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<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.102.113696" ID-Pensoft-Pub="1314-4049-102-201" ID-Pensoft-UUID="86674352E59B513ABE2F03A64B2F2AEB" ModsDocID="1314-4049-102-201" checkinTime="1709112337990" checkinUser="pensoft" docAuthor="Wang, Yifeng, Tsui, Kin-Ming, Chen, Shimei &amp; You, Chongjuan" docDate="2024" docId="CEDB10C56EA75A6BB6E0339FC755F012" docLanguage="en" docName="MycoKeys 102: 201-224" docOrigin="MycoKeys 102" docPubDate="2024-02-27" docSource="http://dx.doi.org/10.3897/mycokeys.102.113696" docTitle="Neopestalotiopsis sp. 4" docType="treatment" docVersion="1" id="86674352E59B513ABE2F03A64B2F2AEB" lastPageNumber="201" masterDocId="86674352E59B513ABE2F03A64B2F2AEB" masterDocTitle="Diversity, pathogenicity and two new species of pestalotioid fungi (Amphisphaeriales) associated with Chinese Yew in Guangxi, China" masterLastPageNumber="224" masterPageNumber="201" pageNumber="201" updateTime="1709112337990" updateUser="pensoft">
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<mods:title id="485CE56AC7C638480FBAAC3C3D2F0808">Diversity, pathogenicity and two new species of pestalotioid fungi (Amphisphaeriales) associated with Chinese Yew in Guangxi, China</mods:title>
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<mods:namePart id="877119A472A1ECDC1795C2B3D405A912">Wang, Yifeng</mods:namePart>
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<mods:affiliation id="BBBF32B56B6EBE03FB2B4E4F95737A0A">Beijing Key Laboratory for Forest Pest Control, Beijing Forestry University, Beijing 100083, China</mods:affiliation>
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<mods:namePart id="183C48F0ECFB5C461368BE429013EB7C">Tsui, Kin-Ming</mods:namePart>
<mods:nameIdentifier id="E26818243B836C3F0FC18785E9CE4CB8" type="ORCID">https://orcid.org/0000-0001-5129-1037</mods:nameIdentifier>
<mods:affiliation id="31DD84EFCBEE18A10262F411E71B33AB">National Centre for Infectious Diseases, Tan Tock Seng Hospital, Singapore 308433, Singapore &amp; Faculty of Medicine, University of British Columbia, Vancouver, V 6 T 1 Z 3, Canada</mods:affiliation>
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<mods:namePart id="0F031C17775A0E390204CA1784061E89">Chen, Shimei</mods:namePart>
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<mods:affiliation id="24487F0D378B19AB2419C405BFB81017">Beijing Key Laboratory for Forest Pest Control, Beijing Forestry University, Beijing 100083, China</mods:affiliation>
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<mods:namePart id="9729508CF73CF53722242DBC2BC23939">You, Chongjuan</mods:namePart>
<mods:nameIdentifier id="3C1B7FE321A02BC4C3A3EE8104481389" type="ORCID">https://orcid.org/0000-0001-6130-5703</mods:nameIdentifier>
<mods:affiliation id="AFD881C76E53079C66DA1DB567BD0251">Beijing Key Laboratory for Forest Pest Control, Beijing Forestry University, Beijing 100083, China</mods:affiliation>
<mods:nameIdentifier id="DB182BA964BF411C67097E532A18F7E6" type="email">chongjuanyou@bjfu.edu.cn</mods:nameIdentifier>
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<treatment id="CEDB10C56EA75A6BB6E0339FC755F012" LSID="urn:lsid:plazi:treatment:CEDB10C56EA75A6BB6E0339FC755F012" httpUri="http://treatment.plazi.org/id/CEDB10C56EA75A6BB6E0339FC755F012" lastPageNumber="201" pageId="0" pageNumber="201">
<subSubSection id="0C34009F2E599AAAF8E63EFCF7CFAED7" pageId="0" pageNumber="201" type="nomenclature">
<paragraph id="5ADC641023C419845B66D0145680A401" pageId="0" pageNumber="201">
<taxonomicName id="B632E3F4DF4873A134FB75A9D3714BFE" LSID="CEDB10C5-6EA7-5A6B-B6E0-339FC755F012" authority="sp. 4" authorityName="sp. 4" class="Sordariomycetes" family="Sporocadaceae" genus="Neopestalotiopsis" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Neopestalotiopsis" order="Amphisphaeriales" pageId="0" pageNumber="201" phylum="Ascomycota" rank="genus">Neopestalotiopsis sp. 4</taxonomicName>
</paragraph>
</subSubSection>
<subSubSection id="AEC74AEC72E6AC340455880C2A9ECC64" pageId="0" pageNumber="201" type="description">
<paragraph id="1CAA1A511E7BD824F4377F70A4E7884E" pageId="0" pageNumber="201">
<figureCitation id="F07BD5999016F02C65EFB0A19A61F70C" captionStart="Figure 8" captionStartId="F8" captionText="Figure 8. Neopestalotiopsis sp. 4 (CFCC 59989) a culture on PDA b conidiomata form on PDA c-e conidia. Scale bars: 500 µm (b); 10 µm (c-e)." figureDoi="10.3897/mycokeys.102.113696.figure8" httpUri="https://binary.pensoft.net/fig/993284" pageId="0" pageNumber="201">Fig. 8</figureCitation>
</paragraph>
</subSubSection>
<subSubSection id="4ED25D880801AB84F2B17CB87C130B63" pageId="0" pageNumber="201" type="conidiogenesis">
<paragraph id="2AA1B5D50D6D5F0D07BDDC4CC01357DD" pageId="0" pageNumber="201">Conidiogenesis.</paragraph>
<paragraph id="3377F82FB5E8075D9A38EDC6774F8B7B" pageId="0" pageNumber="201">
Conidiophores reduced to conidiogenous cells, hyaline, smooth. Conidiogenous cells were discrete, ampulliform, thin-walled, hyaline to light brown, smooth. Conidia fusiform to clavate, straight or slightly curved, olivaceous to brown, 4-septate, 15.5-18.5
<normalizedToken id="4C716B14A00725DD3EC32E927B34F224" originalValue="×">x</normalizedToken>
6-8
<normalizedToken id="D6C73E99E5D5841BE9799610A938556F" originalValue="μm">μm</normalizedToken>
, with apical and basal appendages. Basal cell obconic, hyaline, thin-walled, smooth, 2.5-4
<normalizedToken id="D4C2767561D976627F322AEC8EFB5EE2" originalValue="µm">µm</normalizedToken>
; the three median cells 10.5-12
<normalizedToken id="26849EB9A2849F841EFEF591BB8AB4A5" originalValue="µm">µm</normalizedToken>
, dolioform, versicolor, pale brown to brown with septa darker than the rest of the cells, the second cell from base 3-3.5
<normalizedToken id="9B2DADDAF374D9341A56BC1039D54438" originalValue="µm">µm</normalizedToken>
; the third cell 3-4.5
<normalizedToken id="489AA1D1754C0D3B067B964270F52DCD" originalValue="µm">µm</normalizedToken>
; the fourth cell 3-5.5
<normalizedToken id="52472FB25D8DF23ECD7E9A6DD4D63724" originalValue="µm">µm</normalizedToken>
; apical cell 1-2.5
<normalizedToken id="3656B6B93BC82D3212E79A2C96ABFE90" originalValue="µm">µm</normalizedToken>
, cylindrical, hyaline; 3-4 (mostly 3) tubular apical appendages, arising from the apex of the apical cell each at a different point, filiform, 9.5-15.5
<normalizedToken id="E3D3FF19C79FA1DFF06272684ECCC430" originalValue="µm">µm</normalizedToken>
; basal appendage present most of the time, single, tubular, unbranched, 1.5-4.5
<normalizedToken id="51C54C18E957EDF586F324AC6BE5A48C" originalValue="µm">µm</normalizedToken>
(Fig.
<figureCitation id="BC625CF25E908078AF843A3FEAABB520" captionStart="Figure 8" captionStartId="F8" captionText="Figure 8. Neopestalotiopsis sp. 4 (CFCC 59989) a culture on PDA b conidiomata form on PDA c-e conidia. Scale bars: 500 µm (b); 10 µm (c-e)." figureDoi="10.3897/mycokeys.102.113696.figure8" httpUri="https://binary.pensoft.net/fig/993284" pageId="0" pageNumber="201">8c-e</figureCitation>
). Sexual morph not observed.
</paragraph>
<caption id="D04A875AB898047C37DA2790141CB78C" doi="10.3897/mycokeys.102.113696.figure8" httpUri="https://binary.pensoft.net/fig/993284" pageId="0" pageNumber="201" start="Figure 8" startId="F8">
<paragraph id="8E7A85CDE6D3E255FABD030BECD5B933" pageId="0" pageNumber="201">
<emphasis id="8DA67D1843A70875D4F75D80DC3F60EA" bold="true" pageId="0" pageNumber="201">Figure 8.</emphasis>
<taxonomicName id="77F07C200B2A639F8C4FC4C00EC992B7" class="Sordariomycetes" family="Sporocadaceae" genus="Neopestalotiopsis" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Neopestalotiopsis" order="Amphisphaeriales" pageId="0" pageNumber="201" phylum="Ascomycota" rank="genus">
<emphasis id="A7889AA2C83F63C23AA6D0E90A46286A" italics="true" pageId="0" pageNumber="201">Neopestalotiopsis</emphasis>
</taxonomicName>
sp. 4 (CFCC59989)
<emphasis id="9AA28D7BE2DC92C554F3656FA1B7825F" bold="true" pageId="0" pageNumber="201">a</emphasis>
culture on PDA
<emphasis id="52C046A9670EC032B985F3A1F67529F4" bold="true" pageId="0" pageNumber="201">b</emphasis>
conidiomata form on PDA
<emphasis id="0F806C3BD27900F74726C5A041A6905F" bold="true" pageId="0" pageNumber="201">c-e</emphasis>
conidia. Scale bars: 500
<normalizedToken id="0AA5F042650A23AD2190CB1D0D918D0C" originalValue="µm">µm</normalizedToken>
(
<emphasis id="AA2DE21D5AB284FFF220326747FC84AD" bold="true" pageId="0" pageNumber="201">b</emphasis>
); 10
<normalizedToken id="243AE1C0CEABD9F0AE317BD7D2C80816" originalValue="µm">µm</normalizedToken>
(
<emphasis id="A4FDDB895B1A194530DBD42917BFE90C" bold="true" pageId="0" pageNumber="201">c-e</emphasis>
).
</paragraph>
</caption>
</subSubSection>
<subSubSection id="7E77F05D4770135A87B8BFEDC7EA4B70" pageId="0" pageNumber="201" type="description">
<paragraph id="716E66FCDAF674703E95B7ECA9CE66BE" pageId="0" pageNumber="201">Culture characteristics.</paragraph>
<paragraph id="3BBE460EB69491420B4196B185D21575" pageId="0" pageNumber="201">
Colonies on PDA reaching 90 mm diameter after seven days at 25 °C, radiating outwards with an undulate and radial edge, white aerial mycelium was flat on the centres of the colony, while raised sharply in the outer ring (Fig.
<figureCitation id="DA4120A0AED31D76D47F44142E3DA420" captionStart="Figure 8" captionStartId="F8" captionText="Figure 8. Neopestalotiopsis sp. 4 (CFCC 59989) a culture on PDA b conidiomata form on PDA c-e conidia. Scale bars: 500 µm (b); 10 µm (c-e)." figureDoi="10.3897/mycokeys.102.113696.figure8" httpUri="https://binary.pensoft.net/fig/993284" pageId="0" pageNumber="201">8a</figureCitation>
).
<emphasis id="C4A7967DA09CF256D156C0856D293EF8" italics="true" pageId="0" pageNumber="201">Conidiomata acervular</emphasis>
in culture on PDA, globose, 100-900
<normalizedToken id="680AC4143361917C229A5B930EC23BB5" originalValue="μm">μm</normalizedToken>
diameter, solitary or aggregated in clusters, exuding black conidial masses (Fig.
<figureCitation id="183431B1C21149EF30D8364714F66B80" captionStart="Figure 8" captionStartId="F8" captionText="Figure 8. Neopestalotiopsis sp. 4 (CFCC 59989) a culture on PDA b conidiomata form on PDA c-e conidia. Scale bars: 500 µm (b); 10 µm (c-e)." figureDoi="10.3897/mycokeys.102.113696.figure8" httpUri="https://binary.pensoft.net/fig/993284" pageId="0" pageNumber="201">8b</figureCitation>
).
</paragraph>
</subSubSection>
<subSubSection id="4C7AA19E0AF270FE26A08B97C4F61C03" pageId="0" pageNumber="201" type="materials_examined">
<paragraph id="819E530F7E5B233BB09CA3CBC61EF043" pageId="0" pageNumber="201">Material examined.</paragraph>
<paragraph id="33C50C31D5D4DEE2AB0058F73DE8A3C2" pageId="0" pageNumber="201">
<materialsCitation id="027D55E98BB06C024591487153F32FDA" collectingDate="2020-05" collectorName="Y. F. Wang" country="China" location="Gungxi Province" municipality="Chinese" specimenCount="1">
<collectingCountry id="CB670AC4624DFDA0CADB0F533A9D7C52" name="China">China</collectingCountry>
,
<location id="9E0D64531FB74FA30DF68205A02BC967" LSID="urn:lsid:plazi:treatment:CEDB10C56EA75A6BB6E0339FC755F012:9E0D64531FB74FA30DF68205A02BC967" country="China" municipality="Chinese" name="Gungxi Province">Gungxi Province</location>
, from diseased branches of
<collectingMunicipality id="F9955955331A813D1BB1F38034EE99DC">Chinese</collectingMunicipality>
yew,
<collectingDate id="0625FB64DCE5390340E907F543B96D18" value="2020-05">May 2020</collectingDate>
,
<collectorName id="1F540B0CA9FC83EEE1807192D8909125">Y. F. Wang</collectorName>
(BJFC-S1958); living cultures CFCC59989, CFCC59990, CFCC59991 and CFCC59992
</materialsCitation>
.
</paragraph>
</subSubSection>
<subSubSection id="23AA825F4BFE7D8ACF683A9FD7C77F66" pageId="0" pageNumber="201" type="notes">
<paragraph id="9080D7220B5A49BA0C7D4983EFCEE172" pageId="0" pageNumber="201">Notes.</paragraph>
<paragraph id="73A288E413E699B43BB43B8A3B71940F" pageId="0" pageNumber="201">
<taxonomicName id="213C290D74354EE298455219247F7D3F" class="Sordariomycetes" family="Sporocadaceae" genus="Neopestalotiopsis" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Neopestalotiopsis" order="Amphisphaeriales" pageId="0" pageNumber="201" phylum="Ascomycota" rank="genus">
<emphasis id="4AB6AB1C40A3B1739AF84E56E774B7DD" italics="true" pageId="0" pageNumber="201">Neopestalotiopsis</emphasis>
</taxonomicName>
sp. 4 (CFCC59989 and CFCC59990) was phylogenetically placed in a clade encompassing two
<taxonomicName id="D5C1D262442318581331ECE2320F6EEA" class="Sordariomycetes" family="Sporocadaceae" genus="Neopestalotiopsis" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Neopestalotiopsis" order="Amphisphaeriales" pageId="0" pageNumber="201" phylum="Ascomycota" rank="genus">
<emphasis id="52E607B3988E3916C3E0AF97A88A0189" italics="true" pageId="0" pageNumber="201">Neopestalotiopsis</emphasis>
</taxonomicName>
sp. 2 isolates (CFCC54340 and ZX22B) from Yaan City, Sichuan Province (
<bibRefCitation id="A640E4B7703FB02D05ECA1E29DCE7284" DOI="https://doi.org/10.3390/jof7010064" author="Jiang, N" journalOrPublisher="Journal of Fungi" pageId="0" pageNumber="201" pagination=": 64" refId="B15" refString="Jiang, N, Fan, X, Tian, C, 2021. Identification and characterization of leaf-Inhabiting fungi from Castanea plantations in China. Journal of Fungi 7 (1): 64, DOI: https://doi.org/10.3390/jof7010064" title="Identification and characterization of leaf-Inhabiting fungi from Castanea plantations in China." url="https://doi.org/10.3390/jof7010064" volume="7" year="2021">Jiang et al. 2021</bibRefCitation>
) (Fig.
<figureCitation id="05C20162F2535589919E7FAD622ABC9C" captionStart="Figure 3" captionStartId="F3" captionText="Figure 3. A phylogenetic tree of Neopestalotiopsis generated from MP analysis, based on combined ITS, tub 2 and tef- 1 α sequence data. Maximum Parsimony and Maximum Likelihood bootstrap values ≥ 65 % are given at the nodes. The branches with Bayesian Inference posterior probabilities ≥ 0.90 are bold. Strains from this study are marked in blue. Ex-type strains are labelled with *." figureDoi="10.3897/mycokeys.102.113696.figure3" httpUri="https://binary.pensoft.net/fig/993279" pageId="0" pageNumber="201">3</figureCitation>
). Morphologically,
<taxonomicName id="8477AD5163BA8212AE9A3187CF59C135" class="Sordariomycetes" family="Sporocadaceae" genus="Neopestalotiopsis" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Neopestalotiopsis" order="Amphisphaeriales" pageId="0" pageNumber="201" phylum="Ascomycota" rank="genus">
<emphasis id="2C7FB2B4293CABCA5C624AB21F566DD6" italics="true" pageId="0" pageNumber="201">Neopestalotiopsis</emphasis>
</taxonomicName>
sp. 4 had shorter conidia and longer apical appendages (15.5-18.5
<normalizedToken id="04D17BCE33093F87FA8972BDC92F1B12" originalValue="×">x</normalizedToken>
6-8
<normalizedToken id="FDB0A7FD1ACF87C85D065363E8E3D83A" originalValue="µm">µm</normalizedToken>
, 9.5-15.5
<normalizedToken id="B0014C6EF026D1029EEC41B1EA5F18DD" originalValue="µm">µm</normalizedToken>
) than CFCC54340 and ZX22B (22-25.2
<normalizedToken id="35128EF841ADE864C9085BD38DD64E10" originalValue="×">x</normalizedToken>
6.2-7.7
<normalizedToken id="1925A96601A95952804C317CFA92D446" originalValue="µm">µm</normalizedToken>
, 5-10
<normalizedToken id="DD6F18C9A435F88B2EFC5666EFCB2DF5" originalValue="µm">µm</normalizedToken>
). In addition,
<taxonomicName id="50CEE75743E7170D7217275EE19F6B95" class="Sordariomycetes" family="Sporocadaceae" genus="Neopestalotiopsis" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Neopestalotiopsis" order="Amphisphaeriales" pageId="0" pageNumber="201" phylum="Ascomycota" rank="genus">
<emphasis id="F0FF7DF4CB1EA6FFDBA483682C998692" italics="true" pageId="0" pageNumber="201">Neopestalotiopsis</emphasis>
</taxonomicName>
sp. 4 had more tubular apical appendages (3-4, mostly 3) than
<taxonomicName id="7388F24E40C1060D0BB5E3A8A9CECE90" class="Sordariomycetes" family="Sporocadaceae" genus="Neopestalotiopsis" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Neopestalotiopsis" order="Amphisphaeriales" pageId="0" pageNumber="201" phylum="Ascomycota" rank="genus">
<emphasis id="6EA0B76A344AB9C1297B67859AD84A45" italics="true" pageId="0" pageNumber="201">Neopestalotiopsis</emphasis>
</taxonomicName>
sp. 2 (2, seldom 3). Compared with
<taxonomicName id="A5E1AD42A8A35362492AD4D14F3A620C" class="Sordariomycetes" family="Sporocadaceae" genus="Neopestalotiopsis" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Neopestalotiopsis" order="Amphisphaeriales" pageId="0" pageNumber="201" phylum="Ascomycota" rank="genus">
<emphasis id="B343BAE987E791975008F59F9A32CB26" italics="true" pageId="0" pageNumber="201">Neopestalotiopsis</emphasis>
</taxonomicName>
sp. 2 (ZX22B) and
<taxonomicName id="EBA2BE9E434C4E64101F0A2D13DA2B57" class="Sordariomycetes" family="Sporocadaceae" genus="Neopestalotiopsis" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Neopestalotiopsis" order="Amphisphaeriales" pageId="0" pageNumber="201" phylum="Ascomycota" rank="genus">
<emphasis id="3DA500D37FB30AE32ED7342E5D8AA0DA" italics="true" pageId="0" pageNumber="201">Neopestalotiopsis</emphasis>
</taxonomicName>
sp. 4, there were six nucleotide differences in the ITS region, five nucleotide differences in the
<emphasis id="4A08FA337C79129C9823372FBC898055" italics="true" pageId="0" pageNumber="201">tef-1α</emphasis>
region and three nucleotide differences in the
<emphasis id="C1C5C87FCD6DE11635C8A602FFF58CF1" italics="true" pageId="0" pageNumber="201">tub2</emphasis>
region. The taxonomy of the genera and species in this group remains unclear (
<bibRefCitation id="ACB8C8E654BC02A59DFFF54CF396D644" DOI="https://doi.org/10.3390/jof7121080" author="Li, L" journalOrPublisher="Journal of Fungi (Basel, Switzerland)" pageId="0" pageNumber="201" pagination=": 1080" refId="B25" refString="Li, L, Yang, Q, Li, H, 2021b. Morphology, phylogeny, and pathogenicity of Pestalotioid species on Camellia oleifera in China. Journal of Fungi (Basel, Switzerland) 7 (12): 1080, DOI: https://doi.org/10.3390/jof7121080" title="Morphology, phylogeny, and pathogenicity of Pestalotioid species on Camellia oleifera in China." url="https://doi.org/10.3390/jof7121080" volume="7" year="2021 b">Li et al. 2021b</bibRefCitation>
), as some clades were not unanimously strongly supported, meaning the genus will be largely revised in near future. Many taxa are likely to be synonymised in future publications. Therefore, CFCC59989 and CFCC59990 were not proposed as a new species.
</paragraph>
</subSubSection>
</treatment>
</document>