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<document id="3897F7E3F7896740A1EDBE49D23D196E" ID-DOI="10.11646/phytotaxa.642.2.2" ID-ISSN="1179-3163" ID-Zenodo-Dep="13214245" IM.bibliography_approvedBy="felipe" IM.illustrations_approvedBy="felipe" IM.materialsCitations_approvedBy="felipe" IM.tables_requiresApprovalFor="GgImagineBatch" IM.taxonomicNames_approvedBy="felipe" IM.treatmentCitations_approvedBy="felipe" IM.treatments_approvedBy="felipe" checkinTime="1722602361337" checkinUser="felipe" docAuthor="Yang, Yan-Yan, Thiyagaraja, Vinodhini, Wanasinghe, Dhanushka N., Ar, Gomes De Farias &amp; Zhao, Qi" docDate="2024" docId="039F879EFFEFFFBD31F5184CFC15FBC9" docLanguage="en" docName="phytotaxa.642.2.2.pdf" docOrigin="Phytotaxa 642 (2)" docSource="http://dx.doi.org/10.11646/phytotaxa.642.2.2" docStyle="DocumentStyle:96748F8F1B6C902996E134952A3A36B9.13:Phytotaxa.2014-.journal_article" docStyleId="96748F8F1B6C902996E134952A3A36B9" docStyleName="Phytotaxa.2014-.journal_article" docStyleVersion="13" docTitle="Neomanoharachariella xizangensis Y. Y. Yang &amp; Q. Zhao, sp. nov." docType="treatment" docVersion="2" lastPageNumber="134" masterDocId="FFA6FFE6FFEAFFBA317D1F58FFC4FFA9" masterDocTitle="Phylogenetic and taxonomic appraisal of Neomanoharachariella xizangensis sp. nov. and the first asexual report of Acanthostigmina (Tubeufiaceae, Tubeufiales) from Xizang Autonomous Region, China" masterLastPageNumber="140" masterPageNumber="127" pageNumber="132" updateTime="1722804692060" updateUser="ExternalLinkService" zenodo-license-figures="UNSPECIFIED" zenodo-license-treatments="UNSPECIFIED">
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<mods:titleInfo id="E64BC8C4ED3457305A4BFF91D01D265E">
<mods:title id="1F24B745AB1FF7794B4D7DF5A9E40030">Phylogenetic and taxonomic appraisal of Neomanoharachariella xizangensis sp. nov. and the first asexual report of Acanthostigmina (Tubeufiaceae, Tubeufiales) from Xizang Autonomous Region, China</mods:title>
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<mods:roleTerm id="DCCF0EED69B4E39A0F9D248FE97E33AE">Author</mods:roleTerm>
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<mods:namePart id="A26709BACA40C683F8A062F95665719D">Yang, Yan-Yan</mods:namePart>
<mods:nameIdentifier id="8AEA57CEA273C509CA7263DCDA56ADD3" type="ORCID">0009-0007-9521-558X</mods:nameIdentifier>
<mods:affiliation id="5A4E7E777D24927B710D49EF0BE2C4E6">CAS Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Panlong District, Kunming, Yunnan, 650201, P. R. China &amp; Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand &amp; School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand</mods:affiliation>
<mods:nameIdentifier id="198C50C59961305740AD5DCE8F85BA44" type="email">6471105019@lamduan.mfu.ac.th</mods:nameIdentifier>
</mods:name>
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<mods:namePart id="C6E886115D640D01AE6C883E66852AB3">Thiyagaraja, Vinodhini</mods:namePart>
<mods:nameIdentifier id="E71BB9E395DE0B0752353FCC93B4F826" type="ORCID">0000-0002-8091-4579</mods:nameIdentifier>
<mods:affiliation id="E45FD7B1F09D7765AC8877A112EB999E">CAS Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Panlong District, Kunming, Yunnan, 650201, P. R. China</mods:affiliation>
<mods:nameIdentifier id="41541D639B2584F8CC8E82AB423D534D" type="email">vino.thiyagaraja@gmail.com</mods:nameIdentifier>
</mods:name>
<mods:name id="4368E53E79B732A8E7015200DCBB40FB" type="personal">
<mods:role id="7ABBD3317294DDCA09D5E3E1EE74B609">
<mods:roleTerm id="F39EAFD52ED9049AE1D7F7B3D7D4DE15">Author</mods:roleTerm>
</mods:role>
<mods:namePart id="676D2789B80CAE33333AFA08D79EB158">Wanasinghe, Dhanushka N.</mods:namePart>
<mods:nameIdentifier id="EAF747525BCA62CB123BB2F2790DD926" type="ORCID">0000-0003-1759-3933</mods:nameIdentifier>
<mods:affiliation id="88A35C6F492A7C329D56803CB1D99211">Honghe Center for Mountain Futures, Kunming Institute of Botany, Chinese Academy of Sciences, Honghe County 654400, Yunnan Province, P. R. China</mods:affiliation>
<mods:nameIdentifier id="ECFCCEC26DC2DBC1F6BCCC457222F2A4" type="email">dnadeeshan@gmail.com</mods:nameIdentifier>
</mods:name>
<mods:name id="CC6EA981326BC0397EFA018DE6833EBB" type="personal">
<mods:role id="F144C8D1DA248B51DF03723BB2D218DF">
<mods:roleTerm id="F1380E3B6FBBC3E4440F8A29FA477437">Author</mods:roleTerm>
</mods:role>
<mods:namePart id="62359124611887024C082944201922F2">Ar, Gomes De Farias</mods:namePart>
<mods:nameIdentifier id="06027BAC2158E74F85683339C9C6BE39" type="ORCID">0000-0003-4768-1547</mods:nameIdentifier>
<mods:affiliation id="CD0927AA6D086F8511D038D0D2906E0C">Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand</mods:affiliation>
<mods:nameIdentifier id="C06E1AA1162F19F2EEAA9E6F8DEDC89E" type="email">rfariasagro@gmail.com</mods:nameIdentifier>
</mods:name>
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<mods:namePart id="22567B424A191E0FB2D14819FCC3EC70">Zhao, Qi</mods:namePart>
<mods:nameIdentifier id="0F6978973344B5B490C40A140F11D086" type="ORCID">0000-0001-8169-0573</mods:nameIdentifier>
<mods:affiliation id="6128CF256C9E717750184C908C9CB9C4">CAS Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Panlong District, Kunming, Yunnan, 650201, P. R. China</mods:affiliation>
<mods:nameIdentifier id="AA2CFAAC7CDD3BEBE4B5330D7F88442B" type="email">zhaoqi@mail.kib.ac.cn</mods:nameIdentifier>
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<mods:titleInfo id="69E38142C4B1BEF92C87CCA40C399196">
<mods:title id="9C34B4FCAEA40C6A497A7E911890A380">Phytotaxa</mods:title>
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<mods:part id="0186E5A6AB468ACFE4AD92DFFBE20C2B">
<mods:date id="C4C410EBF51A5890D3858074B9608909">2024</mods:date>
<mods:detail id="E2A59581148E90E0EF618945A44C60C1" type="pubDate">
<mods:number id="12AEBFC81EE25E25217B012DAFAEA548">2024-03-28</mods:number>
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<mods:detail id="BEAD9E14C8FFAB148E086488FB22B125" type="volume">
<mods:number id="D54462B8EA12DDB2A2457D72D4B184B1">642</mods:number>
</mods:detail>
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<mods:number id="F07457C34AF734FAF89C2A0309B72A9B">2</mods:number>
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<mods:url id="04E6C4AA7B0DD43A6F9F444E3E12F7EB">http://dx.doi.org/10.11646/phytotaxa.642.2.2</mods:url>
</mods:location>
<mods:classification id="16D19AC5987763DEE5CE0D763FD53010">journal article</mods:classification>
<mods:identifier id="F79EB1CE3E44A6247D2C10B65F2A357D" type="DOI">10.11646/phytotaxa.642.2.2</mods:identifier>
<mods:identifier id="2B8C5881C06452A3EF4982AD5741B21D" type="ISSN">1179-3163</mods:identifier>
<mods:identifier id="16F346711FB67CB0AA5818E6E0AD14E2" type="Zenodo-Dep">13214245</mods:identifier>
</mods:mods>
<treatment id="039F879EFFEFFFBD31F5184CFC15FBC9" LSID="urn:lsid:plazi:treatment:039F879EFFEFFFBD31F5184CFC15FBC9" httpUri="http://treatment.plazi.org/id/039F879EFFEFFFBD31F5184CFC15FBC9" lastPageId="7" lastPageNumber="134" pageId="5" pageNumber="132">
<subSubSection id="C32C6503FFEFFFBF31F5184CFC61F8FA" pageId="5" pageNumber="132" type="nomenclature">
<paragraph id="8B893688FFEFFFBF31F5184CFC10F887" blockId="5.[136,980,1812,1839]" box="[136,980,1812,1839]" pageId="5" pageNumber="132">
<heading id="D0C181E4FFEFFFBF31F5184CFC10F887" box="[136,980,1812,1839]" fontSize="11" level="2" pageId="5" pageNumber="132" reason="3">
<taxonomicName id="4C364D0BFFEFFFBF31F5184CFCDAF887" authority="Y. Y. Yang &amp; Q. Zhao" authorityName="Y. Y. Yang &amp; Q. Zhao" box="[136,798,1812,1839]" class="Dothideomycetes" family="Tubeufiaceae" genus="Neomanoharachariella" higherTaxonomySource="CoL,GBIF" kingdom="Fungi" order="Tubeufiales" pageId="5" pageNumber="132" phylum="Ascomycota" rank="species" species="xizangensis" status="sp. nov.">
<emphasis id="B942EA9AFFEFFFBF31F5184CFDE6F887" bold="true" box="[136,546,1812,1839]" italics="true" pageId="5" pageNumber="132">Neomanoharachariella xizangensis</emphasis>
Y. Y. Yang &amp; Q. Zhao
</taxonomicName>
<emphasis id="B942EA9AFFEFFFBF3258184DFCBDF887" box="[805,889,1813,1838]" italics="true" pageId="5" pageNumber="132">
<taxonomicNameLabel id="A27157E1FFEFFFBF3258184DFCBDF887" box="[805,889,1813,1838]" pageId="5" pageNumber="132" rank="species">sp. nov.</taxonomicNameLabel>
</emphasis>
(
<figureCitation id="130D2A0DFFEFFFBF32F5184CFC0FF887" box="[904,971,1812,1838]" captionStart="FIGURE 2" captionStartId="6.[136,234,1953,1975]" captionTargetBox="[179,1407,187,1925]" captionTargetId="figure-25@6.[179,1407,187,1925]" captionTargetPageId="6" captionText="FIGURE 2. Neomanoharachariella xizangensis (HKAS 131153, holotype) a Host substrate. b Appearance of colonies on the substrate. cf Conidiophores and conidia. gh, l Conidiogenous cells and conidia. ik Conidia. m Germinated conidium. n, o Front and reverse colonies on PDA. Scale bars: cf, m = 50 μm, gl = 20 μm." figureDoi="http://doi.org/10.5281/zenodo.13214249" httpUri="https://zenodo.org/record/13214249/files/figure.png" pageId="5" pageNumber="132">Fig. 2</figureCitation>
)
</heading>
</paragraph>
<paragraph id="8B893688FFEFFFBF31F51860FC61F8FA" blockId="5.[136,933,1848,1875]" box="[136,933,1848,1875]" pageId="5" pageNumber="132">Index Fungorum number: IF 901427, Facesofungi number: FOF 15182</paragraph>
</subSubSection>
<subSubSection id="C32C6503FFEFFFBD31F518D8FC15FBC9" lastPageId="7" lastPageNumber="134" pageId="5" pageNumber="132" type="etymology">
<paragraph id="8B893688FFEFFFBF31F518D8FE1FF816" blockId="5.[136,1442,1920,1983]" pageId="5" pageNumber="132">
Etymology: Referring to the collection site “
<collectingRegion id="49F2F86AFFEFFFBF330418D8FD08F833" box="[633,716,1920,1946]" country="China" name="Xizang" pageId="5" pageNumber="132">Xizang</collectingRegion>
Autonomous Region,
<collectingCountry id="F3217618FFEFFFBF32B518D8FBD4F833" box="[968,1040,1920,1946]" name="China" pageId="5" pageNumber="132">China</collectingCountry>
”, where the
<typeStatus id="548D882AFFEFFFBF35E718D8FB38F833" box="[1178,1276,1920,1946]" pageId="5" pageNumber="132" type="holotype">holotype</typeStatus>
was collected.
<typeStatus id="548D882AFFEFFFBF31C018FCFEEEF817" box="[189,298,1956,1982]" pageId="5" pageNumber="132" type="holotype">Holotype</typeStatus>
: HKAS 131153
</paragraph>
<caption id="DF496600FFECFFBC31F518F9FD00F857" ID-DOI="http://doi.org/10.5281/zenodo.13214249" ID-Zenodo-Dep="13214249" httpUri="https://zenodo.org/record/13214249/files/figure.png" pageId="6" pageNumber="133" startId="6.[136,234,1953,1975]" targetBox="[179,1407,187,1925]" targetPageId="6" targetType="figure">
<paragraph id="8B893688FFECFFBC31F518F9FD00F857" blockId="6.[136,1452,1953,2047]" pageId="6" pageNumber="133">
<emphasis id="B942EA9AFFECFFBC31F518F9FEC7F81F" bold="true" box="[136,259,1953,1975]" pageId="6" pageNumber="133">FIGURE 2.</emphasis>
<taxonomicName id="4C364D0BFFECFFBC307718F9FD9BF81F" box="[266,607,1953,1974]" class="Dothideomycetes" family="Tubeufiaceae" genus="Neomanoharachariella" higherTaxonomySource="CoL,GBIF" kingdom="Fungi" order="Tubeufiales" pageId="6" pageNumber="133" phylum="Ascomycota" rank="species" species="xizangensis">
<emphasis id="B942EA9AFFECFFBC307718F9FD9BF81F" box="[266,607,1953,1974]" italics="true" pageId="6" pageNumber="133">Neomanoharachariella xizangensis</emphasis>
</taxonomicName>
(HKAS 131153, holotype) a Host substrate. b Appearance of colonies on the substrate. cf Conidiophores and conidia. gh, l Conidiogenous cells and conidia. ik Conidia. m Germinated conidium. n, o Front and reverse colonies on PDA. Scale bars: cf, m = 50 μm, gl = 20 μm.
</paragraph>
</caption>
<paragraph id="8B893688FFEDFFBD31C01FC6FB72FE70" blockId="7.[136,1452,158,1121]" pageId="7" pageNumber="134">
<emphasis id="B942EA9AFFEDFFBD31C01FC6FEE5FF11" box="[189,289,158,184]" italics="true" pageId="7" pageNumber="134">Saprobic</emphasis>
on the dead wood of an unidentified plant.
<emphasis id="B942EA9AFFEDFFBD33901FC6FC5FFF11" bold="true" box="[749,923,158,184]" pageId="7" pageNumber="134">Sexual morph:</emphasis>
unidentified.
<emphasis id="B942EA9AFFEDFFBD354C1FC6FB2AFF11" bold="true" box="[1073,1262,158,184]" pageId="7" pageNumber="134">Asexual morph:</emphasis>
<emphasis id="B942EA9AFFEDFFBD358F1FC6FA61FF11" box="[1266,1445,158,184]" italics="true" pageId="7" pageNumber="134">Hyphomycetous</emphasis>
.
<emphasis id="B942EA9AFFEDFFBD31F51F9AFF28FF75" box="[136,236,194,220]" italics="true" pageId="7" pageNumber="134">Colonies</emphasis>
on the natural substratum superficial, effuse, black, with dictyosporous.
<emphasis id="B942EA9AFFEDFFBD35581F9AFB57FF75" box="[1061,1171,194,220]" italics="true" pageId="7" pageNumber="134">Mycelium</emphasis>
partly superficial, partly immersed, and dark brown.
<emphasis id="B942EA9AFFEDFFBD30B11FBEFDB5FEA9" box="[460,625,230,256]" italics="true" pageId="7" pageNumber="134">Conidiophores</emphasis>
93163 µm × 3.95.5 µm (x̄ = 122 × 4.5 µm, n = 6), macronematous, mononematous, flexuous, unbranched, septate, pale brown, smooth-walled.
<emphasis id="B942EA9AFFEDFFBD32AC1E53FBCAFE8C" box="[977,1038,267,293]" italics="true" pageId="7" pageNumber="134">Setae</emphasis>
scattered, mixed with conidiophores, 92117 µm × 3.14.8 µm (x̄ = 103 × 4 µm, n = 8), dark brown, hyaline at the apex, straight, 45-septate, solitary, a long cylindrical stipe and a cuspate neck, smooth-walled.
<emphasis id="B942EA9AFFEDFFBD326F1E0AFC32FEC5" box="[786,1014,338,364]" italics="true" pageId="7" pageNumber="134">Conidiogenous cells</emphasis>
1523 µm × 3.24.9 µm (x̄ = 18 × 4.2 µm, n = 8), terminal, monoblastic, integrated, pale brown to hyaline, smooth-walled, cylindrical.
<emphasis id="B942EA9AFFEDFFBD35BF1E2EFAD8FE39" box="[1218,1308,374,400]" italics="true" pageId="7" pageNumber="134">Conidia</emphasis>
3475 µm × 1331 µm (x̄ = 54 × 24 µm, n = 26), long oval or ellipsoidal, muriform, shiny, pale brown when immature, becoming dark with black neoplasm when mature, 810-transversely septate, with 25-longitudinal septa.
</paragraph>
<paragraph id="8B893688FFEDFFBD31C01EBAFB25FDED" blockId="7.[136,1452,158,1121]" pageId="7" pageNumber="134">
<emphasis id="B942EA9AFFEDFFBD31C01EBAFE79FE55" box="[189,445,482,508]" italics="true" pageId="7" pageNumber="134">Culture characteristics</emphasis>
:
<emphasis id="B942EA9AFFEDFFBD30B71EBAFDE0FE55" box="[458,548,482,508]" italics="true" pageId="7" pageNumber="134">Conidia</emphasis>
germinating on PDA and germ tubes produced from conidia within 12 h.
<emphasis id="B942EA9AFFEDFFBD34351EBAFA68FE55" box="[1352,1452,482,508]" italics="true" pageId="7" pageNumber="134">Colonies</emphasis>
growing on PDA, subcircular, edge undulate, with a flat surface, sparse, reaching
<quantity id="4CCE9B6DFFEDFFBD35521D5EFB44FD88" box="[1071,1152,518,545]" metricMagnitude="-2" metricUnit="m" metricValue="1.8" pageId="7" pageNumber="134" unit="mm" value="18.0">18 mm</quantity>
in 20 days at 25 ℃, front white to brown in PDA medium, mycelium superficial and partially immersed, reverse dark brown.
</paragraph>
<paragraph id="8B893688FFEDFFBD31C01D16FEA0FD50" blockId="7.[136,1452,158,1121]" pageId="7" pageNumber="134">
<emphasis id="B942EA9AFFEDFFBD31C01D16FE52FDC1" box="[189,406,590,616]" italics="true" pageId="7" pageNumber="134">Material examined</emphasis>
:
<materialsCitation id="3B5E3CD5FFEDFFBD30D71D16FE3EFD18" collectingDate="2022-07-10" collectionCode="HKAS, KUNCC" country="China" county="Metuo County" latitude="29.14426" location="Nyingchi Prefecture" longLatPrecision="1" longitude="95.1955" municipality="Autonomous Region" pageId="7" pageNumber="134" specimenCode="HKAS 131153, KUNCC 23-15799" specimenCount="1" stateProvince="Xizang" typeStatus="holotype">
<collectingCountry id="F3217618FFEDFFBD30D71D16FE34FDC1" box="[426,496,590,616]" name="China" pageId="7" pageNumber="134">China</collectingCountry>
,
<collectingRegion id="49F2F86AFFEDFFBD337D1D16FD95FDC1" box="[512,593,590,616]" country="China" name="Xizang" pageId="7" pageNumber="134">Xizang</collectingRegion>
<collectingMunicipality id="6BEDACF2FFEDFFBD33261D16FC8FFDC1" box="[603,843,590,616]" pageId="7" pageNumber="134">Autonomous Region</collectingMunicipality>
,
<location id="8EE96053FFEDFFBD32261D16FB81FDC1" LSID="urn:lsid:plazi:treatment:039F879EFFEFFFBD31F5184CFC15FBC9:8EE96053FFEDFFBD32261D16FB81FDC1" box="[859,1093,590,616]" country="China" county="Metuo County" latitude="29.14426" longLatPrecision="1" longitude="95.1955" municipality="Autonomous Region" name="Nyingchi Prefecture" pageId="7" pageNumber="134" stateProvince="Xizang">Nyingchi Prefecture</location>
,
<collectingCounty id="62E84E04FFEDFFBD35281D17FB3AFDC1" box="[1109,1278,590,617]" pageId="7" pageNumber="134">Metuo County</collectingCounty>
,
<geoCoordinate id="EE02504FFFEDFFBD34701D16FA63FDC0" box="[1293,1447,590,617]" degrees="29.144261" direction="north" orientation="latitude" pageId="7" pageNumber="134" precision="1" value="29.14426">29.144261 N</geoCoordinate>
,
<geoCoordinate id="EE02504FFFEDFFBD31F51D2AFED2FD24" box="[136,278,626,653]" degrees="95.195501" direction="east" orientation="longitude" pageId="7" pageNumber="134" precision="1" value="95.1955">95.195501 E</geoCoordinate>
, on an unidentified dead wood.
<date id="FF881048FFEDFFBD33071D2AFCC9FD25" box="[634,781,626,652]" pageId="7" pageNumber="134" value="2022-07-10">
<collectingDate id="EFCCE9A0FFEDFFBD33071D2AFCC9FD25" box="[634,781,626,652]" pageId="7" pageNumber="134" value="2022-07-10">10 July 2022</collectingDate>
</date>
, Yanyan Yang, YYY318 (
<typeStatus id="548D882AFFEDFFBD35571D2AFB56FD25" box="[1066,1170,626,652]" pageId="7" pageNumber="134" type="holotype">holotype</typeStatus>
:
<specimenCode id="DB909EF3FFEDFFBD35E61D2AFA81FD24" box="[1179,1349,626,653]" collectionCode="HKAS" country="China" httpUri="http://grbio.org/cool/di2a-40jb" name="Cryptogamic Herbarium of Kunming Institute of Botany" pageId="7" pageNumber="134">HKAS 131153</specimenCode>
), ex-type living culture
<specimenCode id="DB909EF3FFEDFFBD305E1DCEFE3EFD18" box="[291,506,662,689]" collectionCode="KUNCC" pageId="7" pageNumber="134">KUNCC 23-15799</specimenCode>
</materialsCitation>
;
<materialsCitation id="3B5E3CD5FFEDFFBD33781DCEFEA4FD50" collectingDate="2022-07-13" collectionCode="HKAS, KUNCC" country="China" county="Bome County" latitude="30.396643" location="Nyingchi Prefecture" longLatPrecision="1" longitude="94.34493" municipality="Autonomous Region" pageId="7" pageNumber="134" specimenCode="HKAS 131156, KUNCC 23-15800" specimenCount="1" stateProvince="Xizang" typeStatus="holotype">
<collectingCountry id="F3217618FFEDFFBD33781DCEFD8FFD19" box="[517,587,662,688]" name="China" pageId="7" pageNumber="134">China</collectingCountry>
,
<collectingRegion id="49F2F86AFFEDFFBD33281DCEFD62FD19" box="[597,678,662,688]" country="China" name="Xizang" pageId="7" pageNumber="134">Xizang</collectingRegion>
<collectingMunicipality id="6BEDACF2FFEDFFBD33D71DCEFC51FD19" box="[682,917,662,688]" pageId="7" pageNumber="134">Autonomous Region</collectingMunicipality>
,
<location id="8EE96053FFEDFFBD32E21DCEFB47FD19" LSID="urn:lsid:plazi:treatment:039F879EFFEFFFBD31F5184CFC15FBC9:8EE96053FFEDFFBD32E21DCEFB47FD19" box="[927,1155,662,688]" country="China" county="Bome County" latitude="30.396643" longLatPrecision="1" longitude="94.34493" municipality="Autonomous Region" name="Nyingchi Prefecture" pageId="7" pageNumber="134" stateProvince="Xizang">Nyingchi Prefecture</location>
,
<collectingCounty id="62E84E04FFEDFFBD35F31DCFFAEFFD19" box="[1166,1323,662,689]" pageId="7" pageNumber="134">Bome County</collectingCounty>
,
<geoCoordinate id="EE02504FFFEDFFBD34491DCEFF5BFD7C" degrees="30.396643" direction="north" orientation="latitude" pageId="7" pageNumber="134" precision="1" value="30.396643">30.396643 N</geoCoordinate>
,
<geoCoordinate id="EE02504FFFEDFFBD31D11DE2FEF9FD7C" box="[172,317,698,725]" degrees="94.344931" direction="east" orientation="longitude" pageId="7" pageNumber="134" precision="1" value="94.34493">94.344931 E</geoCoordinate>
, on unidentified dead wood,
<date id="FF881048FFEDFFBD33F21DE2FCE3FD7D" box="[655,807,698,724]" pageId="7" pageNumber="134" value="2022-07-13">
<collectingDate id="EFCCE9A0FFEDFFBD33F21DE2FCE3FD7D" box="[655,807,698,724]" pageId="7" pageNumber="134" value="2022-07-13">13 July 2022</collectingDate>
</date>
, Yanyan Yang, YYY318-2 (
<specimenCode id="DB909EF3FFEDFFBD35141DE2FAD1FD7C" box="[1129,1301,698,725]" collectionCode="HKAS" country="China" httpUri="http://grbio.org/cool/di2a-40jb" name="Cryptogamic Herbarium of Kunming Institute of Botany" pageId="7" pageNumber="134">HKAS 131156</specimenCode>
), live culture
<specimenCode id="DB909EF3FFEDFFBD31F51D86FEA4FD50" box="[136,352,734,761]" collectionCode="KUNCC" pageId="7" pageNumber="134">KUNCC 23-15800</specimenCode>
</materialsCitation>
.
</paragraph>
<paragraph id="8B893688FFEDFFBD31C01C5BFC15FBC9" blockId="7.[136,1452,158,1121]" pageId="7" pageNumber="134">
<emphasis id="B942EA9AFFEDFFBD31C01C5BFF39FCB5" box="[189,253,771,796]" italics="true" pageId="7" pageNumber="134">Notes</emphasis>
: In the ITS, LSU,
<emphasis id="B942EA9AFFEDFFBD30AF1C5AFDD8FCB5" box="[466,540,770,796]" italics="true" pageId="7" pageNumber="134">tef 1-α</emphasis>
, and
<emphasis id="B942EA9AFFEDFFBD33261C5AFD4BFCB5" box="[603,655,770,796]" italics="true" pageId="7" pageNumber="134">rpb2</emphasis>
combined phylogenetic analyses, our species formed a sister subclade to
<taxonomicName id="4C364D0BFFEDFFBD31D81C7EFDD7FCE9" box="[165,531,806,832]" class="Dothideomycetes" family="Tubeufiaceae" genus="Neomanoharachariella" higherTaxonomySource="CoL,GBIF" kingdom="Fungi" order="Tubeufiales" pageId="7" pageNumber="134" phylum="Ascomycota" rank="species" species="aquatica">
<emphasis id="B942EA9AFFEDFFBD31D81C7EFDD7FCE9" box="[165,531,806,832]" italics="true" pageId="7" pageNumber="134">Neomanoharachariella aquatica</emphasis>
</taxonomicName>
(CGMCC 3.23539) with high statistical support (100 ML/1.00 PP). The base pair comparison between
<taxonomicName id="4C364D0BFFEDFFBD30041C13FDD9FCCD" box="[377,541,843,868]" class="Dothideomycetes" family="Tubeufiaceae" genus="Neomanoharachariella" higherTaxonomySource="CoL,GBIF" kingdom="Fungi" order="Tubeufiales" pageId="7" pageNumber="134" phylum="Ascomycota" rank="species" species="xizangensis">
<emphasis id="B942EA9AFFEDFFBD30041C13FDD9FCCD" box="[377,541,843,868]" italics="true" pageId="7" pageNumber="134">N. xizangensis</emphasis>
</taxonomicName>
(HKAS 131153) and
<taxonomicName id="4C364D0BFFEDFFBD32671C13FC5AFCCD" box="[794,926,843,868]" class="Dothideomycetes" family="Tubeufiaceae" genus="Neomanoharachariella" higherTaxonomySource="CoL,GBIF" kingdom="Fungi" order="Tubeufiales" pageId="7" pageNumber="134" phylum="Ascomycota" rank="species" species="aquatica">
<emphasis id="B942EA9AFFEDFFBD32671C13FC5AFCCD" box="[794,926,843,868]" italics="true" pageId="7" pageNumber="134">N. aquatica</emphasis>
</taxonomicName>
(CGMCC 3.23539) revealed 92.66% for ITS, 98.89 % for LSU, 93.70 % for
<emphasis id="B942EA9AFFEDFFBD30AB1C36FDD9FC21" box="[470,541,878,904]" italics="true" pageId="7" pageNumber="134">tef 1-α</emphasis>
, and 93.33 % for
<emphasis id="B942EA9AFFEDFFBD33A01C36FCD5FC21" box="[733,785,878,904]" italics="true" pageId="7" pageNumber="134">rpb2</emphasis>
similarity. The new taxon shares similar characteristics to
<taxonomicName id="4C364D0BFFEDFFBD34EF1C37FF2EFC05" class="Dothideomycetes" family="Tubeufiaceae" genus="Neomanoharachariella" higherTaxonomySource="CoL,GBIF" kingdom="Fungi" order="Tubeufiales" pageId="7" pageNumber="134" phylum="Ascomycota" rank="species" species="aquatica">
<emphasis id="B942EA9AFFEDFFBD34EF1C37FF2EFC05" italics="true" pageId="7" pageNumber="134">N. aquatica</emphasis>
</taxonomicName>
in having cylindrical, integrated conidiogenous cells, forming muriform septate conidia. However,
<taxonomicName id="4C364D0BFFEDFFBD34561CCBFA6FFC05" box="[1323,1451,915,940]" class="Dothideomycetes" family="Tubeufiaceae" genus="Neomanoharachariella" higherTaxonomySource="CoL,GBIF" kingdom="Fungi" order="Tubeufiales" pageId="7" pageNumber="134" phylum="Ascomycota" rank="species" species="aquatica">
<emphasis id="B942EA9AFFEDFFBD34561CCBFA6FFC05" box="[1323,1451,915,940]" italics="true" pageId="7" pageNumber="134">N. aquatica</emphasis>
</taxonomicName>
has comparatively shorter (2031 × 3.54.2 µm) conidiophores that are straight or flexuous, paler, tuberculous at the top, smooth conidia, without setae (
<bibRefCitation id="EFA74B79FFEDFFBD33621C82FD04FC5D" author="Li, L. L. &amp; Shen, H. W. &amp; Bao, D. F. &amp; Wanasinghe, D. N. &amp; Lu, Y. Z. &amp; Feng, Y. &amp; Luo, Z. L." box="[543,704,986,1013]" pageId="7" pageNumber="134" refId="ref8570" refString="Li, L. L., Shen, H. W., Bao, D. F., Wanasinghe, D. N., Lu, Y. Z., Feng, Y. &amp; Luo, Z. L. (2022) The plethora of Tubeufiaceae in lakes of the northwestern Yunnan plateau, China. Frontiers in Microbiology 13: 1056669. https: // doi. org / 10.3389 / fmicb. 2022.1056669" type="journal volume" year="2022">
Li
<emphasis id="B942EA9AFFEDFFBD333F1C83FDB1FC5D" box="[578,629,986,1012]" italics="true" pageId="7" pageNumber="134">et al</emphasis>
. 2022
</bibRefCitation>
), whereas
<taxonomicName id="4C364D0BFFEDFFBD32471C83FC19FC5D" box="[826,989,987,1012]" class="Dothideomycetes" family="Tubeufiaceae" genus="Neomanoharachariella" higherTaxonomySource="CoL,GBIF" kingdom="Fungi" order="Tubeufiales" pageId="7" pageNumber="134" phylum="Ascomycota" rank="species" species="xizangensis">
<emphasis id="B942EA9AFFEDFFBD32471C83FC19FC5D" box="[826,989,987,1012]" italics="true" pageId="7" pageNumber="134">N. xizangensis</emphasis>
</taxonomicName>
has comparatively longer conidiophores (90160 × 45.5 µm) that are flexuous, septate, pale brown and black neoplasm conidia with straight setae. Based on the recommendation for species boundary delimitation by
<bibRefCitation id="EFA74B79FFEDFFBD326B1B7AFBD6FB95" author="Chethana, K. T. &amp; Manawasinghe, I. S. &amp; Hurdeal, V. G. &amp; Bhunjun, C. S. &amp; Appadoo, M. A. &amp; Gentekaki, E. &amp; Raspe, O. &amp; Promputtha, I. &amp; Hyde, K. D." box="[790,1042,1058,1085]" pageId="7" pageNumber="134" pagination="1 - 25" refId="ref7035" refString="Chethana, K. T., Manawasinghe, I. S., Hurdeal, V. G., Bhunjun, C. S., Appadoo, M. A., Gentekaki, E., Raspe, O., Promputtha, I. &amp; Hyde, K. D. (2021) What are fungal species and how to delineate them?. Fungal Diversity 109 (1): 1 - 25. https: // doi. org / 10.1007 / s 13225 - 021 - 00483 - 9" type="journal article" year="2021">
Chethana
<emphasis id="B942EA9AFFEDFFBD32FA1B7BFC7CFB95" box="[903,952,1058,1084]" italics="true" pageId="7" pageNumber="134">et al</emphasis>
. (2021)
</bibRefCitation>
,
<bibRefCitation id="EFA74B79FFEDFFBD35631B7AFA63FB95" author="Maharachchikumbura, S. S. &amp; Chen, Y. &amp; Ariyawansa, H. A. &amp; Hyde, K. D. &amp; Haelewaters, D. &amp; Perera, R. H. &amp; Samarakoon, M. C. &amp; Wanasinghe, D. N. &amp; Bustamante, D. E. &amp; Liu, J. K. &amp; Lawrence, D. P." box="[1054,1447,1058,1085]" pageId="7" pageNumber="134" pagination="155 - 179" refId="ref9530" refString="Maharachchikumbura, S. S., Chen, Y., Ariyawansa, H. A., Hyde, K. D., Haelewaters, D., Perera, R. H., Samarakoon, M. C., Wanasinghe, D. N., Bustamante, D. E., Liu, J. K. &amp; Lawrence, D. P. (2021) Integrative approaches for species delimitation in Ascomycota. Fungal Diversity 109 (1): 155 - 179. https: // doi. org / 10.1007 / s 13225 - 021 - 00486 - 6" type="journal article" year="2021">
Maharachchikumbura
<emphasis id="B942EA9AFFEDFFBD34661B7BFA88FB95" box="[1307,1356,1058,1084]" italics="true" pageId="7" pageNumber="134">et al</emphasis>
. (2021)
</bibRefCitation>
, and
<bibRefCitation id="EFA74B79FFEDFFBD31CA1B1FFE04FBC9" author="Jeewon, R. &amp; Hyde, K. D." box="[183,448,1094,1121]" pageId="7" pageNumber="134" pagination="1669 - 77" refId="ref8460" refString="Jeewon, R. &amp; Hyde, K. D. (2016) Establishing species boundaries and new taxa among fungi: recommendations to resolve taxonomic ambiguities. Mycosp 7 (11): 1669 - 77. https: // doi. org / 10.5943 / mycosphere / 7 / 11 / 4" type="journal article" year="2016">Jeewon &amp; Hyde (2016)</bibRefCitation>
,
<taxonomicName id="4C364D0BFFEDFFBD30B11B1FFDA9FBC9" box="[460,621,1095,1120]" class="Dothideomycetes" family="Tubeufiaceae" genus="Neomanoharachariella" higherTaxonomySource="CoL,GBIF" kingdom="Fungi" order="Tubeufiales" pageId="7" pageNumber="134" phylum="Ascomycota" rank="species" species="xizangensis">
<emphasis id="B942EA9AFFEDFFBD30B11B1FFDA9FBC9" box="[460,621,1095,1120]" italics="true" pageId="7" pageNumber="134">N. xizangensis</emphasis>
</taxonomicName>
is established as a new species.
</paragraph>
</subSubSection>
</treatment>
</document>