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<document id="5E3273E0DEF80504801D11C39722F8D8" ID-DOI="10.3897/mycokeys.105.119536" ID-publisher-id="119536" URI-arpha="54724331-66A7-5219-95B5-7306653A0A2D" XM.bibliography_approvedBy="admin" XM.materialsCitations_approvedBy="admin" XM.taxonomicNames_approvedBy="admin" article-type="research-article" checkinTime="1717010140543" checkinUser="pensoft" docAuthor="Wang, Yuchun, Tu, Yiyi, Chen, Xueling, Jiang, Hong, Ren, Hengze, Lu, Qinhua, Wei, Chaoling &amp; Lv, Wuyun" docDate="2024" docId="049807CF8E1459BCA2B63DE0622DDA56" docLanguage="en" docName="MycoKeys 105: 217-251" docOrigin="MycoKeys 105" docSource="https://mycokeys.pensoft.net/article/119536/download/xml/" docStyle="DocumentStyle:PensoftTaxPub.0000.journal_article.mycokeys" docStyleName="PensoftTaxPub.0000.journal_article.mycokeys" docTitle="Epicoccum puerense Y. Wang, Y. Tu, X. Chen, H. Jiang, H. Ren, Q. Lu, C. Wei &amp; W. Lv 2024, sp. nov." docType="treatment" docVersion="3" dtd-version="3.0" lastPageNumber="251" masterDocId="5472433166A7521995B57306653A0A2D" masterDocTitle="Didymellaceae species associated with tea plant (Camellia sinensis) in China" masterLastPageNumber="251" masterPageNumber="217" pageNumber="217" updateTime="1717029935025" updateUser="admin">
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<mods:title id="8A8CC1750667347331EA63584A210061">Didymellaceae species associated with tea plant (Camellia sinensis) in China</mods:title>
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<mods:name id="EA25321099C79B91AC387FB9F85DB8B1" type="personal">
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<mods:namePart id="BE77FB53B13D622469B085347A91F394">Wang, Yuchun</mods:namePart>
<mods:affiliation id="A3BB708DE61361907F98FB51B7F07665">College of Tea Science and Tea Culture, Zhejiang A &amp; F University, Hangzhou 311300, Zhejiang, China</mods:affiliation>
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<mods:namePart id="DB654479D8C6BE7979656D6D2AE95A30">Tu, Yiyi</mods:namePart>
<mods:affiliation id="048AC54EC60FE58D63F70B05D455BE5D">College of Tea Science and Tea Culture, Zhejiang A &amp; F University, Hangzhou 311300, Zhejiang, China</mods:affiliation>
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<mods:namePart id="9B15D3DC5249E55723286B6B4D051C93">Chen, Xueling</mods:namePart>
<mods:affiliation id="8BAB02D99EC21D012633C08982BA01BD">College of Tea Science and Tea Culture, Zhejiang A &amp; F University, Hangzhou 311300, Zhejiang, China</mods:affiliation>
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<mods:namePart id="979083609F18917313A1897AEE9DD208">Jiang, Hong</mods:namePart>
<mods:affiliation id="70DCFAA13BBAC883F60CB58EFB43642D">College of Tea Science and Tea Culture, Zhejiang A &amp; F University, Hangzhou 311300, Zhejiang, China</mods:affiliation>
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<mods:namePart id="77F7F3A808582CB6FF26153CB8746847">Ren, Hengze</mods:namePart>
<mods:affiliation id="847736AB7057D14EBC32CF881A71612C">College of Tea Science and Tea Culture, Zhejiang A &amp; F University, Hangzhou 311300, Zhejiang, China</mods:affiliation>
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<mods:namePart id="293E8FB3C164223569BA4F58B7C111BA">Lu, Qinhua</mods:namePart>
<mods:affiliation id="3C91ABD3697E15145B16282E3638FC08">Institute of Sericulture and Tea, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China</mods:affiliation>
</mods:name>
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<mods:namePart id="54D964BE51BC50CCEE82EC4ADA39301B">Wei, Chaoling</mods:namePart>
<mods:affiliation id="054E9614AFA06C694E1585E691146459">State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, 130 Changjiang West Road, Hefei, 230036, Anhui, China</mods:affiliation>
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<mods:namePart id="0414F5727B0D5EE8FD6DC2F0E6423A96">Lv, Wuyun</mods:namePart>
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<mods:affiliation id="F1194E96FD83BED2CF97D472F35F9699">College of Tea Science and Tea Culture, Zhejiang A &amp; F University, Hangzhou 311300, Zhejiang, China</mods:affiliation>
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<mods:date id="CC932E5811FD8CA62561B2A2E36EDAE2">2024</mods:date>
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<mods:number id="1FA571FA019658EDCB9B9755B8C7F2E4">105</mods:number>
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<mods:identifier id="6DDF0C9464DCB37A7293996A41A8C4C6" type="DOI">10.3897/mycokeys.105.119536</mods:identifier>
</mods:mods>
<treatment id="049807CF8E1459BCA2B63DE0622DDA56" ID-DOI="http://doi.org/10.5281/zenodo.11391015" ID-Zenodo-Dep="11391015" ID-arpha="049807CF-8E14-59BC-A2B6-3DE0622DDA56" ID-mycobank="848999" LSID="urn:lsid:plazi:treatment:049807CF8E1459BCA2B63DE0622DDA56" httpUri="http://treatment.plazi.org/id/049807CF8E1459BCA2B63DE0622DDA56">
<subSubSection id="7BEB079C5B76045CF3DCF031E1564D8D" type="nomenclature">
<paragraph id="05AEBE92181DF6982DE0ADE66B231EB2">
<taxonomicName id="E9AF624E42FAE23129539EC21FA61806" authority="Y. Wang, Y. Tu, X. Chen, H. Jiang, H. Ren, Q. Lu, C. Wei &amp; W. Lv" authorityName="Y. Wang, Y. Tu, X. Chen, H. Jiang, H. Ren, Q. Lu, C. Wei &amp; W. Lv" authorityYear="2024" class="Dothideomycetes" family="Didymellaceae" genus="Epicoccum" kingdom="Fungi" order="Pleosporales" phylum="Ascomycota" rank="species" species="puerense" status="sp. nov.">
<emphasis id="B0F3EA874F27A32A111FAD4AD58744F9" italics="true">Epicoccum puerense</emphasis>
Y. Wang, Y. Tu, X. Chen, H. Jiang, H. Ren, Q. Lu, C. Wei &amp; W. Lv
</taxonomicName>
<taxonomicNameLabel id="B79D1D610E54A1DE57813D525184E92A" rank="species">sp. nov.</taxonomicNameLabel>
</paragraph>
</subSubSection>
<subSubSection id="FBEC1C4801A348B93CDAA849D62ADC57" type="description">
<paragraph id="BDEB367A32DF3E419321611819FBC027">
<figureCitation id="507F5668323EFF648BC4C11A0FA026E3" captionStart="Figure 8" captionStartId="F8" captionText="Figure 8. Epicoccum puerense YCW 2117 A, B colony on PDA (front and reverse) C, D colony on OA (front and reverse) E mycelia F pycnidia forming on PDA G conidia. Scale bars: 10 μm (E G)." figureDoi="10.3897/mycokeys.105.119536.figure8" httpUri="https://binary.pensoft.net/fig/1058576">Fig. 8</figureCitation>
</paragraph>
</subSubSection>
<subSubSection id="SECID0EZZBI" type="etymology">
<paragraph id="A9C1CF53F14D3030E0C32488CA8EEE43">
<heading id="22B59CB29C32F68DE7968525BD11A0AC" reason="title">Etymology.</heading>
</paragraph>
<paragraph id="6AEC3A4A82669A73CF465487875079F6">Named after the location where it was collected, Puer City.</paragraph>
</subSubSection>
<subSubSection id="SECID0E5ZBI" type="description">
<paragraph id="714CACBACF52303DE162D2C3BF413584">
<heading id="1EE889348ED33B6A1CCC480EFC161085" reason="title">Description.</heading>
</paragraph>
<paragraph id="148D17E03DB23B29FC200E02FF0742A1">
<emphasis id="CA7AD227D19615B220F20F108D129678" bold="true" italics="true">Sexual morph</emphasis>
: undetermined.
<emphasis id="8293FB221B6F9B3FDE432455D158047B" bold="true" italics="true">Asexual morph</emphasis>
: Pycnidia smooth, subglobose to ellipsoidal, hyaline. Conidia were not of uniform size, ellipsoidal to subcylindrical, pale yellow to green, smooth- and thin-walled, abundant, generated from pycnidia, aseptate, 6.815 × 3.67.2 µm (av. = 9.7 ± 1.9 × 4.7 ± 0.7 µm, n = 30). Mycelia lateral branching, septate, hyaline.
</paragraph>
</subSubSection>
<subSubSection id="SECID0EM1BI" type="description">
<paragraph id="117139AFAD8EFFC0165AD7AD47C9A0CA">
<heading id="67442D7ABB2340017CDC31C0518EBE63" reason="title">Culture characteristics.</heading>
</paragraph>
<paragraph id="D4BE1726F45817A086BA00D453038989">
Colonies on PDA reaching
<quantity id="09B10508F8BE977B8C55C5FC1F9117B1" metricMagnitude="-2" metricUnit="m" metricValue="3.65" metricValueMax="4.1" metricValueMin="3.2" unit="mm" value="36.5" valueMax="41.0" valueMin="32.0">3241 mm</quantity>
diam. after 7 days at 28 ° C in the dark, margin irregular, aerial mycelium flat, olivaceous to buff, white edges; reverse black to brown, pale buff edges. Pycnidia and conidia produced on the colony surface after cultured for 14 days at 28 ° C in the dark. Colonies on
<abbrev id="ABBRID0ES1BI" xlink_title="oatmeal agar">OA</abbrev>
reaching
<quantity id="FDE471256CD4E7B6FEAAE8D02318A2AF" metricMagnitude="-2" metricUnit="m" metricValue="5.45" metricValueMax="5.8" metricValueMin="5.1" unit="mm" value="54.5" valueMax="58.0" valueMin="51.0">5158 mm</quantity>
diam. after 7 days at 28 ° C in the dark, margin regular, aerial mycelium flat, rosy to pale green, white edges; reverse pale buff to whitish.
</paragraph>
<caption id="0AFD789C10A000C826803C033391FD09" ID-DOI="10.3897/mycokeys.105.119536.figure8" ID-arpha="2C359AD1-6E9A-59A2-B9BB-FE4F2F1B9157" httpUri="https://binary.pensoft.net/fig/1058576" startId="F8">
<paragraph id="77A9C6B6CAD88E5F35B1C4C870EBB5AB">
<label id="245AFF1F5270542825782E0DAC7B16DA">Figure 8.</label>
</paragraph>
<paragraph id="E9D76996E87D387EF732EBFEA5AF3D9D">
<taxonomicName id="6642BAB9A9A455E01F3A943FB22B2E51" authority="YCW 2117" authorityName="YCW" authorityYear="2117" class="Dothideomycetes" family="Didymellaceae" genus="Epicoccum" kingdom="Fungi" order="Pleosporales" phylum="Ascomycota" rank="species" species="puerense">
<emphasis id="EC3C099CD65E50494E8CD5AF75860029" italics="true">Epicoccum puerense</emphasis>
<specimenCode id="744825DA34780B790FE546C26CE89D1C">YCW 2117</specimenCode>
</taxonomicName>
<emphasis id="C5A96C19312601DCAE060A4E049E8656" bold="true">A, B</emphasis>
colony on PDA (front and reverse)
<emphasis id="F3032470246382A1F2331F39FCCC23E2" bold="true">C, D</emphasis>
colony on
<abbrev id="ABBRID0EN2BI" xlink_title="oatmeal agar">OA</abbrev>
(front and reverse)
<emphasis id="A5B6727ACB2ABD1636E8644424FCC209" bold="true">E</emphasis>
mycelia
<emphasis id="C0980A48A47E96F82F84F286B9ADB5C6" bold="true">F</emphasis>
pycnidia forming on PDA
<emphasis id="32DDC2B14DC58D4C9414945C083B68CB" bold="true">G</emphasis>
conidia. Scale bars: 10 μm (
<emphasis id="F0D1DA796D165E5DBA961C00A8BF1070" bold="true">E G</emphasis>
).
</paragraph>
</caption>
</subSubSection>
<subSubSection id="SECID0E52BI" type="material">
<paragraph id="8BB585E9DE0E92118A8E875359D2AA2F">
<heading id="1A04EA1277AB0EC7D9518F029AD4DE48" reason="title">Materials examined.</heading>
</paragraph>
<paragraph id="871328425E3D09DAF29D86A5AA8947CA">
<materialsCitation id="4247D4DB14F351C0851424277EE9B651" collectedFrom="from diseased leaves of C. sinensis" collectingDate="2020-06-13" collectionCode="HMAS, CGMCC, YCW" collectorName="Y. C. Wang" country="China" county="Jingdong Yizu Autonomous County" location="Puer City" municipality="Puer City" specimenCode="HMAS 352390, CGMCC 3.24249, YCW 2117" specimenCount="1" stateProvince="Yunnan Province" typeStatus="holotype">
<collectingCountry id="B2D9C21BFCFD7E8B58DB2A2BFDFC13D4" name="China">China</collectingCountry>
,
<collectingRegion id="4808E5F329D4CF672EBE5C4EECD8E09F" country="China" name="Yunnan">Yunnan Province</collectingRegion>
,
<collectingMunicipality id="9E378CACDC8ABEB151EAAC24D158DC8B">Puer City</collectingMunicipality>
,
<collectingCounty id="3C34E7954D0B722E77F253B1D083BBCA">Jingdong Yizu Autonomous County</collectingCounty>
,
<collectedFrom id="71018E5920D55C3F786C4B319515FAAC">
from diseased leaves of
<taxonomicName id="871903D0C360B18A557EFA34B6842FB2" authorityName="YCW" authorityYear="1884" class="Magnoliopsida" family="Theaceae" genus="Camellia" kingdom="Plantae" order="Ericales" phylum="Tracheophyta" rank="species" species="sinensis">
<emphasis id="972110223421C4D9AAF36DECC6F80F1D" italics="true">C. sinensis</emphasis>
</taxonomicName>
</collectedFrom>
,
<date id="88F6084693118362F0905DD5DAC6CBCA" value="2020-06-13">
<collectingDate id="6734C932743770037CD268AFE6204AC1" value="2020-06-13">13 Jun 2020</collectingDate>
</date>
,
<collectorName id="5339AD9C000FE20EBEFF634E2E6AD7F9">Y. C. Wang</collectorName>
,
<typeStatus id="0EDEC10E8B5CBDFDD933D3B6CE516E6A" type="holotype">Holotype</typeStatus>
<specimenCode id="DFB29F119B0084470C2F59B3BD913FD0">
<named-content id="9FAEB722BFA0094E783497CDD8F87FEF" content-type="dwc:institutional_code" xlink_href="http://grbio.org/institution/chinese-academy-sciences" xlink_title="Mycological Herbarium, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China">HMAS</named-content>
352390
</specimenCode>
, culture ex-type
<specimenCode id="498CB21D85216FC6E12DF5E32F8F7034">
<named-content id="674D53A6C2B1008FBB2DC16F7ED708CF" content-type="dwc:institutional_code" xlink_href="http://grbio.org/institution/china-general-microbiological-culture-collection-center-chinese-academy-sciences" xlink_title="China General Microbiological Culture Collection Center">CGMCC</named-content>
3.24249
</specimenCode>
=
<specimenCode id="20C9F6ABB1B51DF3B36413B5587F728B">YCW 2117</specimenCode>
</materialsCitation>
.
<materialsCitation id="34F41299E4546F13F4EC6D6B4AFDBD14" collectedFrom="from healthy leaves of C. sinensis cv. Dalicha" collectingDate="2019-06-22" collectionCode="CGMCC, YCW" collectorName="Y. C. Wang" location="Yunnan Province" specimenCode="CGMCC 3.24243, YCW 224" specimenCount="1" stateProvince="Yunnan Province">
<collectingRegion id="FAADEBA7A6B3228775B60FE87D720427" country="China" name="Yunnan">Yunnan Province</collectingRegion>
,
<collectedFrom id="5B897B434C955EA16AA3CE1FCD9D9F8A">
from healthy leaves of
<taxonomicName id="F71278866448FB170FA5736F6E78B305" authorityName="YCW" authorityYear="1884" class="Magnoliopsida" family="Theaceae" genus="Camellia" kingdom="Plantae" order="Ericales" phylum="Tracheophyta" rank="species" species="sinensis">
<emphasis id="7ACA75F88F034F546EDAA52ADA14A527" italics="true">C. sinensis</emphasis>
</taxonomicName>
cv.
<emphasis id="2B2058DE0938D46601784F24730F63C3" italics="true">Dalicha</emphasis>
</collectedFrom>
,
<date id="87F676208B4C293A236360DE14CB0E9B" value="2019-06-22">
<collectingDate id="1BFA04D2B078DDC913DC421E5CE0A9AB" value="2019-06-22">22 Jun 2019</collectingDate>
</date>
,
<collectorName id="8CE03B232F0FABCDA65104167F7CA39C">Y. C. Wang</collectorName>
, culture ex-type
<specimenCode id="93467653F09D0897E5AB390A7B6ED571">
<named-content id="E0B474D6044CCD5B6BE9D73B6DF3937E" content-type="dwc:institutional_code" xlink_href="http://grbio.org/institution/china-general-microbiological-culture-collection-center-chinese-academy-sciences" xlink_title="China General Microbiological Culture Collection Center">CGMCC</named-content>
3.24243
</specimenCode>
=
<specimenCode id="9AABF0476D630512F7A78E8583DF85CD">YCW 224</specimenCode>
</materialsCitation>
.
</paragraph>
</subSubSection>
<subSubSection id="SECID0EQ4BI" type="notes">
<paragraph id="C289137CAF167E9AB1569CD36CC2C1D4">
<heading id="2B3E7D21084E1FC9E8FBB183DC1DC81A" reason="title">Notes.</heading>
</paragraph>
<paragraph id="9E0B019D8D907A6B0C8BD35AA67D2179">
<taxonomicName id="85F36CBE1A83A069655A64D54AA8E667" authorityName="YCW" authorityYear="2117" class="Dothideomycetes" family="Didymellaceae" genus="Epicoccum" kingdom="Fungi" order="Pleosporales" phylum="Ascomycota" rank="species" species="puerense">
<emphasis id="6443EA36F80EC4DD9AF9907D28D1CEC6" italics="true">Epicoccum puerense</emphasis>
</taxonomicName>
is closely related to
<taxonomicName id="487419727E5E61ACD8659CE10B99B081" class="Dothideomycetes" family="Didymellaceae" genus="Epicoccum" kingdom="Fungi" order="Pleosporales" phylum="Ascomycota" rank="species" species="dendrobii">
<emphasis id="E4E61A577416D6321FA5F4A302A8BAD2" italics="true">E. dendrobii</emphasis>
</taxonomicName>
with high statistical support (Fig.
<figureCitation id="8146ACCB82F14C814D559148EF1BF0B6" captionStart="Figure 3" captionStartId="F3" captionText="Figure 3. Phylogenetic tree generated by Maximum Likelihood analysis, based on the combined ITS, LSU, RPB 2 and TUB 2 dataset of Epicoccum species. Bootstrap support values above 50 % and Bayesian posterior values above 0.75 are shown at each node (ML / PP). Pleiochaeta setosa CBS 118.25 / CBS 496.63 and Co. palmarum CBS 400.71 are used as outgroups. Ex-type strains are emphasised in bold." figureDoi="10.3897/mycokeys.105.119536.figure3" httpUri="https://binary.pensoft.net/fig/1058571">3</figureCitation>
).
<taxonomicName id="2BCBEAD11567A9EE92BAD02AF3E2E85A" authorityName="YCW" authorityYear="2117" class="Dothideomycetes" family="Didymellaceae" genus="Epicoccum" kingdom="Fungi" order="Pleosporales" phylum="Ascomycota" rank="species" species="puerense">
<emphasis id="823A47356C970DF6BCE5FC665ABE720C" italics="true">Epicoccum puerense</emphasis>
</taxonomicName>
has 1 bp difference in ITS from
<taxonomicName id="0B40FFFFBB0478A01AE17F46164E18F8" class="Dothideomycetes" family="Didymellaceae" genus="Epicoccum" kingdom="Fungi" order="Pleosporales" phylum="Ascomycota" rank="species" species="dendrobii">
<emphasis id="B09672770D0C37CDCD77E9616CEA2367" italics="true">E. dendrobii</emphasis>
</taxonomicName>
. The conidia size is larger than that of
<taxonomicName id="CD46F6DABCD7EC3CA1264DAEA08247C5" class="Dothideomycetes" family="Didymellaceae" genus="Epicoccum" kingdom="Fungi" order="Pleosporales" phylum="Ascomycota" rank="species" species="dendrobii">
<emphasis id="FD0C86EF1B825E5E859D10C35310D4B6" italics="true">E. dendrobii</emphasis>
</taxonomicName>
. In the present study, five strains were isolated from healthy or diseased tea plant leaves. It was isolated and identified from tea plant for the first time.
</paragraph>
</subSubSection>
</treatment>
</document>