treatments-xml/data/D0/D7/00/D0D7002219445506A79E318281D5606A.xml
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<document ID-CLB-Dataset="284557" ID-DOI="http://dx.doi.org/10.3897/mycokeys.101.113128" ID-GBIF-Dataset="95f21583-b8dd-4bc3-8b00-aa1049f4c962" ID-Pensoft-Pub="1314-4049-101-45" ID-Pensoft-UUID="3CCDD1D514AB5025BB9D7067379B93B2" ModsDocID="1314-4049-101-45" checkinTime="1704767822250" checkinUser="pensoft" docAuthor="He, Jiao, Li, De-Wei, Cui, Wen-Li, Zhu, Li-Hua &amp; Huang, Lin" docDate="2024" docId="D0D7002219445506A79E318281D5606A" docLanguage="en" docName="MycoKeys 101: 45-80" docOrigin="MycoKeys 101" docPubDate="2024-01-08" docSource="http://dx.doi.org/10.3897/mycokeys.101.113128" docTitle="Fusarium hunanense Lin Huang, Jiao He &amp; D. W. Li 2024, sp. nov." docType="treatment" docVersion="2" id="3CCDD1D514AB5025BB9D7067379B93B2" lastPageNumber="45" masterDocId="3CCDD1D514AB5025BB9D7067379B93B2" masterDocTitle="Morphological and phylogenetic analyses reveal three new species of Fusarium (Hypocreales, Nectriaceae) associated with leaf blight on Cunninghamia lanceolata in China" masterLastPageNumber="80" masterPageNumber="45" pageNumber="45" updateTime="1704768447767" updateUser="ExternalLinkService">
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<mods:title id="653376C9610AF4457DFE4268C683F915">Morphological and phylogenetic analyses reveal three new species of Fusarium (Hypocreales, Nectriaceae) associated with leaf blight on Cunninghamia lanceolata in China</mods:title>
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<mods:namePart id="F9D8DB329140A217D6A03B9A23B9E0DB">He, Jiao</mods:namePart>
<mods:nameIdentifier id="73AC2A8E4FD9C62A5155DBE9032E2039" type="ORCID">https://orcid.org/0000-0002-4146-2223</mods:nameIdentifier>
<mods:affiliation id="DAFE698FC23F69DB706B72A3B7F7C699">Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, Jiangsu 210037, China</mods:affiliation>
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<mods:namePart id="1D8AD4FF9AF5C0758F766849F5C4AAD4">Li, De-Wei</mods:namePart>
<mods:nameIdentifier id="10B4E2DFC1AC57141FC58762DE5D377A" type="ORCID">https://orcid.org/0000-0002-2788-7938</mods:nameIdentifier>
<mods:affiliation id="7C694270982A03354F9F321E091D442A">The Connecticut Agricultural Experiment Station Valley Laboratory, Windsor, CT 06095, USA</mods:affiliation>
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<mods:namePart id="D2C4ABBABEB1A2C06FAA0CF3A4FEF40C">Cui, Wen-Li</mods:namePart>
<mods:nameIdentifier id="86D5B7AFCF49172DFAEB7792C452A9CA" type="ORCID">https://orcid.org/0009-0005-7515-7672</mods:nameIdentifier>
<mods:affiliation id="E844720D8A29ED2D2C30E0AE3204517D">Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, Jiangsu 210037, China</mods:affiliation>
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<mods:namePart id="C6C3AD4DC1D7E023A9E2A45436EB6868">Zhu, Li-Hua</mods:namePart>
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<mods:affiliation id="88A2C487BDCC8C9842BE2C0E3AC0E5CD">Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, Jiangsu 210037, China</mods:affiliation>
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<mods:namePart id="8AE6AA95FC6704DA0CBF5D29BFDEE021">Huang, Lin</mods:namePart>
<mods:nameIdentifier id="2D9188126B8D2BF6220167117ECB2008" type="ORCID">https://orcid.org/0000-0001-7536-0914</mods:nameIdentifier>
<mods:affiliation id="49B0F6B9616D15A787CBD5F76EEC68B3">Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, Jiangsu 210037, China</mods:affiliation>
<mods:nameIdentifier id="C4B05C98247154390686427233BF1466" type="email">lhuang@njfu.edu.cn</mods:nameIdentifier>
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<mods:date id="57A2AD42099A90C1AB2E4741FD84AA3F">2024</mods:date>
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<mods:number id="6541BD1F58AFEDBCFFB94DEEBA89EA9E">2024-01-08</mods:number>
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<mods:identifier id="524026755EC8C254F08A8CB21B8A8B78" type="DOI">http://dx.doi.org/10.3897/mycokeys.101.113128</mods:identifier>
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<subSection id="868BCC05FE86390DD7EFCFFF2D9F6D52" pageId="0" pageNumber="45" type="results">
<treatment id="D0D7002219445506A79E318281D5606A" LSID="urn:lsid:plazi:treatment:D0D7002219445506A79E318281D5606A" httpUri="http://treatment.plazi.org/id/D0D7002219445506A79E318281D5606A" lastPageNumber="45" pageId="0" pageNumber="45">
<subSubSection id="800B2EF9AE75ABE6D3B1A91F59C62FFA" pageId="0" pageNumber="45" type="nomenclature">
<paragraph id="A53BDC14E486D42B589402AFA9129867" pageId="0" pageNumber="45">
<taxonomicName id="E7E41B81A00324954E7036F47DA417B7" LSID="D0D70022-1944-5506-A79E-318281D5606A" authority="Lin Huang, Jiao He &amp; D. W. Li" authorityName="Lin Huang, Jiao He &amp; D. W. Li" authorityYear="2024" class="Ascomycetes" family="Nectriaceae" genus="Fusarium" higherTaxonomySource="treatment-meta" kingdom="Fungi" lsidName="Fusarium hunanense" order="Hypocreales" pageId="0" pageNumber="45" phylum="Ascomycota" rank="species" species="hunanense" status="sp. nov.">Fusarium hunanense Lin Huang, Jiao He &amp; D.W. Li</taxonomicName>
<taxonomicNameLabel id="373B789727492C67CFB2AB8B0DAAA3F8" pageId="0" pageNumber="45">sp. nov.</taxonomicNameLabel>
</paragraph>
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<paragraph id="7FED0F4D275EE0AA1F88D7645A8DB629" pageId="0" pageNumber="45">
<figureCitation id="4DF3C4B5754BBFEBA6D1A35AE4D385E6" captionStart="Figure 8" captionStartId="F8" captionText="Figure 8. Fusarium hunanense (HN 33 - 8 - 2) A-D colonies on PDA, SNA, OMA, and CMA, respectively, after 5 days at 24 ° C in the dark E sporodochia formed on PDA F-K aerial conidiophores, phialides, and conidia L-N sporodochial conidiophores, phialides, and conidia O, P macroconidia (3 - 6 - septate) Q chlamydospore. Scale bars: 1,000 μm (E); 50 μm (F-H); 10 μm (I-Q)." figureDoi="10.3897/mycokeys.101.113128.figure8" httpUri="https://binary.pensoft.net/fig/963605" pageId="0" pageNumber="45">Fig. 8</figureCitation>
</paragraph>
</subSubSection>
<subSubSection id="3AFCB0877C50F640243A5D1D5933EE1E" pageId="0" pageNumber="45" type="etymology">
<paragraph id="5DA1FDFE8F61FA0EB766339C7392B51D" pageId="0" pageNumber="45">Etymology.</paragraph>
<paragraph id="A511B9AD45D483DEA1F27B023D74946D" pageId="0" pageNumber="45">Epithet is named after Hunan Province where the type specimen was collected.</paragraph>
</subSubSection>
<subSubSection id="534690F0565BEA729B725F11E9E54009" pageId="0" pageNumber="45" type="holotype">
<paragraph id="64C9CC6E02AC75A00E488FCB30276A45" pageId="0" pageNumber="45">Holotype.</paragraph>
<paragraph id="886D0F241C0E575E6520B768453D14B3" pageId="0" pageNumber="45">
China, Hunan Province, Yiyang City, Heshan District, Henglongqiao Town,
<geoCoordinate id="02B5761E347D9489F324B95A579E7DCC" degrees="28" direction="north" minutes="27" orientation="latitude" precision="15" seconds="24" value="28.456667">28°2724″N</geoCoordinate>
,
<geoCoordinate id="9553D167451D67A18326D30CC550E74F" degrees="112" direction="east" minutes="29" orientation="longitude" precision="15" seconds="7" value="112.485275">112°297″E</geoCoordinate>
, isolated from leaf spots of
<taxonomicName id="401D26A2F71BF206A8597FDA28ACD9A1" class="Sordariomycetes" family="Nectriaceae" genus="Cunninghamia" kingdom="Fungi" lsidName="Cunninghamia lanceolata" order="Hypocreales" pageId="0" pageNumber="45" phylum="Ascomycota" rank="species" species="lanceolata">
<emphasis id="5929DB72C1D8C8F6A40D34DD4145C438" italics="true" pageId="0" pageNumber="45">Cunninghamia lanceolata</emphasis>
</taxonomicName>
, May 2017, Wen-Li Cui, (holotype: CFCC 57574). Holotype specimen is a living specimen maintained via lyophilization at the China Forestry Culture Collection Center (CFCC). Ex-type (HN33-8-2) is maintained at the Forest Pathology Laboratory, Nanjing Forestry University.
</paragraph>
<caption id="AA69717AA0781643569ACC2D6842D022" doi="10.3897/mycokeys.101.113128.figure8" httpUri="https://binary.pensoft.net/fig/963605" pageId="0" pageNumber="45" start="Figure 8" startId="F8">
<paragraph id="228169A3371B9316F339B03E7669F72E" pageId="0" pageNumber="45">
<emphasis id="D2768A564BBF559749E26B3B5A20349F" bold="true" pageId="0" pageNumber="45">Figure 8.</emphasis>
<taxonomicName id="D51C97D78EC51AD6956C3E46ADE66A57" authorityName="Lin Huang, Jiao He &amp; D. W. Li" authorityYear="2024" class="Ascomycetes" family="Nectriaceae" genus="Fusarium" kingdom="Fungi" lsidName="Fusarium hunanense" order="Hypocreales" pageId="0" pageNumber="45" phylum="Ascomycota" rank="species" species="hunanense">
<emphasis id="680D32CC0A643BC5DF7FDF722C2A8992" italics="true" pageId="0" pageNumber="45">Fusarium hunanense</emphasis>
</taxonomicName>
(HN33-8-2)
<emphasis id="2CACC3B431D7DFE52DE408DE65671113" bold="true" pageId="0" pageNumber="45">A-D</emphasis>
colonies on PDA, SNA, OMA, and CMA, respectively, after 5 days at 24 °C in the dark
<emphasis id="903C3AA4CA0FBE8FADA08A91D7EA4259" bold="true" pageId="0" pageNumber="45">E</emphasis>
sporodochia formed on PDA
<emphasis id="02F96382B1832B7E62BA901CE19A00A6" bold="true" pageId="0" pageNumber="45">F-K</emphasis>
aerial conidiophores, phialides, and conidia
<emphasis id="9F5F28D2415704FEAF967E7DE0613ABB" bold="true" pageId="0" pageNumber="45">L-N</emphasis>
sporodochial conidiophores, phialides, and conidia
<emphasis id="EB34E62EA2CB875647D3C3E1541B61FD" bold="true" pageId="0" pageNumber="45">O, P</emphasis>
macroconidia (3-6-septate)
<emphasis id="6C103545F31D97D4AAF0E14003A42A9C" bold="true" pageId="0" pageNumber="45">Q</emphasis>
chlamydospore. Scale bars: 1,000
<normalizedToken id="D2B932725154C7A56162904D8358E5EB" originalValue="μm">μm</normalizedToken>
(
<emphasis id="04B25D3C52BA0898D0EC4C1B2267B734" bold="true" pageId="0" pageNumber="45">E</emphasis>
); 50
<normalizedToken id="72F2FE9DFDC904D207A42F3C6552B63F" originalValue="μm">μm</normalizedToken>
(
<emphasis id="C600652A7D4118595DBC58791B2C3BAD" bold="true" pageId="0" pageNumber="45">F-H</emphasis>
); 10
<normalizedToken id="71E51F91F0E916EAF6653934DC007530" originalValue="μm">μm</normalizedToken>
(
<emphasis id="B4948368E830EC85AAB068BEC90B1260" bold="true" pageId="0" pageNumber="45">I-Q</emphasis>
).
</paragraph>
</caption>
</subSubSection>
<subSubSection id="623D44E98E4C675DF73E15F353BFD5D4" pageId="0" pageNumber="45" type="distribution">
<paragraph id="20D350E27E1687121CBDE09998BF5091" pageId="0" pageNumber="45">Host/distribution.</paragraph>
<paragraph id="02361E67F9E36DEB33033F2506E3FBE4" pageId="0" pageNumber="45">
From
<taxonomicName id="9F5E81171C8ABDF39ED46FC313CF8B8C" lsidName="C. lanceolata" pageId="0" pageNumber="45" rank="species" species="lanceolata">
<emphasis id="CF764AD3785C4EF8AE058CBB166F5586" italics="true" pageId="0" pageNumber="45">C. lanceolata</emphasis>
</taxonomicName>
in Henglongqiao Town, Heshan District, Yiyang City, Hunan Province, China.
</paragraph>
</subSubSection>
<subSubSection id="6C83BE4038146E01F9E675D87228B740" pageId="0" pageNumber="45" type="description">
<paragraph id="BD8311012EA3E25971051A3B6EC442B2" pageId="0" pageNumber="45">Description.</paragraph>
<paragraph id="EE0F91769A068D42BC1DFAFB1E0E8419" pageId="0" pageNumber="45">
Sexual state not observed. Asexual state: sporulation abundant from erect conidiophores formed on the agar surface or aggregated in sporodochia. Conidiophores in the aerial mycelium, mostly unbranched, rarely basally dichotomously branched, forming monophialides on the apices; phialides slender, subulate to subcylindrical, monophialidic, smooth, thin-walled, (29.6-)31.6-54.6(-74.1)
<normalizedToken id="F867756EDAD212A83FE07ACD92823927" originalValue="×">x</normalizedToken>
(2.0-)2.2-2.8(-3.0)
<normalizedToken id="DABC93E6313CC39901C55B20B320F626" originalValue="μm">μm</normalizedToken>
, (mean
<normalizedToken id="5AD40215C7EDC9F02746814C0F78D756" originalValue="±">+/-</normalizedToken>
SD = 43.1
<normalizedToken id="05579FB7E7C3B0CC275855D6FBE59F90" originalValue="±">+/-</normalizedToken>
11.5
<normalizedToken id="6AEB601956B555C3D6D1FBE8E393CE5E" originalValue="×">x</normalizedToken>
2.5
<normalizedToken id="65837C53FB4F6DE7F84723AA16F4AE42" originalValue="±">+/-</normalizedToken>
0.3
<normalizedToken id="0FB017BAC6798FB8CDCC44251A8D8052" originalValue="μm">μm</normalizedToken>
, n = 17), with slight periclinal thickening at the tip and a short flared apical collarette. Sporodochia cream colored, produced on the surface of carnation leaves and PDA medium. Conidiophores in sporodochia (26.0-)29.3-39.1(-46.8)
<normalizedToken id="9243128956AFEC9992E76074C83BB06B" originalValue="μm">μm</normalizedToken>
, (mean
<normalizedToken id="72AF703357DBCFC46F7D7939E97B5C8C" originalValue="±">+/-</normalizedToken>
SD = 34.1
<normalizedToken id="1AFF47ECFB8C0BDE267030938D4E811C" originalValue="±">+/-</normalizedToken>
5.1
<normalizedToken id="EA5AAF460533BB957D32F40140C3CB2C" originalValue="μm">μm</normalizedToken>
, n = 39), irregularly branched, short stipitate, occasionally in whorls bearing terminal 2-4 monophialides; sporodochial phialides subulate to subcylindrical, smooth, thin-walled, (11.4-)15.5-22.1(-28.6)
<normalizedToken id="3B516FF7CE931CF31B9E35097D4368FB" originalValue="×">x</normalizedToken>
(3.3-)4.0-5.2(-6.0)
<normalizedToken id="738AA32A58B3DA586DB5D67D14213045" originalValue="μm">μm</normalizedToken>
, (mean
<normalizedToken id="5E757DF6F5D7C734E336F9AA1B15192E" originalValue="±">+/-</normalizedToken>
SD = 18.8
<normalizedToken id="CB20B3C25959F1FCC31BC3EDE1A25BC4" originalValue="±">+/-</normalizedToken>
3.3
<normalizedToken id="A35FD4D1E281EFFBAB6B8A59C386757A" originalValue="×">x</normalizedToken>
4.6
<normalizedToken id="78CFF82CC79572E6C0010E7B40B5FB09" originalValue="±">+/-</normalizedToken>
0.6
<normalizedToken id="D278D1A25EC38B6C7D281C629F8E6298" originalValue="μm">μm</normalizedToken>
, n = 51), with periclinal thickening and a small, flared collarette. Sporodochial macroconidia cylindrical to falcate, gently curved, typically with a blunt and almost rounded apical cell and a barely notched foot cell, 3-6-septate, hyaline, smooth, thin-walled. Three-septate conidia: (22.1-)22.6-39.4(-54.7)
<normalizedToken id="5C090DA522BE77151D2AE77B8232E12E" originalValue="×">x</normalizedToken>
(5.0-)5.5-6.7(-7.4)
<normalizedToken id="75FA6E2339077B358242F8E344430DB4" originalValue="μm">μm</normalizedToken>
, (mean
<normalizedToken id="8C0CACBD304E19C1A07E5F40AA683B0F" originalValue="±">+/-</normalizedToken>
SD = 31.0
<normalizedToken id="05F297EB656CE046758915496950622E" originalValue="±">+/-</normalizedToken>
8.4
<normalizedToken id="9C1A39E8FDD32C66B66E41824E3303F9" originalValue="×">x</normalizedToken>
6.1
<normalizedToken id="58E71989AE7F84787DBF06E15EE6A287" originalValue="±">+/-</normalizedToken>
0.6
<normalizedToken id="94CDCE5496B88E4C6EC01B591C145013" originalValue="μm">μm</normalizedToken>
, n = 11); four-septate conidia: (50.3-)54.4-68.2(-69.6)
<normalizedToken id="CA2332684E414129F4753E812C375980" originalValue="×">x</normalizedToken>
(6.9-)6.9-7.7(-8.0)
<normalizedToken id="33977624E6BFAB7A7DBBF7D21A4C417B" originalValue="μm">μm</normalizedToken>
, (mean
<normalizedToken id="C6A13A8F086687D1D9C8B93E7F751875" originalValue="±">+/-</normalizedToken>
SD = 61.3
<normalizedToken id="415EA0F3824BE82622B8A00CE2F9BD18" originalValue="±">+/-</normalizedToken>
6.9
<normalizedToken id="B1A3EF2F9EDE7186F605933ED852B7E4" originalValue="×">x</normalizedToken>
7.3
<normalizedToken id="114501AD4D6BFD9015146BF5673E341A" originalValue="±">+/-</normalizedToken>
0.4
<normalizedToken id="51DF4508B9A7FA74EA2F299496050F2B" originalValue="μm">μm</normalizedToken>
, n = 10); five-septate conidia: (51.8-)60.6-73.0(-78.2)
<normalizedToken id="788D5AC33AAA8D57D890F09202661738" originalValue="×">x</normalizedToken>
(6.4-)6.1-7.1(-8.5)
<normalizedToken id="3864DDA69AD84E4BB06CB83DD1D7587E" originalValue="μm">μm</normalizedToken>
, (mean
<normalizedToken id="D0AAF3A89A9C70401A0718E3E615C898" originalValue="±">+/-</normalizedToken>
SD = 66.8
<normalizedToken id="D2107C6937F64AA04D51A1222F2CB609" originalValue="±">+/-</normalizedToken>
6.2
<normalizedToken id="D46CB4ADB53A8574F59376512871216F" originalValue="×">x</normalizedToken>
6.6
<normalizedToken id="14B71866918F9C45B31BC93E0A178D4D" originalValue="±">+/-</normalizedToken>
0.5
<normalizedToken id="69552628A5FC07294711D5BEFD5CE05A" originalValue="μm">μm</normalizedToken>
, n = 31); six-septate conidia: (69.8-)70.7-77.7(-79.6)
<normalizedToken id="F8E3682062181C8E17BFB8C9AD71ADAF" originalValue="×">x</normalizedToken>
(7.1-)7.5-8.3(-8.3)
<normalizedToken id="A612CFF4B3B4A9CC66096618E3A69560" originalValue="μm">μm</normalizedToken>
, (mean
<normalizedToken id="3147040E7D0753913EE76B81796F7736" originalValue="±">+/-</normalizedToken>
SD = 74.2
<normalizedToken id="A717B6469D340A97EE00FD8ECF4DE0B3" originalValue="±">+/-</normalizedToken>
3.5
<normalizedToken id="D0C57CF5773E445778D6FE0423C07687" originalValue="μm">μm</normalizedToken>
<normalizedToken id="025EF0CF93B3329674204A5599B91920" originalValue="×">x</normalizedToken>
7.9
<normalizedToken id="D023031F71B0E8005725A53C7A352B0A" originalValue="±">+/-</normalizedToken>
0.4
<normalizedToken id="EB4D993DDBF933304B56FBFA4104AF30" originalValue="μm">μm</normalizedToken>
, n = 10). Chlamydospores developed in large numbers in hyphae and also in mature macroconidia. The chlamydospores were 0-1-septate, globose to ellipsoidal, constricted at the septum, intercalary or terminal in chains or solitary with mostly a pale color and smooth, (11.7-)11.7-12.9(-13.5)
<normalizedToken id="A4647B5C1F50FCE0586C9CE350DCEC4C" originalValue="×">x</normalizedToken>
(7.7-)7.7-8.5(-8.6)
<normalizedToken id="57BA8E044F4B2114B7C63253BF3BE205" originalValue="μm">μm</normalizedToken>
, (mean
<normalizedToken id="56BC1ED95F400E860BCFFA1884B32391" originalValue="±">+/-</normalizedToken>
SD = 12.3
<normalizedToken id="B71EC6843520F2A032AABEFD82B09AE0" originalValue="±">+/-</normalizedToken>
0.6
<normalizedToken id="CF3302D30445DBC46D6A8F063BCF4E98" originalValue="×">x</normalizedToken>
8.1
<normalizedToken id="0CDC357F2E9606C09F920671D047846A" originalValue="±">+/-</normalizedToken>
0.4
<normalizedToken id="42E52512A2817FA07CA74CA8CC128097" originalValue="μm">μm</normalizedToken>
, n = 6).
</paragraph>
</subSubSection>
<subSubSection id="A751FF4C0EC04060B05A70E7259BFC14" pageId="0" pageNumber="45" type="description">
<paragraph id="A3A652FAEEA72061D652F38E58F04431" pageId="0" pageNumber="45">Culture characteristics.</paragraph>
<paragraph id="67B795E28996F43419D09B54465EC91E" pageId="0" pageNumber="45">Colonies on PDA growing in the dark with an average growth rate of 9.2 mm/d at 25 °C. Colony color white, flat, margins regular and fimbriate. Aerial mycelia abundant. Odor absent. Reverse white to pale luteous. Colonies on SNA incubated at 25 °C in the dark growing at 7.2 mm/d. Colony surface pure white, aerial mycelium scant. Reverse pure white, without diffusible pigments. Colonies on OMA incubated at 25 °C in the dark growing at 10.1 mm/d, color white, flat, velvety to felty with abundant floccose aerial mycelium. Reverse white without diffusible pigments. Colonies on CMA incubated at 25 °C in the dark were round, colony surface and reverse white, flat, aerial mycelium absent, hyphae hyaline, growing at 9.1 mm/d.</paragraph>
</subSubSection>
<subSubSection id="5E90BC7FA078E5A4C7C2353D8D8652DB" pageId="0" pageNumber="45" type="materials_examined">
<paragraph id="276CD740AC4D6A8A9310ACADA8361ADF" pageId="0" pageNumber="45">Additional materials examined.</paragraph>
<paragraph id="3A4B27DC2AF708F4D3931C85728D9F16" pageId="0" pageNumber="45">
<materialsCitation id="CB1CBB881FED3794C92E0A6DD3CF0F2F" collectingDate="2017-05" country="China" county="Yiyang City" latitude="28.456667" location="Henglongqiao Town" longLatPrecision="20" longitude="112.485275" municipality="Heshan District" specimenCount="1" stateProvince="Hunan province">
<collectingCountry id="E9802416581A9D9B39FCD560E00EBEAD" name="China">China</collectingCountry>
,
<collectingRegion id="A9BEEBC221631DA95D709B858979AB94" country="China" name="Hunan">Hunan province</collectingRegion>
,
<collectingCounty id="B5982E998B1FBFF1F338767B3800162C">Yiyang City</collectingCounty>
,
<collectingMunicipality id="A9CF9F1A6074CD551D9C5BCC3C9A4234">Heshan District</collectingMunicipality>
,
<location id="AB7121416704DF51674D9DA7572A43A0" LSID="urn:lsid:plazi:treatment:D0D7002219445506A79E318281D5606A:AB7121416704DF51674D9DA7572A43A0" country="China" county="Yiyang City" latitude="28.456667" longLatPrecision="20" longitude="112.485275" municipality="Heshan District" name="Henglongqiao Town" stateProvince="Hunan province">Henglongqiao Town</location>
,
<geoCoordinate id="4EF36122FACAB396C1DFEB4BEDC9EC0B" degrees="28" direction="north" minutes="27" orientation="latitude" precision="15" seconds="24" value="28.456667">28°2724″N</geoCoordinate>
,
<geoCoordinate id="DA00018F181D6774A0E422A5400E166E" degrees="112" direction="east" minutes="29" orientation="longitude" precision="15" seconds="7" value="112.485275">112°297″E</geoCoordinate>
, isolated from leaf spots of
<taxonomicName id="248B6B631FD9914B57776C758BD6BFA7" class="Sordariomycetes" family="Nectriaceae" genus="Cunninghamia" kingdom="Fungi" lsidName="Cunninghamia lanceolata" order="Hypocreales" pageId="0" pageNumber="45" phylum="Ascomycota" rank="species" species="lanceolata">
<emphasis id="14C5A9DE46EE8A1591F4CC2EDBF0382D" italics="true" pageId="0" pageNumber="45">Cunninghamia lanceolata</emphasis>
</taxonomicName>
,
<collectingDate id="C5332121F57F858E8A39B9343F5B3BBB" value="2017-05">May 2017</collectingDate>
, Wen-Li Cui, isolates: HN33-8-2-1, HN33-8-2-2, HN33-8-2-3
</materialsCitation>
.
</paragraph>
</subSubSection>
<subSubSection id="E0CC812C9F6158C880DBD8EC3AB2AD4E" pageId="0" pageNumber="45" type="notes">
<paragraph id="D393CA1CEA581A920671A43D5D5ED2DB" pageId="0" pageNumber="45">Notes.</paragraph>
<paragraph id="23EBB50ECBC30BA73C94E42394C532E9" pageId="0" pageNumber="45">
The isolates of
<taxonomicName id="C7490EB8167046951541A7F49D1A8BB1" lsidName="F. hunanense" pageId="0" pageNumber="45" rank="species" species="hunanense">
<emphasis id="4161DA66E72A358A1814E7F888E38482" italics="true" pageId="0" pageNumber="45">F. hunanense</emphasis>
</taxonomicName>
were phylogenetically close to
<taxonomicName id="F72CB31FD3EFA9233A72C86150118FC5" lsidName="F. pseudensiforme" pageId="0" pageNumber="45" rank="species" species="pseudensiforme">
<emphasis id="707F2A4EDA98497E09C748F619315CA8" italics="true" pageId="0" pageNumber="45">F. pseudensiforme</emphasis>
</taxonomicName>
(ex-type, CBS 125729) (Fig.
<figureCitation id="9243C4119C00127CB342E9FDBF8896EC" captionStart="Figure 4" captionStartId="F4" captionText="Figure 4. Phylogenetic relationships of 41 isolates of the Fusarium solani species complex with related taxa with concatenated sequences of the TEF- 1 α, RPB 2, and RPB 1 loci using Bayesian inference (BI) and maximum likelihood (ML) methods. Bootstrap support values from ML ≥ 70 % and BI posterior values ≥ 0.9 are shown at nodes (ML / BI). Fusarium staphyleae NRRL 22316 and F. cicatricum CBS 125552 were the outgroup. * indicates strains of this study. T indicates the ex-type strains. ET indicates ex-epitypes." figureDoi="10.3897/mycokeys.101.113128.figure4" httpUri="https://binary.pensoft.net/fig/963601" pageId="0" pageNumber="45">4</figureCitation>
). Between
<taxonomicName id="2FE9627714C82A54BE478827D6214BC1" lsidName="F. hunanense" pageId="0" pageNumber="45" rank="species" species="hunanense">
<emphasis id="C87027E6CC79DE102F22402252CD08C1" italics="true" pageId="0" pageNumber="45">F. hunanense</emphasis>
</taxonomicName>
isolates and ex-type of
<taxonomicName id="441317096EADBD850F8357622837C325" lsidName="F. pseudensiforme" pageId="0" pageNumber="45" rank="species" species="pseudensiforme">
<emphasis id="43DD99130407CF1D874D6537F083356F" italics="true" pageId="0" pageNumber="45">F. pseudensiforme</emphasis>
</taxonomicName>
CBS 125729, there were 8/583 differences in
<emphasis id="B3CB93C9817DD357C719DBE61B17CDF2" italics="true" pageId="0" pageNumber="45">TEF-1α</emphasis>
, 3/800 in
<emphasis id="CBA2ECC49588BC25B644DBE1EDAD7A19" italics="true" pageId="0" pageNumber="45">RPB2</emphasis>
, and 9/640 in
<emphasis id="2F53A8B55191E7F01CC5BCCF800D00FD" italics="true" pageId="0" pageNumber="45">RPB1</emphasis>
. The PHI analysis showed that there was no significant recombination among
<taxonomicName id="BDE549E1733C6D4116B939FB06D33A93" lsidName="F. hunanense" pageId="0" pageNumber="45" rank="species" species="hunanense">
<emphasis id="28275B24917E3AD10394B0DABE20211C" italics="true" pageId="0" pageNumber="45">F. hunanense</emphasis>
</taxonomicName>
isolates and its related species (Φw = 1.0) (Fig.
<figureCitation id="47DF91DBF2B4AE328D6953CD755EF424" captionStart="Figure 3" captionStartId="F3" captionText="Figure 3. Splitgraphs showing the results of the pairwise homoplasy index (PHI) test of three newly described taxa and closely related species using both LogDet transformation and splits decomposition A the PHI of Fusarium fujianense sp. nov. with their phylogenetically related isolates or species B the PHI of F. hunanense sp. nov. with their phylogenetically related isolates or species C the PHI of F. guizhouense sp. nov. with their phylogenetically related isolates or species. PHI test value (Φw) &lt;0.05 indicate significant recombination within a dataset. * indicates isolates of this study. T indicates ex-types. HT indicates ex-holotypes." figureDoi="10.3897/mycokeys.101.113128.figure3" httpUri="https://binary.pensoft.net/fig/963600" pageId="0" pageNumber="45">3B</figureCitation>
). Morphologically, 5-septate sporodochial macroconidia of the
<taxonomicName id="BB261763E0DB9A55311D5F0F103299FB" lsidName="F. hunanense" pageId="0" pageNumber="45" rank="species" species="hunanense">
<emphasis id="85EAAA8504B765FD8F4BCEFF8712F78D" italics="true" pageId="0" pageNumber="45">F. hunanense</emphasis>
</taxonomicName>
isolates (60.6-73.0
<normalizedToken id="7EBE76E52CAE593C940A8DA998C7B29E" originalValue="×">x</normalizedToken>
6.1-7.1
<normalizedToken id="F6E8DA3E7C70023E4D71CF94F15D566A" originalValue="µm">µm</normalizedToken>
) were longer than those of
<taxonomicName id="EE2954563C49AA02076054DAC273C1E2" lsidName="F. pseudensiforme" pageId="0" pageNumber="45" rank="species" species="pseudensiforme">
<emphasis id="596DCFC76505C57BA0C11171ABF9A5D1" italics="true" pageId="0" pageNumber="45">F. pseudensiforme</emphasis>
</taxonomicName>
CBS 125729 (ex-type) (50-63
<normalizedToken id="A886C5F0AA4AA97812A3815F8A38707C" originalValue="×">x</normalizedToken>
5.2-7.2
<normalizedToken id="C8D9BB0EE82536C0155604E73C4E92F3" originalValue="µm">µm</normalizedToken>
) (
<bibRefCitation id="649B413E7E6D279B10BD3103212448D6" DOI="https://doi.org/10.3852/10-307" author="Nalim, FA" journalOrPublisher="Mycologia" pageId="0" pageNumber="45" pagination="1302 - 1330" refId="B50" refString="Nalim, FA, Samuels, GJ, Wijesundera, RL, Geiser, DM, 2011. New species from the Fusarium solani species complex derived from perithecia and soil in the old world tropics. Mycologia 103 (6): 1302 - 1330, DOI: https://doi.org/10.3852/10-307" title="New species from the Fusarium solani species complex derived from perithecia and soil in the old world tropics." url="https://doi.org/10.3852/10-307" volume="103" year="2011">Nalim et al. 2011</bibRefCitation>
). In conclusion, the phylogenetic and morphological evidence supported this fungus being a new species within the
<taxonomicName id="87D16E6031DE7713546DAE69861EBE91" lsidName="F. solani" pageId="0" pageNumber="45" rank="species" species="solani">
<emphasis id="97AF8543B13EA929F0C1E815375EB45D" italics="true" pageId="0" pageNumber="45">F. solani</emphasis>
</taxonomicName>
species complex.
</paragraph>
</subSubSection>
<subSubSection id="223A4B3DE982DC00FEB6E9604CC91A6A" pageId="0" pageNumber="45" type="pathogenicity assays">
<paragraph id="23D112548104DE4A0306EF17236C1772" pageId="0" pageNumber="45">Pathogenicity assays.</paragraph>
<paragraph id="F1139106F422DC1A7682B9D91F348293" pageId="0" pageNumber="45">
Pathogenicity was tested on detached
<taxonomicName id="057E287D8541E154C44ADDD369B8FA16" lsidName="C. lanceolata" pageId="0" pageNumber="45" rank="species" species="lanceolata">
<emphasis id="A72BAD5B522CD10AF799D4A0333F1545" italics="true" pageId="0" pageNumber="45">C. lanceolata</emphasis>
</taxonomicName>
leaves
<emphasis id="68AFD82DAB3D485BFA2A800D1306D447" italics="true" pageId="0" pageNumber="45">in vitro</emphasis>
following
<normalizedToken id="45B0F89AA2EF18B25A5EC3DB6637085C" originalValue="Kochs">Koch's</normalizedToken>
postulates for
<taxonomicName id="EC1986756869F06A23C851B34AC85B15" lsidName="F. hunanense" pageId="0" pageNumber="45" rank="species" species="hunanense">
<emphasis id="0C671B3DE5DCB9F8BA412D81E4528A8C" italics="true" pageId="0" pageNumber="45">F. hunanense</emphasis>
</taxonomicName>
(HN33-8-2),
<taxonomicName id="23128EDF676C418AFD68A9E46CAA3A57" lsidName="F. concentricum" pageId="0" pageNumber="45" rank="species" species="concentricum">
<emphasis id="C41AAD91C9C1CD305C3A16E848D6D51B" italics="true" pageId="0" pageNumber="45">F. concentricum</emphasis>
</taxonomicName>
(SJ1-10),
<taxonomicName id="74474246F04F56F6A1F5DE649F630141" lsidName="F. guizhouense" pageId="0" pageNumber="45" rank="species" species="guizhouense">
<emphasis id="93F4FA4439356A167ED1E7720189A345" italics="true" pageId="0" pageNumber="45">F. guizhouense</emphasis>
</taxonomicName>
(GZ7-20-1),
<taxonomicName id="F94854D7F3C69FF2726D713A9164988B" lsidName="F. fujikuroi" pageId="0" pageNumber="45" rank="species" species="fujikuroi">
<emphasis id="FE9126AC442D46085EC0F8EEDFA739C3" italics="true" pageId="0" pageNumber="45">F. fujikuroi</emphasis>
</taxonomicName>
(HN43-17-1), and
<taxonomicName id="ACCB7924C0636AD361FE79B252AC4D3B" lsidName="F. fujianense" pageId="0" pageNumber="45" rank="species" species="fujianense">
<emphasis id="8BC8D18B111C780FE902F405055B6093" italics="true" pageId="0" pageNumber="45">F. fujianense</emphasis>
</taxonomicName>
(LC14). At five days post-inoculation, all the tested isolates caused leaf necrosis, with dark brown lesions. The control remained unchanged (Fig.
<figureCitation id="DBB5F6E2BCC5714CBBF4D7BDB0AC3802" captionStart="Figure 9" captionStartId="F9" captionText="Figure 9. Symptoms on detached Cunninghamia lanceolata leaves (A) and shoots of tissue-culture seedlings of C. lanceolata (B) inoculated with isolates: Fusarium fujianense (LC 14), F. fujikuroi (HN 43 - 17 - 1), F. guizhouense (GZ 7 - 20 - 1), F. concentricum (SJ 1 - 10), and F. hunanense (HN 33 - 8 - 2). Scale bar: 10 mm. C, Lesion length on detached C. lanceolata leaves inoculated with F. fujianense (LC 14), F. fujikuroi (HN 43 - 17 - 1), F. guizhouense (GZ 7 - 20 - 1), F. concentricum (SJ 1 - 10), and F. hunanense (HN 33 - 8 - 2). Error bars represent standard deviation, and different letters indicate significant difference based on LSD's range test at P &lt;0.05 (n = 8)." figureDoi="10.3897/mycokeys.101.113128.figure9" httpUri="https://binary.pensoft.net/fig/963606" pageId="0" pageNumber="45">9A</figureCitation>
). Equivalently, shoots of tissue-culture seedlings of
<taxonomicName id="BAD6B3C3BD4121A136003D7A87B2A2EE" lsidName="C. lanceolata" pageId="0" pageNumber="45" rank="species" species="lanceolata">
<emphasis id="1BF225404AA5C0A539696117CBFD7CF1" italics="true" pageId="0" pageNumber="45">C. lanceolata</emphasis>
</taxonomicName>
were inoculated by
<taxonomicName id="1A33FCAAA7B6C2F673E870B76491E31A" lsidName="F. hunanense" pageId="0" pageNumber="45" rank="species" species="hunanense">
<emphasis id="E087ECDAE60B5DDA3366846BB70D2F2D" italics="true" pageId="0" pageNumber="45">F. hunanense</emphasis>
</taxonomicName>
(HN33-8-2),
<taxonomicName id="B4D1789990AAAF1D28BF2E0BD59F0657" lsidName="F. concentricum" pageId="0" pageNumber="45" rank="species" species="concentricum">
<emphasis id="6441F056EF2447EF72013B2397B61B9E" italics="true" pageId="0" pageNumber="45">F. concentricum</emphasis>
</taxonomicName>
(SJ1-10),
<taxonomicName id="604B9480D202327A929738BDEC0A9660" lsidName="F. guizhouense" pageId="0" pageNumber="45" rank="species" species="guizhouense">
<emphasis id="6D07E0E6E6A48018CD01C2E1362C9D69" italics="true" pageId="0" pageNumber="45">F. guizhouense</emphasis>
</taxonomicName>
(GZ7-20-1),
<taxonomicName id="63664BC94D853442FF5E99F24E80E4CC" lsidName="F. fujikuroi" pageId="0" pageNumber="45" rank="species" species="fujikuroi">
<emphasis id="F23119A77D7552EC7949902B1A2C1371" italics="true" pageId="0" pageNumber="45">F. fujikuroi</emphasis>
</taxonomicName>
(HN43-17-1), and
<taxonomicName id="DA90BFACBA2FCE6BB78013A4C99DF797" lsidName="F. fujianense" pageId="0" pageNumber="45" rank="species" species="fujianense">
<emphasis id="BAC84DB811C6C50B0938842B872D2DA7" italics="true" pageId="0" pageNumber="45">F. fujianense</emphasis>
</taxonomicName>
(LC14)
<emphasis id="44CD4B541D229CCD460C87627996A74E" italics="true" pageId="0" pageNumber="45">in vivo</emphasis>
. After ten days post-inoculation, all isolates caused necrotic lesions on shoots of
<taxonomicName id="2CF18BA4C7EECDD8A5B3EC8A7A9C280A" lsidName="C. lanceolata" pageId="0" pageNumber="45" rank="species" species="lanceolata">
<emphasis id="8567838F02802290E18898A0016182AB" italics="true" pageId="0" pageNumber="45">C. lanceolata</emphasis>
</taxonomicName>
. The control remained healthy (Fig.
<figureCitation id="7D371E5D33D3EBE1DEC3497DC3A06E6A" captionStart="Figure 9" captionStartId="F9" captionText="Figure 9. Symptoms on detached Cunninghamia lanceolata leaves (A) and shoots of tissue-culture seedlings of C. lanceolata (B) inoculated with isolates: Fusarium fujianense (LC 14), F. fujikuroi (HN 43 - 17 - 1), F. guizhouense (GZ 7 - 20 - 1), F. concentricum (SJ 1 - 10), and F. hunanense (HN 33 - 8 - 2). Scale bar: 10 mm. C, Lesion length on detached C. lanceolata leaves inoculated with F. fujianense (LC 14), F. fujikuroi (HN 43 - 17 - 1), F. guizhouense (GZ 7 - 20 - 1), F. concentricum (SJ 1 - 10), and F. hunanense (HN 33 - 8 - 2). Error bars represent standard deviation, and different letters indicate significant difference based on LSD's range test at P &lt;0.05 (n = 8)." figureDoi="10.3897/mycokeys.101.113128.figure9" httpUri="https://binary.pensoft.net/fig/963606" pageId="0" pageNumber="45">9B</figureCitation>
). Statistically, these isolates showed different levels of virulence.
<taxonomicName id="ADB3E236D23AD39106752826E41BFCBE" authorityName="Lin Huang, Jiao He &amp; D. W. Li" authorityYear="2024" class="Ascomycetes" family="Nectriaceae" genus="Fusarium" kingdom="Fungi" lsidName="Fusarium hunanense" order="Hypocreales" pageId="0" pageNumber="45" phylum="Ascomycota" rank="species" species="hunanense">
<emphasis id="87A9116D9036845549FF47ACFF17C725" italics="true" pageId="0" pageNumber="45">Fusarium hunanense</emphasis>
</taxonomicName>
(HN33-8-2) was significantly more virulent than those of
<taxonomicName id="FBD803BF2127811D52590B922F9AAAAB" lsidName="F. concentricum" pageId="0" pageNumber="45" rank="species" species="concentricum">
<emphasis id="1914224FB785F95E8E8697BAC1298101" italics="true" pageId="0" pageNumber="45">F. concentricum</emphasis>
</taxonomicName>
(SJ1-10),
<taxonomicName id="5C3D17836D55D623DEFDFE753C77E887" lsidName="F. guizhouense" pageId="0" pageNumber="45" rank="species" species="guizhouense">
<emphasis id="05A17A0AA45EB70F546502998FD1703E" italics="true" pageId="0" pageNumber="45">F. guizhouense</emphasis>
</taxonomicName>
(GZ7-20-1),
<taxonomicName id="AE470F951B27541062C0390DABA01489" lsidName="F. fujikuroi" pageId="0" pageNumber="45" rank="species" species="fujikuroi">
<emphasis id="68BE494E764B33954B35B7B11ABE0E01" italics="true" pageId="0" pageNumber="45">F. fujikuroi</emphasis>
</taxonomicName>
(HN43-17-1), and
<taxonomicName id="4BAE8F85B12A9162B0FBA9A74B3657B1" lsidName="F. fujianense" pageId="0" pageNumber="45" rank="species" species="fujianense">
<emphasis id="BD9A1C5891746A5BE3E766216501F3B7" italics="true" pageId="0" pageNumber="45">F. fujianense</emphasis>
</taxonomicName>
(LC14), while
<taxonomicName id="B0874133D4E48B0FA0237D19CE8363CF" lsidName="F. fujianense" pageId="0" pageNumber="45" rank="species" species="fujianense">
<emphasis id="D0E3168A5B3152305CA757FC78BB49E7" italics="true" pageId="0" pageNumber="45">F. fujianense</emphasis>
</taxonomicName>
(LC14) was the least virulent (Fig.
<figureCitation id="BB8E0307A797B07565C2B284DBD363F3" captionStart="Figure 9" captionStartId="F9" captionText="Figure 9. Symptoms on detached Cunninghamia lanceolata leaves (A) and shoots of tissue-culture seedlings of C. lanceolata (B) inoculated with isolates: Fusarium fujianense (LC 14), F. fujikuroi (HN 43 - 17 - 1), F. guizhouense (GZ 7 - 20 - 1), F. concentricum (SJ 1 - 10), and F. hunanense (HN 33 - 8 - 2). Scale bar: 10 mm. C, Lesion length on detached C. lanceolata leaves inoculated with F. fujianense (LC 14), F. fujikuroi (HN 43 - 17 - 1), F. guizhouense (GZ 7 - 20 - 1), F. concentricum (SJ 1 - 10), and F. hunanense (HN 33 - 8 - 2). Error bars represent standard deviation, and different letters indicate significant difference based on LSD's range test at P &lt;0.05 (n = 8)." figureDoi="10.3897/mycokeys.101.113128.figure9" httpUri="https://binary.pensoft.net/fig/963606" pageId="0" pageNumber="45">9C</figureCitation>
).
</paragraph>
<caption id="77DD8BEE6B4F9BACA8E5EFD188A53C02" doi="10.3897/mycokeys.101.113128.figure9" httpUri="https://binary.pensoft.net/fig/963606" pageId="0" pageNumber="45" start="Figure 9" startId="F9">
<paragraph id="4C449EB118AAF2EFA8EE6C4066E8FE8D" pageId="0" pageNumber="45">
<emphasis id="E895A0D241A922ACED7AC516F8237860" bold="true" pageId="0" pageNumber="45">Figure 9.</emphasis>
Symptoms on detached
<taxonomicName id="FBB3B1191A70FB29BE6F4E73BDD4D977" class="Sordariomycetes" family="Nectriaceae" genus="Cunninghamia" kingdom="Fungi" lsidName="Cunninghamia lanceolata" order="Hypocreales" pageId="0" pageNumber="45" phylum="Ascomycota" rank="species" species="lanceolata">
<emphasis id="6695BA4D5812C943DF835B5F45A908E9" italics="true" pageId="0" pageNumber="45">Cunninghamia lanceolata</emphasis>
</taxonomicName>
leaves (
<emphasis id="17B85CCCAAF06C9A311902D715C2E94B" bold="true" pageId="0" pageNumber="45">A</emphasis>
) and shoots of tissue-culture seedlings of
<taxonomicName id="33FBC89A7D75640083A9E93AA10D5BF4" lsidName="C. lanceolata" pageId="0" pageNumber="45" rank="species" species="lanceolata">
<emphasis id="AC05446773981E99DF407103CFB03708" italics="true" pageId="0" pageNumber="45">C. lanceolata</emphasis>
</taxonomicName>
(
<emphasis id="A6CC1054F9CA663BDF9B33B327D7DCB4" bold="true" pageId="0" pageNumber="45">B</emphasis>
) inoculated with isolates:
<taxonomicName id="88C031C7668FEBA2EC0622713CDB7858" authorityName="Lin Huang, Jiao He &amp; D. W. Li" authorityYear="2024" class="Ascomycetes" family="Nectriaceae" genus="Fusarium" kingdom="Fungi" lsidName="Fusarium fujianense" order="Hypocreales" pageId="0" pageNumber="45" phylum="Ascomycota" rank="species" species="fujianense">
<emphasis id="832B1CD9F9429D2AE0DE849CBE70E9A4" italics="true" pageId="0" pageNumber="45">Fusarium fujianense</emphasis>
</taxonomicName>
(LC14),
<taxonomicName id="5C286069ABE62B664F1899F3B8E224D8" lsidName="F. fujikuroi" pageId="0" pageNumber="45" rank="species" species="fujikuroi">
<emphasis id="49368A497A726BF1363ADC4EE69123E5" italics="true" pageId="0" pageNumber="45">F. fujikuroi</emphasis>
</taxonomicName>
(HN43-17-1),
<taxonomicName id="DD6570A685D7AA98DF5294ABD31530C0" lsidName="F. guizhouense" pageId="0" pageNumber="45" rank="species" species="guizhouense">
<emphasis id="4200ECDE557EE74D0B23BB8641AE4C94" italics="true" pageId="0" pageNumber="45">F. guizhouense</emphasis>
</taxonomicName>
(GZ7-20-1),
<taxonomicName id="B2DF752E8FD9B9FCF66CD65AEE979CA5" lsidName="F. concentricum" pageId="0" pageNumber="45" rank="species" species="concentricum">
<emphasis id="76FF8414D83AD4370240D803F2B095EC" italics="true" pageId="0" pageNumber="45">F. concentricum</emphasis>
</taxonomicName>
(SJ1-10), and
<taxonomicName id="68CD442417B7C3B1DABCB3392399CE21" lsidName="F. hunanense" pageId="0" pageNumber="45" rank="species" species="hunanense">
<emphasis id="6D7BF10CD6DDE25469D057C0235C3FD3" italics="true" pageId="0" pageNumber="45">F. hunanense</emphasis>
</taxonomicName>
(HN33-8-2). Scale bar: 10 mm. C, Lesion length on detached
<taxonomicName id="CEB213788BE3F01F32D8D7F200CE595F" lsidName="C. lanceolata" pageId="0" pageNumber="45" rank="species" species="lanceolata">
<emphasis id="9DD349A42A1A859DA515BBB611852226" italics="true" pageId="0" pageNumber="45">C. lanceolata</emphasis>
</taxonomicName>
leaves inoculated with
<taxonomicName id="3DE04A3D7AC787DFF2369697EDDF57A1" lsidName="F. fujianense" pageId="0" pageNumber="45" rank="species" species="fujianense">
<emphasis id="B3B9C0909E2EED0CEEB1AF909D3C23FD" italics="true" pageId="0" pageNumber="45">F. fujianense</emphasis>
</taxonomicName>
(LC14),
<taxonomicName id="48FB0707A31592A63ED238A3AED9CACD" lsidName="F. fujikuroi" pageId="0" pageNumber="45" rank="species" species="fujikuroi">
<emphasis id="177C6274D2F69641F4073E68D8071FFB" italics="true" pageId="0" pageNumber="45">F. fujikuroi</emphasis>
</taxonomicName>
(HN43-17-1),
<taxonomicName id="547BDB44951E332FCFCDD5EC88A48DF5" lsidName="F. guizhouense" pageId="0" pageNumber="45" rank="species" species="guizhouense">
<emphasis id="404468038CFBFE33C9A3EC82150CB593" italics="true" pageId="0" pageNumber="45">F. guizhouense</emphasis>
</taxonomicName>
(GZ7-20-1),
<taxonomicName id="982B3B94651F4024450D30A3867152E3" lsidName="F. concentricum" pageId="0" pageNumber="45" rank="species" species="concentricum">
<emphasis id="4F579D11F78BEED4B8953601FA498757" italics="true" pageId="0" pageNumber="45">F. concentricum</emphasis>
</taxonomicName>
(SJ1-10), and
<taxonomicName id="09B02526EE969A08F7E6164C602C2CA0" lsidName="F. hunanense" pageId="0" pageNumber="45" rank="species" species="hunanense">
<emphasis id="74575CB357C4E9BD64D8F2A812C4663D" italics="true" pageId="0" pageNumber="45">F. hunanense</emphasis>
</taxonomicName>
(HN33-8-2). Error bars represent standard deviation, and different letters indicate significant difference based on
<normalizedToken id="2E046D3CC5D30B13F57C1439E278D8B2" originalValue="LSDs">LSD's</normalizedToken>
range test at
<emphasis id="9A8AA258B0AE2FDACCB01E8D61BCE9EE" italics="true" pageId="0" pageNumber="45">P</emphasis>
&lt;0.05 (n = 8).
</paragraph>
</caption>
<paragraph id="B00778112892BE24C4B5C5B681F02704" pageId="0" pageNumber="45">
The fungal isolates used for inoculation were re-isolated from the diseased spots on the inoculated leaves and shoots, but no fungus was isolated from the leaves and shoots of the control.
<normalizedToken id="024C7D62FF4A04A6AB804E56E65B9C04" originalValue="Kochs">Koch's</normalizedToken>
postulates were satisfied, and these isolates HN33-8-2, SJ1-10, GZ7-20-1, HN43-17-1, and LC14 were determined to be the pathogens of leaf blight on
<taxonomicName id="463C4E7BEF66BDC669263879237AEF78" lsidName="C. lanceolata" pageId="0" pageNumber="45" rank="species" species="lanceolata">
<emphasis id="E015C5575FD15593633830ACE60D35AC" italics="true" pageId="0" pageNumber="45">C. lanceolata</emphasis>
</taxonomicName>
.
</paragraph>
</subSubSection>
</treatment>
</subSection>
</document>