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<document ID-DOI="10.11646/phytotaxa.578.2.5" ID-ISSN="1179-3163" ID-Zenodo-Dep="7517860" checkinTime="1673306621596" checkinUser="plazi" docAuthor="Haqnawaz, Muhammad, Khan, Zaman, Niazi, Abdul Rehman &amp; Khalid, Abdul Nasir" docDate="2023" docId="039787DFFF84FFBEFF6CBF1091EDFC25" docLanguage="en" docName="phytotaxa.578.2.5.pdf" docOrigin="Phytotaxa 578 (2)" docSource="http://dx.doi.org/10.11646/phytotaxa.578.2.5" docStyle="DocumentStyle:96748F8F1B6C902996E134952A3A36B9.5:Phytotaxa.2014-.journal_article" docStyleId="96748F8F1B6C902996E134952A3A36B9" docStyleName="Phytotaxa.2014-.journal_article" docStyleVersion="5" docTitle="Volvariella variicystidiosa Haqnawaz, Zaman, Niazi &amp; Khalid 2023, sp. nov." docType="treatment" docVersion="1" lastPageNumber="191" masterDocId="FFAEFFA7FF86FFBCFFE4BC079372F43F" masterDocTitle="Volvariella variicystidiosa sp. nov. (Agaricaceae, Basidiomycota) from Punjab, Pakistan" masterLastPageNumber="198" masterPageNumber="189" pageNumber="191" updateTime="1673607966765" updateUser="tatiana" zenodo-license-figures="UNSPECIFIED" zenodo-license-treatments="UNSPECIFIED">
<mods:mods xmlns:mods="http://www.loc.gov/mods/v3">
<mods:titleInfo>
<mods:title>Volvariella variicystidiosa sp. nov. (Agaricaceae, Basidiomycota) from Punjab, Pakistan</mods:title>
</mods:titleInfo>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Haqnawaz, Muhammad</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Khan, Zaman</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Niazi, Abdul Rehman</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Khalid, Abdul Nasir</mods:namePart>
</mods:name>
<mods:typeOfResource>text</mods:typeOfResource>
<mods:relatedItem type="host">
<mods:titleInfo>
<mods:title>Phytotaxa</mods:title>
</mods:titleInfo>
<mods:part>
<mods:date>2023</mods:date>
<mods:detail type="pubDate">
<mods:number>2023-01-06</mods:number>
</mods:detail>
<mods:detail type="volume">
<mods:number>578</mods:number>
</mods:detail>
<mods:detail type="issue">
<mods:number>2</mods:number>
</mods:detail>
<mods:extent unit="page">
<mods:start>189</mods:start>
<mods:end>198</mods:end>
</mods:extent>
</mods:part>
</mods:relatedItem>
<mods:location>
<mods:url>http://dx.doi.org/10.11646/phytotaxa.578.2.5</mods:url>
</mods:location>
<mods:classification>journal article</mods:classification>
<mods:identifier type="DOI">10.11646/phytotaxa.578.2.5</mods:identifier>
<mods:identifier type="ISSN">1179-3163</mods:identifier>
<mods:identifier type="Zenodo-Dep">7517860</mods:identifier>
</mods:mods>
<treatment LSID="urn:lsid:plazi:treatment:039787DFFF84FFBEFF6CBF1091EDFC25" httpUri="http://treatment.plazi.org/id/039787DFFF84FFBEFF6CBF1091EDFC25" lastPageNumber="191" pageId="2" pageNumber="191">
<subSubSection box="[136,963,791,814]" pageId="2" pageNumber="191" type="nomenclature">
<paragraph blockId="2.[136,1452,791,1102]" box="[136,963,791,814]" pageId="2" pageNumber="191">
<taxonomicName authority="Haqnawaz, Zaman, Niazi &amp; Khalid" authorityName="Haqnawaz, Zaman, Niazi &amp; Khalid" authorityYear="2023" box="[136,739,791,814]" class="Agaricomycetes" family="Pluteaceae" genus="Volvariella" kingdom="Fungi" order="Agaricales" pageId="2" pageNumber="191" phylum="Basidiomycota" rank="species" species="variicystidiosa" status="sp. nov.">
<emphasis bold="true" box="[136,390,791,813]" italics="true" pageId="2" pageNumber="191">Volvariella variicystidiosa</emphasis>
Haqnawaz, Zaman, Niazi &amp; Khalid
</taxonomicName>
,
<emphasis box="[748,821,792,813]" italics="true" pageId="2" pageNumber="191">
<taxonomicNameLabel box="[748,821,792,813]" pageId="2" pageNumber="191" rank="species">sp. nov.</taxonomicNameLabel>
</emphasis>
(
<figureCitation box="[833,924,792,814]" captionStart="FIGURE 1" captionStartId="3.[136,229,1518,1540]" captionTargetBox="[152,1436,190,1494]" captionTargetId="figure-18@3.[151,1436,190,1494]" captionTargetPageId="3" captionText="FIGURE 1. AD. Fruitbodies of Volvariella variicystidiosa sp. nov. (A. holotype LAH37568). Scale bars: AD = 05 mm. Photos by: Muhammad Haqnawaz &amp; Zaman Khan." figureDoi="http://doi.org/10.5281/zenodo.7517862" httpUri="https://zenodo.org/record/7517862/files/figure.png" pageId="2" pageNumber="191">Figures 1</figureCitation>
,
<figureCitation box="[936,950,792,813]" captionStart="FIGURE 2" captionStartId="4.[136,229,1971,1993]" captionTargetBox="[242,1346,317,1947]" captionTargetId="figure-88@4.[242,1346,317,1947]" captionTargetPageId="4" captionText="FIGURE 2. AF. Microscopical structures of V. variicystidiosa (Holotype) A. Basidia, B. Basidiospores, C. Pleurocystidia, D. Cheilocystidia, E. Pileus covering Hyphae F. Stipe covering Hyphae, Scale bars: AC = 5 µm, D = 10 µm, EF = 25 µm.All elements in Congo Red. Photos by: Muhammad Haqnawaz." figureDoi="http://doi.org/10.5281/zenodo.7517864" httpUri="https://zenodo.org/record/7517864/files/figure.png" pageId="2" pageNumber="191">2</figureCitation>
).
</paragraph>
</subSubSection>
<subSubSection box="[136,321,828,850]" pageId="2" pageNumber="191" type="description">
<paragraph blockId="2.[136,1452,791,1102]" box="[136,321,828,850]" pageId="2" pageNumber="191">MycoBank 845960</paragraph>
</subSubSection>
<subSubSection box="[136,1214,864,886]" pageId="2" pageNumber="191" type="etymology">
<paragraph blockId="2.[136,1452,791,1102]" box="[136,1214,864,886]" pageId="2" pageNumber="191">
Etymology:—The specific epithet “
<taxonomicName authority="(Latin)" authorityName="Haqnawaz, Zaman, Niazi &amp; Khalid" authorityYear="2023" baseAuthorityName="Latin" box="[485,626,864,885]" class="Agaricomycetes" family="Pluteaceae" genus="Volvariella" kingdom="Fungi" order="Agaricales" pageId="2" pageNumber="191" phylum="Basidiomycota" rank="species" species="variicystidiosa">
<emphasis box="[485,626,864,885]" italics="true" pageId="2" pageNumber="191">variicystidiosa</emphasis>
</taxonomicName>
” (Latin) refers to variable shapes and sizes of cheilocystidia.
</paragraph>
</subSubSection>
<subSubSection pageId="2" pageNumber="191" type="diagnosis">
<paragraph blockId="2.[136,1452,791,1102]" pageId="2" pageNumber="191">Diagnosis:—convex to concave pileus, dull brown umbo with grayish brown striations; basidiospores broadly ellipsoid to obovoid; cheilocystidia abundant variable in shape, cymbiform to mucronate, narrowly to broadly moniliform with a broad base, broadly ellipsoid, triangular to conical; pleurocystidia moderately ellipsoid, rarely flexuous, and oblong; light yellow to gray and terminal cell broad cylindrical, apically often mucronate.</paragraph>
</subSubSection>
<subSubSection pageId="2" pageNumber="191" type="materials_examined">
<paragraph blockId="2.[136,1452,791,1102]" pageId="2" pageNumber="191">
<materialsCitation accessionNumber="OP549026" collectingDate="2020-09-03" country="Pakistan" county="Muzaffargarh district" elevation="134" latitude="30.245556" location="Kot Addu" longLatPrecision="20" longitude="70.85111" municipality="Indus Riverbed" pageId="2" pageNumber="191" specimenCount="1" stateProvince="Punjab" typeStatus="holotype">
<typeStatus box="[136,225,1044,1066]" pageId="2" pageNumber="191">Holotype</typeStatus>
:—
<collectingCountry box="[253,374,1044,1066]" name="Pakistan" pageId="2" pageNumber="191">PAKISTAN</collectingCountry>
.
<collectingRegion box="[382,451,1044,1066]" country="Pakistan" name="Punjab" pageId="2" pageNumber="191">Punjab</collectingRegion>
,
<collectingCounty box="[460,666,1044,1066]" pageId="2" pageNumber="191">Muzaffargarh district</collectingCounty>
,
<collectingMunicipality box="[675,824,1044,1066]" pageId="2" pageNumber="191">Indus Riverbed</collectingMunicipality>
,
<location LSID="urn:lsid:plazi:treatment:039787DFFF84FFBEFF6CBF1091EDFC25:8EE16012FF84FFBEFCA6B81390D0F015" box="[834,930,1044,1066]" country="Pakistan" county="Muzaffargarh district" latitude="30.245556" longLatPrecision="20" longitude="70.85111" municipality="Indus Riverbed" name="Kot Addu" pageId="2" pageNumber="191" stateProvince="Punjab">Kot Addu</location>
, (
<geoCoordinate box="[946,1072,1044,1065]" degrees="30" direction="north" minutes="14" orientation="latitude" pageId="2" pageNumber="191" precision="15" seconds="44" value="30.245556">30°1444” N</geoCoordinate>
,
<geoCoordinate box="[1082,1205,1044,1066]" degrees="70" direction="east" minutes="51" orientation="longitude" pageId="2" pageNumber="191" precision="15" seconds="04" value="70.85111">70°5104” E</geoCoordinate>
,
<elevation box="[1214,1324,1044,1066]" metricMagnitude="2" metricUnit="m" metricValue="1.34" pageId="2" pageNumber="191" unit="m" value="134.0">
<quantity box="[1214,1275,1044,1065]" metricMagnitude="2" metricUnit="m" metricValue="1.34" pageId="2" pageNumber="191" unit="m" value="134.0">134 m</quantity>
a.s.l.
</elevation>
) on soil, rich in organic matter,
<date box="[364,559,1080,1102]" pageId="2" pageNumber="191" value="2020-09-03">
<collectingDate box="[364,559,1080,1102]" pageId="2" pageNumber="191" value="2020-09-03">September 03, 2020</collectingDate>
</date>
,
<emphasis box="[569,792,1080,1101]" italics="true" pageId="2" pageNumber="191">Muhammad Haqnawaz</emphasis>
(LAH37568). GenBank
<accessionNumber box="[1032,1134,1080,1102]" httpUri="https://www.ebi.ac.uk/ena/browser/api/embl/OP549026" pageId="2" pageNumber="191">OP549026</accessionNumber>
(ITS).
</materialsCitation>
</paragraph>
</subSubSection>
<subSubSection pageId="2" pageNumber="191" type="description">
<paragraph blockId="2.[136,1452,1148,2075]" pageId="2" pageNumber="191">
Description:—
<emphasis box="[302,444,1148,1174]" italics="true" pageId="2" pageNumber="191">Basidiomata</emphasis>
medium-sized.
<emphasis box="[622,692,1148,1174]" italics="true" pageId="2" pageNumber="191">Pileus</emphasis>
<quantity box="[698,820,1148,1175]" metricMagnitude="-2" metricUnit="m" metricValue="1.95" metricValueMax="2.4" metricValueMin="1.5" pageId="2" pageNumber="191" unit="mm" value="19.5" valueMax="24.0" valueMin="15.0">1524 mm</quantity>
, campanulate when young becoming convex to concave, umbonate at maturity, serrate margins, split like grayish brown (5 YR 6/2) striations toward margin on light gray (5 YR 6/2) surface, light gray (10YR 7/1) to dull brown (7.5 YR 5/3) umbo at the mature stage, wrinkled, sulcate to slightly split cap margin, soft, dry, thin and light gray (10YR 7/1) context.
<emphasis box="[912,1016,1256,1282]" italics="true" pageId="2" pageNumber="191">Lamellae</emphasis>
dull orange (5 YR 7/3), free, narrow, medium size,
<quantity box="[291,383,1292,1319]" metricMagnitude="-3" metricUnit="m" metricValue="3.0" metricValueMax="4.0" metricValueMin="2.0" pageId="2" pageNumber="191" unit="mm" value="3.0" valueMax="4.0" valueMin="2.0">24 mm</quantity>
width, average, crowded, fimbriate edges, arid misc, branched, 12 tiers.
<emphasis box="[1204,1260,1292,1318]" italics="true" pageId="2" pageNumber="191">Stipe</emphasis>
48
<quantity box="[1310,1389,1292,1319]" metricMagnitude="-2" metricUnit="m" metricValue="2.5" pageId="2" pageNumber="191" unit="mm" value="25.0">25 mm</quantity>
, light gray (7.5 Y 8/1), central, flexuous, very slightly thickened downward, rounded, rough surface, hairy, mycelium present on the base,
<emphasis box="[273,335,1364,1390]" italics="true" pageId="2" pageNumber="191">Volva</emphasis>
dark grayish yellow (2.5 Y 4/2) color with three to four limbs,
<quantity box="[1030,1122,1364,1391]" metricMagnitude="-3" metricUnit="m" metricValue="4.0" metricValueMax="5.0" metricValueMin="3.0" pageId="2" pageNumber="191" unit="mm" value="4.0" valueMax="5.0" valueMin="3.0">35 mm</quantity>
length.
<emphasis box="[1211,1303,1364,1390]" italics="true" pageId="2" pageNumber="191">Annulus</emphasis>
absent.
<emphasis italics="true" pageId="2" pageNumber="191">Taste and odor</emphasis>
were not recorded.
</paragraph>
<paragraph blockId="2.[136,1452,1148,2075]" pageId="2" pageNumber="191">
<emphasis box="[189,347,1436,1462]" italics="true" pageId="2" pageNumber="191">Basidiospores</emphasis>
[60/2/2] (5.3)5.8 7.4(7.6) × (3.7) 4.1 5.1(5.5) µm, avl × avw = 6.5 × 4.7 um, Q = 1.131.62 µm, Qav = 1.37 µm, broadly ellipsoid to obovoid, hyaline to light brown in 5% KOH, thick-walled, guttulate.
<emphasis box="[1366,1452,1472,1498]" italics="true" pageId="2" pageNumber="191">Basidia</emphasis>
2433 × 7710 µm, hyaline in 5% KOH, 24 spored, thick-walled, clavate, fusiform, smooth, guttulate, clamp connection absent.
<emphasis box="[358,520,1544,1570]" italics="true" pageId="2" pageNumber="191">Cheilocystidia</emphasis>
2492 × 1037 µm, abundant, broad variation in size and shape, cymbiform to mucronate, narrowly to broadly moniliform with a broad base, and moderately knobbed lageniform, narrowly to broadly fusiform, utriform, rarely broadly ellipsoid to ellipsoid with obtuse apex, triangular to conical hyaline in 5% KOH, and clamp connection absent.
<emphasis box="[604,766,1652,1678]" italics="true" pageId="2" pageNumber="191">Pleurocystidia</emphasis>
4281 × 1228 µm, extremely variable in size and shape, moderately narrowly utriform, broadly clavate, ellipsoid, fusiform, rarely flexuous, oblong, hyaline in 5% KOH, and clamp connection absent.
<emphasis box="[435,549,1724,1750]" italics="true" pageId="2" pageNumber="191">Pileipellis</emphasis>
1335 µm diam., cutis
<typeStatus box="[867,918,1725,1751]" pageId="2" pageNumber="191">type</typeStatus>
, terminal cell broad cylindrical, apically often mucronate, 72136 × 1230 µm, light yellow to gray in 5% KOH, smooth, branched, septate and clamp connections absent.
<emphasis box="[224,352,1796,1822]" italics="true" pageId="2" pageNumber="191">Stipitipellis</emphasis>
925 µm diam., thin-walled, irregular intricate cutis
<typeStatus box="[1004,1055,1797,1823]" pageId="2" pageNumber="191">type</typeStatus>
, light yellow to gray in 5% KOH, branched, clamp connections absent. rarely clavate and smooth caulocystidia present.
</paragraph>
</subSubSection>
<subSubSection box="[189,941,1868,1895]" pageId="2" pageNumber="191" type="biology_ecology">
<paragraph blockId="2.[136,1452,1148,2075]" box="[189,941,1868,1895]" pageId="2" pageNumber="191">
<emphasis bold="true" box="[189,284,1868,1894]" pageId="2" pageNumber="191">Ecology</emphasis>
: Scattered, terrestrial, on sandy soil rich in organic matter.
</paragraph>
</subSubSection>
<subSubSection pageId="2" pageNumber="191" type="materials_examined">
<paragraph blockId="2.[136,1452,1148,2075]" pageId="2" pageNumber="191">
<emphasis bold="true" box="[189,564,1904,1930]" pageId="2" pageNumber="191">Additional materials examined</emphasis>
:
<materialsCitation accessionNumber="OP549027, OP549028, OP549025" collectingDate="2019-08-16" collectingDateMax="2021-08-08" collectingDateMin="2019-08-16" country="Pakistan" county="Indus River" elevation="134" latitude="31.988155" location="Muzaffargarh" longLatPrecision="1" longitude="71.99052" municipality="Kot Addu" pageId="2" pageNumber="191" specimenCount="1" stateProvince="Punjab">
<collectingCountry box="[583,681,1904,1930]" name="Pakistan" pageId="2" pageNumber="191">Pakistan</collectingCountry>
,
<collectingRegion box="[695,776,1904,1930]" country="Pakistan" name="Punjab" pageId="2" pageNumber="191">Punjab</collectingRegion>
, bed of
<collectingCounty box="[874,1010,1904,1930]" pageId="2" pageNumber="191">Indus River</collectingCounty>
,
<collectingMunicipality box="[1025,1141,1904,1931]" pageId="2" pageNumber="191">Kot Addu</collectingMunicipality>
,
<location LSID="urn:lsid:plazi:treatment:039787DFFF84FFBEFF6CBF1091EDFC25:8EE16012FF84FFBEFB67BB77966DF3B5" box="[1155,1311,1904,1930]" country="Pakistan" county="Indus River" latitude="31.988155" longLatPrecision="1" longitude="71.99052" municipality="Kot Addu" name="Muzaffargarh" pageId="2" pageNumber="191" stateProvince="Punjab">Muzaffargarh</location>
, (
<geoCoordinate degrees="30" direction="north" minutes="14" orientation="latitude" pageId="2" pageNumber="191" precision="15" seconds="44" value="30.245556">30°1444” N</geoCoordinate>
,
<geoCoordinate box="[172,316,1940,1967]" degrees="70" direction="east" minutes="51" orientation="longitude" pageId="2" pageNumber="191" precision="15" seconds="04" value="70.85111">70°5104” E</geoCoordinate>
,
<elevation box="[330,461,1940,1967]" metricMagnitude="2" metricUnit="m" metricValue="1.34" pageId="2" pageNumber="191" unit="m" value="134.0">
<quantity box="[330,402,1940,1967]" metricMagnitude="2" metricUnit="m" metricValue="1.34" pageId="2" pageNumber="191" unit="m" value="134.0">134 m</quantity>
a.s.l.
</elevation>
) on soil rich in organic matter,
<date box="[824,1015,1940,1966]" pageId="2" pageNumber="191" value="2019-08-16">
<collectingDate box="[824,1015,1940,1966]" pageId="2" pageNumber="191" value="2019-08-16">August 16, 2019</collectingDate>
</date>
,
<emphasis box="[1028,1290,1940,1966]" italics="true" pageId="2" pageNumber="191">
<location LSID="urn:lsid:plazi:treatment:039787DFFF84FFBEFF6CBF1091EDFC25:8EE16012FF84FFBEFBE0BB939678F391" box="[1028,1290,1940,1966]" country="Pakistan" county="Indus River" latitude="31.988155" longLatPrecision="1" longitude="71.99052" municipality="Kot Addu" name="Muhammad Haqnawaz" pageId="2" pageNumber="191" stateProvince="Punjab">Muhammad Haqnawaz</location>
</emphasis>
(LAH37569).
<location LSID="urn:lsid:plazi:treatment:039787DFFF84FFBEFF6CBF1091EDFC25:8EE16012FF84FFBEFF6CBBBF9380F3ED" box="[136,242,1976,2002]" country="Pakistan" county="Indus River" latitude="31.988155" longLatPrecision="1" longitude="71.99052" municipality="Kot Addu" name="GenBank" pageId="2" pageNumber="191" stateProvince="Punjab">GenBank</location>
<accessionNumber box="[246,366,1976,2003]" httpUri="https://www.ebi.ac.uk/ena/browser/api/embl/OP549027" pageId="2" pageNumber="191">OP549027</accessionNumber>
(ITS).
</materialsCitation>
<materialsCitation accessionNumber="OP549027, OP549028, OP549025" collectingDate="2019-08-16" collectingDateMax="2021-08-08" collectingDateMin="2019-08-16" country="Pakistan" county="Indus River" elevation="134" latitude="31.988155" location="Muzaffargarh" longLatPrecision="1" longitude="71.99052" municipality="Kot Addu" pageId="2" pageNumber="191" specimenCount="1" stateProvince="Punjab">
Same location,
<date box="[609,793,1976,2002]" pageId="2" pageNumber="191" value="2021-08-03">
<collectingDate box="[609,793,1976,2002]" pageId="2" pageNumber="191" value="2021-08-03">August 03, 2021</collectingDate>
</date>
,
<emphasis box="[801,1060,1976,2002]" italics="true" pageId="2" pageNumber="191">Muhammad Haqnawaz</emphasis>
(LAH37570).GenBank
<accessionNumber box="[1332,1451,1976,2003]" httpUri="https://www.ebi.ac.uk/ena/browser/api/embl/OP549028" pageId="2" pageNumber="191">OP549028</accessionNumber>
(ITS). Khushab, Noorpur Thal, Peelowains (
<geoCoordinate box="[641,816,2012,2039]" degrees="31" direction="north" minutes="59" orientation="latitude" pageId="2" pageNumber="191" precision="1" seconds="17.36" value="31.988155">31°5917.36”N</geoCoordinate>
,
<geoCoordinate box="[829,1001,2012,2039]" degrees="71" direction="east" minutes="59" orientation="longitude" pageId="2" pageNumber="191" precision="1" seconds="25.86" value="71.99052">71°5925.86”E</geoCoordinate>
,
<elevation box="[1014,1147,2012,2039]" metricMagnitude="2" metricUnit="m" metricValue="1.91" pageId="2" pageNumber="191" unit="m" value="191.0">
<quantity box="[1014,1086,2012,2039]" metricMagnitude="2" metricUnit="m" metricValue="1.91" pageId="2" pageNumber="191" unit="m" value="191.0">191 m</quantity>
a.s.l.
</elevation>
),
<date box="[1169,1361,2012,2038]" pageId="2" pageNumber="191" value="2021-08-08">
<collectingDate box="[1169,1361,2012,2038]" pageId="2" pageNumber="191" value="2021-08-08">August 08, 2021</collectingDate>
</date>
,
<emphasis italics="true" pageId="2" pageNumber="191">Zaman Khan</emphasis>
(LAH37571). GenBank
<accessionNumber box="[477,596,2048,2075]" httpUri="https://www.ebi.ac.uk/ena/browser/api/embl/OP549025" pageId="2" pageNumber="191">OP549025</accessionNumber>
(ITS)
</materialsCitation>
.
</paragraph>
</subSubSection>
</treatment>
</document>