312 lines
41 KiB
XML
312 lines
41 KiB
XML
<document id="64CEBBC1ED2DF8BE1A8D5F5ADD845055" ID-CLB-Dataset="293780" ID-DOI="10.1071/SB22010" ID-GBIF-Dataset="33b90653-44a4-4906-840e-526fbf251b44" ID-ISSN="1446-5701" ID-Zenodo-Dep="10974463" IM.bibliography_approvedBy="guilherme" IM.illustrations_approvedBy="guilherme" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.taxonomicNames_approvedBy="guilherme" IM.treatments_approvedBy="guilherme" checkinTime="1711993764777" checkinUser="felipe" docAuthor="Schmidt-Lebuhn, Alexander N." docDate="2022" docId="052287BADD38FFF4103DF9B2FC2117DE" docLanguage="en" docName="AustSystBot.35.4.SB22010.pdf" docOrigin="Australian Systematic Botany 35 (4)" docSource="http://dx.doi.org/10.1071/sb22010" docStyle="DocumentStyle:CD0BCB9C444EA940AA05010D9A9F3556.2:AustSystBot.2022-.journal_article.1cover" docStyleId="CD0BCB9C444EA940AA05010D9A9F3556" docStyleName="AustSystBot.2022-.journal_article.1cover" docStyleVersion="2" docTitle="Pogonolepis Steetz" docType="treatment" docVersion="3" lastPageNumber="323" masterDocId="F91BFFC2DD3EFFF3105FFFF2FFB81259" masterDocTitle="Sequence capture data support the taxonomy of Pogonolepis (Asteraceae: Gnaphalieae) and show unexpected genetic structure" masterLastPageNumber="325" masterPageNumber="317" pageNumber="322" updateTime="1713190322591" updateUser="ExternalLinkService">
|
||
<mods:mods id="C2F30DE498EFDF43066F5E0995E91C69" xmlns:mods="http://www.loc.gov/mods/v3">
|
||
<mods:titleInfo id="A2812D88C709979099CE2C0C802471BB">
|
||
<mods:title id="EFEB62C319EDBA78ABDA183EB6168970">Sequence capture data support the taxonomy of Pogonolepis (Asteraceae: Gnaphalieae) and show unexpected genetic structure</mods:title>
|
||
</mods:titleInfo>
|
||
<mods:name id="E98167A3A166CDBBF7B47B907E754990" type="personal">
|
||
<mods:role id="D9E57F3E98B4621282B11ED49687FD70">
|
||
<mods:roleTerm id="034269CBB8EADEBA036F5ACC1118DB8B">Author</mods:roleTerm>
|
||
</mods:role>
|
||
<mods:namePart id="AB7966FB97FC15C5CB16AE5B09398FD5">Schmidt-Lebuhn, Alexander N.</mods:namePart>
|
||
</mods:name>
|
||
<mods:typeOfResource id="4DB0972F5B530DD9CA465A0BD9C126BF">text</mods:typeOfResource>
|
||
<mods:relatedItem id="51FBD3455AD3A7322B958B4C0499425A" type="host">
|
||
<mods:titleInfo id="5014821A2FB3E300A5A7C51B774A7867">
|
||
<mods:title id="CE4609F010869D7D17347EFC6464153C">Australian Systematic Botany</mods:title>
|
||
</mods:titleInfo>
|
||
<mods:part id="A20B3B5A4A557C55378666647BA244C4">
|
||
<mods:date id="940D7A701CB8AC3826544A13E4EFD008">2022</mods:date>
|
||
<mods:detail id="AD737A4E3D3DC9B06DF579F104A8330C" type="pubDate">
|
||
<mods:number id="A0B51630CC98FA9F9B4E516CB9CCCBEC">2022-08-25</mods:number>
|
||
</mods:detail>
|
||
<mods:detail id="146832B3965BCEE5E706B56F78E34973" type="volume">
|
||
<mods:number id="32AC99090A41F82E8BD38A6C60DA4CB1">35</mods:number>
|
||
</mods:detail>
|
||
<mods:detail id="C43B6D1E7C4F49DE4DF08A1A29226642" type="issue">
|
||
<mods:number id="4A433703DF37BB8968156BCE92B7353A">4</mods:number>
|
||
</mods:detail>
|
||
<mods:extent id="FE76772B5AE359908A7805A7D8007306" unit="page">
|
||
<mods:start id="5D176438D70A28B3D8B2E7E27191249C">317</mods:start>
|
||
<mods:end id="E0DCC067D01F02BCDB9BF71A684AFED4">325</mods:end>
|
||
</mods:extent>
|
||
</mods:part>
|
||
</mods:relatedItem>
|
||
<mods:location id="E4EFABF493215B9D2C61B541576248CA">
|
||
<mods:url id="3570F8E24B00AF8B36823C97334B2C9A">http://dx.doi.org/10.1071/sb22010</mods:url>
|
||
</mods:location>
|
||
<mods:classification id="B32CCC0C20A6140DCE646AF4E48D73D6">journal article</mods:classification>
|
||
<mods:identifier id="9386467D0CDA06F882B35F1EC7FA228D" type="CLB-Dataset">293780</mods:identifier>
|
||
<mods:identifier id="31B3895CE1921FD07EC1350E2FDE40C3" type="DOI">10.1071/SB22010</mods:identifier>
|
||
<mods:identifier id="1ACCA5EE7E58B9128C8EE146140D67A6" type="GBIF-Dataset">33b90653-44a4-4906-840e-526fbf251b44</mods:identifier>
|
||
<mods:identifier id="FA5CF28819E60049453B26BD38F054EF" type="ISSN">1446-5701</mods:identifier>
|
||
<mods:identifier id="5162CC6E09211D11C704E5E237F2DC32" type="Zenodo-Dep">10974463</mods:identifier>
|
||
</mods:mods>
|
||
<treatment id="052287BADD38FFF4103DF9B2FC2117DE" ID-DOI="http://doi.org/10.5281/zenodo.10974471" ID-Zenodo-Dep="10974471" LSID="urn:lsid:plazi:treatment:052287BADD38FFF4103DF9B2FC2117DE" httpUri="http://treatment.plazi.org/id/052287BADD38FFF4103DF9B2FC2117DE" lastPageId="7" lastPageNumber="323" pageId="6" pageNumber="322">
|
||
<subSubSection id="C5916527DD38FFF5103DF9B2FDFA1404" box="[98,578,1600,1629]" pageId="6" pageNumber="322" type="nomenclature">
|
||
<paragraph id="8D3436ACDD38FFF5103DF9B2FDFA1404" blockId="6.[98,578,1600,1629]" box="[98,578,1600,1629]" pageId="6" pageNumber="322">
|
||
<heading id="D67C81C0DD38FFF5103DF9B2FDFA1404" bold="true" box="[98,578,1600,1629]" fontSize="11" level="3" pageId="6" pageNumber="322" reason="9">
|
||
<emphasis id="BFFFEABEDD38FFF5103DF9B2FDFA1404" bold="true" box="[98,578,1600,1629]" pageId="6" pageNumber="322">
|
||
Species delimitation in
|
||
<taxonomicName id="4A8B4D2FDD38FFF511F9F9B3FDFA1404" ID-CoL="6TNF" authority="Steetz" authorityName="Steetz" box="[422,578,1601,1629]" class="Magnoliopsida" family="Asteraceae" genus="Pogonolepis" kingdom="Plantae" order="Asterales" pageId="6" pageNumber="322" phylum="Tracheophyta" rank="genus">
|
||
<emphasis id="BFFFEABEDD38FFF511F9F9B3FDFA1404" bold="true" box="[422,578,1601,1629]" italics="true" pageId="6" pageNumber="322">Pogonolepis</emphasis>
|
||
</taxonomicName>
|
||
</emphasis>
|
||
</heading>
|
||
</paragraph>
|
||
</subSubSection>
|
||
<subSubSection id="C5916527DD38FFF4103DF986FC2117DE" lastPageId="7" lastPageNumber="323" pageId="6" pageNumber="322" type="description">
|
||
<paragraph id="8D3436ACDD38FFF5103DF986FA701335" blockId="6.[98,770,1652,1932]" lastBlockId="6.[818,1490,180,1356]" pageId="6" pageNumber="322">
|
||
As implied by the above, molecular data confirmed
|
||
<bibRefCitation id="E91A4B5DDD38FFF512E7F986FF1414F5" author="Short PS" pageId="6" pageNumber="322" pagination="237 - 253" refId="ref6179" refString="Short PS (1986) A revision of Pogonolepis (Compositae: Inuleae: Gnaphaliinae). Muelleria 6, 237 - 253. doi: 10.5962 / p. 184052" type="journal article" year="1986">Short’s (1986)</bibRefCitation>
|
||
taxonomy of
|
||
<taxonomicName id="4A8B4D2FDD38FFF5111FF966FE7914F5" box="[320,449,1684,1708]" class="Magnoliopsida" family="Asteraceae" genus="Pogonolepis" kingdom="Plantae" order="Asterales" pageId="6" pageNumber="322" phylum="Tracheophyta" rank="genus">
|
||
<emphasis id="BFFFEABEDD38FFF5111FF966FE7914F5" bold="true" box="[320,449,1684,1708]" italics="true" pageId="6" pageNumber="322">Pogonolepis</emphasis>
|
||
</taxonomicName>
|
||
, but not his hypothesis of the polyphyletic origin of
|
||
<taxonomicName id="4A8B4D2FDD38FFF5110FF946FE561495" box="[336,494,1716,1740]" class="Magnoliopsida" family="Asteraceae" genus="Pogonolepis" kingdom="Plantae" order="Asterales" pageId="6" pageNumber="322" phylum="Tracheophyta" rank="species" species="muelleriana">
|
||
<emphasis id="BFFFEABEDD38FFF5110FF946FE561495" bold="true" box="[336,494,1716,1740]" italics="true" pageId="6" pageNumber="322">P. muelleriana</emphasis>
|
||
</taxonomicName>
|
||
suggested by its diversity in chromosome numbers. On current evidence, it appears that extant populations of
|
||
<taxonomicName id="4A8B4D2FDD38FFF5110DF906FE4B1555" box="[338,499,1780,1804]" class="Magnoliopsida" family="Asteraceae" genus="Pogonolepis" kingdom="Plantae" order="Asterales" pageId="6" pageNumber="322" phylum="Tracheophyta" rank="species" species="muelleriana">
|
||
<emphasis id="BFFFEABEDD38FFF5110DF906FE4B1555" bold="true" box="[338,499,1780,1804]" italics="true" pageId="6" pageNumber="322">P. muelleriana</emphasis>
|
||
</taxonomicName>
|
||
and
|
||
<taxonomicName id="4A8B4D2FDD38FFF51274F906FD361555" box="[555,654,1780,1804]" class="Magnoliopsida" family="Asteraceae" genus="Pogonolepis" kingdom="Plantae" order="Asterales" pageId="6" pageNumber="322" phylum="Tracheophyta" rank="species" species="stricta">
|
||
<emphasis id="BFFFEABEDD38FFF51274F906FD361555" bold="true" box="[555,654,1780,1804]" italics="true" pageId="6" pageNumber="322">P. stricta</emphasis>
|
||
</taxonomicName>
|
||
constitute two sister lineages, and that the former may have arisen from a single event. However, clade support for
|
||
<taxonomicName id="4A8B4D2FDD38FFF5126CF8C6FD2C1515" box="[563,660,1844,1868]" class="Magnoliopsida" family="Asteraceae" genus="Pogonolepis" kingdom="Plantae" order="Asterales" pageId="6" pageNumber="322" phylum="Tracheophyta" rank="species" species="stricta">
|
||
<emphasis id="BFFFEABEDD38FFF5126CF8C6FD2C1515" bold="true" box="[563,660,1844,1868]" italics="true" pageId="6" pageNumber="322">P. stricta</emphasis>
|
||
</taxonomicName>
|
||
is considerably lower than for its sister, potentially reflecting a lower degree of lineage sorting since divergence of the two species. Another caveat is that geographic sampling of
|
||
<taxonomicName id="4A8B4D2FDD38FFF5156DFF46FA6A1295" box="[1330,1490,180,204]" class="Magnoliopsida" family="Asteraceae" genus="Pogonolepis" kingdom="Plantae" order="Asterales" pageId="6" pageNumber="322" phylum="Tracheophyta" rank="species" species="muelleriana">
|
||
<emphasis id="BFFFEABEDD38FFF5156DFF46FA6A1295" bold="true" box="[1330,1490,180,204]" italics="true" pageId="6" pageNumber="322">P. muelleriana</emphasis>
|
||
</taxonomicName>
|
||
in
|
||
<collectingRegion id="4F4FF84EDD38FFF5130EFF26FBAF12B5" box="[849,1047,212,236]" country="Australia" name="Western Australia" pageId="6" pageNumber="322">Western Australia</collectingRegion>
|
||
was limited to the southern half of its distribution in that state (
|
||
<figureCitation id="15B02A29DD38FFF5140CFF06FB2B1355" box="[1107,1171,244,268]" captionStart="Fig" captionStartId="3.[1103,1140,1615,1638]" captionTargetBox="[151,1057,977,1878]" captionTargetId="figure-348@3.[145,1058,977,1884]" captionTargetPageId="3" captionText="Fig. 2. Geographic spread of the specimens of Pogonolepis at CANB sampled for molecular analysis (large, pale circles) and ranges of species according to the Australasian Virtual Herbarium (dots; see https://doi.org/10.26197/ala.6556e234- 7160-49de-bf63-5123af624e94, accessed 31 May 2022). Red: P. muelleriana; blue: P. stricta. Note that specimens of P. muel- 150 leriana geocoded in Canberra and Hobart were likely cultivated." figureDoi="http://doi.org/10.5281/zenodo.10974467" httpUri="https://zenodo.org/record/10974467/files/figure.png" pageId="6" pageNumber="322">Fig. 2</figureCitation>
|
||
), so it remains possible that broader sampling may yet show multiple origins. However, it appears highly likely that all its eastern Australian populations form a clade, because they are well represented in the data.
|
||
</paragraph>
|
||
<paragraph id="8D3436ACDD38FFF5130DFE86FC7810F5" blockId="6.[818,1490,180,1356]" pageId="6" pageNumber="322">
|
||
This result intuitively supports the continued recognition of
|
||
<taxonomicName id="4A8B4D2FDD38FFF5130CFE66FC4013F2" box="[851,1016,403,427]" class="Magnoliopsida" family="Asteraceae" genus="Pogonolepis" kingdom="Plantae" order="Asterales" pageId="6" pageNumber="322" phylum="Tracheophyta" rank="species" species="muelleriana">
|
||
<emphasis id="BFFFEABEDD38FFF5130CFE66FC4013F2" bold="true" box="[851,1016,403,427]" italics="true" pageId="6" pageNumber="322">P. muelleriana</emphasis>
|
||
</taxonomicName>
|
||
and
|
||
<taxonomicName id="4A8B4D2FDD38FFF51465FE66FB1813F5" box="[1082,1184,404,428]" class="Magnoliopsida" family="Asteraceae" genus="Pogonolepis" kingdom="Plantae" order="Asterales" pageId="6" pageNumber="322" phylum="Tracheophyta" rank="species" species="stricta">
|
||
<emphasis id="BFFFEABEDD38FFF51465FE66FB1813F5" bold="true" box="[1082,1184,404,428]" italics="true" pageId="6" pageNumber="322">P. stricta</emphasis>
|
||
</taxonomicName>
|
||
as species. However, it is desirable to make methodological and taxonomic approaches transparent. In the present case, the existence in the
|
||
<taxonomicName id="4A8B4D2FDD38FFF5136DFE06FC161055" box="[818,942,500,524]" class="Magnoliopsida" family="Asteraceae" genus="Angianthus" kingdom="Plantae" order="Asterales" pageId="6" pageNumber="322" phylum="Tracheophyta" rank="genus">Angianthus</taxonomicName>
|
||
clade of other pairs differing only in reproductive system (
|
||
<bibRefCitation id="E91A4B5DDD38FFF513D2FDE6FBBE1075" author="Short PS" box="[909,1030,532,556]" pageId="6" pageNumber="322" pagination="9 - 22" refId="ref6144" refString="Short PS (1985) A revision of Actinobole Fenzl ex Endl. (Compositae: Inuleae: Gnaphaliinae). Muelleria 6, 9 - 22. doi: 10.5962 / p. 184058" type="journal article" year="1985">Short 1985</bibRefCitation>
|
||
) and of various other genera with combinations of sexually and asexually reproducing species (
|
||
<bibRefCitation id="E91A4B5DDD38FFF515C8FDC6FCD21035" author="Short PS" pageId="6" pageNumber="322" pagination="143 - 183" refId="ref6106" refString="Short PS (1983) A revision of Angianthus Wendl., sensu lato (Compositae: Inuleae: Gnaphaliinae), 1. Muelleria 5, 143 - 183. doi: 10.5962 / p. 238385" type="journal article" year="1983">Short 1983</bibRefCitation>
|
||
,
|
||
<bibRefCitation id="E91A4B5DDD38FFF51323FDA6FC0C1035" author="Short PS" box="[892,948,596,620]" pageId="6" pageNumber="322" pagination="39 - 56" refId="ref6210" refString="Short PS (1989) A revision of Podotheca Cass. (Asteraceae: Inuleae: Gnaphaliinae). Muelleria 7, 39 - 56. doi: 10.5962 / p. 184036" type="journal article" year="1989">1989</bibRefCitation>
|
||
,
|
||
<bibRefCitation id="E91A4B5DDD38FFF51399FDA6FBB31032" author="Short PS" box="[966,1035,596,620]" pageId="6" pageNumber="322" pagination="225 - 238" refId="ref6243" refString="Short PS (1990 a) A revision of the genus Chthonocephalus Steetz (Asteraceae: Inuleae: Gnaphaliinae). Muelleria 7, 225 - 238. doi: 10.5962 / p. 184026" type="journal article" year="1990">
|
||
1990
|
||
<emphasis id="BFFFEABEDD38FFF513A1FDA6FBB31032" bold="true" box="[1022,1035,596,619]" italics="true" pageId="6" pageNumber="322">a</emphasis>
|
||
</bibRefCitation>
|
||
,
|
||
<bibRefCitation id="E91A4B5DDD38FFF51440FDA6FBDA1032" author="Short PS" box="[1055,1122,595,620]" pageId="6" pageNumber="322" pagination="213 - 224" refId="ref6278" refString="Short PS (1990 b) A revision of Trichanthodium Sond. & F. Muell. ex Sond. (Asteraceae: Inuleae: Gnaphaliinae). Muelleria 7, 213 - 224. doi: 10.5962 / p. 184025" type="journal article" year="1990">
|
||
1990
|
||
<emphasis id="BFFFEABEDD38FFF51409FDA1FBDA1032" bold="true" box="[1110,1122,595,619]" italics="true" pageId="6" pageNumber="322">b</emphasis>
|
||
</bibRefCitation>
|
||
,
|
||
<bibRefCitation id="E91A4B5DDD38FFF51429FDA6FB161035" author="Short PS" box="[1142,1198,596,620]" pageId="6" pageNumber="322" pagination="147 - 220" refId="ref6417" refString="Short PS (2015) Notes concerning the classification of species included in Calocephalus R. Br. s. lat. and Gnephosis Cass. s. lat. (Asteraceae: Gnaphalieae), with descriptions of new genera and species. Journal of the Adelaide Botanic Gardens 29, 147 - 220." type="journal article" year="2015">2015</bibRefCitation>
|
||
) means that it would be logical to apply a consistent approach to species delimitation in this clade.
|
||
</paragraph>
|
||
<paragraph id="8D3436ACDD38FFF5130DFD46FA691655" blockId="6.[818,1490,180,1356]" pageId="6" pageNumber="322">
|
||
The problem remains that because the two members of each pair have different reproductive systems, naive application of a single species concept is impossible. If, for example, non-outcrossing populations are to be separated from outcrossing ones because there is no gene flow between the two entities (biological species concept), then, logically, each individual of the non-outcrossing populations would have to be its own species. This illustrates the force of arguments that species are better considered as phenomena in need of explanation, rather than a pre-existing concept into which patterns of diversity are forced (
|
||
<bibRefCitation id="E91A4B5DDD38FFF514C1FC06FA791655" author="Mishler BD & Wilkins JS" box="[1182,1473,1012,1036]" pageId="6" pageNumber="322" pagination="1" refId="ref5812" refString="Mishler BD, Wilkins JS (2018) The hunting of the SNaRC: a snarky solution to the species problem. Philosophy, Theory, and Practice in Biology 10, 1. doi: 10.3998 / ptpbio. 16039257.0010.001" type="journal article" year="2018">Mishler and Wilkins 2018</bibRefCitation>
|
||
).
|
||
</paragraph>
|
||
<paragraph id="8D3436ACDD38FFF5130DFBE6FA2C1715" blockId="6.[818,1490,180,1356]" pageId="6" pageNumber="322">
|
||
Such an approach provides the conceptual flexibility under which, in this case,
|
||
<taxonomicName id="4A8B4D2FDD38FFF5144DFBC6FBCE1615" box="[1042,1142,1076,1100]" class="Magnoliopsida" family="Asteraceae" genus="Pogonolepis" kingdom="Plantae" order="Asterales" pageId="6" pageNumber="322" phylum="Tracheophyta" rank="species" species="stricta">
|
||
<emphasis id="BFFFEABEDD38FFF5144DFBC6FBCE1615" bold="true" box="[1042,1142,1076,1100]" italics="true" pageId="6" pageNumber="322">P. stricta</emphasis>
|
||
</taxonomicName>
|
||
can be considered a biological species and
|
||
<taxonomicName id="4A8B4D2FDD38FFF513E4FBA6FBE51635" box="[955,1117,1108,1132]" class="Magnoliopsida" family="Asteraceae" genus="Pogonolepis" kingdom="Plantae" order="Asterales" pageId="6" pageNumber="322" phylum="Tracheophyta" rank="species" species="muelleriana">
|
||
<emphasis id="BFFFEABEDD38FFF513E4FBA6FBE51635" bold="true" box="[955,1117,1108,1132]" italics="true" pageId="6" pageNumber="322">P. muelleriana</emphasis>
|
||
</taxonomicName>
|
||
an agamospecies (
|
||
<bibRefCitation id="E91A4B5DDD38FFF5156BFBA6FA7A1635" author="Zachos FE" box="[1332,1474,1108,1132]" pageId="6" pageNumber="322" refId="ref6610" refString="Zachos FE (2016) ' Species Concepts in Biology. ' (Springer International Publishing) doi: 10.1007 / 978 - 3 - 319 - 44966 - 1" type="book" year="2016">Zachos 2016</bibRefCitation>
|
||
). Although it may be argued that the data presented here show the two taxa to be reciprocally monophyletic and, therefore, the concept of monophyletic species to be equally applicable, the statement that a sexually reproducing species such as
|
||
<taxonomicName id="4A8B4D2FDD38FFF5136DFB06FC2F1755" box="[818,919,1268,1292]" class="Magnoliopsida" family="Asteraceae" genus="Pogonolepis" kingdom="Plantae" order="Asterales" pageId="6" pageNumber="322" phylum="Tracheophyta" rank="species" species="stricta">
|
||
<emphasis id="BFFFEABEDD38FFF5136DFB06FC2F1755" bold="true" box="[818,919,1268,1292]" italics="true" pageId="6" pageNumber="322">P. stricta</emphasis>
|
||
</taxonomicName>
|
||
is monophyletic is a category error. Because it is sexually reproducing, there is no phylogenetic structure to be -phyletic in, be it mono-,
|
||
<typeStatus id="5230880EDD38FFF51413FAC6FB3C1712" box="[1100,1156,1332,1355]" pageId="6" pageNumber="322" type="paratype">para-</typeStatus>
|
||
or poly- (
|
||
<bibRefCitation id="E91A4B5DDD38FFF514AAFAC6FA3C1715" author="Hennig W" box="[1269,1412,1332,1356]" pageId="6" pageNumber="322" refId="ref5240" refString="Hennig W (1966) ' Phylogenetic Systematics. ' (University of Illinois: Urbana, IL, USA)" type="book" year="1966">Hennig 1966</bibRefCitation>
|
||
).
|
||
</paragraph>
|
||
<paragraph id="8D3436ACDD38FFF5136DFA73FA2717C4" blockId="6.[818,1439,1409,1437]" box="[818,1439,1409,1437]" pageId="6" pageNumber="322">
|
||
<heading id="D67C81C0DD38FFF5136DFA73FA2717C4" bold="true" box="[818,1439,1409,1437]" fontSize="11" level="3" pageId="6" pageNumber="322" reason="9">
|
||
<emphasis id="BFFFEABEDD38FFF5136DFA73FA2717C4" bold="true" box="[818,1439,1409,1437]" pageId="6" pageNumber="322">Reproductive systems and gene concordance</emphasis>
|
||
</heading>
|
||
</paragraph>
|
||
<paragraph id="8D3436ACDD38FFF5136DFA46FC621512" blockId="6.[818,1490,1460,1932]" pageId="6" pageNumber="322">
|
||
To examine the effect of the reproductive strategy on the concordance between gene trees and the concatenated phylogeny,
|
||
<collectionCode id="EB9AAE69DD38FFF513D6FA07FC291455" box="[905,913,1525,1548]" country="Romania" lsid="urn:lsid:biocol.org:col:14415" name="&quot;Alexandru Ioan Cuza&quot; University" pageId="6" pageNumber="322" type="Herbarium">I</collectionCode>
|
||
calculated gCFs. My expectation was they would be higher in non-outcrossing
|
||
<taxonomicName id="4A8B4D2FDD38FFF51409F9E6FB401472" box="[1110,1272,1555,1579]" class="Magnoliopsida" family="Asteraceae" genus="Pogonolepis" kingdom="Plantae" order="Asterales" pageId="6" pageNumber="322" phylum="Tracheophyta" rank="species" species="muelleriana">
|
||
<emphasis id="BFFFEABEDD38FFF51409F9E6FB401472" bold="true" box="[1110,1272,1555,1579]" italics="true" pageId="6" pageNumber="322">P. muelleriana</emphasis>
|
||
</taxonomicName>
|
||
, because it seemed logical to assume that it would show less recombination between different genes than outcrossing
|
||
<taxonomicName id="4A8B4D2FDD38FFF514A1F9A6FAE11435" box="[1278,1369,1620,1644]" class="Magnoliopsida" family="Asteraceae" genus="Pogonolepis" kingdom="Plantae" order="Asterales" pageId="6" pageNumber="322" phylum="Tracheophyta" rank="species" species="strica">
|
||
<emphasis id="BFFFEABEDD38FFF514A1F9A6FAE11435" bold="true" box="[1278,1369,1620,1644]" italics="true" pageId="6" pageNumber="322">P. strica</emphasis>
|
||
</taxonomicName>
|
||
. However, with the exception of one outlier branch, the opposite was the case, and despite equal and geographically dispersed sampling, the median gCF was much higher in the outcrossing species. Randomisation of terminal names to simulate complete recombination between genes in one species produced gCF values in line with those observed in non-outcrossing
|
||
<taxonomicName id="4A8B4D2FDD38FFF5136DF8C6FC6B1512" box="[818,979,1843,1867]" class="Magnoliopsida" family="Asteraceae" genus="Pogonolepis" kingdom="Plantae" order="Asterales" pageId="6" pageNumber="322" phylum="Tracheophyta" rank="species" species="muelleriana">
|
||
<emphasis id="BFFFEABEDD38FFF5136DF8C6FC6B1512" bold="true" box="[818,979,1843,1867]" italics="true" pageId="6" pageNumber="322">P. muelleriana</emphasis>
|
||
</taxonomicName>
|
||
.
|
||
</paragraph>
|
||
<paragraph id="8D3436ACDD38FFF4130DF8A6FEF51312" blockId="6.[818,1490,1460,1932]" lastBlockId="7.[98,770,180,1931]" lastPageId="7" lastPageNumber="323" pageId="6" pageNumber="322">Values of sCF were close to 33% across internal nodes in both species, implying that their internal structure is poorly resolved in both cases, as would be expected if there is either no phylogenetic structure because of recombination or too few informative characters. The large number of informative characters in both species suggests that the former is more likely to be the case.</paragraph>
|
||
<paragraph id="8D3436ACDD39FFF410DDFEA6FEFE1175" blockId="7.[98,770,180,1931]" pageId="7" pageNumber="323">
|
||
It is nonetheless unclear why the outcrossing species shows unexpectedly high gene concordance and lower heterozygosity than does its non-outcrossing sister. To understand the processes behind the genetic structure of the species, a more detailed understanding of the reproductive system will be required.
|
||
<bibRefCitation id="E91A4B5DDD39FFF410AEFE06FEC71055" author="Short PS" box="[241,383,500,524]" pageId="7" pageNumber="323" pagination="237 - 253" refId="ref6179" refString="Short PS (1986) A revision of Pogonolepis (Compositae: Inuleae: Gnaphaliinae). Muelleria 6, 237 - 253. doi: 10.5962 / p. 184052" type="journal article" year="1986">Short (1986)</bibRefCitation>
|
||
interpreted
|
||
<taxonomicName id="4A8B4D2FDD39FFF41255FE06FD141052" box="[522,684,499,523]" class="Magnoliopsida" family="Asteraceae" genus="Pogonolepis" kingdom="Plantae" order="Asterales" pageId="7" pageNumber="323" phylum="Tracheophyta" rank="species" species="muelleriana">
|
||
<emphasis id="BFFFEABEDD39FFF41255FE06FD141052" bold="true" box="[522,684,499,523]" italics="true" pageId="7" pageNumber="323">P. muelleriana</emphasis>
|
||
</taxonomicName>
|
||
as selfing. Although he cautioned that the possibility of apomixis could not be excluded on available evidence, he associated it with abnormal pollen formation as found in
|
||
<taxonomicName id="4A8B4D2FDD39FFF412DBFDA6FEC510D5" authority="Weber (Richards 1973)" authorityName="Weber (Richards" authorityYear="1973" class="Magnoliopsida" family="Asteraceae" genus="Taraxacum" kingdom="Plantae" order="Asterales" pageId="7" pageNumber="323" phylum="Tracheophyta" rank="genus">
|
||
<emphasis id="BFFFEABEDD39FFF412DBFDA6FCB91032" bold="true" box="[644,769,596,619]" italics="true" pageId="7" pageNumber="323">Taraxacum</emphasis>
|
||
Weber (
|
||
<bibRefCitation id="E91A4B5DDD39FFF4109AFD86FECA10D5" author="Richards AJ" box="[197,370,628,652]" pageId="7" pageNumber="323" pagination="189 - 211" refId="ref5990" refString="Richards AJ (1973) The origin of Taraxacum agamospecies. Botanical Journal of the Linnean Society 66, 189 - 211. doi: 10.1111 / j. 1095 - 8339.1973. tb 02169. x" type="journal article" year="1973">Richards 1973</bibRefCitation>
|
||
)
|
||
</taxonomicName>
|
||
, which he did not observe in the species. However, there is no reason to assume that apomicts show abortive pollen, and pseudogamous apomicts require (potentially self-)pollination to activate the endosperm even as no fertilisation of the egg cell takes place (
|
||
<bibRefCitation id="E91A4B5DDD39FFF41034FCE6FE8E1175" author="Noirot M & Couvet D & Hamon S" box="[107,310,787,812]" pageId="7" pageNumber="323" pagination="479 - 483" refId="ref5905" refString="Noirot M, Couvet D, Hamon S (1997) Main role of self-pollination rate on reproductive allocations in pseudogamous apomicts. Theoretical and Applied Genetics 95, 479 - 483. doi: 10.1007 / s 001220050586" type="journal article" year="1997">
|
||
Noirot
|
||
<emphasis id="BFFFEABEDD39FFF410E3FCE6FF4B1172" bold="true" box="[188,243,787,811]" italics="true" pageId="7" pageNumber="323">et al.</emphasis>
|
||
1997
|
||
</bibRefCitation>
|
||
).
|
||
</paragraph>
|
||
<paragraph id="8D3436ACDD39FFF410DDFCC6FF741755" blockId="7.[98,770,180,1931]" pageId="7" pageNumber="323">
|
||
There are therefore several possibilities for the reproductive system of
|
||
<taxonomicName id="4A8B4D2FDD39FFF41152FCA6FE091135" box="[269,433,852,876]" class="Magnoliopsida" family="Asteraceae" genus="Pogonolepis" kingdom="Plantae" order="Asterales" pageId="7" pageNumber="323" phylum="Tracheophyta" rank="species" species="muelleriana">
|
||
<emphasis id="BFFFEABEDD39FFF41152FCA6FE091135" bold="true" box="[269,433,852,876]" italics="true" pageId="7" pageNumber="323">P. muelleriana</emphasis>
|
||
</taxonomicName>
|
||
, including selfing, facultative apomixis, and obligate apomixis. In the first two cases, outcrossing is still possible, albeit, presumably, much less likely to happen than in
|
||
<taxonomicName id="4A8B4D2FDD39FFF41161FC46FE1D1195" box="[318,421,948,972]" class="Magnoliopsida" family="Asteraceae" genus="Pogonolepis" kingdom="Plantae" order="Asterales" pageId="7" pageNumber="323" phylum="Tracheophyta" rank="species" species="stricta">
|
||
<emphasis id="BFFFEABEDD39FFF41161FC46FE1D1195" bold="true" box="[318,421,948,972]" italics="true" pageId="7" pageNumber="323">P. stricta</emphasis>
|
||
</taxonomicName>
|
||
, because of the much smaller number of pollen grains. It would be difficult to emasculate the minuscule, sequentially maturing flowers of a capitulum, but flow cytometric examination of fruits would allow selfing and apomixis to be differentiated by the genome-size ratio of embryo and endosperm (
|
||
<bibRefCitation id="E91A4B5DDD39FFF4112DFBA6FD891635" author="Matzk F & Meister A & Schubert I" box="[370,561,1108,1132]" pageId="7" pageNumber="323" pagination="97 - 108" refId="ref5508" refString="Matzk F, Meister A, Schubert I (2001) An efficient screen for reproductive pathways using mature seeds of monocots and dicots. The Plant Journal 21, 97 - 108. doi: 10.1046 / j. 1365 - 313 x. 2000.00647. x" type="journal article" year="2001">
|
||
Matzk
|
||
<emphasis id="BFFFEABEDD39FFF411E4FBA6FE491635" bold="true" box="[443,497,1108,1132]" italics="true" pageId="7" pageNumber="323">et al.</emphasis>
|
||
2001
|
||
</bibRefCitation>
|
||
;
|
||
<bibRefCitation id="E91A4B5DDD39FFF41261FBA6FD4A1635" author="Chen SH & Guja LK & Schmidt-Lebuhn AN" box="[574,754,1108,1132]" pageId="7" pageNumber="323" pagination="917 - 926" refId="ref5072" refString="Chen SH, Guja LK, Schmidt-Lebuhn AN (2019) Conservation implications of widespread polyploidy and apomixis: a case study in the genus Pomaderris (Rhamnaceae). Conservation Genetics 20, 917 - 926. doi: 10.1007 / s 10592 - 019 - 01184 - 2" type="journal article" year="2019">
|
||
Chen
|
||
<emphasis id="BFFFEABEDD39FFF41223FBA6FD0A1635" bold="true" box="[636,690,1108,1132]" italics="true" pageId="7" pageNumber="323">et al.</emphasis>
|
||
2019
|
||
</bibRefCitation>
|
||
). In addition to the reproductive system, dispersal distances would influence the genetic structure of a species (
|
||
<bibRefCitation id="E91A4B5DDD39FFF412FDFB66FE871695" author="Hamrick JL & Loveless MD" pageId="7" pageNumber="323" pagination="211 - 223" refId="ref5166" refString="Hamrick JL, Loveless MD (1986) The influence of seed dispersal mechanisms on the genetic structure of plant populations. In ' Frugivores Seed Dispersal. Tasks for Vegetation Science'. (Eds A Estrada, TH Fleming) Vol. 15, pp. 211 - 223. (Springer Netherlands: Dordrecht, Netherlands) doi: 10.1007 / 978 - 94 - 009 - 4812 - 9 _ 20" type="journal article" year="1986">Hamrick and Loveless 1986</bibRefCitation>
|
||
), but the two species of
|
||
<taxonomicName id="4A8B4D2FDD39FFF412DEFB41FCBA1692" box="[641,770,1203,1227]" class="Magnoliopsida" family="Asteraceae" genus="Pogonolepis" kingdom="Plantae" order="Asterales" pageId="7" pageNumber="323" phylum="Tracheophyta" rank="genus">
|
||
<emphasis id="BFFFEABEDD39FFF412DEFB41FCBA1692" bold="true" box="[641,770,1203,1227]" italics="true" pageId="7" pageNumber="323">Pogonolepis</emphasis>
|
||
</taxonomicName>
|
||
do not differ in their fruit morphology or adaptations to dispersal.
|
||
</paragraph>
|
||
<paragraph id="8D3436ACDD39FFF410DDFAE6FDED14F5" blockId="7.[98,770,180,1931]" pageId="7" pageNumber="323">
|
||
Another possibility is that an allopolyploid origin of at least part of
|
||
<taxonomicName id="4A8B4D2FDD39FFF4109BFAC6FE601715" box="[196,472,1332,1356]" class="Magnoliopsida" family="Asteraceae" genus="Pogonolepis" kingdom="Plantae" order="Asterales" pageId="7" pageNumber="323" phylum="Tracheophyta" rank="species" species="muelleriana">
|
||
<emphasis id="BFFFEABEDD39FFF4109BFAC6FE601715" bold="true" box="[196,472,1332,1356]" italics="true" pageId="7" pageNumber="323">Pogonolepis muelleriana</emphasis>
|
||
</taxonomicName>
|
||
explains the unexpected genetic structure, because the species is known to have either 2
|
||
<emphasis id="BFFFEABEDD39FFF4102FFA86FFC517D2" bold="true" box="[112,125,1396,1419]" italics="true" pageId="7" pageNumber="323">n</emphasis>
|
||
= 12 or ~20–24 chromosomes, compared with 2
|
||
<emphasis id="BFFFEABEDD39FFF412C5FA86FD1F17D2" bold="true" box="[666,679,1396,1419]" italics="true" pageId="7" pageNumber="323">n</emphasis>
|
||
=
|
||
<quantity id="4A739B49DD39FFF41294FA86FFC017F5" metricMagnitude="-1" metricUnit="m" metricValue="2.286" metricValueMax="2.54" metricValueMin="2.032" pageId="7" pageNumber="323" unit="in" value="9.0" valueMax="10.0" valueMin="8.0">8–10 in</quantity>
|
||
<taxonomicName id="4A8B4D2FDD39FFF410DDFA66FE3B17F5" authority="(Short 1986)" baseAuthorityName="Short" baseAuthorityYear="1986" box="[130,387,1428,1452]" class="Magnoliopsida" family="Asteraceae" genus="Pogonolepis" kingdom="Plantae" order="Asterales" pageId="7" pageNumber="323" phylum="Tracheophyta" rank="species" species="stricta">
|
||
<emphasis id="BFFFEABEDD39FFF410DDFA66FF5F17F5" bold="true" box="[130,231,1428,1452]" italics="true" pageId="7" pageNumber="323">P. stricta</emphasis>
|
||
(
|
||
<bibRefCitation id="E91A4B5DDD39FFF410A4FA66FEC017F5" author="Short PS" box="[251,376,1428,1452]" pageId="7" pageNumber="323" pagination="237 - 253" refId="ref6179" refString="Short PS (1986) A revision of Pogonolepis (Compositae: Inuleae: Gnaphaliinae). Muelleria 6, 237 - 253. doi: 10.5962 / p. 184052" type="journal article" year="1986">Short 1986</bibRefCitation>
|
||
)
|
||
</taxonomicName>
|
||
. In this scenario, alleles inherited from divergent parental populations would produce higher heterozygosity and potentially lower gene concordance than expected from a non-outcrossing diploid. HybPiper produced more paralog warnings for samples of
|
||
<taxonomicName id="4A8B4D2FDD39FFF4125EF9E6FD181472" box="[513,672,1555,1579]" class="Magnoliopsida" family="Asteraceae" genus="Pogonolepis" kingdom="Plantae" order="Asterales" pageId="7" pageNumber="323" phylum="Tracheophyta" rank="species" species="muelleriana">
|
||
<emphasis id="BFFFEABEDD39FFF4125EF9E6FD181472" bold="true" box="[513,672,1555,1579]" italics="true" pageId="7" pageNumber="323">P. muelleriana</emphasis>
|
||
</taxonomicName>
|
||
(median 23, mean 25.8) than for
|
||
<taxonomicName id="4A8B4D2FDD39FFF41124F9C6FE581415" baseAuthorityName="Short" baseAuthorityYear="1986" box="[379,480,1588,1612]" class="Magnoliopsida" family="Asteraceae" genus="Pogonolepis" kingdom="Plantae" order="Asterales" pageId="7" pageNumber="323" phylum="Tracheophyta" rank="species" species="stricta">
|
||
<emphasis id="BFFFEABEDD39FFF41124F9C6FE581415" bold="true" box="[379,480,1588,1612]" italics="true" pageId="7" pageNumber="323">P. stricta</emphasis>
|
||
</taxonomicName>
|
||
(median 15, mean 20.0), which could be seen to provide some support for this interpretation under the assumption that autopolyploidy would lead to paralogs too similar to be recognised.
|
||
</paragraph>
|
||
<paragraph id="8D3436ACDD39FFF410DDF946FF5315D2" blockId="7.[98,770,180,1931]" pageId="7" pageNumber="323">
|
||
What complicates this interpretation is that gene heterozygosity values in both species of>90% are unexpectedly high and in line with what would be expected of hybrids (
|
||
<bibRefCitation id="E91A4B5DDD39FFF41034F8E6FEC21575" author="Nauheimer L & Weigner N & Joyce E & Crayn D & Clarke C & Nargar K" box="[107,378,1811,1836]" pageId="7" pageNumber="323" pagination="11441" refId="ref5853" refString="Nauheimer L, Weigner N, Joyce E, Crayn D, Clarke C, Nargar K (2021) HybPhaser: a workflow for the detection and phasing of hybrids in target capture data sets. Applications in Plant Sciences 9, e 11441. doi: 10.1002 / aps 3.11441" type="journal article" year="2021">
|
||
Nauheimer
|
||
<emphasis id="BFFFEABEDD39FFF410AAF8E6FE8A1572" bold="true" box="[245,306,1811,1835]" italics="true" pageId="7" pageNumber="323">et al.</emphasis>
|
||
2021
|
||
</bibRefCitation>
|
||
). This raises the possibility that the entire genus may have a polyploidisation event in its recent ancestry, even before the duplication in
|
||
<emphasis id="BFFFEABEDD39FFF412D1F8A6FF5C15D2" bold="true" italics="true" pageId="7" pageNumber="323">
|
||
Pogonlepis
|
||
<taxonomicName id="4A8B4D2FDD39FFF4103DF881FF5C15D2" box="[98,228,1907,1931]" class="Magnoliopsida" family="Asteraceae" genus="Pogonolepis" kingdom="Plantae" order="Asterales" pageId="7" pageNumber="323" phylum="Tracheophyta" rank="species" species="muelleriana">muelleriana</taxonomicName>
|
||
</emphasis>
|
||
.
|
||
</paragraph>
|
||
<paragraph id="8D3436ACDD39FFF4130DFF46FB4E1055" blockId="7.[818,1490,180,524]" pageId="7" pageNumber="323">
|
||
Finally, the high heterozygosity itself suggests another possible explanation. The phylogeny was inferred using data assembled with HybPiper, which returns a single sequence for a locus if variants of that locus are not divergent enough to be flagged as possible paralogs. This means that different alleles of a heterozygous locus may be retrieved effectively randomly from each sample, and this could then reduce gene concordance on the phylogeny. Given that non-outcrossing
|
||
<taxonomicName id="4A8B4D2FDD39FFF4136DFE41FB841392" box="[818,1084,435,459]" class="Magnoliopsida" family="Asteraceae" genus="Pogonolepis" kingdom="Plantae" order="Asterales" pageId="7" pageNumber="323" phylum="Tracheophyta" rank="species" species="muelleriana">
|
||
<emphasis id="BFFFEABEDD39FFF4136DFE41FB841392" bold="true" box="[818,1084,435,459]" italics="true" pageId="7" pageNumber="323">Pogonolepis muelleriana</emphasis>
|
||
</taxonomicName>
|
||
showed higher gene heterozygosity, this effect may be more pronounced in that species, leading to its lower observed gene concordance.
|
||
</paragraph>
|
||
<paragraph id="8D3436ACDD39FFF4136EFDCEFA011001" blockId="7.[817,1465,572,600]" box="[817,1465,572,600]" pageId="7" pageNumber="323">
|
||
<heading id="D67C81C0DD39FFF4136EFDCEFA011001" bold="true" box="[817,1465,572,600]" fontSize="11" level="3" pageId="7" pageNumber="323" reason="9">
|
||
<emphasis id="BFFFEABEDD39FFF4136EFDCEFA011001" bold="true" box="[817,1465,572,600]" pageId="7" pageNumber="323">Utility of sequence capture at the species level</emphasis>
|
||
</heading>
|
||
</paragraph>
|
||
<paragraph id="8D3436ACDD39FFF4136DFD9DFAF41111" blockId="7.[818,1490,623,1415]" pageId="7" pageNumber="323">
|
||
The confirmation as being misidentified after morphological re-examination of all specimens seemingly in the ‘wrong’ position in the molecular phylogeny demonstrates the feasibility of diagnosing species affiliation using Angiosperms353 sequence capture data, assuming sufficient reference data are available, in this case for several specimens each of the two species of
|
||
<taxonomicName id="4A8B4D2FDD39FFF41387FCDDFBE1111E" box="[984,1113,815,839]" class="Magnoliopsida" family="Asteraceae" genus="Pogonolepis" kingdom="Plantae" order="Asterales" pageId="7" pageNumber="323" phylum="Tracheophyta" rank="genus">
|
||
<emphasis id="BFFFEABEDD39FFF41387FCDDFBE1111E" bold="true" box="[984,1113,815,839]" italics="true" pageId="7" pageNumber="323">Pogonolepis</emphasis>
|
||
</taxonomicName>
|
||
and some outgroups.
|
||
</paragraph>
|
||
<paragraph id="8D3436ACDD39FFF4130DFCA2FA3C1691" blockId="7.[818,1490,623,1415]" pageId="7" pageNumber="323">
|
||
In addition, the data resolved geographic structure for both species of
|
||
<taxonomicName id="4A8B4D2FDD39FFF413ADFC9DFBCB11DE" box="[1010,1139,879,903]" class="Magnoliopsida" family="Asteraceae" genus="Pogonolepis" kingdom="Plantae" order="Asterales" pageId="7" pageNumber="323" phylum="Tracheophyta" rank="genus">
|
||
<emphasis id="BFFFEABEDD39FFF413ADFC9DFBCB11DE" bold="true" box="[1010,1139,879,903]" italics="true" pageId="7" pageNumber="323">Pogonolepis</emphasis>
|
||
</taxonomicName>
|
||
. In
|
||
<taxonomicName id="4A8B4D2FDD39FFF414F3FC9DFAEA11DE" box="[1196,1362,879,903]" class="Magnoliopsida" family="Asteraceae" genus="Pogonolepis" kingdom="Plantae" order="Asterales" pageId="7" pageNumber="323" phylum="Tracheophyta" rank="species" species="muelleriana">
|
||
<emphasis id="BFFFEABEDD39FFF414F3FC9DFAEA11DE" bold="true" box="[1196,1362,879,903]" italics="true" pageId="7" pageNumber="323">P. muelleriana</emphasis>
|
||
</taxonomicName>
|
||
, the three Western Australian specimens formed a grade under the eastern specimens, as expected from a western origin of this species followed by dispersal to the east. Specimens of
|
||
<taxonomicName id="4A8B4D2FDD39FFF4136DFC1DFC2C165E" baseAuthorityName="Short" baseAuthorityYear="1986" box="[818,916,1007,1031]" class="Magnoliopsida" family="Asteraceae" genus="Pogonolepis" kingdom="Plantae" order="Asterales" pageId="7" pageNumber="323" phylum="Tracheophyta" rank="species" species="stricta">
|
||
<emphasis id="BFFFEABEDD39FFF4136DFC1DFC2C165E" bold="true" box="[818,916,1007,1031]" italics="true" pageId="7" pageNumber="323">P. stricta</emphasis>
|
||
</taxonomicName>
|
||
were split approximately evenly into two strongly supported clades, marked
|
||
<collectionCode id="EB9AAE69DD39FFF4143EFBFDFBCA167E" box="[1121,1138,1039,1063]" country="USA" lsid="urn:lsid:biocol.org:col:15406" name="Harvard University - Arnold Arboretum" pageId="7" pageNumber="323" type="Herbarium">A</collectionCode>
|
||
and
|
||
<collectionCode id="EB9AAE69DD39FFF414EFFBE2FB07167E" box="[1200,1215,1040,1063]" country="Germany" lsid="urn:lsid:biocol.org:col:15534" name="Botanischer Garten und Botanisches Museum Berlin-Dahlem, Zentraleinrichtung der Freien Universitaet" pageId="7" pageNumber="323" type="Herbarium">B</collectionCode>
|
||
in
|
||
<figureCitation id="15B02A29DD39FFF414B5FBFDFA94167E" box="[1258,1324,1039,1063]" captionStart="Fig" captionStartId="5.[175,212,1745,1768]" captionTargetBox="[140,1429,163,1709]" captionTargetId="figure-2@5.[183,1391,185,1703]" captionTargetPageId="5" captionText="Fig. 3. Likelihood phylogeny of concatenated supermatrix of Pogonolepis and outgroups. Numbers above branches indicate UltraFast Bootstrap values, gene Concordance Factors, and site Concordance Factors. The dashed branch was shortened for the figure. Western Australian specimens of P. muelleriana are marked with (WA), all others are from eastern states. A and B indicate informally named clades inside P. stricta." figureDoi="http://doi.org/10.5281/zenodo.10974469" httpUri="https://zenodo.org/record/10974469/files/figure.png" pageId="7" pageNumber="323">Fig. 3</figureCitation>
|
||
. Clade
|
||
<collectionCode id="EB9AAE69DD39FFF415D9FBFDFA2F167E" box="[1414,1431,1039,1063]" country="USA" lsid="urn:lsid:biocol.org:col:15406" name="Harvard University - Arnold Arboretum" pageId="7" pageNumber="323" type="Herbarium">A</collectionCode>
|
||
contained samples from south-western
|
||
<collectingRegion id="4F4FF84EDD39FFF41491FBC2FA21161E" box="[1230,1433,1071,1095]" country="Australia" name="Western Australia" pageId="7" pageNumber="323">Western Australia</collectingRegion>
|
||
in a triangle bounded by just south of Shark Bay, Bunbury, and Hyden. Clade
|
||
<collectionCode id="EB9AAE69DD39FFF4138EFB82FC5816DE" box="[977,992,1136,1159]" country="Germany" lsid="urn:lsid:biocol.org:col:15534" name="Botanischer Garten und Botanisches Museum Berlin-Dahlem, Zentraleinrichtung der Freien Universitaet" pageId="7" pageNumber="323" type="Herbarium">B</collectionCode>
|
||
comprised samples from a northern, mostly interior, area approximately bounded by just east of Shark Bay, Geraldton, the Hamersley Lakes and Lake Way.
|
||
</paragraph>
|
||
<paragraph id="8D3436ACDD39FFF4130DFB3DFC2117DE" blockId="7.[818,1490,623,1415]" pageId="7" pageNumber="323">That this level of resolution can be achieved inside species suggests sequence capture as an attractive approach for phylogeographic studies, not least because it is able to produce data more reliably from herbarium specimens with potentially degraded DNA than are many other molecular methods.</paragraph>
|
||
</subSubSection>
|
||
</treatment>
|
||
</document> |