239 lines
18 KiB
XML
239 lines
18 KiB
XML
<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.82.66927" ID-GBIF-Dataset="18389681-011b-4f6f-b6a6-f49f0a5eb363" ID-PMC="PMC8367965" ID-Pensoft-Pub="1314-4049-82-81" ID-Pensoft-UUID="9E7F1A62FEE459ECBE50CD01D65EA103" ID-PubMed="34408539" ModsDocID="1314-4049-82-81" checkinTime="1628596891003" checkinUser="pensoft" docAuthor="Qu, Jiaojiao, Zou, Xiao, Cao, Wei, Xu, Zhongshun & Liang, Zongqi" docDate="2021" docId="F0BE3307271452AA83B6070FBE4087E0" docLanguage="en" docName="MycoKeys 82: 81-96" docOrigin="MycoKeys 82" docPubDate="2021-08-09" docSource="http://dx.doi.org/10.3897/mycokeys.82.66927" docTitle="Hirsutella kuankuoshuiensis X. Zou, J. J. Qu & Z. Q. Liang 2021, sp. nov." docType="treatment" docVersion="4" id="9E7F1A62FEE459ECBE50CD01D65EA103" lastPageNumber="81" masterDocId="9E7F1A62FEE459ECBE50CD01D65EA103" masterDocTitle="Two new species of Hirsutella (Ophiocordycipitaceae, Sordariomycetes) that are parasitic on lepidopteran insects from China" masterLastPageNumber="96" masterPageNumber="81" pageNumber="81" updateTime="1668136703672" updateUser="ExternalLinkService">
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<mods:mods xmlns:mods="http://www.loc.gov/mods/v3">
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<mods:titleInfo>
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<mods:title>Two new species of Hirsutella (Ophiocordycipitaceae, Sordariomycetes) that are parasitic on lepidopteran insects from China</mods:title>
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</mods:titleInfo>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Qu, Jiaojiao</mods:namePart>
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<mods:nameIdentifier type="ORCID">https://orcid.org/0000-0002-6401-4052</mods:nameIdentifier>
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<mods:affiliation>College of Tea Sciences, Guizhou University, Guiyang, 550025, China</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Zou, Xiao</mods:namePart>
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<mods:affiliation>Institute of Fungal Resources, College of Life Sciences, Guizhou University, Guiyang, 550025, China</mods:affiliation>
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<mods:nameIdentifier type="email">xzou@gzu.edu.cn</mods:nameIdentifier>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Cao, Wei</mods:namePart>
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<mods:affiliation>Institute of Fungal Resources, College of Life Sciences, Guizhou University, Guiyang, 550025, China</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Xu, Zhongshun</mods:namePart>
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<mods:affiliation>Institute of Fungal Resources, College of Life Sciences, Guizhou University, Guiyang, 550025, China</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Liang, Zongqi</mods:namePart>
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<mods:affiliation>Institute of Fungal Resources, College of Life Sciences, Guizhou University, Guiyang, 550025, China</mods:affiliation>
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</mods:name>
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<mods:typeOfResource>text</mods:typeOfResource>
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<mods:relatedItem type="host">
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<mods:titleInfo>
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<mods:title>MycoKeys</mods:title>
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</mods:titleInfo>
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<mods:part>
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<mods:date>2021</mods:date>
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<mods:detail type="pubDate">
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<mods:number>2021-08-09</mods:number>
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</mods:detail>
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<mods:detail type="volume">
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<mods:number>82</mods:number>
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</mods:detail>
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<mods:extent unit="page">
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<mods:start>81</mods:start>
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<mods:end>96</mods:end>
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</mods:extent>
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</mods:part>
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</mods:relatedItem>
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<mods:location>
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<mods:url>http://dx.doi.org/10.3897/mycokeys.82.66927</mods:url>
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</mods:location>
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<mods:classification>journal article</mods:classification>
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<mods:identifier type="DOI">http://dx.doi.org/10.3897/mycokeys.82.66927</mods:identifier>
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<mods:identifier type="Pensoft-Pub">1314-4049-82-81</mods:identifier>
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<mods:identifier type="Pensoft-UUID">9E7F1A62FEE459ECBE50CD01D65EA103</mods:identifier>
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</mods:mods>
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<subSection pageId="0" pageNumber="81" type="results">
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<treatment ID-GBIF-Taxon="184008572" LSID="urn:lsid:plazi:treatment:F0BE3307271452AA83B6070FBE4087E0" httpUri="http://treatment.plazi.org/id/F0BE3307271452AA83B6070FBE4087E0" lastPageNumber="81" pageId="0" pageNumber="81">
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<subSubSection pageId="0" pageNumber="81" type="nomenclature">
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<paragraph pageId="0" pageNumber="81">
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<taxonomicName LSID="F0BE3307-2714-52AA-83B6-070FBE4087E0" authority="X. Zou, J. J. Qu & Z. Q. Liang" authorityName="X. Zou, J. J. Qu & Z. Q. Liang" authorityYear="2021" class="Sordariomycetes" family="Ophiocordycipitaceae" genus="Hirsutella" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Hirsutella kuankuoshuiensis" order="Hypocreales" pageId="0" pageNumber="81" phylum="Ascomycota" rank="species" species="kuankuoshuiensis" status="sp. nov.">Hirsutella kuankuoshuiensis X. Zou, J.J. Qu & Z.Q. Liang</taxonomicName>
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<taxonomicNameLabel pageId="0" pageNumber="81">sp. nov.</taxonomicNameLabel>
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="81" type="description">
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<paragraph pageId="0" pageNumber="81">
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<figureCitation captionStart="Figure 3" captionStartId="F3" captionText="Figure 3. Morphological characteristics of Hirsutella kuankuoshuiensis A the insect specimens with single and thin synnemata (HKAS 112885) B, C colonial morphology on PDA agar media for 20 d B shows the front of the colony and C shows the back of the colony D-G LM images showing conidiogenous cells and conidia D, E the structure of conidiogenous cells on mycelia F the images of conidiogenous cells on synnemata (optical microscope) H-J conidial morphology (LM) G conidia with mucilage (SEM). Scale bars: 10 mm (A-C); bar of G was shown in the figure; the rest of the bars were 10 μm. LM, light microscopy; PDA, potato dextrose agar; SEM, scanning electron microscopy." figureDoi="10.3897/mycokeys.82.66927.figure3" httpUri="https://binary.pensoft.net/fig/575890" pageId="0" pageNumber="81">Fig. 3</figureCitation>
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="81" type="diagnosis">
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<paragraph pageId="0" pageNumber="81">Diagnosis.</paragraph>
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<paragraph pageId="0" pageNumber="81">
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<taxonomicName authorityName="X. Zou, J. J. Qu & Z. Q. Liang" authorityYear="2021" class="Sordariomycetes" family="Ophiocordycipitaceae" genus="Hirsutella" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Hirsutella kuankuoshuiensis" order="Lepidoptera" pageId="0" pageNumber="81" phylum="Ascomycota" rank="species" species="kuankuoshuiensis">
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<emphasis italics="true" pageId="0" pageNumber="81">Hirsutella kuankuoshuiensis</emphasis>
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</taxonomicName>
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differs from other species in this genus primarily by its clavate, narrow fusiform or botuliform conidia and subulate or slender columnar phialide.
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="81" type="materials_examined">
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<paragraph pageId="0" pageNumber="81">
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<typeStatus>Type</typeStatus>
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.
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</paragraph>
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<paragraph pageId="0" pageNumber="81">
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<materialsCitation collectingDate="2012-07" collectorName="X. Zou. The, Sequences, GenBank" country="China" county="Suiyang County" elevation="1400" latitude="28.133333" location="Kuankuoshui Nature Reserve" longLatPrecision="1233" longitude="107.03333" specimenCount="1" stateProvince="Guizhou Province" typeStatus="holotype">
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<collectingCountry name="China">China</collectingCountry>
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,
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<collectingRegion country="China" name="Guizhou">Guizhou Province</collectingRegion>
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,
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<collectingCounty>Suiyang County</collectingCounty>
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,
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<location LSID="urn:lsid:plazi:treatment:F0BE3307271452AA83B6070FBE4087E0:280672CFB10F694312E19DFBDFE96761" country="China" county="Suiyang County" latitude="28.133333" longLatPrecision="1233" longitude="107.03333" name="Kuankuoshui Nature Reserve" stateProvince="Guizhou Province">Kuankuoshui Nature Reserve</location>
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(
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<geoCoordinate degrees="28" direction="north" minutes="08" orientation="latitude" precision="925" value="28.133333">28°08'N</geoCoordinate>
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,
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<geoCoordinate degrees="107" direction="east" minutes="02" orientation="longitude" precision="925" value="107.03333">107°02'E</geoCoordinate>
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, approximately
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<elevation metricMagnitude="3" metricUnit="m" metricValue="1.4" unit="m" value="1400.0">
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<quantity metricMagnitude="3" metricUnit="m" metricValue="1.4" unit="m" value="1400.0">1400 m</quantity>
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a.s.l.
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</elevation>
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),
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<collectingDate value="2012-07">July 2012</collectingDate>
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, collected by
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<collectorName>X. Zou. The</collectorName>
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<typeStatus>holotype</typeStatus>
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has been deposited at KIB (HKAS112885).
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<collectorName>Sequences</collectorName>
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from isolated strains (GZUIFR-2012KKS3-1, GZUIFR-2012KKS3-2 and GZUIFR-2012KKS3-3) have been deposited in
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<collectorName>GenBank</collectorName>
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</materialsCitation>
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.
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="81" type="description">
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<paragraph pageId="0" pageNumber="81">Description.</paragraph>
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<paragraph pageId="0" pageNumber="81">
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<taxonomicName genus="Synnemata" lsidName="Synnemata" pageId="0" pageNumber="81" rank="genus">
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<emphasis italics="true" pageId="0" pageNumber="81">Synnemata</emphasis>
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</taxonomicName>
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are single, extending from the head of insect; 8.6 cm long, dark brown and changing to brown towards the apex; no conidiation was observed (Fig.
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<figureCitation captionStart="Figure 3" captionStartId="F3" captionText="Figure 3. Morphological characteristics of Hirsutella kuankuoshuiensis A the insect specimens with single and thin synnemata (HKAS 112885) B, C colonial morphology on PDA agar media for 20 d B shows the front of the colony and C shows the back of the colony D-G LM images showing conidiogenous cells and conidia D, E the structure of conidiogenous cells on mycelia F the images of conidiogenous cells on synnemata (optical microscope) H-J conidial morphology (LM) G conidia with mucilage (SEM). Scale bars: 10 mm (A-C); bar of G was shown in the figure; the rest of the bars were 10 μm. LM, light microscopy; PDA, potato dextrose agar; SEM, scanning electron microscopy." figureDoi="10.3897/mycokeys.82.66927.figure3" httpUri="https://binary.pensoft.net/fig/575890" pageId="0" pageNumber="81">3A</figureCitation>
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). The fungus spreads slowly on PDA agar at 20-22 °C and grows to a diam. of 22-30 mm after 14 d; the colony is round, centre of surface with brown dense bulges and grey-white sparse flocculent aerial hyphae. Colony margin is flat with radial groove; a large amount of brown pigment secreted into the medium causes the back of colony to appear dark brown; thickness 10-12 mm (Fig.
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<figureCitation captionStart="Figure 3" captionStartId="F3" captionText="Figure 3. Morphological characteristics of Hirsutella kuankuoshuiensis A the insect specimens with single and thin synnemata (HKAS 112885) B, C colonial morphology on PDA agar media for 20 d B shows the front of the colony and C shows the back of the colony D-G LM images showing conidiogenous cells and conidia D, E the structure of conidiogenous cells on mycelia F the images of conidiogenous cells on synnemata (optical microscope) H-J conidial morphology (LM) G conidia with mucilage (SEM). Scale bars: 10 mm (A-C); bar of G was shown in the figure; the rest of the bars were 10 μm. LM, light microscopy; PDA, potato dextrose agar; SEM, scanning electron microscopy." figureDoi="10.3897/mycokeys.82.66927.figure3" httpUri="https://binary.pensoft.net/fig/575890" pageId="0" pageNumber="81">3B, C</figureCitation>
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).
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<emphasis italics="true" pageId="0" pageNumber="81">Mycelium</emphasis>
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hyaline, smooth, septate, 1.5-3.0
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<normalizedToken originalValue="μm">μm</normalizedToken>
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wide.
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<emphasis italics="true" pageId="0" pageNumber="81">Conidiogenous cells</emphasis>
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monophialidic, hyaline, borne perpendicular or at an acute angle to the subtending hyphae.
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<emphasis italics="true" pageId="0" pageNumber="81">Phialides</emphasis>
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subulate or slender columnar, tapering gradually to a long and narrow neck, 30-45
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<normalizedToken originalValue="×">x</normalizedToken>
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1-3
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<normalizedToken originalValue="μm">μm</normalizedToken>
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long.
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<emphasis italics="true" pageId="0" pageNumber="81">Conidia</emphasis>
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clavate, narrow fusiform or botuliform without a diaphragm, 9.9-12.6
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<normalizedToken originalValue="×">x</normalizedToken>
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2.7-4.5
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<normalizedToken originalValue="μm">μm</normalizedToken>
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, single- or double-enveloped in a hyaline mucus, thickness 2.0-3.0
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<normalizedToken originalValue="μm">μm</normalizedToken>
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(Fig.
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<figureCitation captionStart="Figure 3" captionStartId="F3" captionText="Figure 3. Morphological characteristics of Hirsutella kuankuoshuiensis A the insect specimens with single and thin synnemata (HKAS 112885) B, C colonial morphology on PDA agar media for 20 d B shows the front of the colony and C shows the back of the colony D-G LM images showing conidiogenous cells and conidia D, E the structure of conidiogenous cells on mycelia F the images of conidiogenous cells on synnemata (optical microscope) H-J conidial morphology (LM) G conidia with mucilage (SEM). Scale bars: 10 mm (A-C); bar of G was shown in the figure; the rest of the bars were 10 μm. LM, light microscopy; PDA, potato dextrose agar; SEM, scanning electron microscopy." figureDoi="10.3897/mycokeys.82.66927.figure3" httpUri="https://binary.pensoft.net/fig/575890" pageId="0" pageNumber="81">3D-J</figureCitation>
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).
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</paragraph>
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<caption doi="10.3897/mycokeys.82.66927.figure3" httpUri="https://binary.pensoft.net/fig/575890" pageId="0" pageNumber="81" start="Figure 3" startId="F3">
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<paragraph pageId="0" pageNumber="81">
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<emphasis bold="true" pageId="0" pageNumber="81">Figure 3.</emphasis>
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Morphological characteristics of
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<taxonomicName authorityName="X. Zou, J. J. Qu & Z. Q. Liang" authorityYear="2021" class="Sordariomycetes" family="Ophiocordycipitaceae" genus="Hirsutella" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Hirsutella kuankuoshuiensis" order="Lepidoptera" pageId="0" pageNumber="81" phylum="Ascomycota" rank="species" species="kuankuoshuiensis">
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<emphasis italics="true" pageId="0" pageNumber="81">Hirsutella kuankuoshuiensis</emphasis>
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</taxonomicName>
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<emphasis bold="true" pageId="0" pageNumber="81">A</emphasis>
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the insect specimens with single and thin synnemata (HKAS112885)
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<emphasis bold="true" pageId="0" pageNumber="81">B, C</emphasis>
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colonial morphology on PDA agar media for 20 d
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<emphasis bold="true" pageId="0" pageNumber="81">B</emphasis>
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shows the front of the colony and
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<emphasis bold="true" pageId="0" pageNumber="81">C</emphasis>
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shows the back of the colony
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<emphasis bold="true" pageId="0" pageNumber="81">D-G</emphasis>
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LM images showing conidiogenous cells and conidia
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<emphasis bold="true" pageId="0" pageNumber="81">D, E</emphasis>
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the structure of conidiogenous cells on mycelia
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<emphasis bold="true" pageId="0" pageNumber="81">F</emphasis>
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the images of conidiogenous cells on synnemata (optical microscope)
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<emphasis bold="true" pageId="0" pageNumber="81">H-J</emphasis>
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conidial morphology (LM)
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<emphasis bold="true" pageId="0" pageNumber="81">G</emphasis>
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conidia with mucilage (SEM). Scale bars: 10 mm (
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<emphasis bold="true" pageId="0" pageNumber="81">A-C</emphasis>
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); bar of
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<emphasis bold="true" pageId="0" pageNumber="81">G</emphasis>
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was shown in the figure; the rest of the bars were 10
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<normalizedToken originalValue="μm">μm</normalizedToken>
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. LM, light microscopy; PDA, potato dextrose agar; SEM, scanning electron microscopy.
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</paragraph>
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</caption>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="81" type="etymology">
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<paragraph pageId="0" pageNumber="81">Etymology.</paragraph>
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<paragraph pageId="0" pageNumber="81">
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Referring to the locality of the specimen, kuankuoshui (Lat. '
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<taxonomicName authorityName="X. Zou, J. J. Qu & Z. Q. Liang" authorityYear="2021" class="Sordariomycetes" family="Ophiocordycipitaceae" genus="Hirsutella" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Hirsutella kuankuoshuiensis" order="Lepidoptera" pageId="0" pageNumber="81" phylum="Ascomycota" rank="species" species="kuankuoshuiensis">Hirsutella kuankuoshuiensis</taxonomicName>
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').
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="81" type="host">
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<paragraph pageId="0" pageNumber="81">Host.</paragraph>
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<paragraph pageId="0" pageNumber="81">
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<taxonomicName kingdom="Fungi" lsidName="" order="Lepidoptera" pageId="0" pageNumber="81" rank="order">Lepidoptera</taxonomicName>
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larva.
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="81" type="habitat">
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<paragraph pageId="0" pageNumber="81">Habitat and distribution.</paragraph>
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<paragraph pageId="0" pageNumber="81">On the decaying leaves of broadleaved forests, Guizhou Province, China.</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="81" type="teleomorph">
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<paragraph pageId="0" pageNumber="81">Teleomorph.</paragraph>
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<paragraph pageId="0" pageNumber="81">Unknown.</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="81" type="remarks">
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<paragraph pageId="0" pageNumber="81">Remarks.</paragraph>
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<paragraph pageId="0" pageNumber="81">
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This species possesses two types of conidiogenous cells and long fusiform or clavate without diaphragm conidia (9.9-12.6
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<normalizedToken originalValue="×">x</normalizedToken>
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2.7-4.5
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<normalizedToken originalValue="μm">μm</normalizedToken>
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), which is extremely rare in
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<taxonomicName class="Sordariomycetes" family="Ophiocordycipitaceae" genus="Hirsutella" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Hirsutella" order="Lepidoptera" pageId="0" pageNumber="81" phylum="Ascomycota" rank="genus">
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<emphasis italics="true" pageId="0" pageNumber="81">Hirsutella</emphasis>
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</taxonomicName>
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species. In addition,
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<taxonomicName class="Insecta" family="Microlepidoptea" kingdom="Animalia" lsidName="H. kuankuoshuiensis" order="Lepidoptera" pageId="0" pageNumber="81" phylum="Arthropoda" rank="species" species="kuankuoshuiensis">
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<emphasis italics="true" pageId="0" pageNumber="81">H. kuankuoshuiensis</emphasis>
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</taxonomicName>
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could produce long thin synnemata on the culture media that contain few or no conidia.
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</paragraph>
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</subSubSection>
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</treatment>
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</subSection>
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</document> |