184 lines
31 KiB
XML
184 lines
31 KiB
XML
<document id="5DE7B897BB73CE6D0FB1237683152346" ID-DOI="10.1080/00222933.2017.1319520" ID-ISSN="1464-5262" ID-Zenodo-Dep="5181143" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.tables_requiresApprovalFor="existingObjects,plazi" IM.taxonomicNames_approvedBy="felipe" checkinTime="1628687010221" checkinUser="felipe" docAuthor="Jang, Seok Won, Vďačný, Peter, Shazib, Shahed Uddin Ahmed & Shin, Mann Kyoon" docDate="2017" docId="03B0879FFFAEFFAD660DFF0BFD7237B2" docLanguage="en" docName="JNatHist.51.17-18.939-974.pdf" docOrigin="Journal of Natural History 51 (17 - 18)" docSource="http://dx.doi.org/10.1080/00222933.2017.1319520" docStyle="DocumentStyle:485A7191CD41D6261F41521A3380DC73.9:JNatHist.2017-.journal_article.1cover" docStyleId="485A7191CD41D6261F41521A3380DC73" docStyleName="JNatHist.2017-.journal_article.1cover" docStyleVersion="9" docTitle="Spathidium polynucleatum Jang & Vďačný & Shazib & Shin 2017, comb. nov." docType="treatment" docVersion="3" lastPageNumber="959" masterDocId="FF89FFE7FFBCFFB8672FFF86FFA6375E" masterDocTitle="Linking morphology and molecules: integrative taxonomy of spathidiids (Protista: Ciliophora: Litostomatea) from Korea" masterLastPageNumber="974" masterPageNumber="939" pageNumber="956" updateTime="1699049680283" updateUser="plazi" zenodo-license-document="CLOSED">
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<mods:title id="3062A0BA416FC2B6FF76AB423B67FFD5">Linking morphology and molecules: integrative taxonomy of spathidiids (Protista: Ciliophora: Litostomatea) from Korea</mods:title>
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<mods:namePart id="661C7029D427762276BED36D73644660">Jang, Seok Won</mods:namePart>
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<mods:namePart id="1086EC2D410D21C3F9DEC02AA5CA3AD4">Vďačný, Peter</mods:namePart>
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<mods:namePart id="95C442327E414B48E8AB98B1049DF86D">Shazib, Shahed Uddin Ahmed</mods:namePart>
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<mods:namePart id="A3E79F89BE64E36118BCD28F0A87A8BE">Shin, Mann Kyoon</mods:namePart>
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<mods:title id="0439A3E9FDF8510D534E0A4D43D9B228">Journal of Natural History</mods:title>
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<mods:date id="D610F3FC46345D92253B2F5E603B6934">2017</mods:date>
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<mods:number id="DB803CF345B48DEC1035D50E1C7F0749">2017-05-15</mods:number>
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<mods:number id="66FDF48E1E2A5B47F648E8B7A7D000B7">51</mods:number>
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<mods:number id="D7506AB621E0D393EDBF0B3332F9176F">17 - 18</mods:number>
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<subSubSection id="C3036502FFAEFFAA660DFF0BFBA437F9" box="[290,1026,141,167]" pageId="18" pageNumber="956" type="nomenclature">
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<paragraph id="8BA63689FFAEFFAA660DFF0BFBA437F9" blockId="18.[290,1026,141,202]" box="[290,1026,141,167]" pageId="18" pageNumber="956">
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<heading id="D0EE81E5FFAEFFAA660DFF0BFBA437F9" box="[290,1026,141,167]" centered="true" fontSize="11" level="2" pageId="18" pageNumber="956" reason="5">
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<taxonomicName id="4C194D0AFFAEFFAA660DFF0BFCD237F9" authority="(Foissner et al., 2002)" authorityName="Jang & Vďačný & Shazib & Shin" authorityYear="2017" baseAuthorityName="Jang & Vďačný & Shazib & Shin" baseAuthorityYear="2002" box="[290,884,141,167]" class="Gymnostomatea" family="Spathidiidae" genus="Spathidium" kingdom="Chromista" order="Spathidiida" pageId="18" pageNumber="956" phylum="Ciliophora" rank="species" species="polynucleatum" status="comb. nov.">
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<emphasis id="B96DEA9BFFAEFFAA660DFF0BFDC837F9" bold="true" box="[290,622,141,167]" italics="true" pageId="18" pageNumber="956">Spathidium polynucleatum</emphasis>
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(
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<bibRefCitation id="EF884B78FFAEFFAA6552FF08FCCD37F9" author="Foissner W & Agatha S & Berger H" box="[637,875,142,167]" pageId="18" pageNumber="956" pagination="1 - 1459" refId="ref14095" refString="Foissner W, Agatha S, Berger H. 2002. Soil ciliates (Protozoa, Ciliophora) from Namibia (Southwest Africa) with emphasis on two contrasting environments, the Etosha region and the Namib Desert. Denisia. 5: 1 - 1459." type="journal article" year="2002">Foissner et al., 2002</bibRefCitation>
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)
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</taxonomicName>
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<taxonomicNameLabel id="A25E57E0FFAEFFAA6452FF08FBA437F9" box="[893,1026,142,167]" pageId="18" pageNumber="956" rank="species">comb. nov.</taxonomicNameLabel>
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</heading>
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</paragraph>
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</subSubSection>
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<subSubSection id="C3036502FFAEFFAA66EDFF37FCC73794" box="[450,865,177,202]" pageId="18" pageNumber="956" type="description">
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<paragraph id="8BA63689FFAEFFAA66EDFF37FCC73794" blockId="18.[290,1026,141,202]" box="[450,865,177,202]" pageId="18" pageNumber="956">
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(
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<figureCitation id="13222A0CFFAEFFAA66E5FF37FD913794" box="[458,567,177,202]" captionStart="Figure 8" captionStartId="19.[160,225,1556,1579]" captionTargetBox="[163,1153,142,1525]" captionTargetId="figure-1@19.[163,1153,142,1525]" captionTargetPageId="19" captionText="Figure 8. Spathidium polynucleatum from life (a, c, g) and after protargol impregnation (b, d–f). (a) Right side view of a representative individual; (b) developing cytoplasmic extrusomes, 4–8 µm long; (c) oral extrusomes, 8–10 µm long; (d, e) right and left side view of ciliary pattern and nuclear apparatus of a representative specimen; (f) dorsal view of ciliary pattern in anterior body portion; (g) cortical granulation. CG, cortical granules; CK, circumoral kinety; DB, dorsal brush; MA, macronuclear nodules/macronucleus; MI, micronuclei/micronucleus; OB, oral bulge; SC, somatic cilia; SK, somatic kinety/somatic kineties. Scale bars: a, d, e = 100 µm; f = 30 µm." figureDoi="http://doi.org/10.5281/zenodo.5181153" httpUri="https://zenodo.org/record/5181153/files/figure.png" pageId="18" pageNumber="956">Figures 8</figureCitation>
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(a–g) and 9(a–l);
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<tableCitation id="C69B0332FFAEFFAA642EFF37FCFF3794" box="[769,857,177,202]" captionStart="Table 2" captionStartId="7.[160,216,142,165]" captionText="Table 2. Morphometric data of Korean populations of Enchelys megaspinata sp. nov. (EM), Spathidium ascendens (SA), S. papilliferum (SPa, population 2), S. polynucleatum comb. nov. (SPo), S. rectitoratum (SR), and Apobryophyllum schmidingeri (AS)." pageId="18" pageNumber="956">Table 2</tableCitation>
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)
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</paragraph>
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</subSubSection>
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<subSubSection id="C3036502FFAEFFAA678FFF63FC5737A0" box="[160,1009,229,254]" pageId="18" pageNumber="956" type="reference_group">
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<paragraph id="8BA63689FFAEFFAA678FFF63FC5737A0" blockId="18.[160,1009,229,254]" box="[160,1009,229,254]" pageId="18" pageNumber="956">
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<treatmentCitationGroup id="AB0911A7FFAEFFAA678FFF63FC5737A0" box="[160,1009,229,254]" pageId="18" pageNumber="956">
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<treatmentCitation id="0AB81098FFAEFFAA678FFF63FCAC37A0" author="Foissner W & Agatha S & Berger H" box="[160,778,229,254]" page="312" pageId="18" pageNumber="956" year="2002">
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<taxonomicName id="4C194D0AFFAEFFAA678FFF63FCAC37A0" ID-CoL="6FVS3" authority="Foissner et al., 2002: 312" authorityName="Foissner" authorityPageNumber="312" authorityYear="2002" box="[160,778,229,254]" class="Gymnostomatea" family="Spathidiidae" genus="Epispathidium" kingdom="Chromista" order="Spathidiida" pageId="18" pageNumber="956" phylum="Ciliophora" rank="species" species="polynucleatum">
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<emphasis id="B96DEA9BFFAEFFAA678FFF63FE4337A0" box="[160,485,229,254]" italics="true" pageId="18" pageNumber="956">Epispathidium polynucleatum</emphasis>
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<bibRefCitation id="EF884B78FFAEFFAA66C1FF60FCAC37A0" author="Foissner W & Agatha S & Berger H" box="[494,778,230,254]" pageId="18" pageNumber="956" pagination="1 - 1459" refId="ref14095" refString="Foissner W, Agatha S, Berger H. 2002. Soil ciliates (Protozoa, Ciliophora) from Namibia (Southwest Africa) with emphasis on two contrasting environments, the Etosha region and the Namib Desert. Denisia. 5: 1 - 1459." type="journal article" year="2002">Foissner et al., 2002: 312</bibRefCitation>
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</taxonomicName>
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, figs 70a–m, 329a–u
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</paragraph>
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<subSubSection id="C3036502FFAEFFAD678FFEACFD7237B2" lastPageId="21" lastPageNumber="959" pageId="18" pageNumber="956" type="description">
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<paragraph id="8BA63689FFAEFFAA678FFEACFDEE361D" blockId="18.[160,1156,298,1502]" box="[160,584,298,323]" pageId="18" pageNumber="956">
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<emphasis id="B96DEA9BFFAEFFAA678FFEACFDEE361D" bold="true" box="[160,584,298,323]" italics="true" pageId="18" pageNumber="956">Description of a Korean population</emphasis>
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</paragraph>
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<paragraph id="8BA63689FFAEFFAA678FFEC8FDCF3360" blockId="18.[160,1156,298,1502]" pageId="18" pageNumber="956">
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Body size 135–210 × 20–40 µm
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<emphasis id="B96DEA9BFFAEFFAA653EFECBFDFC3638" box="[529,602,333,358]" italics="true" pageId="18" pageNumber="956">in vivo</emphasis>
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, usually about 155 × 30 µm; not contractile but very flexible. Shape narrowly to very narrowly spatulate with a length:width ratio of 3.8– 6.8:
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1
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<emphasis id="B96DEA9BFFAEFFAA67F2FE14FF5736F5" box="[221,241,402,427]" italics="true" pageId="18" pageNumber="956">in</emphasis>
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</quantity>
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vivo and 2.7–6.1:1 after protargol impregnation; oral region inconspicuously set off from cylindrical trunk because neck only slightly narrowed; anterior end about as wide as broadest post-oral body portion; posterior end rounded (
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<figureCitation id="13222A0CFFAEFFAA64A3FE5EFC5236AE" box="[908,1012,472,496]" captionStart="Figure 8" captionStartId="19.[160,225,1556,1579]" captionTargetBox="[163,1153,142,1525]" captionTargetId="figure-1@19.[163,1153,142,1525]" captionTargetPageId="19" captionText="Figure 8. Spathidium polynucleatum from life (a, c, g) and after protargol impregnation (b, d–f). (a) Right side view of a representative individual; (b) developing cytoplasmic extrusomes, 4–8 µm long; (c) oral extrusomes, 8–10 µm long; (d, e) right and left side view of ciliary pattern and nuclear apparatus of a representative specimen; (f) dorsal view of ciliary pattern in anterior body portion; (g) cortical granulation. CG, cortical granules; CK, circumoral kinety; DB, dorsal brush; MA, macronuclear nodules/macronucleus; MI, micronuclei/micronucleus; OB, oral bulge; SC, somatic cilia; SK, somatic kinety/somatic kineties. Scale bars: a, d, e = 100 µm; f = 30 µm." figureDoi="http://doi.org/10.5281/zenodo.5181153" httpUri="https://zenodo.org/record/5181153/files/figure.png" pageId="18" pageNumber="956">Figures 8</figureCitation>
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(a,d–f) and 9 (a,b,h–j);
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<tableCitation id="C69B0332FFAEFFAA6622FE7DFEC0354D" box="[269,358,507,531]" captionStart="Table 2" captionStartId="7.[160,216,142,165]" captionText="Table 2. Morphometric data of Korean populations of Enchelys megaspinata sp. nov. (EM), Spathidium ascendens (SA), S. papilliferum (SPa, population 2), S. polynucleatum comb. nov. (SPo), S. rectitoratum (SR), and Apobryophyllum schmidingeri (AS)." pageId="18" pageNumber="956">Table 2</tableCitation>
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). Nuclear apparatus scattered throughout trunk and composed of 29–50 macronuclear nodules and 13–34 micronuclei. Macronuclear nodules oblong and about 4.5–17.0 × 3.0–6.5 µm in size after protargol impregnation; nucleoli small, globular, evenly distributed over macronuclear nodules, well recognisable after protargol impregnation. Micronuclei globular and about 1.5–2.0 µm in diameter in protargol preparations (
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<figureCitation id="13222A0CFFAEFFAA666EFD2EFE0A359E" box="[321,428,680,704]" captionStart="Figure 8" captionStartId="19.[160,225,1556,1579]" captionTargetBox="[163,1153,142,1525]" captionTargetId="figure-1@19.[163,1153,142,1525]" captionTargetPageId="19" captionText="Figure 8. Spathidium polynucleatum from life (a, c, g) and after protargol impregnation (b, d–f). (a) Right side view of a representative individual; (b) developing cytoplasmic extrusomes, 4–8 µm long; (c) oral extrusomes, 8–10 µm long; (d, e) right and left side view of ciliary pattern and nuclear apparatus of a representative specimen; (f) dorsal view of ciliary pattern in anterior body portion; (g) cortical granulation. CG, cortical granules; CK, circumoral kinety; DB, dorsal brush; MA, macronuclear nodules/macronucleus; MI, micronuclei/micronucleus; OB, oral bulge; SC, somatic cilia; SK, somatic kinety/somatic kineties. Scale bars: a, d, e = 100 µm; f = 30 µm." figureDoi="http://doi.org/10.5281/zenodo.5181153" httpUri="https://zenodo.org/record/5181153/files/figure.png" pageId="18" pageNumber="956">Figures 8</figureCitation>
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(d) and 9(e,h–j)). A single contractile vacuole at posterior end, about 13 µm across during diastole (
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<figureCitation id="13222A0CFFAEFFAA6511FD4DFD3A35BD" box="[574,668,715,739]" captionStart="Figure 9" captionStartId="21.[160,225,1462,1485]" captionText="Figure 9. Spathidium polynucleatum from life (a–g) and after protargol impregnation (h–l). (a) Right side view of a representative individual; (b) detail of posterior body portion showing contractile vacuole; (c) cortical granulation; (d, e) optical sections showing mature rod-like extrusomes and macronuclear nodules; (f) detail of anterior body end showing extrusomes attached to oral bulge; (g) epibiotic bacteria; (h–i) variability of body shape and size as well as of nuclear apparatus; (k) detail of anterior body portion showing three-rowed dorsal brush; (l) optical section showing developing fusiform extrusomes and macronuclear nodules. BA, bacteria; CV, contractile vacuole; DB, dorsal brush; EX, extrusomes; MA, macronuclear nodules/macronucleus; N, nematodesmata. Scale bars: a, h–j = 100 µm; b, f, k = 10 µm; c–e, g, l = 5 µm." pageId="18" pageNumber="956">Figure 9</figureCitation>
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(b)). Extrusomes attached to oral bulge and scattered throughout cytoplasm: oral extrusomes rod-shaped, sometimes slightly curved, 8–10 × 0.5 µm in size, do not impregnate with the protargol method used; developing cytoplasmic extrusomes fusiform, 6.5–9 µm long, middle globular part about 1.2–2.8 µm wide, impregnate deeply with the protargol method used (
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<figureCitation id="13222A0CFFAEFFAA6494FCD0FB853430" box="[955,1059,854,878]" captionStart="Figure 8" captionStartId="19.[160,225,1556,1579]" captionTargetBox="[163,1153,142,1525]" captionTargetId="figure-1@19.[163,1153,142,1525]" captionTargetPageId="19" captionText="Figure 8. Spathidium polynucleatum from life (a, c, g) and after protargol impregnation (b, d–f). (a) Right side view of a representative individual; (b) developing cytoplasmic extrusomes, 4–8 µm long; (c) oral extrusomes, 8–10 µm long; (d, e) right and left side view of ciliary pattern and nuclear apparatus of a representative specimen; (f) dorsal view of ciliary pattern in anterior body portion; (g) cortical granulation. CG, cortical granules; CK, circumoral kinety; DB, dorsal brush; MA, macronuclear nodules/macronucleus; MI, micronuclei/micronucleus; OB, oral bulge; SC, somatic cilia; SK, somatic kinety/somatic kineties. Scale bars: a, d, e = 100 µm; f = 30 µm." figureDoi="http://doi.org/10.5281/zenodo.5181153" httpUri="https://zenodo.org/record/5181153/files/figure.png" pageId="18" pageNumber="956">Figures 8</figureCitation>
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(b,c) and 9(d,f,l)). Cortex very flexible, contains about four granule rows between adjacent kineties; granules ordinarily to narrowly spaced, 0.5 µm across
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<emphasis id="B96DEA9BFFAEFFAA6432FC1CFCCF34ED" box="[797,873,922,947]" italics="true" pageId="18" pageNumber="956">in vivo</emphasis>
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; covered with epibiotic, 2 µm-long bacteria (
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<figureCitation id="13222A0CFFAEFFAA66A4FC38FE523488" box="[395,500,958,982]" captionStart="Figure 8" captionStartId="19.[160,225,1556,1579]" captionTargetBox="[163,1153,142,1525]" captionTargetId="figure-1@19.[163,1153,142,1525]" captionTargetPageId="19" captionText="Figure 8. Spathidium polynucleatum from life (a, c, g) and after protargol impregnation (b, d–f). (a) Right side view of a representative individual; (b) developing cytoplasmic extrusomes, 4–8 µm long; (c) oral extrusomes, 8–10 µm long; (d, e) right and left side view of ciliary pattern and nuclear apparatus of a representative specimen; (f) dorsal view of ciliary pattern in anterior body portion; (g) cortical granulation. CG, cortical granules; CK, circumoral kinety; DB, dorsal brush; MA, macronuclear nodules/macronucleus; MI, micronuclei/micronucleus; OB, oral bulge; SC, somatic cilia; SK, somatic kinety/somatic kineties. Scale bars: a, d, e = 100 µm; f = 30 µm." figureDoi="http://doi.org/10.5281/zenodo.5181153" httpUri="https://zenodo.org/record/5181153/files/figure.png" pageId="18" pageNumber="956">Figures 8</figureCitation>
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(g) and 9(c,g)). Cytoplasm colourless, packed with macronuclear nodules, developing fusiform extrusomes, and 2–8-µm-sized lipid droplets (
|
||
<figureCitation id="13222A0CFFAEFFAA6787FB85FEA93345" box="[168,271,1027,1051]" captionStart="Figure 9" captionStartId="21.[160,225,1462,1485]" captionText="Figure 9. Spathidium polynucleatum from life (a–g) and after protargol impregnation (h–l). (a) Right side view of a representative individual; (b) detail of posterior body portion showing contractile vacuole; (c) cortical granulation; (d, e) optical sections showing mature rod-like extrusomes and macronuclear nodules; (f) detail of anterior body end showing extrusomes attached to oral bulge; (g) epibiotic bacteria; (h–i) variability of body shape and size as well as of nuclear apparatus; (k) detail of anterior body portion showing three-rowed dorsal brush; (l) optical section showing developing fusiform extrusomes and macronuclear nodules. BA, bacteria; CV, contractile vacuole; DB, dorsal brush; EX, extrusomes; MA, macronuclear nodules/macronucleus; N, nematodesmata. Scale bars: a, h–j = 100 µm; b, f, k = 10 µm; c–e, g, l = 5 µm." pageId="18" pageNumber="956">Figures 9</figureCitation>
|
||
(a,d,e)). Movement relatively slow, either gliding on microscope slide or swimming by rotation about main body axis.
|
||
</paragraph>
|
||
<paragraph id="8BA63689FFAEFFAA67EFFBCEFF58326E" blockId="18.[160,1156,298,1502]" pageId="18" pageNumber="956">
|
||
Cilia 8–11 µm long
|
||
<emphasis id="B96DEA9BFFAEFFAA668FFBC1FE4F333E" box="[416,489,1095,1120]" italics="true" pageId="18" pageNumber="956">in vivo</emphasis>
|
||
, spaced ordinarily except for five to seven densely spaced basal bodies in curved anterior kinety portion. On average 24 equidistant, ordinarily spaced meridional ciliary rows anteriorly curved distinctly dorsally on right side while curved slightly to distinctly ventrally on left side. Dorsal brush dikinetidal; three-rowed, isostichad; middle row slightly longer than right and left row; dikinetids more narrowly spaced in anterior (1 µm) than posterior (2–2.5 µm) brush portion (
|
||
<figureCitation id="13222A0CFFAEFFAA64AAFB70FC4A3250" box="[901,1004,1270,1294]" captionStart="Figure 8" captionStartId="19.[160,225,1556,1579]" captionTargetBox="[163,1153,142,1525]" captionTargetId="figure-1@19.[163,1153,142,1525]" captionTargetPageId="19" captionText="Figure 8. Spathidium polynucleatum from life (a, c, g) and after protargol impregnation (b, d–f). (a) Right side view of a representative individual; (b) developing cytoplasmic extrusomes, 4–8 µm long; (c) oral extrusomes, 8–10 µm long; (d, e) right and left side view of ciliary pattern and nuclear apparatus of a representative specimen; (f) dorsal view of ciliary pattern in anterior body portion; (g) cortical granulation. CG, cortical granules; CK, circumoral kinety; DB, dorsal brush; MA, macronuclear nodules/macronucleus; MI, micronuclei/micronucleus; OB, oral bulge; SC, somatic cilia; SK, somatic kinety/somatic kineties. Scale bars: a, d, e = 100 µm; f = 30 µm." figureDoi="http://doi.org/10.5281/zenodo.5181153" httpUri="https://zenodo.org/record/5181153/files/figure.png" pageId="18" pageNumber="956">Figures 8</figureCitation>
|
||
(d–f) and 9(k);
|
||
<tableCitation id="C69B0332FFAEFFAA678FFA9EFF57326E" box="[160,241,1304,1328]" captionStart="Table 2" captionStartId="7.[160,216,142,165]" captionText="Table 2. Morphometric data of Korean populations of Enchelys megaspinata sp. nov. (EM), Spathidium ascendens (SA), S. papilliferum (SPa, population 2), S. polynucleatum comb. nov. (SPo), S. rectitoratum (SR), and Apobryophyllum schmidingeri (AS)." pageId="18" pageNumber="956">Table 2</tableCitation>
|
||
).
|
||
</paragraph>
|
||
<paragraph id="8BA63689FFAEFFAA67EFFABDFCA73280" blockId="18.[160,1156,298,1502]" pageId="18" pageNumber="956">
|
||
Oral bulge occupies ordinarily oblique anterior body end, distinctly set off from body proper and comparatively thick (
|
||
<figureCitation id="13222A0CFFAEFFAA6530FAD8FDD93228" box="[543,639,1374,1398]" captionStart="Figure 9" captionStartId="21.[160,225,1462,1485]" captionText="Figure 9. Spathidium polynucleatum from life (a–g) and after protargol impregnation (h–l). (a) Right side view of a representative individual; (b) detail of posterior body portion showing contractile vacuole; (c) cortical granulation; (d, e) optical sections showing mature rod-like extrusomes and macronuclear nodules; (f) detail of anterior body end showing extrusomes attached to oral bulge; (g) epibiotic bacteria; (h–i) variability of body shape and size as well as of nuclear apparatus; (k) detail of anterior body portion showing three-rowed dorsal brush; (l) optical section showing developing fusiform extrusomes and macronuclear nodules. BA, bacteria; CV, contractile vacuole; DB, dorsal brush; EX, extrusomes; MA, macronuclear nodules/macronucleus; N, nematodesmata. Scale bars: a, h–j = 100 µm; b, f, k = 10 µm; c–e, g, l = 5 µm." pageId="18" pageNumber="956">Figure 9</figureCitation>
|
||
(a)). Circumoral kinety at base of oral bulge, continuous, composed of narrowly spaced and perpendicularly oriented dikinetids associated with nematodesmata, forming easily recognisable bundles in some protargol-impregnated specimens (
|
||
<figureCitation id="13222A0CFFAEFFAA66C2FA40FDF03280" box="[493,598,1478,1502]" captionStart="Figure 8" captionStartId="19.[160,225,1556,1579]" captionTargetBox="[163,1153,142,1525]" captionTargetId="figure-1@19.[163,1153,142,1525]" captionTargetPageId="19" captionText="Figure 8. Spathidium polynucleatum from life (a, c, g) and after protargol impregnation (b, d–f). (a) Right side view of a representative individual; (b) developing cytoplasmic extrusomes, 4–8 µm long; (c) oral extrusomes, 8–10 µm long; (d, e) right and left side view of ciliary pattern and nuclear apparatus of a representative specimen; (f) dorsal view of ciliary pattern in anterior body portion; (g) cortical granulation. CG, cortical granules; CK, circumoral kinety; DB, dorsal brush; MA, macronuclear nodules/macronucleus; MI, micronuclei/micronucleus; OB, oral bulge; SC, somatic cilia; SK, somatic kinety/somatic kineties. Scale bars: a, d, e = 100 µm; f = 30 µm." figureDoi="http://doi.org/10.5281/zenodo.5181153" httpUri="https://zenodo.org/record/5181153/files/figure.png" pageId="18" pageNumber="956">Figures 8</figureCitation>
|
||
(d–f) and 9(i,j)).
|
||
</paragraph>
|
||
<paragraph id="8BA63689FFAEFFAA678FF98CFD5B317D" blockId="18.[160,1156,1546,1710]" box="[160,765,1546,1571]" pageId="18" pageNumber="956">
|
||
<emphasis id="B96DEA9BFFAEFFAA678FF98CFD5B317D" bold="true" box="[160,765,1546,1571]" italics="true" pageId="18" pageNumber="956">Comparison with original description and remarks</emphasis>
|
||
</paragraph>
|
||
<paragraph id="8BA63689FFAEFFAA678FF9A8FB2231F0" blockId="18.[160,1156,1546,1710]" pageId="18" pageNumber="956">
|
||
The Korean population matches well the Namibian
|
||
<typeStatus id="54A2882BFFAEFFAA643DF9A8FCE23118" box="[786,836,1582,1606]" pageId="18" pageNumber="956">type</typeStatus>
|
||
population and Australian population with regard to body size and shape, macronuclear and extrusome pattern, as well as the number of somatic ciliary rows (
|
||
<bibRefCitation id="EF884B78FFAEFFAA6587F9F5FC2431D5" author="Foissner W & Agatha S & Berger H" box="[680,898,1651,1675]" pageId="18" pageNumber="956" pagination="1 - 1459" refId="ref14095" refString="Foissner W, Agatha S, Berger H. 2002. Soil ciliates (Protozoa, Ciliophora) from Namibia (Southwest Africa) with emphasis on two contrasting environments, the Etosha region and the Namib Desert. Denisia. 5: 1 - 1459." type="journal article" year="2002">Foissner et al. 2002</bibRefCitation>
|
||
). Conspecificity is thus beyond reasonable doubt. Because its oral ciliary pattern is in transition between
|
||
</paragraph>
|
||
<caption id="DF666601FFAFFFAB678FF992FC8C3185" ID-DOI="http://doi.org/10.5281/zenodo.5181153" ID-Zenodo-Dep="5181153" httpUri="https://zenodo.org/record/5181153/files/figure.png" pageId="19" pageNumber="957" startId="19.[160,225,1556,1579]" targetBox="[163,1153,142,1525]" targetPageId="19">
|
||
<paragraph id="8BA63689FFAFFFAB678FF992FC8C3185" blockId="19.[160,1156,1555,1755]" pageId="19" pageNumber="957">
|
||
<emphasis id="B96DEA9BFFAFFFAB678FF992FF5A3175" bold="true" box="[160,252,1556,1579]" pageId="19" pageNumber="957">Figure 8.</emphasis>
|
||
<taxonomicName id="4C194D0AFFAFFFAB662BF995FDA13174" authorityName="Jang & Vďačný & Shazib & Shin" authorityYear="2017" baseAuthorityName="Jang & Vďačný & Shazib & Shin" baseAuthorityYear="2002" box="[260,519,1555,1578]" class="Gymnostomatea" family="Spathidiidae" genus="Spathidium" kingdom="Chromista" order="Spathidiida" pageId="19" pageNumber="957" phylum="Ciliophora" rank="species" species="polynucleatum">
|
||
<emphasis id="B96DEA9BFFAFFFAB662BF995FDA13174" box="[260,519,1555,1578]" italics="true" pageId="19" pageNumber="957">Spathidium polynucleatum</emphasis>
|
||
</taxonomicName>
|
||
from life (a, c, g) and after protargol impregnation (b, d–f). (a) Right side view of a representative individual; (b) developing cytoplasmic extrusomes, 4–8 µm long; (c) oral extrusomes, 8–10 µm long; (d, e) right and left side view of ciliary pattern and nuclear apparatus of a representative specimen; (f) dorsal view of ciliary pattern in anterior body portion; (g) cortical granulation. CG, cortical granules; CK, circumoral kinety; DB, dorsal brush; MA, macronuclear nodules/macronucleus; MI, micronuclei/micronucleus; OB, oral bulge; SC, somatic cilia; SK, somatic kinety/somatic kineties. Scale bars: a, d, e = 100 µm; f = 30 µm.
|
||
</paragraph>
|
||
</caption>
|
||
<paragraph id="8BA63689FFA9FFAD678FFF0BFD7237B2" blockId="21.[160,1155,141,236]" pageId="21" pageNumber="959">
|
||
<taxonomicName id="4C194D0AFFA9FFAD678FFF0BFEBB37F8" authorityName="Dujardin" authorityYear="1841" box="[160,285,141,166]" class="Gymnostomatea" family="Spathidiidae" genus="Spathidium" kingdom="Chromista" order="Spathidiida" pageId="21" pageNumber="959" phylum="Ciliophora" rank="genus">
|
||
<emphasis id="B96DEA9BFFA9FFAD678FFF0BFEBB37F8" box="[160,285,141,166]" italics="true" pageId="21" pageNumber="959">Spathidium</emphasis>
|
||
</taxonomicName>
|
||
- and
|
||
<taxonomicName id="4C194D0AFFA9FFAD664DFF0BFE5B37F8" authorityName="Foissner" authorityYear="1984" box="[354,509,141,166]" class="Gymnostomatea" family="Spathidiidae" genus="Epispathidium" kingdom="Chromista" order="Spathidiida" pageId="21" pageNumber="959" phylum="Ciliophora" rank="genus">
|
||
<emphasis id="B96DEA9BFFA9FFAD664DFF0BFE5B37F8" box="[354,509,141,166]" italics="true" pageId="21" pageNumber="959">Epispathidium</emphasis>
|
||
</taxonomicName>
|
||
-like, as already mentioned by
|
||
<bibRefCitation id="EF884B78FFA9FFAD6471FF08FBED37F8" author="Foissner W & Agatha S & Berger H" box="[862,1099,142,166]" pageId="21" pageNumber="959" pagination="1 - 1459" refId="ref14095" refString="Foissner W, Agatha S, Berger H. 2002. Soil ciliates (Protozoa, Ciliophora) from Namibia (Southwest Africa) with emphasis on two contrasting environments, the Etosha region and the Namib Desert. Denisia. 5: 1 - 1459." type="journal article" year="2002">Foissner et al. (2002)</bibRefCitation>
|
||
, and because it clusters with other
|
||
<taxonomicName id="4C194D0AFFA9FFAD66D7FF36FD683797" box="[504,718,176,201]" class="Gymnostomatea" family="Spathidiidae" genus="Spathidium" kingdom="Chromista" order="Spathidiida" pageId="21" pageNumber="959" phylum="Ciliophora" rank="species" species="undetermined">
|
||
<emphasis id="B96DEA9BFFA9FFAD66D7FF36FDD33797" box="[504,629,176,201]" italics="true" pageId="21" pageNumber="959">Spathidium</emphasis>
|
||
species
|
||
</taxonomicName>
|
||
in molecular phylogenies, we suggest transferring this species to the genus
|
||
<taxonomicName id="4C194D0AFFA9FFAD657DFF55FD6937B2" authorityName="Dujardin" authorityYear="1841" box="[594,719,211,236]" class="Gymnostomatea" family="Spathidiidae" genus="Spathidium" kingdom="Chromista" order="Spathidiida" pageId="21" pageNumber="959" phylum="Ciliophora" rank="genus">
|
||
<emphasis id="B96DEA9BFFA9FFAD657DFF55FD6937B2" box="[594,719,211,236]" italics="true" pageId="21" pageNumber="959">Spathidium</emphasis>
|
||
</taxonomicName>
|
||
.
|
||
</paragraph>
|
||
</subSubSection>
|
||
</treatment>
|
||
</document> |