288 lines
31 KiB
XML
288 lines
31 KiB
XML
<document id="D0A008DD20C65B0ACE5D172B1854D949" ID-DOI="10.11646/phytotaxa.653.1.2" ID-ISSN="1179-3163" ID-Zenodo-Dep="13216863" IM.bibliography_approvedBy="carolina" IM.illustrations_approvedBy="carolina" IM.materialsCitations_approvedBy="carolina" IM.tables_approvedBy="carolina" IM.taxonomicNames_approvedBy="carolina" IM.treatments_approvedBy="carolina" checkinTime="1722603289460" checkinUser="felipe" docAuthor="Gong, Cui-Ling, Wu, Hai-Xia, Li, Jin-Chen & Song, Jia-Yu" docDate="2024" docId="03BE879BFFB8C812D4A1F8DAFBCF545A" docLanguage="en" docName="phytotaxa.653.1.2.pdf" docOrigin="Phytotaxa 653 (1)" docSource="http://dx.doi.org/10.11646/phytotaxa.653.1.2" docStyle="DocumentStyle:96748F8F1B6C902996E134952A3A36B9.13:Phytotaxa.2014-.journal_article" docStyleId="96748F8F1B6C902996E134952A3A36B9" docStyleName="Phytotaxa.2014-.journal_article" docStyleVersion="13" docTitle="Phyllosticta savannaensis H. X. Wu & C. L. Gong., sp. nov." docType="treatment" docVersion="1" lastPageNumber="25" masterDocId="FF87FFE3FFBAC817D429FFEEFF8F5375" masterDocTitle="Morphological and multi-gene phylogenetic analyses of two new species of Phyllosticta (Phyllostictaceae, Botryosphaeriales) from Southwestern China" masterLastPageNumber="36" masterPageNumber="20" pageNumber="22" updateTime="1724884690337" updateUser="carolina" zenodo-license-figures="UNSPECIFIED" zenodo-license-treatments="UNSPECIFIED">
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<mods:title id="7356BC4C6B6121B2177F98C49C7B924E">Morphological and multi-gene phylogenetic analyses of two new species of Phyllosticta (Phyllostictaceae, Botryosphaeriales) from Southwestern China</mods:title>
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<mods:namePart id="61C97FFAA027D6487316E6C23CE986FA">Gong, Cui-Ling</mods:namePart>
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<mods:affiliation id="470A4DDCC81D7656DF69EB1DB039CA3D">International Fungal Research and Development Centre, Institute of Highland Forest Science, Chinese Academy of Forestry, Kunming 650224, Yunnan, P. R. of China. & Nanjing Forestry University, Nanjing 210037, P. R. of China.</mods:affiliation>
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<mods:namePart id="ACCA4414E0AD6573EC92E29404D2F8A0">Wu, Hai-Xia</mods:namePart>
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<mods:affiliation id="7E80C0BFE96783EC43F669FCF403FEEE">International Fungal Research and Development Centre, Institute of Highland Forest Science, Chinese Academy of Forestry, Kunming 650224, Yunnan, P. R. of China.</mods:affiliation>
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<mods:nameIdentifier id="79E6365190E515854326E07FDCA70F28" type="email">aileen2008haixia@gmail.com</mods:nameIdentifier>
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<mods:namePart id="87F7DE662F67F43FB71531084EDE4AB5">Li, Jin-Chen</mods:namePart>
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<mods:affiliation id="1C7E2F4A4408EC8AEB4658EF76AB8B1D">International Fungal Research and Development Centre, Institute of Highland Forest Science, Chinese Academy of Forestry, Kunming 650224, Yunnan, P. R. of China.</mods:affiliation>
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<mods:nameIdentifier id="7D5E10FC532C26B60404C26A4A62FB58" type="email">jinchenlii@163.com</mods:nameIdentifier>
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<mods:namePart id="CBF20E45491AA09CCEED25004801109D">Song, Jia-Yu</mods:namePart>
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<mods:affiliation id="C97485A769CAD71179B710AE053F9685">International Fungal Research and Development Centre, Institute of Highland Forest Science, Chinese Academy of Forestry, Kunming 650224, Yunnan, P. R. of China.</mods:affiliation>
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<mods:nameIdentifier id="70CCE52A9521419C5BB9F111B002BAE2" type="email">ldsat-f@outlook.com</mods:nameIdentifier>
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<mods:title id="45E25503CB23DD4E11D389DA3601D758">Phytotaxa</mods:title>
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<mods:part id="650B533AD510BAEB7DF002065FD6F2A2">
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<mods:date id="C6718B2A0424435CE5465381756FF816">2024</mods:date>
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<mods:number id="87244DF3F5D88D954DB4587C0D2ABC34">2024-06-13</mods:number>
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<mods:detail id="5A054EB215BAAE548C700789D58A66F0" type="volume">
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<mods:number id="5C48174D328BC7EBDDA3026F8DDB5A61">653</mods:number>
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<mods:start id="4DF95AD0752635855A3A5A5D86F8D8E4">20</mods:start>
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<mods:url id="2EAFA967E58771BB46A731172FB32AA8">http://dx.doi.org/10.11646/phytotaxa.653.1.2</mods:url>
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<mods:classification id="F7BC719F815325EA80A45F5DE93278F5">journal article</mods:classification>
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<mods:identifier id="0F2F44FF0A477602E3D80742AAF22B47" type="DOI">10.11646/phytotaxa.653.1.2</mods:identifier>
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<mods:identifier id="B52CA1270016CDCB6A3420B9FD200A33" type="ISSN">1179-3163</mods:identifier>
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<mods:identifier id="9C6C402C6D87EB656E6A2D897D3A2742" type="Zenodo-Dep">13216863</mods:identifier>
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<treatment id="03BE879BFFB8C812D4A1F8DAFBCF545A" LSID="urn:lsid:plazi:treatment:03BE879BFFB8C812D4A1F8DAFBCF545A" httpUri="http://treatment.plazi.org/id/03BE879BFFB8C812D4A1F8DAFBCF545A" lastPageId="5" lastPageNumber="25" pageId="2" pageNumber="22">
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<subSubSection id="C30D6506FFB8C815D4A1F8DAFCEE543B" box="[136,865,1844,1871]" pageId="2" pageNumber="22" type="nomenclature">
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<paragraph id="8BA8368DFFB8C815D4A1F8DAFCEE543B" blockId="2.[136,865,1844,1871]" box="[136,865,1844,1871]" pageId="2" pageNumber="22">
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<heading id="D0E081E1FFB8C815D4A1F8DAFCEE543B" box="[136,865,1844,1871]" fontSize="11" level="2" pageId="2" pageNumber="22" reason="3">
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<taxonomicName id="4C174D0EFFB8C815D4A1F8DAFD31543B" authority="H.X. Wu & C.L. Gong." authorityName="H. X. Wu & C. L. Gong." box="[136,702,1844,1871]" class="Sordariomycetes" family="Diaporthaceae" genus="Phyllosticta" kingdom="Fungi" order="Diaporthales" pageId="2" pageNumber="22" phylum="Ascomycota" rank="species" species="savannaensis" status="sp. nov.">
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<emphasis id="B963EA9FFFB8C815D4A1F8DAFE3F543B" bold="true" box="[136,432,1844,1871]" italics="true" pageId="2" pageNumber="22">Phyllosticta savannaensis</emphasis>
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H.X. Wu & C.L. Gong.
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</taxonomicName>
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<emphasis id="B963EA9FFFB8C815D6EDF8DBFC96543B" box="[708,793,1845,1870]" italics="true" pageId="2" pageNumber="22">
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<taxonomicNameLabel id="A25057E4FFB8C815D6EDF8DBFC96543B" box="[708,793,1845,1870]" pageId="2" pageNumber="22" rank="species">sp. nov.</taxonomicNameLabel>
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</emphasis>
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<figureCitation id="132C2A08FFB8C815D709F8DAFCEE543B" box="[800,865,1844,1870]" captionStart="FIGURE 2" captionStartId="5.[136,229,1168,1190]" captionTargetBox="[413,1178,239,1097]" captionTargetId="figure-22@5.[410,1178,236,1100]" captionTargetPageId="5" captionText="FIGURE 2. Phyllosticta savannaensis (IFRD 9476, holotype). a. Microfungi on leaf litter. b–c. Ascomata on leaf surface. d–e. Vertical section of ascomata. f–i. Asci (i in cotton blue). j–n. Ascospores (m, n in cotton blue). Scale bars: b = 1000 μm, c= 500 μm, d = 100 μm, e, g–i = 30 μm, f = 50 μm, j–n = 10 μm." figureDoi="http://doi.org/10.5281/zenodo.13216867" httpUri="https://zenodo.org/record/13216867/files/figure.png" pageId="2" pageNumber="22">Fig. 2</figureCitation>
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</heading>
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</paragraph>
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</subSubSection>
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<subSubSection id="C30D6506FFB8C815D4A1F8B2FE035407" box="[136,396,1884,1906]" pageId="2" pageNumber="22" type="description">
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<paragraph id="8BA8368DFFB8C815D4A1F8B2FE035407" blockId="2.[136,1400,1884,1978]" box="[136,396,1884,1906]" pageId="2" pageNumber="22">Fungal Names: FN 571911</paragraph>
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</subSubSection>
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<subSubSection id="C30D6506FFB8C815D4A1F86EFEE554CF" pageId="2" pageNumber="22" type="etymology">
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<paragraph id="8BA8368DFFB8C815D4A1F86EFEE554CF" blockId="2.[136,1400,1884,1978]" pageId="2" pageNumber="22">
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<emphasis id="B963EA9FFFB8C815D4A1F86EFE8154E0" bold="true" box="[136,270,1920,1941]" pageId="2" pageNumber="22">Etymology—</emphasis>
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<taxonomicName id="4C174D0EFFB8C815D526F86EFE0054E0" authority="Savanna" authorityName="Savanna" box="[271,399,1920,1941]" class="Sordariomycetes" family="Diaporthaceae" genus="Phyllosticta" kingdom="Fungi" order="Diaporthales" pageId="2" pageNumber="22" phylum="Ascomycota" rank="species" species="savannaensis">
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<emphasis id="B963EA9FFFB8C815D526F86EFE0054E0" box="[271,399,1920,1941]" italics="true" pageId="2" pageNumber="22">savannaensis</emphasis>
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</taxonomicName>
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= Savanna, in reference to its special location,
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<collectingRegion id="49D3F86FFFB8C815D77DF86EFC1254E0" box="[852,925,1920,1941]" country="China" name="Yunnan" pageId="2" pageNumber="22">Yunnan</collectingRegion>
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Yuanjiang Savanna Ecosystem Research Station.
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<emphasis id="B963EA9FFFB8C815D4A1F84AFF7254CC" bold="true" box="[136,253,1956,1977]" pageId="2" pageNumber="22">
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<typeStatus id="54AC882FFFB8C815D4A1F84AFF6A54CC" box="[136,229,1956,1977]" pageId="2" pageNumber="22" type="holotype">Holotype</typeStatus>
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—
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</emphasis>
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IFRD 9476
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</paragraph>
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</subSubSection>
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<subSubSection id="C30D6506FFB8C814D4A1F806FE6452E5" lastPageId="3" lastPageNumber="23" pageId="2" pageNumber="22" type="description">
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<paragraph id="8BA8368DFFB8C814D4A1F806FE6452E5" blockId="2.[136,1451,2024,2087]" lastBlockId="3.[136,1452,158,401]" lastPageId="3" lastPageNumber="23" pageId="2" pageNumber="22">
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Saprobic on leaf litter.
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<emphasis id="B963EA9FFFB8C815D5A1F806FDBF5B77" bold="true" box="[392,560,2024,2050]" pageId="2" pageNumber="22">Sexual morph</emphasis>
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: Black ascomata observed on leaf surface. Ascomata 95–130 μm (
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<emphasis id="B963EA9FFFB8C815D137F802FAA45489" box="[1310,1323,2028,2044]" italics="true" pageId="2" pageNumber="22">x</emphasis>
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= 109 μm, n = 20) in diameter, semi-buried, globose, black, carbonaceous, releasing ascospores at maturity by dehiscence and forming a central ostiole. Peridium thin, comprising a few layers of angular cells.Asci 40–70 × 10–20 μm (
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<emphasis id="B963EA9FFFB9C814D103FF4CFAB853C7" box="[1322,1335,162,178]" italics="true" pageId="3" pageNumber="23">x</emphasis>
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= 52 × 15 μm, n = 20), 8-spored, bitunicate, clavate to broadly ellipsoid, with a broad, gradually tapering pedicel, with an apical ring and a chamber diameter of 3 μm. Ascospores 12–17 × 5–7 μm (
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<emphasis id="B963EA9FFFB9C814D7B8FF04FC11538F" box="[913,926,234,250]" italics="true" pageId="3" pageNumber="23">x</emphasis>
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= 15 × 6 μm, n = 20), ratio of length to width is 2.43:1, biseriate, limoniform with obtuse ends, widest in the centre, hyaline, smooth, and multiguttulate or a large central guttule, occasionally with gelatinous appendages which are more obvious in cotton blue, apical appendage hatshaped, bottom appendage flame-like. Mucilaginous caps not present once released (appendages shown by arrows).
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<emphasis id="B963EA9FFFB9C814D4A1FE98FEB052E5" bold="true" box="[136,319,374,400]" pageId="3" pageNumber="23">Asexual morph</emphasis>
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: Not observed.
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</paragraph>
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</subSubSection>
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<caption id="DF686605FFB9C814D4A1F8CEFF6854D7" ID-DOI="http://doi.org/10.5281/zenodo.13216865" ID-Zenodo-Dep="13216865" httpUri="https://zenodo.org/record/13216865/files/figure.png" pageId="3" pageNumber="23" startId="3.[136,229,1824,1846]" targetBox="[151,1435,422,1796]" targetPageId="3" targetType="figure">
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<paragraph id="8BA8368DFFB9C814D4A1F8CEFF6854D7" blockId="3.[136,1452,1824,1954]" pageId="3" pageNumber="23">
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<emphasis id="B963EA9FFFB9C814D4A1F8CEFE8F5440" bold="true" box="[136,256,1824,1846]" pageId="3" pageNumber="23">FIGURE 1.</emphasis>
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Phylogram of
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<taxonomicName id="4C174D0EFFB9C814D5BEF8CEFD855440" box="[407,522,1824,1845]" class="Sordariomycetes" family="Diaporthaceae" genus="Phyllosticta" kingdom="Fungi" order="Diaporthales" pageId="3" pageNumber="23" phylum="Ascomycota" rank="genus">
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<emphasis id="B963EA9FFFB9C814D5BEF8CEFD855440" box="[407,522,1824,1845]" italics="true" pageId="3" pageNumber="23">Phyllosticta</emphasis>
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</taxonomicName>
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resulting from a maximum likelihood analysis based on a combined matrix of ITS, LSU and
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<emphasis id="B963EA9FFFB9C814D4A1F8AAFF30542C" box="[136,191,1860,1881]" italics="true" pageId="3" pageNumber="23">TEF1</emphasis>
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loci. The tree is artificially rooted to
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<taxonomicName id="4C174D0EFFB9C814D618F8ABFD01542C" box="[561,654,1860,1882]" class="Sordariomycetes" family="Botryosphaeriaceae" genus="Botryosphaeria" higherTaxonomySource="CoL" kingdom="Fungi" order="Diaporthales" pageId="3" pageNumber="23" phylum="Ascomycota" rank="species" species="obtusa">
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<emphasis id="B963EA9FFFB9C814D618F8ABFD01542C" box="[561,654,1860,1882]" italics="true" pageId="3" pageNumber="23">B. obtusa</emphasis>
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</taxonomicName>
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(CMW 8232). ML bootstrap values (left, ML-BS ≥ 50%) and Bayesian posterior probabilities (right, BYPP ≥ 0.9) are given at the nodes. New species of this study are indicated using red font and their associated support is bolded.
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</paragraph>
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</caption>
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<caption id="DF686605FFBEC813D4A1F89AFEF854FF" box="[136,375,1908,1930]" isContinuationCaption="true" pageId="4" pageNumber="24" startId="4.[136,229,1908,1930]" targetBox="[151,1435,190,1879]" targetPageId="4" targetType="figure">
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<paragraph id="8BA8368DFFBEC813D4A1F89AFEF854FF" blockId="4.[136,375,1908,1930]" box="[136,375,1908,1930]" pageId="4" pageNumber="24">
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<emphasis id="B963EA9FFFBEC813D4A1F89AFF7154FC" bold="true" box="[136,254,1908,1930]" pageId="4" pageNumber="24">FIGURE 1.</emphasis>
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(Continued)
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</paragraph>
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</caption>
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<caption id="DF686605FFBFC812D4A1FB7EFD875798" ID-DOI="http://doi.org/10.5281/zenodo.13216867" ID-Zenodo-Dep="13216867" httpUri="https://zenodo.org/record/13216867/files/figure.png" pageId="5" pageNumber="25" startId="5.[136,229,1168,1190]" targetBox="[413,1178,239,1097]" targetPageId="5" targetType="figure">
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<paragraph id="8BA8368DFFBFC812D4A1FB7EFD875798" blockId="5.[136,1452,1167,1262]" pageId="5" pageNumber="25">
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<emphasis id="B963EA9FFFBFC812D4A1FB7EFD8B57D0" bold="true" box="[136,516,1167,1190]" pageId="5" pageNumber="25">
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FIGURE 2.
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<taxonomicName id="4C174D0EFFBFC812D52CFB61FD8B57D0" box="[261,516,1167,1189]" class="Sordariomycetes" family="Diaporthaceae" genus="Phyllosticta" kingdom="Fungi" order="Diaporthales" pageId="5" pageNumber="25" phylum="Ascomycota" rank="species" species="savannaensis">
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<emphasis id="B963EA9FFFBFC812D52CFB61FD8B57D0" bold="true" box="[261,516,1167,1189]" italics="true" pageId="5" pageNumber="25">Phyllosticta savannaensis</emphasis>
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</taxonomicName>
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</emphasis>
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(IFRD 9476, holotype). a. Microfungi on leaf litter. b–c. Ascomata on leaf surface. d–e. Vertical section of ascomata. f–i. Asci (i in cotton blue). j–n. Ascospores (m, n in cotton blue). Scale bars: b = 1000 μm, c= 500 μm, d = 100 μm, e, g–i = 30 μm, f = 50 μm, j–n = 10 μm.
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</paragraph>
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</caption>
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<subSubSection id="C30D6506FFBFC812D494FAF2FF74560B" pageId="5" pageNumber="25" type="materials_examined">
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<paragraph id="8BA8368DFFBFC812D494FAF2FF74560B" blockId="5.[136,1452,1308,1839]" pageId="5" pageNumber="25">
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<emphasis id="B963EA9FFFBFC812D494FAF2FE355643" bold="true" box="[189,442,1308,1334]" pageId="5" pageNumber="25">Material examined—</emphasis>
|
||
<materialsCitation id="3B7F3CD0FFBFC812D593FAF2FF74560B" collectingDate="2021-12" collectionCode="IFRD" collectorName="H. X. Wu & J. C. Li & X. H. Li" country="China" county="Yuanjiang County" elevation="579" latitude="23.467222" location="Yuanjiang National Nature Reserve" longLatPrecision="21" longitude="102.16778" pageId="5" pageNumber="25" specimenCode="IFRD 9476" specimenCount="1" stateProvince="Yunnan Province" typeStatus="holotype">
|
||
<collectingCountry id="F300761DFFBFC812D593FAF2FD995643" box="[442,534,1308,1334]" name="China" pageId="5" pageNumber="25">CHINA</collectingCountry>
|
||
.
|
||
<collectingRegion id="49D3F86FFFBFC812D636FAF3FD6D5643" box="[543,738,1308,1335]" country="China" name="Yunnan" pageId="5" pageNumber="25">Yunnan Province</collectingRegion>
|
||
:
|
||
<collectingCounty id="62C94E01FFBFC812D6C3FAF2FC395643" box="[746,950,1308,1334]" pageId="5" pageNumber="25">Yuanjiang County</collectingCounty>
|
||
,
|
||
<location id="8EC86056FFBFC812D797FAF2FAC45642" LSID="urn:lsid:plazi:treatment:03BE879BFFB8C812D4A1F8DAFBCF545A:8EC86056FFBFC812D797FAF2FAC45642" box="[958,1355,1308,1335]" country="China" county="Yuanjiang County" latitude="23.467222" longLatPrecision="21" longitude="102.16778" name="Yuanjiang National Nature Reserve" pageId="5" pageNumber="25" stateProvince="Yunnan Province">Yuanjiang National Nature Reserve</location>
|
||
, on dead leaves,
|
||
<geoCoordinate id="EE23504AFFBFC812D4F4FAAEFEDA562F" box="[221,341,1344,1370]" degrees="23" direction="north" minutes="28" orientation="latitude" pageId="5" pageNumber="25" precision="15" seconds="2" value="23.467222">23°28'2"N</geoCoordinate>
|
||
,
|
||
<geoCoordinate id="EE23504AFFBFC812D54BFAAEFE6A562F" box="[354,485,1344,1370]" degrees="102" direction="east" minutes="10" orientation="longitude" pageId="5" pageNumber="25" precision="15" seconds="4" value="102.16778">102°10'4"E</geoCoordinate>
|
||
,
|
||
<quantity id="4CEF9B68FFBFC812D5D8FAAEFDB6562E" box="[497,569,1344,1371]" metricMagnitude="2" metricUnit="m" metricValue="5.79" pageId="5" pageNumber="25" unit="m" value="579.0">
|
||
<elevation id="003AD1BEFFBFC812D5D8FAAEFDB6562E" box="[497,569,1344,1371]" metricMagnitude="2" metricUnit="m" metricValue="5.79" pageId="5" pageNumber="25" unit="m" value="579.0">579 m</elevation>
|
||
</quantity>
|
||
elev.,
|
||
<date id="FFA9104DFFBFC812D6ADFAAEFCB9562F" box="[644,822,1344,1370]" pageId="5" pageNumber="25" value="2021-12">
|
||
<collectingDate id="EFEDE9A5FFBFC812D6ADFAAEFCB9562F" box="[644,822,1344,1370]" pageId="5" pageNumber="25" value="2021-12">December 2021</collectingDate>
|
||
</date>
|
||
(dry season),
|
||
<collectorName id="26E2535BFFBFC812D7F1FAAFFBB0562E" box="[984,1087,1345,1371]" pageId="5" pageNumber="25">H.X. Wu</collectorName>
|
||
,
|
||
<collectorName id="26E2535BFFBFC812D065FAAEFA9B562F" box="[1100,1300,1344,1371]" pageId="5" pageNumber="25">J.C. Li & X.H. Li</collectorName>
|
||
(
|
||
<specimenCode id="DBB19EF6FFBFC812D10AFAAEFA28562F" box="[1315,1447,1344,1370]" pageId="5" pageNumber="25">IFRD 9476</specimenCode>
|
||
,
|
||
<typeStatus id="54AC882FFFBFC812D4A1FA8AFF60560B" box="[136,239,1380,1406]" pageId="5" pageNumber="25" type="holotype">holotype</typeStatus>
|
||
).
|
||
</materialsCitation>
|
||
</paragraph>
|
||
</subSubSection>
|
||
<subSubSection id="C30D6506FFBFC812D494FA66FBCF545A" pageId="5" pageNumber="25" type="discussion">
|
||
<paragraph id="8BA8368DFFBFC812D494FA66FBCF545A" blockId="5.[136,1452,1308,1839]" pageId="5" pageNumber="25">
|
||
<emphasis id="B963EA9FFFBFC812D494FA66FE8F56D7" bold="true" box="[189,256,1416,1442]" pageId="5" pageNumber="25">Notes</emphasis>
|
||
: According to phylogenetic tree analysis,
|
||
<taxonomicName id="4C174D0EFFBFC812D6CFFA67FC1656D7" box="[742,921,1417,1442]" class="Sordariomycetes" family="Diaporthaceae" genus="Phyllosticta" kingdom="Fungi" order="Diaporthales" pageId="5" pageNumber="25" phylum="Ascomycota" rank="species" species="savannaensis">
|
||
<emphasis id="B963EA9FFFBFC812D6CFFA67FC1656D7" box="[742,921,1417,1442]" italics="true" pageId="5" pageNumber="25">P. savannaensis</emphasis>
|
||
</taxonomicName>
|
||
is classified within the
|
||
<taxonomicName id="4C174D0EFFBFC812D087FA67FADE56D7" box="[1198,1361,1416,1442]" class="Sordariomycetes" family="Diaporthaceae" genus="Phyllosticta" kingdom="Fungi" order="Diaporthales" pageId="5" pageNumber="25" phylum="Ascomycota" rank="species" species="capitalensis">
|
||
<emphasis id="B963EA9FFFBFC812D087FA67FADE56D7" box="[1198,1361,1416,1442]" italics="true" pageId="5" pageNumber="25">P. capitalensis</emphasis>
|
||
</taxonomicName>
|
||
species complex (
|
||
<figureCitation id="132C2A08FFBFC812D4D2FA42FECF56B3" box="[251,320,1452,1478]" captionStart="FIGURE 1" captionStartId="3.[136,229,1824,1846]" captionTargetBox="[151,1435,422,1796]" captionTargetId="figure-150@3.[151,1435,422,1796]" captionTargetPageId="3" captionText="FIGURE 1. Phylogram of Phyllosticta resulting from a maximum likelihood analysis based on a combined matrix of ITS, LSU and TEF1 loci. The tree is artificially rooted to B. obtusa (CMW 8232). ML bootstrap values (left, ML-BS ≥ 50%) and Bayesian posterior probabilities (right, BYPP ≥ 0.9) are given at the nodes. New species of this study are indicated using red font and their associated support is bolded." figureDoi="http://doi.org/10.5281/zenodo.13216865" httpUri="https://zenodo.org/record/13216865/files/figure.png" pageId="5" pageNumber="25">Fig. 1</figureCitation>
|
||
), which contains 28 species (
|
||
<tableCitation id="C6950336FFBFC812D6B4FA42FD6156B3" box="[669,750,1452,1478]" captionStart="TABLE 1" captionStartId="6.[136,228,158,182]" captionTargetPageId="6" captionText="TABLE 1. A List of species, GenBank accession numbers of the sequences used for phylogenetic analysis. Sequences" pageId="5" pageNumber="25">Table1</tableCitation>
|
||
). The phylogenetic tree reveals that
|
||
<taxonomicName id="4C174D0EFFBFC812D0B0FA43FAC256B3" box="[1177,1357,1453,1478]" class="Sordariomycetes" family="Diaporthaceae" genus="Phyllosticta" kingdom="Fungi" order="Diaporthales" pageId="5" pageNumber="25" phylum="Ascomycota" rank="species" species="savannaensis">
|
||
<emphasis id="B963EA9FFFBFC812D0B0FA43FAC256B3" box="[1177,1357,1453,1478]" italics="true" pageId="5" pageNumber="25">P. savannaensis</emphasis>
|
||
</taxonomicName>
|
||
is most closely related to
|
||
<taxonomicName id="4C174D0EFFBFC812D563FA3FFE65569F" box="[330,490,1488,1514]" class="Sordariomycetes" family="Diaporthaceae" genus="Phyllosticta" kingdom="Fungi" order="Diaporthales" pageId="5" pageNumber="25" phylum="Ascomycota" rank="species" species="capitalensis">
|
||
<emphasis id="B963EA9FFFBFC812D563FA3FFE65569F" box="[330,490,1488,1514]" italics="true" pageId="5" pageNumber="25">P. capitalensis</emphasis>
|
||
</taxonomicName>
|
||
, but forms a single branch with strong support (100%/1.00, BS/
|
||
<collectionCode id="ED06AE48FFBFC812D083FA3EFB7A569F" box="[1194,1269,1488,1514]" pageId="5" pageNumber="25">BYPP</collectionCode>
|
||
). Morphological characters of
|
||
<taxonomicName id="4C174D0EFFBFC812D50DFA1BFE58557B" box="[292,471,1525,1550]" class="Sordariomycetes" family="Diaporthaceae" genus="Phyllosticta" kingdom="Fungi" order="Diaporthales" pageId="5" pageNumber="25" phylum="Ascomycota" rank="species" species="savannaensis">
|
||
<emphasis id="B963EA9FFFBFC812D50DFA1BFE58557B" box="[292,471,1525,1550]" italics="true" pageId="5" pageNumber="25">P. savannaensis</emphasis>
|
||
</taxonomicName>
|
||
and
|
||
<taxonomicName id="4C174D0EFFBFC812D63BFA1BFD39557B" box="[530,694,1524,1550]" class="Sordariomycetes" family="Diaporthaceae" genus="Phyllosticta" kingdom="Fungi" order="Diaporthales" pageId="5" pageNumber="25" phylum="Ascomycota" rank="species" species="capitalensis">
|
||
<emphasis id="B963EA9FFFBFC812D63BFA1BFD39557B" box="[530,694,1524,1550]" italics="true" pageId="5" pageNumber="25">P. capitalensis</emphasis>
|
||
</taxonomicName>
|
||
exhibit distinct differences, reinforcing the phylogenetic analysis. The size of ascospores and ratio of length to width differed between the two species (
|
||
<taxonomicName id="4C174D0EFFBFC812D06AF9F7FB6A5547" box="[1091,1253,1560,1586]" class="Sordariomycetes" family="Diaporthaceae" genus="Phyllosticta" kingdom="Fungi" order="Diaporthales" pageId="5" pageNumber="25" phylum="Ascomycota" rank="species" species="capitalensis">
|
||
<emphasis id="B963EA9FFFBFC812D06AF9F7FB6A5547" box="[1091,1253,1560,1586]" italics="true" pageId="5" pageNumber="25">P. capitalensis</emphasis>
|
||
</taxonomicName>
|
||
11–13 × 3–4 μm, length/width ratio 3.25:1 vs
|
||
<taxonomicName id="4C174D0EFFBFC812D5EEF9D3FDF75523" box="[455,632,1597,1622]" class="Sordariomycetes" family="Diaporthaceae" genus="Phyllosticta" kingdom="Fungi" order="Diaporthales" pageId="5" pageNumber="25" phylum="Ascomycota" rank="species" species="savannaensis">
|
||
<emphasis id="B963EA9FFFBFC812D5EEF9D3FDF75523" box="[455,632,1597,1622]" italics="true" pageId="5" pageNumber="25">P. savannaensis</emphasis>
|
||
</taxonomicName>
|
||
12–17 × 5–7 μm, length/width ratio 2.43:1).
|
||
<taxonomicName id="4C174D0EFFBFC812D050F9D3FAA55523" box="[1145,1322,1597,1622]" class="Sordariomycetes" family="Diaporthaceae" genus="Phyllosticta" kingdom="Fungi" order="Diaporthales" pageId="5" pageNumber="25" phylum="Ascomycota" rank="species" species="savannaensis">
|
||
<emphasis id="B963EA9FFFBFC812D050F9D3FAA55523" box="[1145,1322,1597,1622]" italics="true" pageId="5" pageNumber="25">P. savannaensis</emphasis>
|
||
</taxonomicName>
|
||
ascospores have appendages at both ends, with the apical appendage appearing as a hat and the bottom appendage as a flame, whereas
|
||
<taxonomicName id="4C174D0EFFBFC812D4C3F96BFE0455EB" box="[234,395,1668,1694]" class="Sordariomycetes" family="Diaporthaceae" genus="Phyllosticta" kingdom="Fungi" order="Diaporthales" pageId="5" pageNumber="25" phylum="Ascomycota" rank="species" species="capitalensis">
|
||
<emphasis id="B963EA9FFFBFC812D4C3F96BFE0455EB" box="[234,395,1668,1694]" italics="true" pageId="5" pageNumber="25">P. capitalensis</emphasis>
|
||
</taxonomicName>
|
||
ascospores are with or without a cap-shaped appendage at the end (
|
||
<tableCitation id="C6950336FFBFC812D054F96AFB5F55EB" box="[1149,1232,1668,1694]" captionStart="TABLE 2" captionStartId="10.[136,224,159,183]" captionTargetPageId="10" captionText="TABLE 2. Morphological characteristics comparison of Phyllosticta species related to this study. L to W = length/width ratio" pageId="5" pageNumber="25">Table2</tableCitation>
|
||
). In addition,
|
||
<collectionCode id="ED06AE48FFBFC812D141F96AFA2455EB" box="[1384,1451,1668,1694]" pageId="5" pageNumber="25">NCBI</collectionCode>
|
||
blast results of the
|
||
<collectionCode id="ED06AE48FFBFC812D548F946FE0455B7" box="[353,395,1704,1730]" pageId="5" pageNumber="25">ITS</collectionCode>
|
||
sequences of
|
||
<taxonomicName id="4C174D0EFFBFC812D607F947FD1055B7" box="[558,671,1704,1730]" class="Sordariomycetes" family="Diaporthaceae" genus="Phyllosticta" kingdom="Fungi" order="Diaporthales" pageId="5" pageNumber="25" phylum="Ascomycota" rank="species" species="ovalina">
|
||
<emphasis id="B963EA9FFFBFC812D607F947FD1055B7" box="[558,671,1704,1730]" italics="true" pageId="5" pageNumber="25">P. ovalina</emphasis>
|
||
</taxonomicName>
|
||
(
|
||
<typeStatus id="54AC882FFFBFC812D686F946FC9B55B7" box="[687,788,1704,1730]" pageId="5" pageNumber="25" type="holotype">holotype</typeStatus>
|
||
) also give support to our findings. The
|
||
<collectionCode id="ED06AE48FFBFC812D0F3F946FA9255B7" box="[1242,1309,1704,1730]" pageId="5" pageNumber="25">NCBI</collectionCode>
|
||
blast results of
|
||
<taxonomicName id="4C174D0EFFBFC812D480F923FED25593" box="[169,349,1741,1766]" class="Sordariomycetes" family="Diaporthaceae" genus="Phyllosticta" kingdom="Fungi" order="Diaporthales" pageId="5" pageNumber="25" phylum="Ascomycota" rank="species" species="savannaensis">
|
||
<emphasis id="B963EA9FFFBFC812D480F923FED25593" box="[169,349,1741,1766]" italics="true" pageId="5" pageNumber="25">P. savannaensis</emphasis>
|
||
</taxonomicName>
|
||
(
|
||
<typeStatus id="54AC882FFFBFC812D546F922FE5B5593" box="[367,468,1740,1766]" pageId="5" pageNumber="25" type="holotype">holotype</typeStatus>
|
||
) are as follows:
|
||
<taxonomicName id="4C174D0EFFBFC812D6B3F923FCC25593" box="[666,845,1741,1766]" class="Sordariomycetes" family="Diaporthaceae" genus="Phyllosticta" kingdom="Fungi" order="Diaporthales" pageId="5" pageNumber="25" phylum="Ascomycota" rank="species" species="savannaensis">
|
||
<emphasis id="B963EA9FFFBFC812D6B3F923FCC25593" box="[666,845,1741,1766]" italics="true" pageId="5" pageNumber="25">P. savannaensis</emphasis>
|
||
</taxonomicName>
|
||
and
|
||
<taxonomicName id="4C174D0EFFBFC812D7A3F923FBA05593" box="[906,1071,1740,1766]" class="Sordariomycetes" family="Diaporthaceae" genus="Phyllosticta" kingdom="Fungi" order="Diaporthales" pageId="5" pageNumber="25" phylum="Ascomycota" rank="species" species="capitalensis">
|
||
<emphasis id="B963EA9FFFBFC812D7A3F923FBA05593" box="[906,1071,1740,1766]" italics="true" pageId="5" pageNumber="25">P. capitalensis</emphasis>
|
||
</taxonomicName>
|
||
,
|
||
<taxonomicName id="4C174D0EFFBFC812D069F923FB4D5593" box="[1088,1218,1740,1766]" class="Sordariomycetes" family="Diaporthaceae" genus="Phyllosticta" kingdom="Fungi" order="Diaporthales" pageId="5" pageNumber="25" phylum="Ascomycota" rank="species" species="fallopiae">
|
||
<emphasis id="B963EA9FFFBFC812D069F923FB4D5593" box="[1088,1218,1740,1766]" italics="true" pageId="5" pageNumber="25">P. fallopiae</emphasis>
|
||
</taxonomicName>
|
||
,
|
||
<taxonomicName id="4C174D0EFFBFC812D0FAF923FADC5593" box="[1235,1363,1740,1766]" class="Sordariomycetes" family="Diaporthaceae" genus="Phyllosticta" kingdom="Fungi" order="Diaporthales" pageId="5" pageNumber="25" phylum="Ascomycota" rank="species" species="elongata">
|
||
<emphasis id="B963EA9FFFBFC812D0FAF923FADC5593" box="[1235,1363,1740,1766]" italics="true" pageId="5" pageNumber="25">P. elongata</emphasis>
|
||
</taxonomicName>
|
||
exhibit only 92.04% sequence identity (query cover = 99%) (
|
||
<tableCitation id="C6950336FFBFC812D6CEF91EFCB0547F" box="[743,831,1776,1802]" captionStart="TABLE 3" captionStartId="11.[136,216,1024,1045]" captionTargetPageId="11" captionText="TABLE 3. GenBank BLAST search results of Phyllosticta that clustered with P. savannaensis and P. ovalina." pageId="5" pageNumber="25">Table 3</tableCitation>
|
||
). Based on morphological characters and phylogenetic analysis,
|
||
<taxonomicName id="4C174D0EFFBFC812D4D9F8FBFE2E545B" box="[240,417,1813,1838]" class="Sordariomycetes" family="Diaporthaceae" genus="Phyllosticta" kingdom="Fungi" order="Diaporthales" pageId="5" pageNumber="25" phylum="Ascomycota" rank="species" species="savannaensis">
|
||
<emphasis id="B963EA9FFFBFC812D4D9F8FBFE2E545B" box="[240,417,1813,1838]" italics="true" pageId="5" pageNumber="25">P. savannaensis</emphasis>
|
||
</taxonomicName>
|
||
is identified as a new species within the genus
|
||
<taxonomicName id="4C174D0EFFBFC812D79AF8FAFBB6545B" box="[947,1081,1812,1838]" class="Sordariomycetes" family="Diaporthaceae" genus="Phyllosticta" kingdom="Fungi" order="Diaporthales" pageId="5" pageNumber="25" phylum="Ascomycota" rank="genus">
|
||
<emphasis id="B963EA9FFFBFC812D79AF8FAFBB6545B" box="[947,1081,1812,1838]" italics="true" pageId="5" pageNumber="25">Phyllosticta</emphasis>
|
||
</taxonomicName>
|
||
.
|
||
</paragraph>
|
||
</subSubSection>
|
||
</treatment>
|
||
</document> |