219 lines
40 KiB
XML
219 lines
40 KiB
XML
<document id="91B5317B7D40DDCCAA71B7A18FB83129" ID-DOI="10.11646/zootaxa.3887.2.3" ID-GBIF-Dataset="190e4917-516a-4032-809a-42471bf7a52c" ID-ISSN="1175-5326" ID-Zenodo-Dep="229268" ID-ZooBank="B697D6BA-836B-44E2-A8D9-07661554FE59" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.tables_requiresApprovalFor="existingObjects,plazi" IM.taxonomicNames_approvedBy="felipe" checkinTime="1460976521246" checkinUser="plazi" docAuthor="Laamiri, Sayef" docDate="2014" docId="038887C4FFC3FFD3B3BBFF1580F042E5" docLanguage="en" docName="zt03887p190.pdf" docOrigin="Zootaxa 3887 (2)" docStyle="DocumentStyle:647186512141C8FC8976D5BCC54AEB7D.9:Zootaxa.2013-.journal_article" docStyleId="647186512141C8FC8976D5BCC54AEB7D" docStyleName="Zootaxa.2013-.journal_article" docStyleVersion="9" docTitle="Ceratomyxa herouardi Georgevitch 1916" docType="treatment" docVersion="8" lastPageNumber="165" masterDocId="FFB1FFBCFFC4FFDBB32CFF8281304349" masterDocTitle="New observations on Myxozoa of the goldline sea bream Sarpa salpa L. 1758 (Teleostei: Sparidae) from the Mediterranean coast of Tunisia" masterLastPageNumber="190" masterPageNumber="157" pageNumber="164" updateTime="1698559735493" updateUser="plazi">
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<mods:title id="90DFFD768E074D00861008F80F427572">New observations on Myxozoa of the goldline sea bream Sarpa salpa L. 1758 (Teleostei: Sparidae) from the Mediterranean coast of Tunisia</mods:title>
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<mods:namePart id="F55FC8FE0EBF356E64FF89009EDCDBA6">Laamiri, Sayef</mods:namePart>
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<mods:title id="6EA5684A900168085D3769668730B23A">Zootaxa</mods:title>
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<mods:part id="96FDF880106DE8CFE259A37B269EECE5">
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<mods:date id="EED94D4AF1EBBA78F76205BAF44D55E6">2014</mods:date>
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<mods:detail id="5204810388A2FE25C6F9EE410601BE8A" type="volume">
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<mods:number id="9D7CC8DC443A4C4560B60246BDDDEDC9">3887</mods:number>
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<mods:detail id="B438D11B1BD737DA1DD96C32F4DAC050" type="issue">
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<mods:number id="43D54A5EA72AC64A8B024FFBA77FCC99">2</mods:number>
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<mods:identifier id="2F2050C861C8980C5039A4875373155C" type="DOI">10.11646/zootaxa.3887.2.3</mods:identifier>
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<mods:identifier id="10DF9C733486ACDF75B57E85A10071B4" type="GBIF-Dataset">190e4917-516a-4032-809a-42471bf7a52c</mods:identifier>
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<mods:identifier id="1E71191EE25857D865075FC4326CF187" type="ISSN">1175-5326</mods:identifier>
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<treatment id="038887C4FFC3FFD3B3BBFF1580F042E5" ID-DOI="http://doi.org/10.5281/zenodo.5626244" ID-GBIF-Taxon="119594200" ID-Zenodo-Dep="5626244" LSID="urn:lsid:plazi:treatment:038887C4FFC3FFD3B3BBFF1580F042E5" httpUri="http://treatment.plazi.org/id/038887C4FFC3FFD3B3BBFF1580F042E5" lastPageId="8" lastPageNumber="165" pageId="7" pageNumber="164">
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<subSubSection id="C33B6559FFC3FFDCB3BBFF1583A343FB" box="[151,659,151,178]" pageId="7" pageNumber="164" type="nomenclature">
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<paragraph id="8B9E36D2FFC3FFDCB3BBFF1583A343FB" blockId="7.[151,659,151,178]" box="[151,659,151,178]" pageId="7" pageNumber="164">
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<heading id="D0D681BEFFC3FFDCB3BBFF1583A343FB" bold="true" box="[151,659,151,178]" fontSize="11" level="1" pageId="7" pageNumber="164" reason="1">
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<taxonomicName id="4C214D51FFC3FFDCB3BBFF1583A343FB" authority="Georgevitch, 1916" authorityName="Georgevitch" authorityYear="1916" box="[151,659,151,178]" class="Myxosporea" family="Ceratomyxidae" genus="Ceratomyxa" kingdom="Animalia" order="Bivalvulida" pageId="7" pageNumber="164" phylum="Myxozoa" rank="species" species="herouardi">
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<emphasis id="B955EAC0FFC3FFDCB3BBFF1583A343FB" bold="true" box="[151,659,151,178]" pageId="7" pageNumber="164">
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<emphasis id="B955EAC0FFC3FFDCB3BBFF15809943F8" bold="true" box="[151,425,151,177]" italics="true" pageId="7" pageNumber="164">Ceratomyxa herouardi</emphasis>
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Georgévitch, 1916
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</emphasis>
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</taxonomicName>
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</heading>
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</paragraph>
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</subSubSection>
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<subSubSection id="C33B6559FFC3FFD3B3BBFF5C80F042E5" lastPageId="8" lastPageNumber="165" pageId="7" pageNumber="164" type="materials_examined">
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<paragraph id="8B9E36D2FFC3FFDCB3BBFF5C857043BE" blockId="7.[151,1437,222,643]" box="[151,1088,222,247]" pageId="7" pageNumber="164">
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<emphasis id="B955EAC0FFC3FFDCB3BBFF5C802343BE" bold="true" box="[151,275,222,247]" pageId="7" pageNumber="164">
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||
<typeStatus id="549A8870FFC3FFDCB3BBFF5C81E243BE" box="[151,210,222,247]" pageId="7" pageNumber="164">Type</typeStatus>
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host:
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</emphasis>
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<taxonomicName id="4C214D51FFC3FFDCB237FF5D836543BE" authority="Linnaeus, 1758" authorityName="Linnaeus" authorityYear="1758" box="[283,597,222,247]" class="Actinopterygii" family="Sparidae" genus="Sarpa" kingdom="Animalia" order="Perciformes" pageId="7" pageNumber="164" phylum="Chordata" rank="species" species="salpa">
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<emphasis id="B955EAC0FFC3FFDCB237FF5D809043BE" box="[283,416,222,247]" italics="true" pageId="7" pageNumber="164">Sarpa salpa</emphasis>
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Linnaeus, 1758
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</taxonomicName>
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goldline sea bream (
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<taxonomicName id="4C214D51FFC3FFDCB012FF5C82FC43BE" box="[830,972,222,247]" class="Actinopterygii" kingdom="Animalia" order="Perciformes" pageId="7" pageNumber="164" phylum="Chordata" rank="order">Perciformes</taxonomicName>
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:
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<taxonomicName id="4C214D51FFC3FFDCB0FAFF5C850A43BE" box="[982,1082,222,247]" class="Actinopterygii" family="Sparidae" kingdom="Animalia" order="Perciformes" pageId="7" pageNumber="164" phylum="Chordata" rank="family">Sparidae</taxonomicName>
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)
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</paragraph>
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<paragraph id="8B9E36D2FFC3FFDCB3EBFE8080E54276" blockId="7.[151,1437,222,643]" pageId="7" pageNumber="164">
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<emphasis id="B955EAC0FFC3FFDCB3EBFE80804A4252" bold="true" box="[199,378,258,283]" pageId="7" pageNumber="164">
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<typeStatus id="549A8870FFC3FFDCB3EBFE8080324252" box="[199,258,258,283]" pageId="7" pageNumber="164">Type</typeStatus>
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localities:
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</emphasis>
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Mediterranean off
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<collectingCountry id="F3367642FFC3FFDCB17FFE80839B4252" box="[595,683,258,283]" name="Tunisia" pageId="7" pageNumber="164">Tunisia</collectingCountry>
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: Location 1: Gulf of
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<collectingCountry id="F3367642FFC3FFDCB0B9FE8082E44252" box="[917,980,258,283]" name="Tunisia" pageId="7" pageNumber="164">Tunis</collectingCountry>
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(
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<geoCoordinate id="EE155015FFC3FFDCB0C8FE8085774252" box="[996,1095,258,283]" direction="north" orientation="latitude" pageId="7" pageNumber="164" precision="925" value="36.75">36°45’N</geoCoordinate>
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,
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<geoCoordinate id="EE155015FFC3FFDCB77FFE8085844252" box="[1107,1204,258,283]" direction="east" orientation="longitude" pageId="7" pageNumber="164" precision="925" value="10.25">10°15’E</geoCoordinate>
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); Location 2: Bay of Bizerte (
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<geoCoordinate id="EE155015FFC3FFDCB3DBFEA580534277" box="[247,355,295,319]" direction="north" orientation="latitude" pageId="7" pageNumber="164" precision="925" value="37.333332">37°20’ N</geoCoordinate>
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,
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<geoCoordinate id="EE155015FFC3FFDCB242FEA480F84276" box="[366,456,294,319]" direction="east" orientation="longitude" pageId="7" pageNumber="164" precision="925" value="9.883333">9°53’ E</geoCoordinate>
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).
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</paragraph>
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<paragraph id="8B9E36D2FFC3FFDCB3EBFEC8835A422A" blockId="7.[151,1437,222,643]" box="[199,618,330,355]" pageId="7" pageNumber="164">
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<emphasis id="B955EAC0FFC3FFDCB3EBFEC880BB422A" bold="true" box="[199,395,330,355]" pageId="7" pageNumber="164">Site of infection:</emphasis>
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Within gall bladder
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</paragraph>
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<paragraph id="8B9E36D2FFC3FFDCB3EBFEEC82D3415E" blockId="7.[151,1437,222,643]" pageId="7" pageNumber="164">
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<emphasis id="B955EAC0FFC3FFDCB3EBFEEC806242CE" bold="true" box="[199,338,366,391]" pageId="7" pageNumber="164">Prevalence:</emphasis>
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The overall prevalence is 28.2% (93/330) (
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<figureCitation id="131A2A57FFC3FFDCB012FEEC82B342CE" box="[830,899,366,391]" captionStart="FIGURE 9" captionStartId="25.[151,250,783,805]" captionTargetBox="[325,1259,201,752]" captionTargetId="figure@25.[319,1267,195,759]" captionTargetPageId="25" captionText="FIGURE 9. Prevalence of species of myxozoans from 2 localities, Gulf of Tunis and Bay of Bizerte. The total prevalence determined within the pooled sample of 330 goldline sea bream (Sarpa salpa)." httpUri="https://zenodo.org/record/229279/files/figure.png" pageId="7" pageNumber="164">Fig. 9</figureCitation>
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). At location 1, the prevalence of infection was 33.3% (70/210) distributed as following, 03/2012: 36.7% (11/30); 04/2012: 30% (9/30); 05/2012: 40% (12/30); 06/ 2012: 46.7% (14/30); 07/2012: 23.3% (7/30); 08/2012: 30% (9/30); 05/2013: 25% (5/20); 06/2013: 30% (3/10). At location 2, the prevalence of infection is 19.2% (23/120) distributed as following, 03/2013: 13.3% (4/30); 04/2013: 13.3% (4/30); 05/2013: 26.7% (8/30); 06/2013: 23.3% (7/30) (see Table 4).
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</paragraph>
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<paragraph id="8B9E36D2FFC3FFDCB3EBFDA085584172" blockId="7.[151,1437,222,643]" box="[199,1128,546,571]" pageId="7" pageNumber="164">
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<emphasis id="B955EAC0FFC3FFDCB3EBFDA080B24172" bold="true" box="[199,386,546,571]" pageId="7" pageNumber="164">Mean intensity:</emphasis>
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211.2 ± 50.6 spores/infected fish (++++++) (
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<figureCitation id="131A2A57FFC3FFDCB053FDA082F24172" box="[895,962,546,571]" captionStart="FIGURE 9" captionStartId="25.[151,250,783,805]" captionTargetBox="[325,1259,201,752]" captionTargetId="figure@25.[319,1267,195,759]" captionTargetPageId="25" captionText="FIGURE 9. Prevalence of species of myxozoans from 2 localities, Gulf of Tunis and Bay of Bizerte. The total prevalence determined within the pooled sample of 330 goldline sea bream (Sarpa salpa)." httpUri="https://zenodo.org/record/229279/files/figure.png" pageId="7" pageNumber="164">Fig. 9</figureCitation>
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) (see Table 4).
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</paragraph>
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<paragraph id="8B9E36D2FFC3FFDCB3EBFDC482E241CA" blockId="7.[151,1437,222,643]" pageId="7" pageNumber="164">
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<emphasis id="B955EAC0FFC3FFDCB3EBFDC480494116" bold="true" box="[199,377,582,607]" pageId="7" pageNumber="164">Type-material:</emphasis>
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Digitized photos of
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<typeStatus id="549A8870FFC3FFDCB15DFDC583F84116" box="[625,712,583,607]" pageId="7" pageNumber="164" type="syntype">syntype</typeStatus>
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spores were deposited in the parasitological collection of the Museum National d’Histoire Naturelle (
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<collectionCode id="ED30AE17FFC3FFDCB17BFDE9838241CA" LSID="urn:lsid:biocol.org:col:34988" box="[599,690,619,643]" httpUri="http://biocol.org/urn:lsid:biocol.org:col:34988" name="Museum National d'Histoire Naturelle" pageId="7" pageNumber="164">MNHN</collectionCode>
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), Paris, Coll. No. ZS 117.
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</paragraph>
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<paragraph id="8B9E36D2FFC3FFDCB3BBFD30802A4182" blockId="7.[151,1437,690,1327]" box="[151,282,690,715]" pageId="7" pageNumber="164">Description</paragraph>
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<paragraph id="8B9E36D2FFC3FFDCB3EBFD5485D14746" blockId="7.[151,1437,690,1327]" pageId="7" pageNumber="164">
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<emphasis id="B955EAC0FFC3FFDCB3EBFD5480A941A6" bold="true" box="[199,409,726,751]" pageId="7" pageNumber="164">Vegetative stages.</emphasis>
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Trophozoites (n = 100) were freely floating in bile of the gallbladder in different stages of maturation and some seen attached to gall bladder epithelium. Polymorphous with great variety of shape and size, elongated with same breadth or tapering to one end or club-shaped with roundish enlargements (
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<figureCitation id="131A2A57FFC3FFDCB7CFFC9C840C407E" box="[1251,1340,798,823]" captionStart="FIGURE 3 A – F" captionStartId="8.[151,250,1689,1711]" captionTargetBox="[351,1239,484,1665]" captionTargetId="figure@8.[151,1436,466,1683]" captionTargetPageId="8" captionText="FIGURE 3 A – F. Photomicrographs of Ceratomyxa herouardi from the gall bladder of Sarpa salpa. (A) Round trophozoite (RT) attached to other polymorphous, notice the presence of inner generative cells and long filopodia (F). (B) Fresh smear of infested bile with the presence of elongated trophozoites (ET) contained pseudoplasmodi (pp), disporic plasmodia (P) and mature spores (ms). (C) Big rounded polysporic trophozoïtes (RT) contained several pseudoplasmodia (pp). (D) Part of trophozoite showing in Fig. C, presented the pseudoplasmodia (pp) and disporic plasmodia (P) containing immature spores (is). (E – F) Subspherical and pyriform trophozoites (PT) unequal in size attached to each other with formation of roundish disporic plasmodia (P) according to mechanism of endogenous or exogenous budding, notice of a disporic plasmodium of atypical spores (P *) in F. Scale bar = 50 µm in A – C; 20 µm in D – F;" httpUri="https://zenodo.org/record/229271/files/figure.png" pageId="7" pageNumber="164">Fig. 3B</figureCitation>
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). Young trophozoïtes were spherical or pyriform (
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<figureCitation id="131A2A57FFC3FFDCB1A3FCC082284012" box="[655,792,834,859]" captionStart="FIGURE 3 A – F" captionStartId="8.[151,250,1689,1711]" captionTargetBox="[351,1239,484,1665]" captionTargetId="figure@8.[151,1436,466,1683]" captionTargetPageId="8" captionText="FIGURE 3 A – F. Photomicrographs of Ceratomyxa herouardi from the gall bladder of Sarpa salpa. (A) Round trophozoite (RT) attached to other polymorphous, notice the presence of inner generative cells and long filopodia (F). (B) Fresh smear of infested bile with the presence of elongated trophozoites (ET) contained pseudoplasmodi (pp), disporic plasmodia (P) and mature spores (ms). (C) Big rounded polysporic trophozoïtes (RT) contained several pseudoplasmodia (pp). (D) Part of trophozoite showing in Fig. C, presented the pseudoplasmodia (pp) and disporic plasmodia (P) containing immature spores (is). (E – F) Subspherical and pyriform trophozoites (PT) unequal in size attached to each other with formation of roundish disporic plasmodia (P) according to mechanism of endogenous or exogenous budding, notice of a disporic plasmodium of atypical spores (P *) in F. Scale bar = 50 µm in A – C; 20 µm in D – F;" httpUri="https://zenodo.org/record/229271/files/figure.png" pageId="7" pageNumber="164">Figs. 3E–F</figureCitation>
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). Trophozoïtes were attached to each other by their pseudopodia while other possessed a long pseudopodia-like filopodia probably used for their motility (
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<figureCitation id="131A2A57FFC3FFDCB61FFCE484BF4036" box="[1331,1423,870,895]" captionStart="FIGURE 3 A – F" captionStartId="8.[151,250,1689,1711]" captionTargetBox="[351,1239,484,1665]" captionTargetId="figure@8.[151,1436,466,1683]" captionTargetPageId="8" captionText="FIGURE 3 A – F. Photomicrographs of Ceratomyxa herouardi from the gall bladder of Sarpa salpa. (A) Round trophozoite (RT) attached to other polymorphous, notice the presence of inner generative cells and long filopodia (F). (B) Fresh smear of infested bile with the presence of elongated trophozoites (ET) contained pseudoplasmodi (pp), disporic plasmodia (P) and mature spores (ms). (C) Big rounded polysporic trophozoïtes (RT) contained several pseudoplasmodia (pp). (D) Part of trophozoite showing in Fig. C, presented the pseudoplasmodia (pp) and disporic plasmodia (P) containing immature spores (is). (E – F) Subspherical and pyriform trophozoites (PT) unequal in size attached to each other with formation of roundish disporic plasmodia (P) according to mechanism of endogenous or exogenous budding, notice of a disporic plasmodium of atypical spores (P *) in F. Scale bar = 50 µm in A – C; 20 µm in D – F;" httpUri="https://zenodo.org/record/229271/files/figure.png" pageId="7" pageNumber="164">Fig. 3A</figureCitation>
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). Protoplasm homogeneous with fine refractile granules and inner generative cells. Subspherical to spherical plasmodia measuring 30.23 ± 5.5 (22–40) µm in length and 31.8 ± 5.1 (27–44.5) µm in width (n = 50). Disporous, each plasmodium contained ordinarily two identical spores (
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<figureCitation id="131A2A57FFC3FFDCB042FC5082DA40A2" box="[878,1002,978,1003]" captionStart="FIGURE 3 A – F" captionStartId="8.[151,250,1689,1711]" captionTargetBox="[351,1239,484,1665]" captionTargetId="figure@8.[151,1436,466,1683]" captionTargetPageId="8" captionText="FIGURE 3 A – F. Photomicrographs of Ceratomyxa herouardi from the gall bladder of Sarpa salpa. (A) Round trophozoite (RT) attached to other polymorphous, notice the presence of inner generative cells and long filopodia (F). (B) Fresh smear of infested bile with the presence of elongated trophozoites (ET) contained pseudoplasmodi (pp), disporic plasmodia (P) and mature spores (ms). (C) Big rounded polysporic trophozoïtes (RT) contained several pseudoplasmodia (pp). (D) Part of trophozoite showing in Fig. C, presented the pseudoplasmodia (pp) and disporic plasmodia (P) containing immature spores (is). (E – F) Subspherical and pyriform trophozoites (PT) unequal in size attached to each other with formation of roundish disporic plasmodia (P) according to mechanism of endogenous or exogenous budding, notice of a disporic plasmodium of atypical spores (P *) in F. Scale bar = 50 µm in A – C; 20 µm in D – F;" httpUri="https://zenodo.org/record/229271/files/figure.png" pageId="7" pageNumber="164">Figs.3C–E</figureCitation>
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), or polysporous, with formation of numerous disporic plasmodia attached to each other within the trophozoïte mother (
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<figureCitation id="131A2A57FFC3FFDCB762FC7485E54746" box="[1102,1237,1014,1039]" captionStart="FIGURE 3 A – F" captionStartId="8.[151,250,1689,1711]" captionTargetBox="[351,1239,484,1665]" captionTargetId="figure@8.[151,1436,466,1683]" captionTargetPageId="8" captionText="FIGURE 3 A – F. Photomicrographs of Ceratomyxa herouardi from the gall bladder of Sarpa salpa. (A) Round trophozoite (RT) attached to other polymorphous, notice the presence of inner generative cells and long filopodia (F). (B) Fresh smear of infested bile with the presence of elongated trophozoites (ET) contained pseudoplasmodi (pp), disporic plasmodia (P) and mature spores (ms). (C) Big rounded polysporic trophozoïtes (RT) contained several pseudoplasmodia (pp). (D) Part of trophozoite showing in Fig. C, presented the pseudoplasmodia (pp) and disporic plasmodia (P) containing immature spores (is). (E – F) Subspherical and pyriform trophozoites (PT) unequal in size attached to each other with formation of roundish disporic plasmodia (P) according to mechanism of endogenous or exogenous budding, notice of a disporic plasmodium of atypical spores (P *) in F. Scale bar = 50 µm in A – C; 20 µm in D – F;" httpUri="https://zenodo.org/record/229271/files/figure.png" pageId="7" pageNumber="164">Figs. 3C–D</figureCitation>
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).
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</paragraph>
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<paragraph id="8B9E36D2FFC3FFDCB3EBFB9880BE4666" blockId="7.[151,1437,690,1327]" pageId="7" pageNumber="164">
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<emphasis id="B955EAC0FFC3FFDCB3EBFB988027477A" bold="true" box="[199,279,1050,1075]" pageId="7" pageNumber="164">Spores</emphasis>
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(n = 100 fresh spores). Mature spores were elliptic to hemispherical broadly with round ends in sutural view (
|
||
<figureCitation id="131A2A57FFC3FFDCB3CDFBBC80B3471E" box="[225,387,1086,1111]" captionStart-0="FIGURE 3 A – F" captionStart-1="FIGURE 3 G – N" captionStartId-0="8.[151,250,1689,1711]" captionStartId-1="9.[151,250,1415,1437]" captionTargetBox-0="[351,1239,484,1665]" captionTargetBox-1="[221,1365,212,1414]" captionTargetId-0="figure@8.[151,1436,466,1683]" captionTargetId-1="figure@9.[221,1365,193,1422]" captionTargetPageId-0="8" captionTargetPageId-1="9" captionText-0="FIGURE 3 A – F. Photomicrographs of Ceratomyxa herouardi from the gall bladder of Sarpa salpa. (A) Round trophozoite (RT) attached to other polymorphous, notice the presence of inner generative cells and long filopodia (F). (B) Fresh smear of infested bile with the presence of elongated trophozoites (ET) contained pseudoplasmodi (pp), disporic plasmodia (P) and mature spores (ms). (C) Big rounded polysporic trophozoïtes (RT) contained several pseudoplasmodia (pp). (D) Part of trophozoite showing in Fig. C, presented the pseudoplasmodia (pp) and disporic plasmodia (P) containing immature spores (is). (E – F) Subspherical and pyriform trophozoites (PT) unequal in size attached to each other with formation of roundish disporic plasmodia (P) according to mechanism of endogenous or exogenous budding, notice of a disporic plasmodium of atypical spores (P *) in F. Scale bar = 50 µm in A – C; 20 µm in D – F;" captionText-1="FIGURE 3 G – N. Photomicrographs of Ceratomyxa herouardi from the gall bladder of Sarpa salpa. (G) Fresh smear of heavy infested bile with sporogonic stages and mature spores. (H) Plasmodium (P) of premature spores showing the sporoplasm nuclei (sn). (I) Mature spore in sutural view showing the sub-spherical polar capsules (pc). (J) Mature spore in lateral view. (K) Mature spore in apical view. (L) Atypical spore with three polar capsules and three shell valves. (M – N) Different view of mature spores stained with Giemsa showing the distinct polar capsule (pc) and the suture line (sl). M, sutural view; N, apical view. Scale bar = 40 µm in G; 10 µm in H-N." httpUri-0="https://zenodo.org/record/229271/files/figure.png" httpUri-1="https://zenodo.org/record/229272/files/figure.png" pageId="7" pageNumber="164">
|
||
Figs.
|
||
<date id="FF9F1012FFC3FFDCB209FBBD800B471E" box="[293,315,1087,1111]" pageId="7" pageNumber="164">3I</date>
|
||
–K,M
|
||
</figureCitation>
|
||
,
|
||
<figureCitation id="131A2A57FFC3FFDCB2A4FBBD80A5471E" box="[392,405,1087,1111]" captionStart="FIGURE 8" captionStartId="24.[151,250,1200,1222]" captionTargetBox="[277,1304,161,1171]" captionTargetId="figure@24.[266,1321,155,1184]" captionTargetPageId="24" captionText="FIGURE 8. Line drawing of Myxozoan species infecting the goldline sea bream Sarpa salpa from the North coast of Tunisia. (A) Ceratomyxa arcuata Thélohan, 1892; (B) Ceratomyxa pallida Thélohan, 1895; (C) Ceratomyxa herouardi Georgévitch, 1916; (D) Ceratomyxa sp. 1; (E) Ceratomyxa sp. 2; (F) Ceratomyxa sp. 3; (G) Henneguya sp. Scale bar = 10 µm." httpUri="https://zenodo.org/record/229278/files/figure.png" pageId="7" pageNumber="164">8</figureCitation>
|
||
C), measuring 10.5 ± 1.2 (8–12) Μm in length and 21.6 ± 1.6 (20–24) Μm in thickness. Posterior angle was flattened to straight 172.5 ± 6.8 (165–180°). The suture line divided the spore in two equal valves smoothly ovoid in lateral view (
|
||
<figureCitation id="131A2A57FFC3FFDCB17FFB04839347D6" box="[595,675,1158,1183]" captionStart-0="FIGURE 3 A – F" captionStart-1="FIGURE 3 G – N" captionStartId-0="8.[151,250,1689,1711]" captionStartId-1="9.[151,250,1415,1437]" captionTargetBox-0="[351,1239,484,1665]" captionTargetBox-1="[221,1365,212,1414]" captionTargetId-0="figure@8.[151,1436,466,1683]" captionTargetId-1="figure@9.[221,1365,193,1422]" captionTargetPageId-0="8" captionTargetPageId-1="9" captionText-0="FIGURE 3 A – F. Photomicrographs of Ceratomyxa herouardi from the gall bladder of Sarpa salpa. (A) Round trophozoite (RT) attached to other polymorphous, notice the presence of inner generative cells and long filopodia (F). (B) Fresh smear of infested bile with the presence of elongated trophozoites (ET) contained pseudoplasmodi (pp), disporic plasmodia (P) and mature spores (ms). (C) Big rounded polysporic trophozoïtes (RT) contained several pseudoplasmodia (pp). (D) Part of trophozoite showing in Fig. C, presented the pseudoplasmodia (pp) and disporic plasmodia (P) containing immature spores (is). (E – F) Subspherical and pyriform trophozoites (PT) unequal in size attached to each other with formation of roundish disporic plasmodia (P) according to mechanism of endogenous or exogenous budding, notice of a disporic plasmodium of atypical spores (P *) in F. Scale bar = 50 µm in A – C; 20 µm in D – F;" captionText-1="FIGURE 3 G – N. Photomicrographs of Ceratomyxa herouardi from the gall bladder of Sarpa salpa. (G) Fresh smear of heavy infested bile with sporogonic stages and mature spores. (H) Plasmodium (P) of premature spores showing the sporoplasm nuclei (sn). (I) Mature spore in sutural view showing the sub-spherical polar capsules (pc). (J) Mature spore in lateral view. (K) Mature spore in apical view. (L) Atypical spore with three polar capsules and three shell valves. (M – N) Different view of mature spores stained with Giemsa showing the distinct polar capsule (pc) and the suture line (sl). M, sutural view; N, apical view. Scale bar = 40 µm in G; 10 µm in H-N." httpUri-0="https://zenodo.org/record/229271/files/figure.png" httpUri-1="https://zenodo.org/record/229272/files/figure.png" pageId="7" pageNumber="164">Fig. 3J</figureCitation>
|
||
). Polar capsules were nearly spherical to sub-spherical 3.91 ± 0.25 (3.5–4.5) Μm in length and 3.89 ± 0.27 (3.5–4.5) Μm in width (n = 100). The polar filament formed five to six turns arranged along the longitudinal axis of the capsule. A binucleate sporoplasm almost filled the spore cavity and was generally distributed symmetrically. Occasionally, aberrant spores with 3 polar capsules and 3 valves were observed (
|
||
<figureCitation id="131A2A57FFC3FFDCB227FA94805A4666" box="[267,362,1302,1327]" captionStart="FIGURE 3 A – F" captionStartId="8.[151,250,1689,1711]" captionTargetBox="[351,1239,484,1665]" captionTargetId="figure@8.[151,1436,466,1683]" captionTargetPageId="8" captionText="FIGURE 3 A – F. Photomicrographs of Ceratomyxa herouardi from the gall bladder of Sarpa salpa. (A) Round trophozoite (RT) attached to other polymorphous, notice the presence of inner generative cells and long filopodia (F). (B) Fresh smear of infested bile with the presence of elongated trophozoites (ET) contained pseudoplasmodi (pp), disporic plasmodia (P) and mature spores (ms). (C) Big rounded polysporic trophozoïtes (RT) contained several pseudoplasmodia (pp). (D) Part of trophozoite showing in Fig. C, presented the pseudoplasmodia (pp) and disporic plasmodia (P) containing immature spores (is). (E – F) Subspherical and pyriform trophozoites (PT) unequal in size attached to each other with formation of roundish disporic plasmodia (P) according to mechanism of endogenous or exogenous budding, notice of a disporic plasmodium of atypical spores (P *) in F. Scale bar = 50 µm in A – C; 20 µm in D – F;" httpUri="https://zenodo.org/record/229271/files/figure.png" pageId="7" pageNumber="164">Figs. 3F</figureCitation>
|
||
,
|
||
<figureCitation id="131A2A57FFC3FFDCB242FA9580B24666" box="[366,386,1303,1327]" captionStart="FIGURE 3 G – N" captionStartId="9.[151,250,1415,1437]" captionTargetBox="[221,1365,212,1414]" captionTargetId="figure@9.[221,1365,193,1422]" captionTargetPageId="9" captionText="FIGURE 3 G – N. Photomicrographs of Ceratomyxa herouardi from the gall bladder of Sarpa salpa. (G) Fresh smear of heavy infested bile with sporogonic stages and mature spores. (H) Plasmodium (P) of premature spores showing the sporoplasm nuclei (sn). (I) Mature spore in sutural view showing the sub-spherical polar capsules (pc). (J) Mature spore in lateral view. (K) Mature spore in apical view. (L) Atypical spore with three polar capsules and three shell valves. (M – N) Different view of mature spores stained with Giemsa showing the distinct polar capsule (pc) and the suture line (sl). M, sutural view; N, apical view. Scale bar = 40 µm in G; 10 µm in H-N." httpUri="https://zenodo.org/record/229272/files/figure.png" pageId="7" pageNumber="164">L</figureCitation>
|
||
).
|
||
</paragraph>
|
||
<paragraph id="8B9E36D2FFC3FFDCB3BBFADC81CB463E" blockId="7.[151,1436,1374,1939]" box="[151,251,1374,1399]" pageId="7" pageNumber="164">Remarks</paragraph>
|
||
<paragraph id="8B9E36D2FFC3FFDCB3EBFA00830444DA" blockId="7.[151,1436,1374,1939]" pageId="7" pageNumber="164">
|
||
The first description of
|
||
<taxonomicName id="4C214D51FFC3FFDCB2E1FA01836C46D2" box="[461,604,1410,1435]" class="Myxosporea" family="Ceratomyxidae" genus="Ceratomyxa" kingdom="Animalia" order="Bivalvulida" pageId="7" pageNumber="164" phylum="Myxozoa" rank="species" species="herouardi">
|
||
<emphasis id="B955EAC0FFC3FFDCB2E1FA01836C46D2" box="[461,604,1410,1435]" italics="true" pageId="7" pageNumber="164">C. herouardi</emphasis>
|
||
</taxonomicName>
|
||
was illustrated by
|
||
<bibRefCitation id="EFB04B23FFC3FFDCB001FA00853E46D2" author="Georgevitch" box="[813,1038,1410,1435]" pageId="7" pageNumber="164" refString="Georgevitch, J. (1916 a) Note sur les myxosporidies des poissons de la baie de Ville franche et de Monaco. Bulletin de l'Institut Oceanographique de Monaco, 322, 1 - 7." type="journal article" year="1916" yearSuffix="a">Georgévitch (1916a</bibRefCitation>
|
||
,
|
||
<bibRefCitation id="EFB04B23FFC3FFDCB735FA00855246D2" author="Georgevitch" box="[1049,1122,1410,1435]" pageId="7" pageNumber="164" refString="Georgevitch, J. (1916 b) Sur les diverses formes de Ceratomyxa herouardi Georgevitch.. Comptes rendus hebdomadaires des seances de l'Academie des sciences, 163, 717 - 719." type="journal article" year="1916" yearSuffix="b">1916b</bibRefCitation>
|
||
,
|
||
<bibRefCitation id="EFB04B23FFC3FFDCB741FA00858446D2" author="Georgevitch" box="[1133,1204,1410,1435]" pageId="7" pageNumber="164" refString="Georgevitch, J. (1916 c) Sur le cycle evolutif de Ceratomyxa herouardi Georgevitch. Comptes rendus hebdomadaires des seances de l'Academie des sciences, 163, 983 - 985." type="journal article" year="1916" yearSuffix="c">1916c</bibRefCitation>
|
||
,
|
||
<bibRefCitation id="EFB04B23FFC3FFDCB793FA0185C846D2" author="Georgevitch" box="[1215,1272,1411,1435]" pageId="7" pageNumber="164" refString="Georgevitch, J. (1917) Recherches sur le developpement de Ceratomyxa herouardi Georgevitch. Archives de zoologie experimentale et generale, 56, 375 - 399." type="journal article" year="1917">1917</bibRefCitation>
|
||
) from
|
||
<taxonomicName id="4C214D51FFC3FFDCB668FA0184AB46D2" box="[1348,1435,1410,1435]" class="Actinopterygii" family="Sparidae" genus="Sarpa" kingdom="Animalia" order="Perciformes" pageId="7" pageNumber="164" phylum="Chordata" rank="species" species="salpa">
|
||
<emphasis id="B955EAC0FFC3FFDCB668FA0184AB46D2" box="[1348,1435,1410,1435]" italics="true" pageId="7" pageNumber="164">S. salpa</emphasis>
|
||
</taxonomicName>
|
||
captured in Mediterranean coast of
|
||
<collectingCountry id="F3367642FFC3FFDCB111FA2583AC46F7" box="[573,668,1447,1470]" name="Monaco" pageId="7" pageNumber="164">Monaco</collectingCountry>
|
||
,
|
||
<collectingCountry id="F3367642FFC3FFDCB187FA2583CB46F7" box="[683,763,1447,1470]" name="France" pageId="7" pageNumber="164">France</collectingCountry>
|
||
. The description was focused on the huge variety of the vegetative forms. Georgévitch (1916) declared that
|
||
<taxonomicName id="4C214D51FFC3FFDCB1C9FA49824546AA" box="[741,885,1482,1507]" class="Myxosporea" family="Ceratomyxidae" genus="Ceratomyxa" kingdom="Animalia" order="Bivalvulida" pageId="7" pageNumber="164" phylum="Myxozoa" rank="species" species="herouardi">
|
||
<emphasis id="B955EAC0FFC3FFDCB1C9FA49824546AA" box="[741,885,1482,1507]" italics="true" pageId="7" pageNumber="164">C. herouardi</emphasis>
|
||
</taxonomicName>
|
||
has been especially deserved the most attention from all the
|
||
<taxonomicName id="4C214D51FFC3FFDCB20AFA6D809F454F" box="[294,431,1519,1542]" class="Myxosporea" family="Ceratomyxidae" genus="Ceratomyxa" kingdom="Animalia" order="Bivalvulida" pageId="7" pageNumber="164" phylum="Myxozoa" rank="genus">
|
||
<emphasis id="B955EAC0FFC3FFDCB20AFA6D809F454F" box="[294,431,1519,1542]" italics="true" pageId="7" pageNumber="164">Ceratomyxa</emphasis>
|
||
</taxonomicName>
|
||
spp. identified in that time by its extraordinary vegetative forms that were very diverse (
|
||
<figureCitation id="131A2A57FFC3FFDCB3B3F99080154562" box="[159,293,1554,1579]" captionStart="FIGURE 3 A – F" captionStartId="8.[151,250,1689,1711]" captionTargetBox="[351,1239,484,1665]" captionTargetId="figure@8.[151,1436,466,1683]" captionTargetPageId="8" captionText="FIGURE 3 A – F. Photomicrographs of Ceratomyxa herouardi from the gall bladder of Sarpa salpa. (A) Round trophozoite (RT) attached to other polymorphous, notice the presence of inner generative cells and long filopodia (F). (B) Fresh smear of infested bile with the presence of elongated trophozoites (ET) contained pseudoplasmodi (pp), disporic plasmodia (P) and mature spores (ms). (C) Big rounded polysporic trophozoïtes (RT) contained several pseudoplasmodia (pp). (D) Part of trophozoite showing in Fig. C, presented the pseudoplasmodia (pp) and disporic plasmodia (P) containing immature spores (is). (E – F) Subspherical and pyriform trophozoites (PT) unequal in size attached to each other with formation of roundish disporic plasmodia (P) according to mechanism of endogenous or exogenous budding, notice of a disporic plasmodium of atypical spores (P *) in F. Scale bar = 50 µm in A – C; 20 µm in D – F;" httpUri="https://zenodo.org/record/229271/files/figure.png" pageId="7" pageNumber="164">Figs. 3A–F</figureCitation>
|
||
): round, elliptical, pyriform, elongated and very unequal in size and by its mature spores that could show dissimilar shape in different views. In result of that,
|
||
<bibRefCitation id="EFB04B23FFC3FFDCB031F9B582FE4506" author="Jameson" box="[797,974,1591,1615]" pageId="7" pageNumber="164" refString="Jameson, A. P. (1913) A note on some Myxosporidia collected at Monaco. Bulletin de l'Institut Oceanographique de Monaco, 273, 1 - 4." type="journal article" year="1913">Jameson (1913)</bibRefCitation>
|
||
mentioned the same forms and variety of trophozoïtes in the same host, organ and locality (
|
||
<collectingCountry id="F3367642FFC3FFDCB1EAF9D98215453A" box="[710,805,1627,1651]" name="Monaco" pageId="7" pageNumber="164">Monaco</collectingCountry>
|
||
) without recognized the spores. He couldn’t determined the species and he declared in his study that the form of parasite found in the gall bladder of
|
||
<taxonomicName id="4C214D51FFC3FFDCB7B0F9FD85C445DE" box="[1180,1268,1662,1687]" class="Actinopterygii" family="Sparidae" genus="Sarpa" kingdom="Animalia" order="Perciformes" pageId="7" pageNumber="164" phylum="Chordata" rank="species" species="salpa">
|
||
<emphasis id="B955EAC0FFC3FFDCB7B0F9FD85C445DE" box="[1180,1268,1662,1687]" italics="true" pageId="7" pageNumber="164">S. salpa</emphasis>
|
||
</taxonomicName>
|
||
has something of the appearance of a
|
||
<emphasis id="B955EAC0FFC3FFDCB2B2F920832B45F2" box="[414,539,1698,1723]" italics="true" pageId="7" pageNumber="164">Lepthoteca</emphasis>
|
||
(
|
||
<bibRefCitation id="EFB04B23FFC3FFDCB107F920839F45F2" author="Kudo" box="[555,687,1698,1723]" pageId="7" pageNumber="164" refString="Kudo, R. (1920) Studies on Myxosporidia. A synopsis of genera and species of myxosporidia. Illinois Biological Mongraphs, 5, 1 - 265." type="journal article" year="1920">Kudo 1920</bibRefCitation>
|
||
).
|
||
<bibRefCitation id="EFB04B23FFC3FFDCB1E8F920829245F2" author="Georgevitch" box="[708,930,1698,1723]" pageId="7" pageNumber="164" refString="Georgevitch, J. (1917) Recherches sur le developpement de Ceratomyxa herouardi Georgevitch. Archives de zoologie experimentale et generale, 56, 375 - 399." type="journal article" year="1917">Georgévitch (1917)</bibRefCitation>
|
||
believed that the variety of the trophozoites and the complexity of it developmental cycle according to the process of endogenous or exogenous budding, were an important characteristics to identify
|
||
<taxonomicName id="4C214D51FFC3FFDCB17DF96983D0444A" box="[593,736,1770,1795]" class="Myxosporea" family="Ceratomyxidae" genus="Ceratomyxa" kingdom="Animalia" order="Bivalvulida" pageId="7" pageNumber="164" phylum="Myxozoa" rank="species" species="herouardi">
|
||
<emphasis id="B955EAC0FFC3FFDCB17DF96983D0444A" box="[593,736,1770,1795]" italics="true" pageId="7" pageNumber="164">C. herouardi</emphasis>
|
||
</taxonomicName>
|
||
from the other species of the same genus. This author made a traditional demonstration about the life cycle of
|
||
<taxonomicName id="4C214D51FFC3FFDCB196F88D827A446E" box="[698,842,1806,1831]" class="Myxosporea" family="Ceratomyxidae" genus="Ceratomyxa" kingdom="Animalia" order="Bivalvulida" pageId="7" pageNumber="164" phylum="Myxozoa" rank="species" species="herouardi">
|
||
<emphasis id="B955EAC0FFC3FFDCB196F88D827A446E" box="[698,842,1806,1831]" italics="true" pageId="7" pageNumber="164">C. herouardi</emphasis>
|
||
</taxonomicName>
|
||
based to observations in light microscopy and some erroneous hypothesis that attached to myxosporeans described many years ago. In recent paper, the different vegetative forms of
|
||
<taxonomicName id="4C214D51FFC3FFDCB25BF8D583354426" box="[375,517,1878,1903]" class="Myxosporea" family="Ceratomyxidae" genus="Ceratomyxa" kingdom="Animalia" order="Bivalvulida" pageId="7" pageNumber="164" phylum="Myxozoa" rank="species" species="herouardi">
|
||
<emphasis id="B955EAC0FFC3FFDCB25BF8D583354426" box="[375,517,1878,1903]" italics="true" pageId="7" pageNumber="164">C. herouardi</emphasis>
|
||
</taxonomicName>
|
||
are recognized and the first measurements of the sporogonic stages and as well as mature spore are given (see
|
||
<tableCitation id="C6A30369FFC3FFDCB2FEF8F8831644DA" box="[466,550,1914,1939]" captionStart="TABLE 5" captionStartId="22.[119,205,641,663]" captionText="TABLE 5. Comparisons between Mediterranean species of Ceratomyxa Thélohan, 1892 in fish of the family Sparidae. Measurements are given in µm as means ± standard deviation with range in parenthesis. SL: spore length; ST: spore thickness; PCL: polar capsule length; PCW: polar capsule width; PA: posterior angle; ND: not determined." pageId="7" pageNumber="164">Table 5</tableCitation>
|
||
).
|
||
</paragraph>
|
||
<paragraph id="8B9E36D2FFCCFFD3B3BBFF15806043E6" blockId="8.[151,1437,151,429]" box="[151,336,151,176]" pageId="8" pageNumber="165">Ecological notes</paragraph>
|
||
<paragraph id="8B9E36D2FFCCFFD3B3EBFF3E80F042E5" blockId="8.[151,1437,151,429]" pageId="8" pageNumber="165">
|
||
During the sampling period, the overall rate of infection is 28.2% and this myxosporean has a parasitic status as less frequent species even it has a great mean of intensity with 211.2 ± 50.6 spores per individual infected host (
|
||
<figureCitation id="131A2A57FFCCFFD3B3B3FE8681C44255" box="[159,244,260,285]" captionStart="FIGURE 10" captionStartId="25.[151,250,1431,1453]" captionTargetBox="[327,1259,891,1406]" captionTargetId="figure@25.[321,1266,888,1407]" captionTargetPageId="25" captionText="FIGURE 10. Mean intensity ± standard deviation of species of myxozoans infecting Sarpa Salpa." httpUri="https://zenodo.org/record/229280/files/figure.png" pageId="8" pageNumber="165">Fig. 10</figureCitation>
|
||
). Infection by
|
||
<taxonomicName id="4C214D51FFCCFFD3B2B2FE87831E4254" box="[414,558,260,285]" class="Myxosporea" family="Ceratomyxidae" genus="Ceratomyxa" kingdom="Animalia" order="Bivalvulida" pageId="8" pageNumber="165" phylum="Myxozoa" rank="species" species="herouardi">
|
||
<emphasis id="B955EAC0FFCCFFD3B2B2FE87831E4254" box="[414,558,260,285]" italics="true" pageId="8" pageNumber="165">C. herouardi</emphasis>
|
||
</taxonomicName>
|
||
was detected during the whole period of investigation. In Gulf of
|
||
<collectingCountry id="F3367642FFCCFFD3B607FE86845D4254" box="[1323,1389,260,285]" name="Tunisia" pageId="8" pageNumber="165">Tunis</collectingCountry>
|
||
, the infection started from March to August with maximum prevalence in June 46.7% while the infection commenced from March to June in Bay of Bizerte and maximum prevalence was recorded in May 26.7%. During examination of goldline sea bream’s gall bladder, the intensity of infection was important and showed no significant variation with months (see Table 4).
|
||
</paragraph>
|
||
</subSubSection>
|
||
</treatment>
|
||
</document> |