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<document id="47D2673B0E9981ECBF62038E6F127697" ID-DOI="10.11646/zootaxa.5533.1.1" ID-ISSN="1175-5334" ID-Zenodo-Dep="14023339" ID-ZooBank="F48DF7EE-7DED-49D3-96A5-620881E3AB36" IM.bibliography_approvedBy="felipe" IM.illustrations_approvedBy="felipe" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.tables_requiresApprovalFor="GgImagineBatch" IM.taxonomicNames_approvedBy="felipe" IM.treatments_approvedBy="felipe" checkinTime="1730454453128" checkinUser="plazi" docAuthor="Buhroo, Abdul Ahad" docDate="2024" docId="03DE8F5DFFA32D192DE9FA9E93384E51" docLanguage="en" docName="zootaxa.5533.1.1.pdf" docOrigin="Zootaxa 5533 (1)" docSource="http://dx.doi.org/10.11646/zootaxa.5533.1.1" docStyle="DocumentStyle:5EBBA59367AD13919D70D935FA04F6A3.19:Zootaxa.2013-.monograph" docStyleId="5EBBA59367AD13919D70D935FA04F6A3" docStyleName="Zootaxa.2013-.monograph" docStyleVersion="19" docTitle="Polygraphus trenchi Stebbing 1905" docType="treatment" docVersion="2" lastPageNumber="52" masterDocId="FFE7F725FF912D2A2D7EFFFF92344B25" masterDocTitle="A taxonomic monograph of subfamily Scolytinae (Coleoptera: Curculionidae) in the Western Himalaya" masterLastPageNumber="82" masterPageNumber="1" pageNumber="51" updateTime="1730455317287" updateUser="ExternalLinkService">
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<mods:title id="428FBD5C1A382F932FD86607884706BB">A taxonomic monograph of subfamily Scolytinae (Coleoptera: Curculionidae) in the Western Himalaya</mods:title>
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<mods:namePart id="59234A8D84A9F1965CD17D5D3E77FFCC">Buhroo, Abdul Ahad</mods:namePart>
<mods:nameIdentifier id="1B6FB19C251D9FF55691142014D86F2B" type="ORCID">0000-0002-9576-1165</mods:nameIdentifier>
<mods:affiliation id="0F932D073347CB727890B9CE89E02994">Section of Entomology, Department of Zoology, School of Biological Sciences, University of Kashmir, Srinagar- 190006 India abuhroo @ yahoo. com; https: // orcid. org / 0000 - 0002 - 9576 - 1165</mods:affiliation>
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<mods:title id="799F68E2C92D72AFA8AF036FE1CCB2C2">Zootaxa</mods:title>
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<mods:date id="B64D6E6A6181C8B93541E507AE48736B">2024</mods:date>
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<mods:identifier id="C7DD8D1B173441E0CACED09879393F45" type="DOI">10.11646/zootaxa.5533.1.1</mods:identifier>
<mods:identifier id="A31B6234A7739E39107FD98DD1647926" type="ISSN">1175-5334</mods:identifier>
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<treatment id="03DE8F5DFFA32D192DE9FA9E93384E51" LSID="urn:lsid:plazi:treatment:03DE8F5DFFA32D192DE9FA9E93384E51" httpUri="http://treatment.plazi.org/id/03DE8F5DFFA32D192DE9FA9E93384E51" lastPageId="51" lastPageNumber="52" pageId="50" pageNumber="51">
<subSubSection id="C36D6DC0FFA32D182DE9FA9E904E4E5E" box="[151,634,1376,1403]" pageId="50" pageNumber="51" type="nomenclature">
<paragraph id="8BC83E4BFFA32D182DE9FA9E904E4E5E" blockId="50.[151,634,1376,1440]" box="[151,634,1376,1403]" pageId="50" pageNumber="51">
<emphasis id="B903E259FFA32D182DE9FA9E904E4E5E" bold="true" box="[151,634,1376,1403]" pageId="50" pageNumber="51">
40.
<taxonomicName id="4C7745C8FFA32D182DBDFA9F904E4E5E" ID-CoL="77TZG" authority="Stebbing, 1905" authorityName="Stebbing" authorityYear="1905" box="[195,634,1376,1403]" class="Insecta" family="Curculionidae" genus="Polygraphus" kingdom="Animalia" order="Coleoptera" pageId="50" pageNumber="51" phylum="Arthropoda" rank="species" species="trenchi">
<emphasis id="B903E259FFA32D182DBDFA9F938E4E5E" bold="true" box="[195,442,1376,1403]" italics="true" pageId="50" pageNumber="51">Polygraphus trenchi</emphasis>
<bibRefCitation id="EFE643BAFFA32D182CBFFA9F904E4E5E" author="Stebbing, E. P." box="[449,634,1376,1403]" pageId="50" pageNumber="51" pagination="1 - 24" refId="ref61167" refString="Stebbing, E. P. (1905) A note on the Chilgoza bark boring beetles of Zhob, Baluchistan. Forest Bulletin, India, 3, 1 - 24." type="journal article" year="1905">Stebbing, 1905</bibRefCitation>
</taxonomicName>
</emphasis>
</paragraph>
</subSubSection>
<subSubSection id="C36D6DC0FFA32D192DE9FA7A93EE4859" lastPageId="51" lastPageNumber="52" pageId="50" pageNumber="51" type="description">
<paragraph id="8BC83E4BFFA32D182DE9FA7A93224E85" blockId="50.[151,634,1376,1440]" box="[151,278,1413,1440]" pageId="50" pageNumber="51">
(
<figureCitation id="134C22CEFFA32D182DE1FA7A933A4E85" box="[159,270,1413,1440]" captionStart="FIGURE 51" captionStartId="51.[151,250,723,747]" captionTargetBox="[391,1197,179,697]" captionTargetId="figure-17@51.[391,1197,179,697]" captionTargetPageId="51" captionText="FIGURE 51. Polygraphus trenchi. Male: dorsal view A, lateral view B; female: dorsal view C, lateral view D." figureDoi="http://doi.org/10.5281/zenodo.14023429" httpUri="https://zenodo.org/record/14023429/files/figure.png" pageId="50" pageNumber="51">Figure 51</figureCitation>
)
</paragraph>
<paragraph id="8BC83E4BFFA32D182DE9FA3290774CB2" blockId="50.[151,1437,1485,2015]" pageId="50" pageNumber="51">
This species is diagnosed by the following morphological characters:
<specimenCount id="9D71F5C2FFA32D182EC9FA3291F84EC2" box="[951,972,1485,1511]" count="1" pageId="50" pageNumber="51" type="female"></specimenCount>
frons plano-concave, surface shiny; two prominent, reddish-golden brushes of long hairs around the upper and lateral margins of frons, curled inwards and downwards without intersecting medially to about halfway and then continue in two thin strands, a few separate hairs covering the inner surface of eyes also meet the thin strands; antennal club ovate with pointed apex; pronotum 1.38× wider than long; lateral sides somewhat rounded on the basal half, narrowly constricted towards anterior third; a median conspicuous longitudinal raised line on pronotum, surface shining with minute punctures each with a fine hair, lateral margins set with moderate scales; scutellum depressed; elytra 1.43× as long as its width, twice as long as pronotum; basal margin crenulate, lateral sides somewhat parallel on basal two third, converging posteriorly into a rounded apex; striae narrow, marked with small round punctures on basal half of disc becoming much smaller posteriorly, striae 1 more impressed upto declivity; interstriae nearly four times wider than striae with close, fine granulations becoming larger and wider apart in apical fourth, interstrial surface with dense short scale-like setae; declivity convex with rows of minute interstrial granules; body moderately shining, dark brown; body length:
<quantity id="4C8F93AEFFA32D182DE9F88293084CB2" box="[151,316,1917,1944]" metricMagnitude="-3" metricUnit="m" metricValue="3.115" metricValueMax="3.15" metricValueMin="3.08" pageId="50" pageNumber="51" unit="mm" value="3.115" valueMax="3.15" valueMin="3.08">3.083.15 mm</quantity>
, 2.44× as long as wide.
</paragraph>
<paragraph id="8BC83E4BFFA32D182DB9F85E905F4CFA" blockId="50.[151,1437,1485,2015]" pageId="50" pageNumber="51">
Males are smaller than females; frons has few sparse yellow hairs and two small, median tubercles, placed transversely; body length:
<quantity id="4C8F93AEFFA32D182CBFF83A90534CFA" box="[449,615,1989,2015]" metricMagnitude="-3" metricUnit="m" metricValue="2.935" metricValueMax="2.97" metricValueMin="2.9" pageId="50" pageNumber="51" unit="mm" value="2.935" valueMax="2.97" valueMin="2.9">2.902.97 mm</quantity>
.
</paragraph>
<caption id="DF086EC3FFA22D192DE9FD2C96CE49CE" ID-DOI="http://doi.org/10.5281/zenodo.14023429" ID-Zenodo-Dep="14023429" box="[151,1274,723,748]" httpUri="https://zenodo.org/record/14023429/files/figure.png" pageId="51" pageNumber="52" startId="51.[151,250,723,747]" targetBox="[391,1197,179,697]" targetPageId="51" targetType="figure">
<paragraph id="8BC83E4BFFA22D192DE9FD2C96CE49CE" blockId="51.[151,1274,723,748]" box="[151,1274,723,748]" pageId="51" pageNumber="52">
<emphasis id="B903E259FFA22D192DE9FD2C931449CE" bold="true" box="[151,288,723,747]" pageId="51" pageNumber="52">FIGURE 51.</emphasis>
<taxonomicName id="4C7745C8FFA22D192C58FD2C93C149CE" authorityName="Stebbing" authorityYear="1905" box="[294,501,723,747]" class="Insecta" family="Curculionidae" genus="Polygraphus" kingdom="Animalia" order="Coleoptera" pageId="51" pageNumber="52" phylum="Arthropoda" rank="species" species="trenchi">
<emphasis id="B903E259FFA22D192C58FD2C93C149CE" box="[294,501,723,747]" italics="true" pageId="51" pageNumber="52">Polygraphus trenchi</emphasis>
</taxonomicName>
. Male: dorsal view
<emphasis id="B903E259FFA22D192FC3FD2C90FB49CE" bold="true" box="[701,719,723,747]" pageId="51" pageNumber="52">A</emphasis>
, lateral view
<emphasis id="B903E259FFA22D192E27FD2B915E49C9" bold="true" box="[857,874,724,748]" pageId="51" pageNumber="52">B</emphasis>
; female: dorsal view
<emphasis id="B903E259FFA22D192938FD2C966C49CE" bold="true" box="[1094,1112,723,747]" pageId="51" pageNumber="52">C</emphasis>
, lateral view
<emphasis id="B903E259FFA22D19299CFD2B96C049C9" bold="true" box="[1250,1268,724,748]" pageId="51" pageNumber="52">D</emphasis>
.
</paragraph>
</caption>
<paragraph id="8BC83E4BFFA22D192DB9FCC193EE4859" blockId="51.[151,1437,830,1072]" pageId="51" pageNumber="52">Remarks: The position and configuration of frontal brushes in females serve as a diagnostic feature in the identification of this species.</paragraph>
</subSubSection>
<subSubSection id="C36D6DC0FFA22D192DB9FC7A90CE48CD" pageId="51" pageNumber="52" type="materials_examined">
<paragraph id="8BC83E4BFFA22D192DB9FC7A90CE48CD" blockId="51.[151,1437,830,1072]" pageId="51" pageNumber="52">
<emphasis id="B903E259FFA22D192DB9FC7A938248BA" bold="true" box="[199,438,901,927]" pageId="51" pageNumber="52">Material examined:</emphasis>
<materialsCitation id="3B1F3416FFA22D192CBEFC7990C248CD" collectingDate="2017-09-09" collectionCode="KUIC" collectorName="A. A. Buhroo &amp; Jammu &amp; Kishtwar &amp; Kijaye Padder" country="India" county="Kundhal Padder" elevation="2134" latitude="33.269066" location="New" longLatPrecision="1" longitude="76.128" municipality="Kishtwar" pageId="51" pageNumber="52" specimenCount="4" specimenCount-female="2" specimenCount-male="2">
<location id="8EA86890FFA22D192CBEFC7993C14885" LSID="urn:lsid:plazi:treatment:03DE8F5DFFA32D192DE9FA9E93384E51:8EA86890FFA22D192CBEFC7993C14885" box="[448,501,902,928]" country="India" county="Kundhal Padder" latitude="33.269066" longLatPrecision="1" longitude="76.128" municipality="Kishtwar" name="New" pageId="51" pageNumber="52">New</location>
records:
<collectingCountry id="F3607EDBFFA22D192F1DFC7990954885" box="[611,673,902,928]" name="India" pageId="51" pageNumber="52">India</collectingCountry>
:
<specimenCount id="9D71F5C2FFA22D192FD1FC7990E94887" box="[687,733,902,930]" count="2" pageId="51" pageNumber="52" type="male">2 ♂</specimenCount>
s,
<specimenCount id="9D71F5C2FFA22D192F80FC79911D4887" box="[766,809,902,930]" count="2" pageId="51" pageNumber="52" type="female">2 ♀</specimenCount>
s.
<location id="8EA86890FFA22D192E30FC7991964885" LSID="urn:lsid:plazi:treatment:03DE8F5DFFA32D192DE9FA9E93384E51:8EA86890FFA22D192E30FC7991964885" box="[846,930,902,928]" country="India" county="Kundhal Padder" latitude="33.269066" longLatPrecision="1" longitude="76.128" municipality="Kishtwar" name="Jammu" pageId="51" pageNumber="52">Jammu</location>
,
<collectingMunicipality id="6BACA431FFA22D192ECEFC79962C4885" box="[944,1048,902,928]" pageId="51" pageNumber="52">Kishtwar</collectingMunicipality>
,
<collectingCounty id="62A946C7FFA22D192958FC7996EB4885" box="[1062,1247,902,928]" pageId="51" pageNumber="52">Kundhal Padder</collectingCounty>
(
<geoCoordinate id="EE43588CFFA22D19298CFC7997AC4885" box="[1266,1432,902,930]" degrees="33" direction="north" minutes="18.820" orientation="latitude" pageId="51" pageNumber="52" precision="1" value="33.313667">33° 18.820 N</geoCoordinate>
,
<geoCoordinate id="EE43588CFFA22D192DE9FC56930B48E1" box="[151,319,937,966]" degrees="076" direction="east" minutes="04.492" orientation="longitude" pageId="51" pageNumber="52" precision="1" value="76.07487">076° 04.492 E</geoCoordinate>
,
<quantity id="4C8F93AEFFA22D192C35FC55939048E1" box="[331,420,938,964]" metricMagnitude="3" metricUnit="m" metricValue="2.1336" pageId="51" pageNumber="52" unit="ft" value="7000.0">
<elevation id="005AD978FFA22D192C35FC55939048E1" box="[331,420,938,964]" metricMagnitude="3" metricUnit="m" metricValue="2.1336" pageId="51" pageNumber="52" unit="ft" value="7000.0">7000 ft.</elevation>
</quantity>
),
<collectorName id="26825B9DFFA22D192CC9FC55907F48E1" box="[439,587,938,964]" pageId="51" pageNumber="52">A.A. Buhroo</collectorName>
,
<date id="FFC9188BFFA22D192F2BFC5590E748E1" box="[597,723,938,964]" pageId="51" pageNumber="52" value="2017-09-09">
<collectingDate id="EF8DE163FFA22D192F2BFC5590E748E1" box="[597,723,938,964]" pageId="51" pageNumber="52" value="2017-09-09">09.09.2017</collectingDate>
</date>
(
<collectionCode id="ED66A68EFFA22D192F9CFC55911948E1" box="[738,813,938,964]" country="Japan" httpUri="http://grbio.org/cool/nivr-mmiq" name="Kagoshima University" pageId="51" pageNumber="52">KUIC</collectionCode>
).
<collectorName id="26825B9DFFA22D192E40FC5591A648E1" box="[830,914,938,964]" pageId="51" pageNumber="52">Jammu</collectorName>
,
<collectorName id="26825B9DFFA22D192EE2FC55963048E1" box="[924,1028,938,964]" pageId="51" pageNumber="52">Kishtwar</collectorName>
,
<collectorName id="26825B9DFFA22D192971FC55969B48E1" box="[1039,1199,938,964]" pageId="51" pageNumber="52">Kijaye Padder</collectorName>
(
<geoCoordinate id="EE43588CFFA22D1929C3FC55976848E1" box="[1213,1372,938,966]" degrees="33" direction="north" minutes="16.144" orientation="latitude" pageId="51" pageNumber="52" precision="1" value="33.269066">33° 16.144 N</geoCoordinate>
,
<geoCoordinate id="EE43588CFFA22D192819FC5692CA48CD" degrees="076" direction="east" minutes="07.680" orientation="longitude" pageId="51" pageNumber="52" precision="1" value="76.128">076° 07.680 E</geoCoordinate>
,
<quantity id="4C8F93AEFFA22D192C74FC32935048CD" box="[266,356,973,1000]" metricMagnitude="3" metricUnit="m" metricValue="1.8729959999999999" pageId="51" pageNumber="52" unit="ft" value="6145.0">
<elevation id="005AD978FFA22D192C74FC32935048CD" box="[266,356,973,1000]" metricMagnitude="3" metricUnit="m" metricValue="1.8729959999999999" pageId="51" pageNumber="52" unit="ft" value="6145.0">6145 ft.</elevation>
</quantity>
),
<collectorName id="26825B9DFFA22D192C09FC31903848CD" box="[375,524,974,1000]" pageId="51" pageNumber="52">A.A. Buhroo</collectorName>
,
<date id="FFC9188BFFA22D192F69FC3190A048CD" box="[535,660,974,1000]" pageId="51" pageNumber="52" value="2017-09-09">
<collectingDate id="EF8DE163FFA22D192F69FC3190A048CD" box="[535,660,974,1000]" pageId="51" pageNumber="52" value="2017-09-09">09.09.2017</collectingDate>
</date>
(
<collectionCode id="ED66A68EFFA22D192FDAFC3190DB48CD" box="[676,751,974,1000]" country="Japan" httpUri="http://grbio.org/cool/nivr-mmiq" name="Kagoshima University" pageId="51" pageNumber="52">KUIC</collectionCode>
)
</materialsCitation>
.
</paragraph>
</subSubSection>
<subSubSection id="C36D6DC0FFA22D192DB9FC0E91574F29" box="[199,867,1009,1036]" pageId="51" pageNumber="52" type="distribution">
<paragraph id="8BC83E4BFFA22D192DB9FC0E91574F29" blockId="51.[151,1437,830,1072]" box="[199,867,1009,1036]" pageId="51" pageNumber="52">
<emphasis id="B903E259FFA22D192DB9FC0E93574F2E" bold="true" box="[199,355,1009,1035]" pageId="51" pageNumber="52">Distribution:</emphasis>
<collectingCountry id="F3607EDBFFA22D192C14FC0D939C4F29" box="[362,424,1010,1036]" name="India" pageId="51" pageNumber="52">India</collectingCountry>
: Jammu (new record),
<collectingRegion id="49B3F0A9FFA22D192FD6FC0D90CD4F29" box="[680,761,1010,1036]" country="Pakistan" name="Punjab" pageId="51" pageNumber="52">Punjab</collectingRegion>
,
<collectingCountry id="F3607EDBFFA22D192E7AFC0D91574F29" box="[772,867,1010,1036]" name="Pakistan" pageId="51" pageNumber="52">Pakistan</collectingCountry>
</paragraph>
</subSubSection>
<subSubSection id="C36D6DC0FFA22D192DB9FBE993384E51" pageId="51" pageNumber="52" type="biology_ecology">
<paragraph id="8BC83E4BFFA22D192DB9FBE990684F15" blockId="51.[151,1437,830,1072]" box="[199,604,1046,1072]" pageId="51" pageNumber="52">
<emphasis id="B903E259FFA22D192DB9FBE993274F15" bold="true" box="[199,275,1046,1072]" pageId="51" pageNumber="52">Hosts:</emphasis>
<taxonomicName id="4C7745C8FFA22D192C64FBE993EA4F15" box="[282,478,1046,1072]" class="Pinopsida" family="Pinaceae" genus="Pinus" kingdom="Plantae" order="Pinales" pageId="51" pageNumber="52" phylum="Tracheophyta" rank="species" species="gerardiana">
<emphasis id="B903E259FFA22D192C64FBE993EA4F15" box="[282,478,1046,1072]" italics="true" pageId="51" pageNumber="52">Pinus gerardiana</emphasis>
</taxonomicName>
(
<taxonomicName id="4C7745C8FFA22D192C93FBE990604F15" box="[493,596,1046,1072]" class="Pinopsida" family="Pinaceae" kingdom="Plantae" order="Pinales" pageId="51" pageNumber="52" phylum="Tracheophyta" rank="family">Pinaceae</taxonomicName>
)
</paragraph>
<paragraph id="8BC83E4BFFA22D192DE9FBA293F54F5D" blockId="51.[151,1437,1117,1396]" box="[151,449,1117,1144]" pageId="51" pageNumber="52">
<emphasis id="B903E259FFA22D192DE9FBA2938E4F5D" bold="true" box="[151,442,1117,1144]" pageId="51" pageNumber="52">Phylogenetic assessment</emphasis>
:
</paragraph>
<paragraph id="8BC83E4BFFA22D192DE9FB7D93384E51" blockId="51.[151,1437,1117,1396]" pageId="51" pageNumber="52">
The phylogenetic treatment of the Himalayan
<taxonomicName id="4C7745C8FFA22D192FE5FB7D911E4FB9" authorityName="Erichson" authorityYear="1836" box="[667,810,1154,1180]" class="Insecta" family="Curculionidae" genus="Polygraphus" kingdom="Animalia" order="Coleoptera" pageId="51" pageNumber="52" phylum="Arthropoda" rank="genus">
<emphasis id="B903E259FFA22D192FE5FB7D911E4FB9" box="[667,810,1154,1180]" italics="true" pageId="51" pageNumber="52">Polygraphus</emphasis>
</taxonomicName>
along with other closely related species was carried out using the COX-1 genes to examine the monophyly and sister groups of each species (
<figureCitation id="134C22CEFFA22D19292BFB59969E4FE5" box="[1109,1194,1189,1216]" captionStart="FIGURE 52" captionStartId="51.[151,250,1841,1865]" captionTargetBox="[529,1060,1422,1810]" captionTargetId="figure-222@51.[523,1063,1417,1815]" captionTargetPageId="51" captionText="FIGURE 52. A phylogenetic tree obtained from the NJ for the COX-1 genes of Himalayan Polygraphus (in blue) and related species. Numbers at the branches represent bootstrap values." figureDoi="http://doi.org/10.5281/zenodo.14023431" httpUri="https://zenodo.org/record/14023431/files/figure.png" pageId="51" pageNumber="52">Fig. 52</figureCitation>
). The NJ tree showed that
<taxonomicName id="4C7745C8FFA22D192DB4FB3593024FC1" authorityName="Stebbing" authorityYear="1905" box="[202,310,1226,1252]" class="Insecta" family="Curculionidae" genus="Polygraphus" kingdom="Animalia" order="Coleoptera" pageId="51" pageNumber="52" phylum="Arthropoda" rank="species" species="trenchi">
<emphasis id="B903E259FFA22D192DB4FB3593024FC1" box="[202,310,1226,1252]" italics="true" pageId="51" pageNumber="52">P. trenchi</emphasis>
</taxonomicName>
populations are monophyletic with
<taxonomicName id="4C7745C8FFA22D192FABFB3596294FC1" authority="Blandford, 1894" authorityName="Blandford" authorityYear="1894" box="[725,1053,1226,1252]" class="Insecta" family="Curculionidae" genus="Polygraphus" kingdom="Animalia" order="Coleoptera" pageId="51" pageNumber="52" phylum="Arthropoda" rank="species" species="proximus">
<emphasis id="B903E259FFA22D192FABFB35916E4FC6" box="[725,858,1226,1251]" italics="true" pageId="51" pageNumber="52">P. proximus</emphasis>
<bibRefCitation id="EFE643BAFFA22D192E1DFB3596294FC1" author="Blandford, W. F. H." box="[867,1053,1226,1252]" pageId="51" pageNumber="52" pagination="53 - 141" refId="ref55923" refString="Blandford, W. F. H. (1894) The rhynchophorous Coleoptera of Japan, Part III. Scolytidae. Transactions of the Entomological Society of London, 42 (1), 53 - 141. https: // doi. org / 10.1111 / j. 1365 - 2311.1894. tb 02347. x" type="journal article" year="1894">Blandford, 1894</bibRefCitation>
</taxonomicName>
populations distributed in
<collectingCountry id="F3607EDBFFA22D19282CFB3597A94FC1" box="[1362,1437,1226,1252]" name="Russia" pageId="51" pageNumber="52">Russia</collectingCountry>
and that they differ from them by 14.7314.89%, while
<taxonomicName id="4C7745C8FFA22D192E74FB1191664E22" authorityName="Stebbing" authorityYear="1914" box="[778,850,1262,1287]" class="Insecta" family="Curculionidae" genus="Polygraphus" kingdom="Animalia" order="Coleoptera" pageId="51" pageNumber="52" phylum="Arthropoda" rank="species" species="pini">
<emphasis id="B903E259FFA22D192E74FB1191664E22" box="[778,850,1262,1287]" italics="true" pageId="51" pageNumber="52">P. pini</emphasis>
</taxonomicName>
populations formed a separate monophyletic cluster with well-supported bootstrap values and that their intraspecific nucleotide difference was between 0.51.92%. The research also revealed that
<taxonomicName id="4C7745C8FFA22D192CB1FAC9900E4E75" authorityName="Stebbing" authorityYear="1905" box="[463,570,1334,1360]" class="Insecta" family="Curculionidae" genus="Polygraphus" kingdom="Animalia" order="Coleoptera" pageId="51" pageNumber="52" phylum="Arthropoda" rank="species" species="trenchi">
<emphasis id="B903E259FFA22D192CB1FAC9900E4E75" box="[463,570,1334,1360]" italics="true" pageId="51" pageNumber="52">P. trenchi</emphasis>
</taxonomicName>
and
<taxonomicName id="4C7745C8FFA22D192F0BFAC990E24E6A" authorityName="Stebbing" authorityYear="1903" box="[629,726,1334,1359]" class="Insecta" family="Curculionidae" genus="Polygraphus" kingdom="Animalia" order="Coleoptera" pageId="51" pageNumber="52" phylum="Arthropoda" rank="species" species="major">
<emphasis id="B903E259FFA22D192F0BFAC990E24E6A" box="[629,726,1334,1359]" italics="true" pageId="51" pageNumber="52">P. major</emphasis>
</taxonomicName>
differed by 14.07%, whilst
<taxonomicName id="4C7745C8FFA22D192967FAC9964E4E6A" authorityName="Stebbing" authorityYear="1903" box="[1049,1146,1334,1359]" class="Insecta" family="Curculionidae" genus="Polygraphus" kingdom="Animalia" order="Coleoptera" pageId="51" pageNumber="52" phylum="Arthropoda" rank="species" species="major">
<emphasis id="B903E259FFA22D192967FAC9964E4E6A" box="[1049,1146,1334,1359]" italics="true" pageId="51" pageNumber="52">P. major</emphasis>
</taxonomicName>
and
<taxonomicName id="4C7745C8FFA22D1929CAFAC996CA4E6A" authorityName="Stebbing" authorityYear="1914" box="[1204,1278,1334,1359]" class="Insecta" family="Curculionidae" genus="Polygraphus" kingdom="Animalia" order="Coleoptera" pageId="51" pageNumber="52" phylum="Arthropoda" rank="species" species="pini">
<emphasis id="B903E259FFA22D1929CAFAC996CA4E6A" box="[1204,1278,1334,1359]" italics="true" pageId="51" pageNumber="52">P. pini</emphasis>
</taxonomicName>
were 15.37% dissimilar.
</paragraph>
</subSubSection>
</treatment>
</document>