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<document ID-DOI="http://dx.doi.org/10.3897/BDJ.9.e70446" ID-PMC="PMC8410753" ID-Pensoft-Pub="1314-2828-9-e70446" ID-Pensoft-UUID="1679FAD91AB65DC58F5E03B4526D6DAE" ID-PubMed="34539202" ModsDocID="1314-2828-9-e70446" checkinTime="1629994747510" checkinUser="pensoft" docAuthor="Yang, Qi, Zeng, Xiang-Yu, Yuan, Jun, Zhang, Qian, He, Yu-Ke &amp; Wang, Yong" docDate="2021" docId="A0E690E4DC7E5D2FA2280FCD3E9966DB" docLanguage="en" docName="BiodivDatJour 9: e70446" docOrigin="Biodiversity Data Journal 9" docPubDate="2021-08-25" docSource="http://dx.doi.org/10.3897/BDJ.9.e70446" docTitle="Neopestalotiopsis rhododendri Qi Yang &amp; Yong Wang bis 2021, sp. nov." docType="treatment" docVersion="2" id="1679FAD91AB65DC58F5E03B4526D6DAE" lastPageNumber="70446" masterDocId="1679FAD91AB65DC58F5E03B4526D6DAE" masterDocTitle="Two new species of Neopestalotiopsis from southern China" masterLastPageNumber="70446" masterPageNumber="70446" pageNumber="70446" updateTime="1668128823983" updateUser="ExternalLinkService">
<mods:mods xmlns:mods="http://www.loc.gov/mods/v3">
<mods:titleInfo>
<mods:title>Two new species of Neopestalotiopsis from southern China</mods:title>
</mods:titleInfo>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Yang, Qi</mods:namePart>
<mods:affiliation>Department of Plant Pathology, Agricultural College, Guizhou University, Guiyang, China</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Zeng, Xiang-Yu</mods:namePart>
<mods:nameIdentifier type="ORCID">https://orcid.org/0000-0003-1341-1004</mods:nameIdentifier>
<mods:affiliation>Department of Plant Pathology, Agricultural College, Guizhou University, Guiyang, China</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Yuan, Jun</mods:namePart>
<mods:affiliation>Department of Plant Pathology, Agricultural College, Guizhou University, Guiyang, China</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Zhang, Qian</mods:namePart>
<mods:affiliation>Department of Plant Pathology, Agricultural College, Guizhou University, Guiyang, China</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>He, Yu-Ke</mods:namePart>
<mods:affiliation>Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai, Thailand</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Wang, Yong</mods:namePart>
<mods:affiliation>Department of Plant Pathology, Agricultural College, Guizhou University, Guiyang, China</mods:affiliation>
<mods:nameIdentifier type="email">yongwangbis@aliyun.com</mods:nameIdentifier>
</mods:name>
<mods:typeOfResource>text</mods:typeOfResource>
<mods:relatedItem type="host">
<mods:titleInfo>
<mods:title>Biodiversity Data Journal</mods:title>
</mods:titleInfo>
<mods:part>
<mods:date>2021</mods:date>
<mods:detail type="pubDate">
<mods:number>2021-08-25</mods:number>
</mods:detail>
<mods:detail type="volume">
<mods:number>9</mods:number>
</mods:detail>
<mods:extent unit="page">
<mods:start>70446</mods:start>
<mods:end>70446</mods:end>
</mods:extent>
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</mods:relatedItem>
<mods:location>
<mods:url>http://dx.doi.org/10.3897/BDJ.9.e70446</mods:url>
</mods:location>
<mods:classification>journal article</mods:classification>
<mods:identifier type="DOI">http://dx.doi.org/10.3897/BDJ.9.e70446</mods:identifier>
<mods:identifier type="Pensoft-Pub">1314-2828-9-e70446</mods:identifier>
<mods:identifier type="Pensoft-UUID">1679FAD91AB65DC58F5E03B4526D6DAE</mods:identifier>
</mods:mods>
<treatment LSID="urn:lsid:plazi:treatment:A0E690E4DC7E5D2FA2280FCD3E9966DB" httpUri="http://treatment.plazi.org/id/A0E690E4DC7E5D2FA2280FCD3E9966DB" lastPageNumber="70446" pageId="0" pageNumber="70446">
<subSubSection pageId="0" pageNumber="70446" type="nomenclature">
<paragraph pageId="0" pageNumber="70446">
<taxonomicName LSID="A0E690E4-DC7E-5D2F-A228-0FCD3E9966DB" authority="Qi Yang &amp; Yong Wang bis" authorityName="Qi Yang &amp; Yong Wang bis" authorityYear="2021" class="Sordariomycetes" family="Pestalotiopsidaceae" genus="Neopestalotiopsis" kingdom="Fungi" lsidName="Neopestalotiopsis rhododendri" order="Amphisphaeriales" pageId="0" pageNumber="70446" phylum="Ascomycota" rank="species" species="rhododendri" status="sp. nov.">Neopestalotiopsis rhododendri Qi Yang &amp; Yong Wang bis</taxonomicName>
<taxonomicNameLabel pageId="0" pageNumber="70446">sp. nov.</taxonomicNameLabel>
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="70446" type="materials_examined">
<paragraph pageId="0" pageNumber="70446">Materials</paragraph>
<paragraph pageId="0" pageNumber="70446">
<materialsCitation collectingDate="2021-01-01" collectingDateMax="2021-12-31" collectingDateMin="2021-01-01" collectorName="Qi Yang" country="China" county="Occurrence" determinerName="Qi Yang" latitude="25.083334" location="Taxon" municipality="Qi Yang" pageId="0" pageNumber="70446" specimenCount="1" stateProvince="Yunnan" typeStatus="Holotype">
<emphasis bold="true" pageId="0" pageNumber="70446">Type status:</emphasis>
<materialsCitation collectingDate="2021-01-01" collectingDateMax="2021-12-31" collectingDateMin="2021-01-01" collectorName="Qi Yang" country="China" county="Occurrence" determinerName="Qi Yang" latitude="25.083334" location="Taxon" municipality="Qi Yang" specimenCount="134" stateProvince="Yunnan" typeStatus="Holotype">
<typeStatus pageId="0" pageNumber="70446">Holotype</typeStatus>
.
<emphasis bold="true" pageId="0" pageNumber="70446">
<collectingCounty>Occurrence</collectingCounty>
:
</emphasis>
recordedBy:
<collectorName pageId="0" pageNumber="70446">
<collectingMunicipality>Qi Yang</collectingMunicipality>
</collectorName>
; occurrenceID: GUCC 21504;
<emphasis bold="true" pageId="0" pageNumber="70446">
<location LSID="urn:lsid:plazi:treatment:A0E690E4DC7E5D2FA2280FCD3E9966DB:460BC0269F07409F2BD3A7A25D78B246" country="China" county="Occurrence" latitude="25.083334" municipality="Qi Yang" name="Taxon" stateProvince="Yunnan">Taxon</location>
:
</emphasis>
scientificName:
<location LSID="urn:lsid:plazi:treatment:A0E690E4DC7E5D2FA2280FCD3E9966DB:CD8D1F4CD83CDE66DC07F26E2F4957A8" country="China" county="Occurrence" latitude="25.083334" municipality="Qi Yang" name="Neopestalotiopsis" stateProvince="Yunnan">Neopestalotiopsis</location>
rhododendri; order:
<location LSID="urn:lsid:plazi:treatment:A0E690E4DC7E5D2FA2280FCD3E9966DB:0B239C73BA3FA400193C5DA3FDFAEEC9" country="China" county="Occurrence" latitude="25.083334" municipality="Qi Yang" name="Amphisphaeriales" stateProvince="Yunnan">Amphisphaeriales</location>
; family:
<location LSID="urn:lsid:plazi:treatment:A0E690E4DC7E5D2FA2280FCD3E9966DB:0D458238BBCF3828F2F1C0E672D8B533" country="China" county="Occurrence" latitude="25.083334" municipality="Qi Yang" name="Sporocadaceae" stateProvince="Yunnan">Sporocadaceae</location>
; genus:
<location LSID="urn:lsid:plazi:treatment:A0E690E4DC7E5D2FA2280FCD3E9966DB:9D6E6EEDFB74A9B2BA8D8EE2F48E9CD7" country="China" county="Occurrence" latitude="25.083334" municipality="Qi Yang" name="Neopestalotiopsis" stateProvince="Yunnan">Neopestalotiopsis</location>
;
<emphasis bold="true" pageId="0" pageNumber="70446">
<location LSID="urn:lsid:plazi:treatment:A0E690E4DC7E5D2FA2280FCD3E9966DB:95DACE739EEE441CBC76C48469593F3D" country="China" county="Occurrence" latitude="25.083334" municipality="Qi Yang" name="Location" stateProvince="Yunnan">Location</location>
:
</emphasis>
country:
<collectingCountry name="China" pageId="0" pageNumber="70446">China</collectingCountry>
; stateProvince:
<collectingRegion country="China" name="Yunnan">Yunnan</collectingRegion>
; locality:
<location LSID="urn:lsid:plazi:treatment:A0E690E4DC7E5D2FA2280FCD3E9966DB:CABDEED0C597D5DA8E7A9134EE7B3972" country="China" county="Occurrence" latitude="25.083334" municipality="Qi Yang" name="Kunming" pageId="0" pageNumber="70446" stateProvince="Yunnan">Kunming</location>
; verbatimCoordinates: 102°72' E,
<geoCoordinate degrees="25" direction="north" minutes="05" orientation="latitude" precision="925" value="25.083334">25°05' N</geoCoordinate>
;
<emphasis bold="true" pageId="0" pageNumber="70446">
<location LSID="urn:lsid:plazi:treatment:A0E690E4DC7E5D2FA2280FCD3E9966DB:74CDFEB9207E0C257C1937DFEAA530C7" country="China" county="Occurrence" latitude="25.083334" municipality="Qi Yang" name="Identification" stateProvince="Yunnan">Identification</location>
:
</emphasis>
identifiedBy:
<determinerName pageId="0" pageNumber="70446">
<location LSID="urn:lsid:plazi:treatment:A0E690E4DC7E5D2FA2280FCD3E9966DB:0ED70F72F1F3378EAD9745C9EB57504F" country="China" county="Occurrence" latitude="25.083334" municipality="Qi Yang" name="Qi Yang" stateProvince="Yunnan">Qi Yang</location>
</determinerName>
; dateIdentified: 2021;
<emphasis bold="true" pageId="0" pageNumber="70446">
<location LSID="urn:lsid:plazi:treatment:A0E690E4DC7E5D2FA2280FCD3E9966DB:B30CEA9D9904FF600CF44977B5B6DD0B" country="China" county="Occurrence" latitude="25.083334" municipality="Qi Yang" name="Record Level" stateProvince="Yunnan">Record Level</location>
:
</emphasis>
collectionID: HGUP
<specimenCount type="generic">
134
<materialsCitation collectingDate="2021-01-01" collectingDateMax="2021-12-31" collectingDateMin="2021-01-01" collectorName="Qi Yang" country="China" county="Occurrence" determinerName="Qi Yang" latitude="26.966667" location="Taxon" municipality="Qi Yang" pageId="0" pageNumber="70446" specimenCount="1" stateProvince="Guizhou" typeStatus="Other material">
<emphasis bold="true" pageId="0" pageNumber="70446">Type status:</emphasis>
<materialsCitation collectingDate="2021-01-01" collectingDateMax="2021-12-31" collectingDateMin="2021-01-01" collectorName="Qi Yang" country="China" county="Occurrence" determinerName="Qi Yang" latitude="26.966667" location="Taxon" municipality="Qi Yang" specimenCount="1" stateProvince="Guizhou" typeStatus="Other material">
<typeStatus pageId="0" pageNumber="70446">Other material</typeStatus>
.
<emphasis bold="true" pageId="0" pageNumber="70446">
<collectingCounty>Occurrence</collectingCounty>
:
</emphasis>
recordedBy:
<collectorName pageId="0" pageNumber="70446">
<collectingMunicipality>Qi Yang</collectingMunicipality>
</collectorName>
; occurrenceID: GUCC 21505;
<emphasis bold="true" pageId="0" pageNumber="70446">
<location LSID="urn:lsid:plazi:treatment:A0E690E4DC7E5D2FA2280FCD3E9966DB:5E9FB9CFA1E282979399A682F5A3F8B7" country="China" county="Occurrence" latitude="26.966667" municipality="Qi Yang" name="Taxon" stateProvince="Guizhou">Taxon</location>
:
</emphasis>
scientificName:
<location LSID="urn:lsid:plazi:treatment:A0E690E4DC7E5D2FA2280FCD3E9966DB:68E8DBFFE85BFF57E2B0AE21993F15DE" country="China" county="Occurrence" latitude="26.966667" municipality="Qi Yang" name="Neopestalotiopsis" stateProvince="Guizhou">Neopestalotiopsis</location>
rhododendri; order:
<location LSID="urn:lsid:plazi:treatment:A0E690E4DC7E5D2FA2280FCD3E9966DB:73B550EA7E2E9FBE2121AF3247C8E7F2" country="China" county="Occurrence" latitude="26.966667" municipality="Qi Yang" name="Amphisphaeriales" stateProvince="Guizhou">Amphisphaeriales</location>
; family:
<location LSID="urn:lsid:plazi:treatment:A0E690E4DC7E5D2FA2280FCD3E9966DB:FD370039945DCD4F98E7191BE61833B4" country="China" county="Occurrence" latitude="26.966667" municipality="Qi Yang" name="Sporocadaceae" stateProvince="Guizhou">Sporocadaceae</location>
; genus:
<location LSID="urn:lsid:plazi:treatment:A0E690E4DC7E5D2FA2280FCD3E9966DB:C6CD1653312B8FEF9EDA2AEABA087320" country="China" county="Occurrence" latitude="26.966667" municipality="Qi Yang" name="Neopestalotiopsis" stateProvince="Guizhou">Neopestalotiopsis</location>
;
<emphasis bold="true" pageId="0" pageNumber="70446">
<location LSID="urn:lsid:plazi:treatment:A0E690E4DC7E5D2FA2280FCD3E9966DB:DA7C22C4DDAF9CD629F5B9A9FBA32193" country="China" county="Occurrence" latitude="26.966667" municipality="Qi Yang" name="Location" stateProvince="Guizhou">Location</location>
:
</emphasis>
country:
<collectingCountry name="China" pageId="0" pageNumber="70446">China</collectingCountry>
; stateProvince:
<collectingRegion country="China" name="Guizhou">Guizhou</collectingRegion>
; locality:
<location LSID="urn:lsid:plazi:treatment:A0E690E4DC7E5D2FA2280FCD3E9966DB:364DED7286EAED7FD3B4EF99E99AC3DA" country="China" county="Occurrence" latitude="26.966667" municipality="Qi Yang" name="Kaili" pageId="0" pageNumber="70446" stateProvince="Guizhou">Kaili</location>
; verbatimCoordinates: 107°97' E,
<geoCoordinate degrees="26" direction="north" minutes="58" orientation="latitude" precision="925" value="26.966667">26°58' N</geoCoordinate>
;
<emphasis bold="true" pageId="0" pageNumber="70446">Identification:</emphasis>
identifiedBy:
<determinerName pageId="0" pageNumber="70446">Qi Yang</determinerName>
; dateIdentified: 2021;
<emphasis bold="true" pageId="0" pageNumber="70446">Record Level:</emphasis>
collectionID: HGUP 997
</materialsCitation>
</materialsCitation>
</specimenCount>
</materialsCitation>
</materialsCitation>
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="70446" type="description">
<paragraph pageId="0" pageNumber="70446">Description</paragraph>
<paragraph pageId="0" pageNumber="70446">
<emphasis bold="true" pageId="0" pageNumber="70446">Disease symptom</emphasis>
: Associated with leaf spots of
<taxonomicName class="Magnoliopsida" family="Ericaceae" genus="Rhododendron" kingdom="Plantae" lsidName="Rhododendron simsii" order="Ericales" pageId="0" pageNumber="70446" phylum="Tracheophyta" rank="species" species="simsii">
<emphasis italics="true" pageId="0" pageNumber="70446">Rhododendron simsii</emphasis>
</taxonomicName>
. The leaf spots are small irregular to subcircular shape, brown, slightly sunken spots appear on surface leaves of
<taxonomicName lsidName="R. simsii" pageId="0" pageNumber="70446" rank="species" species="simsii">
<emphasis italics="true" pageId="0" pageNumber="70446">R. simsii</emphasis>
</taxonomicName>
, which scattered on the surface leaves tip and eventually develops into a large lesion. Small off-white spots appeared initially and then gradually enlarged, changing to light brown circular ring spots with a dark brown border.
</paragraph>
<paragraph pageId="0" pageNumber="70446">
<emphasis italics="true" pageId="0" pageNumber="70446">Colonies</emphasis>
on PDA reaching 6.5-7 cm in diam. after 7 d at room temperature (28°C), under light 12 hr/dark.
<emphasis italics="true" pageId="0" pageNumber="70446">Hyphae</emphasis>
white, colonies filamentous to circular, slightly undulate at the edge, with black fruiting bodies clustered, has filiform and fluffy margin, white from above and light yellow from the reverse. Sexual morph: undetermined. Asexual morphFig.
<figureCitation captionStart="Figure 2" captionStartId="F7164274" captionText="Figure 2. Neopestalotiopsis rhododendri (GUCC 21504). a, b, c. Leaf spots of Neopestalotiopsis rhododendri; d, e. Culture on PDA (d - above, e - reverse); f. Colony sporulating on PDA; g-h. Conidia and conidiophores; i-m. Conidia. Scale bars: f = 1000 µm, g-m = 20 µm." figureDoi="10.3897/BDJ.9.e70446.figure2" httpUri="https://binary.pensoft.net/fig/553722" pageId="0" pageNumber="70446">2</figureCitation>
:
<emphasis italics="true" pageId="0" pageNumber="70446">Conidiomata</emphasis>
55-280
<normalizedToken originalValue="µm">µm</normalizedToken>
in diam., pycnidial, globose, solitary, black, semi-immersed on PDA, exuding brown to dark brown mass of conidia.
<emphasis italics="true" pageId="0" pageNumber="70446">Conidiophores</emphasis>
often reduced to conidiogenous cell, regularly septate and branched at the base.
<emphasis italics="true" pageId="0" pageNumber="70446">Conidiogenous cells</emphasis>
mostly integrated, ampulliform, cylindrical, hyaline to light brown, smooth-walled.
<emphasis italics="true" pageId="0" pageNumber="70446">Conidia</emphasis>
(25.5-)30
<normalizedToken originalValue="×">x</normalizedToken>
5(-6)
<normalizedToken originalValue="µm">µm</normalizedToken>
(x̄ = 27.6
<normalizedToken originalValue="×">x</normalizedToken>
5.5
<normalizedToken originalValue="µm">µm</normalizedToken>
, n = 30), fusiform to clavate, straight to slightly curved, 4-septate; basal cell obconic, hyaline, thin-walled, smooth, 3.5-6.5
<normalizedToken originalValue="µm">µm</normalizedToken>
(x̄ = 4.5
<normalizedToken originalValue="µm">µm</normalizedToken>
, n = 30); the three median cells 13.5-19.5
<normalizedToken originalValue="µm">µm</normalizedToken>
(x̄ = 16.3
<normalizedToken originalValue="µm">µm</normalizedToken>
, n = 30), light brown to dark brown, dark brown with septa darker than the rest of the cells, the second cell from base 4-6
<normalizedToken originalValue="µm">µm</normalizedToken>
(x̄ = 5
<normalizedToken originalValue="µm">µm</normalizedToken>
, n = 30); the third cell 3.5-5.5
<normalizedToken originalValue="µm">µm</normalizedToken>
(x̄ = 4.5
<normalizedToken originalValue="µm">µm</normalizedToken>
, n = 30); the fourth cell 4-6.5
<normalizedToken originalValue="µm">µm</normalizedToken>
(x̄ = 4.8
<normalizedToken originalValue="µm">µm</normalizedToken>
, n = 30); apical cell 3.5-6.3
<normalizedToken originalValue="µm">µm</normalizedToken>
(x̄ = 5
<normalizedToken originalValue="µm">µm</normalizedToken>
, n = 30), cylindrical to sub-cylindrical, hyaline, 1-3 (mostly 2) tubular apical appendages, arising from the apex of the apical cell each at different points, 21-38.5
<normalizedToken originalValue="µm">µm</normalizedToken>
(x̄ = 29.2
<normalizedToken originalValue="µm">µm</normalizedToken>
, n = 30); basal appendage present most of the time, single, tubular, unbranched, 6-11.5
<normalizedToken originalValue="µm">µm</normalizedToken>
(x̄ = 8.5
<normalizedToken originalValue="µm">µm</normalizedToken>
, n = 30).
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="70446" type="etymology">
<paragraph pageId="0" pageNumber="70446">Etymology</paragraph>
<paragraph pageId="0" pageNumber="70446">
China, Yunnan Province, Kunming City, from leaves of
<taxonomicName class="Magnoliopsida" family="Ericaceae" genus="Rhododendron" kingdom="Plantae" lsidName="Rhododendron simsii" order="Ericales" pageId="0" pageNumber="70446" phylum="Tracheophyta" rank="species" species="simsii">
<emphasis italics="true" pageId="0" pageNumber="70446">Rhododendron simsii</emphasis>
</taxonomicName>
, 12 February 2018, Q. Zhang, HGUP 134, holotype, ex-type living culture GUCC 21504.
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="70446" type="notes">
<paragraph pageId="0" pageNumber="70446">Notes</paragraph>
<paragraph pageId="0" pageNumber="70446">
In the multi-gene analysis, strain GUCC 21504 formed a distinct clade with a sister strain GUCC 21505, but the node support values were 68/90/- (MP/ML/BI) and these two strains were close to
<taxonomicName lsidName="N. protearum" pageId="0" pageNumber="70446" rank="species" species="protearum">
<emphasis italics="true" pageId="0" pageNumber="70446">N. protearum</emphasis>
</taxonomicName>
(CBS 114178). When comparing the polymorphic nucleotide differences of our two strains, there are 18 base pair differences, seven in ITS, two in
<emphasis italics="true" pageId="0" pageNumber="70446">tub2</emphasis>
and nine in
<emphasis italics="true" pageId="0" pageNumber="70446">tef1</emphasis>
, but without obvious distinction (higher than 98.5%). Compared with
<taxonomicName lsidName="N. protearum" pageId="0" pageNumber="70446" rank="species" species="protearum">
<emphasis italics="true" pageId="0" pageNumber="70446">N. protearum</emphasis>
</taxonomicName>
and our ex-type strain (GUCC 21504), there were six character differences with
<taxonomicName lsidName="N. protearum" pageId="0" pageNumber="70446" rank="species" species="protearum">
<emphasis italics="true" pageId="0" pageNumber="70446">N. protearum</emphasis>
</taxonomicName>
in the ITS region, three character differences with
<taxonomicName lsidName="N. protearum" pageId="0" pageNumber="70446" rank="species" species="protearum">
<emphasis italics="true" pageId="0" pageNumber="70446">N. protearum</emphasis>
</taxonomicName>
in the
<emphasis italics="true" pageId="0" pageNumber="70446">tub2</emphasis>
region, but 12 character differences from
<taxonomicName lsidName="N. protearum" pageId="0" pageNumber="70446" rank="species" species="protearum">
<emphasis italics="true" pageId="0" pageNumber="70446">N. protearum</emphasis>
</taxonomicName>
in the
<emphasis italics="true" pageId="0" pageNumber="70446">tef1</emphasis>
region; thus the DNA base pair differences were mainly in the
<emphasis italics="true" pageId="0" pageNumber="70446">tef1</emphasis>
gene regions. The morphological differences between our strains and
<taxonomicName lsidName="N. protearum" pageId="0" pageNumber="70446" rank="species" species="protearum">
<emphasis italics="true" pageId="0" pageNumber="70446">N. protearum</emphasis>
</taxonomicName>
were wider conidia (
<taxonomicName lsidName="N. protearum" pageId="0" pageNumber="70446" rank="species" species="protearum">
<emphasis italics="true" pageId="0" pageNumber="70446">N. protearum</emphasis>
</taxonomicName>
: 24.8
<normalizedToken originalValue="±">+/-</normalizedToken>
1.5
<normalizedToken originalValue="×">x</normalizedToken>
8.5
<normalizedToken originalValue="±">+/-</normalizedToken>
0.6
<normalizedToken originalValue="µm">µm</normalizedToken>
), more apical appendages (
<taxonomicName lsidName="N. protearum" pageId="0" pageNumber="70446" rank="species" species="protearum">
<emphasis italics="true" pageId="0" pageNumber="70446">N. protearum</emphasis>
</taxonomicName>
: 3-5) and shorter basal appendages (
<taxonomicName lsidName="N. protearum" pageId="0" pageNumber="70446" rank="species" species="protearum">
<emphasis italics="true" pageId="0" pageNumber="70446">N. protearum</emphasis>
</taxonomicName>
: 5-8
<normalizedToken originalValue="µm">µm</normalizedToken>
) (
<bibRefCitation author="Maharachchikumbura, S S N" journalOrPublisher="Studies in Mycology" pageId="0" pageNumber="70446" pagination="121 - 186" refId="B7160118" refString="Maharachchikumbura, S S N, Hyde, K D, Groenewald, J Z, Xu, J, Crous, P W, 2014. Pestalotiopsis revisited. Studies in Mycology 79: 121 - 186" title="Pestalotiopsis revisited" volume="79" year="2014">Maharachchikumbura et al. 2014</bibRefCitation>
). Thus,
<taxonomicName authorityName="Qi Yang &amp; Yong Wang bis" authorityYear="2021" class="Sordariomycetes" family="Pestalotiopsidaceae" genus="Neopestalotiopsis" kingdom="Fungi" lsidName="Neopestalotiopsis rhododendri" order="Amphisphaeriales" pageId="0" pageNumber="70446" phylum="Ascomycota" rank="species" species="rhododendri">
<emphasis italics="true" pageId="0" pageNumber="70446">Neopestalotiopsis rhododendri</emphasis>
</taxonomicName>
is introduced as a novel taxon, based on morphology and phylogeny.
</paragraph>
</subSubSection>
</treatment>
</document>