm; st, stalk; su, suctorian epibiont; sw, swarmer; te, tentacle; tu, tube on segment 2. Scale bar in A 50 µm, in B 20 µm, valid for B, G–J and M, in C 20 µm, valid for C, K, and L, and in D–F 10 µm." figureDoi="http://doi.org/10.5281/zenodo.8349223" httpUri="https://zenodo.org/record/8349223/files/figure.png" pageId="9" pageNumber="448">Fig. 5D, E</figureCitation>
|
||
). One young attached specimen (16 µm x 13 µm) seems to possess parallel rows of surface structures (
|
||
<figureCitation id="E6C62A1B990AFF9AFDD9FA3577C3FA09" box="[637,723,1411,1438]" captionStart="FIGURE 5" captionStartId="12.[151,250,186,210]" captionTargetId="figure-17@11.[151,1436,181,1725]" captionTargetPageId="11" captionText="FIGURE 5. Setaphyes kielensis (A, ZMB 12489; B, ZMB 12500; C, ZMB 12499; D, ZMB 12487; J, ZMB 12486; L, ZMB 12397; M, ZMB 12369) and S. dentatus (E–G, K, ZMB 12503; F, H, I, ZMB 12494) with specimens of Trematosoma husselae sp. nov. (A–G, J–M), Cothurnia buetschlii (J), and a case of fungus or developmental artefact (H, I, ZMB 12494), DIC. A. Segments 1–4, lateral and dorso-ventral view on the calyciform, dorso-ventrally compressed lorica with the zooid, ventrolateral view on basibiont. B. Epibiont with swarmer, stalk, and lorica in optical section. C. Epibiont with two macronuclei not fusing at any focal level. D, E. Young, small, spherical epibionts with few tentacles, not all tentacles in focus. F. Young specimen on cuticle of basibiont with striation pattern on surface (arrows). G. Epibiont with tentacles in empty shell of suctorian epibiont. H, I. Segment 10, dorsally (H) and optical section (I), subcuticular fungus or internal sclerotisation artefact of the cuticle (arrows). J. Assemblage of epibionts on segment 10 of basibiont with C. buetschlii (contracted specimen left, extended specimen right), T. husselae sp. nov., and two kinds of filamentous bacteria. Arrows mark endostyle. K. Suctorian on segment 7 of S. dentatus. L, M. Specimens collected 1998, showing cellular substructures (L) and withdrawn zooid with minimum amount of cellular substructures (M). Extended focus images of six (D), eight (H, L), and three (K) images. Arrowheads mark stalks in B, D, E, G, and J. Abbreviations: ba, bacteria; ca, basal cavity in lorica; co, C. buetschlii; en, endostyle; lo, lorica; ma, macronucleus; s, segment, followed by segment number; sc, spherical cavity; sp, sperm; st, stalk; su, suctorian epibiont; sw, swarmer; te, tentacle; tu, tube on segment 2. Scale bar in A 50 µm, in B 20 µm, valid for B, G–J and M, in C 20 µm, valid for C, K, and L, and in D–F 10 µm." figureDoi="http://doi.org/10.5281/zenodo.8349223" httpUri="https://zenodo.org/record/8349223/files/figure.png" pageId="9" pageNumber="448">Fig. 5F</figureCitation>
|
||
).
|
||
</paragraph>
|
||
<paragraph id="7E42369E990AFF9AFF63FA1175EEF99E" blockId="9.[151,1437,475,1798]" pageId="9" pageNumber="448">
|
||
A small spherical structure with one macronucleus and two micronuclei seemed to be held by the tentacles of a specimen of
|
||
<taxonomicName id="B9FD4D1D990AFF9AFE9DFA7A74A4FA72" authority="Neuhaus & Lu & Yamasaki & González-Casarrubios, 2023" authorityName="Neuhaus & Lu & Yamasaki & González-Casarrubios" authorityYear="2023" box="[313,436,1483,1509]" class="Kinetofragminophora" family="Acinetidae" genus="Trematosoma" higherTaxonomySource="GBIF" kingdom="Chromista" order="Suctorida" pageId="9" pageNumber="448" phylum="Ciliophora" rank="species" species="husselae" status="sp. nov.">
|
||
<emphasis id="4C89EA8C990AFF9AFE9DFA7A74A4FA72" box="[313,436,1483,1509]" italics="true" pageId="9" pageNumber="448">T. husselae</emphasis>
|
||
</taxonomicName>
|
||
<emphasis id="4C89EA8C990AFF9AFE18FA7D7705FA72" bold="true" box="[444,533,1483,1509]" pageId="9" pageNumber="448">
|
||
<taxonomicNameLabel id="57BA57F7990AFF9AFE18FA7D7705FA72" box="[444,533,1483,1509]" pageId="9" pageNumber="448" rank="species">sp. nov.</taxonomicNameLabel>
|
||
</emphasis>
|
||
(
|
||
<collectionCode id="18ECAE5B990AFF9AFD87FA7D7771FA72" box="[547,609,1483,1509]" country="Germany" httpUri="http://grbio.org/cool/8syf-d21i" name="Museum für Naturkunde Berlin (Zoological Collections)" pageId="9" pageNumber="448" type="Museum">ZMB</collectionCode>
|
||
12492;
|
||
<figureCitation id="E6C62A1B990AFF9AFD19FA7D7604FA72" box="[701,788,1483,1509]" captionStart="FIGURE 4" captionStartId="10.[151,250,1749,1773]" captionTargetBox="[152,1436,181,1725]" captionTargetId="figure-24@10.[152,1436,181,1725]" captionTargetPageId="10" captionText="FIGURE 4. Setaphyes kielensis (A, ZMB 12469; B, ZMB 12489; C, ZMB 12486; D, E, ZMB 12492; F, ZMB 12488) with specimens of Trematosoma husselae sp. nov., SEM (A), and DIC (B–F). A. Segment 1, empty shells of epibionts, ventral view. B. Segments 1–11, optical section. C. Epibionts with capitated tentacles arranged in left and right row. Extended focus image of three images. D. Epibiont with developing swarmer. Arrowheads mark membrane of swarmer. E. Epibiont with swarmer recently freed from its parent. F. Segments 4–6, ventral view. Note thickened lorica in basal two thirds of epibionts.Arrow marks longitudinal striation of stalk of epibiont. Abbreviations: ca, basal cavity in lorica; co, specimen of Cothurnia buetschlii; lo, lorica; lts, lateral terminal spine; ma, macronucleus; mi, micronucleus; s, segment, followed by segment number; su, suctorian epibiont; sw, swarmer; te, tentacle. Scale bar in A, D, E 20 µm, in B 100 µm, in C 30 µm, and in F 50 µm." figureDoi="http://doi.org/10.5281/zenodo.8345487" httpUri="https://zenodo.org/record/8345487/files/figure.png" pageId="9" pageNumber="448">Fig. 4E</figureCitation>
|
||
). This was interpreted as a swarmer recently born from its trophont.
|
||
</paragraph>
|
||
<paragraph id="7E42369E990AFF9AFF63F9A5763BF9E2" blockId="9.[151,1437,475,1798]" pageId="9" pageNumber="448">
|
||
On
|
||
<specimenCount id="68FBFD17990AFF9AFF54F9A2749AF9BA" box="[240,394,1555,1582]" pageId="9" pageNumber="448" type="generic">one specimen</specimenCount>
|
||
on
|
||
<taxonomicName id="B9FD4D1D990AFF9AFE17F9A5773EF9BA" baseAuthorityName="Reinhard" baseAuthorityYear="1881" box="[435,558,1555,1581]" class="Allomalorhagida" family="Pycnophyidae" genus="Setaphyes" kingdom="Animalia" pageId="9" pageNumber="448" phylum="Kinorhyncha" rank="species" species="dentatus">
|
||
<emphasis id="4C89EA8C990AFF9AFE17F9A5773EF9BA" box="[435,558,1555,1581]" italics="true" pageId="9" pageNumber="448">S. dentatus</emphasis>
|
||
</taxonomicName>
|
||
, a small spherical
|
||
<taxonomicName id="B9FD4D1D990AFF9AFD5BF9A276EDF9BA" authority="Neuhaus & Lu & Yamasaki & González-Casarrubios, 2023" authorityName="Neuhaus & Lu & Yamasaki & González-Casarrubios" authorityYear="2023" box="[767,1021,1555,1581]" class="Kinetofragminophora" family="Acinetidae" genus="Trematosoma" higherTaxonomySource="GBIF" kingdom="Chromista" order="Suctorida" pageId="9" pageNumber="448" phylum="Ciliophora" rank="species" species="husselae" status="sp. nov.">
|
||
<emphasis id="4C89EA8C990AFF9AFD5BF9A276EDF9BA" box="[767,1021,1555,1581]" italics="true" pageId="9" pageNumber="448">Trematosoma husselae</emphasis>
|
||
</taxonomicName>
|
||
<emphasis id="4C89EA8C990AFF9AFBA0F9A5714EF9BA" bold="true" box="[1028,1118,1555,1581]" pageId="9" pageNumber="448">
|
||
<taxonomicNameLabel id="57BA57F7990AFF9AFBA0F9A5714EF9BA" box="[1028,1118,1555,1581]" pageId="9" pageNumber="448" rank="species">sp. nov.</taxonomicNameLabel>
|
||
</emphasis>
|
||
with few tentacles appeared inside the empty shell of a larger empty lorica (
|
||
<collectionCode id="18ECAE5B990AFF9AFD18F98177EAF9C6" box="[700,762,1591,1617]" country="Germany" httpUri="http://grbio.org/cool/8syf-d21i" name="Museum für Naturkunde Berlin (Zoological Collections)" pageId="9" pageNumber="448" type="Museum">ZMB</collectionCode>
|
||
12503;
|
||
<figureCitation id="E6C62A1B990AFF9AFCFFF98176A7F9C5" box="[859,951,1591,1618]" captionStart="FIGURE 5" captionStartId="12.[151,250,186,210]" captionTargetId="figure-17@11.[151,1436,181,1725]" captionTargetPageId="11" captionText="FIGURE 5. Setaphyes kielensis (A, ZMB 12489; B, ZMB 12500; C, ZMB 12499; D, ZMB 12487; J, ZMB 12486; L, ZMB 12397; M, ZMB 12369) and S. dentatus (E–G, K, ZMB 12503; F, H, I, ZMB 12494) with specimens of Trematosoma husselae sp. nov. (A–G, J–M), Cothurnia buetschlii (J), and a case of fungus or developmental artefact (H, I, ZMB 12494), DIC. A. Segments 1–4, lateral and dorso-ventral view on the calyciform, dorso-ventrally compressed lorica with the zooid, ventrolateral view on basibiont. B. Epibiont with swarmer, stalk, and lorica in optical section. C. Epibiont with two macronuclei not fusing at any focal level. D, E. Young, small, spherical epibionts with few tentacles, not all tentacles in focus. F. Young specimen on cuticle of basibiont with striation pattern on surface (arrows). G. Epibiont with tentacles in empty shell of suctorian epibiont. H, I. Segment 10, dorsally (H) and optical section (I), subcuticular fungus or internal sclerotisation artefact of the cuticle (arrows). J. Assemblage of epibionts on segment 10 of basibiont with C. buetschlii (contracted specimen left, extended specimen right), T. husselae sp. nov., and two kinds of filamentous bacteria. Arrows mark endostyle. K. Suctorian on segment 7 of S. dentatus. L, M. Specimens collected 1998, showing cellular substructures (L) and withdrawn zooid with minimum amount of cellular substructures (M). Extended focus images of six (D), eight (H, L), and three (K) images. Arrowheads mark stalks in B, D, E, G, and J. Abbreviations: ba, bacteria; ca, basal cavity in lorica; co, C. buetschlii; en, endostyle; lo, lorica; ma, macronucleus; s, segment, followed by segment number; sc, spherical cavity; sp, sperm; st, stalk; su, suctorian epibiont; sw, swarmer; te, tentacle; tu, tube on segment 2. Scale bar in A 50 µm, in B 20 µm, valid for B, G–J and M, in C 20 µm, valid for C, K, and L, and in D–F 10 µm." figureDoi="http://doi.org/10.5281/zenodo.8349223" httpUri="https://zenodo.org/record/8349223/files/figure.png" pageId="9" pageNumber="448">Fig. 5G</figureCitation>
|
||
). This specimen was probably re-arranged during mounting of the slide and is regarded as an artefact.
|
||
</paragraph>
|
||
<paragraph id="7E42369E990AFF9AFF63F9367164F891" blockId="9.[151,1437,475,1798]" pageId="9" pageNumber="448">
|
||
In specimens collected 1998, the cytoplasm was often found withdrawn from the base to the apical part of the lorica and revealed almost no substructures except the macronucleus and micronuclei; tentacles could usually not be traced (
|
||
<figureCitation id="E6C62A1B990AFF9AFF4EF9717458F975" box="[234,328,1735,1762]" captionStart="FIGURE 5" captionStartId="12.[151,250,186,210]" captionTargetId="figure-17@11.[151,1436,181,1725]" captionTargetPageId="11" captionText="FIGURE 5. Setaphyes kielensis (A, ZMB 12489; B, ZMB 12500; C, ZMB 12499; D, ZMB 12487; J, ZMB 12486; L, ZMB 12397; M, ZMB 12369) and S. dentatus (E–G, K, ZMB 12503; F, H, I, ZMB 12494) with specimens of Trematosoma husselae sp. nov. (A–G, J–M), Cothurnia buetschlii (J), and a case of fungus or developmental artefact (H, I, ZMB 12494), DIC. A. Segments 1–4, lateral and dorso-ventral view on the calyciform, dorso-ventrally compressed lorica with the zooid, ventrolateral view on basibiont. B. Epibiont with swarmer, stalk, and lorica in optical section. C. Epibiont with two macronuclei not fusing at any focal level. D, E. Young, small, spherical epibionts with few tentacles, not all tentacles in focus. F. Young specimen on cuticle of basibiont with striation pattern on surface (arrows). G. Epibiont with tentacles in empty shell of suctorian epibiont. H, I. Segment 10, dorsally (H) and optical section (I), subcuticular fungus or internal sclerotisation artefact of the cuticle (arrows). J. Assemblage of epibionts on segment 10 of basibiont with C. buetschlii (contracted specimen left, extended specimen right), T. husselae sp. nov., and two kinds of filamentous bacteria. Arrows mark endostyle. K. Suctorian on segment 7 of S. dentatus. L, M. Specimens collected 1998, showing cellular substructures (L) and withdrawn zooid with minimum amount of cellular substructures (M). Extended focus images of six (D), eight (H, L), and three (K) images. Arrowheads mark stalks in B, D, E, G, and J. Abbreviations: ba, bacteria; ca, basal cavity in lorica; co, C. buetschlii; en, endostyle; lo, lorica; ma, macronucleus; s, segment, followed by segment number; sc, spherical cavity; sp, sperm; st, stalk; su, suctorian epibiont; sw, swarmer; te, tentacle; tu, tube on segment 2. Scale bar in A 50 µm, in B 20 µm, valid for B, G–J and M, in C 20 µm, valid for C, K, and L, and in D–F 10 µm." figureDoi="http://doi.org/10.5281/zenodo.8349223" httpUri="https://zenodo.org/record/8349223/files/figure.png" pageId="9" pageNumber="448">Fig. 5M</figureCitation>
|
||
). This situation is interpreted as a fixation artefact. Few specimens were preserved more satisfyingly similar to the ones collected 2016, e. g., specimens on
|
||
<collectionCode id="18ECAE5B990AFF9AFD5EF95A7627F891" box="[762,823,1772,1798]" country="Germany" httpUri="http://grbio.org/cool/8syf-d21i" name="Museum für Naturkunde Berlin (Zoological Collections)" pageId="9" pageNumber="448" type="Museum">ZMB</collectionCode>
|
||
12397 and 12399 (
|
||
<figureCitation id="E6C62A1B990AFF9AFBB4F95D7176F891" box="[1040,1126,1771,1798]" captionStart="FIGURE 5" captionStartId="12.[151,250,186,210]" captionTargetId="figure-17@11.[151,1436,181,1725]" captionTargetPageId="11" captionText="FIGURE 5. Setaphyes kielensis (A, ZMB 12489; B, ZMB 12500; C, ZMB 12499; D, ZMB 12487; J, ZMB 12486; L, ZMB 12397; M, ZMB 12369) and S. dentatus (E–G, K, ZMB 12503; F, H, I, ZMB 12494) with specimens of Trematosoma husselae sp. nov. (A–G, J–M), Cothurnia buetschlii (J), and a case of fungus or developmental artefact (H, I, ZMB 12494), DIC. A. Segments 1–4, lateral and dorso-ventral view on the calyciform, dorso-ventrally compressed lorica with the zooid, ventrolateral view on basibiont. B. Epibiont with swarmer, stalk, and lorica in optical section. C. Epibiont with two macronuclei not fusing at any focal level. D, E. Young, small, spherical epibionts with few tentacles, not all tentacles in focus. F. Young specimen on cuticle of basibiont with striation pattern on surface (arrows). G. Epibiont with tentacles in empty shell of suctorian epibiont. H, I. Segment 10, dorsally (H) and optical section (I), subcuticular fungus or internal sclerotisation artefact of the cuticle (arrows). J. Assemblage of epibionts on segment 10 of basibiont with C. buetschlii (contracted specimen left, extended specimen right), T. husselae sp. nov., and two kinds of filamentous bacteria. Arrows mark endostyle. K. Suctorian on segment 7 of S. dentatus. L, M. Specimens collected 1998, showing cellular substructures (L) and withdrawn zooid with minimum amount of cellular substructures (M). Extended focus images of six (D), eight (H, L), and three (K) images. Arrowheads mark stalks in B, D, E, G, and J. Abbreviations: ba, bacteria; ca, basal cavity in lorica; co, C. buetschlii; en, endostyle; lo, lorica; ma, macronucleus; s, segment, followed by segment number; sc, spherical cavity; sp, sperm; st, stalk; su, suctorian epibiont; sw, swarmer; te, tentacle; tu, tube on segment 2. Scale bar in A 50 µm, in B 20 µm, valid for B, G–J and M, in C 20 µm, valid for C, K, and L, and in D–F 10 µm." figureDoi="http://doi.org/10.5281/zenodo.8349223" httpUri="https://zenodo.org/record/8349223/files/figure.png" pageId="9" pageNumber="448">Fig. 5L</figureCitation>
|
||
).
|
||
</paragraph>
|
||
</subSubSection>
|
||
</treatment>
|
||
</document> |