treatments-xml/data/E5/31/66/E5316619D55E4C46FF28FCEBA642FE08.xml

<document id="A79C45B75A87D032F27378CB85D94BDE" ID-CLB-Dataset="133816" ID-DOI="10.11646/zootaxa.5178.6.1" ID-GBIF-Dataset="b66aa5c6-906c-4e7b-9055-1dfb5ed4f262" ID-ISSN="1175-5326" ID-Zenodo-Dep="7037391" ID-ZooBank="DB0FBF00-47CE-4DD8-B239-BEA81464D38F" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.tables_requiresApprovalFor="existingObjects,plazi" IM.taxonomicNames_approvedBy="felipe" checkinTime="1661935085724" checkinUser="plazi" docAuthor="Varela-Soto, Donald, Abarca, Juan G., Brenes-Mora, Esteban, Aspinall, Valeria, Leenders, Twan &amp; Shepack, Alex" docDate="2022" docId="E5316619D55E4C46FF28FCEBA642FE08" docLanguage="en" docName="zootaxa.5178.6.1.pdf" docOrigin="Zootaxa 5178 (6)" docStyle="DocumentStyle:647186512141C8FC8976D5BCC54AEB7D.9:Zootaxa.2013-.journal_article" docStyleId="647186512141C8FC8976D5BCC54AEB7D" docStyleName="Zootaxa.2013-.journal_article" docStyleVersion="9" docTitle="Tlalocohyla celeste Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack 2022, sp. nov." docType="treatment" docVersion="10" lastPageNumber="522" masterDocId="19081E61D55A4C53FFBFFFF4A466FFFD" masterDocTitle="A new species of brilliant green frog of the genus Tlalocohyla (Anura, Hylidae) hiding between two volcanoes of northern Costa Rica" masterLastPageNumber="531" masterPageNumber="501" pageNumber="505" updateTime="1699374485303" updateUser="ExternalLinkService" zenodo-license-document="CLOSED">
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<mods:title id="3C3C018FA7FE1BB25989CBC9514A963D">A new species of brilliant green frog of the genus Tlalocohyla (Anura, Hylidae) hiding between two volcanoes of northern Costa Rica</mods:title>
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<mods:namePart id="6A4F3399F381196D952778E515D5DA0E">Varela-Soto, Donald</mods:namePart>
<mods:affiliation id="8DB045D142D203FA193858FF6F8DCDDD">Tapir Valley Nature Reserve, Bijagua, Upala, Costa Rica.</mods:affiliation>
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<mods:namePart id="8FE040DF2864C90D1399B4BF8CEB3C18">Abarca, Juan G.</mods:namePart>
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<mods:affiliation id="A2B1EB6C9241204F3DD24672A564771F">Laboratorio de Recursos Naturales y Vida Silvestre, Escuela de Ciencias Biológicas, Universidad Nacional, Heredia, Costa Rica. barcazajuan @ gmail. com; https: // orcid. org / 0000 - 0002 - 1261 - 4400 &amp; Costa Rica Wildlife Foundation, San José, Costa Rica.</mods:affiliation>
<mods:nameIdentifier id="5C91965106791A50215EAD7131CE469C" type="email">barcazajuan@gmail.com</mods:nameIdentifier>
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<mods:namePart id="A2562687A432823256F75B0FB320BCE7">Brenes-Mora, Esteban</mods:namePart>
<mods:affiliation id="31E13F3DC0A0276D452EC2AE13319CF6">Costa Rica Wildlife Foundation, San José, Costa Rica. &amp; Re: Wild, PO Box 129, Austin, Texas 78767, USA.</mods:affiliation>
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<mods:namePart id="2AF71AA26702DF39B5413DA08F3B1B17">Aspinall, Valeria</mods:namePart>
<mods:affiliation id="38D391DB7A005B4A51380DB2D12B422D">Costa Rica Wildlife Foundation, San José, Costa Rica.</mods:affiliation>
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<mods:namePart id="FA2E32063ACCE0D97EE6DE6E0250D6C2">Leenders, Twan</mods:namePart>
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<mods:affiliation id="84090EAE50A6D9DCEE922D081F349C19">Division of Vertebrate Zoology, Yale Peabody Museum of Natural History, 170 Whitney Avenue, New Haven, CT, 06520, USA. twanleenders @ scinax. com; https: // orcid. org / 0000 - 0003 - 4166 - 5001</mods:affiliation>
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<mods:namePart id="B12B80BD116A2C979E9C24EB46D716BA">Shepack, Alex</mods:namePart>
<mods:affiliation id="023BC82487314D4F37259EB3951B359F">Department of Biological Sciences, University of Notre Dame, South Bend, IN 46556, USA.</mods:affiliation>
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<taxonomicName id="AA98AC8CD55E4C57FF28FCEBA518FCC7" authority="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack, 2022" authorityName="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack" authorityYear="2022" box="[151,382,799,826]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="4" pageNumber="505" phylum="Chordata" rank="species" species="celeste" status="sp. nov.">
<emphasis id="5FEC0B1DD55E4C57FF28FCEBA518FCC7" bold="true" box="[151,382,799,826]" italics="true" pageId="4" pageNumber="505">Tlalocohyla celeste</emphasis>
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<materialsCitation id="DDF0DD52D55E4C57FF28FCB7A777FCA0" ID-GBIF-Occurrence="3897296301" box="[151,785,835,862]" location="Valley Tree Frog" municipality="Tapir Valley" pageId="4" pageNumber="505" specimenCount="1" stateProvince="Valle">
<collectingMunicipality id="8D434D75D55E4C57FF28FCB7A546FCA0" box="[151,288,835,861]" pageId="4" pageNumber="505">
Tapir 
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Tree Frog, Rana Arbórea del 
<collectingRegion id="AF5C19EDD55E4C57FDD2FCB7A6C1FCA0" box="[621,679,835,861]" country="Honduras" name="Valle" pageId="4" pageNumber="505">Valle</collectingRegion>
del Tapir
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<paragraph id="6D27D70FD55E4C57FF28FC93A549FC7C" blockId="4.[151,785,799,897]" box="[151,303,871,897]" pageId="4" pageNumber="505">
(
<figureCitation id="F5A3CB8AD55E4C57FF20FC93A541FC7C" box="[159,295,871,897]" captionStart-0="FIGURE 2" captionStart-1="FIGURE 3" captionStart-2="FIGURE 4" captionStart-3="FIGURE 5" captionStart-4="FIGURE 6" captionStart-5="FIGURE 7" captionStartId-0="6.[152,255,1472,1496]" captionStartId-1="7.[152,255,1932,1956]" captionStartId-2="8.[152,255,1910,1934]" captionStartId-3="9.[152,255,1943,1967]" captionStartId-4="10.[152,255,1628,1652]" captionStartId-5="11.[152,255,1910,1934]" captionTargetBox-0="[152,1436,885,1449]" captionTargetBox-1="[151,1435,309,1890]" captionTargetBox-2="[172,1415,182,1885]" captionTargetBox-3="[171,1415,193,1903]" captionTargetBox-4="[152,1435,181,1604]" captionTargetBox-5="[154,1435,456,1884]" captionTargetId-0="figure-359@6.[152,1436,885,1449]" captionTargetId-1="figure-84@7.[151,1436,309,1908]" captionTargetId-2="figure-29@8.[172,1415,181,1886]" captionTargetId-3="figure-18@9.[152,1434,181,1920]" captionTargetId-4="figure-29@10.[151,1436,181,1604]" captionTargetId-5="figure-148@11.[151,1435,453,1887]" captionTargetPageId-0="6" captionTargetPageId-1="7" captionTargetPageId-2="8" captionTargetPageId-3="9" captionTargetPageId-4="10" captionTargetPageId-5="11" captionText-0="FIGURE 2. Holotype of Tlalocohyla celeste sp. nov. (UCR 23700). (A) Dorsal view. (B) Ventral view." captionText-1="FIGURE 3. Holotype of Tlalocohyla celeste sp. nov. (UCR 23700) (A) Dorsal view of head in living specimen. (B) Lateral view of head in preserved specimen. (C) Lateral view in preserved specimen, note the tympanum. Scale bar = 2 mm. (D) Right hand in ventral view. (E) Right foot in ventral view." captionText-2="FIGURE 4. Variation in dorsal pattern of Tlalocohyla celeste sp. nov. (A–B) male holotype of Tlalocohyla celeste sp. nov. (UCR 23700) in life. (C) Size differences between females and males. (D) Typical nocturnal pattern of males. (E) Female with the oocytes visible by transparency in the body. (F) Diurnal pattern of adults, males and females, note the white iris chance. (G) Juvenile. (H) Diurnal pattern with red-brown iris." captionText-3="FIGURE 5. Variation and diagnostic characteristics in Tlalocohyla celeste sp. nov. (A-B) pattern change and green tones change of two uncollected males. (C) Ventral view in diurnal pattern. (D) ventral view of female (UCR 23701), note green bones (black arrow) and white peritoneum (blue arrow). (E) Spotted pattern in juvenile. (F) Axillary membrane (red arrow)." captionText-4="FIGURE 6. Ventral view Tlalocohyla celeste sp. nov. (A) Ventrals skin texture, and cloacal view. (B) Liver of recently preserved specimen (ECB-Anf-f50-08-01,01.). (C) Nuptial pads of the left hand in Tlalocohyla celeste sp. nov. male holotype (UCR 23700), in life, and preserved." captionText-5="FIGURE 7. Vocal sac of uncollected males of Tlalocohyla celeste sp. nov. (A) Ventral view and vocal sac deflated. (B–D). Calling males perched on vegetation in different positions, note the vocal sac extension." figureDoi-0="http://doi.org/10.5281/zenodo.7037395" figureDoi-1="http://doi.org/10.5281/zenodo.7037397" figureDoi-2="http://doi.org/10.5281/zenodo.7037399" figureDoi-3="http://doi.org/10.5281/zenodo.7037406" figureDoi-4="http://doi.org/10.5281/zenodo.7037410" figureDoi-5="http://doi.org/10.5281/zenodo.7037412" httpUri-0="https://zenodo.org/record/7037395/files/figure.png" httpUri-1="https://zenodo.org/record/7037397/files/figure.png" httpUri-2="https://zenodo.org/record/7037399/files/figure.png" httpUri-3="https://zenodo.org/record/7037406/files/figure.png" httpUri-4="https://zenodo.org/record/7037410/files/figure.png" httpUri-5="https://zenodo.org/record/7037412/files/figure.png" pageId="4" pageNumber="505">Figures 2–7</figureCitation>
)
</paragraph>
</subSubSection>
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<paragraph id="6D27D70FD55E4C57FF28FC5BA5B7FBEF" blockId="4.[151,1437,943,1942]" pageId="4" pageNumber="505">
<materialsCitation id="DDF0DD52D55E4C57FF28FC5BA5B7FBEF" ID-GBIF-Occurrence="3897296303" accessionNumber="UCR 23700" collectingDate="2021-09-13" collectorName="Juan G. Abarca &amp; Valeria Aspinall &amp; Donald Varela &amp; Esteban Brenes." country="Costa Rica" county="Upala" elevation="660" location="Tapir Valley Nature Reserve" municipality="Bijagua" pageId="4" pageNumber="505" specimenCount="1" stateProvince="Alajuela" typeStatus="holotype">
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<typeStatus id="B22369ADD55E4C57FF28FC5BA560FC34" box="[151,262,943,969]" pageId="4" pageNumber="505" type="holotype">Holotype</typeStatus>
.
</emphasis>
UCR 23700, an adult male from 
<collectingCountry id="158F979FD55E4C57FD25FC5BA77DFC34" box="[666,795,943,969]" name="Costa Rica" pageId="4" pageNumber="505">Costa Rica</collectingCountry>
, 
<collectingRegion id="AF5C19EDD55E4C57FC97FC5BA7ECFC34" box="[808,906,943,969]" country="Costa Rica" name="Alajuela" pageId="4" pageNumber="505">Alajuela</collectingRegion>
, 
<collectingCounty id="8446AF83D55E4C57FC27FC5BA7B8FC34" box="[920,990,943,969]" pageId="4" pageNumber="505">Upala</collectingCounty>
, 
<collectingMunicipality id="8D434D75D55E4C57FC52FC5BA021FC34" box="[1005,1095,943,969]" pageId="4" pageNumber="505">Bijagua</collectingMunicipality>
, 
<location id="684781D4D55E4C57FBEAFC5BA1FAFC37" LSID="urn:lsid:plazi:treatment:E5316619D55E4C46FF28FCEBA642FE08:684781D4D55E4C57FBEAFC5BA1FAFC37" box="[1109,1436,943,970]" country="Costa Rica" county="Upala" municipality="Bijagua" name="Tapir Valley Nature Reserve" pageId="4" pageNumber="505" stateProvince="Alajuela">Tapir Valley Nature Reserve</location>
(10.72˚ N, 85.01˚ W; 
<elevation id="E6B5303CD55E4C57FE2FFC27A662FC10" box="[400,516,979,1006]" metricMagnitude="2" metricUnit="m" metricValue="6.6" pageId="4" pageNumber="505" unit="m" value="660.0">
<quantity id="AA607AEAD55E4C57FE2FFC27A5BFFC13" box="[400,473,979,1006]" metricMagnitude="2" metricUnit="m" metricValue="6.6" pageId="4" pageNumber="505" unit="m" value="660.0">660 m</quantity>
asl
</elevation>
), collected on 
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<collectingDate id="09620827D55E4C57FD12FC27A7F2FC10" box="[685,916,979,1005]" pageId="4" pageNumber="505" value="2021-09-13">September 13, 2021</collectingDate>
</date>
, by 
<collectorName id="C06DB2D9D55E4C57FC79FC20A01EFC10" box="[966,1144,979,1006]" pageId="4" pageNumber="505">Juan G. Abarca</collectorName>
, 
<collectorName id="C06DB2D9D55E4C57FB3BFC27A15BFC10" box="[1156,1341,979,1005]" pageId="4" pageNumber="505">Valeria Aspinall</collectorName>
, 
<collectorName id="C06DB2D9D55E4C57FAF5FC27A484FBEC" pageId="4" pageNumber="505">Donald Varela</collectorName>
, and 
<collectorName id="C06DB2D9D55E4C57FEA3FC03A5B7FBEF" box="[284,465,1015,1042]" pageId="4" pageNumber="505">Esteban Brenes.</collectorName>
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</paragraph>
<paragraph id="6D27D70FD55E4C57FF78FBEFA7EAFBA4" blockId="4.[151,1437,943,1942]" pageId="4" pageNumber="505">
<emphasis id="5FEC0B1DD55E4C57FF78FBEFA51DFBC8" bold="true" box="[199,379,1051,1077]" pageId="4" pageNumber="505">Paratopotypes.</emphasis>
Two adult males, ECB-Anf-f50-08-01,01 and 
<collectionCode id="0B894FCAD55E4C57FCC3FBE8A7DFFBCB" box="[892,953,1052,1078]" country="USA" httpUri="http://biocol.org/urn:lsid:biocol.org:col:34880" lsid="urn:lsid:biocol.org:col:34880" name="Peabody Museum of Natural History" pageId="4" pageNumber="505" type="Museum">YPM</collectionCode>
13222, same data as 
<typeStatus id="B22369ADD55E4C57FB1EFBEFA161FBC8" box="[1185,1287,1051,1077]" pageId="4" pageNumber="505" type="holotype">holotype</typeStatus>
; 
<materialsCitation id="DDF0DD52D55E4C57FAAFFBEFA7EEFBA4" ID-GBIF-Occurrence="3897296302" accessionNumber="UCR 23701" collectingDate="2021-09-15" collectorName="Donald Varela" pageId="4" pageNumber="505" specimenCount="1">
UCR 23701, an adult female collected by 
<collectorName id="C06DB2D9D55E4C57FE65FBCBA61DFBA4" box="[474,635,1087,1113]" pageId="4" pageNumber="505">Donald Varela</collectorName>
on 
<date id="1926F1CFD55E4C57FD1BFBCBA7EEFBA4" box="[676,904,1087,1114]" pageId="4" pageNumber="505" value="2021-09-15">
<collectingDate id="09620827D55E4C57FD1BFBCBA7EEFBA4" box="[676,904,1087,1114]" pageId="4" pageNumber="505" value="2021-09-15">September 15, 2021</collectingDate>
</date>
</materialsCitation>
.
</paragraph>
<paragraph id="6D27D70FD55E4C57FF78FB97A778FB5F" blockId="4.[151,1437,943,1942]" pageId="4" pageNumber="505">
<emphasis id="5FEC0B1DD55E4C57FF78FB97A5C9FB80" bold="true" box="[199,431,1123,1149]" pageId="4" pageNumber="505">Generic placement.</emphasis>
Bayesian (
<figureCitation id="F5A3CB8AD55E4C57FD93FB97A608FB80" box="[556,622,1123,1149]" captionStart="FIGURE 1" captionStartId="5.[152,255,1943,1967]" captionTargetBox="[265,1311,191,1911]" captionTargetId="figure-18@5.[265,1323,181,1920]" captionTargetPageId="5" captionText="FIGURE 1. Bayesian phylogenetic tree within the Hylidae family based on 12S and 16S mitochondrial DNA genes. Posterior probabilities in nodes, asterisks represent support of&gt;95. Branches are collapsed to illustrate genus-level relationships, see expanded branches on Suppl. material. 2. The scale bar refers to the estimated substitutions per site." figureDoi="http://doi.org/10.5281/zenodo.7037393" httpUri="https://zenodo.org/record/7037393/files/figure.png" pageId="4" pageNumber="505">Fig. 1</figureCitation>
, Suppl. fig. 2) and Maximum likelihood analyses (Suppl. fig. 3) confirm the inclusion of the new species in the genus 
<taxonomicName id="AA98AC8CD55E4C57FD2EFB73A771FB5C" box="[657,791,1159,1185]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="4" pageNumber="505" phylum="Chordata" rank="genus">
<emphasis id="5FEC0B1DD55E4C57FD2EFB73A771FB5C" box="[657,791,1159,1185]" italics="true" pageId="4" pageNumber="505">Tlalocohyla</emphasis>
</taxonomicName>
.
</paragraph>
</subSubSection>
<subSubSection id="25828484D55E4C55FF78FB5FA652FE08" lastPageId="6" lastPageNumber="507" pageId="4" pageNumber="505" type="diagnosis">
<paragraph id="6D27D70FD55E4C57FF78FB5FA573FA3C" blockId="4.[151,1437,943,1942]" pageId="4" pageNumber="505">
<emphasis id="5FEC0B1DD55E4C57FF78FB5FA524FB38" bold="true" box="[199,322,1195,1221]" pageId="4" pageNumber="505">Diagnosis.</emphasis>
The new species can be diagnosed by the following combination of traits: (1) small size (adults males: 
<quantity id="AA607AEAD55E4C57FF58FB3BA551FB17" box="[231,311,1231,1258]" metricMagnitude="-2" metricUnit="m" metricValue="2.1" pageId="4" pageNumber="505" unit="mm" value="21.0">21 mm</quantity>
, females: 
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of SVL; n=4); (2) tympanic membrane not-evident; (3) vocal sac developed, single, subgular, extending to the pectoral region; (4) vomerine teeth absent; (5) dorsal color yellow-green (#103); (6) ventral skin transparent; (7) white peritoneum covering all internal organs; (8) presence of an incomplete sulfur white (#96) dorsolateral stripe that originates at the posterior edge of the orbit and extends posteriorly to a point more than halfway towards the insertion of each hindlimb, bordered above for its entire length by a diffuse mahogany red (34) stripe that extends anteriorly towards the tip of the snout; (9) presence of a small light cyan (#158) axillary membrane.
</paragraph>
<paragraph id="6D27D70FD55E4C57FF78FA3FA072F868" blockId="4.[151,1437,943,1942]" pageId="4" pageNumber="505">
Within its genus, 
<taxonomicName id="AA98AC8CD55E4C57FE2BFA3FA609FA18" authorityName="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack" authorityYear="2022" box="[404,623,1483,1509]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="4" pageNumber="505" phylum="Chordata" rank="species" species="celeste">
<emphasis id="5FEC0B1DD55E4C57FE2BFA3FA609FA18" box="[404,623,1483,1509]" italics="true" pageId="4" pageNumber="505">Tlalocohyla celeste</emphasis>
</taxonomicName>
can be differentiated from 
<taxonomicName id="AA98AC8CD55E4C57FC11FA38A048FA18" baseAuthorityName="Gunther" baseAuthorityYear="1901" box="[942,1070,1483,1509]" class="Amphibia" family="Hylidae" genus="Hyla" kingdom="Animalia" order="Anura" pageId="4" pageNumber="505" phylum="Chordata" rank="species" species="godmani">
<emphasis id="5FEC0B1DD55E4C57FC11FA38A048FA18" box="[942,1070,1483,1509]" italics="true" pageId="4" pageNumber="505">T. godmani</emphasis>
</taxonomicName>
and 
<taxonomicName id="AA98AC8CD55E4C57FBD5FA38A0B2FA18" baseAuthorityName="Gaige &amp; Stuart" baseAuthorityYear="1934" box="[1130,1236,1483,1509]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="4" pageNumber="505" phylum="Chordata" rank="species" species="loquax">
<emphasis id="5FEC0B1DD55E4C57FBD5FA38A0B2FA18" box="[1130,1236,1483,1509]" italics="true" pageId="4" pageNumber="505">T. loquax</emphasis>
</taxonomicName>
by the following characteristics (condition for 
<taxonomicName id="AA98AC8CD55E4C57FE5FFA04A62EF9F4" authorityName="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack" authorityYear="2022" box="[480,584,1519,1545]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="4" pageNumber="505" phylum="Chordata" rank="species" species="celeste">
<emphasis id="5FEC0B1DD55E4C57FE5FFA04A62EF9F4" box="[480,584,1519,1545]" italics="true" pageId="4" pageNumber="505">T. celeste</emphasis>
</taxonomicName>
in parentheses): maximum SVL 
<quantity id="AA607AEAD55E4C57FC05FA1BA06FF9F7" box="[954,1033,1519,1546]" metricMagnitude="-2" metricUnit="m" metricValue="4.5" pageId="4" pageNumber="505" unit="mm" value="45.0">45 mm</quantity>
(
<quantity id="AA607AEAD55E4C57FBA7FA1BA00AF9F4" box="[1048,1132,1519,1545]" metricMagnitude="-2" metricUnit="m" metricValue="2.4" pageId="4" pageNumber="505" unit="mm" value="24.0">24 mm</quantity>
); venter pale lemon yellow or creamy yellow (transparent ventral skin) and extensive axillary membrane (very small axillary membrane). 
<taxonomicName id="AA98AC8CD55E4C57FF28F9C3A516F9AC" authorityName="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack" authorityYear="2022" box="[151,368,1591,1617]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="4" pageNumber="505" phylum="Chordata" rank="species" species="celeste">
<emphasis id="5FEC0B1DD55E4C57FF28F9C3A516F9AC" box="[151,368,1591,1617]" italics="true" pageId="4" pageNumber="505">Tlalocohyla celeste</emphasis>
</taxonomicName>
is similar in overall appearance to 
<taxonomicName id="AA98AC8CD55E4C57FD46F9CCA706F9AC" baseAuthorityName="Boulenger" baseAuthorityYear="1902" box="[761,864,1591,1617]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="4" pageNumber="505" phylum="Chordata" rank="species" species="smithii">
<emphasis id="5FEC0B1DD55E4C57FD46F9CCA706F9AC" box="[761,864,1591,1617]" italics="true" pageId="4" pageNumber="505">T. smithii</emphasis>
</taxonomicName>
and 
<taxonomicName id="AA98AC8CD55E4C57FC28F9CCA78DF9AC" baseAuthorityName="Gunther" baseAuthorityYear="1901" box="[919,1003,1592,1617]" class="Amphibia" family="Hylidae" genus="Hyla" kingdom="Animalia" order="Anura" pageId="4" pageNumber="505" phylum="Chordata" rank="species" species="picta">
<emphasis id="5FEC0B1DD55E4C57FC28F9CCA78DF9AC" box="[919,1003,1592,1617]" italics="true" pageId="4" pageNumber="505">T. picta</emphasis>
</taxonomicName>
, but can be differentiated based on the following characteristics: from 
<taxonomicName id="AA98AC8CD55E4C57FE44F9A8A602F988" baseAuthorityName="Boulenger" baseAuthorityYear="1902" box="[507,612,1627,1653]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="4" pageNumber="505" phylum="Chordata" rank="species" species="smithii">
<emphasis id="5FEC0B1DD55E4C57FE44F9A8A602F988" box="[507,612,1627,1653]" italics="true" pageId="4" pageNumber="505">T. smithii</emphasis>
</taxonomicName>
by its smaller size, maximum SVL 
<quantity id="AA607AEAD55E4C57FC40F9AFA029F98B" box="[1023,1103,1627,1654]" metricMagnitude="-2" metricUnit="m" metricValue="3.0" pageId="4" pageNumber="505" unit="mm" value="30.0">30 mm</quantity>
(
<quantity id="AA607AEAD55E4C57FBE0F9AFA0D3F988" box="[1119,1205,1627,1653]" metricMagnitude="-2" metricUnit="m" metricValue="2.4" pageId="4" pageNumber="505" unit="mm" value="24.0">24 mm</quantity>
); pale yellow, tan or brilliant yellow dorsum (bright yellow-green with mahogany red spots); a thin complete white or cream dorsolateral stripe that is usually bordered below by a thin brown line (an incomplete sulphur white dorsolateral stripe bordered above by a diffuse mahogany red stripe); white belly (transparent); vomerine teeth present (absent); and from 
<taxonomicName id="AA98AC8CD55E4C57FA37F93CA4A9F8F8" baseAuthorityName="Gunther" baseAuthorityYear="1901" class="Amphibia" family="Hylidae" genus="Hyla" kingdom="Animalia" order="Anura" pageId="4" pageNumber="505" phylum="Chordata" rank="species" species="picta">
<emphasis id="5FEC0B1DD55E4C57FA37F93CA4A9F8F8" italics="true" pageId="4" pageNumber="505">T. picta</emphasis>
</taxonomicName>
by the relatively uniform yellowish tan dorsal coloration (bright yellow-green with mahogany red spots); presence of a complete white or cream dorsolateral stripe that may be bordered below by a poorly defined reddish, brown or gray band (an incomplete sulphur white dorsolateral stripe bordered above by a diffuse mahogany red stripe); white bones (green bones) and vocal sac yellow (brilliant light cyan). 
<taxonomicName id="AA98AC8CD55E4C57FBBEF8A3A0BCF88C" authorityName="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack" authorityYear="2022" box="[1025,1242,1879,1905]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="4" pageNumber="505" phylum="Chordata" rank="species" species="celeste">
<emphasis id="5FEC0B1DD55E4C57FBBEF8A3A0BCF88C" box="[1025,1242,1879,1905]" italics="true" pageId="4" pageNumber="505">Tlalocohyla celeste</emphasis>
</taxonomicName>
also differs from these two species in its advertisement call and tadpole morphology (see below).
</paragraph>
<caption id="39E78787D55F4C56FF27F863A0EDF805" ID-DOI="http://doi.org/10.5281/zenodo.7037393" ID-Zenodo-Dep="7037393" httpUri="https://zenodo.org/record/7037393/files/figure.png" pageId="5" pageNumber="506" startId="5.[152,255,1943,1967]" targetBox="[265,1311,191,1911]" targetPageId="5">
<paragraph id="6D27D70FD55F4C56FF27F863A0EDF805" blockId="5.[151,1436,1943,2040]" pageId="5" pageNumber="506">
<emphasis id="5FEC0B1DD55F4C56FF27F863A57FF852" bold="true" box="[152,281,1943,1967]" pageId="5" pageNumber="506">FIGURE 1.</emphasis>
Bayesian phylogenetic tree within the 
<taxonomicName id="AA98AC8CD55F4C56FD1BF86CA692F84D" baseAuthorityName="Rafinesque" baseAuthorityYear="1815" box="[676,756,1944,1968]" class="Amphibia" family="Hylidae" kingdom="Animalia" order="Anura" pageId="5" pageNumber="506" phylum="Chordata" rank="family">Hylidae</taxonomicName>
family based on 12S and 16S mitochondrial DNA genes. Posterior probabilities in nodes, asterisks represent support of&gt;95. Branches are collapsed to illustrate genus-level relationships, see expanded branches on Suppl. material. 2. The scale bar refers to the estimated substitutions per site.
</paragraph>
</caption>
<paragraph id="6D27D70FD55C4C55FF78FF63A652FE08" blockId="6.[151,1437,151,861]" pageId="6" pageNumber="507">
<taxonomicName id="AA98AC8CD55C4C55FF78FF63A5C5FF4C" authorityName="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack" authorityYear="2022" box="[199,419,151,177]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="6" pageNumber="507" phylum="Chordata" rank="species" species="celeste">
<emphasis id="5FEC0B1DD55C4C55FF78FF63A5C5FF4C" box="[199,419,151,177]" italics="true" pageId="6" pageNumber="507">Tlalocohyla celeste</emphasis>
</taxonomicName>
is among the smallest hylids in 
<collectingCountry id="158F979FD55C4C55FCA2FF63A7FAFF4C" box="[797,924,151,177]" name="Costa Rica" pageId="6" pageNumber="507">Costa Rica</collectingCountry>
, slightly smaller than 
<taxonomicName id="AA98AC8CD55C4C55FB22FF63A105FF4C" baseAuthorityName="Gaige" baseAuthorityYear="1929" box="[1181,1379,151,177]" class="Amphibia" family="Hylidae" genus="Isthmohyla" kingdom="Animalia" order="Anura" pageId="6" pageNumber="507" phylum="Chordata" rank="species" species="zeteki">
<emphasis id="5FEC0B1DD55C4C55FB22FF63A105FF4C" box="[1181,1379,151,177]" italics="true" pageId="6" pageNumber="507">Isthmohyla zeteki</emphasis>
</taxonomicName>
, and may be confused with other small hylid species present within its range. Members of the genus 
<taxonomicName id="AA98AC8CD55C4C55FB53FF4FA1FAFF28" authorityName="Fitzinger" authorityYear="1843" box="[1260,1436,187,213]" class="Amphibia" family="Hylidae" genus="Dendropsophus" kingdom="Animalia" order="Anura" pageId="6" pageNumber="507" phylum="Chordata" rank="genus">
<emphasis id="5FEC0B1DD55C4C55FB53FF4FA1FAFF28" box="[1260,1436,187,213]" italics="true" pageId="6" pageNumber="507">Dendropsophus</emphasis>
</taxonomicName>
are small tree frogs with an axillary membrane but none are green in coloration with transparent ventral skin. 
<taxonomicName id="AA98AC8CD55C4C55FF28FEF7A55CFEE0" box="[151,314,259,285]" pageId="6" pageNumber="507">
<emphasis id="5FEC0B1DD55C4C55FF28FEF7A484FEE0" box="[151,226,259,285]" italics="true" pageId="6" pageNumber="507">Scinax</emphasis>
species
</taxonomicName>
have a similar, rounded protruding lateral snout profile but are significantly larger in size and never have light dorsolateral markings. 
<taxonomicName id="AA98AC8CD55C4C55FDAAFED3A696FEBC" authorityName="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack" authorityYear="2022" box="[533,752,295,321]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="6" pageNumber="507" phylum="Chordata" rank="species" species="celeste">
<emphasis id="5FEC0B1DD55C4C55FDAAFED3A696FEBC" box="[533,752,295,321]" italics="true" pageId="6" pageNumber="507">Tlalocohyla celeste</emphasis>
</taxonomicName>
is most likely to be confused with juveniles of the syntopic 
<taxonomicName id="AA98AC8CD55C4C55FF28FEBFA55AFE98" baseAuthorityName="Fouquette" baseAuthorityYear="1961" box="[151,316,331,357]" class="Amphibia" family="Hylidae" genus="Boana" kingdom="Animalia" order="Anura" pageId="6" pageNumber="507" phylum="Chordata" rank="species" species="rufitela">
<emphasis id="5FEC0B1DD55C4C55FF28FEBFA55AFE98" box="[151,316,331,357]" italics="true" pageId="6" pageNumber="507">Boana rufitela</emphasis>
</taxonomicName>
, but the latter has a rounded snout in lateral profile and its white dorsolateral lines are complete, extending from the snout tip backwards to the vent, passing over top of the hindlimbs. These light markings fade and eventually disappear completely as young 
<taxonomicName id="AA98AC8CD55C4C55FDCCFE67A682FE50" baseAuthorityName="Fouquette" baseAuthorityYear="1961" box="[627,740,403,429]" class="Amphibia" family="Hylidae" genus="Boana" kingdom="Animalia" order="Anura" pageId="6" pageNumber="507" phylum="Chordata" rank="species" species="rufitela">
<emphasis id="5FEC0B1DD55C4C55FDCCFE67A682FE50" box="[627,740,403,429]" italics="true" pageId="6" pageNumber="507">B. rufitela</emphasis>
</taxonomicName>
mature. Because of its bright green coloration and transparent ventral skin, 
<taxonomicName id="AA98AC8CD55C4C55FE98FE43A5E8FE2C" authorityName="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack" authorityYear="2022" box="[295,398,439,465]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="6" pageNumber="507" phylum="Chordata" rank="species" species="celeste">
<emphasis id="5FEC0B1DD55C4C55FE98FE43A5E8FE2C" box="[295,398,439,465]" italics="true" pageId="6" pageNumber="507">T. celeste</emphasis>
</taxonomicName>
may be mistaken for a glass frog (family 
<taxonomicName id="AA98AC8CD55C4C55FCE2FE43A799FE2C" authorityName="Taylor" authorityYear="1951" box="[861,1023,439,465]" class="Amphibia" family="Centrolenidae" kingdom="Animalia" order="Anura" pageId="6" pageNumber="507" phylum="Chordata" rank="family">Centrolenidae</taxonomicName>
), however glass frogs invariably lack a pattern of light dorsolateral stripes.
</paragraph>
</subSubSection>
<subSubSection id="25828484D55C4C46FF78FE0AA5F8FF28" lastPageId="21" lastPageNumber="522" pageId="6" pageNumber="507" type="description">
<paragraph id="6D27D70FD55C4C55FF78FE0AA0C1FCA0" blockId="6.[151,1437,151,861]" pageId="6" pageNumber="507">
<emphasis id="5FEC0B1DD55C4C55FF78FE0AA58CFDE4" bold="true" box="[199,490,510,537]" pageId="6" pageNumber="507">
Description of 
<typeStatus id="B22369ADD55C4C55FEC3FE0BA583FDE4" box="[380,485,511,537]" pageId="6" pageNumber="507" type="holotype">holotype</typeStatus>
.
</emphasis>
An adult male with a SVL of 
<quantity id="AA607AEAD55C4C55FCEEFE0BA7A3FDE4" box="[849,965,511,537]" metricMagnitude="-2" metricUnit="m" metricValue="2.01" pageId="6" pageNumber="507" unit="mm" value="20.1">20.10 mm</quantity>
(
<figureCitation id="F5A3CB8AD55C4C55FC68FE0BA078FDE4" box="[983,1054,511,537]" captionStart="FIGURE 2" captionStartId="6.[152,255,1472,1496]" captionTargetBox="[152,1436,885,1449]" captionTargetId="figure-359@6.[152,1436,885,1449]" captionTargetPageId="6" captionText="FIGURE 2. Holotype of Tlalocohyla celeste sp. nov. (UCR 23700). (A) Dorsal view. (B) Ventral view." figureDoi="http://doi.org/10.5281/zenodo.7037395" httpUri="https://zenodo.org/record/7037395/files/figure.png" pageId="6" pageNumber="507">Fig. 2</figureCitation>
), in a good state of preservation, head slightly longer than wide, HL 38.3%, and HW 36.5% of SVL. Snout nearly rounded in dorsal view, rounded protruding in profile. Nostrils rounded, dorsolateral, directed laterally, located on slightly raised processes, IND 25.8% of HW. Canthus rostralis well defined and rounded. Loreal region concave. Eyes large and protuberant, ED 39.1% and 10.7% of HW and SVL, respectively. Pupil horizontally elliptical. Tympanum small, TD 23.0% of ED, tympanic membrane inevident, but tympanic annulus visible through the skin (
<figureCitation id="F5A3CB8AD55C4C55FBEFFD47A0CFFD30" box="[1104,1193,691,717]" captionStart="FIGURE 3" captionStartId="7.[152,255,1932,1956]" captionTargetBox="[151,1435,309,1890]" captionTargetId="figure-84@7.[151,1436,309,1908]" captionTargetPageId="7" captionText="FIGURE 3. Holotype of Tlalocohyla celeste sp. nov. (UCR 23700) (A) Dorsal view of head in living specimen. (B) Lateral view of head in preserved specimen. (C) Lateral view in preserved specimen, note the tympanum. Scale bar = 2 mm. (D) Right hand in ventral view. (E) Right foot in ventral view." figureDoi="http://doi.org/10.5281/zenodo.7037397" httpUri="https://zenodo.org/record/7037397/files/figure.png" pageId="6" pageNumber="507">Fig. 3C</figureCitation>
), tympanum matches character combination number 
<quantity id="AA607AEAD55C4C55FE50FD23A67EFD0C" box="[495,536,727,753]" metricMagnitude="-2" metricUnit="m" metricValue="5.08" pageId="6" pageNumber="507" unit="in" value="2.0">2 in</quantity>
<bibRefCitation id="0909AAFED55C4C55FDA2FD23A731FD0C" author="Lynch, J. D. &amp; Duellman, W. E." box="[541,855,727,753]" pageId="6" pageNumber="507" pagination="1 - 236" refId="ref13719" refString="Lynch, J. D. &amp; Duellman, W. E. (1997) Frogs of the genus Eleutherodactylus (Leptodactylidae) in western Ecuador: systematics, ecology, and biogeography. The University of Kansas Natural History Museum Special Publications, 23, 1 - 236." type="journal article" year="1997">Lynch and Duellman (1997)</bibRefCitation>
. Tongue ovoid, free laterally and posteriorly. Texture of tongue granular. Premaxillary and maxillary teeth present, no vomerine teeth visible between choanae. Choanae rounded anteriorly and widely separated. Vocal apertures are slit-like and elongated, nearly parallel the mandible. Vocal sac single and subgular, extends posteriorly to the pectoral region (
<figureCitation id="F5A3CB8AD55C4C55FC75FCB7A078FCA0" box="[970,1054,835,861]" captionStart="FIGURE 2" captionStartId="6.[152,255,1472,1496]" captionTargetBox="[152,1436,885,1449]" captionTargetId="figure-359@6.[152,1436,885,1449]" captionTargetPageId="6" captionText="FIGURE 2. Holotype of Tlalocohyla celeste sp. nov. (UCR 23700). (A) Dorsal view. (B) Ventral view." figureDoi="http://doi.org/10.5281/zenodo.7037395" httpUri="https://zenodo.org/record/7037395/files/figure.png" pageId="6" pageNumber="507">Fig. 2B</figureCitation>
and 
<figureCitation id="F5A3CB8AD55C4C55FBEAFCB7A0FFFCA0" box="[1109,1177,835,861]" captionStart="FIGURE 7" captionStartId="11.[152,255,1910,1934]" captionTargetBox="[154,1435,456,1884]" captionTargetId="figure-148@11.[151,1435,453,1887]" captionTargetPageId="11" captionText="FIGURE 7. Vocal sac of uncollected males of Tlalocohyla celeste sp. nov. (A) Ventral view and vocal sac deflated. (B–D). Calling males perched on vegetation in different positions, note the vocal sac extension." figureDoi="http://doi.org/10.5281/zenodo.7037412" httpUri="https://zenodo.org/record/7037412/files/figure.png" pageId="6" pageNumber="507">Fig. 7</figureCitation>
).
</paragraph>
<caption id="39E78787D55C4C55FF27FA34A0D0FA25" ID-DOI="http://doi.org/10.5281/zenodo.7037395" ID-Zenodo-Dep="7037395" box="[152,1206,1472,1496]" httpUri="https://zenodo.org/record/7037395/files/figure.png" pageId="6" pageNumber="507" startId="6.[152,255,1472,1496]" targetBox="[152,1436,885,1449]" targetPageId="6">
<paragraph id="6D27D70FD55C4C55FF27FA34A0D0FA25" blockId="6.[152,1206,1472,1496]" box="[152,1206,1472,1496]" pageId="6" pageNumber="507">
<emphasis id="5FEC0B1DD55C4C55FF27FA34A57EFA25" bold="true" box="[152,280,1472,1496]" pageId="6" pageNumber="507">FIGURE 2.</emphasis>
Holotype of 
<taxonomicName id="AA98AC8CD55C4C55FE20FA34A605FA25" authority="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack, 2022" authorityName="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack" authorityYear="2022" box="[415,611,1472,1496]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="6" pageNumber="507" phylum="Chordata" rank="species" species="celeste" status="sp. nov.">
<emphasis id="5FEC0B1DD55C4C55FE20FA34A605FA25" box="[415,611,1472,1496]" italics="true" pageId="6" pageNumber="507">Tlalocohyla celeste</emphasis>
</taxonomicName>
<emphasis id="5FEC0B1DD55C4C55FDD6FA34A6DCFA25" bold="true" box="[617,698,1472,1496]" pageId="6" pageNumber="507">
<taxonomicNameLabel id="44DFB666D55C4C55FDD6FA34A6DCFA25" box="[617,698,1472,1496]" pageId="6" pageNumber="507" rank="species">sp. nov.</taxonomicNameLabel>
</emphasis>
(UCR 23700). (A) Dorsal view. (B) Ventral view.
</paragraph>
</caption>
<paragraph id="6D27D70FD55C4C55FF78F9F2A6F2F874" blockId="6.[151,1437,1542,2037]" pageId="6" pageNumber="507">
Forelimbs not hypertrophied, upper arms and forearms slender, consistent throughout in its thickness. Hands proportionally large, HAL 33.1% of SVL. Lacks ulnar skin fold. A small axillary membrane present. Fingers robust, relative finger length I&lt;II&lt;IV&lt;III. Discs moderately expanded and round (Fin3DW 25% of ED). Finger discs are comparable in size but finger disc I is slightly smaller than others. Subarticular tubercles single, rounded or globose, large and slightly bifid in fingers III and IV. Supernumerary tubercles large, single and rounded. Accessory palmar tubercles numerous, rounded, large and globular; span the entire palmar area in a tightly packed way. Inner metacarpal tubercle (thenar) elongated, elliptical, globular and large; outer metacarpal tubercle (palmar) not clearly differentiated from the accessory palmar tubercles. White smooth nuptial pads without epidermal projections, at the base of the first finger, formed by a grouping of glandular acini, each circular or ovoid in shape (
<figureCitation id="F5A3CB8AD55C4C55FABBF8D2A13BF8BD" box="[1284,1373,1830,1856]" captionStart="FIGURE 6" captionStartId="10.[152,255,1628,1652]" captionTargetBox="[152,1435,181,1604]" captionTargetId="figure-29@10.[151,1436,181,1604]" captionTargetPageId="10" captionText="FIGURE 6. Ventral view Tlalocohyla celeste sp. nov. (A) Ventrals skin texture, and cloacal view. (B) Liver of recently preserved specimen (ECB-Anf-f50-08-01,01.). (C) Nuptial pads of the left hand in Tlalocohyla celeste sp. nov. male holotype (UCR 23700), in life, and preserved." figureDoi="http://doi.org/10.5281/zenodo.7037410" httpUri="https://zenodo.org/record/7037410/files/figure.png" pageId="6" pageNumber="507">Fig. 6C</figureCitation>
). The interdigital membrane between fingers I–II is vestigial, but it is slightly more developed between fingers II–III–IV. Webbing formula I3+–2- II 2-– 3-III3- –2-IV.
</paragraph>
<paragraph id="6D27D70FD55C4C54FF78F866A68BFF28" blockId="6.[151,1437,1542,2037]" lastBlockId="7.[151,1436,150,285]" lastPageId="7" lastPageNumber="508" pageId="6" pageNumber="507">
Hind limbs slender, THL slightly greater than FL and slightly smaller than TL. TL of about 57.0% of SVL, FL 43.5% of SVL. Toes robust. Relative length of toes I&lt;II&lt;III&lt;V&lt;IV. Discs rounded and expanded. Subarticular tubercles single, rounded and globular. Supernumerary tubercles small, single and rounded. Inner metatarsal tubercle medium-sized, elliptical; outer metatarsal tubercle absent. Webbing formula I 2 
<superScript id="9AED7A47D55D4C54FBACFF63A07EFF58" attach="none" box="[1043,1048,151,165]" fontSize="6" pageId="7" pageNumber="508">-</superScript>
–2 
<superScript id="9AED7A47D55D4C54FB8AFF63A058FF58" attach="right" box="[1077,1086,151,165]" fontSize="6" pageId="7" pageNumber="508">+</superScript>
II 
<superScript id="9AED7A47D55D4C54FBE7FF63A01CFF59" attach="none" box="[1112,1146,150,177]" fontSize="6" pageId="7" pageNumber="508">11/2</superScript>
–3 
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III 
<superScript id="9AED7A47D55D4C54FB75FF63A08AFF59" attach="none" box="[1226,1260,150,177]" fontSize="6" pageId="7" pageNumber="508">11/2</superScript>
–3 
<superScript id="9AED7A47D55D4C54FAB7FF63A16BFF58" attach="left" box="[1288,1293,151,165]" fontSize="6" pageId="7" pageNumber="508">-</superScript>
IV 2 
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– 
<superScript id="9AED7A47D55D4C54FADDFF63A1E6FF59" attach="left" box="[1378,1408,150,177]" fontSize="6" pageId="7" pageNumber="508">11/2</superScript>
V, free parts of toes fringed. Cloacal dermal fold absent.
</paragraph>
<paragraph id="6D27D70FD55D4C54FF78FF2BA773FEE0" blockId="7.[151,1436,150,285]" pageId="7" pageNumber="508">
Body covered in smooth skin throughout, except for the central region of the venter and the undersides of the thighs where skin texture is distinctly granular (
<figureCitation id="F5A3CB8AD55D4C54FD11FEF7A76EFEE0" box="[686,776,259,285]" captionStart="FIGURE 6" captionStartId="10.[152,255,1628,1652]" captionTargetBox="[152,1435,181,1604]" captionTargetId="figure-29@10.[151,1436,181,1604]" captionTargetPageId="10" captionText="FIGURE 6. Ventral view Tlalocohyla celeste sp. nov. (A) Ventrals skin texture, and cloacal view. (B) Liver of recently preserved specimen (ECB-Anf-f50-08-01,01.). (C) Nuptial pads of the left hand in Tlalocohyla celeste sp. nov. male holotype (UCR 23700), in life, and preserved." figureDoi="http://doi.org/10.5281/zenodo.7037410" httpUri="https://zenodo.org/record/7037410/files/figure.png" pageId="7" pageNumber="508">Fig. 6A</figureCitation>
).
</paragraph>
<caption id="39E78787D55D4C54FF27F878A6C2F811" ID-DOI="http://doi.org/10.5281/zenodo.7037397" ID-Zenodo-Dep="7037397" httpUri="https://zenodo.org/record/7037397/files/figure.png" pageId="7" pageNumber="508" startId="7.[152,255,1932,1956]" targetBox="[151,1435,309,1890]" targetPageId="7">
<paragraph id="6D27D70FD55D4C54FF27F878A6C2F811" blockId="7.[151,1436,1932,2028]" pageId="7" pageNumber="508">
<emphasis id="5FEC0B1DD55D4C54FF27F878A57FF859" bold="true" box="[152,281,1932,1956]" pageId="7" pageNumber="508">FIGURE 3.</emphasis>
Holotype of 
<taxonomicName id="AA98AC8CD55D4C54FE1CF878A60FF859" authority="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack, 2022" authorityName="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack" authorityYear="2022" box="[419,617,1932,1956]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="7" pageNumber="508" phylum="Chordata" rank="species" species="celeste" status="sp. nov.">
<emphasis id="5FEC0B1DD55D4C54FE1CF878A60FF859" box="[419,617,1932,1956]" italics="true" pageId="7" pageNumber="508">Tlalocohyla celeste</emphasis>
</taxonomicName>
<emphasis id="5FEC0B1DD55D4C54FDCFF878A6A4F859" bold="true" box="[624,706,1932,1956]" pageId="7" pageNumber="508">
<taxonomicNameLabel id="44DFB666D55D4C54FDCFF878A6A4F859" box="[624,706,1932,1956]" pageId="7" pageNumber="508" rank="species">sp. nov.</taxonomicNameLabel>
</emphasis>
(UCR 23700) (A) Dorsal view of head in living specimen. (B) Lateral view of head in preserved specimen. (C) Lateral view in preserved specimen, note the tympanum. Scale bar = 2 mm. (D) Right hand in ventral view. (E) Right foot in ventral view.
</paragraph>
</caption>
<caption id="39E78787D5524C5BFF27F882A6EBF807" ID-DOI="http://doi.org/10.5281/zenodo.7037399" ID-Zenodo-Dep="7037399" httpUri="https://zenodo.org/record/7037399/files/figure.png" pageId="8" pageNumber="509" startId="8.[152,255,1910,1934]" targetBox="[172,1415,182,1885]" targetPageId="8">
<paragraph id="6D27D70FD5524C5BFF27F882A6EBF807" blockId="8.[151,1437,1910,2042]" pageId="8" pageNumber="509">
<emphasis id="5FEC0B1DD5524C5BFF27F882A57CF873" bold="true" box="[152,282,1910,1934]" pageId="8" pageNumber="509">FIGURE 4.</emphasis>
Variation in dorsal pattern of 
<taxonomicName id="AA98AC8CD5524C5BFDECF882A77FF873" authority="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack, 2022" authorityName="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack" authorityYear="2022" box="[595,793,1910,1934]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="8" pageNumber="509" phylum="Chordata" rank="species" species="celeste" status="sp. nov.">
<emphasis id="5FEC0B1DD5524C5BFDECF882A77FF873" box="[595,793,1910,1934]" italics="true" pageId="8" pageNumber="509">Tlalocohyla celeste</emphasis>
</taxonomicName>
<emphasis id="5FEC0B1DD5524C5BFC9EF882A712F873" bold="true" box="[801,884,1910,1934]" pageId="8" pageNumber="509">
<taxonomicNameLabel id="44DFB666D5524C5BFC9EF882A712F873" box="[801,884,1910,1934]" pageId="8" pageNumber="509" rank="species">sp. nov.</taxonomicNameLabel>
</emphasis>
(A–B) male holotype of 
<taxonomicName id="AA98AC8CD5524C5BFBC3F882A124F873" authority="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack, 2022" authorityName="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack" authorityYear="2022" box="[1148,1346,1910,1934]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="8" pageNumber="509" phylum="Chordata" rank="species" species="celeste" status="sp. nov.">
<emphasis id="5FEC0B1DD5524C5BFBC3F882A124F873" box="[1148,1346,1910,1934]" italics="true" pageId="8" pageNumber="509">Tlalocohyla celeste</emphasis>
</taxonomicName>
<emphasis id="5FEC0B1DD5524C5BFAF5F882A1FAF873" bold="true" box="[1354,1436,1910,1934]" pageId="8" pageNumber="509">
<taxonomicNameLabel id="44DFB666D5524C5BFAF5F882A1FAF873" box="[1354,1436,1910,1934]" pageId="8" pageNumber="509" rank="species">sp. nov.</taxonomicNameLabel>
</emphasis>
(UCR 23700) in life. (C) Size differences between females and males. (D) Typical nocturnal pattern of males. (E) Female with the oocytes visible by transparency in the body. (F) Diurnal pattern of adults, males and females, note the white iris chance. (G) Juvenile. (H) Diurnal pattern with red-brown iris.
</paragraph>
</caption>
<caption id="39E78787D5534C5AFF27F863A158F805" ID-DOI="http://doi.org/10.5281/zenodo.7037406" ID-Zenodo-Dep="7037406" httpUri="https://zenodo.org/record/7037406/files/figure.png" pageId="9" pageNumber="510" startId="9.[152,255,1943,1967]" targetBox="[171,1415,193,1903]" targetPageId="9">
<paragraph id="6D27D70FD5534C5AFF27F863A158F805" blockId="9.[151,1436,1943,2040]" pageId="9" pageNumber="510">
<emphasis id="5FEC0B1DD5534C5AFF27F863A57DF84D" bold="true" box="[152,283,1943,1968]" pageId="9" pageNumber="510">FIGURE 5.</emphasis>
Variation and diagnostic characteristics in 
<taxonomicName id="AA98AC8CD5534C5AFD5DF86CA7CCF84D" authority="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack, 2022" authorityName="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack" authorityYear="2022" box="[738,938,1944,1968]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="9" pageNumber="510" phylum="Chordata" rank="species" species="celeste" status="sp. nov.">
<emphasis id="5FEC0B1DD5534C5AFD5DF86CA7CCF84D" box="[738,938,1944,1968]" italics="true" pageId="9" pageNumber="510">Tlalocohyla celeste</emphasis>
</taxonomicName>
<emphasis id="5FEC0B1DD5534C5AFC0CF86CA060F84D" bold="true" box="[947,1030,1944,1968]" pageId="9" pageNumber="510">
<taxonomicNameLabel id="44DFB666D5534C5AFC0CF86CA060F84D" box="[947,1030,1944,1968]" pageId="9" pageNumber="510" rank="species">sp. nov.</taxonomicNameLabel>
</emphasis>
(A-B) pattern change and green tones change of two uncollected males. (C) Ventral view in diurnal pattern. (D) ventral view of female (UCR 23701), note green bones (black arrow) and white peritoneum (blue arrow). (E) Spotted pattern in juvenile. (F) Axillary membrane (red arrow).
</paragraph>
</caption>
<caption id="39E78787D5504C59FF27F9A8A66AF941" ID-DOI="http://doi.org/10.5281/zenodo.7037410" ID-Zenodo-Dep="7037410" httpUri="https://zenodo.org/record/7037410/files/figure.png" pageId="10" pageNumber="511" startId="10.[152,255,1628,1652]" targetBox="[152,1435,181,1604]" targetPageId="10">
<paragraph id="6D27D70FD5504C59FF27F9A8A66AF941" blockId="10.[151,1437,1628,1725]" pageId="10" pageNumber="511">
<emphasis id="5FEC0B1DD5504C59FF27F9A8A57AF989" bold="true" box="[152,284,1628,1652]" pageId="10" pageNumber="511">FIGURE 6.</emphasis>
Ventral view 
<taxonomicName id="AA98AC8CD5504C59FE09F9A8A618F989" authority="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack, 2022" authorityName="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack" authorityYear="2022" box="[438,638,1628,1652]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="10" pageNumber="511" phylum="Chordata" rank="species" species="celeste" status="sp. nov.">
<emphasis id="5FEC0B1DD5504C59FE09F9A8A618F989" box="[438,638,1628,1652]" italics="true" pageId="10" pageNumber="511">Tlalocohyla celeste</emphasis>
</taxonomicName>
<emphasis id="5FEC0B1DD5504C59FD37F9A9A6BBF988" bold="true" box="[648,733,1629,1653]" pageId="10" pageNumber="511">
<taxonomicNameLabel id="44DFB666D5504C59FD37F9A9A6BBF988" box="[648,733,1629,1653]" pageId="10" pageNumber="511" rank="species">sp. nov.</taxonomicNameLabel>
</emphasis>
(A) Ventrals skin texture, and cloacal view. (B) Liver of recently preserved specimen (ECB-Anf-f50-08-01,01.). (C) Nuptial pads of the left hand in 
<taxonomicName id="AA98AC8CD5504C59FC57F974A0CAF965" authority="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack, 2022" authorityName="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack" authorityYear="2022" box="[1000,1196,1664,1688]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="10" pageNumber="511" phylum="Chordata" rank="species" species="celeste" status="sp. nov.">
<emphasis id="5FEC0B1DD5504C59FC57F974A0CAF965" box="[1000,1196,1664,1688]" italics="true" pageId="10" pageNumber="511">Tlalocohyla celeste</emphasis>
</taxonomicName>
<emphasis id="5FEC0B1DD5504C59FB0CF975A162F964" bold="true" box="[1203,1284,1665,1689]" pageId="10" pageNumber="511">
<taxonomicNameLabel id="44DFB666D5504C59FB0CF975A162F964" box="[1203,1284,1665,1689]" pageId="10" pageNumber="511" rank="species">sp. nov.</taxonomicNameLabel>
</emphasis>
male holotype (UCR 23700), in life, and preserved.
</paragraph>
</caption>
<paragraph id="6D27D70FD5504C59FF78F91FA78CF8D4" blockId="10.[151,1436,1770,2013]" pageId="10" pageNumber="511">
<emphasis id="5FEC0B1DD5504C59FF78F91FA6C6F8F9" bold="true" box="[199,672,1770,1797]" pageId="10" pageNumber="511">
Measurements of the 
<typeStatus id="B22369ADD5504C59FE75F91FA657F8F8" box="[458,561,1771,1797]" pageId="10" pageNumber="511" type="holotype">holotype</typeStatus>
(in mm).
</emphasis>
SVL 20.10; HL 7.70; HW 7.35; IND 1.89; IOD 3.26; ED 2.14; EN 1.88; TD 0.49; HAL 6.65; FL 8.75; TL 11.45; Fin3DW 0.55; Toe4DW 0.75.
</paragraph>
<paragraph id="6D27D70FD5504C58FF78F8C6A656FE50" blockId="10.[151,1436,1770,2013]" lastBlockId="11.[151,1437,151,429]" lastPageId="11" lastPageNumber="512" pageId="10" pageNumber="511">
<emphasis id="5FEC0B1DD5504C59FF78F8C6A70CF8B1" bold="true" box="[199,874,1842,1869]" pageId="10" pageNumber="511">
Coloration of the 
<typeStatus id="B22369ADD5504C59FE21F8C7A663F8B0" box="[414,517,1843,1869]" pageId="10" pageNumber="511" type="holotype">holotype</typeStatus>
in life (nocturnal coloration).
</emphasis>
Background color of head, upper back and limbs brilliant yellow-green (#103), with numerous mahogany red (#34) blotches relatively evenly dispersed over the dorsal surfaces of the head, body and lower limbs (
<figureCitation id="F5A3CB8AD5504C59FD52F88FA70DF868" box="[749,875,1915,1941]" captionStart="FIGURE 4" captionStartId="8.[152,255,1910,1934]" captionTargetBox="[172,1415,182,1885]" captionTargetId="figure-29@8.[172,1415,181,1886]" captionTargetPageId="8" captionText="FIGURE 4. Variation in dorsal pattern of Tlalocohyla celeste sp. nov. (A–B) male holotype of Tlalocohyla celeste sp. nov. (UCR 23700) in life. (C) Size differences between females and males. (D) Typical nocturnal pattern of males. (E) Female with the oocytes visible by transparency in the body. (F) Diurnal pattern of adults, males and females, note the white iris chance. (G) Juvenile. (H) Diurnal pattern with red-brown iris." figureDoi="http://doi.org/10.5281/zenodo.7037399" httpUri="https://zenodo.org/record/7037399/files/figure.png" pageId="10" pageNumber="511">Fig. 4A–B</figureCitation>
). A narrow sulfur white (#96) dorsolateral stripe starts behind each eye and extends towards the insertion of each hind limb, gradually fading out near a point about halfway down the body. A relatively poorly defined mahogany red stripe extends from the tip of the snout along the canthal region, and continues post-ocularly following the upper border of the sulfur white dorsolateral line; it gradually fades out at the same point as the light stripe. Lateral surfaces of the body and head, below the dorsolateral lines, lack mahogany red spots. Upper surfaces of finger and toe tips light greenish yellow (#87). Upper edge of orbit Pratt’s rufous (#72); remainder of upper eyelid suffused with spectrum yellow (#79). Posterior and anterior edge of orbit bordered by a narrow white line. Iris gold with raw umber (#23) reticulations. Vocal sac yellow-green (#103) when deflated, turquoise green (#147) when inflated. Skin covering ventral surfaces transparent, allowing muscles, veins, bones, and intestines to be visible (
<figureCitation id="F5A3CB8AD5514C58FD69FE9BA735FE74" box="[726,851,366,393]" captionStart="FIGURE 5" captionStartId="9.[152,255,1943,1967]" captionTargetBox="[171,1415,193,1903]" captionTargetId="figure-18@9.[152,1434,181,1920]" captionTargetPageId="9" captionText="FIGURE 5. Variation and diagnostic characteristics in Tlalocohyla celeste sp. nov. (A-B) pattern change and green tones change of two uncollected males. (C) Ventral view in diurnal pattern. (D) ventral view of female (UCR 23701), note green bones (black arrow) and white peritoneum (blue arrow). (E) Spotted pattern in juvenile. (F) Axillary membrane (red arrow)." figureDoi="http://doi.org/10.5281/zenodo.7037406" httpUri="https://zenodo.org/record/7037406/files/figure.png" pageId="11" pageNumber="512">Fig. 5C–D</figureCitation>
). Internal organs black, but hidden from view by an opaque, white peritoneum (
<figureCitation id="F5A3CB8AD5514C58FE75FE67A645FE50" box="[458,547,403,429]" captionStart="FIGURE 6" captionStartId="10.[152,255,1628,1652]" captionTargetBox="[152,1435,181,1604]" captionTargetId="figure-29@10.[151,1436,181,1604]" captionTargetPageId="10" captionText="FIGURE 6. Ventral view Tlalocohyla celeste sp. nov. (A) Ventrals skin texture, and cloacal view. (B) Liver of recently preserved specimen (ECB-Anf-f50-08-01,01.). (C) Nuptial pads of the left hand in Tlalocohyla celeste sp. nov. male holotype (UCR 23700), in life, and preserved." figureDoi="http://doi.org/10.5281/zenodo.7037410" httpUri="https://zenodo.org/record/7037410/files/figure.png" pageId="11" pageNumber="512">Fig. 6B</figureCitation>
).
</paragraph>
<caption id="39E78787D5514C58FF27F882A074F84F" ID-DOI="http://doi.org/10.5281/zenodo.7037412" ID-Zenodo-Dep="7037412" httpUri="https://zenodo.org/record/7037412/files/figure.png" pageId="11" pageNumber="512" startId="11.[152,255,1910,1934]" targetBox="[154,1435,456,1884]" targetPageId="11">
<paragraph id="6D27D70FD5514C58FF27F882A074F84F" blockId="11.[151,1437,1910,1970]" pageId="11" pageNumber="512">
<emphasis id="5FEC0B1DD5514C58FF27F882A57CF873" bold="true" box="[152,282,1910,1934]" pageId="11" pageNumber="512">FIGURE 7.</emphasis>
Vocal sac of uncollected males of 
<taxonomicName id="AA98AC8CD5514C58FD3AF882A72DF873" authority="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack, 2022" authorityName="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack" authorityYear="2022" box="[645,843,1910,1934]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="11" pageNumber="512" phylum="Chordata" rank="species" species="celeste" status="sp. nov.">
<emphasis id="5FEC0B1DD5514C58FD3AF882A72DF873" box="[645,843,1910,1934]" italics="true" pageId="11" pageNumber="512">Tlalocohyla celeste</emphasis>
</taxonomicName>
<emphasis id="5FEC0B1DD5514C58FCECF882A7C3F873" bold="true" box="[851,933,1910,1934]" pageId="11" pageNumber="512">
<taxonomicNameLabel id="44DFB666D5514C58FCECF882A7C3F873" box="[851,933,1910,1934]" pageId="11" pageNumber="512" rank="species">sp. nov.</taxonomicNameLabel>
</emphasis>
(A) Ventral view and vocal sac deflated. (B–D). Calling males perched on vegetation in different positions, note the vocal sac extension.
</paragraph>
</caption>
<paragraph id="6D27D70FD5564C5FFF78FF62A7A9FEBC" blockId="12.[151,1437,150,609]" pageId="12" pageNumber="513">
<emphasis id="5FEC0B1DD5564C5FFF78FF62A6DFFF4D" bold="true" box="[199,697,150,177]" pageId="12" pageNumber="513">
Coloration of the 
<typeStatus id="B22369ADD5564C5FFE2AFF63A59AFF4C" box="[405,508,151,177]" pageId="12" pageNumber="513" type="holotype">holotype</typeStatus>
in preservative.
</emphasis>
In formalin, yellow-green dorsal coloration becomes pale greenish white (#97), while mahogany red markings (dorsolateral lines and dorsal spots) turn pale vinaceous (#247). The color pattern fades in a similar fashion after ethanol preservation, leaving dorsolateral markings and dorsal spots faded but visible (
<figureCitation id="F5A3CB8AD5564C5FFEDEFEF7A5C1FEE0" box="[353,423,259,285]" captionStart="FIGURE 2" captionStartId="6.[152,255,1472,1496]" captionTargetBox="[152,1436,885,1449]" captionTargetId="figure-359@6.[152,1436,885,1449]" captionTargetPageId="6" captionText="FIGURE 2. Holotype of Tlalocohyla celeste sp. nov. (UCR 23700). (A) Dorsal view. (B) Ventral view." figureDoi="http://doi.org/10.5281/zenodo.7037395" httpUri="https://zenodo.org/record/7037395/files/figure.png" pageId="12" pageNumber="513">Fig. 2</figureCitation>
). The iris becomes black (
<figureCitation id="F5A3CB8AD5564C5FFD72FEF7A774FEE0" box="[717,786,259,285]" captionStart="FIGURE 3" captionStartId="7.[152,255,1932,1956]" captionTargetBox="[151,1435,309,1890]" captionTargetId="figure-84@7.[151,1436,309,1908]" captionTargetPageId="7" captionText="FIGURE 3. Holotype of Tlalocohyla celeste sp. nov. (UCR 23700) (A) Dorsal view of head in living specimen. (B) Lateral view of head in preserved specimen. (C) Lateral view in preserved specimen, note the tympanum. Scale bar = 2 mm. (D) Right hand in ventral view. (E) Right foot in ventral view." figureDoi="http://doi.org/10.5281/zenodo.7037397" httpUri="https://zenodo.org/record/7037397/files/figure.png" pageId="12" pageNumber="513">Fig. 3</figureCitation>
). Hands, feet, and tips of digits fade to a uniform creamywhite. Skin on ventral surfaces retains some transparency in preservative.
</paragraph>
<paragraph id="6D27D70FD5564C5FFF78FEBEA657FD9C" blockId="12.[151,1437,150,609]" pageId="12" pageNumber="513">
<emphasis id="5FEC0B1DD5564C5FFF78FEBEA559FE99" bold="true" box="[199,319,330,356]" pageId="12" pageNumber="513">Variation.</emphasis>
Morphometric variation is detailed in 
<tableCitation id="201AE2B4D5564C5FFD40FEBFA730FE98" box="[767,854,331,357]" captionStart="TABLE 1" captionStartId="3.[152,244,800,824]" captionTargetPageId="3" captionText="TABLE 1. Measurements (in mm) and ratios of the type series (including the holotype in bold) of Tlalocohyla celeste sp. nov. See Materials and methods section for the abbreviations of measurements." httpUri="http://table.plazi.org/id/39E78787D5594C50FF27FCD4A780FCA1" pageId="12" pageNumber="513" tableUuid="39E78787D5594C50FF27FCD4A780FCA1">Table 1</tableCitation>
. Females are larger than males (
<figureCitation id="F5A3CB8AD5564C5FFB6EFEBFA14FFE98" box="[1233,1321,331,357]" captionStart="FIGURE 4" captionStartId="8.[152,255,1910,1934]" captionTargetBox="[172,1415,182,1885]" captionTargetId="figure-29@8.[172,1415,181,1886]" captionTargetPageId="8" captionText="FIGURE 4. Variation in dorsal pattern of Tlalocohyla celeste sp. nov. (A–B) male holotype of Tlalocohyla celeste sp. nov. (UCR 23700) in life. (C) Size differences between females and males. (D) Typical nocturnal pattern of males. (E) Female with the oocytes visible by transparency in the body. (F) Diurnal pattern of adults, males and females, note the white iris chance. (G) Juvenile. (H) Diurnal pattern with red-brown iris." figureDoi="http://doi.org/10.5281/zenodo.7037399" httpUri="https://zenodo.org/record/7037399/files/figure.png" pageId="12" pageNumber="513">Fig. 4C</figureCitation>
) and lack nuptial pads, vocal slits and a vocal sac. The paratopotypes are comparable in coloration to the 
<typeStatus id="B22369ADD5564C5FFB00FE9BA143FE74" box="[1215,1317,367,393]" pageId="12" pageNumber="513" type="holotype">holotype</typeStatus>
. There is a noticeable difference between nocturnal (
<figureCitation id="F5A3CB8AD5564C5FFDD7FE67A6A6FE50" box="[616,704,403,429]" captionStart="FIGURE 4" captionStartId="8.[152,255,1910,1934]" captionTargetBox="[172,1415,182,1885]" captionTargetId="figure-29@8.[172,1415,181,1886]" captionTargetPageId="8" captionText="FIGURE 4. Variation in dorsal pattern of Tlalocohyla celeste sp. nov. (A–B) male holotype of Tlalocohyla celeste sp. nov. (UCR 23700) in life. (C) Size differences between females and males. (D) Typical nocturnal pattern of males. (E) Female with the oocytes visible by transparency in the body. (F) Diurnal pattern of adults, males and females, note the white iris chance. (G) Juvenile. (H) Diurnal pattern with red-brown iris." figureDoi="http://doi.org/10.5281/zenodo.7037399" httpUri="https://zenodo.org/record/7037399/files/figure.png" pageId="12" pageNumber="513">Fig. 4D</figureCitation>
) and diurnal coloration (
<figureCitation id="F5A3CB8AD5564C5FFC68FE67A04DFE50" box="[983,1067,403,429]" captionStart="FIGURE 4" captionStartId="8.[152,255,1910,1934]" captionTargetBox="[172,1415,182,1885]" captionTargetId="figure-29@8.[172,1415,181,1886]" captionTargetPageId="8" captionText="FIGURE 4. Variation in dorsal pattern of Tlalocohyla celeste sp. nov. (A–B) male holotype of Tlalocohyla celeste sp. nov. (UCR 23700) in life. (C) Size differences between females and males. (D) Typical nocturnal pattern of males. (E) Female with the oocytes visible by transparency in the body. (F) Diurnal pattern of adults, males and females, note the white iris chance. (G) Juvenile. (H) Diurnal pattern with red-brown iris." figureDoi="http://doi.org/10.5281/zenodo.7037399" httpUri="https://zenodo.org/record/7037399/files/figure.png" pageId="12" pageNumber="513">Fig. 4F</figureCitation>
) in this species, especially in the intensity and visibility of the mahogany red dorsal spots and dorsolateral lines. These markings are less prominent during the day, causing the dorsal pattern to appear more uniformly green and less spotted. In addition, the eye color brightens during the daytime to a lighter silver-gray (
<figureCitation id="F5A3CB8AD5564C5FFC92FE0BA7E5FDE4" box="[813,899,511,537]" captionStart="FIGURE 4" captionStartId="8.[152,255,1910,1934]" captionTargetBox="[172,1415,182,1885]" captionTargetId="figure-29@8.[172,1415,181,1886]" captionTargetPageId="8" captionText="FIGURE 4. Variation in dorsal pattern of Tlalocohyla celeste sp. nov. (A–B) male holotype of Tlalocohyla celeste sp. nov. (UCR 23700) in life. (C) Size differences between females and males. (D) Typical nocturnal pattern of males. (E) Female with the oocytes visible by transparency in the body. (F) Diurnal pattern of adults, males and females, note the white iris chance. (G) Juvenile. (H) Diurnal pattern with red-brown iris." figureDoi="http://doi.org/10.5281/zenodo.7037399" httpUri="https://zenodo.org/record/7037399/files/figure.png" pageId="12" pageNumber="513">Fig. 4F</figureCitation>
). The presence of blue green bones, lymph, and other tissues (
<figureCitation id="F5A3CB8AD5564C5FFE8FFDD7A514FDC0" box="[304,370,546,573]" captionStart="FIGURE 5" captionStartId="9.[152,255,1943,1967]" captionTargetBox="[171,1415,193,1903]" captionTargetId="figure-18@9.[152,1434,181,1920]" captionTargetPageId="9" captionText="FIGURE 5. Variation and diagnostic characteristics in Tlalocohyla celeste sp. nov. (A-B) pattern change and green tones change of two uncollected males. (C) Ventral view in diurnal pattern. (D) ventral view of female (UCR 23701), note green bones (black arrow) and white peritoneum (blue arrow). (E) Spotted pattern in juvenile. (F) Axillary membrane (red arrow)." figureDoi="http://doi.org/10.5281/zenodo.7037406" httpUri="https://zenodo.org/record/7037406/files/figure.png" pageId="12" pageNumber="513">Fig. 5</figureCitation>
) indicates that this species has physiological chlorosis, a trait that has been documented in other arboreal frogs (
<bibRefCitation id="0909AAFED5564C5FFEFDFDB3A643FD9C" author="Taboada, C. &amp; Brunetti, A. E. &amp; Lyra, M. L. &amp; Fitak, R. &amp; Faigon, S. A. &amp; Ron, S. &amp; Lagorio, M. G. &amp; Haddad, C. F. B. &amp; Lopes, N. P. &amp; Johnsen, S. &amp; Faivovich, J. &amp; Chemes, L. B. &amp; Bari, S. E." box="[322,549,583,609]" pageId="12" pageNumber="513" pagination="18574 - 18581" refId="ref14244" refString="Taboada, C., Brunetti, A. E., Lyra, M. L., Fitak, R. R, Faigon, S. A., Ron, S. R, Lagorio, M. G., Haddad, C. F. B., Lopes, N. P., Johnsen, S., Faivovich, J., Chemes, L. B. &amp; Bari, S. E. (2020) Multiple origins of green coloration in frogs mediated by a novel biliverdin-binding serpin. Proceedings of the National Academy of Sciences of the United States of America, 117 (31), 18574 - 18581." type="journal article" year="2020">
Taboada 
<emphasis id="5FEC0B1DD5564C5FFE16FDB3A587FD9C" box="[425,481,583,609]" italics="true" pageId="12" pageNumber="513">et al.</emphasis>
2020
</bibRefCitation>
).
</paragraph>
<caption id="39E78787D5564C5FFF27FD64A674FD31" ID-Table-UUID="39E78787D5564C5FFF27FD64A674FD31" httpUri="http://table.plazi.org/id/39E78787D5564C5FFF27FD64A674FD31" pageId="12" pageNumber="513" startId="12.[152,244,656,680]" targetBox="[166,1308,733,1562]" targetIsTable="true" targetPageId="12">
<paragraph id="6D27D70FD5564C5FFF27FD64A674FD31" blockId="12.[151,1436,656,716]" pageId="12" pageNumber="513">
<emphasis id="5FEC0B1DD5564C5FFF27FD64A568FD55" bold="true" box="[152,270,656,680]" pageId="12" pageNumber="513">TABLE 2.</emphasis>
Measurements (in mm) of the tadpoles of 
<taxonomicName id="AA98AC8CD5564C5FFD6BFD64A7C5FD55" authority="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack, 2022" authorityName="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack" authorityYear="2022" box="[724,931,656,680]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="12" pageNumber="513" phylum="Chordata" rank="species" species="celeste" status="sp. nov.">
<emphasis id="5FEC0B1DD5564C5FFD6BFD64A7C5FD55" box="[724,931,656,680]" italics="true" pageId="12" pageNumber="513">Tlalocohyla celeste</emphasis>
</taxonomicName>
<emphasis id="5FEC0B1DD5564C5FFC16FD65A799FD54" bold="true" box="[937,1023,657,681]" pageId="12" pageNumber="513">
<taxonomicNameLabel id="44DFB666D5564C5FFC16FD65A799FD54" box="[937,1023,657,681]" pageId="12" pageNumber="513" rank="species">sp. nov.</taxonomicNameLabel>
</emphasis>
See Materials and methods section for the abbreviations of measurements.
</paragraph>
</caption>
<paragraph id="6D27D70FD5564C5FFF19FD29A17AF9E4" pageId="12" pageNumber="513">
<table id="1F9825AFD556B3ACFF19FD29A17AF9E7" box="[166,1308,733,1562]" gridcols="4" gridrows="21" pageId="12" pageNumber="513">
<tr id="D3A8D54DD556B3ACFF19FD29A17AFD08" box="[166,1308,733,757]" gridrow="0" pageId="12" pageNumber="513" rowspan-3="1">
<th id="9079BC31D556B3ACFF19FD29A55CFD08" box="[166,314,733,757]" gridcol="0" gridrow="0" pageId="12" pageNumber="513">Measurements</th>
<th id="9079BC31D556B3ACFC32FD29A00CFD08" box="[909,1130,733,757]" colspan="2" colspanRight="1" gridcol="1" gridrow="0" pageId="12" pageNumber="513">Stages 33-34 (N = 3)</th>
</tr>
<tr id="D3A8D54DD556B3ACFF19FCF0A17AFCE1" box="[166,1308,772,796]" gridrow="1" pageId="12" pageNumber="513" rowspan-0="1">
<td id="9079BC31D556B3ACFC32FCF0A7DFFCE1" box="[909,953,772,796]" gridcol="1" gridrow="1" pageId="12" pageNumber="513">
<emphasis id="5FEC0B1DD5564C5FFC32FCF0A7FCFCE1" bold="true" box="[909,922,772,796]" pageId="12" pageNumber="513">1</emphasis>
</td>
<td id="9079BC31D556B3ACFB81FCF0A00CFCE1" box="[1086,1130,772,796]" gridcol="2" gridrow="1" pageId="12" pageNumber="513">
<emphasis id="5FEC0B1DD5564C5FFB81FCF0A02DFCE1" bold="true" box="[1086,1099,772,796]" pageId="12" pageNumber="513">2</emphasis>
</td>
<td id="9079BC31D556B3ACFB4FFCF0A17AFCE1" box="[1264,1308,772,796]" gridcol="3" gridrow="1" pageId="12" pageNumber="513">
<emphasis id="5FEC0B1DD5564C5FFB4FFCF0A09BFCE1" bold="true" box="[1264,1277,772,796]" pageId="12" pageNumber="513">3</emphasis>
</td>
</tr>
<tr id="D3A8D54DD556B3ACFF19FCD9A17AFCB8" box="[166,1308,813,837]" gridrow="2" pageId="12" pageNumber="513">
<th id="9079BC31D556B3ACFF19FCD9A55CFCB8" box="[166,314,813,837]" gridcol="0" gridrow="2" pageId="12" pageNumber="513">TL</th>
<td id="9079BC31D556B3ACFC32FCD9A7DFFCB8" box="[909,953,813,837]" gridcol="1" gridrow="2" pageId="12" pageNumber="513">26.6</td>
<td id="9079BC31D556B3ACFB81FCD9A00CFCB8" box="[1086,1130,813,837]" gridcol="2" gridrow="2" pageId="12" pageNumber="513">26.1</td>
<td id="9079BC31D556B3ACFB4FFCD9A17AFCB8" box="[1264,1308,813,837]" gridcol="3" gridrow="2" pageId="12" pageNumber="513">29.7</td>
</tr>
<tr id="D3A8D54DD556B3ACFF19FCA1A17AFC93" box="[166,1308,853,878]" gridrow="3" pageId="12" pageNumber="513">
<th id="9079BC31D556B3ACFF19FCA1A55CFC93" box="[166,314,853,878]" gridcol="0" gridrow="3" pageId="12" pageNumber="513">BL</th>
<td id="9079BC31D556B3ACFC32FCA1A7DFFC93" box="[909,953,853,878]" gridcol="1" gridrow="3" pageId="12" pageNumber="513">10.6</td>
<td id="9079BC31D556B3ACFB81FCA1A00CFC93" box="[1086,1130,853,878]" gridcol="2" gridrow="3" pageId="12" pageNumber="513">9.4</td>
<td id="9079BC31D556B3ACFB4FFCA1A17AFC93" box="[1264,1308,853,878]" gridcol="3" gridrow="3" pageId="12" pageNumber="513">10.3</td>
</tr>
<tr id="D3A8D54DD556B3ACFF19FC8AA17AFC6B" box="[166,1308,894,918]" gridrow="4" pageId="12" pageNumber="513">
<th id="9079BC31D556B3ACFF19FC8AA55CFC6B" box="[166,314,894,918]" gridcol="0" gridrow="4" pageId="12" pageNumber="513">TAL</th>
<td id="9079BC31D556B3ACFC32FC8AA7DFFC6B" box="[909,953,894,918]" gridcol="1" gridrow="4" pageId="12" pageNumber="513">14.9</td>
<td id="9079BC31D556B3ACFB81FC8AA00CFC6B" box="[1086,1130,894,918]" gridcol="2" gridrow="4" pageId="12" pageNumber="513">20.8</td>
<td id="9079BC31D556B3ACFB4FFC8AA17AFC6B" box="[1264,1308,894,918]" gridcol="3" gridrow="4" pageId="12" pageNumber="513">21.0</td>
</tr>
<tr id="D3A8D54DD556B3ACFF19FC52A17AFC43" box="[166,1308,934,958]" gridrow="5" pageId="12" pageNumber="513">
<th id="9079BC31D556B3ACFF19FC52A55CFC43" box="[166,314,934,958]" gridcol="0" gridrow="5" pageId="12" pageNumber="513">MTH</th>
<td id="9079BC31D556B3ACFC32FC52A7DFFC43" box="[909,953,934,958]" gridcol="1" gridrow="5" pageId="12" pageNumber="513">5.68</td>
<td id="9079BC31D556B3ACFB81FC52A00CFC43" box="[1086,1130,934,958]" gridcol="2" gridrow="5" pageId="12" pageNumber="513">4.53</td>
<td id="9079BC31D556B3ACFB4FFC52A17AFC43" box="[1264,1308,934,958]" gridcol="3" gridrow="5" pageId="12" pageNumber="513">5.61</td>
</tr>
<tr id="D3A8D54DD556B3ACFF19FC3AA17AFC1B" box="[166,1308,974,998]" gridrow="6" pageId="12" pageNumber="513">
<th id="9079BC31D556B3ACFF19FC3AA55CFC1B" box="[166,314,974,998]" gridcol="0" gridrow="6" pageId="12" pageNumber="513">TMH</th>
<td id="9079BC31D556B3ACFC32FC3AA7DFFC1B" box="[909,953,974,998]" gridcol="1" gridrow="6" pageId="12" pageNumber="513">2.93</td>
<td id="9079BC31D556B3ACFB81FC3AA00CFC1B" box="[1086,1130,974,998]" gridcol="2" gridrow="6" pageId="12" pageNumber="513">2.92</td>
<td id="9079BC31D556B3ACFB4FFC3AA17AFC1B" box="[1264,1308,974,998]" gridcol="3" gridrow="6" pageId="12" pageNumber="513">2.55</td>
</tr>
<tr id="D3A8D54DD556B3ACFF19FC02A17AFBF3" box="[166,1308,1014,1038]" gridrow="7" pageId="12" pageNumber="513">
<th id="9079BC31D556B3ACFF19FC02A55CFBF3" box="[166,314,1014,1038]" gridcol="0" gridrow="7" pageId="12" pageNumber="513">TMW</th>
<td id="9079BC31D556B3ACFC32FC02A7DFFBF3" box="[909,953,1014,1038]" gridcol="1" gridrow="7" pageId="12" pageNumber="513">2.63</td>
<td id="9079BC31D556B3ACFB81FC02A00CFBF3" box="[1086,1130,1014,1038]" gridcol="2" gridrow="7" pageId="12" pageNumber="513">2.85</td>
<td id="9079BC31D556B3ACFB4FFC02A17AFBF3" box="[1264,1308,1014,1038]" gridcol="3" gridrow="7" pageId="12" pageNumber="513">1.89</td>
</tr>
<tr id="D3A8D54DD556B3ACFF19FBEAA17AFBCB" box="[166,1308,1054,1078]" gridrow="8" pageId="12" pageNumber="513">
<th id="9079BC31D556B3ACFF19FBEAA55CFBCB" box="[166,314,1054,1078]" gridcol="0" gridrow="8" pageId="12" pageNumber="513">BH</th>
<td id="9079BC31D556B3ACFC32FBEAA7DFFBCB" box="[909,953,1054,1078]" gridcol="1" gridrow="8" pageId="12" pageNumber="513">5.50</td>
<td id="9079BC31D556B3ACFB81FBEAA00CFBCB" box="[1086,1130,1054,1078]" gridcol="2" gridrow="8" pageId="12" pageNumber="513">5.59</td>
<td id="9079BC31D556B3ACFB4FFBEAA17AFBCB" box="[1264,1308,1054,1078]" gridcol="3" gridrow="8" pageId="12" pageNumber="513">5.09</td>
</tr>
<tr id="D3A8D54DD556B3ACFF19FBB3A17AFBA2" box="[166,1308,1095,1119]" gridrow="9" pageId="12" pageNumber="513">
<th id="9079BC31D556B3ACFF19FBB3A55CFBA2" box="[166,314,1095,1119]" gridcol="0" gridrow="9" pageId="12" pageNumber="513">BW</th>
<td id="9079BC31D556B3ACFC32FBB3A7DFFBA2" box="[909,953,1095,1119]" gridcol="1" gridrow="9" pageId="12" pageNumber="513">6.54</td>
<td id="9079BC31D556B3ACFB81FBB3A00CFBA2" box="[1086,1130,1095,1119]" gridcol="2" gridrow="9" pageId="12" pageNumber="513">6.51</td>
<td id="9079BC31D556B3ACFB4FFBB3A17AFBA2" box="[1264,1308,1095,1119]" gridcol="3" gridrow="9" pageId="12" pageNumber="513">5.42</td>
</tr>
<tr id="D3A8D54DD556B3ACFF19FB9BA17AFB7A" box="[166,1308,1135,1159]" gridrow="10" pageId="12" pageNumber="513">
<th id="9079BC31D556B3ACFF19FB9BA55CFB7A" box="[166,314,1135,1159]" gridcol="0" gridrow="10" pageId="12" pageNumber="513">ED</th>
<td id="9079BC31D556B3ACFC32FB9BA7DFFB7A" box="[909,953,1135,1159]" gridcol="1" gridrow="10" pageId="12" pageNumber="513">1.44</td>
<td id="9079BC31D556B3ACFB81FB9BA00CFB7A" box="[1086,1130,1135,1159]" gridcol="2" gridrow="10" pageId="12" pageNumber="513">1.47</td>
<td id="9079BC31D556B3ACFB4FFB9BA17AFB7A" box="[1264,1308,1135,1159]" gridcol="3" gridrow="10" pageId="12" pageNumber="513">1.11</td>
</tr>
<tr id="D3A8D54DD556B3ACFF19FB63A17AFB52" box="[166,1308,1175,1199]" gridrow="11" pageId="12" pageNumber="513">
<th id="9079BC31D556B3ACFF19FB63A55CFB52" box="[166,314,1175,1199]" gridcol="0" gridrow="11" pageId="12" pageNumber="513">ODW</th>
<td id="9079BC31D556B3ACFC32FB63A7DFFB52" box="[909,953,1175,1199]" gridcol="1" gridrow="11" pageId="12" pageNumber="513">2.99</td>
<td id="9079BC31D556B3ACFB81FB63A00CFB52" box="[1086,1130,1175,1199]" gridcol="2" gridrow="11" pageId="12" pageNumber="513">2.54</td>
<td id="9079BC31D556B3ACFB4FFB63A17AFB52" box="[1264,1308,1175,1199]" gridcol="3" gridrow="11" pageId="12" pageNumber="513">2.31</td>
</tr>
<tr id="D3A8D54DD556B3ACFF19FB4BA17AFB25" box="[166,1308,1215,1240]" gridrow="12" pageId="12" pageNumber="513">
<th id="9079BC31D556B3ACFF19FB4BA55CFB25" box="[166,314,1215,1240]" gridcol="0" gridrow="12" pageId="12" pageNumber="513">END</th>
<td id="9079BC31D556B3ACFC32FB4BA7DFFB25" box="[909,953,1215,1240]" gridcol="1" gridrow="12" pageId="12" pageNumber="513">2.07</td>
<td id="9079BC31D556B3ACFB81FB4BA00CFB25" box="[1086,1130,1215,1240]" gridcol="2" gridrow="12" pageId="12" pageNumber="513">1.95</td>
<td id="9079BC31D556B3ACFB4FFB4BA17AFB25" box="[1264,1308,1215,1240]" gridcol="3" gridrow="12" pageId="12" pageNumber="513">1.68</td>
</tr>
<tr id="D3A8D54DD556B3ACFF19FB1CA17AFAFD" box="[166,1308,1256,1280]" gridrow="13" pageId="12" pageNumber="513">
<th id="9079BC31D556B3ACFF19FB1CA55CFAFD" box="[166,314,1256,1280]" gridcol="0" gridrow="13" pageId="12" pageNumber="513">NSD</th>
<td id="9079BC31D556B3ACFC32FB1CA7DFFAFD" box="[909,953,1256,1280]" gridcol="1" gridrow="13" pageId="12" pageNumber="513">1.33</td>
<td id="9079BC31D556B3ACFB81FB1CA00CFAFD" box="[1086,1130,1256,1280]" gridcol="2" gridrow="13" pageId="12" pageNumber="513">1.85</td>
<td id="9079BC31D556B3ACFB4FFB1CA17AFAFD" box="[1264,1308,1256,1280]" gridcol="3" gridrow="13" pageId="12" pageNumber="513">1.34</td>
</tr>
<tr id="D3A8D54DD556B3ACFF19FAE4A17AFAD5" box="[166,1308,1296,1320]" gridrow="14" pageId="12" pageNumber="513">
<th id="9079BC31D556B3ACFF19FAE4A55CFAD5" box="[166,314,1296,1320]" gridcol="0" gridrow="14" pageId="12" pageNumber="513">ND</th>
<td id="9079BC31D556B3ACFC32FAE4A7DFFAD5" box="[909,953,1296,1320]" gridcol="1" gridrow="14" pageId="12" pageNumber="513">0.54</td>
<td id="9079BC31D556B3ACFB81FAE4A00CFAD5" box="[1086,1130,1296,1320]" gridcol="2" gridrow="14" pageId="12" pageNumber="513">0.32</td>
<td id="9079BC31D556B3ACFB4FFAE4A17AFAD5" box="[1264,1308,1296,1320]" gridcol="3" gridrow="14" pageId="12" pageNumber="513">0.43</td>
</tr>
<tr id="D3A8D54DD556B3ACFF19FACCA17AFAAD" box="[166,1308,1336,1360]" gridrow="15" pageId="12" pageNumber="513">
<th id="9079BC31D556B3ACFF19FACCA55CFAAD" box="[166,314,1336,1360]" gridcol="0" gridrow="15" pageId="12" pageNumber="513">IND</th>
<td id="9079BC31D556B3ACFC32FACCA7DFFAAD" box="[909,953,1336,1360]" gridcol="1" gridrow="15" pageId="12" pageNumber="513">2.59</td>
<td id="9079BC31D556B3ACFB81FACCA00CFAAD" box="[1086,1130,1336,1360]" gridcol="2" gridrow="15" pageId="12" pageNumber="513">2.06</td>
<td id="9079BC31D556B3ACFB4FFACCA17AFAAD" box="[1264,1308,1336,1360]" gridcol="3" gridrow="15" pageId="12" pageNumber="513">1.95</td>
</tr>
<tr id="D3A8D54DD556B3ACFF19FA94A17AFA84" box="[166,1308,1376,1401]" gridrow="16" pageId="12" pageNumber="513">
<th id="9079BC31D556B3ACFF19FA94A55CFA84" box="[166,314,1376,1401]" gridcol="0" gridrow="16" pageId="12" pageNumber="513">IOD</th>
<td id="9079BC31D556B3ACFC32FA94A7DFFA84" box="[909,953,1376,1401]" gridcol="1" gridrow="16" pageId="12" pageNumber="513">4.47</td>
<td id="9079BC31D556B3ACFB81FA94A00CFA84" box="[1086,1130,1376,1401]" gridcol="2" gridrow="16" pageId="12" pageNumber="513">4.11</td>
<td id="9079BC31D556B3ACFB4FFA94A17AFA84" box="[1264,1308,1376,1401]" gridcol="3" gridrow="16" pageId="12" pageNumber="513">4.55</td>
</tr>
<tr id="D3A8D54DD556B3ACFF19FA7DA17AFA5C" box="[166,1308,1417,1441]" gridrow="17" pageId="12" pageNumber="513">
<th id="9079BC31D556B3ACFF19FA7DA55CFA5C" box="[166,314,1417,1441]" gridcol="0" gridrow="17" pageId="12" pageNumber="513">DFH</th>
<td id="9079BC31D556B3ACFC32FA7DA7DFFA5C" box="[909,953,1417,1441]" gridcol="1" gridrow="17" pageId="12" pageNumber="513">1.77</td>
<td id="9079BC31D556B3ACFB81FA7DA00CFA5C" box="[1086,1130,1417,1441]" gridcol="2" gridrow="17" pageId="12" pageNumber="513">1.47</td>
<td id="9079BC31D556B3ACFB4FFA7DA17AFA5C" box="[1264,1308,1417,1441]" gridcol="3" gridrow="17" pageId="12" pageNumber="513">2.24</td>
</tr>
<tr id="D3A8D54DD556B3ACFF19FA45A17AFA34" box="[166,1308,1457,1481]" gridrow="18" pageId="12" pageNumber="513">
<th id="9079BC31D556B3ACFF19FA45A55CFA34" box="[166,314,1457,1481]" gridcol="0" gridrow="18" pageId="12" pageNumber="513">VFH</th>
<td id="9079BC31D556B3ACFC32FA45A7DFFA34" box="[909,953,1457,1481]" gridcol="1" gridrow="18" pageId="12" pageNumber="513">1.47</td>
<td id="9079BC31D556B3ACFB81FA45A00CFA34" box="[1086,1130,1457,1481]" gridcol="2" gridrow="18" pageId="12" pageNumber="513">1.06</td>
<td id="9079BC31D556B3ACFB4FFA45A17AFA34" box="[1264,1308,1457,1481]" gridcol="3" gridrow="18" pageId="12" pageNumber="513">1.44</td>
</tr>
<tr id="D3A8D54DD556B3ACFF19FA2DA17AFA0C" box="[166,1308,1497,1521]" gridrow="19" pageId="12" pageNumber="513">
<th id="9079BC31D556B3ACFF19FA2DA55CFA0C" box="[166,314,1497,1521]" gridcol="0" gridrow="19" pageId="12" pageNumber="513">SL</th>
<td id="9079BC31D556B3ACFC32FA2DA7DFFA0C" box="[909,953,1497,1521]" gridcol="1" gridrow="19" pageId="12" pageNumber="513">1.56</td>
<td id="9079BC31D556B3ACFB81FA2DA00CFA0C" box="[1086,1130,1497,1521]" gridcol="2" gridrow="19" pageId="12" pageNumber="513">1.50</td>
<td id="9079BC31D556B3ACFB4FFA2DA17AFA0C" box="[1264,1308,1497,1521]" gridcol="3" gridrow="19" pageId="12" pageNumber="513">1.36</td>
</tr>
<tr id="D3A8D54DD556B3ACFF19F9F5A17AF9E7" box="[166,1308,1537,1562]" gridrow="20" pageId="12" pageNumber="513">
<th id="9079BC31D556B3ACFF19F9F5A55CF9E7" box="[166,314,1537,1562]" gridcol="0" gridrow="20" pageId="12" pageNumber="513">SS</th>
<td id="9079BC31D556B3ACFC32F9F5A7DFF9E7" box="[909,953,1537,1562]" gridcol="1" gridrow="20" pageId="12" pageNumber="513">6.44</td>
<td id="9079BC31D556B3ACFB81F9F5A00CF9E7" box="[1086,1130,1537,1562]" gridcol="2" gridrow="20" pageId="12" pageNumber="513">6.13</td>
<td id="9079BC31D556B3ACFB4FF9F5A17AF9E7" box="[1264,1308,1537,1562]" gridcol="3" gridrow="20" pageId="12" pageNumber="513">5.15</td>
</tr>
</table>
</paragraph>
<paragraph id="6D27D70FD5564C5FFF78F9B9A031F8C2" blockId="12.[151,1437,1613,2036]" pageId="12" pageNumber="513">
Metamorphs and small juveniles exhibit a lighter green dorsum and lack the light dorsolateral stripes during early developmental stages (
<figureCitation id="F5A3CB8AD5564C5FFE57F985A613F976" box="[488,629,1649,1675]" captionStart="FIGURE 11" captionStartId="17.[152,255,1972,1996]" captionTargetBox="[159,1429,630,1948]" captionTargetId="figure-229@17.[159,1429,630,1948]" captionTargetPageId="17" captionText="FIGURE 11. Metamorphosis of Tlalocohyla celeste sp. nov. (A) Tadpole at stage 35. (B) Metamorph of 11 mm of SVL. (C) Recently metamorphosed juvenile of 7 mm. (D) Juvenile." figureDoi="http://doi.org/10.5281/zenodo.7037420" httpUri="https://zenodo.org/record/7037420/files/figure.png" pageId="12" pageNumber="513">Fig. 11B–C</figureCitation>
). The small, light cyan axillary membrane (
<figureCitation id="F5A3CB8AD5564C5FFB37F985A087F971" box="[1160,1249,1649,1676]" captionStart="FIGURE 5" captionStartId="9.[152,255,1943,1967]" captionTargetBox="[171,1415,193,1903]" captionTargetId="figure-18@9.[152,1434,181,1920]" captionTargetPageId="9" captionText="FIGURE 5. Variation and diagnostic characteristics in Tlalocohyla celeste sp. nov. (A-B) pattern change and green tones change of two uncollected males. (C) Ventral view in diurnal pattern. (D) ventral view of female (UCR 23701), note green bones (black arrow) and white peritoneum (blue arrow). (E) Spotted pattern in juvenile. (F) Axillary membrane (red arrow)." figureDoi="http://doi.org/10.5281/zenodo.7037406" httpUri="https://zenodo.org/record/7037406/files/figure.png" pageId="12" pageNumber="513">Fig. 5F</figureCitation>
) is not seen in juveniles. Such young individuals generally display clusters of mahogany red blotches on the dorsum, most densely concentrated anteriorly but with considerable variation in their aggregation patterns. A broad, dark canthal stripe and short postocular stripe are usually present in juveniles, while the dorsal surfaces of the head, body, and limbs are marked with bold dark brown to purplish-brown spots. Dark-colored spots and stripes lighten ontogenetically likely to transform into the adult color pattern as the frogs age (
<figureCitation id="F5A3CB8AD5564C5FFCE2F8D1A7D0F8C2" box="[861,950,1829,1855]" captionStart="FIGURE 4" captionStartId="8.[152,255,1910,1934]" captionTargetBox="[172,1415,182,1885]" captionTargetId="figure-29@8.[172,1415,181,1886]" captionTargetPageId="8" captionText="FIGURE 4. Variation in dorsal pattern of Tlalocohyla celeste sp. nov. (A–B) male holotype of Tlalocohyla celeste sp. nov. (UCR 23700) in life. (C) Size differences between females and males. (D) Typical nocturnal pattern of males. (E) Female with the oocytes visible by transparency in the body. (F) Diurnal pattern of adults, males and females, note the white iris chance. (G) Juvenile. (H) Diurnal pattern with red-brown iris." figureDoi="http://doi.org/10.5281/zenodo.7037399" httpUri="https://zenodo.org/record/7037399/files/figure.png" pageId="12" pageNumber="513">Fig. 4G</figureCitation>
, 
<figureCitation id="F5A3CB8AD5564C5FFC7DF8D1A786F8BD" box="[962,992,1829,1856]" captionStart="FIGURE 5" captionStartId="9.[152,255,1943,1967]" captionTargetBox="[171,1415,193,1903]" captionTargetId="figure-18@9.[152,1434,181,1920]" captionTargetPageId="9" captionText="FIGURE 5. Variation and diagnostic characteristics in Tlalocohyla celeste sp. nov. (A-B) pattern change and green tones change of two uncollected males. (C) Ventral view in diurnal pattern. (D) ventral view of female (UCR 23701), note green bones (black arrow) and white peritoneum (blue arrow). (E) Spotted pattern in juvenile. (F) Axillary membrane (red arrow)." figureDoi="http://doi.org/10.5281/zenodo.7037406" httpUri="https://zenodo.org/record/7037406/files/figure.png" pageId="12" pageNumber="513">5E</figureCitation>
and 
<figureCitation id="F5A3CB8AD5564C5FFBA8F8D1A02FF8C2" box="[1047,1097,1829,1855]" captionStart="FIGURE 11" captionStartId="17.[152,255,1972,1996]" captionTargetBox="[159,1429,630,1948]" captionTargetId="figure-229@17.[159,1429,630,1948]" captionTargetPageId="17" captionText="FIGURE 11. Metamorphosis of Tlalocohyla celeste sp. nov. (A) Tadpole at stage 35. (B) Metamorph of 11 mm of SVL. (C) Recently metamorphosed juvenile of 7 mm. (D) Juvenile." figureDoi="http://doi.org/10.5281/zenodo.7037420" httpUri="https://zenodo.org/record/7037420/files/figure.png" pageId="12" pageNumber="513">11D</figureCitation>
).
</paragraph>
<paragraph id="6D27D70FD5564C5EFF78F8BDA637FEE0" blockId="12.[151,1437,1613,2036]" lastBlockId="13.[151,1437,151,1977]" lastPageId="13" lastPageNumber="514" pageId="12" pageNumber="513">
<emphasis id="5FEC0B1DD5564C5FFF78F8BDA5DDF89E" bold="true" box="[199,443,1865,1891]" pageId="12" pageNumber="513">Tadpole description.</emphasis>
Tadpoles of 
<taxonomicName id="AA98AC8CD5564C5FFDF3F8BDA743F89E" authorityName="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack" authorityYear="2022" box="[588,805,1865,1891]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="12" pageNumber="513" phylum="Chordata" rank="species" species="celeste">
<emphasis id="5FEC0B1DD5564C5FFDF3F8BDA743F89E" box="[588,805,1865,1891]" italics="true" pageId="12" pageNumber="513">Tlalocohyla celeste</emphasis>
</taxonomicName>
(
<figureCitation id="F5A3CB8AD5564C5FFC8AF8BDA7F1F89E" box="[821,919,1865,1891]" captionStart="FIGURE 11" captionStartId="17.[152,255,1972,1996]" captionTargetBox="[159,1429,630,1948]" captionTargetId="figure-229@17.[159,1429,630,1948]" captionTargetPageId="17" captionText="FIGURE 11. Metamorphosis of Tlalocohyla celeste sp. nov. (A) Tadpole at stage 35. (B) Metamorph of 11 mm of SVL. (C) Recently metamorphosed juvenile of 7 mm. (D) Juvenile." figureDoi="http://doi.org/10.5281/zenodo.7037420" httpUri="https://zenodo.org/record/7037420/files/figure.png" pageId="12" pageNumber="513">Fig. 11A</figureCitation>
and 
<figureCitation id="F5A3CB8AD5564C5FFC70F8BDA079F89E" box="[975,1055,1865,1891]" captionStart="FIGURE 12" captionStartId="18.[152,255,1943,1967]" captionTargetBox="[205,1379,199,1905]" captionTargetId="figure-29@18.[188,1400,181,1920]" captionTargetPageId="18" captionText="FIGURE 12. Tlalocohyla celeste sp. nov. tadpole at stage 34 (35) according to Gosner (1960). (A) Lateral view, (B) dorsal view, (C) ventral view, (D) oral disc." figureDoi="http://doi.org/10.5281/zenodo.7037423" httpUri="https://zenodo.org/record/7037423/files/figure.png" pageId="12" pageNumber="513">Fig. 12</figureCitation>
) in Gosner stages 33–44 conform to the following description: body depressed (BH/BW=0.87–0.94), ovoid in dorsal view and ovoid or elliptical in lateral view, from 0.35-0.40 times the TL. Snout rounded in dorsal and lateral aspects. Eyes medium-sized (ED/ BL=0.11–0.16), laterally located, visible in ventral view. The nostrils are situated approximately midway between the eyes and the tip of the snout. Nares rounded, medium-sized (ND/BL=0.03–0.05), dorsally located and dorsally directed. Well-developed fleshy flanges on the marginal rim. Spiracle sinistral, lateral, directed posterodorsally, and located on the posterior third of the body. Spiracle opening small (SL/BL=0.13–0.16). The intestinal mass is positioned perpendicular to the longitudinal body axis and visible through the clear ventral skin. The cloacal tube is short, dextral and has a triangular apex.
</paragraph>
<paragraph id="6D27D70FD5574C5EFF78FED3A0D7FD78" blockId="13.[151,1437,151,1977]" pageId="13" pageNumber="514">
The small oral disc (ODW/BW=0.39–0.46) is positioned anteroventrally. Oral disc not emarginated; has a single row of short marginal papillae with a small anterior gap. Several submarginal papillae are randomly distributed laterally in the oral disc (
<figureCitation id="F5A3CB8AD5574C5EFE18FE9BA669FE74" box="[423,527,367,393]" captionStart="FIGURE 12" captionStartId="18.[152,255,1943,1967]" captionTargetBox="[205,1379,199,1905]" captionTargetId="figure-29@18.[188,1400,181,1920]" captionTargetPageId="18" captionText="FIGURE 12. Tlalocohyla celeste sp. nov. tadpole at stage 34 (35) according to Gosner (1960). (A) Lateral view, (B) dorsal view, (C) ventral view, (D) oral disc." figureDoi="http://doi.org/10.5281/zenodo.7037423" httpUri="https://zenodo.org/record/7037423/files/figure.png" pageId="13" pageNumber="514">Fig. 12D</figureCitation>
). LTRF 2(2)/3; A-1 and A-2 of equal size; A-2 with a narrow medial gap; P-1 equal in size to P-2; P-3 approximately one quarter the size of P-1 and P-2. Jaw sheaths small, measuring less than half of the oral disc; finely serrated along their entire margin. Anterior jaw sheath smoothly arched, posterior jaw sheath shallowly V-shaped. Tail of intermediate height (MTH/TAL=0.21-0.38) with moderately robust musculature (TMH/ BH=0.50-0.53); dorsal fin higher than ventral fin (DFH/VFH=1.20-1.56). Dorsal and ventral fins of intermediate height (DFH/TAL=0.07-0.11; VFH/TAL=0.05-0.09); dorsal fin initiates on the body at a point slightly anterior to the base of the tail; maximum fin height is achieved near the midpoint of the tail’s length. Ventral fin originates towards the end of the venter, anterior to the cloacal tube. The lateral line system is indistinct.
</paragraph>
<paragraph id="6D27D70FD5574C5EFF78FD7BA6E9FB38" blockId="13.[151,1437,151,1977]" pageId="13" pageNumber="514">
In life, the general color pattern of the body and tail musculature is pale cream, pale white or pale yellow, with small brown flecks on the dorsal surfaces (
<figureCitation id="F5A3CB8AD5574C5EFDCDFD47A6B1FD30" box="[626,727,691,717]" captionStart="FIGURE 11" captionStartId="17.[152,255,1972,1996]" captionTargetBox="[159,1429,630,1948]" captionTargetId="figure-229@17.[159,1429,630,1948]" captionTargetPageId="17" captionText="FIGURE 11. Metamorphosis of Tlalocohyla celeste sp. nov. (A) Tadpole at stage 35. (B) Metamorph of 11 mm of SVL. (C) Recently metamorphosed juvenile of 7 mm. (D) Juvenile." figureDoi="http://doi.org/10.5281/zenodo.7037420" httpUri="https://zenodo.org/record/7037420/files/figure.png" pageId="13" pageNumber="514">Fig. 11A</figureCitation>
– 
<figureCitation id="F5A3CB8AD5574C5EFD58FD47A765FD30" box="[743,771,691,717]" captionStart="FIGURE 12" captionStartId="18.[152,255,1943,1967]" captionTargetBox="[205,1379,199,1905]" captionTargetId="figure-29@18.[188,1400,181,1920]" captionTargetPageId="18" captionText="FIGURE 12. Tlalocohyla celeste sp. nov. tadpole at stage 34 (35) according to Gosner (1960). (A) Lateral view, (B) dorsal view, (C) ventral view, (D) oral disc." figureDoi="http://doi.org/10.5281/zenodo.7037423" httpUri="https://zenodo.org/record/7037423/files/figure.png" pageId="13" pageNumber="514">12</figureCitation>
). Dorsal surfaces of head yellow ochre (#14) to tawny olive (#17); lateral surfaces slightly lighter in hue, marked with concentrations of mahogany red (#34) to warm sepia (#40) spots. A pattern of relatively evenly-spaced, rounded warm sepia (#40) spots is a prominent feature of the dorsal surfaces; dorsal and lateral surfaces also display a fine dusting of irregular white or gold specks. A distinct, but poorly defined warm sepia (#40) stripe extends from a point near the anterior edge of the oral disc towards the eye and continues as an indistinct postocular stripe onto the body. Iris gold, marked on either side of the pupil with a warm sepia (#40) spot; both dark spots align with the dark eye stripe that originates at the snout tip. Ventral skin is transparent, revealing reddish hues anteriorly at the thoracic level and cream white (#52) coloration at the level of the intestines. Throughout the larval development period, the intestinal tract becomes increasingly suffused with black pigment. This ontogenetic change creates a striking black-and-white ventral pattern that contrasts sharply with the dorsal and lateral coloration. Tail fins are transparent, marked with black or raw umber (#23) blotches of irregular shape and size, as well as small white spots. Tail musculature is yellow ochre (#14) with black or warm sepia (#40) spots, occasionally with a fine black line on the medial side of the tail that can be intact or interrupted. Upon preservation in 10% formalin, the larval coloration fades to pale greenish white (#97), whereas the eye stripe, dorsal spots and tail markings become black.
</paragraph>
<paragraph id="6D27D70FD5574C5EFF72FB3BA647FA18" blockId="13.[151,1437,151,1977]" pageId="13" pageNumber="514">
Tadpoles of 
<taxonomicName id="AA98AC8CD5574C5EFEE9FB3BA649FB14" authorityName="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack" authorityYear="2022" box="[342,559,1231,1257]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="13" pageNumber="514" phylum="Chordata" rank="species" species="celeste">
<emphasis id="5FEC0B1DD5574C5EFEE9FB3BA649FB14" box="[342,559,1231,1257]" italics="true" pageId="13" pageNumber="514">Tlalocohyla celeste</emphasis>
</taxonomicName>
differ most notably from other tadpoles in the genus as follows (Gosner stages in parenthesis): in tadpoles of 
<taxonomicName id="AA98AC8CD5574C5EFE4BFB07A612FAF0" baseAuthorityName="Gunther" baseAuthorityYear="1901" box="[500,628,1267,1293]" class="Amphibia" family="Hylidae" genus="Hyla" kingdom="Animalia" order="Anura" pageId="13" pageNumber="514" phylum="Chordata" rank="species" species="godmani">
<emphasis id="5FEC0B1DD5574C5EFE4BFB07A612FAF0" box="[500,628,1267,1293]" italics="true" pageId="13" pageNumber="514">T. godmani</emphasis>
</taxonomicName>
(38), 
<taxonomicName id="AA98AC8CD5574C5EFD03FB07A743FAF0" baseAuthorityName="Gaige &amp; Stuart" baseAuthorityYear="1934" box="[700,805,1267,1293]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="13" pageNumber="514" phylum="Chordata" rank="species" species="loquax">
<emphasis id="5FEC0B1DD5574C5EFD03FB07A743FAF0" box="[700,805,1267,1293]" italics="true" pageId="13" pageNumber="514">T. loquax</emphasis>
</taxonomicName>
(26), and 
<taxonomicName id="AA98AC8CD5574C5EFC20FB07A792FAF1" baseAuthorityName="Gunther" baseAuthorityYear="1901" box="[927,1012,1267,1292]" class="Amphibia" family="Hylidae" genus="Hyla" kingdom="Animalia" order="Anura" pageId="13" pageNumber="514" phylum="Chordata" rank="species" species="picta">
<emphasis id="5FEC0B1DD5574C5EFC20FB07A792FAF1" box="[927,1012,1267,1292]" italics="true" pageId="13" pageNumber="514">T. picta</emphasis>
</taxonomicName>
(38), the dorsal fin originates on the body at the level of, or anterior to the location of the spiracle (
<bibRefCitation id="0909AAFED5574C5EFCF1FAE3A7DDFACC" author="Lee, J. C." box="[846,955,1303,1329]" pageId="13" pageNumber="514" refId="ref13466" refString="Lee, J. C. (1996) The Amphibians and Reptiles of the Yucatan Peninsula. Cornell University Press, Ithaca, New York, 500 pp." type="book" year="1996">Lee 1996</bibRefCitation>
; 
<bibRefCitation id="0909AAFED5574C5EFC78FAE3A01FFACC" author="Duellman, W. E." box="[967,1145,1303,1329]" pageId="13" pageNumber="514" refId="ref12361" refString="Duellman, W. E. (2001) The Hylid Frogs of Middle America. Society for the Study of Amphibians and Reptiles, Lawrence, Kansas, 1158 pp." type="book" year="2001">Duellman 2001</bibRefCitation>
), whereas in 
<taxonomicName id="AA98AC8CD5574C5EFAB4FAE3A115FACC" authorityName="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack" authorityYear="2022" box="[1291,1395,1303,1329]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="13" pageNumber="514" phylum="Chordata" rank="species" species="celeste">
<emphasis id="5FEC0B1DD5574C5EFAB4FAE3A115FACC" box="[1291,1395,1303,1329]" italics="true" pageId="13" pageNumber="514">T. celeste</emphasis>
</taxonomicName>
the dorsal fin originates at the base of the tail. Tadpoles of 
<taxonomicName id="AA98AC8CD5574C5EFD41FACFA700FAA8" baseAuthorityName="Boulenger" baseAuthorityYear="1902" box="[766,870,1339,1365]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="13" pageNumber="514" phylum="Chordata" rank="species" species="smithii">
<emphasis id="5FEC0B1DD5574C5EFD41FACFA700FAA8" box="[766,870,1339,1365]" italics="true" pageId="13" pageNumber="514">T. smithii</emphasis>
</taxonomicName>
(37) are most similar to those of 
<taxonomicName id="AA98AC8CD5574C5EFB63FACFA125FAA8" authorityName="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack" authorityYear="2022" box="[1244,1347,1339,1365]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="13" pageNumber="514" phylum="Chordata" rank="species" species="celeste">
<emphasis id="5FEC0B1DD5574C5EFB63FACFA125FAA8" box="[1244,1347,1339,1365]" italics="true" pageId="13" pageNumber="514">T. celeste</emphasis>
</taxonomicName>
as their dorsal tail fin starts close to the tail base. However, tadpoles of 
<taxonomicName id="AA98AC8CD5574C5EFCDBFAABA7AAFA84" baseAuthorityName="Boulenger" baseAuthorityYear="1902" box="[868,972,1375,1401]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="13" pageNumber="514" phylum="Chordata" rank="species" species="smithii">
<emphasis id="5FEC0B1DD5574C5EFCDBFAABA7AAFA84" box="[868,972,1375,1401]" italics="true" pageId="13" pageNumber="514">T. smithii</emphasis>
</taxonomicName>
differ in having the posterior third of the tail black and the height of their dorsal fin is relatively constant throughout until it tapers to a fine tip (
<bibRefCitation id="0909AAFED5574C5EFA8AFA77A4B5FA3C" author="Caldwell, J. P." pageId="13" pageNumber="514" pagination="1004 - 1006" refId="ref12192" refString="Caldwell, J. P. (1986) A description of the tadpole of Hyla smithii with comments on tail coloration. Copeia, 1986 (4), 1004 - 1006." type="journal article" year="1986">Caldwell 1986</bibRefCitation>
). Tadpoles of 
<taxonomicName id="AA98AC8CD5574C5EFECDFA53A5BDFA3C" authorityName="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack" authorityYear="2022" box="[370,475,1447,1473]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="13" pageNumber="514" phylum="Chordata" rank="species" species="celeste">
<emphasis id="5FEC0B1DD5574C5EFECDFA53A5BDFA3C" box="[370,475,1447,1473]" italics="true" pageId="13" pageNumber="514">T. celeste</emphasis>
</taxonomicName>
lack a black tail tip and their dorsal fin increases in height towards the middle of the tail before tapering towards the tip.
</paragraph>
<paragraph id="6D27D70FD5574C5EFF78FA1AA07CF8D4" blockId="13.[151,1437,151,1977]" pageId="13" pageNumber="514">
<emphasis id="5FEC0B1DD5574C5EFF78FA1AA5D6F9F4" bold="true" box="[199,432,1518,1545]" pageId="13" pageNumber="514">Advertisement call.</emphasis>
The vocal repertoire of 
<taxonomicName id="AA98AC8CD5574C5EFD7AFA1BA7F9F9F4" authorityName="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack" authorityYear="2022" box="[709,927,1519,1545]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="13" pageNumber="514" phylum="Chordata" rank="species" species="celeste">
<emphasis id="5FEC0B1DD5574C5EFD7AFA1BA7F9F9F4" box="[709,927,1519,1545]" italics="true" pageId="13" pageNumber="514">Tlalocohyla celeste</emphasis>
</taxonomicName>
is composed of at least three call 
<typeStatus id="B22369ADD5574C5EFA9CFA1BA104F9F4" box="[1315,1378,1519,1545]" pageId="13" pageNumber="514">types</typeStatus>
. Call 
<typeStatus id="B22369ADD5574C5EFF28F9E7A4A1F9D0" box="[151,199,1555,1581]" pageId="13" pageNumber="514">type</typeStatus>
I (
<figureCitation id="F5A3CB8AD5574C5EFF58F9E7A506F9D0" box="[231,352,1555,1581]" captionStart="FIGURE 8" captionStartId="14.[152,255,1865,1889]" captionTargetBox="[178,1406,195,1825]" captionTargetId="figure-29@14.[164,1423,181,1841]" captionTargetPageId="14" captionText="FIGURE 8. Calls repertory of Tlalocohyla celeste sp. nov. Call type I: (A) oscillogram of a call series type I, first growing in amplitude and the second and third with similar amplitude notes; (B) oscillogram and (C) spectrogram of a call type I with 13 notes; (D) power spectrum and (E) oscillogram of a single note. Call type II: (F) oscillogram and (G) spectrogram of a call group: call type I following call type II; (H) oscillogram and (I) power spectrum of the single squeak-like note in call Type II. Dotted red line indicates the dominant frequency." figureDoi="http://doi.org/10.5281/zenodo.7037414" httpUri="https://zenodo.org/record/7037414/files/figure.png" pageId="13" pageNumber="514">Fig. 8A–C</figureCitation>
) is composed of a trill of notes, with average call duration of 
<geoCoordinate id="08ACB1C8D5574C5EFBACF9E7A030F9D0" box="[1043,1110,1555,1581]" degrees="0.34" direction="south" orientation="latitude" pageId="13" pageNumber="514" precision="555" value="-0.34">0.34 s</geoCoordinate>
(0.30– 
<geoCoordinate id="08ACB1C8D5574C5EFB1AF9E7A08CF9D0" box="[1189,1258,1555,1581]" degrees="0.39" direction="south" orientation="latitude" pageId="13" pageNumber="514" precision="555" value="-0.39">0.39 s</geoCoordinate>
; n=17), emitted at intervals of 2–10 s (n=14) and average call repetition rate of 12 calls/minute. Call 
<typeStatus id="B22369ADD5574C5EFBDBF9C3A0F2F9AC" box="[1124,1172,1591,1617]" pageId="13" pageNumber="514">type</typeStatus>
I is composed of 9–13 notes, each note having average duration of 
<geoCoordinate id="08ACB1C8D5574C5EFD31F9AFA6B4F988" box="[654,722,1627,1653]" degrees="0.01" direction="south" orientation="latitude" pageId="13" pageNumber="514" precision="555" value="-0.01">0.01 s</geoCoordinate>
(0.01– 
<geoCoordinate id="08ACB1C8D5574C5EFC9EF9AFA70FF988" box="[801,873,1627,1653]" degrees="0.02" direction="south" orientation="latitude" pageId="13" pageNumber="514" precision="555" value="-0.02">0.02 s</geoCoordinate>
; n=17), with a note repetition rate of 33.4 notes/s and inter-note interval of 
<geoCoordinate id="08ACB1C8D5574C5EFE06F98BA598F964" box="[441,510,1663,1689]" degrees="0.01" direction="south" orientation="latitude" pageId="13" pageNumber="514" precision="555" value="-0.01">0.01 s</geoCoordinate>
(0.01– 
<geoCoordinate id="08ACB1C8D5574C5EFDF3F98BA6F2F964" box="[588,660,1663,1689]" degrees="0.02" direction="south" orientation="latitude" pageId="13" pageNumber="514" precision="555" value="-0.02">0.02 s</geoCoordinate>
; n=17). Notes that comprise the call’s trill-pattern can be modulated to increase amplitude towards the end of the call or display similar amplitude throughout (
<figureCitation id="F5A3CB8AD5574C5EFB31F957A08EF940" box="[1166,1256,1699,1725]" captionStart="FIGURE 8" captionStartId="14.[152,255,1865,1889]" captionTargetBox="[178,1406,195,1825]" captionTargetId="figure-29@14.[164,1423,181,1841]" captionTargetPageId="14" captionText="FIGURE 8. Calls repertory of Tlalocohyla celeste sp. nov. Call type I: (A) oscillogram of a call series type I, first growing in amplitude and the second and third with similar amplitude notes; (B) oscillogram and (C) spectrogram of a call type I with 13 notes; (D) power spectrum and (E) oscillogram of a single note. Call type II: (F) oscillogram and (G) spectrogram of a call group: call type I following call type II; (H) oscillogram and (I) power spectrum of the single squeak-like note in call Type II. Dotted red line indicates the dominant frequency." figureDoi="http://doi.org/10.5281/zenodo.7037414" httpUri="https://zenodo.org/record/7037414/files/figure.png" pageId="13" pageNumber="514">Fig. 8A</figureCitation>
). Call dominant frequency ranges 3.80–4.30 kHz (
<figureCitation id="F5A3CB8AD5574C5EFDAEF933A60DF91C" box="[529,619,1735,1761]" captionStart="FIGURE 8" captionStartId="14.[152,255,1865,1889]" captionTargetBox="[178,1406,195,1825]" captionTargetId="figure-29@14.[164,1423,181,1841]" captionTargetPageId="14" captionText="FIGURE 8. Calls repertory of Tlalocohyla celeste sp. nov. Call type I: (A) oscillogram of a call series type I, first growing in amplitude and the second and third with similar amplitude notes; (B) oscillogram and (C) spectrogram of a call type I with 13 notes; (D) power spectrum and (E) oscillogram of a single note. Call type II: (F) oscillogram and (G) spectrogram of a call group: call type I following call type II; (H) oscillogram and (I) power spectrum of the single squeak-like note in call Type II. Dotted red line indicates the dominant frequency." figureDoi="http://doi.org/10.5281/zenodo.7037414" httpUri="https://zenodo.org/record/7037414/files/figure.png" pageId="13" pageNumber="514">Fig. 8D</figureCitation>
). Bandwidth values range from 1.13 (0.90–1.40) to 13.0 (10.3–23.0) kHz (n=17). This call has several sparse harmonics and each pulsed note (30–50 pulses per note) displays an initial high amplitude pulse but subsequently decreases to lower amplitude pulses (
<figureCitation id="F5A3CB8AD5574C5EFC09F8FBA068F8D4" box="[950,1038,1807,1833]" captionStart="FIGURE 8" captionStartId="14.[152,255,1865,1889]" captionTargetBox="[178,1406,195,1825]" captionTargetId="figure-29@14.[164,1423,181,1841]" captionTargetPageId="14" captionText="FIGURE 8. Calls repertory of Tlalocohyla celeste sp. nov. Call type I: (A) oscillogram of a call series type I, first growing in amplitude and the second and third with similar amplitude notes; (B) oscillogram and (C) spectrogram of a call type I with 13 notes; (D) power spectrum and (E) oscillogram of a single note. Call type II: (F) oscillogram and (G) spectrogram of a call group: call type I following call type II; (H) oscillogram and (I) power spectrum of the single squeak-like note in call Type II. Dotted red line indicates the dominant frequency." figureDoi="http://doi.org/10.5281/zenodo.7037414" httpUri="https://zenodo.org/record/7037414/files/figure.png" pageId="13" pageNumber="514">Fig. 8E</figureCitation>
).
</paragraph>
<paragraph id="6D27D70FD5574C5EFF78F8C7A114F844" blockId="13.[151,1437,151,1977]" pageId="13" pageNumber="514">
Call type II (
<figureCitation id="F5A3CB8AD5574C5EFEE4F8C7A5A1F8B0" box="[347,455,1843,1869]" captionStart="FIGURE 8" captionStartId="14.[152,255,1865,1889]" captionTargetBox="[178,1406,195,1825]" captionTargetId="figure-29@14.[164,1423,181,1841]" captionTargetPageId="14" captionText="FIGURE 8. Calls repertory of Tlalocohyla celeste sp. nov. Call type I: (A) oscillogram of a call series type I, first growing in amplitude and the second and third with similar amplitude notes; (B) oscillogram and (C) spectrogram of a call type I with 13 notes; (D) power spectrum and (E) oscillogram of a single note. Call type II: (F) oscillogram and (G) spectrogram of a call group: call type I following call type II; (H) oscillogram and (I) power spectrum of the single squeak-like note in call Type II. Dotted red line indicates the dominant frequency." figureDoi="http://doi.org/10.5281/zenodo.7037414" httpUri="https://zenodo.org/record/7037414/files/figure.png" pageId="13" pageNumber="514">Fig. 8F–I</figureCitation>
) is composed of one to three squeak-like modulated pulsatile notes, each note having an average duration of 
<geoCoordinate id="08ACB1C8D5574C5EFE25F8A3A5BBF88C" box="[410,477,1879,1905]" degrees="0.08" direction="south" orientation="latitude" pageId="13" pageNumber="514" precision="555" value="-0.08">0.08 s</geoCoordinate>
(0.07– 
<geoCoordinate id="08ACB1C8D5574C5EFD94F8A3A617F88C" box="[555,625,1879,1905]" degrees="0.10" direction="south" orientation="latitude" pageId="13" pageNumber="514" precision="555" value="-0.1">0.10 s</geoCoordinate>
; n=4), with a note repetition rate of 3.4 notes/s and inter-note interval of 
<geoCoordinate id="08ACB1C8D5574C5EFF28F88FA4BCF868" box="[151,218,1915,1941]" degrees="0.02" direction="south" orientation="latitude" pageId="13" pageNumber="514" precision="555" value="-0.02">0.02 s</geoCoordinate>
(0.01– 
<geoCoordinate id="08ACB1C8D5574C5EFE97F88FA509F868" box="[296,367,1915,1941]" degrees="0.02" direction="south" orientation="latitude" pageId="13" pageNumber="514" precision="555" value="-0.02">0.02 s</geoCoordinate>
; n=4). The dominant frequency ranges from 4.00–4.40 kHz, bandwidth values range from 0.85 (0.40–1.5) to 12.1 (11.2–14.6) kHz (n =4). Call 
<typeStatus id="B22369ADD5574C5EFD10F86BA6B9F844" box="[687,735,1951,1977]" pageId="13" pageNumber="514">type</typeStatus>
II usually was emitted 0.17– 
<geoCoordinate id="08ACB1C8D5574C5EFB9AF86BA001F844" box="[1061,1127,1951,1977]" degrees="0.21" direction="south" orientation="latitude" pageId="13" pageNumber="514" precision="555" value="-0.21">0.21 s</geoCoordinate>
(n = 4) after call 
<typeStatus id="B22369ADD5574C5EFA94F86BA13DF844" box="[1323,1371,1951,1977]" pageId="13" pageNumber="514">type</typeStatus>
I.
</paragraph>
<caption id="39E78787D5544C5DFF27F8BDA6EAF80C" ID-DOI="http://doi.org/10.5281/zenodo.7037414" ID-Zenodo-Dep="7037414" httpUri="https://zenodo.org/record/7037414/files/figure.png" pageId="14" pageNumber="515" startId="14.[152,255,1865,1889]" targetBox="[178,1406,195,1825]" targetPageId="14">
<paragraph id="6D27D70FD5544C5DFF27F8BDA6EAF80C" blockId="14.[151,1437,1865,2033]" pageId="14" pageNumber="515">
<emphasis id="5FEC0B1DD5544C5DFF27F8BDA57FF89C" bold="true" box="[152,281,1865,1889]" pageId="14" pageNumber="515">FIGURE 8.</emphasis>
Calls repertory of 
<taxonomicName id="AA98AC8CD5544C5DFE61F8BDA6C5F89C" authority="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack, 2022" authorityName="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack" authorityYear="2022" box="[478,675,1865,1889]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="14" pageNumber="515" phylum="Chordata" rank="species" species="celeste" status="sp. nov.">
<emphasis id="5FEC0B1DD5544C5DFE61F8BDA6C5F89C" box="[478,675,1865,1889]" italics="true" pageId="14" pageNumber="515">Tlalocohyla celeste</emphasis>
</taxonomicName>
<emphasis id="5FEC0B1DD5544C5DFD14F8BEA71FF89F" bold="true" box="[683,889,1865,1890]" pageId="14" pageNumber="515">
<taxonomicNameLabel id="44DFB666D5544C5DFD14F8BEA69AF89F" box="[683,764,1866,1890]" pageId="14" pageNumber="515" rank="species">sp. nov.</taxonomicNameLabel>
Call type I
</emphasis>
: (A) oscillogram of a call series type I, first growing in amplitude and the second and third with similar amplitude notes; (B) oscillogram and (C) spectrogram of a call type I with 13 notes; (D) power spectrum and (E) oscillogram of a single note. 
<emphasis id="5FEC0B1DD5544C5DFCFBF865A7AEF857" bold="true" box="[836,968,1937,1962]" pageId="14" pageNumber="515">Call type II:</emphasis>
(F) oscillogram and (G) spectrogram of a call group: call type I following call type II; (H) oscillogram and (I) power spectrum of the single squeak-like note in call Type II. Dotted red line indicates the dominant frequency.
</paragraph>
</caption>
<caption id="39E78787D5554C5CFF27F925A0DEF8AB" ID-DOI="http://doi.org/10.5281/zenodo.7037416" ID-Zenodo-Dep="7037416" httpUri="https://zenodo.org/record/7037416/files/figure.png" pageId="15" pageNumber="516" startId="15.[152,255,1745,1769]" targetBox="[168,1418,193,1709]" targetPageId="15">
<paragraph id="6D27D70FD5554C5CFF27F925A0DEF8AB" blockId="15.[151,1437,1745,1878]" pageId="15" pageNumber="516">
<emphasis id="5FEC0B1DD5554C5CFF27F925A57EF917" bold="true" box="[152,280,1745,1770]" pageId="15" pageNumber="516">FIGURE 9.</emphasis>
Call repertoire of 
<taxonomicName id="AA98AC8CD5554C5CFE6DF926A6F1F917" authority="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack, 2022" authorityName="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack" authorityYear="2022" box="[466,663,1746,1770]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="15" pageNumber="516" phylum="Chordata" rank="species" species="celeste" status="sp. nov.">
<emphasis id="5FEC0B1DD5554C5CFE6DF926A6F1F917" box="[466,663,1746,1770]" italics="true" pageId="15" pageNumber="516">Tlalocohyla celeste</emphasis>
</taxonomicName>
<emphasis id="5FEC0B1DD5554C5CFD22F926A7E7F917" bold="true" box="[669,897,1745,1770]" pageId="15" pageNumber="516">
<taxonomicNameLabel id="44DFB666D5554C5CFD22F926A68BF917" box="[669,749,1746,1770]" pageId="15" pageNumber="516" rank="species">sp. nov.</taxonomicNameLabel>
Call type III:
</emphasis>
(A) oscillogram and (B) spectrogram of a call series: call type I following call type III; (C) oscillogram and (D) power spectrum of notes in call type III. Dotted red line indicates the dominant frequency. (E) Oscillogram of call groups in 
<taxonomicName id="AA98AC8CD5554C5CFD58F8EEA7CDF8CF" authority="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack, 2022" authorityName="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack" authorityYear="2022" box="[743,939,1818,1842]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="15" pageNumber="516" phylum="Chordata" rank="species" species="celeste" status="sp. nov.">
<emphasis id="5FEC0B1DD5554C5CFD58F8EEA7CDF8CF" box="[743,939,1818,1842]" italics="true" pageId="15" pageNumber="516">Tlalocohyla celeste</emphasis>
</taxonomicName>
<emphasis id="5FEC0B1DD5554C5CFC0EF8EEA064F8CF" bold="true" box="[945,1026,1818,1842]" pageId="15" pageNumber="516">
<taxonomicNameLabel id="44DFB666D5554C5CFC0EF8EEA064F8CF" box="[945,1026,1818,1842]" pageId="15" pageNumber="516" rank="species">sp. nov.</taxonomicNameLabel>
</emphasis>
Showing a combination of the three call types (type I in orange, type II in green and type III in red) emitted by different males during the chorus.
</paragraph>
</caption>
<paragraph id="6D27D70FD5554C43FF78F870A126FF04" blockId="15.[151,1437,1924,2023]" lastBlockId="16.[151,1437,150,537]" lastPageId="16" lastPageNumber="517" pageId="15" pageNumber="516">
Call type III (
<figureCitation id="F5A3CB8AD5554C5CFED8F870A582F863" box="[359,484,1924,1950]" captionStart="FIGURE 9" captionStartId="15.[152,255,1745,1769]" captionTargetBox="[168,1418,193,1709]" captionTargetId="figure-18@15.[151,1436,181,1722]" captionTargetPageId="15" captionText="FIGURE 9. Call repertoire of Tlalocohyla celeste sp. nov. Call type III: (A) oscillogram and (B) spectrogram of a call series: call type I following call type III; (C) oscillogram and (D) power spectrum of notes in call type III. Dotted red line indicates the dominant frequency. (E) Oscillogram of call groups in Tlalocohyla celeste sp. nov. Showing a combination of the three call types (type I in orange, type II in green and type III in red) emitted by different males during the chorus." figureDoi="http://doi.org/10.5281/zenodo.7037416" httpUri="https://zenodo.org/record/7037416/files/figure.png" pageId="15" pageNumber="516">Fig. 9A–D</figureCitation>
) is a croak of pulsed notes with an average duration of 
<geoCoordinate id="08ACB1C8D5554C5CFBCEF870A0D1F863" box="[1137,1207,1924,1950]" degrees="0.29" direction="south" orientation="latitude" pageId="15" pageNumber="516" precision="555" value="-0.29">0.29 s</geoCoordinate>
(0.21– 
<geoCoordinate id="08ACB1C8D5554C5CFAB8F870A137F863" box="[1287,1361,1924,1950]" degrees="0.34" direction="south" orientation="latitude" pageId="15" pageNumber="516" precision="555" value="-0.34">0.34 s</geoCoordinate>
; n=7), emitted at intervals of 0.35– 
<geoCoordinate id="08ACB1C8D5554C5CFE62F85CA646F83F" box="[477,544,1960,1987]" degrees="1.61" direction="south" orientation="latitude" pageId="15" pageNumber="516" precision="555" value="-1.61">1.61 s</geoCoordinate>
(n=7), with an average call repetition rate of 23 calls/s (n=7). Call 
<typeStatus id="B22369ADD5554C5CFA94F85CA13DF83F" box="[1323,1371,1960,1986]" pageId="15" pageNumber="516">type</typeStatus>
III is composed of 3–5 pulsed notes of modulated amplitude, with an average call duration of 
<geoCoordinate id="08ACB1C8D5554C5CFBC4F838A0D8F81B" box="[1147,1214,1996,2022]" degrees="0.29" direction="south" orientation="latitude" pageId="15" pageNumber="516" precision="555" value="-0.29">0.29 s</geoCoordinate>
(0.21– 
<geoCoordinate id="08ACB1C8D5554C5CFAB3F838A135F81B" box="[1292,1363,1996,2022]" degrees="0.34" direction="south" orientation="latitude" pageId="15" pageNumber="516" precision="555" value="-0.34">0.34 s</geoCoordinate>
; n=7). Each note has an average duration of 
<geoCoordinate id="08ACB1C8D54A4C43FDFEFF63A6E3FF4C" box="[577,645,151,177]" degrees="0.03" direction="south" orientation="latitude" pageId="16" pageNumber="517" precision="555" value="-0.03">0.03 s</geoCoordinate>
(0.03– 
<geoCoordinate id="08ACB1C8D54A4C43FD6CFF63A77CFF4C" box="[723,794,151,177]" degrees="0.04" direction="south" orientation="latitude" pageId="16" pageNumber="517" precision="555" value="-0.04">0.04 s</geoCoordinate>
; n=7), an inter-note interval of 
<geoCoordinate id="08ACB1C8D54A4C43FBC1FF63A0A4FF4C" box="[1150,1218,151,177]" degrees="0.04" direction="south" orientation="latitude" pageId="16" pageNumber="517" precision="555" value="-0.04">0.04 s</geoCoordinate>
(0.03- 
<geoCoordinate id="08ACB1C8D54A4C43FAB4FF62A135FF4C" box="[1291,1363,150,177]" degrees="0.05" direction="south" orientation="latitude" pageId="16" pageNumber="517" precision="555" value="-0.05">0.05 s</geoCoordinate>
; n=7), and 60–90 pulses per note. The dominant frequency ranges from 3.70–4.10 kHz; bandwidth values range from 1.39 (1.10–1.6) to 11.7 (11.1–13.3) kHz (n=7). Call 
<typeStatus id="B22369ADD54A4C43FD18FF2BA6B1FF04" box="[679,727,223,249]" pageId="16" pageNumber="517">type</typeStatus>
III can be emitted alone, after, or preceding call 
<typeStatus id="B22369ADD54A4C43FB46FF2BA14FFF04" box="[1273,1321,223,249]" pageId="16" pageNumber="517">type</typeStatus>
I.
</paragraph>
<paragraph id="6D27D70FD54A4C43FF78FEF7A489FDE4" blockId="16.[151,1437,150,537]" pageId="16" pageNumber="517">
During periods of breeding chorus activity, males have been observed emitting a) only call 
<typeStatus id="B22369ADD54A4C43FB0DFEF7A084FEE0" box="[1202,1250,259,285]" pageId="16" pageNumber="517">type</typeStatus>
I, b) one to 
<specimenCount id="7B9E1C86D54A4C43FADBFEF7A4A1FEBC" pageId="16" pageNumber="517" type="generic" typeStatus="type">three type</specimenCount>
II calls, followed by a single 
<typeStatus id="B22369ADD54A4C43FDB4FED3A65DFEBC" box="[523,571,295,321]" pageId="16" pageNumber="517">type</typeStatus>
I call, c) emit several calls of 
<typeStatus id="B22369ADD54A4C43FC3CFED3A7D5FEBC" box="[899,947,295,321]" pageId="16" pageNumber="517">type</typeStatus>
III followed or preceded by call 
<typeStatus id="B22369ADD54A4C43FAA3FED3A12AFEBC" box="[1308,1356,295,321]" pageId="16" pageNumber="517">type</typeStatus>
I, or d) produce all three call 
<typeStatus id="B22369ADD54A4C43FE38FEBFA5A4FE98" box="[391,450,331,357]" pageId="16" pageNumber="517">types</typeStatus>
sequentially (
<figureCitation id="F5A3CB8AD54A4C43FDE1FEBFA6D2FE98" box="[606,692,331,357]" captionStart="FIGURE 9" captionStartId="15.[152,255,1745,1769]" captionTargetBox="[168,1418,193,1709]" captionTargetId="figure-18@15.[151,1436,181,1722]" captionTargetPageId="15" captionText="FIGURE 9. Call repertoire of Tlalocohyla celeste sp. nov. Call type III: (A) oscillogram and (B) spectrogram of a call series: call type I following call type III; (C) oscillogram and (D) power spectrum of notes in call type III. Dotted red line indicates the dominant frequency. (E) Oscillogram of call groups in Tlalocohyla celeste sp. nov. Showing a combination of the three call types (type I in orange, type II in green and type III in red) emitted by different males during the chorus." figureDoi="http://doi.org/10.5281/zenodo.7037416" httpUri="https://zenodo.org/record/7037416/files/figure.png" pageId="16" pageNumber="517">Fig. 9E</figureCitation>
). Call 
<typeStatus id="B22369ADD54A4C43FD43FEBFA74AFE98" box="[764,812,331,357]" pageId="16" pageNumber="517">type</typeStatus>
I of 
<taxonomicName id="AA98AC8CD54A4C43FCE0FEBFA7A0FE98" authorityName="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack" authorityYear="2022" box="[863,966,331,357]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="16" pageNumber="517" phylum="Chordata" rank="species" species="celeste">
<emphasis id="5FEC0B1DD54A4C43FCE0FEBFA7A0FE98" box="[863,966,331,357]" italics="true" pageId="16" pageNumber="517">T. celeste</emphasis>
</taxonomicName>
is more similar to the trills produced by 
<taxonomicName id="AA98AC8CD54A4C43FA37FEBFA4A9FE75" baseAuthorityName="Gunther" baseAuthorityYear="1901" class="Amphibia" family="Hylidae" genus="Hyla" kingdom="Animalia" order="Anura" pageId="16" pageNumber="517" phylum="Chordata" rank="species" species="picta">
<emphasis id="5FEC0B1DD54A4C43FA37FEBFA4A9FE75" italics="true" pageId="16" pageNumber="517">T. picta</emphasis>
</taxonomicName>
and 
<taxonomicName id="AA98AC8CD54A4C43FEBCFE9BA50FFE74" baseAuthorityName="Boulenger" baseAuthorityYear="1902" box="[259,361,367,393]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="16" pageNumber="517" phylum="Chordata" rank="species" species="smithii">
<emphasis id="5FEC0B1DD54A4C43FEBCFE9BA50FFE74" box="[259,361,367,393]" italics="true" pageId="16" pageNumber="517">T. smithii</emphasis>
</taxonomicName>
but can be differentiated by the following characteristics (condition for 
<taxonomicName id="AA98AC8CD54A4C43FB3EFE9BA081FE74" authorityName="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack" authorityYear="2022" box="[1153,1255,367,393]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="16" pageNumber="517" phylum="Chordata" rank="species" species="celeste">
<emphasis id="5FEC0B1DD54A4C43FB3EFE9BA081FE74" box="[1153,1255,367,393]" italics="true" pageId="16" pageNumber="517">T. celeste</emphasis>
</taxonomicName>
in parentheses): Call 
<typeStatus id="B22369ADD54A4C43FF72FE67A49BFE50" box="[205,253,403,429]" pageId="16" pageNumber="517">type</typeStatus>
I differs primarily in the number of notes per call: the trill speed is faster in 
<taxonomicName id="AA98AC8CD54A4C43FBE1FE67A0D4FE51" baseAuthorityName="Gunther" baseAuthorityYear="1901" box="[1118,1202,403,428]" class="Amphibia" family="Hylidae" genus="Hyla" kingdom="Animalia" order="Anura" pageId="16" pageNumber="517" phylum="Chordata" rank="species" species="picta">
<emphasis id="5FEC0B1DD54A4C43FBE1FE67A0D4FE51" box="[1118,1202,403,428]" italics="true" pageId="16" pageNumber="517">T. picta</emphasis>
</taxonomicName>
= 60–70 notes/s and 
<taxonomicName id="AA98AC8CD54A4C43FF28FE43A499FE2C" baseAuthorityName="Boulenger" baseAuthorityYear="1902" box="[151,255,439,465]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="16" pageNumber="517" phylum="Chordata" rank="species" species="smithii">
<emphasis id="5FEC0B1DD54A4C43FF28FE43A499FE2C" box="[151,255,439,465]" italics="true" pageId="16" pageNumber="517">T. smithii</emphasis>
</taxonomicName>
= 43–51 notes/s than in 
<taxonomicName id="AA98AC8CD54A4C43FDADFE43A61CFE2C" authorityName="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack" authorityYear="2022" box="[530,634,439,465]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="16" pageNumber="517" phylum="Chordata" rank="species" species="celeste">
<emphasis id="5FEC0B1DD54A4C43FDADFE43A61CFE2C" box="[530,634,439,465]" italics="true" pageId="16" pageNumber="517">T. celeste</emphasis>
</taxonomicName>
(25–37 notes/s; n=17). In addition, 
<taxonomicName id="AA98AC8CD54A4C43FBB2FE43A006FE2D" baseAuthorityName="Gunther" baseAuthorityYear="1901" box="[1037,1120,439,464]" class="Amphibia" family="Hylidae" genus="Hyla" kingdom="Animalia" order="Anura" pageId="16" pageNumber="517" phylum="Chordata" rank="species" species="picta">
<emphasis id="5FEC0B1DD54A4C43FBB2FE43A006FE2D" box="[1037,1120,439,464]" italics="true" pageId="16" pageNumber="517">T. picta</emphasis>
</taxonomicName>
and 
<taxonomicName id="AA98AC8CD54A4C43FB28FE43A098FE2C" baseAuthorityName="Boulenger" baseAuthorityYear="1902" box="[1175,1278,439,465]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="16" pageNumber="517" phylum="Chordata" rank="species" species="smithii">
<emphasis id="5FEC0B1DD54A4C43FB28FE43A098FE2C" box="[1175,1278,439,465]" italics="true" pageId="16" pageNumber="517">T. smithii</emphasis>
</taxonomicName>
have a higher dominant frequency, 4.6–6.30 kHz and 4.29–6.64 kHz respectively, when compared with 
<taxonomicName id="AA98AC8CD54A4C43FBC5FE2FA087FE08" authorityName="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack" authorityYear="2022" box="[1146,1249,475,501]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="16" pageNumber="517" phylum="Chordata" rank="species" species="celeste">
<emphasis id="5FEC0B1DD54A4C43FBC5FE2FA087FE08" box="[1146,1249,475,501]" italics="true" pageId="16" pageNumber="517">T. celeste</emphasis>
</taxonomicName>
(3.80–4.30 kHz, n = 28).
</paragraph>
<caption id="39E78787D54A4C43FF27F906A009F8D3" ID-DOI="http://doi.org/10.5281/zenodo.7037418" ID-Zenodo-Dep="7037418" httpUri="https://zenodo.org/record/7037418/files/figure.png" pageId="16" pageNumber="517" startId="16.[152,255,1778,1802]" targetBox="[153,1436,562,1754]" targetPageId="16">
<paragraph id="6D27D70FD54A4C43FF27F906A009F8D3" blockId="16.[151,1436,1778,1838]" pageId="16" pageNumber="517">
<emphasis id="5FEC0B1DD54A4C43FF27F906A540F8F7" bold="true" box="[152,294,1778,1802]" pageId="16" pageNumber="517">FIGURE 10.</emphasis>
Oviposition process of 
<taxonomicName id="AA98AC8CD54A4C43FDA3F906A687F8F7" authority="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack, 2022" authorityName="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack" authorityYear="2022" box="[540,737,1778,1802]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="16" pageNumber="517" phylum="Chordata" rank="species" species="celeste" status="sp. nov.">
<emphasis id="5FEC0B1DD54A4C43FDA3F906A687F8F7" box="[540,737,1778,1802]" italics="true" pageId="16" pageNumber="517">Tlalocohyla celeste</emphasis>
</taxonomicName>
<emphasis id="5FEC0B1DD54A4C43FD57F906A75FF8F7" bold="true" box="[744,825,1778,1802]" pageId="16" pageNumber="517">
<taxonomicNameLabel id="44DFB666D54A4C43FD57F906A75FF8F7" box="[744,825,1778,1802]" pageId="16" pageNumber="517" rank="species">sp. nov.</taxonomicNameLabel>
</emphasis>
(A) A pair in axillary amplexus with egg deposition. (B-D) Oviposition at the end of different leaves. (E) 5 days of development. (F) 9 days of development.
</paragraph>
</caption>
<paragraph id="6D27D70FD54A4C42FF78F8A8A7CAFE98" blockId="16.[151,1436,1884,2019]" lastBlockId="17.[151,1436,151,607]" lastPageId="17" lastPageNumber="518" pageId="16" pageNumber="517">
<emphasis id="5FEC0B1DD54A4C43FF78F8A8A50DF88B" bold="true" box="[199,363,1884,1910]" pageId="16" pageNumber="517">Reproduction</emphasis>
. Males of 
<taxonomicName id="AA98AC8CD54A4C43FE57F8A8A6A4F88B" authorityName="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack" authorityYear="2022" box="[488,706,1884,1910]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="16" pageNumber="517" phylum="Chordata" rank="species" species="celeste">
<emphasis id="5FEC0B1DD54A4C43FE57F8A8A6A4F88B" box="[488,706,1884,1910]" italics="true" pageId="16" pageNumber="517">Tlalocohyla celeste</emphasis>
</taxonomicName>
have been observed calling year-round but display a significant increase in calling activity during periods of heightened precipitation. Vocalizations are generally first observed around 16:00 h and continue throughout the night, until sunrise. Individuals can be heard during the day, especially during periods of heavy rainfall. Most calling activity takes place along the vegetated wetland perimeter, density of calling males decreases towards the center of the wetland where water levels are deeper. Males have been observed calling in a head-down, horizontal, or head-up position, as they perch on vegetation. Individuals occupied a variety of herbaceous plant perches, but most were found on the locally common grass 
<taxonomicName id="AA98AC8CD54B4C42FBC6FF2BA54BFEE1" class="Liliopsida" family="Cyperaceae" genus="Rhynchospora" kingdom="Plantae" order="Poales" pageId="17" pageNumber="518" phylum="Tracheophyta" rank="species" species="corymbosa">
<emphasis id="5FEC0B1DD54B4C42FBC6FF2BA1FAFF04" box="[1145,1436,223,249]" italics="true" pageId="17" pageNumber="518">Rhynchospora corymbosa</emphasis>
(Cyperaceae)
</taxonomicName>
(
<figureCitation id="F5A3CB8AD54B4C42FE84FEF6A519FEE1" box="[315,383,258,284]" captionStart="FIGURE 7" captionStartId="11.[152,255,1910,1934]" captionTargetBox="[154,1435,456,1884]" captionTargetId="figure-148@11.[151,1435,453,1887]" captionTargetPageId="11" captionText="FIGURE 7. Vocal sac of uncollected males of Tlalocohyla celeste sp. nov. (A) Ventral view and vocal sac deflated. (B–D). Calling males perched on vegetation in different positions, note the vocal sac extension." figureDoi="http://doi.org/10.5281/zenodo.7037412" httpUri="https://zenodo.org/record/7037412/files/figure.png" pageId="17" pageNumber="518">Fig. 7</figureCitation>
). Calling males are positioned 
<quantity id="AA607AEAD54B4C42FD66FEF6A752FEE0" box="[729,820,258,285]" metricMagnitude="0" metricUnit="m" metricValue="1.55" metricValueMax="3.0" metricValueMin="0.1" pageId="17" pageNumber="518" unit="m" value="1.55" valueMax="3.0" valueMin="0.1">0.1–3 m</quantity>
above the ground, either calling in isolation or in small groups, with individuals often perched as little as 
<quantity id="AA607AEAD54B4C42FD67FED2A72CFEBC" box="[728,842,294,321]" metricMagnitude="-1" metricUnit="m" metricValue="1.75" metricValueMax="2.0" metricValueMin="1.5" pageId="17" pageNumber="518" unit="cm" value="17.5" valueMax="20.0" valueMin="15.0">15–20 cm</quantity>
from one another. Satellite male behavior has been observed in this species, a trait also reported for 
<taxonomicName id="AA98AC8CD54B4C42FD09FEBFA7CEFE98" authority="(Roble 1985)" baseAuthorityName="Roble" baseAuthorityYear="1985" box="[694,936,330,357]" class="Amphibia" family="Hylidae" genus="Hyla" kingdom="Animalia" order="Anura" pageId="17" pageNumber="518" phylum="Chordata" rank="species" species="picta">
<emphasis id="5FEC0B1DD54B4C42FD09FEBFA76FFE99" box="[694,777,331,356]" italics="true" pageId="17" pageNumber="518">T. picta</emphasis>
(
<bibRefCitation id="0909AAFED54B4C42FCA7FEBEA7C6FE98" author="Roble, S. M." box="[792,928,330,357]" pageId="17" pageNumber="518" pagination="432 - 436" refId="ref13998" refString="Roble, S. M. (1985) Observations on Satellite Males in Hyla chrysoscelis, Hyla picta, and Pseudacris triseriata. Journal of Herpetology, 19 (3), 432 - 436." type="journal article" year="1985">Roble 1985</bibRefCitation>
)
</taxonomicName>
.
</paragraph>
<paragraph id="6D27D70FD54B4C42FF78FE99A705FE0E" blockId="17.[151,1436,151,607]" pageId="17" pageNumber="518">
Females of this species, detected less frequently than males, were observed active in the periphery of the wetland and moved around calling males. On several occasions, females were found in the interior of the forest that surrounds the wetland, some 
<quantity id="AA607AEAD54B4C42FE5CFE41A678FE2D" box="[483,542,437,464]" metricMagnitude="1" metricUnit="m" metricValue="2.0" pageId="17" pageNumber="518" unit="m" value="20.0">20 m</quantity>
from the water’s edge. Gravid females were first observed on 
<date id="1926F1CFD54B4C42FB57FE42A1FAFE32" box="[1256,1436,437,464]" pageId="17" pageNumber="518" value="2021-06-12">June 12th, 2021</date>
(
<figureCitation id="F5A3CB8AD54B4C42FF20FE2DA490FE0E" box="[159,246,473,499]" captionStart="FIGURE 4" captionStartId="8.[152,255,1910,1934]" captionTargetBox="[172,1415,182,1885]" captionTargetId="figure-29@8.[172,1415,181,1886]" captionTargetPageId="8" captionText="FIGURE 4. Variation in dorsal pattern of Tlalocohyla celeste sp. nov. (A–B) male holotype of Tlalocohyla celeste sp. nov. (UCR 23700) in life. (C) Size differences between females and males. (D) Typical nocturnal pattern of males. (E) Female with the oocytes visible by transparency in the body. (F) Diurnal pattern of adults, males and females, note the white iris chance. (G) Juvenile. (H) Diurnal pattern with red-brown iris." figureDoi="http://doi.org/10.5281/zenodo.7037399" httpUri="https://zenodo.org/record/7037399/files/figure.png" pageId="17" pageNumber="518">Fig. 4E</figureCitation>
), followed by subsequent sightings through September.
</paragraph>
<paragraph id="6D27D70FD54B4C42FF78FE08A0EAFDA3" blockId="17.[151,1436,151,607]" pageId="17" pageNumber="518">
<taxonomicName id="AA98AC8CD54B4C42FF78FE08A5F8FDEB" authorityName="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack" authorityYear="2022" box="[199,414,508,534]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="17" pageNumber="518" phylum="Chordata" rank="species" species="celeste">
<emphasis id="5FEC0B1DD54B4C42FF78FE08A5F8FDEB" box="[199,414,508,534]" italics="true" pageId="17" pageNumber="518">Tlalocohyla celeste</emphasis>
</taxonomicName>
exhibits axillary amplexus (
<bibRefCitation id="0909AAFED54B4C42FD6DFE08A786FDEB" author="Duellman, W. E. &amp; Trueb, L." box="[722,992,508,535]" pageId="17" pageNumber="518" refId="ref12393" refString="Duellman, W. E. &amp; Trueb, L. (1986) Biology of Amphibians. The Johns Hopkins University Press, Baltimore, Maryland and Ithaca, New York, 670 pp." type="book" year="1986">Duellman &amp; Trueb 1986</bibRefCitation>
) (
<figureCitation id="F5A3CB8AD54B4C42FC49FE08A02FFDEA" box="[1014,1097,508,535]" captionStart="FIGURE 4" captionStartId="8.[152,255,1910,1934]" captionTargetBox="[172,1415,182,1885]" captionTargetId="figure-29@8.[172,1415,181,1886]" captionTargetPageId="8" captionText="FIGURE 4. Variation in dorsal pattern of Tlalocohyla celeste sp. nov. (A–B) male holotype of Tlalocohyla celeste sp. nov. (UCR 23700) in life. (C) Size differences between females and males. (D) Typical nocturnal pattern of males. (E) Female with the oocytes visible by transparency in the body. (F) Diurnal pattern of adults, males and females, note the white iris chance. (G) Juvenile. (H) Diurnal pattern with red-brown iris." figureDoi="http://doi.org/10.5281/zenodo.7037399" httpUri="https://zenodo.org/record/7037399/files/figure.png" pageId="17" pageNumber="518">Fig. 4C</figureCitation>
and 
<figureCitation id="F5A3CB8AD54B4C42FBC5FE09A0C9FDEA" box="[1146,1199,509,535]" captionStart="FIGURE 10" captionStartId="16.[152,255,1778,1802]" captionTargetBox="[153,1436,562,1754]" captionTargetId="figure-237@16.[152,1436,561,1754]" captionTargetPageId="16" captionText="FIGURE 10. Oviposition process of Tlalocohyla celeste sp. nov. (A) A pair in axillary amplexus with egg deposition. (B-D) Oviposition at the end of different leaves. (E) 5 days of development. (F) 9 days of development." figureDoi="http://doi.org/10.5281/zenodo.7037418" httpUri="https://zenodo.org/record/7037418/files/figure.png" pageId="17" pageNumber="518">10A</figureCitation>
). The first amplectant pair was discovered on 
<date id="1926F1CFD54B4C42FE23FDD5A62AFDC7" box="[412,588,544,571]" pageId="17" pageNumber="518" value="2021-06-13">June 13th, 2021</date>
at 5:00 h. Amplexus and oviposition have been observed at night and during the pre-dawn morning hours. The amplecting pair separates immediately after oviposition.
</paragraph>
<caption id="39E78787D54B4C42FF27F840A6B9F80D" ID-DOI="http://doi.org/10.5281/zenodo.7037420" ID-Zenodo-Dep="7037420" httpUri="https://zenodo.org/record/7037420/files/figure.png" pageId="17" pageNumber="518" startId="17.[152,255,1972,1996]" targetBox="[159,1429,630,1948]" targetPageId="17">
<paragraph id="6D27D70FD54B4C42FF27F840A6B9F80D" blockId="17.[151,1437,1972,2032]" pageId="17" pageNumber="518">
<emphasis id="5FEC0B1DD54B4C42FF27F840A543F831" bold="true" box="[152,293,1972,1996]" pageId="17" pageNumber="518">FIGURE 11.</emphasis>
Metamorphosis of 
<taxonomicName id="AA98AC8CD54B4C42FE52F840A6D4F831" authority="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack, 2022" authorityName="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack" authorityYear="2022" box="[493,690,1972,1996]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="17" pageNumber="518" phylum="Chordata" rank="species" species="celeste" status="sp. nov.">
<emphasis id="5FEC0B1DD54B4C42FE52F840A6D4F831" box="[493,690,1972,1996]" italics="true" pageId="17" pageNumber="518">Tlalocohyla celeste</emphasis>
</taxonomicName>
<emphasis id="5FEC0B1DD54B4C42FD06F840A76CF831" bold="true" box="[697,778,1972,1996]" pageId="17" pageNumber="518">
<taxonomicNameLabel id="44DFB666D54B4C42FD06F840A76CF831" box="[697,778,1972,1996]" pageId="17" pageNumber="518" rank="species">sp. nov.</taxonomicNameLabel>
</emphasis>
(A) Tadpole at stage 35. (B) Metamorph of 11 mm of SVL. (C) Recently metamorphosed juvenile of 7 mm. (D) Juvenile.
</paragraph>
</caption>
<caption id="39E78787D5484C41FF27F863A66AF829" ID-DOI="http://doi.org/10.5281/zenodo.7037423" ID-Zenodo-Dep="7037423" httpUri="https://zenodo.org/record/7037423/files/figure.png" pageId="18" pageNumber="519" startId="18.[152,255,1943,1967]" targetBox="[205,1379,199,1905]" targetPageId="18">
<paragraph id="6D27D70FD5484C41FF27F863A66AF829" blockId="18.[151,1436,1943,2004]" pageId="18" pageNumber="519">
<emphasis id="5FEC0B1DD5484C41FF27F863A541F852" bold="true" box="[152,295,1943,1967]" pageId="18" pageNumber="519">FIGURE 12.</emphasis>
<taxonomicName id="AA98AC8CD5484C41FE91F86CA592F84D" authority="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack, 2022" authorityName="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack" authorityYear="2022" box="[302,500,1944,1968]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="18" pageNumber="519" phylum="Chordata" rank="species" species="celeste" status="sp. nov.">
<emphasis id="5FEC0B1DD5484C41FE91F86CA592F84D" box="[302,500,1944,1968]" italics="true" pageId="18" pageNumber="519">Tlalocohyla celeste</emphasis>
</taxonomicName>
<emphasis id="5FEC0B1DD5484C41FE43F86CA628F84D" bold="true" box="[508,590,1944,1968]" pageId="18" pageNumber="519">
<taxonomicNameLabel id="44DFB666D5484C41FE43F86CA628F84D" box="[508,590,1944,1968]" pageId="18" pageNumber="519" rank="species">sp. nov.</taxonomicNameLabel>
</emphasis>
tadpole at stage 34 (35) according to 
<bibRefCitation id="0909AAFED5484C41FC66F86CA017F84D" author="Gosner, K. L." box="[985,1137,1944,1968]" pageId="18" pageNumber="519" pagination="183 - 190" refId="ref12911" refString="Gosner, K. L. (1960) A simplified table for staging anuran embryos and larvae with notes on identification. Herpetologica, 16 (3), 183 - 190." type="journal article" year="1960">Gosner (1960)</bibRefCitation>
. (A) Lateral view, (B) dorsal view, (C) ventral view, (D) oral disc.
</paragraph>
</caption>
<paragraph id="6D27D70FD5494C40FF78FF63A6C7FE50" blockId="19.[151,1437,151,861]" pageId="19" pageNumber="520">
All egg masses observed to date have been deposited at the tip, or along the drooping edge of leaves located directly above the water (
<figureCitation id="F5A3CB8AD5494C40FE00FF4FA673FF28" box="[447,533,187,213]" captionStart="FIGURE 10" captionStartId="16.[152,255,1778,1802]" captionTargetBox="[153,1436,562,1754]" captionTargetId="figure-237@16.[152,1436,561,1754]" captionTargetPageId="16" captionText="FIGURE 10. Oviposition process of Tlalocohyla celeste sp. nov. (A) A pair in axillary amplexus with egg deposition. (B-D) Oviposition at the end of different leaves. (E) 5 days of development. (F) 9 days of development." figureDoi="http://doi.org/10.5281/zenodo.7037418" httpUri="https://zenodo.org/record/7037418/files/figure.png" pageId="19" pageNumber="520">Fig. 10</figureCitation>
). This indicates that 
<taxonomicName id="AA98AC8CD5494C40FCB7FF4FA714FF28" authorityName="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack" authorityYear="2022" box="[776,882,187,213]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="19" pageNumber="520" phylum="Chordata" rank="species" species="celeste">
<emphasis id="5FEC0B1DD5494C40FCB7FF4FA714FF28" box="[776,882,187,213]" italics="true" pageId="19" pageNumber="520">T. celeste</emphasis>
</taxonomicName>
employs reproductive mode 24 (
<emphasis id="5FEC0B1DD5494C40FB4DFF4FA17AFF28" box="[1266,1308,187,213]" italics="true" pageId="19" pageNumber="520">fide</emphasis>
<bibRefCitation id="0909AAFED5494C40FA99FF4FA57AFF04" author="Haddad, C. F. B. &amp; Prado, C. P. A." pageId="19" pageNumber="520" pagination="207 - 217" refId="ref13072" refString="Haddad, C. F. B. &amp; Prado, C. P. A. (2005) Reproductive modes in frogs and their unexpected diversity in the Atlantic Forest of Brazil. BioScience, 55 (3), 207 - 217. https: // doi. org / 10.1641 / 0006 - 3568 (2005) 055 [0207: RMIFAT] 2.0. CO; 2" type="journal article" year="2005">Haddad &amp; Prado 2005</bibRefCitation>
) where arboreal eggs hatch into exotrophic tadpoles that drop into lentic water. Egg masses contain 20–61 unpigmented eggs (n=8) (
<figureCitation id="F5A3CB8AD5494C40FDB2FEF7A6FEFEE0" box="[525,664,259,285]" captionStart="FIGURE 10" captionStartId="16.[152,255,1778,1802]" captionTargetBox="[153,1436,562,1754]" captionTargetId="figure-237@16.[152,1436,561,1754]" captionTargetPageId="16" captionText="FIGURE 10. Oviposition process of Tlalocohyla celeste sp. nov. (A) A pair in axillary amplexus with egg deposition. (B-D) Oviposition at the end of different leaves. (E) 5 days of development. (F) 9 days of development." figureDoi="http://doi.org/10.5281/zenodo.7037418" httpUri="https://zenodo.org/record/7037418/files/figure.png" pageId="19" pageNumber="520">Fig. 10B–D</figureCitation>
). Measurements taken from three different egg masses indicate that each clutch occupies an area between 
<quantity id="AA607AEAD5494C40FDF3FED3A6A1FEBC" box="[588,711,295,321]" metricMagnitude="-2" metricUnit="m" metricValue="1.7" metricValueMax="2.2" metricValueMin="1.2" pageId="19" pageNumber="520" unit="mm" value="17.0" valueMax="22.0" valueMin="12.0">12–22 mm</quantity>
in width and 
<quantity id="AA607AEAD5494C40FCD8FED3A784FEBC" box="[871,994,295,321]" metricMagnitude="-2" metricUnit="m" metricValue="2.55" metricValueMax="2.8" metricValueMin="2.3" pageId="19" pageNumber="520" unit="mm" value="25.5" valueMax="28.0" valueMin="23.0">23–28 mm</quantity>
in length; eggs measure an average of 2.0 mm in diameter (without the clear jelly capsules). One gravid female maintained in captivity for two days deposited three clutches containing 23, 45, and 
<specimenCount id="7B9E1C86D5494C40FD15FE9BA766FE74" box="[682,768,367,393]" pageId="19" pageNumber="520" type="egg">61 eggs</specimenCount>
respectively, indicating that females can engage in multiple oviposition events during each breeding cycle.
</paragraph>
<paragraph id="6D27D70FD5494C40FF78FE43A025FDC0" blockId="19.[151,1437,151,861]" pageId="19" pageNumber="520">
Early embryos, observed five days after oviposition, were uniformly white with visible external gills (
<figureCitation id="F5A3CB8AD5494C40FA93FE43A1F6FE2C" box="[1324,1424,439,465]" captionStart="FIGURE 10" captionStartId="16.[152,255,1778,1802]" captionTargetBox="[153,1436,562,1754]" captionTargetId="figure-237@16.[152,1436,561,1754]" captionTargetPageId="16" captionText="FIGURE 10. Oviposition process of Tlalocohyla celeste sp. nov. (A) A pair in axillary amplexus with egg deposition. (B-D) Oviposition at the end of different leaves. (E) 5 days of development. (F) 9 days of development." figureDoi="http://doi.org/10.5281/zenodo.7037418" httpUri="https://zenodo.org/record/7037418/files/figure.png" pageId="19" pageNumber="520">Fig. 10E</figureCitation>
). Seven days after oviposition, these embryos were light brown and marked with small black spots, particularly on the tail; the ventral region remained white. Nine days after oviposition, the embryos were brown with additional spots present on body and tail (
<figureCitation id="F5A3CB8AD5494C40FE0BFDD7A671FDC0" box="[436,535,547,573]" captionStart="FIGURE 10" captionStartId="16.[152,255,1778,1802]" captionTargetBox="[153,1436,562,1754]" captionTargetId="figure-237@16.[152,1436,561,1754]" captionTargetPageId="16" captionText="FIGURE 10. Oviposition process of Tlalocohyla celeste sp. nov. (A) A pair in axillary amplexus with egg deposition. (B-D) Oviposition at the end of different leaves. (E) 5 days of development. (F) 9 days of development." figureDoi="http://doi.org/10.5281/zenodo.7037418" httpUri="https://zenodo.org/record/7037418/files/figure.png" pageId="19" pageNumber="520">Fig. 10F</figureCitation>
). The tadpoles hatched ten days after oviposition.
</paragraph>
<paragraph id="6D27D70FD5494C40FF78FDB3A091FD30" blockId="19.[151,1437,151,861]" pageId="19" pageNumber="520">
Egg masses were observed from June to November. Metamorphs and small juveniles were observed between November and June. Based on observations derived from tadpoles reared in ex-situ conditions, larval development appears to be slow. Three months after hatching, tadpoles reared under controlled conditions remained at Gosner stages 36–39 (
<bibRefCitation id="0909AAFED5494C40FE88FD47A5ABFD30" author="Gosner, K. L." box="[311,461,691,717]" pageId="19" pageNumber="520" pagination="183 - 190" refId="ref12911" refString="Gosner, K. L. (1960) A simplified table for staging anuran embryos and larvae with notes on identification. Herpetologica, 16 (3), 183 - 190." type="journal article" year="1960">Gosner 1960</bibRefCitation>
). A recent metamorph was measured to have a SVL of 
<quantity id="AA607AEAD5494C40FB8BFD47A013FD30" box="[1076,1141,691,717]" metricMagnitude="-3" metricUnit="m" metricValue="7.0" pageId="19" pageNumber="520" unit="mm" value="7.0">7 mm</quantity>
(
<figureCitation id="F5A3CB8AD5494C40FB3BFD47A08FFD30" box="[1156,1257,691,717]" captionStart="FIGURE 11" captionStartId="17.[152,255,1972,1996]" captionTargetBox="[159,1429,630,1948]" captionTargetId="figure-229@17.[159,1429,630,1948]" captionTargetPageId="17" captionText="FIGURE 11. Metamorphosis of Tlalocohyla celeste sp. nov. (A) Tadpole at stage 35. (B) Metamorph of 11 mm of SVL. (C) Recently metamorphosed juvenile of 7 mm. (D) Juvenile." figureDoi="http://doi.org/10.5281/zenodo.7037420" httpUri="https://zenodo.org/record/7037420/files/figure.png" pageId="19" pageNumber="520">Fig. 11C</figureCitation>
).
</paragraph>
<paragraph id="6D27D70FD5494C40FF78FD23A159FCA0" blockId="19.[151,1437,151,861]" pageId="19" pageNumber="520">
The following anurans were observed to occur syntopically and likely share resources with 
<taxonomicName id="AA98AC8CD5494C40FB11FD23A175FD0C" authorityName="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack" authorityYear="2022" box="[1198,1299,727,753]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="19" pageNumber="520" phylum="Chordata" rank="species" species="celeste">
<emphasis id="5FEC0B1DD5494C40FB11FD23A175FD0C" box="[1198,1299,727,753]" italics="true" pageId="19" pageNumber="520">T. celeste</emphasis>
</taxonomicName>
: 
<emphasis id="5FEC0B1DD5494C40FA9FFD23A6BAFCA0" italics="true" pageId="19" pageNumber="520">
<taxonomicName id="AA98AC8CD5494C40FA9FFD23A56CFCE8" baseAuthorityName="Cope" baseAuthorityYear="1862" class="Amphibia" family="Phyllomedusidae" genus="Agalychnis" kingdom="Animalia" order="Anura" pageId="19" pageNumber="520" phylum="Chordata" rank="species" species="callidryas">Agalychnis callidryas</taxonomicName>
, 
<taxonomicName id="AA98AC8CD5494C40FEAAFD0FA5DCFCE8" baseAuthorityName="Fouquette" baseAuthorityYear="1961" box="[277,442,763,789]" class="Amphibia" family="Hylidae" genus="Boana" kingdom="Animalia" order="Anura" pageId="19" pageNumber="520" phylum="Chordata" rank="species" species="rufitela">Boana rufitela</taxonomicName>
, 
<taxonomicName id="AA98AC8CD5494C40FE7AFD0FA69CFCE8" baseAuthorityName="Cope" baseAuthorityYear="1874" box="[453,762,763,789]" class="Amphibia" family="Hylidae" genus="Dendropsophus" kingdom="Animalia" order="Anura" pageId="19" pageNumber="520" phylum="Chordata" rank="species" species="ebraccatus">Dendropsophus ebraccatus</taxonomicName>
, 
<taxonomicName id="AA98AC8CD5494C40FCBBFD0FA004FCE8" baseAuthorityName="Cope" baseAuthorityYear="1886" box="[772,1122,763,789]" class="Amphibia" family="Hylidae" genus="Dendropsophus" kingdom="Animalia" order="Anura" pageId="19" pageNumber="520" phylum="Chordata" rank="species" species="microcephalus">Dendropsophus microcephalus</taxonomicName>
, 
<taxonomicName id="AA98AC8CD5494C40FBD2FD0FA48DFCC4" authority=", Incilius" authorityName="Incilius" class="Amphibia" family="Hylidae" genus="Dendropsophus" kingdom="Animalia" order="Anura" pageId="19" pageNumber="520" phylum="Chordata" rank="species" species="phlebodes">Dendropsophus phlebodes, Incilius</taxonomicName>
valliceps, Leptodactylus melanonotus, Lithobates forreri, Lithobates vaillanti, Rhinella horribilis, 
<taxonomicName id="AA98AC8CD5494C40FAEDFCEBA575FCA0" baseAuthorityName="Cope" baseAuthorityYear="1887" class="Amphibia" family="Hylidae" genus="Scinax" kingdom="Animalia" order="Anura" pageId="19" pageNumber="520" phylum="Chordata" rank="species" species="boulengeri">Scinax boulengeri</taxonomicName>
, 
<taxonomicName id="AA98AC8CD5494C40FEA0FCB7A596FCA0" baseAuthorityName="Cope" baseAuthorityYear="1875" box="[287,496,835,861]" class="Amphibia" family="Hylidae" genus="Scinax" kingdom="Animalia" order="Anura" pageId="19" pageNumber="520" phylum="Chordata" rank="species" species="elaeochroa">Scinax elaeochroa</taxonomicName>
, 
<taxonomicName id="AA98AC8CD5494C40FE44FCB7A6BEFCA0" baseAuthorityName="Gaige &amp; Stuart" baseAuthorityYear="1934" box="[507,728,835,861]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="19" pageNumber="520" phylum="Chordata" rank="species" species="loquax">Tlalocohyla loquax</taxonomicName>
.
</emphasis>
All were confirmed to reproduce in the same wetland.
</paragraph>
<caption id="39E78787D5494C40FF27F8E0A10EF8AC" ID-DOI="http://doi.org/10.5281/zenodo.7037425" ID-Zenodo-Dep="7037425" httpUri="https://zenodo.org/record/7037425/files/figure.png" pageId="19" pageNumber="520" startId="19.[152,255,1812,1836]" targetBox="[170,1436,896,1789]" targetPageId="19">
<paragraph id="6D27D70FD5494C40FF27F8E0A10EF8AC" blockId="19.[151,1436,1812,1873]" pageId="19" pageNumber="520">
<emphasis id="5FEC0B1DD5494C40FF27F8E0A54FF8D1" bold="true" box="[152,297,1812,1836]" pageId="19" pageNumber="520">FIGURE 13.</emphasis>
Geographic distribution of the genus 
<taxonomicName id="AA98AC8CD5494C40FD7BF8E0A75BF8D1" box="[708,829,1812,1836]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="19" pageNumber="520" phylum="Chordata" rank="genus">
<emphasis id="5FEC0B1DD5494C40FD7BF8E0A75BF8D1" box="[708,829,1812,1836]" italics="true" pageId="19" pageNumber="520">Tlalocohyla</emphasis>
</taxonomicName>
in Mesoamerica. Inset, map of Costa Rica, showing the distribution of 
<taxonomicName id="AA98AC8CD5494C40FE91F8CCA594F8AD" baseAuthorityName="Gaige &amp; Stuart" baseAuthorityYear="1934" box="[302,498,1848,1872]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="19" pageNumber="520" phylum="Chordata" rank="species" species="loquax">
<emphasis id="5FEC0B1DD5494C40FE91F8CCA594F8AD" box="[302,498,1848,1872]" italics="true" pageId="19" pageNumber="520">Tlalocohyla loquax</emphasis>
</taxonomicName>
(green circles) and 
<taxonomicName id="AA98AC8CD5494C40FD04F8CCA7E6F8AD" authority="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack, 2022" authorityName="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack" authorityYear="2022" box="[699,896,1848,1872]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="19" pageNumber="520" phylum="Chordata" rank="species" species="celeste" status="sp. nov.">
<emphasis id="5FEC0B1DD5494C40FD04F8CCA7E6F8AD" box="[699,896,1848,1872]" italics="true" pageId="19" pageNumber="520">Tlalocohyla celeste</emphasis>
</taxonomicName>
<emphasis id="5FEC0B1DD5494C40FC39F8CDA7B0F8AC" bold="true" box="[902,982,1849,1873]" pageId="19" pageNumber="520">
<taxonomicNameLabel id="44DFB666D5494C40FC39F8CDA7B0F8AC" box="[902,982,1849,1873]" pageId="19" pageNumber="520" rank="species">sp. nov.</taxonomicNameLabel>
</emphasis>
(red circle). Sources: (GBIF.org 2022).
</paragraph>
</caption>
<paragraph id="6D27D70FD5494C47FF78F88AA0A8FF04" blockId="19.[151,1436,1918,2017]" lastBlockId="20.[151,1437,151,789]" lastPageId="20" lastPageNumber="521" pageId="19" pageNumber="520">
<emphasis id="5FEC0B1DD5494C40FF78F88AA6E2F865" bold="true" box="[199,644,1918,1945]" pageId="19" pageNumber="520">Geographic distribution and habitat.</emphasis>
<taxonomicName id="AA98AC8CD5494C40FD33F88BA700F864" authorityName="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack" authorityYear="2022" box="[652,870,1919,1945]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="19" pageNumber="520" phylum="Chordata" rank="species" species="celeste">
<emphasis id="5FEC0B1DD5494C40FD33F88BA700F864" box="[652,870,1919,1945]" italics="true" pageId="19" pageNumber="520">Tlalocohyla celeste</emphasis>
</taxonomicName>
<materialsCitation id="DDF0DD52D5494C40FCD0F88BA1FAF81C" ID-GBIF-Occurrence="3897296304" collectorName="The &amp; de Upala" country="Costa Rica" elevation="660" latitude="10.71" location="Tapir Valley Nature Reserve" longLatPrecision="778" longitude="-85.01" municipality="Bijagua de Upala" pageId="19" pageNumber="520" specimenCount="1" stateProvince="Alajuela">
is only known to inhabit a single wetland system in the privately owned 
<location id="684781D4D5494C40FE23F857A6BDF840" LSID="urn:lsid:plazi:treatment:E5316619D55E4C46FF28FCEBA642FE08:684781D4D5494C40FE23F857A6BDF840" box="[412,731,1955,1981]" country="Costa Rica" latitude="10.71" longLatPrecision="778" longitude="-85.01" municipality="Bijagua de Upala" name="Tapir Valley Nature Reserve" pageId="19" pageNumber="520" stateProvince="Alajuela">Tapir Valley Nature Reserve</location>
(Lat: 
<geoCoordinate id="08ACB1C8D5494C40FC9FF857A71DF840" box="[800,891,1955,1981]" degrees="10.71" direction="north" orientation="latitude" pageId="19" pageNumber="520" precision="555" value="10.71">10.71 N</geoCoordinate>
Long: - 
<geoCoordinate id="08ACB1C8D5494C40FC6BF856A05FF840" box="[980,1081,1954,1981]" degrees="85.01" direction="west" orientation="longitude" pageId="19" pageNumber="520" precision="555" value="-85.01">85.01 W</geoCoordinate>
; 
<elevation id="E6B5303CD5494C40FBFAF857A143F840" box="[1093,1317,1954,1981]" metricMagnitude="2" metricUnit="m" metricValue="6.6" pageId="19" pageNumber="520" unit="m" value="660.0">
elevation 
<quantity id="AA607AEAD5494C40FB0CF856A09DF840" box="[1203,1275,1954,1981]" metricMagnitude="2" metricUnit="m" metricValue="6.6" pageId="19" pageNumber="520" unit="m" value="660.0">660 m</quantity>
asl
</elevation>
). 
<collectorName id="C06DB2D9D5494C40FA86F857A103F840" box="[1337,1381,1955,1981]" pageId="19" pageNumber="520">The</collectorName>
<typeStatus id="B22369ADD5494C40FAD3F857A1FAF840" box="[1388,1436,1955,1981]" pageId="19" pageNumber="520">type</typeStatus>
locality is located 
<locationDeviation id="7C95CE2DD5494C40FED9F833A6B4F81C" box="[358,722,1991,2017]" firstLocation="Tenorio" pageId="19" pageNumber="520" secondLocation="Miravalles">between Tenorio and Miravalles</locationDeviation>
volcanoes, in 
<collectingMunicipality id="8D434D75D5494C40FCC9F833A058F81C" box="[886,1086,1991,2017]" pageId="19" pageNumber="520">
Bijagua 
<collectorName id="C06DB2D9D5494C40FC6AF833A058F81C" box="[981,1086,1991,2017]" pageId="19" pageNumber="520">de Upala</collectorName>
</collectingMunicipality>
, 
<collectingRegion id="AF5C19EDD5494C40FBF7F833A173F81C" box="[1096,1301,1991,2017]" country="Costa Rica" name="Alajuela" pageId="19" pageNumber="520">Alajuela Province</collectingRegion>
, 
<collectingCountry id="158F979FD5494C40FA9EF833A1FAF81C" box="[1313,1436,1991,2017]" name="Costa Rica" pageId="19" pageNumber="520">Costa Rica</collectingCountry>
</materialsCitation>
(
<figureCitation id="F5A3CB8AD54E4C47FF20FF63A490FF4C" box="[159,246,151,177]" captionStart="FIGURE 13" captionStartId="19.[152,255,1812,1836]" captionTargetBox="[170,1436,896,1789]" captionTargetId="figure-331@19.[151,1436,885,1789]" captionTargetPageId="19" captionText="FIGURE 13. Geographic distribution of the genus Tlalocohyla in Mesoamerica. Inset, map of Costa Rica, showing the distribution of Tlalocohyla loquax (green circles) and Tlalocohyla celeste sp. nov. (red circle). Sources: (GBIF.org 2022)." figureDoi="http://doi.org/10.5281/zenodo.7037425" httpUri="https://zenodo.org/record/7037425/files/figure.png" pageId="20" pageNumber="521">Fig. 13</figureCitation>
). The reserve’s life zones are classified as Tropical Premontane Wet Forest and Tropical Moist Forest (
<bibRefCitation id="0909AAFED54E4C47FF20FF4FA530FF28" author="Holdridge, L. R." box="[159,342,187,213]" pageId="20" pageNumber="521" refId="ref13200" refString="Holdridge, L. R. (1967) Life Zone Ecology. Tropical Science Center, San Jose, 206 pp." type="book" year="1967">Holdridge 1967</bibRefCitation>
). The number of dry months per year ranges from one to two, without a marked dry season. The mean annual precipitation is 
<quantity id="AA607AEAD54E4C47FE64FF2AA62BFF04" box="[475,589,222,249]" metricMagnitude="0" metricUnit="m" metricValue="3.5" pageId="20" pageNumber="521" unit="mm" value="3500.0">3,500 mm</quantity>
and monthly temperatures ranges 20–33 ºC (
<bibRefCitation id="0909AAFED54E4C47FBF9FF2BA0A7FF04" author="IMN &amp; Instituto Meteorologico Nacional" box="[1094,1217,223,249]" pageId="20" pageNumber="521" pagination="1998 - 2018" refId="ref13268" refString="IMN [Instituto Meteorologico Nacional] (2021) Mapas de Costa Rica, Datos Climaticos, Upala Periodo: 1998 - 2018. Available from https: // www. imn. ac. cr / mapa (accessed 23 November 2021)" type="book chapter" year="2021">IMN 2021</bibRefCitation>
).
</paragraph>
<paragraph id="6D27D70FD54E4C47FF78FEF7A173FDC0" blockId="20.[151,1437,151,789]" pageId="20" pageNumber="521">
The frogs inhabit an 8-hectare marsh with a seasonally fluctuating, but permanent water level. The wetland is continually fed by a small tributary, by rainwater collected from surrounding hillsides, and likely by groundwater that wells up from bottom springs.A narrow discharge channel facilitates slow, directional water movement through the wetland. In the past, sections of this wetland have been channelized for irrigation purposes but it is now reverting to a more natural state. The surrounding hills and foothills of nearby mountains are largely forested, but areas of cultivated grassland are interspersed throughout the region. The shallow part of the marsh’s littoral zone is dominated by the grass 
<taxonomicName id="AA98AC8CD54E4C47FE1FFE2FA704FE08" box="[416,866,475,501]" class="Liliopsida" family="Cyperaceae" genus="Rhynchospora" kingdom="Plantae" order="Poales" pageId="20" pageNumber="521" phylum="Tracheophyta" rank="species" species="corymbosa">
<emphasis id="5FEC0B1DD54E4C47FE1FFE2FA6A5FE08" box="[416,707,475,501]" italics="true" pageId="20" pageNumber="521">Rhynchospora corymbosa</emphasis>
(Cyperaceae)
</taxonomicName>
, whereas the riparian zone is occupied by a diverse assemblage of herbaceous vegetation (
<figureCitation id="F5A3CB8AD54E4C47FDF5FE0BA6F9FDE4" box="[586,671,511,537]" captionStart="FIGURE 14" captionStartId="20.[152,255,1765,1789]" captionTargetBox="[151,1436,813,1742]" captionTargetId="figure-328@20.[151,1436,813,1742]" captionTargetPageId="20" captionText="FIGURE 14. Habitat where Tlalocohyla celeste sp. nov. is found. General view of the north (A) and southwest (B) sector of the wetland. The habitat is dominated by the grass Rhynchospora corymbosa (C), but other species of plants tolerant to flooded environments can be used by the frog for its reproductive activity (D)." figureDoi="http://doi.org/10.5281/zenodo.7037427" httpUri="https://zenodo.org/record/7037427/files/figure.png" pageId="20" pageNumber="521">Fig. 14</figureCitation>
). Away from the water’s edge, the vegetation structure changes and herbaceous plants are replaced by woody shrubs and trees, which connect to forest in the upland areas.
</paragraph>
<paragraph id="6D27D70FD54E4C47FF78FDB3A661FCE8" blockId="20.[151,1437,151,789]" pageId="20" pageNumber="521">
<taxonomicName id="AA98AC8CD54E4C47FF78FDB3A5F8FD9C" authorityName="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack" authorityYear="2022" box="[199,414,583,609]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="20" pageNumber="521" phylum="Chordata" rank="species" species="celeste">
<emphasis id="5FEC0B1DD54E4C47FF78FDB3A5F8FD9C" box="[199,414,583,609]" italics="true" pageId="20" pageNumber="521">Tlalocohyla celeste</emphasis>
</taxonomicName>
is an abundant species within the marsh at Tapir Valley Reserve. During periods of increased activity their calls can be heard throughout the littoral zone of the wetland, and one can readily observe up to 50 individuals in a single night. Long-term monitoring is ongoing to determine the population dynamics of this species. In spite of its local abundance, repeated visual and acoustic searches in nearby wetlands within a 
<quantity id="AA607AEAD54E4C47FAAEFD47A12BFD30" box="[1297,1357,691,717]" metricMagnitude="3" metricUnit="m" metricValue="2.0" pageId="20" pageNumber="521" unit="km" value="2.0">2 km</quantity>
radius that seemingly displayed similar habitat quality and characteristics have thus far not resulted in the discovery of additional 
<taxonomicName id="AA98AC8CD54E4C47FEB1FD0FA510FCE8" authorityName="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack" authorityYear="2022" box="[270,374,763,789]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="20" pageNumber="521" phylum="Chordata" rank="species" species="celeste">
<emphasis id="5FEC0B1DD54E4C47FEB1FD0FA510FCE8" box="[270,374,763,789]" italics="true" pageId="20" pageNumber="521">T. celeste</emphasis>
</taxonomicName>
populations.
</paragraph>
<caption id="39E78787D54E4C47FF27F911A738F8BB" ID-DOI="http://doi.org/10.5281/zenodo.7037427" ID-Zenodo-Dep="7037427" httpUri="https://zenodo.org/record/7037427/files/figure.png" pageId="20" pageNumber="521" startId="20.[152,255,1765,1789]" targetBox="[151,1436,813,1742]" targetPageId="20">
<paragraph id="6D27D70FD54E4C47FF27F911A738F8BB" blockId="20.[151,1436,1765,1862]" pageId="20" pageNumber="521">
<emphasis id="5FEC0B1DD54E4C47FF27F911A540F903" bold="true" box="[152,294,1765,1790]" pageId="20" pageNumber="521">FIGURE 14.</emphasis>
Habitat where 
<taxonomicName id="AA98AC8CD54E4C47FE7BF912A6EFF903" authority="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack, 2022" authorityName="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack" authorityYear="2022" box="[452,649,1766,1790]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="20" pageNumber="521" phylum="Chordata" rank="species" species="celeste" status="sp. nov.">
<emphasis id="5FEC0B1DD54E4C47FE7BF912A6EFF903" box="[452,649,1766,1790]" italics="true" pageId="20" pageNumber="521">Tlalocohyla celeste</emphasis>
</taxonomicName>
<emphasis id="5FEC0B1DD54E4C47FD2FF912A687F903" bold="true" box="[656,737,1766,1790]" pageId="20" pageNumber="521">
<taxonomicNameLabel id="44DFB666D54E4C47FD2FF912A687F903" box="[656,737,1766,1790]" pageId="20" pageNumber="521" rank="species">sp. nov.</taxonomicNameLabel>
</emphasis>
is found. General view of the north (A) and southwest (B) sector of the wetland. The habitat is dominated by the grass 
<taxonomicName id="AA98AC8CD54E4C47FD27F8FEA7F9F8DF" box="[664,927,1802,1826]" class="Liliopsida" family="Cyperaceae" genus="Rhynchospora" kingdom="Plantae" order="Poales" pageId="20" pageNumber="521" phylum="Tracheophyta" rank="species" species="corymbosa">
<emphasis id="5FEC0B1DD54E4C47FD27F8FEA7F9F8DF" box="[664,927,1802,1826]" italics="true" pageId="20" pageNumber="521">Rhynchospora corymbosa</emphasis>
</taxonomicName>
(C), but other species of plants tolerant to flooded environments can be used by the frog for its reproductive activity (D).
</paragraph>
</caption>
<paragraph id="6D27D70FD54E4C46FF78F880A5F8FF28" blockId="20.[151,1436,1908,2042]" lastBlockId="21.[151,1437,151,501]" lastPageId="21" lastPageNumber="522" pageId="20" pageNumber="521">
<emphasis id="5FEC0B1DD54E4C47FF78F880A5E3F873" bold="true" box="[199,389,1908,1934]" pageId="20" pageNumber="521">Natural history.</emphasis>
Individuals of this new species have been observed catching small flies and micro moths, and presumably its diet consists of a variety of small invertebrates. Although no direct observations of predation on adult 
<taxonomicName id="AA98AC8CD54E4C47FF28F849A499F82B" authorityName="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack" authorityYear="2022" box="[151,255,1980,2006]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="20" pageNumber="521" phylum="Chordata" rank="species" species="celeste">
<emphasis id="5FEC0B1DD54E4C47FF28F849A499F82B" box="[151,255,1980,2006]" italics="true" pageId="20" pageNumber="521">T. celeste</emphasis>
</taxonomicName>
were made, its wetland habitat harbors abundant snakes that are known predators of anurans. In addition, ctenid spiders, which are common frog predators, abound. During the course of our surveys, opilionid arachnids were observed scavenging a dead frog and small wasps were seen attacking 
<taxonomicName id="AA98AC8CD54F4C46FC45FF63A004FF4C" authorityName="Varela-Soto &amp; Abarca &amp; Brenes-Mora &amp; Aspinall &amp; Leenders &amp; Shepack" authorityYear="2022" box="[1018,1122,151,177]" class="Amphibia" family="Hylidae" genus="Tlalocohyla" kingdom="Animalia" order="Anura" pageId="21" pageNumber="522" phylum="Chordata" rank="species" species="celeste">
<emphasis id="5FEC0B1DD54F4C46FC45FF63A004FF4C" box="[1018,1122,151,177]" italics="true" pageId="21" pageNumber="522">T. celeste</emphasis>
</taxonomicName>
egg masses and preying on early developing larvae
</paragraph>
</subSubSection>
<subSubSection id="25828484D54F4C46FF78FF2BA642FE08" pageId="21" pageNumber="522" type="etymology">
<paragraph id="6D27D70FD54F4C46FF78FF2BA642FE08" blockId="21.[151,1437,151,501]" pageId="21" pageNumber="522">
<emphasis id="5FEC0B1DD54F4C46FF78FF2BA528FF04" bold="true" box="[199,334,223,249]" pageId="21" pageNumber="522">Etymology.</emphasis>
The specific epithet “celeste” (light blue, or sky-blue in Spanish), is used as a noun in apposition, and refers to the striking blue coloration seen on this frog’s axillary membranes and groin area, as well as on the inflated vocal sac in adult males. Perhaps more importantly, the authors want to celebrate the river Río Celeste, famous for the distinctive turquoise-blue hue of its water, whose watershed feeds the wetlands that provide critical habitat for its namesake frog. Río Celeste represents a critical natural resource and an important economic driver for local communities, as well as a nexus for sharing the beauty of nature with visitors from outside the region. The fact that this frog was discovered through local wetland restoration efforts is a testament to the critical importance of protecting our aquatic resources.
</paragraph>
</subSubSection>
</treatment>
</document>