262 lines
16 KiB
XML
262 lines
16 KiB
XML
<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.65.49769" ID-GBIF-Dataset="32ef1774-1130-476b-b748-43cfd0c6d421" ID-PMC="PMC7125235" ID-Pensoft-Pub="1314-4049-65-119" ID-Pensoft-UUID="DC309D63B600547DBAE4652F3B360041" ID-PubMed="32269482" ModsDocID="1314-4049-65-119" checkinTime="1585276182215" checkinUser="pensoft" docAuthor="Xu, Li, Bao, Dan-Feng, Luo, Zong-Long, Su, Xi-Jun, Shen, Hong-Wei & Su, Hong-Yan" docDate="2020" docId="2905DCA792E55C62A22EAE1058358E0E" docLanguage="en" docName="MycoKeys 65: 119-138" docOrigin="MycoKeys 65" docSource="http://dx.doi.org/10.3897/mycokeys.65.49769" docTitle="Tingoldiago clavata D. F. Bao, L. Xu & H. Y. Su 2020, sp. nov." docType="treatment" docVersion="4" id="DC309D63B600547DBAE4652F3B360041" lastPageNumber="119" masterDocId="DC309D63B600547DBAE4652F3B360041" masterDocTitle="Lignicolous freshwater ascomycota from Thailand: Phylogenetic and morphological characterisation of two new freshwater fungi: Tingoldiago hydei sp. nov. and T. clavata sp. nov. from Eastern Thailand" masterLastPageNumber="138" masterPageNumber="119" pageNumber="119" updateTime="1668136416202" updateUser="ExternalLinkService">
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<mods:mods xmlns:mods="http://www.loc.gov/mods/v3">
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<mods:titleInfo>
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<mods:title>Lignicolous freshwater ascomycota from Thailand: Phylogenetic and morphological characterisation of two new freshwater fungi: Tingoldiago hydei sp. nov. and T. clavata sp. nov. from Eastern Thailand</mods:title>
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</mods:titleInfo>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Xu, Li</mods:namePart>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Bao, Dan-Feng</mods:namePart>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Luo, Zong-Long</mods:namePart>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Su, Xi-Jun</mods:namePart>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Shen, Hong-Wei</mods:namePart>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Su, Hong-Yan</mods:namePart>
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</mods:name>
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<mods:typeOfResource>text</mods:typeOfResource>
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<mods:relatedItem type="host">
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<mods:titleInfo>
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<mods:title>MycoKeys</mods:title>
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</mods:titleInfo>
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<mods:part>
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<mods:date>2020</mods:date>
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<mods:detail type="volume">
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<mods:number>65</mods:number>
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</mods:detail>
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<mods:extent unit="page">
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<mods:start>119</mods:start>
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<mods:end>138</mods:end>
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</mods:extent>
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</mods:part>
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</mods:relatedItem>
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<mods:location>
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<mods:url>http://dx.doi.org/10.3897/mycokeys.65.49769</mods:url>
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</mods:location>
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<mods:classification>journal article</mods:classification>
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<mods:identifier type="DOI">http://dx.doi.org/10.3897/mycokeys.65.49769</mods:identifier>
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<mods:identifier type="Pensoft-Pub">1314-4049-65-119</mods:identifier>
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<mods:identifier type="Pensoft-UUID">DC309D63B600547DBAE4652F3B360041</mods:identifier>
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</mods:mods>
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<treatment ID-GBIF-Taxon="163297227" LSID="urn:lsid:plazi:treatment:2905DCA792E55C62A22EAE1058358E0E" httpUri="http://treatment.plazi.org/id/2905DCA792E55C62A22EAE1058358E0E" lastPageNumber="119" pageId="0" pageNumber="119">
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<subSubSection pageId="0" pageNumber="119" type="nomenclature">
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<paragraph pageId="0" pageNumber="119">
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<taxonomicName LSID="2905DCA7-92E5-5C62-A22E-AE1058358E0E" authority="D. F. Bao, L. Xu & H. Y. Su" authorityName="D. F. Bao, L. Xu & H. Y. Su" authorityYear="2020" class="Dothideomycetes" family="Lentitheciaceae" genus="Tingoldiago" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Tingoldiago clavata" order="Pleosporales" pageId="0" pageNumber="119" phylum="Ascomycota" rank="species" species="clavata" status="sp. nov.">Tingoldiago clavata D.F. Bao, L. Xu & H.Y. Su</taxonomicName>
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<taxonomicNameLabel pageId="0" pageNumber="119">sp. nov.</taxonomicNameLabel>
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<figureCitation captionStart="Figure 3" captionStartId="F3" captionText="Figure 3. Tingoldiago clavata (MFLU 19 - 2843, holotype). a-c ascomata on wood d section of ascoma e ostiole f peridium g pseudoparaphyses h-l asci m-r ascospores s vegetative hyphae in culture t, u culture on PDA from surface and reverse. Scale bars: 50 μm (d, e), 20 μm (f-l), 10 μm (m-s)." figureDoi="10.3897/mycokeys.65.49769.figure3" httpUri="https://binary.pensoft.net/fig/394431" pageId="0" pageNumber="119">Figure 3</figureCitation>
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="119" type="etymology">
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<paragraph pageId="0" pageNumber="119">Etymology.</paragraph>
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<paragraph pageId="0" pageNumber="119">Referring to the clavate ascospores of this fungus.</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="119" type="holotype">
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<paragraph pageId="0" pageNumber="119">Holotype.</paragraph>
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<paragraph pageId="0" pageNumber="119">Thailand, That Phanom, Nakhon Phanom, on submerged decaying wood, 13 November 2018, D.F. Bao, B-161 (MFLU 19-2843, holotype), ex-type culture, MFLUCC 19-0496.</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="119" type="description">
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<paragraph pageId="0" pageNumber="119">Description.</paragraph>
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<paragraph pageId="0" pageNumber="119">
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<taxonomicName genus="Saprobic" lsidName="Saprobic" pageId="0" pageNumber="119" rank="genus">
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<emphasis italics="true" pageId="0" pageNumber="119">Saprobic</emphasis>
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</taxonomicName>
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on submerged decaying wood.
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<emphasis bold="true" italics="true" pageId="0" pageNumber="119">Sexual morph</emphasis>
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:
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<emphasis italics="true" pageId="0" pageNumber="119">Ascomata</emphasis>
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145-210
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<normalizedToken originalValue="×">x</normalizedToken>
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145-195
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<normalizedToken originalValue="μm">μm</normalizedToken>
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(
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<emphasis italics="true" pageId="0" pageNumber="119">x̄</emphasis>
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= 175
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<normalizedToken originalValue="×">x</normalizedToken>
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169
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<normalizedToken originalValue="μm">μm</normalizedToken>
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, n = 10), immersed to semi-immersed, gregarious, scattered, erumpentia, depressed globose to conical with a flattened base, dark brown to black, as dark spots on host surface.
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<emphasis italics="true" pageId="0" pageNumber="119">Ostiole</emphasis>
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central, round to papillate, short, crest-like, dark brown.
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<emphasis italics="true" pageId="0" pageNumber="119">Peridium</emphasis>
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28-47
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<normalizedToken originalValue="μm">μm</normalizedToken>
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wide, comprising several layers, pale brown to brown cells of
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<emphasis italics="true" pageId="0" pageNumber="119">textura anngularis</emphasis>
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.
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<emphasis italics="true" pageId="0" pageNumber="119">Hamathecium</emphasis>
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comprising 1.5-2.0
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<normalizedToken originalValue="μm">μm</normalizedToken>
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(n = 30) wide, numerous, branched, septate, hyaline, cellular pseudoparaphyses.
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<emphasis italics="true" pageId="0" pageNumber="119">Asci</emphasis>
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110-148
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<normalizedToken originalValue="×">x</normalizedToken>
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20-27
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<normalizedToken originalValue="μm">μm</normalizedToken>
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(
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<emphasis italics="true" pageId="0" pageNumber="119">x̄</emphasis>
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= 129
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<normalizedToken originalValue="×">x</normalizedToken>
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23
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<normalizedToken originalValue="μm">μm</normalizedToken>
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, n = 20), 8-spored, bitunicate, fissitunicate, cylindrical-clavate, rounded at apex, with a short pedicellate.
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<emphasis italics="true" pageId="0" pageNumber="119">Ascospores</emphasis>
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48-51
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<normalizedToken originalValue="×">x</normalizedToken>
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7.5-9
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<normalizedToken originalValue="μm">μm</normalizedToken>
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(
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<emphasis italics="true" pageId="0" pageNumber="119">x̄</emphasis>
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= 50.5
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<normalizedToken originalValue="×">x</normalizedToken>
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8.5
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<normalizedToken originalValue="μm">μm</normalizedToken>
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, n = 30), overlapping, 2-3-seriate, clavate, with round ends, straight, uniseptate, deeply constricted at septum, hyaline, with broad and short upper cells 16.6-18.9
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<normalizedToken originalValue="×">x</normalizedToken>
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7.8-9.0
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<normalizedToken originalValue="μm">μm</normalizedToken>
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(
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<emphasis italics="true" pageId="0" pageNumber="119">x̄</emphasis>
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= 17.7
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<normalizedToken originalValue="×">x</normalizedToken>
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8.4
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<normalizedToken originalValue="μm">μm</normalizedToken>
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, n = 30), narrow and long lower cells 30-32.9
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<normalizedToken originalValue="×">x</normalizedToken>
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6.5-8.0
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<normalizedToken originalValue="μm">μm</normalizedToken>
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(
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<emphasis italics="true" pageId="0" pageNumber="119">x̄</emphasis>
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= 31.5
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<normalizedToken originalValue="×">x</normalizedToken>
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7.3
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<normalizedToken originalValue="μm">μm</normalizedToken>
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, n = 30), tapering towards the end, guttulate, smooth, 2-4 equatorial appendages at the septum and surrounded by a fusiform gelatinous, sheath.
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<emphasis bold="true" italics="true" pageId="0" pageNumber="119">Asexual morph</emphasis>
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：Undetermined.
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</paragraph>
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<caption doi="10.3897/mycokeys.65.49769.figure3" httpUri="https://binary.pensoft.net/fig/394431" pageId="0" pageNumber="119" start="Figure 3" startId="F3">
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<paragraph pageId="0" pageNumber="119">
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<emphasis bold="true" pageId="0" pageNumber="119">Figure 3.</emphasis>
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<taxonomicName authorityName="D. F. Bao, L. Xu & H. Y. Su" authorityYear="2020" class="Dothideomycetes" genus="Tingoldiago" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Tingoldiago clavata" order="Pleosporales" pageId="0" pageNumber="119" phylum="Ascomycota" rank="species" species="clavata">
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<emphasis italics="true" pageId="0" pageNumber="119">Tingoldiago clavata</emphasis>
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</taxonomicName>
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(MFLU 19-2843, holotype).
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<emphasis bold="true" pageId="0" pageNumber="119">a-c</emphasis>
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ascomata on wood
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<emphasis bold="true" pageId="0" pageNumber="119">d</emphasis>
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section of ascoma
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<emphasis bold="true" pageId="0" pageNumber="119">e</emphasis>
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ostiole
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<emphasis bold="true" pageId="0" pageNumber="119">f</emphasis>
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peridium
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<emphasis bold="true" pageId="0" pageNumber="119">g</emphasis>
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pseudoparaphyses
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<emphasis bold="true" pageId="0" pageNumber="119">h-l</emphasis>
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asci
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<emphasis bold="true" pageId="0" pageNumber="119">m-r</emphasis>
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ascospores
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<emphasis bold="true" pageId="0" pageNumber="119">s</emphasis>
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vegetative hyphae in culture
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<emphasis bold="true" pageId="0" pageNumber="119">t, u</emphasis>
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culture on PDA from surface and reverse. Scale bars: 50
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<normalizedToken originalValue="μm">μm</normalizedToken>
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(
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<emphasis bold="true" pageId="0" pageNumber="119">d, e</emphasis>
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), 20
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<normalizedToken originalValue="μm">μm</normalizedToken>
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(
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<emphasis bold="true" pageId="0" pageNumber="119">f-l</emphasis>
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), 10
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<normalizedToken originalValue="μm">μm</normalizedToken>
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(
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<emphasis bold="true" pageId="0" pageNumber="119">m-s</emphasis>
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).
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</paragraph>
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</caption>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="119" type="description">
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<paragraph pageId="0" pageNumber="119">Culture characteristics.</paragraph>
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<paragraph pageId="0" pageNumber="119">Ascospores germinating on PDA within 24 hours. Colonies on MEA effuse, velvety, greyish-white to dark brown from above and below, reaching 2.5-3 cm diameter within 30 days at room temperature under natural light, composed of subhyaline to brown, septate, smooth hyphae.</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="119" type="materials_examined">
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<paragraph pageId="0" pageNumber="119">Additional specimens examined.</paragraph>
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<paragraph pageId="0" pageNumber="119">Thailand, That Phanom, Nakhon Phanom, on submerged decaying wood, 13 November 2018, D.F. Bao, B160 (paratype: MFLU 19-2844; living culture, MFLUCC 19-0498); Thailand, That Phanom, Nakhon Phanom, on submerged decaying wood, 13 November 2018, D.F. Bao, B136 (paratype: MFLU 19-2845; living culture, MFLUCC 19-0495)</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="119" type="notes">
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<paragraph pageId="0" pageNumber="119">Notes.</paragraph>
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<paragraph pageId="0" pageNumber="119">
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<taxonomicName authorityName="D. F. Bao, L. Xu & H. Y. Su" authorityYear="2020" class="Dothideomycetes" genus="Tingoldiago" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Tingoldiago clavata" order="Pleosporales" pageId="0" pageNumber="119" phylum="Ascomycota" rank="species" species="clavata">
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<emphasis italics="true" pageId="0" pageNumber="119">Tingoldiago clavata</emphasis>
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</taxonomicName>
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resembles the type species,
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<taxonomicName lsidName="T. graminicola" pageId="0" pageNumber="119" rank="species" species="graminicola">
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<emphasis italics="true" pageId="0" pageNumber="119">T. graminicola</emphasis>
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</taxonomicName>
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in having bitunicate, cylindrical-clavate asci with a short pedicellate and clavate, hyaline, 1-septate, ascospores with broad upper cells, narrow lower cells. However, we can distinguish them by the size of ascomata and asci and the colour, septate and appendages of ascospores.
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<taxonomicName authorityName="D. F. Bao, L. Xu & H. Y. Su" authorityYear="2020" class="Dothideomycetes" genus="Tingoldiago" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Tingoldiago clavata" order="Pleosporales" pageId="0" pageNumber="119" phylum="Ascomycota" rank="species" species="clavata">
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<emphasis italics="true" pageId="0" pageNumber="119">Tingoldiago clavata</emphasis>
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</taxonomicName>
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has smaller ascomata (110-148
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<normalizedToken originalValue="×">x</normalizedToken>
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145-195 vs. 150-250
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<normalizedToken originalValue="×">x</normalizedToken>
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250-450
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<normalizedToken originalValue="μm">μm</normalizedToken>
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) and larger asci (110-148
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<normalizedToken originalValue="×">x</normalizedToken>
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20-27 vs. 87.5-122
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<normalizedToken originalValue="×">x</normalizedToken>
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18.25-25
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<normalizedToken originalValue="μm">μm</normalizedToken>
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). Moreover, ascopsores of
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<taxonomicName lsidName="T. clavata" pageId="0" pageNumber="119" rank="species" species="clavata">
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<emphasis italics="true" pageId="0" pageNumber="119">T. clavata</emphasis>
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</taxonomicName>
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are hyaline, uniseptate, with 2-4 equatorial appendages at the septum, while ascopspores of
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<taxonomicName lsidName="T. graminicola" pageId="0" pageNumber="119" rank="species" species="graminicola">
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<emphasis italics="true" pageId="0" pageNumber="119">T. graminicola</emphasis>
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</taxonomicName>
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are brown and 3-septate at maturity and lacking appendages at the septum. In addition, a comparison of the 491 nucleotides across the ITS gene region of
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<taxonomicName lsidName="T. clavata" pageId="0" pageNumber="119" rank="species" species="clavata">
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<emphasis italics="true" pageId="0" pageNumber="119">T. clavata</emphasis>
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</taxonomicName>
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and
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<taxonomicName lsidName="T. graminicola" pageId="0" pageNumber="119" rank="species" species="graminicola">
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<emphasis italics="true" pageId="0" pageNumber="119">T. graminicola</emphasis>
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</taxonomicName>
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reveals 25 base-pair differences and therefore provides further evidence to introduce
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<taxonomicName lsidName="T. clavata" pageId="0" pageNumber="119" rank="species" species="clavata">
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<emphasis italics="true" pageId="0" pageNumber="119">T. clavata</emphasis>
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</taxonomicName>
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as a new species as recommended by
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<bibRefCitation DOI="https://doi.org/10.5943/mycosphere/7/11/4" author="Jeewon, R" journalOrPublisher="Mycosphere" pageId="0" pageNumber="119" pagination="1669 - 1677" refId="B19" refString="Jeewon, R, Hyde, KD, 2016. Establishing species boundaries and new taxa among fungi: recommendations to resolve taxonomic ambiguities. Mycosphere 7: 1669 - 1677, DOI: https://doi.org/10.5943/mycosphere/7/11/4" title="Establishing species boundaries and new taxa among fungi: recommendations to resolve taxonomic ambiguities." url="https://doi.org/10.5943/mycosphere/7/11/4" volume="7" year="2016">Jeewon and Hyde (2016)</bibRefCitation>
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.
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</paragraph>
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</subSubSection>
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</treatment>
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</document> |