treatments-xml/data/03/D2/87/03D287AE5B4CFFB77CF0F955FEFADA5F.xml

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<mods:title>A New Species Of Diploperla Needham And Claassen (Plecoptera: Perlodidae) From North Carolina And Virginia</mods:title>
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<mods:namePart>Kondratieff, Boris C.</mods:namePart>
<mods:affiliation>Department of Bioagricultural Sciences and Pest Management, Colorado State University, Fort Collins, Colorado, 80523, U. S. A. E-mail: boris. kondratieff @ colostate. edu E-mail: verdonec @ gmail. com</mods:affiliation>
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<mods:namePart>Verdone, Chris J.</mods:namePart>
<mods:affiliation>Department of Bioagricultural Sciences and Pest Management, Colorado State University, Fort Collins, Colorado, 80523, U. S. A. E-mail: boris. kondratieff @ colostate. edu E-mail: verdonec @ gmail. com</mods:affiliation>
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<taxonomicName authority="Kondratieff and Verdone" authorityName="Kondratieff and Verdone" authorityYear="2017" box="[194,721,1768,1792]" class="Insecta" family="Perlodidae" genus="Diploperla" kingdom="Animalia" order="Plecoptera" pageId="2" pageNumber="129" phylum="Arthropoda" rank="species" species="janeae" status="sp. n.">
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Kondratieff and Verdone
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http://lsid.speciesfile.org/urn:lsid: 
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.speciesfile.org: TaxonName:501063
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(
<figureCitation box="[335,425,1869,1894]" captionStart="Figs" captionStartId="1.[189,238,1156,1180]" captionTargetBox="[324,1307,254,1073]" captionTargetId="figure-320@1.[324,1307,254,1073]" captionTargetPageId="1" captionText="Figs. 1a–b. Adult habitus of Diploperla janeae sp. n., Helton Creek, Grayson Co., Virginia and D. duplicata, Laurel Fork, Floyd Co., Virginia, 1.5X. Fig. 1a. D. janeae sp. n, habitus, dorsal. Fig. 1b. D. duplicata, habitus, dorsal." figureDoi="http://doi.org/10.5281/zenodo.4757831" httpUri="https://zenodo.org/record/4757831/files/figure.png" pageId="2" pageNumber="129">Figs. 1a</figureCitation>
, 
<figureCitation box="[437,466,1870,1894]" captionStart="Figs" captionStartId="2.[189,238,865,889]" captionTargetBox="[286,1344,254,783]" captionTargetId="figure-424@2.[286,1344,254,783]" captionTargetPageId="2" captionText="Figs. 2a–b. Adult head and pronotum of Diploperla janeae sp. n., Helton Creek, Grayson Co., Virginia and D. duplicata, Bull Run Mtn, Prince William Co., Virginia, 5X. Fig. 2a. D. janeae sp. n, head and pronotum, dorsal. Fig. 2b. D. duplicata, head and pronotum, dorsal." figureDoi="http://doi.org/10.5281/zenodo.4757833" httpUri="https://zenodo.org/record/4757833/files/figure.png" pageId="2" pageNumber="129">2a</figureCitation>
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<figureCitation box="[477,492,1870,1894]" captionStart="Fig" captionStartId="3.[189,226,960,984]" captionTargetBox="[504,1128,254,878]" captionTargetId="figure-436@3.[504,1128,254,878]" captionTargetPageId="3" captionText="Fig. 3. Male sterna 6–10 of Diploperla janeae sp. n., Helton Creek, Grayson Co., Virginia, 5X." figureDoi="http://doi.org/10.5281/zenodo.4757837" httpUri="https://zenodo.org/record/4757837/files/figure.png" pageId="2" pageNumber="129">3</figureCitation>
, 
<figureCitation box="[504,532,1870,1894]" captionStart="Figs" captionStartId="4.[189,238,865,889]" captionTargetBox="[286,1344,254,783]" captionTargetId="figure-504@4.[286,1344,254,783]" captionTargetPageId="4" captionText="Figs. 4a–b. Terminalia of Diploperla janeae sp. n., Helton Creek, Grayson Co., Virginia and D. duplicata, Little Rock Castle Creek, Patrick Co., Virginia, 5X. E. Epiproct, HL. Hemitergal lobe, LS. Lateral stylet. Fig. 4a. D. janeae sp. n, terminalia and lateral stylet, dorsal expansion (arrow), lateral. Fig. 4b. D. duplicata, terminalia, lateral." figureDoi="http://doi.org/10.5281/zenodo.4757839" httpUri="https://zenodo.org/record/4757839/files/figure.png" pageId="2" pageNumber="129">4a</figureCitation>
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<figureCitation box="[544,572,1869,1893]" captionStart="Figs" captionStartId="5.[189,238,865,889]" captionTargetBox="[286,1344,254,783]" captionTargetId="figure-195@5.[286,1344,254,783]" captionTargetPageId="5" captionText="Figs. 5a–b. Epiproct of Diploperla janeae sp. n., Helton Creek, Grayson Co., Virginia and D. duplicata, Laurel Fork, Floyd Co., Virginia. Fig. 4a. D. janeae sp. n, epiproct, anterior, 1600X. Fig. 5b. D. duplicata, epiproct, lateral, 750X." figureDoi="http://doi.org/10.5281/zenodo.4757843" httpUri="https://zenodo.org/record/4757843/files/figure.png" pageId="2" pageNumber="129">5a</figureCitation>
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<figureCitation box="[584,613,1869,1893]" captionStart="Figs" captionStartId="5.[189,238,1611,1635]" captionTargetBox="[286,1344,999,1528]" captionTargetId="figure-197@5.[286,1344,999,1528]" captionTargetPageId="5" captionText="Figs. 6a–b. Paraprocts of Diploperla janeae sp. n., Helton Creek, Grayson Co., Virginia and D. duplicata, Laurel Fork, Floyd Co., Virginia. Fig. 6a. D. janeae sp. n, paraprocts and dorsal expansion (arrow), lateral, 430X. Fig. 6b. D. duplicata, paraprocts, lateral, 200X." figureDoi="http://doi.org/10.5281/zenodo.4757845" httpUri="https://zenodo.org/record/4757845/files/figure.png" pageId="2" pageNumber="129">6a</figureCitation>
, 
<figureCitation box="[624,651,1870,1894]" captionStart="Figs" captionStartId="7.[189,238,865,889]" captionTargetBox="[286,1344,254,783]" captionTargetId="figure-548@7.[286,1344,254,783]" captionTargetPageId="7" captionText="Figs. 7a–b. Female subgenital plate of Diploperla janeae sp. n., Helton Creek, Grayson Co., Virginia and D. duplicata, Little Rock Castle Creek, Patrick Co., Virginia, 5X. 7a. D. janeae sp. n, female subgenital plate. 7b. D. duplicata, female subgenital plate." figureDoi="http://doi.org/10.5281/zenodo.4757849" httpUri="https://zenodo.org/record/4757849/files/figure.png" pageId="2" pageNumber="129">7a</figureCitation>
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<paragraph blockId="2.[194,793,1804,1929]" box="[401,584,1905,1929]" pageId="2" pageNumber="129">Jane’s Stripetail</paragraph>
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<emphasis bold="true" box="[835,1068,1045,1069]" pageId="2" pageNumber="129">Material Examined</emphasis>
. 
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: 
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19E &amp; 
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at bridge, GSMNP, 
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Road &amp; Forest Road 711, 
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783, at gated trail by beaver pond, 
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<collectingDate box="[1187,1368,1585,1610]" pageId="2" pageNumber="129" value="2017-05-12">12 May 2017</collectingDate>
</date>
, 
<collectorName pageId="2" pageNumber="129">B.C. Kondratieff</collectorName>
, 
<collectorName box="[985,1121,1621,1645]" pageId="2" pageNumber="129">C. Verdone</collectorName>
, 
<specimenCount box="[1133,1153,1621,1645]" pageId="2" pageNumber="129" type="male">♂</specimenCount>
(
<collectionCode box="[1167,1253,1621,1645]" pageId="2" pageNumber="129">CSUIC</collectionCode>
)
</materialsCitation>
; 
<materialsCitation ID-GBIF-Occurrence="3111943324" collectingDate="2017-05-13" collectionCode="CSUIC" collectorName="B. C. Kondratieff &amp; C. Verdone" location="Same" pageId="2" pageNumber="129" specimenCount="3" specimenCount-female="1" specimenCount-male="2" stateProvince="Virginia" typeStatus="paratype">
<location LSID="urn:lsid:plazi:treatment:03D287AE5B4CFFB77CF0F955FEFADA5F:8EA460635B4CFFB978CAF9E8FACBD805" box="[1272,1335,1621,1645]" name="Same" pageId="2" pageNumber="129" stateProvince="Virginia">Same</location>
location, 
<date box="[835,991,1657,1682]" pageId="2" pageNumber="129" value="2017-05-13">
<collectingDate box="[835,991,1657,1682]" pageId="2" pageNumber="129" value="2017-05-13">13 May 2017</collectingDate>
</date>
, 
<collectorName box="[1006,1204,1657,1681]" pageId="2" pageNumber="129">B.C. Kondratieff</collectorName>
, 
<collectorName box="[1218,1358,1657,1681]" pageId="2" pageNumber="129">C. Verdone</collectorName>
, 
<specimenCount box="[1372,1407,1657,1681]" pageId="2" pageNumber="129" type="male">2♂</specimenCount>
, 
<specimenCount box="[1423,1443,1657,1681]" pageId="2" pageNumber="129" type="female">♀</specimenCount>
(
<collectionCode box="[843,930,1693,1717]" pageId="2" pageNumber="129">CSUIC</collectionCode>
)
</materialsCitation>
. 
<materialsCitation ID-GBIF-Occurrence="3111943403" collectingDate="2016-05-27" collectionCode="CSUIC" collectorName="C. Verdone &amp; B. C. Kondratieff" county="Tazewell Co." latitude="37.03494" location="Roaring Fork Rd." longLatPrecision="1" longitude="-81.47878" municipality="Roaring Fork" pageId="2" pageNumber="129" specimenCount="1" specimenCount-male="1" stateProvince="Virginia" typeStatus="paratype">
<emphasis bold="true" box="[948,1113,1693,1717]" pageId="2" pageNumber="129">
<collectingCounty box="[948,1106,1693,1717]" pageId="2" pageNumber="129">Tazewell Co.</collectingCounty>
,
</emphasis>
<collectingMunicipality box="[1119,1276,1693,1717]" pageId="2" pageNumber="129">Roaring Fork</collectingMunicipality>
, 
<location LSID="urn:lsid:plazi:treatment:03D287AE5B4CFFB77CF0F955FEFADA5F:8EA460635B4CFFB97935F920FC92D8B1" county="Tazewell Co." latitude="37.03494" longLatPrecision="1" longitude="-81.47878" municipality="Roaring Fork" name="Roaring Fork Rd." pageId="2" pageNumber="129" stateProvince="Virginia">Roaring Fork Rd.</location>
, 
<geoCoordinate box="[899,1041,1729,1753]" degrees="37.03494" direction="north" orientation="latitude" pageId="2" pageNumber="129" precision="1" value="37.03494">N 37.03494</geoCoordinate>
, 
<geoCoordinate box="[1061,1207,1729,1753]" degrees="81.47878" direction="west" orientation="longitude" pageId="2" pageNumber="129" precision="1" value="-81.47878">W 81.47878</geoCoordinate>
, 
<date box="[1227,1396,1729,1753]" pageId="2" pageNumber="129" value="2016-05-27">
<collectingDate box="[1227,1396,1729,1753]" pageId="2" pageNumber="129" value="2016-05-27">27 May 2016</collectingDate>
</date>
, 
<collectorName pageId="2" pageNumber="129">C. Verdone</collectorName>
, 
<collectorName box="[950,1144,1765,1789]" pageId="2" pageNumber="129">B.C. Kondratieff</collectorName>
, 
<specimenCount box="[1155,1175,1765,1789]" pageId="2" pageNumber="129" type="male">♂</specimenCount>
(
<collectionCode box="[1190,1277,1765,1789]" pageId="2" pageNumber="129">CSUIC</collectionCode>
)
</materialsCitation>
. 
<materialsCitation ID-GBIF-Occurrence="3111943369" collectingDate="2017-05-08" collectionCode="CSUIC" collectorName="B. C. Kondratieff &amp; C. Verdone" county="Co." latitude="36.68316" location="Forest Road" longLatPrecision="1" longitude="-81.64869" municipality="Straight Branch" pageId="2" pageNumber="129" specimenCount="1" specimenCount-female="1" stateProvince="Washington" typeStatus="paratype">
<emphasis bold="true" pageId="2" pageNumber="129">
<collectingRegion box="[1295,1443,1765,1789]" country="United States of America" name="Washington" pageId="2" pageNumber="129">Washington</collectingRegion>
<collectingCounty box="[835,876,1800,1824]" pageId="2" pageNumber="129">Co.</collectingCounty>
,
</emphasis>
<collectingMunicipality box="[890,1076,1801,1825]" pageId="2" pageNumber="129">Straight Branch</collectingMunicipality>
, 
<location LSID="urn:lsid:plazi:treatment:03D287AE5B4CFFB77CF0F955FEFADA5F:8EA460635B4CFFB97872F8B4FB32D949" box="[1088,1230,1801,1825]" county="Co." latitude="36.68316" longLatPrecision="1" longitude="-81.64869" municipality="Straight Branch" name="Forest Road" pageId="2" pageNumber="129" stateProvince="Washington">Forest Road</location>
837, 
<location LSID="urn:lsid:plazi:treatment:03D287AE5B4CFFB77CF0F955FEFADA5F:8EA460635B4CFFB9793EF8B4FA62D949" box="[1292,1438,1801,1825]" county="Co." latitude="36.68316" longLatPrecision="1" longitude="-81.64869" municipality="Straight Branch" name="Beaver Flats" pageId="2" pageNumber="129" stateProvince="Washington">Beaver Flats</location>
, 
<geoCoordinate box="[835,984,1837,1861]" degrees="36.68316" direction="north" orientation="latitude" pageId="2" pageNumber="129" precision="1" value="36.68316">N 36.68316</geoCoordinate>
, 
<geoCoordinate box="[1013,1166,1837,1861]" degrees="81.64869" direction="west" orientation="longitude" pageId="2" pageNumber="129" precision="1" value="-81.64869">W 81.64869</geoCoordinate>
, 
<date box="[1194,1365,1837,1862]" pageId="2" pageNumber="129" value="2017-05-08">
<collectingDate box="[1194,1365,1837,1862]" pageId="2" pageNumber="129" value="2017-05-08">8 May 2017</collectingDate>
</date>
, 
<collectorName pageId="2" pageNumber="129">B.C. Kondratieff</collectorName>
, 
<collectorName box="[985,1121,1873,1897]" pageId="2" pageNumber="129">C. Verdone</collectorName>
, 
<specimenCount box="[1133,1153,1873,1897]" pageId="2" pageNumber="129" type="female">♀</specimenCount>
(
<collectionCode box="[1167,1254,1873,1897]" pageId="2" pageNumber="129">CSUIC</collectionCode>
)
</materialsCitation>
.
</paragraph>
</subSubSection>
<caption ID-DOI="http://doi.org/10.5281/zenodo.4757837" ID-Zenodo-Dep="4757837" box="[189,1251,960,984]" httpUri="https://zenodo.org/record/4757837/files/figure.png" pageId="3" pageNumber="130" startId="3.[189,226,960,984]" targetBox="[504,1128,254,878]" targetPageId="3">
<paragraph blockId="3.[189,1251,960,984]" box="[189,1251,960,984]" pageId="3" pageNumber="130">
Fig. 3. Male sterna 6–10 of 
<taxonomicName authority="Kondratieff &amp; Verdone, 2017" authorityName="Kondratieff &amp; Verdone" authorityYear="2017" box="[501,689,960,984]" class="Insecta" family="Perlodidae" genus="Diploperla" kingdom="Animalia" order="Plecoptera" pageId="3" pageNumber="130" phylum="Arthropoda" rank="species" species="janeae" status="sp. n.">
<emphasis box="[501,689,960,984]" italics="true" pageId="3" pageNumber="130">Diploperla janeae</emphasis>
</taxonomicName>
<taxonomicNameLabel box="[695,759,961,984]" pageId="3" pageNumber="130" rank="species">sp. n.</taxonomicNameLabel>
, Helton Creek, Grayson Co., Virginia, 5X.
</paragraph>
</caption>
<subSubSection lastPageId="4" lastPageNumber="131" pageId="3" pageNumber="130" type="description">
<paragraph blockId="3.[189,798,1067,1920]" pageId="3" pageNumber="130">
<emphasis bold="true" box="[189,267,1067,1091]" pageId="3" pageNumber="130">Adult.</emphasis>
Macropterous. Body length, male 
<quantity metricMagnitude="-2" metricUnit="m" metricValue="1.28" metricValueMax="1.39" metricValueMin="1.17" pageId="3" pageNumber="130" unit="mm" value="12.8" valueMax="13.9" valueMin="11.7">11.7–13.9 mm</quantity>
(n = 5); female 
<quantity box="[423,585,1104,1128]" metricMagnitude="-2" metricUnit="m" metricValue="1.27" metricValueMax="1.33" metricValueMin="1.21" pageId="3" pageNumber="130" unit="mm" value="12.7" valueMax="13.3" valueMin="12.1">12.1–13.3 mm</quantity>
(n = 4), Forewing length, male 
<quantity box="[344,506,1140,1164]" metricMagnitude="-2" metricUnit="m" metricValue="1.515" metricValueMax="1.55" metricValueMin="1.48" pageId="3" pageNumber="130" unit="mm" value="15.15" valueMax="15.5" valueMin="14.8">14.8–15.5 mm</quantity>
(n = 5); female 
<quantity metricMagnitude="-2" metricUnit="m" metricValue="1.425" metricValueMax="1.53" metricValueMin="1.3199999999999998" pageId="3" pageNumber="130" unit="mm" value="14.25" valueMax="15.3" valueMin="13.2">13.2–15.3 mm</quantity>
(n= 4). General color yellow to light brown with dark brown to black markings (
<figureCitation box="[612,701,1212,1236]" captionStart="Figs" captionStartId="1.[189,238,1156,1180]" captionTargetBox="[324,1307,254,1073]" captionTargetId="figure-320@1.[324,1307,254,1073]" captionTargetPageId="1" captionText="Figs. 1a–b. Adult habitus of Diploperla janeae sp. n., Helton Creek, Grayson Co., Virginia and D. duplicata, Laurel Fork, Floyd Co., Virginia, 1.5X. Fig. 1a. D. janeae sp. n, habitus, dorsal. Fig. 1b. D. duplicata, habitus, dorsal." figureDoi="http://doi.org/10.5281/zenodo.4757831" httpUri="https://zenodo.org/record/4757831/files/figure.png" pageId="3" pageNumber="130">Fig. 1a</figureCitation>
). Head dorsally with a yellow “spade- shaped” spot that extends from the posterior margin of the head to beyond the epicranial suture, a dark brown to black thick “X- shaped” mark centrally that extends laterally from the anterior ocellus, and a triangular dark brown spot on the frons (
<figureCitation box="[626,729,1428,1452]" captionStart="Figs" captionStartId="1.[189,238,1156,1180]" captionTargetBox="[324,1307,254,1073]" captionTargetId="figure-320@1.[324,1307,254,1073]" captionTargetPageId="1" captionText="Figs. 1a–b. Adult habitus of Diploperla janeae sp. n., Helton Creek, Grayson Co., Virginia and D. duplicata, Laurel Fork, Floyd Co., Virginia, 1.5X. Fig. 1a. D. janeae sp. n, habitus, dorsal. Fig. 1b. D. duplicata, habitus, dorsal." figureDoi="http://doi.org/10.5281/zenodo.4757831" httpUri="https://zenodo.org/record/4757831/files/figure.png" pageId="3" pageNumber="130">Figs. 1a</figureCitation>
, 
<figureCitation box="[755,784,1428,1452]" captionStart="Figs" captionStartId="2.[189,238,865,889]" captionTargetBox="[286,1344,254,783]" captionTargetId="figure-424@2.[286,1344,254,783]" captionTargetPageId="2" captionText="Figs. 2a–b. Adult head and pronotum of Diploperla janeae sp. n., Helton Creek, Grayson Co., Virginia and D. duplicata, Bull Run Mtn, Prince William Co., Virginia, 5X. Fig. 2a. D. janeae sp. n, head and pronotum, dorsal. Fig. 2b. D. duplicata, head and pronotum, dorsal." figureDoi="http://doi.org/10.5281/zenodo.4757833" httpUri="https://zenodo.org/record/4757833/files/figure.png" pageId="3" pageNumber="130">2a</figureCitation>
). Antennae dark brown to black, scape brown, yellowish tinged basally (
<figureCitation box="[509,605,1500,1524]" captionStart="Figs" captionStartId="1.[189,238,1156,1180]" captionTargetBox="[324,1307,254,1073]" captionTargetId="figure-320@1.[324,1307,254,1073]" captionTargetPageId="1" captionText="Figs. 1a–b. Adult habitus of Diploperla janeae sp. n., Helton Creek, Grayson Co., Virginia and D. duplicata, Laurel Fork, Floyd Co., Virginia, 1.5X. Fig. 1a. D. janeae sp. n, habitus, dorsal. Fig. 1b. D. duplicata, habitus, dorsal." figureDoi="http://doi.org/10.5281/zenodo.4757831" httpUri="https://zenodo.org/record/4757831/files/figure.png" pageId="3" pageNumber="130">Figs. 1a</figureCitation>
, 
<figureCitation box="[624,653,1500,1524]" captionStart="Figs" captionStartId="2.[189,238,865,889]" captionTargetBox="[286,1344,254,783]" captionTargetId="figure-424@2.[286,1344,254,783]" captionTargetPageId="2" captionText="Figs. 2a–b. Adult head and pronotum of Diploperla janeae sp. n., Helton Creek, Grayson Co., Virginia and D. duplicata, Bull Run Mtn, Prince William Co., Virginia, 5X. Fig. 2a. D. janeae sp. n, head and pronotum, dorsal. Fig. 2b. D. duplicata, head and pronotum, dorsal." figureDoi="http://doi.org/10.5281/zenodo.4757833" httpUri="https://zenodo.org/record/4757833/files/figure.png" pageId="3" pageNumber="130">2a</figureCitation>
). Prothorax with wide yellow mid-dorsal stripe, slightly expanded anteriorly (
<figureCitation box="[454,549,1572,1596]" captionStart="Figs" captionStartId="1.[189,238,1156,1180]" captionTargetBox="[324,1307,254,1073]" captionTargetId="figure-320@1.[324,1307,254,1073]" captionTargetPageId="1" captionText="Figs. 1a–b. Adult habitus of Diploperla janeae sp. n., Helton Creek, Grayson Co., Virginia and D. duplicata, Laurel Fork, Floyd Co., Virginia, 1.5X. Fig. 1a. D. janeae sp. n, habitus, dorsal. Fig. 1b. D. duplicata, habitus, dorsal." figureDoi="http://doi.org/10.5281/zenodo.4757831" httpUri="https://zenodo.org/record/4757831/files/figure.png" pageId="3" pageNumber="130">Figs. 1a</figureCitation>
, 
<figureCitation box="[567,596,1572,1596]" captionStart="Figs" captionStartId="2.[189,238,865,889]" captionTargetBox="[286,1344,254,783]" captionTargetId="figure-424@2.[286,1344,254,783]" captionTargetPageId="2" captionText="Figs. 2a–b. Adult head and pronotum of Diploperla janeae sp. n., Helton Creek, Grayson Co., Virginia and D. duplicata, Bull Run Mtn, Prince William Co., Virginia, 5X. Fig. 2a. D. janeae sp. n, head and pronotum, dorsal. Fig. 2b. D. duplicata, head and pronotum, dorsal." figureDoi="http://doi.org/10.5281/zenodo.4757833" httpUri="https://zenodo.org/record/4757833/files/figure.png" pageId="3" pageNumber="130">2a</figureCitation>
). Wings slightly fumose, veins dark brown (
<figureCitation box="[533,617,1608,1632]" captionStart="Figs" captionStartId="1.[189,238,1156,1180]" captionTargetBox="[324,1307,254,1073]" captionTargetId="figure-320@1.[324,1307,254,1073]" captionTargetPageId="1" captionText="Figs. 1a–b. Adult habitus of Diploperla janeae sp. n., Helton Creek, Grayson Co., Virginia and D. duplicata, Laurel Fork, Floyd Co., Virginia, 1.5X. Fig. 1a. D. janeae sp. n, habitus, dorsal. Fig. 1b. D. duplicata, habitus, dorsal." figureDoi="http://doi.org/10.5281/zenodo.4757831" httpUri="https://zenodo.org/record/4757831/files/figure.png" pageId="3" pageNumber="130">Fig. 1a</figureCitation>
). Legs yellow, femora banded with dark brown, tibia longitudinally banded with dark brown (
<figureCitation box="[698,782,1680,1704]" captionStart="Figs" captionStartId="1.[189,238,1156,1180]" captionTargetBox="[324,1307,254,1073]" captionTargetId="figure-320@1.[324,1307,254,1073]" captionTargetPageId="1" captionText="Figs. 1a–b. Adult habitus of Diploperla janeae sp. n., Helton Creek, Grayson Co., Virginia and D. duplicata, Laurel Fork, Floyd Co., Virginia, 1.5X. Fig. 1a. D. janeae sp. n, habitus, dorsal. Fig. 1b. D. duplicata, habitus, dorsal." figureDoi="http://doi.org/10.5281/zenodo.4757831" httpUri="https://zenodo.org/record/4757831/files/figure.png" pageId="3" pageNumber="130">Fig. 1a</figureCitation>
). Mesothorax with a pair of large dark brown spots (
<figureCitation box="[197,287,1752,1776]" captionStart="Figs" captionStartId="1.[189,238,1156,1180]" captionTargetBox="[324,1307,254,1073]" captionTargetId="figure-320@1.[324,1307,254,1073]" captionTargetPageId="1" captionText="Figs. 1a–b. Adult habitus of Diploperla janeae sp. n., Helton Creek, Grayson Co., Virginia and D. duplicata, Laurel Fork, Floyd Co., Virginia, 1.5X. Fig. 1a. D. janeae sp. n, habitus, dorsal. Fig. 1b. D. duplicata, habitus, dorsal." figureDoi="http://doi.org/10.5281/zenodo.4757831" httpUri="https://zenodo.org/record/4757831/files/figure.png" pageId="3" pageNumber="130">Fig. 1a</figureCitation>
). Abdomen dorsally shaded with dark brown, yellow ventrally, laterally brown. Cerci dark brown (
<figureCitation box="[281,360,1824,1848]" captionStart="Figs" captionStartId="1.[189,238,1156,1180]" captionTargetBox="[324,1307,254,1073]" captionTargetId="figure-320@1.[324,1307,254,1073]" captionTargetPageId="1" captionText="Figs. 1a–b. Adult habitus of Diploperla janeae sp. n., Helton Creek, Grayson Co., Virginia and D. duplicata, Laurel Fork, Floyd Co., Virginia, 1.5X. Fig. 1a. D. janeae sp. n, habitus, dorsal. Fig. 1b. D. duplicata, habitus, dorsal." figureDoi="http://doi.org/10.5281/zenodo.4757831" httpUri="https://zenodo.org/record/4757831/files/figure.png" pageId="3" pageNumber="130">Fig. 1a</figureCitation>
).
</paragraph>
<paragraph blockId="3.[189,798,1067,1920]" lastBlockId="3.[835,1443,1068,1920]" pageId="3" pageNumber="130">
<emphasis bold="true" box="[189,258,1859,1883]" pageId="3" pageNumber="130">Male.</emphasis>
Seventh and eighth abdominal sterna with well-defined lobes, lobe on seventh narrow, cone-shaped, distal edge darkened; lobe on eighth sternum reduced, broadly rounded (
<figureCitation box="[1280,1350,1104,1128]" captionStart="Fig" captionStartId="3.[189,226,960,984]" captionTargetBox="[504,1128,254,878]" captionTargetId="figure-436@3.[504,1128,254,878]" captionTargetPageId="3" captionText="Fig. 3. Male sterna 6–10 of Diploperla janeae sp. n., Helton Creek, Grayson Co., Virginia, 5X." figureDoi="http://doi.org/10.5281/zenodo.4757837" httpUri="https://zenodo.org/record/4757837/files/figure.png" pageId="3" pageNumber="130">Fig. 3</figureCitation>
). Tenth tergum cleft posteriorly with long hemitergal lobes studded with sensilla basiconica on the anterior surface (
<figureCitation box="[944,1035,1212,1236]" captionStart="Figs" captionStartId="4.[189,238,865,889]" captionTargetBox="[286,1344,254,783]" captionTargetId="figure-504@4.[286,1344,254,783]" captionTargetPageId="4" captionText="Figs. 4a–b. Terminalia of Diploperla janeae sp. n., Helton Creek, Grayson Co., Virginia and D. duplicata, Little Rock Castle Creek, Patrick Co., Virginia, 5X. E. Epiproct, HL. Hemitergal lobe, LS. Lateral stylet. Fig. 4a. D. janeae sp. n, terminalia and lateral stylet, dorsal expansion (arrow), lateral. Fig. 4b. D. duplicata, terminalia, lateral." figureDoi="http://doi.org/10.5281/zenodo.4757839" httpUri="https://zenodo.org/record/4757839/files/figure.png" pageId="3" pageNumber="130">Fig. 4a</figureCitation>
). Lateral stylets irregularly and strongly ribbed throughout, apex expanded dorsally and ventrally, slightly asymmetrical (
<figureCitation captionStart="Figs" captionStartId="4.[189,238,865,889]" captionTargetBox="[286,1344,254,783]" captionTargetId="figure-504@4.[286,1344,254,783]" captionTargetPageId="4" captionText="Figs. 4a–b. Terminalia of Diploperla janeae sp. n., Helton Creek, Grayson Co., Virginia and D. duplicata, Little Rock Castle Creek, Patrick Co., Virginia, 5X. E. Epiproct, HL. Hemitergal lobe, LS. Lateral stylet. Fig. 4a. D. janeae sp. n, terminalia and lateral stylet, dorsal expansion (arrow), lateral. Fig. 4b. D. duplicata, terminalia, lateral." figureDoi="http://doi.org/10.5281/zenodo.4757839" httpUri="https://zenodo.org/record/4757839/files/figure.png" pageId="3" pageNumber="130">Figs. 4a</figureCitation>
, 
<figureCitation box="[878,905,1320,1344]" captionStart="Figs" captionStartId="5.[189,238,1611,1635]" captionTargetBox="[286,1344,999,1528]" captionTargetId="figure-197@5.[286,1344,999,1528]" captionTargetPageId="5" captionText="Figs. 6a–b. Paraprocts of Diploperla janeae sp. n., Helton Creek, Grayson Co., Virginia and D. duplicata, Laurel Fork, Floyd Co., Virginia. Fig. 6a. D. janeae sp. n, paraprocts and dorsal expansion (arrow), lateral, 430X. Fig. 6b. D. duplicata, paraprocts, lateral, 200X." figureDoi="http://doi.org/10.5281/zenodo.4757845" httpUri="https://zenodo.org/record/4757845/files/figure.png" pageId="3" pageNumber="130">6a</figureCitation>
). Epiproct short, membranous, apex bluntly rounded, without setae (
<figureCitation box="[1124,1215,1356,1380]" captionStart="Figs" captionStartId="4.[189,238,865,889]" captionTargetBox="[286,1344,254,783]" captionTargetId="figure-504@4.[286,1344,254,783]" captionTargetPageId="4" captionText="Figs. 4a–b. Terminalia of Diploperla janeae sp. n., Helton Creek, Grayson Co., Virginia and D. duplicata, Little Rock Castle Creek, Patrick Co., Virginia, 5X. E. Epiproct, HL. Hemitergal lobe, LS. Lateral stylet. Fig. 4a. D. janeae sp. n, terminalia and lateral stylet, dorsal expansion (arrow), lateral. Fig. 4b. D. duplicata, terminalia, lateral." figureDoi="http://doi.org/10.5281/zenodo.4757839" httpUri="https://zenodo.org/record/4757839/files/figure.png" pageId="3" pageNumber="130">Figs. 4a</figureCitation>
, 
<figureCitation box="[1227,1255,1356,1380]" captionStart="Figs" captionStartId="5.[189,238,865,889]" captionTargetBox="[286,1344,254,783]" captionTargetId="figure-195@5.[286,1344,254,783]" captionTargetPageId="5" captionText="Figs. 5a–b. Epiproct of Diploperla janeae sp. n., Helton Creek, Grayson Co., Virginia and D. duplicata, Laurel Fork, Floyd Co., Virginia. Fig. 4a. D. janeae sp. n, epiproct, anterior, 1600X. Fig. 5b. D. duplicata, epiproct, lateral, 750X." figureDoi="http://doi.org/10.5281/zenodo.4757843" httpUri="https://zenodo.org/record/4757843/files/figure.png" pageId="3" pageNumber="130">5a</figureCitation>
).
</paragraph>
<paragraph blockId="3.[835,1443,1068,1920]" pageId="3" pageNumber="130">
<emphasis bold="true" box="[835,929,1392,1416]" pageId="3" pageNumber="130">Female.</emphasis>
Subgenital plate parabolic reaching beyond ninth sternum (
<figureCitation box="[1018,1098,1428,1452]" captionStart="Figs" captionStartId="7.[189,238,865,889]" captionTargetBox="[286,1344,254,783]" captionTargetId="figure-548@7.[286,1344,254,783]" captionTargetPageId="7" captionText="Figs. 7a–b. Female subgenital plate of Diploperla janeae sp. n., Helton Creek, Grayson Co., Virginia and D. duplicata, Little Rock Castle Creek, Patrick Co., Virginia, 5X. 7a. D. janeae sp. n, female subgenital plate. 7b. D. duplicata, female subgenital plate." figureDoi="http://doi.org/10.5281/zenodo.4757849" httpUri="https://zenodo.org/record/4757849/files/figure.png" pageId="3" pageNumber="130">Fig. 7a</figureCitation>
).
</paragraph>
<paragraph blockId="3.[835,1443,1068,1920]" box="[835,1051,1463,1488]" pageId="3" pageNumber="130">
<emphasis bold="true" box="[835,918,1463,1487]" pageId="3" pageNumber="130">Ovum.</emphasis>
Unknown.
</paragraph>
<paragraph blockId="3.[835,1443,1068,1920]" lastBlockId="4.[189,798,1081,1932]" lastPageId="4" lastPageNumber="131" pageId="3" pageNumber="130">
<emphasis bold="true" box="[835,910,1500,1524]" pageId="3" pageNumber="130">Larva.</emphasis>
Only larval exuviae of 
<taxonomicName authorityName="Kondratieff &amp; Verdone" authorityYear="2017" box="[1251,1364,1500,1523]" class="Insecta" family="Perlodidae" genus="Diploperla" kingdom="Animalia" order="Plecoptera" pageId="3" pageNumber="130" phylum="Arthropoda" rank="species" species="janeae">
<emphasis box="[1251,1364,1500,1523]" italics="true" pageId="3" pageNumber="130">D. janeae</emphasis>
</taxonomicName>
were available for examination. Only one of these that could be measured had a body length of 
<quantity box="[1312,1412,1572,1596]" metricMagnitude="-2" metricUnit="m" metricValue="1.55" pageId="3" pageNumber="130" unit="mm" value="15.5">15.5 mm</quantity>
(n =1). Dorsum of head with contrasting pattern; frontoclypeus moderately pigmented, brown, concolorous; anterior margin with a dense fringe of fine setae; frons moderately pigmented, brown, with a light colored, well defined M-pattern; a pair of obliquely oriented, small, pale spots lateral to median ocellus. Antennae light brown. Lacinia subtriangular, bidentate with 7 submarginal setae and no marginal setae. Pronotum dark brown; meso- and meta-nota light brown with no distinct markings. Abdomen brown with paired medial pale spots on terga 2–9. Legs lightly pigmented; femora with scattered distally directed stout setae dorsally; tibia and tarsi with a dorsal setal fringe. Cerci brown with circlets of long apical hairs on each segment.
</paragraph>
</subSubSection>
<caption ID-DOI="http://doi.org/10.5281/zenodo.4757839" ID-Zenodo-Dep="4757839" httpUri="https://zenodo.org/record/4757839/files/figure.png" pageId="4" pageNumber="131" startId="4.[189,238,865,889]" targetBox="[286,1344,254,783]" targetPageId="4">
<paragraph blockId="4.[189,1443,865,997]" pageId="4" pageNumber="131">
Figs. 4a–b. Terminalia of 
<taxonomicName authority="Kondratieff &amp; Verdone, 2017" authorityName="Kondratieff &amp; Verdone" authorityYear="2017" box="[483,670,865,889]" class="Insecta" family="Perlodidae" genus="Diploperla" kingdom="Animalia" order="Plecoptera" pageId="4" pageNumber="131" phylum="Arthropoda" rank="species" species="janeae" status="sp. n.">
<emphasis box="[483,670,865,889]" italics="true" pageId="4" pageNumber="131">Diploperla janeae</emphasis>
</taxonomicName>
<taxonomicNameLabel box="[676,738,866,889]" pageId="4" pageNumber="131" rank="species">sp. n.</taxonomicNameLabel>
, Helton Creek, Grayson Co., Virginia and 
<taxonomicName authority=", Little Rock Castle Creek, Patrick Co." authorityName="Little Rock Castle Creek, Patrick Co." class="Insecta" family="Perlodidae" genus="Diploperla" kingdom="Animalia" order="Plecoptera" pageId="4" pageNumber="131" phylum="Arthropoda" rank="species" species="duplicata">
<emphasis box="[1235,1368,865,889]" italics="true" pageId="4" pageNumber="131">D. duplicata</emphasis>
, Little Rock Castle Creek, Patrick Co.
</taxonomicName>
, Virginia, 5X. E. Epiproct, HL. Hemitergal lobe, LS. Lateral stylet. Fig. 4a. 
<taxonomicName authority="Kondratieff &amp; Verdone, 2017" authorityName="Kondratieff &amp; Verdone" authorityYear="2017" class="Insecta" family="Perlodidae" genus="Diploperla" kingdom="Animalia" order="Plecoptera" pageId="4" pageNumber="131" phylum="Arthropoda" rank="species" species="janeae" status="sp. n">
<emphasis italics="true" pageId="4" pageNumber="131">D. janeae</emphasis>
</taxonomicName>
<taxonomicNameLabel box="[263,321,938,961]" pageId="4" pageNumber="131" rank="species">sp. n</taxonomicNameLabel>
, terminalia and lateral stylet, dorsal expansion (arrow), lateral. Fig. 4b. 
<taxonomicName box="[1168,1302,937,961]" class="Insecta" family="Perlodidae" genus="Diploperla" kingdom="Animalia" order="Plecoptera" pageId="4" pageNumber="131" phylum="Arthropoda" rank="species" species="duplicata">
<emphasis box="[1168,1302,937,961]" italics="true" pageId="4" pageNumber="131">D. duplicata</emphasis>
</taxonomicName>
, terminalia, lateral.
</paragraph>
</caption>
<subSubSection box="[189,627,1296,1321]" pageId="4" pageNumber="131" type="distribution">
<paragraph blockId="4.[189,798,1081,1932]" box="[189,627,1296,1321]" pageId="4" pageNumber="131">
<emphasis bold="true" box="[189,348,1296,1320]" pageId="4" pageNumber="131">Distribution.</emphasis>
<collectingCountry box="[355,411,1297,1321]" name="United States of America" pageId="4" pageNumber="131">USA</collectingCountry>
– NC, VA (
<figureCitation box="[546,612,1297,1321]" captionStart="Fig" captionStartId="11.[189,226,1633,1657]" captionTargetBox="[286,1344,218,1587]" captionTargetId="figure-130@11.[286,1344,218,1587]" captionTargetPageId="11" captionText="Fig. 9. Distribution map of examined material and physiographic provinces. See text for comprehensive list of states occupied for some species." figureDoi="http://doi.org/10.5281/zenodo.4757855" httpUri="https://zenodo.org/record/4757855/files/figure.png" pageId="4" pageNumber="131">Fig. 9</figureCitation>
).
</paragraph>
</subSubSection>
<subSubSection pageId="4" pageNumber="131" type="etymology">
<paragraph blockId="4.[189,798,1081,1932]" pageId="4" pageNumber="131">
<emphasis bold="true" box="[189,327,1333,1357]" pageId="4" pageNumber="131">Etymology.</emphasis>
The new species is named for Jane Earle, Mechanicsburg, 
<collectingRegion box="[455,616,1369,1393]" country="United States of America" name="Pennsylvania" pageId="4" pageNumber="131">Pennsylvania</collectingRegion>
, in honor of her contributions to the study of North American 
<taxonomicName box="[189,317,1441,1465]" class="Insecta" kingdom="Animalia" order="Plecoptera" pageId="4" pageNumber="131" phylum="Arthropoda" rank="order">Plecoptera</taxonomicName>
. Jane has also been a loyal participant of the International Society of Plecopterologists. We propose the following common name of “Jane’s Stripetail” for this species.
</paragraph>
</subSubSection>
<subSubSection lastPageId="5" lastPageNumber="132" pageId="4" pageNumber="131" type="diagnosis">
<paragraph blockId="4.[189,798,1081,1932]" lastBlockId="5.[835,1444,1791,1887]" lastPageId="5" lastPageNumber="132" pageId="4" pageNumber="131">
<emphasis bold="true" box="[189,319,1584,1608]" pageId="4" pageNumber="131">Diagnosis.</emphasis>
<taxonomicName authorityName="Kondratieff &amp; Verdone" authorityYear="2017" box="[331,523,1584,1608]" class="Insecta" family="Perlodidae" genus="Diploperla" kingdom="Animalia" order="Plecoptera" pageId="4" pageNumber="131" phylum="Arthropoda" rank="species" species="janeae">
<emphasis box="[331,523,1584,1608]" italics="true" pageId="4" pageNumber="131">Diploperla janeae</emphasis>
</taxonomicName>
is distinguished from the most similar species, 
<taxonomicName box="[504,644,1621,1645]" class="Insecta" family="Perlodidae" genus="Diploperla" kingdom="Animalia" order="Plecoptera" pageId="4" pageNumber="131" phylum="Arthropoda" rank="species" species="duplicata">
<emphasis box="[504,644,1621,1645]" italics="true" pageId="4" pageNumber="131">D. duplicata</emphasis>
</taxonomicName>
, in genitalic structures, its dark coloration, and conspicuous brown to black dorsal head markings. Both species possess lateral stylets with the apices expanded. However, the apices of the new species are expanded dorsally and ventrally (
<figureCitation box="[631,732,1801,1825]" captionStart="Figs" captionStartId="4.[189,238,865,889]" captionTargetBox="[286,1344,254,783]" captionTargetId="figure-504@4.[286,1344,254,783]" captionTargetPageId="4" captionText="Figs. 4a–b. Terminalia of Diploperla janeae sp. n., Helton Creek, Grayson Co., Virginia and D. duplicata, Little Rock Castle Creek, Patrick Co., Virginia, 5X. E. Epiproct, HL. Hemitergal lobe, LS. Lateral stylet. Fig. 4a. D. janeae sp. n, terminalia and lateral stylet, dorsal expansion (arrow), lateral. Fig. 4b. D. duplicata, terminalia, lateral." figureDoi="http://doi.org/10.5281/zenodo.4757839" httpUri="https://zenodo.org/record/4757839/files/figure.png" pageId="4" pageNumber="131">Figs. 4a</figureCitation>
, 
<figureCitation box="[754,783,1801,1825]" captionStart="Figs" captionStartId="5.[189,238,1611,1635]" captionTargetBox="[286,1344,999,1528]" captionTargetId="figure-197@5.[286,1344,999,1528]" captionTargetPageId="5" captionText="Figs. 6a–b. Paraprocts of Diploperla janeae sp. n., Helton Creek, Grayson Co., Virginia and D. duplicata, Laurel Fork, Floyd Co., Virginia. Fig. 6a. D. janeae sp. n, paraprocts and dorsal expansion (arrow), lateral, 430X. Fig. 6b. D. duplicata, paraprocts, lateral, 200X." figureDoi="http://doi.org/10.5281/zenodo.4757845" httpUri="https://zenodo.org/record/4757845/files/figure.png" pageId="4" pageNumber="131">6a</figureCitation>
), whereas in 
<taxonomicName box="[322,455,1836,1860]" class="Insecta" family="Perlodidae" genus="Diploperla" kingdom="Animalia" order="Plecoptera" pageId="4" pageNumber="131" phylum="Arthropoda" rank="species" species="duplicata">
<emphasis box="[322,455,1836,1860]" italics="true" pageId="4" pageNumber="131">D. duplicata</emphasis>
</taxonomicName>
the apices are expanded only ventrally (
<figureCitation box="[323,428,1873,1897]" captionStart="Figs" captionStartId="4.[189,238,865,889]" captionTargetBox="[286,1344,254,783]" captionTargetId="figure-504@4.[286,1344,254,783]" captionTargetPageId="4" captionText="Figs. 4a–b. Terminalia of Diploperla janeae sp. n., Helton Creek, Grayson Co., Virginia and D. duplicata, Little Rock Castle Creek, Patrick Co., Virginia, 5X. E. Epiproct, HL. Hemitergal lobe, LS. Lateral stylet. Fig. 4a. D. janeae sp. n, terminalia and lateral stylet, dorsal expansion (arrow), lateral. Fig. 4b. D. duplicata, terminalia, lateral." figureDoi="http://doi.org/10.5281/zenodo.4757839" httpUri="https://zenodo.org/record/4757839/files/figure.png" pageId="4" pageNumber="131">Figs. 4b</figureCitation>
, 
<figureCitation box="[453,483,1873,1897]" captionStart="Figs" captionStartId="5.[189,238,1611,1635]" captionTargetBox="[286,1344,999,1528]" captionTargetId="figure-197@5.[286,1344,999,1528]" captionTargetPageId="5" captionText="Figs. 6a–b. Paraprocts of Diploperla janeae sp. n., Helton Creek, Grayson Co., Virginia and D. duplicata, Laurel Fork, Floyd Co., Virginia. Fig. 6a. D. janeae sp. n, paraprocts and dorsal expansion (arrow), lateral, 430X. Fig. 6b. D. duplicata, paraprocts, lateral, 200X." figureDoi="http://doi.org/10.5281/zenodo.4757845" httpUri="https://zenodo.org/record/4757845/files/figure.png" pageId="4" pageNumber="131">6b</figureCitation>
). Both species are also distinguished by the apex of the epiproct. In 
<taxonomicName authorityName="Kondratieff &amp; Verdone" authorityYear="2017" box="[770,901,1082,1932]" class="Insecta" family="Perlodidae" genus="Diploperla" kingdom="Animalia" order="Plecoptera" pageId="4" pageNumber="131" phylum="Arthropoda" rank="species" species="janeae">
<emphasis box="[770,901,1082,1932]" italics="true" pageId="4" pageNumber="131">D. janeae</emphasis>
</taxonomicName>
, the epiproct apex is bluntly rounded without setae (
<figureCitation box="[909,999,1117,1141]" captionStart="Figs" captionStartId="4.[189,238,865,889]" captionTargetBox="[286,1344,254,783]" captionTargetId="figure-504@4.[286,1344,254,783]" captionTargetPageId="4" captionText="Figs. 4a–b. Terminalia of Diploperla janeae sp. n., Helton Creek, Grayson Co., Virginia and D. duplicata, Little Rock Castle Creek, Patrick Co., Virginia, 5X. E. Epiproct, HL. Hemitergal lobe, LS. Lateral stylet. Fig. 4a. D. janeae sp. n, terminalia and lateral stylet, dorsal expansion (arrow), lateral. Fig. 4b. D. duplicata, terminalia, lateral." figureDoi="http://doi.org/10.5281/zenodo.4757839" httpUri="https://zenodo.org/record/4757839/files/figure.png" pageId="4" pageNumber="131">Figs. 4a</figureCitation>
, 
<figureCitation box="[1011,1039,1117,1141]" captionStart="Figs" captionStartId="5.[189,238,865,889]" captionTargetBox="[286,1344,254,783]" captionTargetId="figure-195@5.[286,1344,254,783]" captionTargetPageId="5" captionText="Figs. 5a–b. Epiproct of Diploperla janeae sp. n., Helton Creek, Grayson Co., Virginia and D. duplicata, Laurel Fork, Floyd Co., Virginia. Fig. 4a. D. janeae sp. n, epiproct, anterior, 1600X. Fig. 5b. D. duplicata, epiproct, lateral, 750X." figureDoi="http://doi.org/10.5281/zenodo.4757843" httpUri="https://zenodo.org/record/4757843/files/figure.png" pageId="4" pageNumber="131">5a</figureCitation>
), whereas the epiproct apex of the 
<taxonomicName box="[835,968,1153,1177]" class="Insecta" family="Perlodidae" genus="Diploperla" kingdom="Animalia" order="Plecoptera" pageId="4" pageNumber="131" phylum="Arthropoda" rank="species" species="duplicata">
<emphasis box="[835,968,1153,1177]" italics="true" pageId="4" pageNumber="131">D. duplicata</emphasis>
</taxonomicName>
is more elongate with sparse setae (
<figureCitation captionStart="Figs" captionStartId="4.[189,238,865,889]" captionTargetBox="[286,1344,254,783]" captionTargetId="figure-504@4.[286,1344,254,783]" captionTargetPageId="4" captionText="Figs. 4a–b. Terminalia of Diploperla janeae sp. n., Helton Creek, Grayson Co., Virginia and D. duplicata, Little Rock Castle Creek, Patrick Co., Virginia, 5X. E. Epiproct, HL. Hemitergal lobe, LS. Lateral stylet. Fig. 4a. D. janeae sp. n, terminalia and lateral stylet, dorsal expansion (arrow), lateral. Fig. 4b. D. duplicata, terminalia, lateral." figureDoi="http://doi.org/10.5281/zenodo.4757839" httpUri="https://zenodo.org/record/4757839/files/figure.png" pageId="4" pageNumber="131">Figs. 4b</figureCitation>
, 
<figureCitation box="[879,910,1189,1213]" captionStart="Figs" captionStartId="5.[189,238,865,889]" captionTargetBox="[286,1344,254,783]" captionTargetId="figure-195@5.[286,1344,254,783]" captionTargetPageId="5" captionText="Figs. 5a–b. Epiproct of Diploperla janeae sp. n., Helton Creek, Grayson Co., Virginia and D. duplicata, Laurel Fork, Floyd Co., Virginia. Fig. 4a. D. janeae sp. n, epiproct, anterior, 1600X. Fig. 5b. D. duplicata, epiproct, lateral, 750X." figureDoi="http://doi.org/10.5281/zenodo.4757843" httpUri="https://zenodo.org/record/4757843/files/figure.png" pageId="4" pageNumber="131">5b</figureCitation>
). The female subgenital plate of 
<taxonomicName authorityName="Kondratieff &amp; Verdone" authorityYear="2017" box="[1311,1414,1190,1213]" class="Insecta" family="Perlodidae" genus="Diploperla" kingdom="Animalia" order="Plecoptera" pageId="4" pageNumber="131" phylum="Arthropoda" rank="species" species="janeae">
<emphasis box="[1311,1414,1190,1213]" italics="true" pageId="4" pageNumber="131">D. janeae</emphasis>
</taxonomicName>
is similar to 
<taxonomicName box="[980,1127,1225,1249]" class="Insecta" family="Perlodidae" genus="Diploperla" kingdom="Animalia" order="Plecoptera" pageId="4" pageNumber="131" phylum="Arthropoda" rank="species" species="duplicata">
<emphasis box="[980,1127,1225,1249]" italics="true" pageId="4" pageNumber="131">D. duplicata</emphasis>
</taxonomicName>
(
<figureCitation box="[1155,1314,1225,1249]" captionStart="Figs" captionStartId="7.[189,238,865,889]" captionTargetBox="[286,1344,254,783]" captionTargetId="figure-548@7.[286,1344,254,783]" captionTargetPageId="7" captionText="Figs. 7a–b. Female subgenital plate of Diploperla janeae sp. n., Helton Creek, Grayson Co., Virginia and D. duplicata, Little Rock Castle Creek, Patrick Co., Virginia, 5X. 7a. D. janeae sp. n, female subgenital plate. 7b. D. duplicata, female subgenital plate." figureDoi="http://doi.org/10.5281/zenodo.4757849" httpUri="https://zenodo.org/record/4757849/files/figure.png" pageId="4" pageNumber="131">Figs. 7a, 7b</figureCitation>
), but the distinctive habitus coloration will serve to distinguish both taxa. 
<taxonomicName authorityName="Kondratieff &amp; Verdone" authorityYear="2017" box="[1104,1294,1297,1321]" class="Insecta" family="Perlodidae" genus="Diploperla" kingdom="Animalia" order="Plecoptera" pageId="4" pageNumber="131" phylum="Arthropoda" rank="species" species="janeae">
<emphasis box="[1104,1294,1297,1321]" italics="true" pageId="4" pageNumber="131">Diploperla janeae</emphasis>
</taxonomicName>
is yellow to light brown with dark brown to black markings, slightly fumose wings with dark brown veins, and dark brown antennae and cerci (
<figureCitation box="[1222,1301,1405,1429]" captionStart="Figs" captionStartId="1.[189,238,1156,1180]" captionTargetBox="[324,1307,254,1073]" captionTargetId="figure-320@1.[324,1307,254,1073]" captionTargetPageId="1" captionText="Figs. 1a–b. Adult habitus of Diploperla janeae sp. n., Helton Creek, Grayson Co., Virginia and D. duplicata, Laurel Fork, Floyd Co., Virginia, 1.5X. Fig. 1a. D. janeae sp. n, habitus, dorsal. Fig. 1b. D. duplicata, habitus, dorsal." figureDoi="http://doi.org/10.5281/zenodo.4757831" httpUri="https://zenodo.org/record/4757831/files/figure.png" pageId="4" pageNumber="131">Fig. 1a</figureCitation>
), compared to 
<taxonomicName box="[867,1002,1441,1465]" class="Insecta" family="Perlodidae" genus="Diploperla" kingdom="Animalia" order="Plecoptera" pageId="4" pageNumber="131" phylum="Arthropoda" rank="species" species="duplicata">
<emphasis box="[867,1002,1441,1465]" italics="true" pageId="4" pageNumber="131">D. duplicata</emphasis>
</taxonomicName>
which is yellow with light brown to brown markings, amber colored wings with light brown veins to light brown to brown antennae and cerci (
<figureCitation box="[905,986,1549,1573]" captionStart="Figs" captionStartId="1.[189,238,1156,1180]" captionTargetBox="[324,1307,254,1073]" captionTargetId="figure-320@1.[324,1307,254,1073]" captionTargetPageId="1" captionText="Figs. 1a–b. Adult habitus of Diploperla janeae sp. n., Helton Creek, Grayson Co., Virginia and D. duplicata, Laurel Fork, Floyd Co., Virginia, 1.5X. Fig. 1a. D. janeae sp. n, habitus, dorsal. Fig. 1b. D. duplicata, habitus, dorsal." figureDoi="http://doi.org/10.5281/zenodo.4757831" httpUri="https://zenodo.org/record/4757831/files/figure.png" pageId="4" pageNumber="131">Fig. 1b</figureCitation>
). Additionally, the head pattern of the two species differ. The head of 
<taxonomicName authorityName="Kondratieff &amp; Verdone" authorityYear="2017" box="[1247,1354,1585,1608]" class="Insecta" family="Perlodidae" genus="Diploperla" kingdom="Animalia" order="Plecoptera" pageId="4" pageNumber="131" phylum="Arthropoda" rank="species" species="janeae">
<emphasis box="[1247,1354,1585,1608]" italics="true" pageId="4" pageNumber="131">D. janeae</emphasis>
</taxonomicName>
, has a yellow “spade- shaped” spot that extends from the posterior margin of the head to beyond the epicranial suture, and a dark brown to black thick “X- shaped” mark centrally that extends laterally from the anterior ocellus (
<figureCitation box="[1168,1256,1765,1789]" captionStart="Figs" captionStartId="2.[189,238,865,889]" captionTargetBox="[286,1344,254,783]" captionTargetId="figure-424@2.[286,1344,254,783]" captionTargetPageId="2" captionText="Figs. 2a–b. Adult head and pronotum of Diploperla janeae sp. n., Helton Creek, Grayson Co., Virginia and D. duplicata, Bull Run Mtn, Prince William Co., Virginia, 5X. Fig. 2a. D. janeae sp. n, head and pronotum, dorsal. Fig. 2b. D. duplicata, head and pronotum, dorsal." figureDoi="http://doi.org/10.5281/zenodo.4757833" httpUri="https://zenodo.org/record/4757833/files/figure.png" pageId="4" pageNumber="131">Fig. 2a</figureCitation>
). Whereas, the head of 
<taxonomicName box="[928,1061,1801,1825]" class="Insecta" family="Perlodidae" genus="Diploperla" kingdom="Animalia" order="Plecoptera" pageId="4" pageNumber="131" phylum="Arthropoda" rank="species" species="duplicata">
<emphasis box="[928,1061,1801,1825]" italics="true" pageId="4" pageNumber="131">D. duplicata</emphasis>
</taxonomicName>
usually has a closed light brown spot covering the interocellular area with arms that extend anteriorly from the anterior ocellus (
<figureCitation box="[1348,1431,1873,1897]" captionStart="Figs" captionStartId="2.[189,238,865,889]" captionTargetBox="[286,1344,254,783]" captionTargetId="figure-424@2.[286,1344,254,783]" captionTargetPageId="2" captionText="Figs. 2a–b. Adult head and pronotum of Diploperla janeae sp. n., Helton Creek, Grayson Co., Virginia and D. duplicata, Bull Run Mtn, Prince William Co., Virginia, 5X. Fig. 2a. D. janeae sp. n, head and pronotum, dorsal. Fig. 2b. D. duplicata, head and pronotum, dorsal." figureDoi="http://doi.org/10.5281/zenodo.4757833" httpUri="https://zenodo.org/record/4757833/files/figure.png" pageId="4" pageNumber="131">Fig. 2b</figureCitation>
). Three other 
<taxonomicName box="[978,1185,1908,1932]" class="Insecta" family="Perlodidae" genus="Diploperla" kingdom="Animalia" order="Plecoptera" pageId="4" pageNumber="131" phylum="Arthropoda" rank="species" species="undetermined">
<emphasis box="[978,1092,1908,1932]" italics="true" pageId="4" pageNumber="131">Diploperla</emphasis>
species
</taxonomicName>
, 
<emphasis italics="true" lastPageId="5" lastPageNumber="132" pageId="4" pageNumber="131">
<taxonomicName authorityName="Kirchner &amp; Kondratieff" authorityYear="1984" box="[1196,1389,1908,1932]" class="Insecta" family="Perlodidae" genus="Diploperla" kingdom="Animalia" order="Plecoptera" pageId="4" pageNumber="131" phylum="Arthropoda" rank="species" species="kanawholensis">D. kanawholensis</taxonomicName>
, 
<taxonomicName authorityName="Kondratieff &amp; Voshell" authorityYear="1979" box="[189,315,1791,1814]" class="Insecta" family="Perlodidae" genus="Diploperla" kingdom="Animalia" order="Plecoptera" pageId="5" pageNumber="132" phylum="Arthropoda" rank="species" species="morgani">D. morgani</taxonomicName>
</emphasis>
, and 
<emphasis box="[383,505,1791,1815]" italics="true" pageId="5" pageNumber="132">
<taxonomicName authorityName="Stark &amp; Gaufin" authorityYear="1974" box="[383,501,1791,1815]" class="Insecta" family="Perlodidae" genus="Diploperla" kingdom="Animalia" order="Plecoptera" pageId="5" pageNumber="132" phylum="Arthropoda" rank="species" species="robusta">D. robusta</taxonomicName>
,
</emphasis>
are darkly marked as 
<taxonomicName authorityName="Kondratieff &amp; Verdone" authorityYear="2017" class="Insecta" family="Perlodidae" genus="Diploperla" kingdom="Animalia" order="Plecoptera" pageId="5" pageNumber="132" phylum="Arthropoda" rank="species" species="janeae">
<emphasis italics="true" pageId="5" pageNumber="132">D. janeae</emphasis>
</taxonomicName>
. But males are easily distinguished by the genitalic characters and the shape of the lobe of sternum 7, which is broadly rounded apically (fig. 8.90, 
<bibRefCitation author="Kondratieff, B. C." box="[892,1082,1791,1815]" pageId="5" pageNumber="132" pagination="1 - 192" refId="ref8569" refString="Kondratieff, B. C. 2004. Perlodidae - Perlodinae (The Springflies). Pp. 149 - 180. In: B. P. Stark &amp; B. J. Armitage (eds.). The stoneflies (Plecoptera) of eastern North America. Volume II. Chloroperlidae, Perlidae, and Perlodidae). Ohio Biological Survey Bulletin New Series Volume 14 (4): 1 - 192." type="journal article" year="2004">Kondratieff 2004</bibRefCitation>
) in 
<emphasis box="[1123,1437,1791,1815]" italics="true" pageId="5" pageNumber="132">
<taxonomicName authorityName="Kirchner &amp; Kondratieff" authorityYear="1984" box="[1123,1307,1791,1815]" class="Insecta" family="Perlodidae" genus="Diploperla" kingdom="Animalia" order="Plecoptera" pageId="5" pageNumber="132" phylum="Arthropoda" rank="species" species="kanawholensis">D. kanawholensis</taxonomicName>
, 
<taxonomicName authorityName="Kondratieff &amp; Voshell" authorityYear="1979" box="[1317,1437,1791,1814]" class="Insecta" family="Perlodidae" genus="Diploperla" kingdom="Animalia" order="Plecoptera" pageId="5" pageNumber="132" phylum="Arthropoda" rank="species" species="morgani">D. morgani</taxonomicName>
</emphasis>
, and 
<taxonomicName authorityName="Stark &amp; Gaufin" authorityYear="1974" box="[886,997,1827,1851]" class="Insecta" family="Perlodidae" genus="Diploperla" kingdom="Animalia" order="Plecoptera" pageId="5" pageNumber="132" phylum="Arthropoda" rank="species" species="robusta">
<emphasis box="[886,997,1827,1851]" italics="true" pageId="5" pageNumber="132">D. robusta</emphasis>
</taxonomicName>
, and narrowly rounded apically in the new species (
<figureCitation box="[984,1046,1863,1887]" captionStart="Fig" captionStartId="3.[189,226,960,984]" captionTargetBox="[504,1128,254,878]" captionTargetId="figure-436@3.[504,1128,254,878]" captionTargetPageId="3" captionText="Fig. 3. Male sterna 6–10 of Diploperla janeae sp. n., Helton Creek, Grayson Co., Virginia, 5X." figureDoi="http://doi.org/10.5281/zenodo.4757837" httpUri="https://zenodo.org/record/4757837/files/figure.png" pageId="5" pageNumber="132">Fig. 3</figureCitation>
).
</paragraph>
</subSubSection>
<caption ID-DOI="http://doi.org/10.5281/zenodo.4757843" ID-Zenodo-Dep="4757843" httpUri="https://zenodo.org/record/4757843/files/figure.png" pageId="5" pageNumber="132" startId="5.[189,238,865,889]" targetBox="[286,1344,254,783]" targetPageId="5">
<paragraph blockId="5.[189,1443,865,961]" pageId="5" pageNumber="132">
Figs. 5a–b. Epiproct of 
<taxonomicName authority="Kondratieff &amp; Verdone, 2017" authorityName="Kondratieff &amp; Verdone" authorityYear="2017" box="[459,646,865,889]" class="Insecta" family="Perlodidae" genus="Diploperla" kingdom="Animalia" order="Plecoptera" pageId="5" pageNumber="132" phylum="Arthropoda" rank="species" species="janeae" status="sp. n.">
<emphasis box="[459,646,865,889]" italics="true" pageId="5" pageNumber="132">Diploperla janeae</emphasis>
</taxonomicName>
<taxonomicNameLabel box="[653,717,866,889]" pageId="5" pageNumber="132" rank="species">sp. n.</taxonomicNameLabel>
, Helton Creek, Grayson Co., Virginia and 
<taxonomicName authority=", Laurel Fork, Floyd Co." authorityName="Laurel Fork, Floyd Co." class="Insecta" family="Perlodidae" genus="Diploperla" kingdom="Animalia" order="Plecoptera" pageId="5" pageNumber="132" phylum="Arthropoda" rank="species" species="duplicata">
<emphasis box="[1218,1353,865,889]" italics="true" pageId="5" pageNumber="132">D. duplicata</emphasis>
, Laurel Fork, Floyd Co.
</taxonomicName>
, Virginia. Fig. 4a. 
<taxonomicName authority="Kondratieff &amp; Verdone, 2017" authorityName="Kondratieff &amp; Verdone" authorityYear="2017" box="[598,700,902,925]" class="Insecta" family="Perlodidae" genus="Diploperla" kingdom="Animalia" order="Plecoptera" pageId="5" pageNumber="132" phylum="Arthropoda" rank="species" species="janeae" status="sp. n">
<emphasis box="[598,700,902,925]" italics="true" pageId="5" pageNumber="132">D. janeae</emphasis>
</taxonomicName>
<taxonomicNameLabel box="[709,769,902,925]" pageId="5" pageNumber="132" rank="species">sp. n</taxonomicNameLabel>
, epiproct, anterior, 1600X. Fig. 5b. 
<taxonomicName box="[1187,1323,902,926]" class="Insecta" family="Perlodidae" genus="Diploperla" kingdom="Animalia" order="Plecoptera" pageId="5" pageNumber="132" phylum="Arthropoda" rank="species" species="duplicata">
<emphasis box="[1187,1323,902,926]" italics="true" pageId="5" pageNumber="132">D. duplicata</emphasis>
</taxonomicName>
, epiproct, lateral, 750X.
</paragraph>
</caption>
<caption ID-DOI="http://doi.org/10.5281/zenodo.4757845" ID-Zenodo-Dep="4757845" httpUri="https://zenodo.org/record/4757845/files/figure.png" pageId="5" pageNumber="132" startId="5.[189,238,1611,1635]" targetBox="[286,1344,999,1528]" targetPageId="5">
<paragraph blockId="5.[189,1443,1611,1707]" pageId="5" pageNumber="132">
Figs. 6a–b. Paraprocts of 
<taxonomicName authority="Kondratieff &amp; Verdone, 2017" authorityName="Kondratieff &amp; Verdone" authorityYear="2017" box="[477,663,1611,1635]" class="Insecta" family="Perlodidae" genus="Diploperla" kingdom="Animalia" order="Plecoptera" pageId="5" pageNumber="132" phylum="Arthropoda" rank="species" species="janeae" status="sp. n.">
<emphasis box="[477,663,1611,1635]" italics="true" pageId="5" pageNumber="132">Diploperla janeae</emphasis>
</taxonomicName>
<taxonomicNameLabel box="[669,731,1611,1634]" pageId="5" pageNumber="132" rank="species">sp. n.</taxonomicNameLabel>
, Helton Creek, Grayson Co., Virginia and 
<taxonomicName authority=", Laurel Fork, Floyd Co." authorityName="Laurel Fork, Floyd Co." class="Insecta" family="Perlodidae" genus="Diploperla" kingdom="Animalia" order="Plecoptera" pageId="5" pageNumber="132" phylum="Arthropoda" rank="species" species="duplicata">
<emphasis box="[1222,1355,1611,1635]" italics="true" pageId="5" pageNumber="132">D. duplicata</emphasis>
, Laurel Fork, Floyd Co.
</taxonomicName>
, Virginia. Fig. 6a. 
<taxonomicName authority="Kondratieff &amp; Verdone, 2017" authorityName="Kondratieff &amp; Verdone" authorityYear="2017" box="[599,702,1648,1671]" class="Insecta" family="Perlodidae" genus="Diploperla" kingdom="Animalia" order="Plecoptera" pageId="5" pageNumber="132" phylum="Arthropoda" rank="species" species="janeae" status="sp. n">
<emphasis box="[599,702,1648,1671]" italics="true" pageId="5" pageNumber="132">D. janeae</emphasis>
</taxonomicName>
<taxonomicNameLabel box="[710,768,1648,1671]" pageId="5" pageNumber="132" rank="species">sp. n</taxonomicNameLabel>
, paraprocts and dorsal expansion (arrow), lateral, 430X. Fig. 6b. 
<taxonomicName box="[281,415,1683,1707]" class="Insecta" family="Perlodidae" genus="Diploperla" kingdom="Animalia" order="Plecoptera" pageId="5" pageNumber="132" phylum="Arthropoda" rank="species" species="duplicata">
<emphasis box="[281,415,1683,1707]" italics="true" pageId="5" pageNumber="132">D. duplicata</emphasis>
</taxonomicName>
, paraprocts, lateral, 200X.
</paragraph>
</caption>
<caption ID-DOI="http://doi.org/10.5281/zenodo.4757849" ID-Zenodo-Dep="4757849" httpUri="https://zenodo.org/record/4757849/files/figure.png" pageId="7" pageNumber="134" startId="7.[189,238,865,889]" targetBox="[286,1344,254,783]" targetPageId="7">
<paragraph blockId="7.[189,1443,865,961]" pageId="7" pageNumber="134">
Figs. 7a–b. Female subgenital plate of 
<taxonomicName authority="Kondratieff &amp; Verdone, 2017" authorityName="Kondratieff &amp; Verdone" authorityYear="2017" box="[644,833,865,889]" class="Insecta" family="Perlodidae" genus="Diploperla" kingdom="Animalia" order="Plecoptera" pageId="7" pageNumber="134" phylum="Arthropoda" rank="species" species="janeae" status="sp. n.">
<emphasis box="[644,833,865,889]" italics="true" pageId="7" pageNumber="134">Diploperla janeae</emphasis>
</taxonomicName>
<taxonomicNameLabel box="[841,906,866,889]" pageId="7" pageNumber="134" rank="species">sp. n.</taxonomicNameLabel>
, Helton Creek, Grayson Co., Virginia and 
<taxonomicName authority=", Little Rock Castle Creek, Patrick Co." authorityName="Little Rock Castle Creek, Patrick Co." class="Insecta" family="Perlodidae" genus="Diploperla" kingdom="Animalia" order="Plecoptera" pageId="7" pageNumber="134" phylum="Arthropoda" rank="species" species="duplicata">
<emphasis italics="true" pageId="7" pageNumber="134">D. duplicata</emphasis>
, Little Rock Castle Creek, Patrick Co.
</taxonomicName>
, Virginia, 5X. 7a. 
<taxonomicName authority="Kondratieff &amp; Verdone, 2017" authorityName="Kondratieff &amp; Verdone" authorityYear="2017" box="[941,1042,902,925]" class="Insecta" family="Perlodidae" genus="Diploperla" kingdom="Animalia" order="Plecoptera" pageId="7" pageNumber="134" phylum="Arthropoda" rank="species" species="janeae" status="sp. n">
<emphasis box="[941,1042,902,925]" italics="true" pageId="7" pageNumber="134">D. janeae</emphasis>
</taxonomicName>
<taxonomicNameLabel box="[1049,1108,902,925]" pageId="7" pageNumber="134" rank="species">sp. n</taxonomicNameLabel>
, female subgenital plate. 7b. 
<taxonomicName box="[189,323,937,961]" class="Insecta" family="Perlodidae" genus="Diploperla" kingdom="Animalia" order="Plecoptera" pageId="7" pageNumber="134" phylum="Arthropoda" rank="species" species="duplicata">
<emphasis box="[189,323,937,961]" italics="true" pageId="7" pageNumber="134">D. duplicata</emphasis>
</taxonomicName>
, female subgenital plate.
</paragraph>
</caption>
<caption ID-DOI="http://doi.org/10.5281/zenodo.4757853" ID-Zenodo-Dep="4757853" box="[189,937,1094,1118]" httpUri="https://zenodo.org/record/4757853/files/figure.png" pageId="9" pageNumber="136" startId="9.[189,226,1094,1118]" targetBox="[286,1344,218,1012]" targetPageId="9">
<paragraph blockId="9.[189,937,1094,1118]" box="[189,937,1094,1118]" pageId="9" pageNumber="136">Fig. 8. Type locality. Helton Creek, Grayson Co., Virginia, USA.</paragraph>
</caption>
<subSubSection lastPageId="12" lastPageNumber="139" pageId="10" pageNumber="137" type="biology_ecology">
<paragraph blockId="10.[835,1444,228,1836]" lastBlockId="12.[189,798,228,1079]" lastPageId="12" lastPageNumber="139" pageId="10" pageNumber="137">
<emphasis bold="true" box="[835,1037,1559,1583]" pageId="10" pageNumber="137">Biological notes.</emphasis>
There is no information about the biology or life cycle of 
<taxonomicName authorityName="Kondratieff &amp; Verdone" authorityYear="2017" box="[1145,1253,1596,1619]" class="Insecta" family="Perlodidae" genus="Diploperla" kingdom="Animalia" order="Plecoptera" pageId="10" pageNumber="137" phylum="Arthropoda" rank="species" species="janeae">
<emphasis box="[1145,1253,1596,1619]" italics="true" pageId="10" pageNumber="137">D. janeae</emphasis>
</taxonomicName>
. Based on the available records, the emergence period appears to be from early to late May. The new species has been collected from 1 
<superScript attach="left" box="[1033,1045,1704,1718]" fontSize="6" pageId="10" pageNumber="137">st</superScript>
to 4 
<superScript attach="left" box="[1104,1119,1704,1718]" fontSize="6" pageId="10" pageNumber="137">th</superScript>
order streams in the Blue Ridge, and Ridge and Valley physiographic provinces from southwestern 
<collectingRegion box="[1284,1381,1775,1799]" country="United States of America" name="Virginia" pageId="10" pageNumber="137">Virginia</collectingRegion>
to southwestern 
<collectingRegion box="[1001,1184,1811,1835]" country="United States of America" name="North Carolina" pageId="10" pageNumber="137">North Carolina</collectingRegion>
. The 
<typeStatus box="[1246,1298,1812,1836]" pageId="10" pageNumber="137">type</typeStatus>
locality, Helton Creek drains the southwest side of Mount Rogers, the highest mountain in 
<collectingRegion box="[574,671,1813,1837]" country="United States of America" name="Virginia" pageId="11" pageNumber="138">Virginia</collectingRegion>
at 
<quantity box="[706,796,1813,1837]" metricMagnitude="3" metricUnit="m" metricValue="1.746" pageId="11" pageNumber="138" unit="m" value="1746.0">1,746 m</quantity>
(
<quantity box="[196,294,1849,1873]" metricMagnitude="3" metricUnit="m" metricValue="1.7461992" pageId="11" pageNumber="138" unit="ft" value="5729.0">5,729 ft.</quantity>
), and has a diverse stonefly fauna. Other adult stoneflies collected with the new species at the type locality were 
<taxonomicName authority="Ricker, 1936" authorityName="Ricker" authorityYear="1936" box="[1073,1439,1777,1801]" class="Insecta" family="Chloroperlidae" genus="Alloperla" kingdom="Animalia" order="Plecoptera" pageId="11" pageNumber="138" phylum="Arthropoda" rank="species" species="concolor">
<emphasis box="[1073,1275,1777,1801]" italics="true" pageId="11" pageNumber="138">Alloperla concolor</emphasis>
Ricker, 1936
</taxonomicName>
, 
<taxonomicName authority="(Claassen, 1936)" baseAuthorityName="Claassen" baseAuthorityYear="1936" box="[835,1247,1813,1837]" class="Insecta" family="Nemouridae" genus="Amphinemura" kingdom="Animalia" order="Plecoptera" pageId="11" pageNumber="138" phylum="Arthropoda" rank="species" species="wui">
<emphasis box="[835,1043,1813,1837]" italics="true" pageId="11" pageNumber="138">Amphinemura wui</emphasis>
(Claassen, 1936)
</taxonomicName>
, 
<taxonomicName authority="(Banks, 1920)" baseAuthorityName="Banks" baseAuthorityYear="1920" class="Insecta" family="Perlodidae" genus="Cultus" kingdom="Animalia" order="Plecoptera" pageId="11" pageNumber="138" phylum="Arthropoda" rank="species" species="verticalis">
<emphasis box="[1261,1442,1813,1837]" italics="true" pageId="11" pageNumber="138">Cultus verticalis</emphasis>
(Banks, 1920)
</taxonomicName>
, 
<taxonomicName authority="Kirchner &amp; Kondratieff, 2005" authorityName="Kirchner &amp; Kondratieff" authorityYear="2005" class="Insecta" family="Chloroperlidae" genus="Haploperla" kingdom="Animalia" order="Plecoptera" pageId="11" pageNumber="138" phylum="Arthropoda" rank="species" species="parkeri">
<emphasis box="[1045,1265,1849,1873]" italics="true" pageId="11" pageNumber="138">Haploperla parkeri</emphasis>
Kirchner &amp; Kondratieff, 2005
</taxonomicName>
, 
<taxonomicName authority="Ricker, 1952" authorityName="Ricker" authorityYear="1952" box="[1075,1405,1885,1909]" class="Insecta" family="Perlodidae" genus="Isoperla" kingdom="Animalia" order="Plecoptera" pageId="11" pageNumber="138" phylum="Arthropoda" rank="species" species="cotta">
<emphasis box="[1075,1230,1885,1909]" italics="true" pageId="11" pageNumber="138">Isoperla cotta</emphasis>
Ricker, 1952
</taxonomicName>
, 
<taxonomicName authority="Verdone &amp; Kondratieff, 2017" authorityName="Verdone &amp; Kondratieff" authorityYear="2017" class="Insecta" family="Perlodidae" genus="Isoperla" kingdom="Animalia" lastPageId="12" lastPageNumber="139" order="Plecoptera" pageId="11" pageNumber="138" phylum="Arthropoda" rank="species" species="dewalti">
<emphasis italics="true" lastPageId="12" lastPageNumber="139" pageId="11" pageNumber="138">I. dewalti</emphasis>
Verdone &amp; Kondratieff, 2017
</taxonomicName>
, 
<taxonomicName authority="Szczytko &amp; Kondratieff, 2015" authorityName="Szczytko &amp; Kondratieff" authorityYear="2015" class="Insecta" family="Perlodidae" genus="Isoperla" kingdom="Animalia" order="Plecoptera" pageId="12" pageNumber="139" phylum="Arthropoda" rank="species" species="reesi">
<emphasis box="[710,798,229,252]" italics="true" pageId="12" pageNumber="139">I. reesi</emphasis>
Szczytko &amp; Kondratieff, 2015
</taxonomicName>
, 
<taxonomicName authority="Claassen, 1923" authorityName="Claassen" authorityYear="1923" class="Insecta" family="Leuctridae" genus="Leuctra" kingdom="Animalia" order="Plecoptera" pageId="12" pageNumber="139" phylum="Arthropoda" rank="species" species="duplicata">
<emphasis box="[597,797,264,288]" italics="true" pageId="12" pageNumber="139">Leuctra duplicata</emphasis>
Claassen, 1923
</taxonomicName>
, 
<taxonomicName authority="(Walker, 1852)" baseAuthorityName="Walker" baseAuthorityYear="1852" box="[398,753,300,324]" class="Insecta" family="Leuctridae" genus="Leuctra" kingdom="Animalia" order="Plecoptera" pageId="12" pageNumber="139" phylum="Arthropoda" rank="species" species="ferruginea">
<emphasis box="[398,549,300,324]" italics="true" pageId="12" pageNumber="139">L. ferruginea</emphasis>
(Walker, 1852)
</taxonomicName>
, 
<taxonomicName authority="Banks, 1906" authorityName="Banks" authorityYear="1906" class="Insecta" family="Leuctridae" genus="Leuctra" kingdom="Animalia" order="Plecoptera" pageId="12" pageNumber="139" phylum="Arthropoda" rank="species" species="grandis">
<emphasis italics="true" pageId="12" pageNumber="139">L. grandis</emphasis>
Banks, 1906
</taxonomicName>
, 
<taxonomicName authority="Claassen, 1923" authorityName="Claassen" authorityYear="1923" box="[473,793,336,360]" class="Insecta" family="Leuctridae" genus="Leuctra" kingdom="Animalia" order="Plecoptera" pageId="12" pageNumber="139" phylum="Arthropoda" rank="species" species="sibleyi">
<emphasis box="[473,583,336,360]" italics="true" pageId="12" pageNumber="139">L. sibleyi</emphasis>
Claassen, 1923
</taxonomicName>
, 
<taxonomicName authority="(Banks, 1920)" baseAuthorityName="Banks" baseAuthorityYear="1920" box="[189,643,372,396]" class="Insecta" family="Perlodidae" genus="Malirekus" kingdom="Animalia" order="Plecoptera" pageId="12" pageNumber="139" phylum="Arthropoda" rank="species" species="hastatus">
<emphasis box="[189,423,372,396]" italics="true" pageId="12" pageNumber="139">Malirekus hastatus</emphasis>
(Banks, 1920)
</taxonomicName>
, 
<taxonomicName authority="(Walker, 1852)" baseAuthorityName="Walker" baseAuthorityYear="1852" class="Insecta" family="Nemouridae" genus="Ostrocerca" kingdom="Animalia" order="Plecoptera" pageId="12" pageNumber="139" phylum="Arthropoda" rank="species" species="albidipennis">
<emphasis italics="true" pageId="12" pageNumber="139">Ostrocerca albidipennis</emphasis>
(Walker, 1852)
</taxonomicName>
, 
<taxonomicName authority="(Claassen, 1937)" baseAuthorityName="Claassen" baseAuthorityYear="1937" class="Insecta" family="Leuctridae" genus="Paraleuctra" kingdom="Animalia" order="Plecoptera" pageId="12" pageNumber="139" phylum="Arthropoda" rank="species" species="sara">
<emphasis box="[594,797,408,432]" italics="true" pageId="12" pageNumber="139">Paraleuctra sara</emphasis>
(Claassen, 1937)
</taxonomicName>
, 
<taxonomicName authority="Ricker, 1952" authorityName="Ricker" authorityYear="1952" box="[414,793,444,468]" class="Insecta" family="Pteronarcyidae" genus="Pteronarcys" kingdom="Animalia" order="Plecoptera" pageId="12" pageNumber="139" phylum="Arthropoda" rank="species" species="scotti">
<emphasis box="[414,619,445,468]" italics="true" pageId="12" pageNumber="139">Pteronarcys scotti</emphasis>
Ricker, 1952
</taxonomicName>
, 
<taxonomicName authority="(Banks, 1911)" baseAuthorityName="Banks" baseAuthorityYear="1911" box="[189,552,480,504]" class="Insecta" family="Chloroperlidae" genus="Sweltsa" kingdom="Animalia" order="Plecoptera" pageId="12" pageNumber="139" phylum="Arthropoda" rank="species" species="lateralis">
<emphasis box="[189,372,480,504]" italics="true" pageId="12" pageNumber="139">Sweltsa lateralis</emphasis>
(Banks, 1911)
</taxonomicName>
, 
<taxonomicName authority="(Banks, 1911)" baseAuthorityName="Banks" baseAuthorityYear="1911" class="Insecta" family="Chloroperlidae" genus="Sweltsa" kingdom="Animalia" order="Plecoptera" pageId="12" pageNumber="139" phylum="Arthropoda" rank="species" species="mediana">
<emphasis box="[571,696,480,504]" italics="true" pageId="12" pageNumber="139">S. mediana</emphasis>
(Banks, 1911)
</taxonomicName>
, 
<taxonomicName authority="(Needham &amp; Smith, 1916)" baseAuthorityName="Needham &amp; Smith" baseAuthorityYear="1916" box="[268,761,516,540]" class="Insecta" family="Peltoperlidae" genus="Tallaperla" kingdom="Animalia" order="Plecoptera" pageId="12" pageNumber="139" phylum="Arthropoda" rank="species" species="anna">
<emphasis box="[268,438,516,540]" italics="true" pageId="12" pageNumber="139">Tallaperla anna</emphasis>
(Needham &amp; Smith, 1916)
</taxonomicName>
, 
<taxonomicName authority="Kondratieff, Kirchner &amp; Zuellig, 2007" authorityName="Kondratieff, Kirchner &amp; Zuellig" authorityYear="2007" class="Insecta" family="Peltoperlidae" genus="Tallaperla" kingdom="Animalia" order="Plecoptera" pageId="12" pageNumber="139" phylum="Arthropoda" rank="species" species="maiyae">
<emphasis italics="true" pageId="12" pageNumber="139">T. maiyae</emphasis>
Kondratieff, Kirchner &amp; Zuellig, 2007
</taxonomicName>
, and 
<taxonomicName authority="(Needham &amp; Smith, 1916)" baseAuthorityName="Needham &amp; Smith" baseAuthorityYear="1916" class="Insecta" family="Peltoperlidae" genus="Tallaperla" kingdom="Animalia" order="Plecoptera" pageId="12" pageNumber="139" phylum="Arthropoda" rank="species" species="maria">
<emphasis italics="true" pageId="12" pageNumber="139">T. maria</emphasis>
(Needham &amp; Smith, 1916)
</taxonomicName>
.
</paragraph>
</subSubSection>
<caption ID-DOI="http://doi.org/10.5281/zenodo.4757855" ID-Zenodo-Dep="4757855" httpUri="https://zenodo.org/record/4757855/files/figure.png" pageId="11" pageNumber="138" startId="11.[189,226,1633,1657]" targetBox="[286,1344,218,1587]" targetPageId="11">
<paragraph blockId="11.[189,1442,1633,1693]" pageId="11" pageNumber="138">Fig. 9. Distribution map of examined material and physiographic provinces. See text for comprehensive list of states occupied for some species.</paragraph>
</caption>
<subSubSection pageId="12" pageNumber="139" type="discussion">
<paragraph blockId="12.[189,798,228,1079]" pageId="12" pageNumber="139">
<emphasis bold="true" box="[189,330,623,647]" pageId="12" pageNumber="139">Discussion.</emphasis>
<bibRefCitation author="Kondratieff, B. C." box="[361,598,623,648]" pageId="12" pageNumber="139" pagination="1 - 192" refId="ref8569" refString="Kondratieff, B. C. 2004. Perlodidae - Perlodinae (The Springflies). Pp. 149 - 180. In: B. P. Stark &amp; B. J. Armitage (eds.). The stoneflies (Plecoptera) of eastern North America. Volume II. Chloroperlidae, Perlidae, and Perlodidae). Ohio Biological Survey Bulletin New Series Volume 14 (4): 1 - 192." type="journal article" year="2004">Kondratieff (2004)</bibRefCitation>
provided an illustration of the lateral stylets of 
<taxonomicName authorityName="Claassen" authorityYear="1923" box="[606,742,660,684]" class="Insecta" family="Leuctridae" genus="Leuctra" kingdom="Animalia" order="Plecoptera" pageId="12" pageNumber="139" phylum="Arthropoda" rank="species" species="duplicata">
<emphasis box="[606,742,660,684]" italics="true" pageId="12" pageNumber="139">D. duplicata</emphasis>
</taxonomicName>
(fig. 8.84) from a specimen collected from Big Otter River, Bedford County, 
<collectingRegion box="[493,593,732,756]" country="United States of America" name="Virginia" pageId="12" pageNumber="139">Virginia</collectingRegion>
, which was not accurately illustrated. No specimens of 
<taxonomicName authorityName="Claassen" authorityYear="1923" box="[662,797,768,792]" class="Insecta" family="Leuctridae" genus="Leuctra" kingdom="Animalia" order="Plecoptera" pageId="12" pageNumber="139" phylum="Arthropoda" rank="species" species="duplicata">
<emphasis box="[662,797,768,792]" italics="true" pageId="12" pageNumber="139">D. duplicata</emphasis>
</taxonomicName>
examined in this study, including the specimen from Big Otter River, have lateral stylet apices with a dorsal expansion projecting markedly beyond the basal plane. In several male specimens of 
<taxonomicName authorityName="Claassen" authorityYear="1923" class="Insecta" family="Leuctridae" genus="Leuctra" kingdom="Animalia" order="Plecoptera" pageId="12" pageNumber="139" phylum="Arthropoda" rank="species" species="duplicata">
<emphasis italics="true" pageId="12" pageNumber="139">D. duplicata</emphasis>
</taxonomicName>
from 
<collectingRegion box="[367,553,948,972]" country="United States of America" name="North Carolina" pageId="12" pageNumber="139">North Carolina</collectingRegion>
, the head pattern is similar to the new species, but the lateral stylets clearly lack the characteristic dorsal expansion of 
<taxonomicName authorityName="Kondratieff &amp; Verdone" authorityYear="2017" class="Insecta" family="Perlodidae" genus="Diploperla" kingdom="Animalia" order="Plecoptera" pageId="12" pageNumber="139" phylum="Arthropoda" rank="species" species="janeae">
<emphasis italics="true" pageId="12" pageNumber="139">D. janeae</emphasis>
</taxonomicName>
.
</paragraph>
</subSubSection>
</treatment>
</document>