243 lines
37 KiB
XML
243 lines
37 KiB
XML
<document id="2B7108F4C88B9FA5A7DE6219F75EF18E" ID-DOI="10.11646/phytotaxa.630.3.4" ID-ISSN="1179-3163" ID-Zenodo-Dep="10410012" IM.bibliography_approvedBy="guilherme" IM.illustrations_approvedBy="felipe" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.tables_requiresApprovalFor="GgImagineBatch" IM.taxonomicNames_approvedBy="guilherme" IM.treatments_approvedBy="felipe" checkinTime="1703073505141" checkinUser="plazi" docAuthor="Wang, Xin-Ju, Li, Neng-Qi & Gao, Jiang-Yun" docDate="2023" docId="03C087AFDB50FF89FF16FCA6D0E28352" docLanguage="en" docName="phytotaxa.630.3.4.pdf" docOrigin="Phytotaxa 630 (3)" docSource="https://phytotaxa.mapress.com/pt/article/download/phytotaxa.630.3.4/51378" docStyle="DocumentStyle:96748F8F1B6C902996E134952A3A36B9.13:Phytotaxa.2014-.journal_article" docStyleId="96748F8F1B6C902996E134952A3A36B9" docStyleName="Phytotaxa.2014-.journal_article" docStyleVersion="13" docTitle="Serendipita officinale X. J. Wang 2023, sp. nov." docType="treatment" docVersion="3" lastPageNumber="231" masterDocId="FFF9FFD7DB52FF8BFF9EFFD6D3378B58" masterDocTitle="Serendipita officinale sp. nov. (Serendipitaceae): a new species of orchid mycorrhizal fungus" masterLastPageNumber="240" masterPageNumber="229" pageNumber="231" updateTime="1704195529969" updateUser="guilherme" zenodo-license-figures="UNSPECIFIED" zenodo-license-treatments="UNSPECIFIED">
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<mods:title id="8BC9450DDF4759EC71217AA4EAA9806F">Serendipita officinale sp. nov. (Serendipitaceae): a new species of orchid mycorrhizal fungus</mods:title>
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<mods:name id="2AFC5BFBEEC223CB860A3DA78CD79809" type="personal">
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<mods:roleTerm id="87EB013BD6E014D97930538F63674889">Author</mods:roleTerm>
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<mods:namePart id="3C6E8350E2EDFAB5445CE51716A3A80F">Wang, Xin-Ju</mods:namePart>
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<mods:nameIdentifier id="59E7E28A72D42A5719AE63FA92AEE1FA" type="ORCID">0000-0001-5337-9614</mods:nameIdentifier>
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<mods:affiliation id="EA482660263E1178A52F35A530939F5D">Institute of Biodiversity, School of Ecology and Environmental Science, Yunnan University, Kunming, Yunnan 650500, China.</mods:affiliation>
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<mods:nameIdentifier id="372CABAFF1B1EBC54FE6E3BCF4D42C29" type="email">1247849502@qq.com</mods:nameIdentifier>
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<mods:roleTerm id="20F1852282E91D597F8BEA1474552066">Author</mods:roleTerm>
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<mods:namePart id="D4C13A6227C17C7987870A9238E1200C">Li, Neng-Qi</mods:namePart>
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<mods:nameIdentifier id="DBED54F34A5AAF39EF234CA36BC98D51" type="ORCID">0009-0000-7751-0509</mods:nameIdentifier>
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<mods:affiliation id="C45B149E1241711F9807698B8F2E09C8">College of Landscape Architecture and Horticulture Sciences, Southwest Forestry University, Kunming, Yunnan 650224, China.</mods:affiliation>
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<mods:nameIdentifier id="959077752B78BE27F9306F7DE95A47A7" type="email">632514709@qq.com</mods:nameIdentifier>
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<mods:roleTerm id="47245B257CAD31D32916AEC43C3E4700">Author</mods:roleTerm>
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<mods:namePart id="AC1BEBC3A630FCED542E1E99DAF4AB7D">Gao, Jiang-Yun</mods:namePart>
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<mods:nameIdentifier id="47FA6B14FC9BBA1ADDE477D852522192" type="ORCID">0000-0003-1541-5922</mods:nameIdentifier>
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<mods:affiliation id="1B4B4C32AC3D60810A4123971B2E93AC">Institute of Biodiversity, School of Ecology and Environmental Science, Yunnan University, Kunming, Yunnan 650500, China.</mods:affiliation>
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<mods:nameIdentifier id="ACF0338B86DC484F8FB5E44C627DEC15" type="email">jiangyun.gao@ynu.edu.cn</mods:nameIdentifier>
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<mods:title id="3AB2CE9F74C7064A6775FF20BA725CDB">Phytotaxa</mods:title>
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<mods:part id="D1CF29DC7BDF785C9F009749DD3FF3F9">
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<mods:date id="78ACF6C5978187736B191F0FC22B200D">2023</mods:date>
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<mods:number id="5604751DFA433CFC5102C324AAAF83D7">2023-12-20</mods:number>
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<mods:detail id="81DC841511EDE5D4FAD8486B16EAD6CC" type="volume">
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<mods:number id="EF154491372D36D9961C933FBE39719B">630</mods:number>
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<mods:url id="0FD79010E17BA901D3F5C865AAA1D43E">https://phytotaxa.mapress.com/pt/article/download/phytotaxa.630.3.4/51378</mods:url>
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<mods:classification id="A9F5C92D5E0A110BAD9B4FB1BF7F5F9E">journal article</mods:classification>
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<mods:identifier id="4ADF511C53B9E770DCF1DDA884FE987D" type="DOI">10.11646/phytotaxa.630.3.4</mods:identifier>
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<mods:identifier id="69983117E07D7E4EC7FD40126C223211" type="ISSN">1179-3163</mods:identifier>
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<mods:identifier id="E003872D38F69E97B320CB43EBF1F753" type="Zenodo-Dep">10410012</mods:identifier>
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</mods:mods>
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<treatment id="03C087AFDB50FF89FF16FCA6D0E28352" LSID="urn:lsid:plazi:treatment:03C087AFDB50FF89FF16FCA6D0E28352" httpUri="http://treatment.plazi.org/id/03C087AFDB50FF89FF16FCA6D0E28352" lastPageNumber="231" pageId="2" pageNumber="231">
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<subSubSection id="C3736532DB50FF89FF16FCA6D25288F5" pageId="2" pageNumber="231" type="nomenclature">
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<paragraph id="8BD636B9DB50FF89FF16FCA6D1F488D2" blockId="2.[136,707,880,907]" box="[136,707,880,907]" pageId="2" pageNumber="231">
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<heading id="D09E81D5DB50FF89FF16FCA6D1F488D2" box="[136,707,880,907]" fontSize="11" level="2" pageId="2" pageNumber="231" reason="3">
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<taxonomicName id="4C694D3ADB50FF89FF16FCA6D13088D3" authority="X. J. Wang" authorityName="X. J. Wang" authorityYear="2023" box="[136,519,880,907]" class="Agaricomycetes" family="Serendipitaceae" genus="Serendipita" kingdom="Fungi" order="Auriculariales" pageId="2" pageNumber="231" phylum="Basidiomycota" rank="species" species="officinale" status="sp. nov.">
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<emphasis id="B91DEAABDB50FF89FF16FCA6D2B688D3" bold="true" box="[136,385,880,907]" italics="true" pageId="2" pageNumber="231">Serendipita officinale</emphasis>
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||
X. J. Wang
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||
</taxonomicName>
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||
,
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||
<taxonomicNameLabel id="A22E57D0DB50FF89FD8FFCA7D15F88D3" box="[529,616,881,907]" pageId="2" pageNumber="231" rank="species">
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<emphasis id="B91DEAABDB50FF89FD8FFCA7D15788D2" box="[529,608,881,906]" italics="true" pageId="2" pageNumber="231">sp. nov</emphasis>
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||
.
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||
</taxonomicNameLabel>
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||
(
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||
<figureCitation id="13522A3CDB50FF89FDE9FCA6D18088D2" box="[631,695,880,906]" captionStart="FIGURE 1" captionStartId="3.[136,229,993,1015]" captionTargetBox="[152,1430,177,964]" captionTargetId="figure-20@3.[151,1436,171,966]" captionTargetPageId="3" captionText="FIGURE 1. Morphological features of Serendipita officinalesp. nov. a, b: Colonies on PDA after 14 and 30 days; c: aerial mycelium; d: Transmission electron micrograph showing monilioid hyphae with a septate (arrows); e: DAPI-stained hyphae (n = nuclei; S = septa); f & g: Micrograph and scanning electron micrograph of hyphae on the agar surface showing typical hyphal coils; h: Branched monilioid cell chains under microscope; i: Monilioid hyphae under scanning electron micrograph; j: Scanning electron micrograph showing chlamydospores in the roots of Dendrobium officinale; k: Micrograph showing chlamydospores in the roots of Dendrobium flexicaule. l: Transmission electron micrograph of the dolipore septum with a flat and imperforate parenthesome (arrow) as typical for members of the Sebacinales." figureDoi="http://doi.org/10.5281/zenodo.10410014" httpUri="https://zenodo.org/record/10410014/files/figure.png" pageId="2" pageNumber="231">Fig. 1</figureCitation>
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||
)
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||
</heading>
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||
</paragraph>
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||
<paragraph id="8BD636B9DB50FF89FF16FC4ED25288F5" blockId="2.[136,357,920,942]" box="[136,357,920,942]" pageId="2" pageNumber="231">MycoBank MB809112</paragraph>
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||
</subSubSection>
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||
<subSubSection id="C3736532DB50FF89FF16FC0AD00A8F42" pageId="2" pageNumber="231" type="diagnosis">
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<paragraph id="8BD636B9DB50FF89FF16FC0AD00A8F42" blockId="2.[136,1452,988,2059]" pageId="2" pageNumber="231">
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||
<emphasis id="B91DEAABDB50FF89FF16FC0AD23588AE" bold="true" box="[136,258,988,1014]" pageId="2" pageNumber="231">Diagnosis.</emphasis>
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Differs from other
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<taxonomicName id="4C694D3ADB50FF89FE47FC0AD18788AE" box="[473,688,988,1014]" pageId="2" pageNumber="231">
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<emphasis id="B91DEAABDB50FF89FE47FC0AD16E88AE" box="[473,601,988,1014]" italics="true" pageId="2" pageNumber="231">Serendipita</emphasis>
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species
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</taxonomicName>
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by abundant villiform and felty aerial mycelium, uninucleate hyphal cells, and the highest growth rate in terms of colony diameter.
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</paragraph>
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||
</subSubSection>
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||
<subSubSection id="C3736532DB50FF89FF23FBF2D12B8FDE" pageId="2" pageNumber="231" type="materials_examined">
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<paragraph id="8BD636B9DB50FF89FF23FBF2D12B8FDE" blockId="2.[136,1452,988,2059]" pageId="2" pageNumber="231">
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||
<emphasis id="B91DEAABDB50FF89FF23FBF2D3C88F66" bold="true" box="[189,255,1060,1086]" pageId="2" pageNumber="231">Type.</emphasis>
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||
<materialsCitation id="3B013CE4DB50FF89FE98FBF2D12F8FDE" collectingDate="2018-09" collectionCode="CCTCC" country="China" elevation="1200" latitude="30.516666" location="Luotian town" longLatPrecision="1221" longitude="108.55" municipality="Wanzhou District" pageId="2" pageNumber="231" specimenCode="HMAS 352471, M 2019744, MN173026" specimenCount="1" stateProvince="Chongqing" typeStatus="holotype">
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||
<collectingCountry id="F37E7629DB50FF89FE98FBF2D27A8F66" box="[262,333,1060,1086]" name="China" pageId="2" pageNumber="231">China</collectingCountry>
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||
.
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||
<collectingRegion id="49ADF85BDB50FF89FEC9FBF2D1748F66" box="[343,579,1060,1086]" country="China" name="Chongqing" pageId="2" pageNumber="231">Chongqing Province</collectingRegion>
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||
:
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||
<collectingMunicipality id="6BB2ACC3DB50FF89FDD3FBF2D0238F66" box="[589,788,1060,1086]" pageId="2" pageNumber="231">Wanzhou District</collectingMunicipality>
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||
,
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||
<location id="8EB66062DB50FF89FC81FBF2D08F8F67" LSID="urn:lsid:plazi:treatment:03C087AFDB50FF89FF16FCA6D0E28352:8EB66062DB50FF89FC81FBF2D08F8F67" box="[799,952,1060,1087]" country="China" latitude="30.516666" longLatPrecision="1221" longitude="108.55" municipality="Wanzhou District" name="Luotian town" pageId="2" pageNumber="231" stateProvince="Chongqing">Luotian town</location>
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||
, isolated from protocorms and adult roots of
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<taxonomicName id="4C694D3ADB50FF89FF16FB9ED2B38F3A" box="[136,388,1096,1122]" class="Liliopsida" family="Orchidaceae" genus="Dendrobium" kingdom="Plantae" order="Asparagales" pageId="2" pageNumber="231" phylum="Tracheophyta" rank="species" species="officinale">
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<emphasis id="B91DEAABDB50FF89FF16FB9ED2B38F3A" box="[136,388,1096,1122]" italics="true" pageId="2" pageNumber="231">Dendrobium officinale</emphasis>
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</taxonomicName>
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,
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||
<geoCoordinate id="EE5D507EDB50FF89FE0FFB9FD2C58F3C" box="[401,498,1097,1124]" degrees="30" direction="north" minutes="31" orientation="latitude" pageId="2" pageNumber="231" precision="925" value="30.516666">30°31′N</geoCoordinate>
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,
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<geoCoordinate id="EE5D507EDB50FF89FE68FB9FD1568F3C" box="[502,609,1097,1124]" degrees="108" direction="east" minutes="33" orientation="longitude" pageId="2" pageNumber="231" precision="925" value="108.55">108°33′E</geoCoordinate>
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||
,
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<quantity id="4C919B5CDB50FF89FDF5FB9ED1F68F3B" box="[619,705,1096,1123]" metricMagnitude="3" metricUnit="m" metricValue="1.2" pageId="2" pageNumber="231" unit="m" value="1200.0">
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<elevation id="0044D18ADB50FF89FDF5FB9ED1F68F3B" box="[619,705,1096,1123]" metricMagnitude="3" metricUnit="m" metricValue="1.2" pageId="2" pageNumber="231" unit="m" value="1200.0">1200 m</elevation>
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</quantity>
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, alt.,
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<date id="FFD71079DB50FF89FD62FB9ED0458F3A" box="[764,882,1096,1122]" pageId="2" pageNumber="231" value="2018-09">
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||
<collectingDate id="EF93E991DB50FF89FD62FB9ED0458F3A" box="[764,882,1096,1122]" pageId="2" pageNumber="231" value="2018-09">Sept. 2018</collectingDate>
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</date>
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||
(
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<typeStatus id="54D2881BDB50FF89FC1EFB9ED0D38F3A" box="[896,996,1096,1122]" pageId="2" pageNumber="231" type="holotype">holotype</typeStatus>
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<specimenCode id="DBCF9EC2DB50FF89FC77FB9ED7AF8F3B" box="[1001,1176,1096,1123]" collectionCode="HMAS" country="China" httpUri="http://grbio.org/cool/qht3-g0qs" name="Chinese Academy of Sciences" pageId="2" pageNumber="231" type="Herbarium">HMAS 352471</specimenCode>
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||
; ex-type culture
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<collectionCode id="ED78AE7CDB50FF89FAD1FB9ED69C8F3A" box="[1359,1451,1096,1122]" country="China" httpUri="http://grbio.org/cool/bzd1-vhw1" name="China Center for Type Culture Collection" pageId="2" pageNumber="231">CCTCC</collectionCode>
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||
<specimenCode id="DBCF9EC2DB50FF89FF16FBBBD2398FDE" box="[136,270,1132,1159]" collectionCode="M" country="Germany" lsid="urn:lsid:biocol.org:col:15637" name="Botanische Staatssammlung München" pageId="2" pageNumber="231" type="Herbarium">M 2019744</specimenCode>
|
||
; GenBank
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||
<specimenCode id="DBCF9EC2DB50FF89FE14FBBAD1278FDE" box="[394,528,1132,1158]" collectionCode="MN" country="Brazil" name="Museu Nacional, Universidade Federal do Rio de Janeiro" pageId="2" pageNumber="231">MN173026</specimenCode>
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||
)
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||
</materialsCitation>
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||
.
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||
</paragraph>
|
||
</subSubSection>
|
||
<subSubSection id="C3736532DB50FF89FF23FB46D2848F96" pageId="2" pageNumber="231" type="etymology">
|
||
<paragraph id="8BD636B9DB50FF89FF23FB46D2848F96" blockId="2.[136,1452,988,2059]" pageId="2" pageNumber="231">
|
||
<emphasis id="B91DEAABDB50FF89FF23FB46D2738FF2" bold="true" box="[189,324,1168,1194]" pageId="2" pageNumber="231">Etymology.</emphasis>
|
||
<taxonomicName id="4C694D3ADB50FF89FED0FB46D28F8FF2" box="[334,440,1168,1194]" class="Liliopsida" family="Orchidaceae" genus="Dendrobium" kingdom="Plantae" order="Asparagales" pageId="2" pageNumber="231" phylum="Tracheophyta" rank="species" species="officinale">
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||
<emphasis id="B91DEAABDB50FF89FED0FB46D28F8FF2" box="[334,440,1168,1194]" italics="true" pageId="2" pageNumber="231">officinale</emphasis>
|
||
</taxonomicName>
|
||
, referring to the plant species,
|
||
<taxonomicName id="4C694D3ADB50FF89FCBCFB47D0878FF2" box="[802,944,1168,1194]" class="Liliopsida" family="Orchidaceae" genus="Dendrobium" kingdom="Plantae" order="Asparagales" pageId="2" pageNumber="231" phylum="Tracheophyta" rank="species" species="officinale">
|
||
<emphasis id="B91DEAABDB50FF89FCBCFB47D0878FF2" box="[802,944,1168,1194]" italics="true" pageId="2" pageNumber="231">D. officinale</emphasis>
|
||
</taxonomicName>
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||
from whose protocorms and adult roots the species was first collected.
|
||
</paragraph>
|
||
</subSubSection>
|
||
<subSubSection id="C3736532DB50FF89FF23FB0ED66D8C9A" pageId="2" pageNumber="231" type="description">
|
||
<paragraph id="8BD636B9DB50FF89FF23FB0ED66D8C9A" blockId="2.[136,1452,988,2059]" pageId="2" pageNumber="231">
|
||
<emphasis id="B91DEAABDB50FF89FF23FB0ED2788FAA" bold="true" box="[189,335,1240,1266]" pageId="2" pageNumber="231">Description.</emphasis>
|
||
The fungal colony reaching
|
||
<quantity id="4C919B5CDB50FF89FD0CFB0ED1D68FAB" box="[658,737,1240,1267]" metricMagnitude="-2" metricUnit="m" metricValue="7.8" pageId="2" pageNumber="231" unit="mm" value="78.0">78 mm</quantity>
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||
in diam. on PDA medium at 25 °C in the dark after two weeks. The cultures are a cream to pale yellow in color with a circular or nearly circular growth habit; formed a rough, appressed, waxy, thick and moist layer with radial streaks near the edge; submerged mycelium was observed (
|
||
<figureCitation id="13522A3CDB50FF89FAD3FAF6D6AB8E62" box="[1357,1436,1312,1338]" captionStart="FIGURE 1" captionStartId="3.[136,229,993,1015]" captionTargetBox="[152,1430,177,964]" captionTargetId="figure-20@3.[151,1436,171,966]" captionTargetPageId="3" captionText="FIGURE 1. Morphological features of Serendipita officinalesp. nov. a, b: Colonies on PDA after 14 and 30 days; c: aerial mycelium; d: Transmission electron micrograph showing monilioid hyphae with a septate (arrows); e: DAPI-stained hyphae (n = nuclei; S = septa); f & g: Micrograph and scanning electron micrograph of hyphae on the agar surface showing typical hyphal coils; h: Branched monilioid cell chains under microscope; i: Monilioid hyphae under scanning electron micrograph; j: Scanning electron micrograph showing chlamydospores in the roots of Dendrobium officinale; k: Micrograph showing chlamydospores in the roots of Dendrobium flexicaule. l: Transmission electron micrograph of the dolipore septum with a flat and imperforate parenthesome (arrow) as typical for members of the Sebacinales." figureDoi="http://doi.org/10.5281/zenodo.10410014" httpUri="https://zenodo.org/record/10410014/files/figure.png" pageId="2" pageNumber="231">Fig. 1a</figureCitation>
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). After one month, abundant villiform and felty aerial mycelium in white could be observed in intermediate zone (
|
||
<figureCitation id="13522A3CDB50FF89FAE0FA92D3928EDA" captionStart="FIGURE 1" captionStartId="3.[136,229,993,1015]" captionTargetBox="[152,1430,177,964]" captionTargetId="figure-20@3.[151,1436,171,966]" captionTargetPageId="3" captionText="FIGURE 1. Morphological features of Serendipita officinalesp. nov. a, b: Colonies on PDA after 14 and 30 days; c: aerial mycelium; d: Transmission electron micrograph showing monilioid hyphae with a septate (arrows); e: DAPI-stained hyphae (n = nuclei; S = septa); f & g: Micrograph and scanning electron micrograph of hyphae on the agar surface showing typical hyphal coils; h: Branched monilioid cell chains under microscope; i: Monilioid hyphae under scanning electron micrograph; j: Scanning electron micrograph showing chlamydospores in the roots of Dendrobium officinale; k: Micrograph showing chlamydospores in the roots of Dendrobium flexicaule. l: Transmission electron micrograph of the dolipore septum with a flat and imperforate parenthesome (arrow) as typical for members of the Sebacinales." figureDoi="http://doi.org/10.5281/zenodo.10410014" httpUri="https://zenodo.org/record/10410014/files/figure.png" pageId="2" pageNumber="231">Fig. 1b</figureCitation>
|
||
), the emergence of aerial hyphae is clearly visible, and over time, their growth has become luxuriant (
|
||
<figureCitation id="13522A3CDB50FF89FA84FABED65D8EDA" box="[1306,1386,1384,1410]" captionStart="FIGURE 1" captionStartId="3.[136,229,993,1015]" captionTargetBox="[152,1430,177,964]" captionTargetId="figure-20@3.[151,1436,171,966]" captionTargetPageId="3" captionText="FIGURE 1. Morphological features of Serendipita officinalesp. nov. a, b: Colonies on PDA after 14 and 30 days; c: aerial mycelium; d: Transmission electron micrograph showing monilioid hyphae with a septate (arrows); e: DAPI-stained hyphae (n = nuclei; S = septa); f & g: Micrograph and scanning electron micrograph of hyphae on the agar surface showing typical hyphal coils; h: Branched monilioid cell chains under microscope; i: Monilioid hyphae under scanning electron micrograph; j: Scanning electron micrograph showing chlamydospores in the roots of Dendrobium officinale; k: Micrograph showing chlamydospores in the roots of Dendrobium flexicaule. l: Transmission electron micrograph of the dolipore septum with a flat and imperforate parenthesome (arrow) as typical for members of the Sebacinales." figureDoi="http://doi.org/10.5281/zenodo.10410014" httpUri="https://zenodo.org/record/10410014/files/figure.png" pageId="2" pageNumber="231">Fig. 1c</figureCitation>
|
||
). The hyphae were hyaline, ranging from 0.4 to 1.03 μm (0.58 ± 0.02 μm, n = 35). Hyphae elongated into arborization and often produced hyphal coils (
|
||
<figureCitation id="13522A3CDB50FF89FE4EFA66D1668E92" box="[464,593,1456,1482]" captionStart="FIGURE 1" captionStartId="3.[136,229,993,1015]" captionTargetBox="[152,1430,177,964]" captionTargetId="figure-20@3.[151,1436,171,966]" captionTargetPageId="3" captionText="FIGURE 1. Morphological features of Serendipita officinalesp. nov. a, b: Colonies on PDA after 14 and 30 days; c: aerial mycelium; d: Transmission electron micrograph showing monilioid hyphae with a septate (arrows); e: DAPI-stained hyphae (n = nuclei; S = septa); f & g: Micrograph and scanning electron micrograph of hyphae on the agar surface showing typical hyphal coils; h: Branched monilioid cell chains under microscope; i: Monilioid hyphae under scanning electron micrograph; j: Scanning electron micrograph showing chlamydospores in the roots of Dendrobium officinale; k: Micrograph showing chlamydospores in the roots of Dendrobium flexicaule. l: Transmission electron micrograph of the dolipore septum with a flat and imperforate parenthesome (arrow) as typical for members of the Sebacinales." figureDoi="http://doi.org/10.5281/zenodo.10410014" httpUri="https://zenodo.org/record/10410014/files/figure.png" pageId="2" pageNumber="231">Fig. 1f & g</figureCitation>
|
||
). As the hyphae aged, septate structures became evident (
|
||
<figureCitation id="13522A3CDB50FF89FB49FA66D61F8E92" box="[1239,1320,1456,1482]" captionStart="FIGURE 1" captionStartId="3.[136,229,993,1015]" captionTargetBox="[152,1430,177,964]" captionTargetId="figure-20@3.[151,1436,171,966]" captionTargetPageId="3" captionText="FIGURE 1. Morphological features of Serendipita officinalesp. nov. a, b: Colonies on PDA after 14 and 30 days; c: aerial mycelium; d: Transmission electron micrograph showing monilioid hyphae with a septate (arrows); e: DAPI-stained hyphae (n = nuclei; S = septa); f & g: Micrograph and scanning electron micrograph of hyphae on the agar surface showing typical hyphal coils; h: Branched monilioid cell chains under microscope; i: Monilioid hyphae under scanning electron micrograph; j: Scanning electron micrograph showing chlamydospores in the roots of Dendrobium officinale; k: Micrograph showing chlamydospores in the roots of Dendrobium flexicaule. l: Transmission electron micrograph of the dolipore septum with a flat and imperforate parenthesome (arrow) as typical for members of the Sebacinales." figureDoi="http://doi.org/10.5281/zenodo.10410014" httpUri="https://zenodo.org/record/10410014/files/figure.png" pageId="2" pageNumber="231">Fig. 1d</figureCitation>
|
||
), with each cell regularly containing 1 nucleus (
|
||
<figureCitation id="13522A3CDB50FF89FD84FA02D15E8EB6" box="[538,617,1492,1518]" captionStart="FIGURE 1" captionStartId="3.[136,229,993,1015]" captionTargetBox="[152,1430,177,964]" captionTargetId="figure-20@3.[151,1436,171,966]" captionTargetPageId="3" captionText="FIGURE 1. Morphological features of Serendipita officinalesp. nov. a, b: Colonies on PDA after 14 and 30 days; c: aerial mycelium; d: Transmission electron micrograph showing monilioid hyphae with a septate (arrows); e: DAPI-stained hyphae (n = nuclei; S = septa); f & g: Micrograph and scanning electron micrograph of hyphae on the agar surface showing typical hyphal coils; h: Branched monilioid cell chains under microscope; i: Monilioid hyphae under scanning electron micrograph; j: Scanning electron micrograph showing chlamydospores in the roots of Dendrobium officinale; k: Micrograph showing chlamydospores in the roots of Dendrobium flexicaule. l: Transmission electron micrograph of the dolipore septum with a flat and imperforate parenthesome (arrow) as typical for members of the Sebacinales." figureDoi="http://doi.org/10.5281/zenodo.10410014" httpUri="https://zenodo.org/record/10410014/files/figure.png" pageId="2" pageNumber="231">Fig. 1e</figureCitation>
|
||
), but clamp connections were absent. After about four weeks of culture on PDA, the fungus produced abundant monilioid hyphae arranged in chains of more than five cells (
|
||
<figureCitation id="13522A3CDB50FF89FB48FA2ED6638D4A" box="[1238,1364,1528,1554]" captionStart="FIGURE 1" captionStartId="3.[136,229,993,1015]" captionTargetBox="[152,1430,177,964]" captionTargetId="figure-20@3.[151,1436,171,966]" captionTargetPageId="3" captionText="FIGURE 1. Morphological features of Serendipita officinalesp. nov. a, b: Colonies on PDA after 14 and 30 days; c: aerial mycelium; d: Transmission electron micrograph showing monilioid hyphae with a septate (arrows); e: DAPI-stained hyphae (n = nuclei; S = septa); f & g: Micrograph and scanning electron micrograph of hyphae on the agar surface showing typical hyphal coils; h: Branched monilioid cell chains under microscope; i: Monilioid hyphae under scanning electron micrograph; j: Scanning electron micrograph showing chlamydospores in the roots of Dendrobium officinale; k: Micrograph showing chlamydospores in the roots of Dendrobium flexicaule. l: Transmission electron micrograph of the dolipore septum with a flat and imperforate parenthesome (arrow) as typical for members of the Sebacinales." figureDoi="http://doi.org/10.5281/zenodo.10410014" httpUri="https://zenodo.org/record/10410014/files/figure.png" pageId="2" pageNumber="231">Fig. 1h & i</figureCitation>
|
||
). In the inoculation treatments, the fungal strain was examined on their capacity to support seedling growth in
|
||
<taxonomicName id="4C694D3ADB50FF89FA81F9CAD3C58D02" class="Liliopsida" family="Orchidaceae" genus="Dendrobium" kingdom="Plantae" order="Asparagales" pageId="2" pageNumber="231" phylum="Tracheophyta" rank="species" species="officinale">
|
||
<emphasis id="B91DEAABDB50FF89FA81F9CAD3C58D02" italics="true" pageId="2" pageNumber="231">Dendrobium officinale</emphasis>
|
||
</taxonomicName>
|
||
and
|
||
<taxonomicName id="4C694D3ADB50FF89FEB8F997D2828D02" box="[294,437,1600,1626]" class="Liliopsida" family="Orchidaceae" genus="Dendrobium" kingdom="Plantae" order="Asparagales" pageId="2" pageNumber="231" phylum="Tracheophyta" rank="species" species="flexicaule">
|
||
<emphasis id="B91DEAABDB50FF89FEB8F997D2828D02" box="[294,437,1600,1626]" italics="true" pageId="2" pageNumber="231">D. flexicaule</emphasis>
|
||
</taxonomicName>
|
||
. Approximately six months after inoculation,
|
||
<taxonomicName id="4C694D3ADB50FF89FC2DF996D7938D02" authorityName="X. J. Wang" authorityYear="2023" box="[947,1188,1600,1626]" class="Agaricomycetes" genus="Serendipita" kingdom="Fungi" order="Auriculariales" pageId="2" pageNumber="231" phylum="Basidiomycota" rank="species" species="officinale">
|
||
<emphasis id="B91DEAABDB50FF89FC2DF996D7938D02" box="[947,1188,1600,1626]" italics="true" pageId="2" pageNumber="231">Serendipita officinale</emphasis>
|
||
</taxonomicName>
|
||
showed highly positive effects on seedling growth among all treatments in
|
||
<taxonomicName id="4C694D3ADB50FF89FD52F9B3D06B8D26" box="[716,860,1636,1662]" class="Liliopsida" family="Orchidaceae" genus="Dendrobium" kingdom="Plantae" order="Asparagales" pageId="2" pageNumber="231" phylum="Tracheophyta" rank="species" species="flexicaule">
|
||
<emphasis id="B91DEAABDB50FF89FD52F9B3D06B8D26" box="[716,860,1636,1662]" italics="true" pageId="2" pageNumber="231">D. flexicaule</emphasis>
|
||
</taxonomicName>
|
||
. In the
|
||
<taxonomicName id="4C694D3ADB50FF89FC2AF9B2D7928D26" authorityName="X. J. Wang" authorityYear="2023" box="[948,1189,1636,1662]" class="Agaricomycetes" genus="Serendipita" kingdom="Fungi" order="Auriculariales" pageId="2" pageNumber="231" phylum="Basidiomycota" rank="species" species="officinale">
|
||
<emphasis id="B91DEAABDB50FF89FC2AF9B2D7928D26" box="[948,1189,1636,1662]" italics="true" pageId="2" pageNumber="231">Serendipita officinale</emphasis>
|
||
</taxonomicName>
|
||
treatment, plant height (11.78 ±
|
||
<quantity id="4C919B5CDB50FF89FF74F95ED2778DFA" box="[234,320,1672,1698]" metricMagnitude="-4" metricUnit="m" metricValue="6.0" pageId="2" pageNumber="231" unit="mm" value="0.6">0.6 mm</quantity>
|
||
) and stem diameter (2.44 ±
|
||
<quantity id="4C919B5CDB50FF89FDEAF95ED1E08DFA" box="[628,727,1672,1698]" metricMagnitude="-4" metricUnit="m" metricValue="1.3" pageId="2" pageNumber="231" unit="mm" value="0.13">0.13 mm</quantity>
|
||
) were significantly higher than in the asymbiotic treatment (plant height: 9.58 ±
|
||
<quantity id="4C919B5CDB50FF89FEB5F97AD2A58D9F" box="[299,402,1708,1735]" metricMagnitude="-4" metricUnit="m" metricValue="3.5" pageId="2" pageNumber="231" unit="mm" value="0.35">0.35 mm</quantity>
|
||
; stem diameter: 1.94 ±
|
||
<quantity id="4C919B5CDB50FF89FD06F97AD0378D9E" box="[664,768,1708,1734]" metricMagnitude="-5" metricUnit="m" metricValue="9.0" pageId="2" pageNumber="231" unit="mm" value="0.09">0.09 mm</quantity>
|
||
), as well as in other inoculated treatments which plant height ranged from
|
||
<quantity id="4C919B5CDB50FF89FE88F906D2D28DB3" box="[278,485,1744,1771]" metricMagnitude="-2" metricUnit="m" metricValue="1.097" metricValueMax="1.103" metricValueMin="1.091" pageId="2" pageNumber="231" unit="mm" value="10.969999999999999" valueMax="11.03" valueMin="10.91">10.91 to 11.03 mm</quantity>
|
||
and stem diameter ranged from
|
||
<quantity id="4C919B5CDB50FF89FCD6F906D0CA8DB3" box="[840,1021,1744,1771]" metricMagnitude="-3" metricUnit="m" metricValue="2.165" metricValueMax="2.32" metricValueMin="2.01" pageId="2" pageNumber="231" unit="mm" value="2.165" valueMax="2.32" valueMin="2.01">2.01 to 2.32 mm</quantity>
|
||
(all P <0.05) (Wang
|
||
<emphasis id="B91DEAABDB50FF89FB78F907D62A8DB2" box="[1254,1309,1744,1770]" italics="true" pageId="2" pageNumber="231">et al.</emphasis>
|
||
unpublished data). For
|
||
<taxonomicName id="4C694D3ADB50FF89FE9DF923D2A48C56" authorityName="X. J. Wang" authorityYear="2023" box="[259,403,1780,1806]" class="Agaricomycetes" genus="Serendipita" kingdom="Fungi" order="Auriculariales" pageId="2" pageNumber="231" phylum="Basidiomycota" rank="species" species="officinale">
|
||
<emphasis id="B91DEAABDB50FF89FE9DF923D2A48C56" box="[259,403,1780,1806]" italics="true" pageId="2" pageNumber="231">D. officinale</emphasis>
|
||
</taxonomicName>
|
||
, after 90 days, most seedlings developed 3-5 leaves with strong roots, the percentages of seedlings (75.8 ± 2.6%) significantly higher than the percentages of the other treatments (P<0.001) (
|
||
<bibRefCitation id="EFF84B48DB50FF89FB7AF8CED6978C6A" author="Yang, H. & Li, N. Q. & Gao, J. Y." box="[1252,1440,1816,1842]" pageId="2" pageNumber="231" pagination="1114105" refId="ref7416" refString="Yang, H., Li, N. Q. & Gao, J. Y. (2023) A novel method to produce massive seedlings via symbiotic seed germination in orchids. Frontiers in Plant Science 14: 1114105. https: // doi. org / 10.3389 / fpls. 2023.1114105" type="journal article" year="2023">
|
||
Yang
|
||
<emphasis id="B91DEAABDB50FF89FAB8F8CFD66A8C6A" box="[1318,1373,1816,1842]" italics="true" pageId="2" pageNumber="231">et al.</emphasis>
|
||
2023
|
||
</bibRefCitation>
|
||
). After six months, chlamydospores (mature monilioid cells) were observed in the roots of both
|
||
<taxonomicName id="4C694D3ADB50FF89FB30F8EAD69B8C0E" box="[1198,1452,1852,1878]" class="Liliopsida" family="Orchidaceae" genus="Dendrobium" kingdom="Plantae" order="Asparagales" pageId="2" pageNumber="231" phylum="Tracheophyta" rank="species" species="officinale">
|
||
<emphasis id="B91DEAABDB50FF89FB30F8EAD69B8C0E" box="[1198,1452,1852,1878]" italics="true" pageId="2" pageNumber="231">Dendrobium officinale</emphasis>
|
||
</taxonomicName>
|
||
(
|
||
<figureCitation id="13522A3CDB50FF89FF0EF8B6D3ED8C22" box="[144,218,1888,1914]" captionStart="FIGURE 1" captionStartId="3.[136,229,993,1015]" captionTargetBox="[152,1430,177,964]" captionTargetId="figure-20@3.[151,1436,171,966]" captionTargetPageId="3" captionText="FIGURE 1. Morphological features of Serendipita officinalesp. nov. a, b: Colonies on PDA after 14 and 30 days; c: aerial mycelium; d: Transmission electron micrograph showing monilioid hyphae with a septate (arrows); e: DAPI-stained hyphae (n = nuclei; S = septa); f & g: Micrograph and scanning electron micrograph of hyphae on the agar surface showing typical hyphal coils; h: Branched monilioid cell chains under microscope; i: Monilioid hyphae under scanning electron micrograph; j: Scanning electron micrograph showing chlamydospores in the roots of Dendrobium officinale; k: Micrograph showing chlamydospores in the roots of Dendrobium flexicaule. l: Transmission electron micrograph of the dolipore septum with a flat and imperforate parenthesome (arrow) as typical for members of the Sebacinales." figureDoi="http://doi.org/10.5281/zenodo.10410014" httpUri="https://zenodo.org/record/10410014/files/figure.png" pageId="2" pageNumber="231">Fig. 1j</figureCitation>
|
||
) and
|
||
<taxonomicName id="4C694D3ADB50FF89FE85F8B7D29C8C22" box="[283,427,1888,1914]" class="Liliopsida" family="Orchidaceae" genus="Dendrobium" kingdom="Plantae" order="Asparagales" pageId="2" pageNumber="231" phylum="Tracheophyta" rank="species" species="flexicaule">
|
||
<emphasis id="B91DEAABDB50FF89FE85F8B7D29C8C22" box="[283,427,1888,1914]" italics="true" pageId="2" pageNumber="231">D. flexicaule</emphasis>
|
||
</taxonomicName>
|
||
(
|
||
<figureCitation id="13522A3CDB50FF89FE24F8B6D13A8C22" box="[442,525,1888,1914]" captionStart="FIGURE 1" captionStartId="3.[136,229,993,1015]" captionTargetBox="[152,1430,177,964]" captionTargetId="figure-20@3.[151,1436,171,966]" captionTargetPageId="3" captionText="FIGURE 1. Morphological features of Serendipita officinalesp. nov. a, b: Colonies on PDA after 14 and 30 days; c: aerial mycelium; d: Transmission electron micrograph showing monilioid hyphae with a septate (arrows); e: DAPI-stained hyphae (n = nuclei; S = septa); f & g: Micrograph and scanning electron micrograph of hyphae on the agar surface showing typical hyphal coils; h: Branched monilioid cell chains under microscope; i: Monilioid hyphae under scanning electron micrograph; j: Scanning electron micrograph showing chlamydospores in the roots of Dendrobium officinale; k: Micrograph showing chlamydospores in the roots of Dendrobium flexicaule. l: Transmission electron micrograph of the dolipore septum with a flat and imperforate parenthesome (arrow) as typical for members of the Sebacinales." figureDoi="http://doi.org/10.5281/zenodo.10410014" httpUri="https://zenodo.org/record/10410014/files/figure.png" pageId="2" pageNumber="231">Fig. 1k</figureCitation>
|
||
), which are globose, measuring 3–6 μm in diam, or sub-globose, measuring 3–7 × 2–5 μm. Neither conidiophores nor sexual structures were observed. Ultrastructure analysis revealed that the hyphae contained typical dolipore septa with imperforate parenthesomes (
|
||
<figureCitation id="13522A3CDB50FF89FCF2F87ED0818C9A" box="[876,950,1960,1986]" captionStart="FIGURE 1" captionStartId="3.[136,229,993,1015]" captionTargetBox="[152,1430,177,964]" captionTargetId="figure-20@3.[151,1436,171,966]" captionTargetPageId="3" captionText="FIGURE 1. Morphological features of Serendipita officinalesp. nov. a, b: Colonies on PDA after 14 and 30 days; c: aerial mycelium; d: Transmission electron micrograph showing monilioid hyphae with a septate (arrows); e: DAPI-stained hyphae (n = nuclei; S = septa); f & g: Micrograph and scanning electron micrograph of hyphae on the agar surface showing typical hyphal coils; h: Branched monilioid cell chains under microscope; i: Monilioid hyphae under scanning electron micrograph; j: Scanning electron micrograph showing chlamydospores in the roots of Dendrobium officinale; k: Micrograph showing chlamydospores in the roots of Dendrobium flexicaule. l: Transmission electron micrograph of the dolipore septum with a flat and imperforate parenthesome (arrow) as typical for members of the Sebacinales." figureDoi="http://doi.org/10.5281/zenodo.10410014" httpUri="https://zenodo.org/record/10410014/files/figure.png" pageId="2" pageNumber="231">Fig. 1l</figureCitation>
|
||
), as the characteristic in
|
||
<taxonomicName id="4C694D3ADB50FF89FB50F87ED6618C9A" box="[1230,1366,1960,1986]" class="Agaricomycetes" higherTaxonomySource="CoL,GBIF" kingdom="Fungi" order="Sebacinales" pageId="2" pageNumber="231" phylum="Basidiomycota" rank="order">Sebacinales</taxonomicName>
|
||
.
|
||
</paragraph>
|
||
</subSubSection>
|
||
<subSubSection id="C3736532DB50FF89FF23F81AD0E28352" pageId="2" pageNumber="231" type="biology_ecology">
|
||
<paragraph id="8BD636B9DB50FF89FF23F81AD0E28352" blockId="2.[136,1452,988,2059]" pageId="2" pageNumber="231">
|
||
<emphasis id="B91DEAABDB50FF89FF23F81AD2D88CBE" bold="true" box="[189,495,1996,2022]" pageId="2" pageNumber="231">Ecology and distribution.</emphasis>
|
||
<taxonomicName id="4C694D3ADB50FF89FE66F81AD1DD8CBE" authorityName="X. J. Wang" authorityYear="2023" box="[504,746,1996,2022]" class="Agaricomycetes" genus="Serendipita" kingdom="Fungi" order="Auriculariales" pageId="2" pageNumber="231" phylum="Basidiomycota" rank="species" species="officinale">
|
||
<emphasis id="B91DEAABDB50FF89FE66F81AD1DD8CBE" box="[504,746,1996,2022]" italics="true" pageId="2" pageNumber="231">Serendipita officinale</emphasis>
|
||
</taxonomicName>
|
||
is described from a strain isolated from protocorms and adult roots of
|
||
<taxonomicName id="4C694D3ADB50FF89FF7BF827D2458352" authorityName="X. J. Wang" authorityYear="2023" box="[229,370,2032,2058]" class="Agaricomycetes" genus="Serendipita" kingdom="Fungi" order="Auriculariales" pageId="2" pageNumber="231" phylum="Basidiomycota" rank="species" species="officinale">
|
||
<emphasis id="B91DEAABDB50FF89FF7BF827D2458352" box="[229,370,2032,2058]" italics="true" pageId="2" pageNumber="231">D. officinale</emphasis>
|
||
</taxonomicName>
|
||
. Its actual geographic distribution is yet to be defined.
|
||
</paragraph>
|
||
</subSubSection>
|
||
</treatment>
|
||
</document> |