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<document ID-DOI="10.11646/phytotaxa.584.3.1" ID-ISSN="1179-3163" ID-Zenodo-Dep="7645598" approvalRequired="2" approvalRequired_for_textStreams="2" checkinTime="1676538281672" checkinUser="plazi" docAuthor="Ullah, Tauseef, Ullah, Khetab, Saba, Malka &amp; Shah, Fahim Hussain" docDate="2023" docId="03A5563FFF97297EFF7507D12228F93A" docLanguage="en" docName="phytotaxa.584.3.1.pdf" docOrigin="Phytotaxa 584 (3)" docSource="http://dx.doi.org/10.11646/phytotaxa.584.3.1" docStyle="DocumentStyle:96748F8F1B6C902996E134952A3A36B9.4:Phytotaxa.2014-.journal_article" docStyleId="96748F8F1B6C902996E134952A3A36B9" docStyleName="Phytotaxa.2014-.journal_article" docStyleVersion="4" docTitle="Conocybe karakensis T. Ullah, sp. nov." docType="treatment" docVersion="2" lastPageNumber="144" masterDocId="FF9C2E47FF902977FFFD04052650FFDC" masterDocTitle="Conocybe karakensis sp. nov. (Bolbitiaceae, Agaricales) from Pakistan" masterLastPageNumber="148" masterPageNumber="135" pageNumber="142" updateTime="1676538734682" updateUser="ExternalLinkService" zenodo-license-figures="UNSPECIFIED" zenodo-license-treatments="UNSPECIFIED">
<mods:mods xmlns:mods="http://www.loc.gov/mods/v3">
<mods:titleInfo>
<mods:title>Conocybe karakensis sp. nov. (Bolbitiaceae, Agaricales) from Pakistan</mods:title>
</mods:titleInfo>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Ullah, Tauseef</mods:namePart>
<mods:nameIdentifier type="ORCID">0000-0002-0420-9739</mods:nameIdentifier>
<mods:affiliation>Department of Plant Sciences, Quaid-i-Azam University, Islamabad, 45320, Pakistan &amp; tauseefullah 03 @ gmail. com; https: // orcid. org / 0000 - 0002 - 0420 - 9739</mods:affiliation>
<mods:nameIdentifier type="email">tauseefullah03@gmail.com</mods:nameIdentifier>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Ullah, Khetab</mods:namePart>
<mods:nameIdentifier type="ORCID">0000-0002-2143-9608</mods:nameIdentifier>
<mods:affiliation>Department of Plant Sciences, Quaid-i-Azam University, Islamabad, 45320, Pakistan &amp; khetabullah @ bs. qau. edu. pk; https: // orcid. org / 0000 - 0002 - 2143 - 9608</mods:affiliation>
<mods:nameIdentifier type="email">khetabullah@bs.qau.edu.pk</mods:nameIdentifier>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Saba, Malka</mods:namePart>
<mods:affiliation>Department of Plant Sciences, Quaid-i-Azam University, Islamabad, 45320, Pakistan</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Shah, Fahim Hussain</mods:namePart>
<mods:nameIdentifier type="ORCID">0000-0003-4851-8231</mods:nameIdentifier>
<mods:affiliation>Department of Plant Sciences, Quaid-i-Azam University, Islamabad, 45320, Pakistan &amp; fahimhussain 112233 @ gmail. com; https: // orcid. org / 0000 - 0003 - 4851 - 8231</mods:affiliation>
<mods:nameIdentifier type="email">fahimhussain112233@gmail.com</mods:nameIdentifier>
</mods:name>
<mods:typeOfResource>text</mods:typeOfResource>
<mods:relatedItem type="host">
<mods:titleInfo>
<mods:title>Phytotaxa</mods:title>
</mods:titleInfo>
<mods:part>
<mods:date>2023</mods:date>
<mods:detail type="pubDate">
<mods:number>2023-02-16</mods:number>
</mods:detail>
<mods:detail type="volume">
<mods:number>584</mods:number>
</mods:detail>
<mods:detail type="issue">
<mods:number>3</mods:number>
</mods:detail>
<mods:extent unit="page">
<mods:start>135</mods:start>
<mods:end>148</mods:end>
</mods:extent>
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<mods:location>
<mods:url>http://dx.doi.org/10.11646/phytotaxa.584.3.1</mods:url>
</mods:location>
<mods:classification>journal article</mods:classification>
<mods:identifier type="DOI">10.11646/phytotaxa.584.3.1</mods:identifier>
<mods:identifier type="ISSN">1179-3163</mods:identifier>
<mods:identifier type="Zenodo-Dep">7645598</mods:identifier>
</mods:mods>
<treatment LSID="urn:lsid:plazi:treatment:03A5563FFF97297EFF7507D12228F93A" httpUri="http://treatment.plazi.org/id/03A5563FFF97297EFF7507D12228F93A" lastPageId="9" lastPageNumber="144" pageId="7" pageNumber="142">
<subSubSection pageId="7" pageNumber="142" type="nomenclature">
<paragraph blockId="7.[136,1057,980,1007]" box="[136,1057,980,1007]" pageId="7" pageNumber="142">
<heading box="[136,1057,980,1007]" fontSize="11" level="2" pageId="7" pageNumber="142" reason="3">
<taxonomicName authority="T. Ullah" authorityName="T. Ullah" box="[136,479,980,1007]" class="Agaricomycetes" family="Bolbitiaceae" genus="Conocybe" kingdom="Fungi" order="Agaricales" pageId="7" pageNumber="142" phylum="Basidiomycota" rank="species" species="karakensis">
<emphasis bold="true" box="[136,381,980,1007]" italics="true" pageId="7" pageNumber="142">Conocybe karakensis</emphasis>
T. Ullah
</taxonomicName>
&amp; M.
<collectingCountry box="[554,608,980,1006]" name="Bonaire, Sint Eustatius and Saba" pageId="7" pageNumber="142">Saba</collectingCountry>
<taxonomicNameLabel box="[615,701,981,1007]" pageId="7" pageNumber="142" rank="species">
<emphasis box="[615,694,981,1006]" italics="true" pageId="7" pageNumber="142">sp. nov</emphasis>
.
</taxonomicNameLabel>
, (
<figureCitation box="[724,845,980,1006]" captionStart="FIGURE 2" captionStartId="8.[136,229,1988,2010]" captionTargetBox="[167,1421,190,1960]" captionTargetId="figure-23@8.[167,1421,190,1963]" captionTargetPageId="8" captionText="FIGURE 2. AE. Macro-morphological characterizations of Conocybe karakensis sp. nov. A &amp; E. Photography click at their natural ecological habitat, B &amp; C. Gills and stipe view, D. Upper view of basidiomata. Scale bar: AB = 5 mm. Photos click by Tauseef Ullah." figureDoi="http://doi.org/10.5281/zenodo.7645602" httpUri="https://zenodo.org/record/7645602/files/figure.png" pageId="7" pageNumber="142">Fig. 2AE</figureCitation>
,
<figureCitation box="[856,924,980,1006]" captionStart="FIGURE 3" captionStartId="9.[136,229,1600,1622]" captionTargetBox="[327,1261,192,1565]" captionTargetId="figure-18@9.[327,1261,190,1576]" captionTargetPageId="9" captionText="FIGURE 3. ab. Scanning electron microscope (SEM) analysis of Basidiospores. (source: National Center of Excellence in Geology, University of Peshawar, Pakistan)." figureDoi="http://doi.org/10.5281/zenodo.7645604" httpUri="https://zenodo.org/record/7645604/files/figure.png" pageId="7" pageNumber="142">3. ab</figureCitation>
and
<figureCitation box="[979,1048,980,1006]" captionStart="FIGURE 4" captionStartId="10.[136,229,2008,2030]" captionTargetBox="[202,1360,257,1953]" captionTargetId="figure-52@10.[199,1389,245,1984]" captionTargetPageId="10" captionText="FIGURE 4.AG. Micro-morphological features of Conocybe karakensis sp. nov. A. Basidiospores under light microscope, B. Basidia, C. Cheilocystidia, D. Pleurocystidia, E &amp; F. Pileipellis materials, G. Stipitipellis meterials. Bars = 10μm" figureDoi="http://doi.org/10.5281/zenodo.7645606" httpUri="https://zenodo.org/record/7645606/files/figure.png" pageId="7" pageNumber="142">4AG</figureCitation>
)
</heading>
</paragraph>
<paragraph blockId="7.[136,371,1020,1042]" box="[136,371,1020,1042]" pageId="7" pageNumber="142">MycoBank: MB 843865</paragraph>
</subSubSection>
<subSubSection lastPageId="9" lastPageNumber="144" pageId="7" pageNumber="142" type="etymology">
<paragraph blockId="7.[136,1452,1088,2086]" box="[136,1280,1088,1115]" pageId="7" pageNumber="142">
Etymology:—karakensis refers to the locality Karak District where the
<typeStatus box="[953,1001,1089,1115]" pageId="7" pageNumber="142">type</typeStatus>
specimen was collected.
</paragraph>
<paragraph blockId="7.[136,1452,1088,2086]" pageId="7" pageNumber="142">
Diagnosis:—The diagnostic features of
<emphasis box="[645,983,1124,1150]" italics="true" pageId="7" pageNumber="142">
<taxonomicName authority="Ullah &amp; Ullah &amp; Saba &amp; Shah, 2023" authorityName="Ullah &amp; Ullah &amp; Saba &amp; Shah" authorityYear="2023" box="[645,887,1124,1150]" class="Agaricomycetes" family="Bolbitiaceae" genus="Conocybe" kingdom="Fungi" order="Agaricales" pageId="7" pageNumber="142" phylum="Basidiomycota" rank="species" species="karakensis" status="sp. nov.">Conocybe karakensis</taxonomicName>
<taxonomicNameLabel box="[896,983,1125,1150]" pageId="7" pageNumber="142" rank="species">sp. nov.</taxonomicNameLabel>
</emphasis>
are; medium size cap, semi-ovate pileus with deep reddish-orange (10R 4/12 #AF3615) to dark reddish-orange (10R 4/10 #A73F27) color; light brown, large sized stipe; thick-walled, light brown, oblong basidiospores; clavate shaped basidia; inhabiting in the tropical area (mixture of sand and loam soil).
</paragraph>
<paragraph blockId="7.[136,1452,1088,2086]" pageId="7" pageNumber="142">
<materialsCitation collectingDate="2017-03-04" country="Pakistan" elevation="340" location="Karak District" longitude="32.161392" pageId="7" pageNumber="142" specimenCount="1" stateProvince="Khyber Pakhtunkhwa" typeStatus="holotype">
<typeStatus box="[189,246,1268,1294]" pageId="7" pageNumber="142">Type</typeStatus>
:—
<collectingCountry box="[278,418,1268,1294]" name="Pakistan" pageId="7" pageNumber="142">PAKISTAN</collectingCountry>
.
<collectingRegion box="[429,784,1268,1294]" country="Pakistan" name="Khyber Pakhtunkhwa" pageId="7" pageNumber="142">Khyber Pakhtunkhwa Province</collectingRegion>
:
<location LSID="urn:lsid:plazi:treatment:03A5563FFF97297EFF7507D12228F93A:8ED3B1F2FF972970FCE600F125EBFAD2" box="[795,955,1268,1294]" country="Pakistan" longitude="32.161392" name="Karak District" pageId="7" pageNumber="142" stateProvince="Khyber Pakhtunkhwa">Karak District</location>
(Mianki banda), N71º7ʹ67ʺ
<geoCoordinate box="[1273,1388,1269,1296]" degrees="32" direction="east" minutes="9" orientation="longitude" pageId="7" pageNumber="142" precision="15" seconds="41" value="32.161392">E32º9ʹ41ʺ</geoCoordinate>
, elev.
<quantity box="[136,209,1304,1331]" metricMagnitude="2" metricUnit="m" metricValue="3.4" pageId="7" pageNumber="142" unit="m" value="340.0">
<elevation box="[136,209,1304,1331]" metricMagnitude="2" metricUnit="m" metricValue="3.4" pageId="7" pageNumber="142" unit="m" value="340.0">340 m</elevation>
</quantity>
, in sandy and loamy soil,
<date box="[501,661,1304,1330]" pageId="7" pageNumber="142" value="2017-03-04">
<collectingDate box="[501,661,1304,1330]" pageId="7" pageNumber="142" value="2017-03-04">4 March 2017</collectingDate>
</date>
, Tauseef Ullah (KTK-05)
</materialsCitation>
.
</paragraph>
<paragraph blockId="7.[136,1452,1088,2086]" pageId="7" pageNumber="142">
Description:—Small to medium size fruiting bodies having no prominent odor.
<emphasis box="[1082,1152,1340,1366]" italics="true" pageId="7" pageNumber="142">Pileus</emphasis>
<quantity box="[1159,1242,1340,1367]" metricMagnitude="-2" metricUnit="m" metricValue="5.5" metricValueMax="6.0" metricValueMin="5.0" pageId="7" pageNumber="142" unit="cm" value="5.5" valueMax="6.0" valueMin="5.0">56 cm</quantity>
broad and
<quantity metricMagnitude="-3" metricUnit="m" metricValue="3.5" metricValueMax="5.0" metricValueMin="2.0" pageId="7" pageNumber="142" unit="cm" value="0.35" valueMax="0.5" valueMin="0.2">0.20.5 cm</quantity>
thick, smooth, semi-ovate, fleshy at the initial stage but later on become collapsed and auto-degraded at maturity, epigeous, no discoloration on touching, lack of fibrillose scale on surface, striate margin, deep reddish orange in color (10R 4/12 #AF3615) at disk while turning dark reddish orange (10R 4/10 #A73F27) toward the margin.
<emphasis box="[1299,1403,1448,1474]" italics="true" pageId="7" pageNumber="142">Lamellae</emphasis>
<quantity metricMagnitude="-2" metricUnit="m" metricValue="2.5" metricValueMax="3.0" metricValueMin="2.0" pageId="7" pageNumber="142" unit="cm" value="2.5" valueMax="3.0" valueMin="2.0">23 cm</quantity>
broad, dark reddish-orange in color, free, spaced with intercalated lemellulae of one tire.
<emphasis box="[1189,1245,1484,1510]" italics="true" pageId="7" pageNumber="142">Stipe</emphasis>
ranging from
<quantity metricMagnitude="-2" metricUnit="m" metricValue="7.0" metricValueMax="8.0" metricValueMin="6.0" pageId="7" pageNumber="142" unit="cm" value="7.0" valueMax="8.0" valueMin="6.0">68 cm</quantity>
long and
<quantity box="[281,374,1520,1547]" metricMagnitude="-3" metricUnit="m" metricValue="4.0" metricValueMax="5.0" metricValueMin="3.0" pageId="7" pageNumber="142" unit="mm" value="4.0" valueMax="5.0" valueMin="3.0">35 mm</quantity>
thick, entirely smooth, cylindrical, woody, solid, central, and deep reddish orange to dark radish orange in color.
</paragraph>
<paragraph blockId="7.[136,1452,1088,2086]" pageId="7" pageNumber="142">
<emphasis box="[189,347,1592,1618]" italics="true" pageId="7" pageNumber="142">Basidiospores</emphasis>
11.815 × 7.29.7 μm, elongated, oblong-shaped, smooth and thick-walled having prominent black colour, non-amyloid, apiculus not observed, oil globule absent, light brown in 5% KOH, dark brown in
<collectingCountry box="[1323,1398,1628,1654]" name="Democratic Republic of the Congo" pageId="7" pageNumber="142">Congo</collectingCountry>
Red while light greenish in lactophenol.
<emphasis box="[529,615,1664,1690]" italics="true" pageId="7" pageNumber="142">Basidia</emphasis>
14.825 × 1013.5 μm, bisterigmatic or tetrasterigmatic bearing two or four spores, sharped sterigmata (2.35.4 μm), nearly ellipsoid-obovoid shaped, rounded base, thin-walled, non-amyloid, oil globules absent.
<emphasis box="[359,521,1736,1762]" italics="true" pageId="7" pageNumber="142">Cheilocystidia</emphasis>
22.338.5 × 6.411.8 μm, clavate shaped, light brown in color.
<emphasis box="[1233,1395,1736,1762]" italics="true" pageId="7" pageNumber="142">Pleurocystidia</emphasis>
12.2 23.6 × 8.211.4 µm, smooth and thin-walled, slightly depressed at centre, broadly clavate shaped, light brown in KOH, somewhat greenish in lactophenol while dark brown in
<collectingCountry box="[834,909,1808,1834]" name="Democratic Republic of the Congo" pageId="7" pageNumber="142">Congo</collectingCountry>
Red dye and rare in number. Pileipellis material 5.220.4 μm, smooth and thick-walled, oblong, sub-spherical to spherical shaped. Hymenophoral trama irregular, composed of thin-walled hyphae ranging from 10.522.6 broad.
<emphasis box="[867,995,1880,1906]" italics="true" pageId="7" pageNumber="142">Stipitipellis</emphasis>
18.328.3 μm broad, cylindrical, brown in color, regular, septate, unbranched, clamp connection absent.
</paragraph>
<paragraph blockId="7.[136,1452,1088,2086]" pageId="7" pageNumber="142">
Habit and habitat:—This species was found solitary in a tropical habitat during the spring season (March) on loam soil mixed with sand soil surrounded by
<emphasis box="[591,794,1988,2014]" italics="true" pageId="7" pageNumber="142">Cynodon dectylon</emphasis>
(L.) Pers and
<emphasis box="[953,1159,1989,2014]" italics="true" pageId="7" pageNumber="142">Treticum aestivum</emphasis>
(L.).
</paragraph>
<paragraph blockId="7.[136,1452,1088,2086]" box="[189,928,2024,2050]" pageId="7" pageNumber="142">
Distribution:—Only known from
<collectingRegion box="[568,814,2024,2050]" country="Pakistan" name="Khyber Pakhtunkhwa" pageId="7" pageNumber="142">Khyber Pakhtunkhwa</collectingRegion>
,
<collectingCountry box="[826,925,2024,2050]" name="Pakistan" pageId="7" pageNumber="142">Pakistan</collectingCountry>
.
</paragraph>
<paragraph blockId="7.[136,1452,1088,2086]" box="[189,441,2060,2086]" pageId="7" pageNumber="142">Edibility:—Unknown.</paragraph>
<caption ID-DOI="http://doi.org/10.5281/zenodo.7645602" ID-Zenodo-Dep="7645602" httpUri="https://zenodo.org/record/7645602/files/figure.png" pageId="8" pageNumber="143" startId="8.[136,229,1988,2010]" targetBox="[167,1421,190,1960]" targetPageId="8">
<paragraph blockId="8.[136,1452,1988,2046]" pageId="8" pageNumber="143">
<emphasis bold="true" box="[136,314,1988,2010]" pageId="8" pageNumber="143">FIGURE 2. AE.</emphasis>
Macro-morphological characterizations of
<emphasis box="[741,1032,1988,2009]" italics="true" pageId="8" pageNumber="143">
<taxonomicName authority="Ullah &amp; Ullah &amp; Saba &amp; Shah, 2023" authorityName="Ullah &amp; Ullah &amp; Saba &amp; Shah" authorityYear="2023" box="[741,948,1988,2009]" class="Agaricomycetes" family="Bolbitiaceae" genus="Conocybe" kingdom="Fungi" order="Agaricales" pageId="8" pageNumber="143" phylum="Basidiomycota" rank="species" species="karakensis" status="sp. nov.">Conocybe karakensis</taxonomicName>
<taxonomicNameLabel box="[956,1032,1988,2009]" pageId="8" pageNumber="143" rank="species">sp. nov.</taxonomicNameLabel>
</emphasis>
<emphasis bold="true" box="[1038,1113,1988,2010]" pageId="8" pageNumber="143">A &amp; E.</emphasis>
Photography click at their natural ecological habitat,
<emphasis bold="true" box="[318,389,2024,2046]" pageId="8" pageNumber="143">B &amp; C.</emphasis>
Gills and stipe view,
<emphasis bold="true" box="[597,621,2024,2045]" pageId="8" pageNumber="143">D.</emphasis>
Upper view of basidiomata. Scale bar: AB = 5 mm. Photos click by Tauseef Ullah.
</paragraph>
</caption>
<caption ID-DOI="http://doi.org/10.5281/zenodo.7645604" ID-Zenodo-Dep="7645604" httpUri="https://zenodo.org/record/7645604/files/figure.png" pageId="9" pageNumber="144" startId="9.[136,229,1600,1622]" targetBox="[327,1261,192,1565]" targetPageId="9">
<paragraph blockId="9.[136,1452,1600,1658]" pageId="9" pageNumber="144">
<emphasis bold="true" box="[136,306,1600,1622]" pageId="9" pageNumber="144">FIGURE 3. ab.</emphasis>
Scanning electron microscope (SEM) analysis of Basidiospores. (source: National Center of Excellence in Geology, University of Peshawar, Pakistan).
</paragraph>
</caption>
<paragraph blockId="9.[136,1451,1704,1769]" box="[189,1451,1704,1730]" pageId="9" pageNumber="144">
Other specimen examined:—
<collectingCountry box="[531,671,1704,1730]" name="Pakistan" pageId="9" pageNumber="144">PAKISTAN</collectingCountry>
.
<collectingRegion box="[690,1061,1704,1730]" country="Pakistan" name="Khyber Pakhtunkhwa" pageId="9" pageNumber="144">Khyber Pakhtunkhwa Province</collectingRegion>
: District Karak, Takht-e-Nasrati,
</paragraph>
<paragraph blockId="9.[136,1451,1704,1769]" box="[136,1144,1740,1769]" pageId="9" pageNumber="144">
N71º7ʹ67ʺ
<geoCoordinate box="[258,371,1742,1769]" degrees="32" direction="east" minutes="9" orientation="longitude" pageId="9" pageNumber="144" precision="15" seconds="41" value="32.161392">E32º9ʹ41ʺ</geoCoordinate>
elev.
<quantity box="[436,509,1741,1767]" metricMagnitude="2" metricUnit="m" metricValue="3.4" pageId="9" pageNumber="144" unit="m" value="340.0">340 m</quantity>
, in wheat field,
<date box="[689,849,1740,1767]" pageId="9" pageNumber="144" value="2018-03-09">9 March 2018</date>
, Tauseef Ullah (KTK-06).
</paragraph>
</subSubSection>
</treatment>
</document>