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<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.43.25081" ID-GBIF-Dataset="d9c55c7b-4a9f-4045-bd20-09dd6902b3fe" ID-PMC="PMC6290043" ID-Pensoft-Pub="1314-4049--23" ID-PubMed="30568535" ModsDocAuthor="" ModsDocDate="2018" ModsDocID="1314-4049--23" ModsDocOrigin="MycoKeys " ModsDocTitle="Endophytic Colletotrichum species from Dendrobium spp. in China and Northern Thailand" checkinTime="1553125590011" checkinUser="pensoft" docAuthor="Ma, Xiaoya, Nontachaiyapoom, Sureeporn, Jayawardena, Ruvishika S., yde, Kevin D., Gentekaki, Eleni, Zhou, Sixuan, Qian, Yixin, Wen, Tingchi &amp; Kang, Jichuan" docDate="2018" docId="6A13E6B70C777D56161F93FB442F4D00" docLanguage="en" docName="MycoKeys 43: 23-57" docOrigin="MycoKeys 43" docSource="http://dx.doi.org/10.3897/mycokeys.43.25081" docTitle="Colletotrichum cariniferi X. Y. Ma, K. D. Hyde &amp; Jayawardena, sp.nov." docType="treatment" docVersion="4" lastPageNumber="29" masterDocId="FF97FFBBFFBCFFCF2B24FFA89B70FFD8" masterDocTitle="Endophytic Colletotrichum species from Dendrobium spp. in China and Northern Thailand" masterLastPageNumber="57" masterPageNumber="23" pageNumber="29" updateTime="1668136111028" updateUser="ExternalLinkService">
<mods:mods xmlns:mods="http://www.loc.gov/mods/v3">
<mods:titleInfo>
<mods:title>Endophytic Colletotrichum species from Dendrobium spp. in China and Northern Thailand</mods:title>
</mods:titleInfo>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Ma, Xiaoya</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Nontachaiyapoom, Sureeporn</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Jayawardena, Ruvishika S.</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>yde, Kevin D.</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Gentekaki, Eleni</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Zhou, Sixuan</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Qian, Yixin</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Wen, Tingchi</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Kang, Jichuan</mods:namePart>
</mods:name>
<mods:typeOfResource>text</mods:typeOfResource>
<mods:relatedItem type="host">
<mods:titleInfo>
<mods:title>MycoKeys</mods:title>
</mods:titleInfo>
<mods:part>
<mods:date>2018</mods:date>
<mods:detail type="volume">
<mods:number>43</mods:number>
</mods:detail>
<mods:extent unit="page">
<mods:start>23</mods:start>
<mods:end>57</mods:end>
</mods:extent>
</mods:part>
</mods:relatedItem>
<mods:location>
<mods:url>http://dx.doi.org/10.3897/mycokeys.43.25081</mods:url>
</mods:location>
<mods:classification>journal article</mods:classification>
<mods:identifier type="DOI">http://dx.doi.org/10.3897/mycokeys.43.25081</mods:identifier>
<mods:identifier type="Pensoft-Pub">1314-4049--23</mods:identifier>
</mods:mods>
<treatment ID-GBIF-Taxon="154473873" LSID="urn:lsid:plazi:treatment:6A13E6B70C777D56161F93FB442F4D00" httpUri="http://treatment.plazi.org/id/6A13E6B70C777D56161F93FB442F4D00" lastPageId="7" lastPageNumber="29" pageId="6" pageNumber="29">
<subSubSection pageId="6" pageNumber="29" type="nomenclature">
<paragraph pageId="6" pageNumber="29">
<taxonomicName authority="X. Y. Ma, K. D. Hyde &amp; Jayawardena" class="Sordariomycetes" family="Glomerellaceae" genus="Colletotrichum" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Colletotrichum cariniferi" order="Glomerellales" pageId="6" pageNumber="29" phylum="Ascomycota" rank="species" species="cariniferi">Colletotrichum cariniferi X.Y. Ma, K.D. Hyde &amp; Jayawardena</taxonomicName>
<taxonomicNameLabel pageId="6" pageNumber="29">sp.nov.</taxonomicNameLabel>
</paragraph>
</subSubSection>
<subSubSection pageId="6" pageNumber="29" type="etymology">
<paragraph pageId="6" pageNumber="29">Etymology.</paragraph>
<paragraph pageId="6" pageNumber="29">In reference to the host epithet cariniferum.</paragraph>
</subSubSection>
<subSubSection pageId="6" pageNumber="29" type="holotype">
<paragraph pageId="6" pageNumber="29">Holotype.</paragraph>
<paragraph pageId="6" pageNumber="29">MFLC 17-1199 (ex-holotype culture: MFLUCC 14-0100).</paragraph>
</subSubSection>
<subSubSection pageId="6" pageNumber="29" type="description">
<paragraph pageId="6" pageNumber="29">Description.</paragraph>
<paragraph pageId="6" pageNumber="29">Sexual morph not observed.</paragraph>
<paragraph pageId="6" pageNumber="29">
Asexual morph on CMA. Vegetative hyphae 3.5-8.2
<normalizedToken originalValue="µm">µm</normalizedToken>
diam (N=20), hyaline to brown, smooth-walled, septate, branched. Appressoria (9.7-) 10.4-17 (-20.5)
<normalizedToken originalValue="×">x</normalizedToken>
(6.5-) 7.1-11.3 (-13.6)
<normalizedToken originalValue="µm">µm</normalizedToken>
(N=6), globose to sub-globose, light brown. Conidiomata 50
<normalizedToken originalValue="×">x</normalizedToken>
50
<normalizedToken originalValue="µm">µm</normalizedToken>
(N=10), developing with mycelia, globose to irregular, milk orange to orange, in mass brown. Conidiophores (37.5-) 42.3-65 (-71.6)
<normalizedToken originalValue="×">x</normalizedToken>
(3.1-) 3.8-5.9 (-6)
<normalizedToken originalValue="µm">µm</normalizedToken>
(N=6), smooth-walled, unbranched, hyaline. The part connected with conidia of conidiogenous cell inflated and some with large guttules. Conidia (24.1-) 26.8-33.0 (-36.1)
<normalizedToken originalValue="×">x</normalizedToken>
(7.9-) 8.3-9.6 (-10.2)
<normalizedToken originalValue="µm">µm</normalizedToken>
(N=30), L/W = 3.4, ellipsoidal to cylindrical, with one end inflated when immature state, both ends rounded when mature, with 2 to 3 guttules, hyaline.
</paragraph>
<paragraph pageId="6" pageNumber="29">Cultures on CMA flat with entire margin. Growth rate: 0.23cm/day, with 50-days for sporulation. Cottony, pale cinnamon to light brown, scattered pale mycelia in spots around the middle inoculum clump, sometimes covered short, floccose-felty, white, aerial mycelium, reverse buff brown.</paragraph>
<caption pageId="6" pageNumber="29">
<paragraph pageId="6" pageNumber="29">
Figure 2.
<taxonomicName class="Dothideomycetes" family="Botryosphaeriaceae" genus="Colletotrichum" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Colletotrichum cariniferi" order="Botryosphaeriales" pageId="6" pageNumber="29" phylum="Ascomycota" rank="species" species="cariniferi">Colletotrichum cariniferi</taxonomicName>
(holotype). A Colony B Conidiomata C,
<normalizedToken originalValue="IJ">I-J</normalizedToken>
Appressoria
<normalizedToken originalValue="DH">D-H</normalizedToken>
Conidiophores
<normalizedToken originalValue="KM">K-M</normalizedToken>
Conidia. Scale bars: 100
<normalizedToken originalValue="µm">µm</normalizedToken>
(B), 5
<normalizedToken originalValue="µm">µm</normalizedToken>
(
<normalizedToken originalValue="CD">C-D</normalizedToken>
), 10
<normalizedToken originalValue="µm">µm</normalizedToken>
(
<normalizedToken originalValue="EH">E-H</normalizedToken>
), 5
<normalizedToken originalValue="µm">µm</normalizedToken>
(
<normalizedToken originalValue="IM">I-M</normalizedToken>
).
</paragraph>
</caption>
</subSubSection>
<subSubSection pageId="6" pageNumber="29" type="materials_examined">
<paragraph pageId="6" pageNumber="29">Material examined.</paragraph>
<paragraph pageId="6" pageNumber="29">
Thailand, Chiang Rai, Wat Phra That Doi Tung (Temple of Doi Tung Pagodas), the host
<taxonomicName class="Liliopsida" family="Orchidaceae" genus="Dendrobium" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Dendrobium cariniferum" order="Asparagales" pageId="6" pageNumber="29" phylum="Tracheophyta" rank="species" species="cariniferum">Dendrobium cariniferum</taxonomicName>
was sampled on 19 December 2013, Collector: Sureeporn Nontachaiyapoom, Natdanai Aewsakul, Xiaoya Ma.
</paragraph>
</subSubSection>
<subSubSection lastPageId="7" lastPageNumber="30" pageId="6" pageNumber="29" type="notes">
<paragraph pageId="6" pageNumber="29">Notes.</paragraph>
<paragraph lastPageId="7" lastPageNumber="30" pageId="6" pageNumber="29">
<taxonomicName class="Dothideomycetes" family="Botryosphaeriaceae" genus="Colletotrichum" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Colletotrichum cariniferi" order="Botryosphaeriales" pageId="6" pageNumber="29" phylum="Ascomycota" rank="species" species="cariniferi">Colletotrichum cariniferi</taxonomicName>
(MFLUCC 14-0100) clusters in a separate branch with a good support (88ML/1.00BI/59MP). The species is most phylogenetically close to
<taxonomicName class="Dothideomycetes" family="Botryosphaeriaceae" genus="Colletotrichum" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Colletotrichum excelsum-altitudum" order="Botryosphaeriales" pageId="6" pageNumber="29" phylum="Ascomycota" rank="species" species="excelsum-altitudum">Colletotrichum excelsum-altitudum</taxonomicName>
and
<taxonomicName lsidName="C. tropicicola" pageId="6" pageNumber="29" rank="species" species="tropicicola">C. tropicicola</taxonomicName>
, but they are morphologically different.
<taxonomicName lsidName="C. cariniferi" pageId="6" pageNumber="29" rank="species" species="cariniferi">C. cariniferi</taxonomicName>
holds 77 and 91 different base pairs compared with
<taxonomicName lsidName="C. tropicicola" pageId="6" pageNumber="29" rank="species" species="tropicicola">C. tropicicola</taxonomicName>
and
<taxonomicName lsidName="C. excelsum-altitudum" pageId="6" pageNumber="29" rank="species" species="excelsum-altitudum">C. excelsum-altitudum</taxonomicName>
respectively.
<taxonomicName class="Dothideomycetes" family="Botryosphaeriaceae" genus="Colletotrichum" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Colletotrichum cariniferi" order="Botryosphaeriales" pageId="6" pageNumber="29" phylum="Ascomycota" rank="species" species="cariniferi">Colletotrichum cariniferi</taxonomicName>
has much larger conidia than that of closely related strains in the tree (conidia (24.1-) 26.8-33 (-36.1)
<normalizedToken originalValue="×">x</normalizedToken>
(7.9-) 8.3-9.6 (-10.2)
<normalizedToken originalValue="µm">µm</normalizedToken>
(N=30), L/W = 3.4 vs. conidia of
<taxonomicName lsidName="C. tropicicola" pageId="6" pageNumber="29" rank="species" species="tropicicola">C. tropicicola</taxonomicName>
13
<normalizedToken originalValue="16.5×5">-16.5x5-</normalizedToken>
7
<normalizedToken originalValue="μm">μm</normalizedToken>
<pageBreakToken pageId="7" pageNumber="30" start="start">and</pageBreakToken>
<taxonomicName lsidName="C. excelsum-altitudum" pageId="7" pageNumber="30" rank="species" species="excelsum-altitudum">C. excelsum-altitudum</taxonomicName>
14.8
<normalizedToken originalValue="±">+/-</normalizedToken>
0.8
<normalizedToken originalValue="×">x</normalizedToken>
5.8
<normalizedToken originalValue="±">+/-</normalizedToken>
0.4
<normalizedToken originalValue="μm">μm</normalizedToken>
) (Noireung 2012;
<bibRefCitation author="Tao, G" journalOrPublisher="Fungal Diversity" pageId="26" pageNumber="49" pagination="139 - 164" title="Endophytic Colletotrichum species from Bletillaochracea (Orchidaceae) with descriptions of seven new species." url="https://doi.org/10.1007/s13225-013-0254-5" volume="61" year="2013">Tao et al. 2013</bibRefCitation>
). Blastn searches with sequence of MFLUCC 14-0100 resulted in 100% matches with ITS sequence of endophytic
<taxonomicName class="Dothideomycetes" family="Botryosphaeriaceae" genus="Colletotrichum" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Colletotrichum" order="Botryosphaeriales" pageId="7" pageNumber="30" phylum="Ascomycota" rank="genus">Colletotrichum</taxonomicName>
sp. strain S4 isolated from
<taxonomicName genus="Dendrobuim" lsidName="Dendrobuim nobile" pageId="7" pageNumber="30" rank="species" species="nobile">Dendrobuim nobile</taxonomicName>
in China (GenBank FJ042517,
<bibRefCitation author="Yuan, Z" journalOrPublisher="World Journal of Microbiology and Biotechnology" pageId="26" pageNumber="49" pagination=": 295" title="Diverse non-mycorrhizal fungal endophytes inhabiting an epiphytic, medicinal orchid (Dendrobiumnobile): estimation and characterization." url="https://doi.org/10.1007/s11274-008-9893-1" volume="25" year="2009">Yuan et al. 2009</bibRefCitation>
) and 96% identity with TUB2 sequences of
<taxonomicName lsidName="C. arxii" pageId="7" pageNumber="30" rank="species" species="arxii">C. arxii</taxonomicName>
strain CBS 169.59 isolated from
<taxonomicName class="Liliopsida" family="Orchidaceae" genus="Oncidium" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Oncidium excavatum" order="Asparagales" pageId="7" pageNumber="30" phylum="Tracheophyta" rank="species" species="excavatum">Oncidium excavatum</taxonomicName>
(GenBank KF687868,
<bibRefCitation author="Liu, F" journalOrPublisher="Persoonia - Molecular Phylogeny and Evolution of Fungi" pageId="24" pageNumber="47" pagination="83 - 97" title="The Colletotrichumgigasporum species complex." url="https://doi.org/10.3767/003158514X684447" volume="33" year="2014">Liu et al. 2014</bibRefCitation>
) in Netherlands and another
<taxonomicName lsidName="C. arxii" pageId="7" pageNumber="30" rank="species" species="arxii">C. arxii</taxonomicName>
strain CBS 132511 isolated from
<taxonomicName genus="Paphlopedilum" lsidName="Paphlopedilum" pageId="7" pageNumber="30" rank="genus">Paphlopedilum</taxonomicName>
sp. in Germany (GenBank KF687881,
<bibRefCitation author="Liu, F" journalOrPublisher="Persoonia - Molecular Phylogeny and Evolution of Fungi" pageId="24" pageNumber="47" pagination="83 - 97" title="The Colletotrichumgigasporum species complex." url="https://doi.org/10.3767/003158514X684447" volume="33" year="2014">Liu et al. 2014</bibRefCitation>
) respectively.
<taxonomicName class="Dothideomycetes" family="Botryosphaeriaceae" genus="Colletotrichum" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Colletotrichum cariniferi" order="Botryosphaeriales" pageId="7" pageNumber="30" phylum="Ascomycota" rank="species" species="cariniferi">Colletotrichum cariniferi</taxonomicName>
from stems of
<taxonomicName lsidName="D. cariniferum" pageId="7" pageNumber="30" rank="species" species="cariniferum">D. cariniferum</taxonomicName>
is introduced as a new species.
</paragraph>
</subSubSection>
</treatment>
</document>