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<mods:title id="A9CF57934494EB9A1A99C90C5FFC865B">Two new green algae, Rhizoclonium fractum sp. nov. and R. umbraticum sp. nov., from tropical and subtropical brackish waters of Japan</mods:title>
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<mods:affiliation id="11D19967787AC18D1EA3D4A6CB506337">Department of Biology, Faculty of Science, Toho University, 2 - 2 - 1 Miyama, Funabashi, Chiba 274 - 8510, Japan &amp; Graduate School of Frontier Science, Department of Integrated Biosciences, University of Tokyo, 5 - 1 - 5 Kashiwanoha, Kashiwa, Chiba 277 - 8562, Japan</mods:affiliation>
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<taxonomicName id="4C6E4D59FF86FFAFFF7EFC8DFD14FC00" authority="(Zeller) P. C. Silva" authorityName="P. C. Silva" baseAuthorityName="Zeller" box="[136,687,884,911]" class="Ulvophyceae" family="Cladophoraceae" genus="Rhizoclonium" kingdom="Plantae" order="Cladophorales" pageId="15" pageNumber="246" phylum="Chlorophyta" rank="species" species="minutissimum">
<emphasis id="B91AEAC8FF86FFAFFF7EFC8DFE6DFC01" bold="true" box="[136,470,884,911]" italics="true" pageId="15" pageNumber="246">Rhizoclonium minutissimum</emphasis>
(Zeller) P. C. Silva
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(
<figureCitation id="13552A5FFF86FFAFFD4BFC8FFCB6FC00" box="[701,781,886,911]" captionStart="FIGURE 7" captionStartId="11.[136,229,1547,1568]" captionTargetBox="[158,1433,394,1517]" captionTargetId="figure-123@11.[151,1436,389,1521]" captionTargetPageId="11" captionText="FIGURE 7. Field-collected specimens of Cladophoraceae sp. 2 (Rhizoclonium minutissimum). (A) Field-collected specimens. (B, C) Morphology of field-collected specimens. (D, E) Filamentous thalli with non-septate rhizoids. (F) Tip of filamentous thalli. (GI) Spindle- and band-shaped chloroplasts. Arrowhead points to a pyrenoid. (J) Autofluorescence of chloroplasts. (K) Cells stained with DAPI, showing nuclei. (L) Cells stained with Lugols iodine, showing pyrenoids. (M, N) Pyrenoids observed by transmission electron microscopy. Pyrenoids have starch plates. (M) Three or two thylakoid membranes traversing a pyrenoid. (33) One thylakoid membrane. Scale bar = 5 mm (A); Scale bars = 40 μm (B, C); Scale bars = 20 μm (DG, K, L); Scale bars = 10 μm (HJ); Scale bars = 0.5 μm (M); Scale bars = 1 μm (N)." figureDoi="http://doi.org/10.5281/zenodo.13658128" httpUri="https://zenodo.org/record/13658128/files/figure.png" pageId="15" pageNumber="246">Figs. 7</figureCitation>
,
<figureCitation id="13552A5FFF86FFAFFCEFFC8FFC9CFC00" box="[793,807,886,911]" captionStart="FIGURE 8" captionStartId="12.[136,229,1279,1300]" captionTargetBox="[155,1433,194,1250]" captionTargetId="figure-16@12.[151,1436,190,1254]" captionTargetPageId="12" captionText="FIGURE 8. Cultured material of Cladophoraceae sp. 2 (Rhizoclonium minutissimum). (A, B) Morphology of filamentous thalli. (C) Filamentous thalli with rhizoid-like structure. (D, E) Spindle- and band-shaped chloroplasts with pyrenoids (arrowheads). (F) Autofluorescence of chloroplasts. (G) Cells stained with DAPI, showing nuclei. (H) Cells stained with Lugols iodine, showing the pyrenoids. (I) Mature sporangia with spores and vegetative cells. Arrowhead points to a mature sporangium. (J) Detail of a hapteroid attachment cell. Scale bar = 20 μm (AC, GI); Scale bars = 10 μm (DF, K)." figureDoi="http://doi.org/10.5281/zenodo.13658132" httpUri="https://zenodo.org/record/13658132/files/figure.png" pageId="15" pageNumber="246">8</figureCitation>
)
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<paragraph id="8BD136DAFF86FFAFFF7EFC63FB60FC58" blockId="15.[136,1452,922,1019]" pageId="15" pageNumber="246">
Basionym:
<taxonomicName id="4C6E4D59FF86FFAFFEF1FC63FD1FFC3C" authority="Zeller 1873" authorityName="Zeller" authorityYear="1873" box="[263,676,922,947]" class="Ulvophyceae" family="Cladophoraceae" genus="Cladophora" kingdom="Plantae" order="Cladophorales" pageId="15" pageNumber="246" phylum="Chlorophyta" rank="species" species="minutissima">
<emphasis id="B91AEAC8FF86FFAFFEF1FC63FDA0FC3C" box="[263,539,922,947]" italics="true" pageId="15" pageNumber="246">Cladophora minutissima</emphasis>
<bibRefCitation id="EFFF4B2BFF86FFAFFDD7FC63FD1FFC3C" author="Zeller, G." box="[545,676,922,947]" pageId="15" pageNumber="246" pagination="175 - 193" refId="ref11739" refString="Zeller, G. (1873) Algae collected by Mr. S. Kurz in Arracan and British Burma, determined and systematically arranged by Dr. G. Zeller. Journal of the Asiatic Society of Bengal 42: 175 - 193." type="journal article" year="1873">Zeller 1873</bibRefCitation>
</taxonomicName>
, Algae collected by S. Kurz in Arracan and British
<collectingCountry id="F379764AFF86FFAFFB23FC63FA9FFC3C" box="[1237,1316,922,947]" name="Myanmar" pageId="15" pageNumber="246">Burma</collectingCountry>
, determined and systematically arranged by G. Zeller.
<emphasis id="B91AEAC8FF86FFAFFDAAFC47FBA3FC58" box="[604,1048,958,983]" italics="true" pageId="15" pageNumber="246">Journal of the Asiatic Society of Bengal</emphasis>
42 (2): 175193.
</paragraph>
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<paragraph id="8BD136DAFF86FFAFFF4BFC1BFC0DFC74" blockId="15.[136,1452,922,1019]" box="[189,950,994,1019]" pageId="15" pageNumber="246">
<typeStatus id="54D58878FF86FFAFFF4BFC1BFF4EFC74" box="[189,245,994,1019]" pageId="15" pageNumber="246">Type</typeStatus>
locality: Elephant Point,
<collectingRegion id="49AAF838FF86FFAFFDE5FC1BFDF5FC74" box="[531,590,994,1019]" country="Myanmar" name="Bago" pageId="15" pageNumber="246">Pegu</collectingRegion>
,
<collectingCountry id="F379764AFF86FFAFFDAFFC1BFD1EFC74" box="[601,677,994,1019]" name="Myanmar" pageId="15" pageNumber="246">Burma</collectingCountry>
(
<bibRefCitation id="EFFF4B2BFF86FFAFFD42FC1BFC12FC74" author="Silva, P. C. &amp; Basson, P. W. &amp; Moe, R. L." box="[692,937,994,1019]" pageId="15" pageNumber="246" pagination="1 - 1259" refId="ref11217" refString="Silva, P. C., Basson, P. W. &amp; Moe, R. L. (1996) Catalogue of the benthic marine algae of the Indian Ocean. University of California Publications in Botany 79: 1 - 1259." type="journal article" year="1996">
Silva
<emphasis id="B91AEAC8FF86FFAFFD03FC1BFC95FC74" box="[757,814,994,1019]" italics="true" pageId="15" pageNumber="246">et al.</emphasis>
1996: 785
</bibRefCitation>
).
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