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<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.60.38040" ID-GBIF-Dataset="65d938c8-cdd5-47f8-803b-bfcd4920bd64" ID-PMC="PMC6879665" ID-Pensoft-Pub="1314-4049-60-69" ID-Pensoft-UUID="858D1D355F5851868AFCE4A14F1394B8" ID-PubMed="31798310" ModsDocID="1314-4049-60-69" checkinTime="1574358804276" checkinUser="pensoft" docAuthor="Wei, De-Ping, Wanasinghe, Dhanushka N., Hyde, Kevin D., Mortimer, Peter E., Xu, Jianchu, Xiao, Yuan-Pin, Bhunjun, Chitrabhanu S. &amp; To-anun, Chaiwat" docDate="2019" docId="08A339019124591088431D5824EDF08F" docLanguage="en" docName="MycoKeys 60: 69-92" docOrigin="MycoKeys 60" docSource="http://dx.doi.org/10.3897/mycokeys.60.38040" docTitle="Ophiocordyceps unilateralis Petch, Trans. Br. mycol. Soc. 16 (1): 74 1931" docType="treatment" docVersion="4" id="858D1D355F5851868AFCE4A14F1394B8" lastPageNumber="69" masterDocId="858D1D355F5851868AFCE4A14F1394B8" masterDocTitle="The genus Simplicillium" masterLastPageNumber="92" masterPageNumber="69" pageNumber="69" updateTime="1668136349009" updateUser="ExternalLinkService">
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<mods:titleInfo>
<mods:title>The genus Simplicillium</mods:title>
</mods:titleInfo>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Wei, De-Ping</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Wanasinghe, Dhanushka N.</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Hyde, Kevin D.</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Mortimer, Peter E.</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Xu, Jianchu</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Xiao, Yuan-Pin</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Bhunjun, Chitrabhanu S.</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>To-anun, Chaiwat</mods:namePart>
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<mods:typeOfResource>text</mods:typeOfResource>
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<mods:titleInfo>
<mods:title>MycoKeys</mods:title>
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<mods:part>
<mods:date>2019</mods:date>
<mods:detail type="volume">
<mods:number>60</mods:number>
</mods:detail>
<mods:extent unit="page">
<mods:start>69</mods:start>
<mods:end>92</mods:end>
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<mods:url>http://dx.doi.org/10.3897/mycokeys.60.38040</mods:url>
</mods:location>
<mods:classification>journal article</mods:classification>
<mods:identifier type="DOI">http://dx.doi.org/10.3897/mycokeys.60.38040</mods:identifier>
<mods:identifier type="Pensoft-Pub">1314-4049-60-69</mods:identifier>
<mods:identifier type="Pensoft-UUID">858D1D355F5851868AFCE4A14F1394B8</mods:identifier>
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<treatment ID-GBIF-Taxon="160511190" LSID="urn:lsid:plazi:treatment:08A339019124591088431D5824EDF08F" httpUri="http://treatment.plazi.org/id/08A339019124591088431D5824EDF08F" lastPageNumber="69" pageId="0" pageNumber="69">
<subSubSection pageId="0" pageNumber="69" type="nomenclature">
<paragraph pageId="0" pageNumber="69">
<taxonomicName LSID="08A33901-9124-5910-8843-1D5824EDF08F" authority="Tul. &amp; C. Tul.) Petch, Trans. Br. mycol. Soc. 16 (1): 74 (1931" authorityYear="1931" class="Sordariomycetes" family="Cordycipitaceae" genus="Ophiocordyceps" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Ophiocordyceps unilateralis" order="Hypocreales" pageId="0" pageNumber="69" phylum="Ascomycota" rank="species" species="unilateralis">Ophiocordyceps unilateralis (Tul. &amp; C. Tul.) Petch, Trans. Br. mycol. Soc. 16(1): 74 (1931)</taxonomicName>
<figureCitation captionStart="Figure 6" captionStartId="F6" captionText="Figure 6. Ophiocordyceps unilateralis (from HKAS 102447) a stroma emerging from host b ascomata on stroma c host (Formicidae) d, e decayed perithecia. Scale bars: 500 µm (b, c); 300 µm (d); 100 µm (e)." figureDoi="10.3897/mycokeys.60.38040.figure6" httpUri="https://binary.pensoft.net/fig/357986" pageId="0" pageNumber="69" tableDoi="10.3897/mycokeys.60.38040.figure6">Figure 6</figureCitation>
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="69" type="description">
<paragraph pageId="0" pageNumber="69">Description.</paragraph>
<paragraph pageId="0" pageNumber="69">
Parasitic on ants (
<taxonomicName class="Insecta" family="Formicidae" genus="Formicidae" higherTaxonomySource="GBIF" kingdom="Animalia" lsidName="Formicidae" order="Hymenoptera" pageId="0" pageNumber="69" phylum="Arthropoda" rank="genus">
<emphasis italics="true" pageId="0" pageNumber="69">Formicidae</emphasis>
</taxonomicName>
).
<emphasis bold="true" italics="true" pageId="0" pageNumber="69">Sexual morph</emphasis>
:
<emphasis italics="true" pageId="0" pageNumber="69">Stromata</emphasis>
up to 14 mm in length, 0.5 mm wide in the broadest part, cylindrical, brown, slightly tapering towards the apex, single, piercing through the dorsal neck region of the ant host.
<emphasis italics="true" pageId="0" pageNumber="69">Ascomatal cushion</emphasis>
hemisphere, up to 1.2 mm in diam., laterally attaching to the erect stroma stalk, dark brown, with ostioles protruding from the cushions.
<emphasis italics="true" pageId="0" pageNumber="69">Perithecia</emphasis>
200-400
<normalizedToken originalValue="×">x</normalizedToken>
50-120 (xˉ = 294
<normalizedToken originalValue="×">x</normalizedToken>
81, n = 10)
<normalizedToken originalValue="µm">µm</normalizedToken>
, sub-immersed, flask-shaped.
<emphasis italics="true" pageId="0" pageNumber="69">Asci</emphasis>
and ascospores were too old to observe their features.
<emphasis bold="true" italics="true" pageId="0" pageNumber="69">Asexual morph</emphasis>
: Undetermined.
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="69" type="notes">
<paragraph pageId="0" pageNumber="69">Note.</paragraph>
<paragraph pageId="0" pageNumber="69">
This collection was already decayed and was colonised by other fungi which we introduced as a new host record of
<taxonomicName class="Sordariomycetes" family="Cordycipitaceae" genus="Simplicillium" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Simplicillium lanosoniveum" order="Hypocreales" pageId="0" pageNumber="69" phylum="Ascomycota" rank="species" species="lanosoniveum">
<emphasis italics="true" pageId="0" pageNumber="69">Simplicillium lanosoniveum</emphasis>
</taxonomicName>
from Thailand. The outline of this specimen was intact, while its asci and ascospores were too old to analyse. We retrieved DNA through direct sequencing from the stromal tissue.
</paragraph>
<paragraph pageId="0" pageNumber="69">
Sequences generated from this specimen have been deposited in GenBank with accession numbers: SSU = MK752759, LSU = MK752812, ITS = MK752874. The herbarium material is deposited at KUN herbarium, Yunnan Province, China. In the multi-gene phylogenetic tree, this collection groups with
<taxonomicName class="Sordariomycetes" family="Ophiocordycipitaceae" genus="Ophiocordyceps" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Ophiocordyceps unilateralis" order="Hypocreales" pageId="0" pageNumber="69" phylum="Ascomycota" rank="species" species="unilateralis">
<emphasis italics="true" pageId="0" pageNumber="69">Ophiocordyceps unilateralis</emphasis>
</taxonomicName>
(OSC 128574) with a strongly supported bootstrap value (100% ML, 1.00 BYPP, 100% MP,
<figureCitation captionStart="Figure 1" captionStartId="F1" captionText="Figure 1. Phylogram generated from maximum likelihood analysis based on combined SSU, LSU, TEF and RPB 1 sequence data. Bootstrap values for maximum likelihood (ML, left) and maximum parsimony (MP, right) equal to or greater than 50 % and Bayesian posterior probabilities (BYPP, middle) equal to or greater than 0.90 are placed nearby the note. The newly generated sequences are indicated in red bold." figureDoi="10.3897/mycokeys.60.38040.figure1" httpUri="https://binary.pensoft.net/fig/357981" pageId="0" pageNumber="69" tableDoi="10.3897/mycokeys.60.38040.figure1">Figure 1</figureCitation>
). Therefore, we identify this collection as
<taxonomicName lsidName="O. unilateralis" pageId="0" pageNumber="69" rank="species" species="unilateralis">
<emphasis italics="true" pageId="0" pageNumber="69">O. unilateralis</emphasis>
</taxonomicName>
, based on its morphologic features and molecular evidence.
</paragraph>
<caption doi="10.3897/mycokeys.60.38040.figure6" httpUri="https://binary.pensoft.net/fig/357986" pageId="0" pageNumber="69" start="Figure 6" startId="F6">
<paragraph pageId="0" pageNumber="69">
<emphasis bold="true" pageId="0" pageNumber="69">Figure 6.</emphasis>
<taxonomicName class="Sordariomycetes" family="Ophiocordycipitaceae" genus="Ophiocordyceps" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Ophiocordyceps unilateralis" order="Hypocreales" pageId="0" pageNumber="69" phylum="Ascomycota" rank="species" species="unilateralis">
<emphasis italics="true" pageId="0" pageNumber="69">Ophiocordyceps unilateralis</emphasis>
</taxonomicName>
(from HKAS 102447)
<emphasis bold="true" pageId="0" pageNumber="69">a</emphasis>
stroma emerging from host
<emphasis bold="true" pageId="0" pageNumber="69">b</emphasis>
ascomata on stroma
<emphasis bold="true" pageId="0" pageNumber="69">c</emphasis>
host (
<taxonomicName class="Insecta" family="Formicidae" genus="Formicidae" higherTaxonomySource="GBIF" kingdom="Animalia" lsidName="Formicidae" order="Hymenoptera" pageId="0" pageNumber="69" phylum="Arthropoda" rank="genus">Formicidae</taxonomicName>
)
<emphasis bold="true" pageId="0" pageNumber="69">d, e</emphasis>
decayed perithecia. Scale bars: 500
<normalizedToken originalValue="µm">µm</normalizedToken>
(
<emphasis bold="true" pageId="0" pageNumber="69">b, c</emphasis>
); 300
<normalizedToken originalValue="µm">µm</normalizedToken>
(
<emphasis bold="true" pageId="0" pageNumber="69">d</emphasis>
); 100µm (
<emphasis bold="true" pageId="0" pageNumber="69">e</emphasis>
).
</paragraph>
</caption>
</subSubSection>
</treatment>
</document>