308 lines
56 KiB
XML
308 lines
56 KiB
XML
<document id="3A5C2F7AF9118BFFFA670A6B3E48FEB8" ID-CLB-Dataset="58894" ID-DOI="10.5852/ejt.2023.861.2063" ID-GBIF-Dataset="98f15b31-8fcd-4ea4-9621-6bff596e64fe" ID-ISSN="2118-9773" ID-Zenodo-Dep="7710258" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.taxonomicNames_approvedBy="felipe" checkinTime="1678329856084" checkinUser="felipe" docAuthor="Véron, Benoît, Rougier, Etienne, Taylor, Anthony & Goux, Didier" docDate="2023" docId="03FBF1168465FFEBFDC9F9196B73FA0F" docLanguage="en" docName="ejt-861_21-47.pdf" docOrigin="European Journal of Taxonomy 861" docStyle="DocumentStyle:EF2B578F1D15862ADE45B0C07C620911.14:EJT.2018-.journal_article.type1" docStyleId="EF2B578F1D15862ADE45B0C07C620911" docStyleName="EJT.2018-.journal_article.type1" docStyleVersion="14" docTitle="Rebecca billardiae Veron 2023, sp. nov." docType="treatment" docVersion="6" lastPageNumber="35" masterDocId="FFC2896E846FFFE5FF98FFD16A26FF91" masterDocTitle="New species of Pavlovophyceae (Haptophyta) and revision of the genera Exanthemachrysis, Rebecca and Pavlova" masterLastPageNumber="47" masterPageNumber="21" pageNumber="31" updateTime="1699476326315" updateUser="ExternalLinkService" zenodo-license-document="CC-BY-4.0" zenodo-license-figures="CC-BY-4.0" zenodo-license-treatments="UNSPECIFIED">
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<mods:title id="3A94B00761B470A65BE69C285C29622C">New species of Pavlovophyceae (Haptophyta) and revision of the genera Exanthemachrysis, Rebecca and Pavlova</mods:title>
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<mods:name id="C41B7628D6C1F6501D90480C412358B3" type="personal">
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<mods:namePart id="D59744D6B1F459B545D64CBF6125A4A5">Véron, Benoît</mods:namePart>
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<mods:affiliation id="ADD41439A52E2C93E04495518025C2C0">Laboratoire de Biologie des Organismes et Écosystèmes Aquatiques (BOREA) Université de Caen-Normandie, MNHN, SU, UA, CNRS, IRD, 14000 Caen, France. & Normandie Université, UNICAEN, UMR 8067 BOREA, MNHN, SU, CNRS, IRD- 207, UA, 14000 Caen, France.</mods:affiliation>
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<mods:nameIdentifier id="549990B7DD364B2960A5C4ABEA4AC546" type="email">benoit.veron@unicaen.fr</mods:nameIdentifier>
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<mods:namePart id="83C81BC130BDA2963AFBB8A50C3BE469">Rougier, Etienne</mods:namePart>
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<mods:affiliation id="D7372873D48950968C3EE92D6C6400AD">Laboratoire de Biologie des Organismes et Écosystèmes Aquatiques (BOREA) Université de Caen-Normandie, MNHN, SU, UA, CNRS, IRD, 14000 Caen, France. & Normandie Université, UNICAEN, UMR 8067 BOREA, MNHN, SU, CNRS, IRD- 207, UA, 14000 Caen, France.</mods:affiliation>
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<mods:nameIdentifier id="CB205E6CAA5A5EF240A2B3AEC2B144C2" type="email">etienne.rougier@gmail.com</mods:nameIdentifier>
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<mods:namePart id="600165AA7ABF511C623C6D2E83C20A36">Taylor, Anthony</mods:namePart>
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<mods:affiliation id="832F13979E8E54C270C026C8B9ABE558">Laboratoire de Biologie des Organismes et Écosystèmes Aquatiques (BOREA) Université de Caen-Normandie, MNHN, SU, UA, CNRS, IRD, 14000 Caen, France. & Normandie Université, UNICAEN, UMR 8067 BOREA, MNHN, SU, CNRS, IRD- 207, UA, 14000 Caen, France. & Institute of Marine Sciences, University of Portsmouth, Portsmouth, Hampshire, UK.</mods:affiliation>
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<mods:nameIdentifier id="C8D7B988A96BE5830E036400F83F678F" type="email">anthony.taylor2590@gmail.com</mods:nameIdentifier>
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<mods:namePart id="AD270ADAFCCF82F4F91F5458E328D5EB">Goux, Didier</mods:namePart>
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<mods:affiliation id="DEEED3BCE504D1EC34C40F63FD04E98E">Normandie Université, UNICAEN, CMAbio 3 (Centre de Microscopie Appliquée à la Biologie), SF EMerode, 14000 Caen, France. & Normandie Université, ENSICAEN, UNICAEN, CNRS, UMR 6508 CRISMAT, 14000 Caen, France.</mods:affiliation>
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<mods:nameIdentifier id="AF1CCEBAC79B9B92C8BB45BB7B523F34" type="email">didier.goux@unicaen.fr</mods:nameIdentifier>
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<mods:typeOfResource id="03951B431BD35F23A147D7BBD6060133">text</mods:typeOfResource>
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<mods:title id="B044C1F6416063FC03AE0092381A04AC">European Journal of Taxonomy</mods:title>
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<mods:part id="0D34497EB6EC90A0E6ECDB6D4301BA59">
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<mods:date id="76C4C0ED78978E99F69461BCDBCF93C4">2023</mods:date>
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<mods:number id="235C606B28778248857A218166AA53EF">2023-03-08</mods:number>
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<mods:detail id="D05064F4B751A113D32B765E5A807DBA" type="volume">
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<mods:number id="74A2D6609927991A25597C2A4D2BB594">861</mods:number>
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<mods:classification id="783435824CE63E78B86D540E1EC03F79">journal article</mods:classification>
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<mods:identifier id="53A760D56881C659009E1C6D75611F6A" type="CLB-Dataset">58894</mods:identifier>
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<mods:identifier id="0B741DD894EF0F56A99C8730B3DEA3ED" type="DOI">10.5852/ejt.2023.861.2063</mods:identifier>
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<mods:identifier id="A92AB21794C66F21809DB93674DFA870" type="GBIF-Dataset">98f15b31-8fcd-4ea4-9621-6bff596e64fe</mods:identifier>
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<mods:identifier id="A50F963BD870E6268B9D0AC36F57B478" type="ISSN">2118-9773</mods:identifier>
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<treatment id="03FBF1168465FFEBFDC9F9196B73FA0F" ID-DOI="http://doi.org/10.5281/zenodo.7713652" ID-GBIF-Taxon="209434625" ID-Zenodo-Dep="7713652" LSID="urn:lsid:plazi:treatment:03FBF1168465FFEBFDC9F9196B73FA0F" httpUri="http://treatment.plazi.org/id/03FBF1168465FFEBFDC9F9196B73FA0F" lastPageId="14" lastPageNumber="35" pageId="10" pageNumber="31">
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<subSubSection id="C348138B8465FFEFFDC9F91969B7F896" pageId="10" pageNumber="31" type="nomenclature">
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<paragraph id="8BED40008465FFEFFDC9F91969C5F975" blockId="10.[593,995,1736,1836]" box="[593,995,1736,1764]" pageId="10" pageNumber="31">
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<heading id="D0A5F76C8465FFEFFDC9F91969C5F975" box="[593,995,1736,1764]" centered="true" fontSize="11" level="2" pageId="10" pageNumber="31" reason="2">
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<taxonomicName id="4C523B838465FFEFFDC9F91969A7F972" authority="Veron" authorityName="Veron" authorityYear="2023" box="[593,897,1736,1763]" class="Pavlovophyceae" family="Pavlovaceae" genus="Rebecca" kingdom="Chromista" order="Pavlovales" pageId="10" pageNumber="31" phylum="Haptophyta" rank="species" species="billardiae" status="sp. nov.">
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<emphasis id="B9269C128465FFEFFDC9F9196917F972" bold="true" box="[593,817,1736,1763]" italics="true" pageId="10" pageNumber="31">Rebecca billardiae</emphasis>
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Véron
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</taxonomicName>
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<taxonomicNameLabel id="A21521698465FFEFFC10F91B69C5F975" box="[904,995,1738,1764]" pageId="10" pageNumber="31" rank="species">sp. nov.</taxonomicNameLabel>
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</heading>
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</paragraph>
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<paragraph id="8BED40008465FFEFFD3AF93F69B7F896" blockId="10.[593,995,1736,1836]" box="[674,913,1773,1800]" pageId="10" pageNumber="31">PhycoBank: 103627</paragraph>
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</subSubSection>
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<subSubSection id="C348138B8465FFEFFD7FF8C3696BF8BD" box="[743,845,1810,1836]" pageId="10" pageNumber="31" type="description">
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<paragraph id="8BED40008465FFEFFD7FF8C3696BF8BD" blockId="10.[593,995,1736,1836]" box="[743,845,1810,1836]" pageId="10" pageNumber="31">
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<figureCitation id="13695C858465FFEFFD7FF8C3696BF8BD" box="[743,845,1810,1836]" captionStart-0="Fig" captionStart-1="Fig" captionStart-2="Fig" captionStartId-0="11.[189,232,1716,1742]" captionStartId-1="12.[189,232,1574,1600]" captionStartId-2="13.[189,232,1716,1742]" captionTargetBox-0="[199,1385,809,1678]" captionTargetBox-1="[246,1341,265,1536]" captionTargetBox-2="[250,1337,265,1679]" captionTargetId-0="figure-125@11.[199,1385,809,1678]" captionTargetId-1="figure-15@12.[246,1341,265,1536]" captionTargetId-2="figure-12@13.[250,1337,265,1679]" captionTargetPageId-0="11" captionTargetPageId-1="12" captionTargetPageId-2="13" captionText-0="Fig. 7. Rebecca billardiae Véron sp. nov. (AC537), flagellate motile and non-motile cells, LM images. A. Gathering angular non-swimming flagellate cells with an S-shaped AF (white arrows). B. Polyhedral free-swimming cell with bilobed lateral golden-brown C, S-shaped AF (white arrow) and posterior beaded filipodium (black arrows). C–D. Cells of various shapes showing sub-apical insertion into a pit (white arrows) of the S-shaped AF. Posterior branched beaded filipodia present at the terminal part of the cell (black arrows). E. Ventral view of an angular free-swimming cell with sub-apical insertion of S-shaped AF into a pit (white arrow). F. Rounded cells with S-shaped sub-apically inserted AF (white arrow), posterior filipodium (black arrow) and bilobed golden-brown C. Scale bars: Scale bars: A = 4 μm; B–D, F = 5 μm; E = 1 μm." captionText-1="Fig. 8. Rebecca billardiae Véron sp. nov. (AC537), flagella, plasma membrane and knob-scales, TEM images (A–D, G–H = Fix 1; E–F = Fix 2). A. Angular cell with bilobed C and TH lamellae grouped by 3 sometimes twisted. B. Longitudinal section of a motile cell displaying the AF bearing few KS (white arrows) and the adjacent H. C. Cross-section of the sub-apical pit of a non-swimming cell containing the bases of H and F (transverse section – right, oblique section – left) sheathed by cytoplasm and surrounded by the plasma membrane (black arrow). D. Inflated G secretory vesicles unloading their materials including KS near the flagellar insertion zone. E. Periphery of a cell showing continuity of the plasma membrane along the curved AF, both covered with pedunculate KS (white arrows). F. Isolated plasma membrane retaining pedunculate mono-constricted club-shaped KS sporadically dispersed on its surface. G. Longitudinal section of a motile cell displaying the AF and PF both bearing few KS (white arrows) and the naked H. Note discharge of KS by G cisternae. H. Reduced PF cut in median longitudinal section showing microtubules and bearing a dense pedunculate club-shaped KS (white arrow). Central pair of microtubules is absent. Scale bars: A = 2 µm; B–D, H = 0.5 µm; E–F = 50 nm; G = 1 µm." captionText-2="Fig. 9. Rebecca billardiae Véron sp. nov. (AC537), flagellar apparatus details of motile cells. A–E. SEM images. A. Ventral view of a cuboid cell with the S-shaped distally tapered AF and curved H. B-C. Ventral views of angular cells with the S-shaped AF covered with KS, the vestigial PF and the curved bipartite H between both F. D-E. Details of the insertion the 3 appendages. D. Vestigial PF. E. Bipartite H with the proximal hook-shaped half and the distal half forming a pearl string structure. Note distally tapered AF. F–G. Negative staining images. F. Proximal part of the tomentose AF with multilayered KS (white arrow), extending after a bare proximal zone (black arrow) in the immediate vicinity of the cell. G. Details of mono-constricted (white arrows) KS covering the distally attenuated AF. Scale bars: A, C, E–F = 1 µm; B = 2 µm; D = 100 nm; G = 200 nm." figureDoi-0="http://doi.org/10.5281/zenodo.7710272" figureDoi-1="http://doi.org/10.5281/zenodo.7710274" figureDoi-2="http://doi.org/10.5281/zenodo.7710276" httpUri-0="https://zenodo.org/record/7710272/files/figure.png" httpUri-1="https://zenodo.org/record/7710274/files/figure.png" httpUri-2="https://zenodo.org/record/7710276/files/figure.png" pageId="10" pageNumber="31">Figs 7–9</figureCitation>
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</paragraph>
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</subSubSection>
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<subSubSection id="C348138B8465FFEFFF25F88468BDF878" pageId="10" pageNumber="31" type="diagnosis">
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<paragraph id="8BED40008465FFEFFF25F8846B10F8FE" blockId="10.[189,310,1877,1903]" box="[189,310,1877,1903]" pageId="10" pageNumber="31">
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<heading id="D0A5F76C8465FFEFFF25F8846B10F8FE" bold="true" box="[189,310,1877,1903]" fontSize="11" level="3" pageId="10" pageNumber="31" reason="3">
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<emphasis id="B9269C128465FFEFFF25F8846B10F8FE" bold="true" box="[189,310,1877,1903]" pageId="10" pageNumber="31">Diagnosis</emphasis>
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</heading>
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</paragraph>
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<paragraph id="8BED40008465FFEFFF25F85768BDF878" blockId="10.[189,1399,1925,2025]" pageId="10" pageNumber="31">
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In addition to the very pronounced angular or even cubic and non-metabolic shape that the new species can take,
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<taxonomicName id="4C523B838465FFEFFEA9F87B6835F855" authority="Véron & Rougier & Taylor & Goux, 2023" authorityName="Véron & Rougier & Taylor & Goux" authorityYear="2023" box="[305,531,1962,1988]" class="Pavlovophyceae" family="Pavlovaceae" genus="Rebecca" kingdom="Chromista" order="Pavlovales" pageId="10" pageNumber="31" phylum="Haptophyta" rank="species" species="billardiae" status="sp. nov.">
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<emphasis id="B9269C128465FFEFFEA9F87B6835F855" box="[305,531,1962,1988]" italics="true" pageId="10" pageNumber="31">Rebecca billardiae</emphasis>
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</taxonomicName>
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<taxonomicNameLabel id="A21521698465FFEFFD84F87B685FF855" box="[540,633,1962,1988]" pageId="10" pageNumber="31" rank="species">sp. nov.</taxonomicNameLabel>
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is also distinguished by knob-scales with only one constriction instead of two, and a slightly shorter AF.
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</paragraph>
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</subSubSection>
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<subSubSection id="C348138B8464FFEEFF25FED86EBDFE03" pageId="11" pageNumber="32" type="etymology">
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<paragraph id="8BED40008464FFEEFF25FED86B62FEB2" blockId="11.[189,324,265,291]" box="[189,324,265,291]" pageId="11" pageNumber="32">
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<heading id="D0A5F76C8464FFEEFF25FED86B62FEB2" bold="true" box="[189,324,265,291]" fontSize="11" level="3" pageId="11" pageNumber="32" reason="3">
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<emphasis id="B9269C128464FFEEFF25FED86B62FEB2" bold="true" box="[189,324,265,291]" pageId="11" pageNumber="32">Etymology</emphasis>
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</heading>
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</paragraph>
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<paragraph id="8BED40008464FFEEFF25FEE46EBDFE03" blockId="11.[189,1399,309,402]" pageId="11" pageNumber="32">
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The epithet given to the species is dedicated in honour of Professor Chantal Billard who, from the 70s to the 2010s, worked at the Université de Caen
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<collectingRegion id="49968EE28464FFEEFD7FFE876949FEE1" box="[743,879,342,368]" country="France" name="Haute-Normandie" pageId="11" pageNumber="32">Normandie</collectingRegion>
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. Chantal Billard described 29 taxa including many haptophytes and she notably described
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<taxonomicName id="4C523B838464FFEEFD4AFEA86EBDFE03" authority="(Billard) Bendif & Veron." authorityName="Bendif & Veron." baseAuthorityName="Billard" box="[722,1179,376,402]" class="Pavlovophyceae" family="Pavlovaceae" genus="Diacronema" kingdom="Chromista" order="Pavlovales" pageId="11" pageNumber="32" phylum="Haptophyta" rank="species" species="virescens">
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<emphasis id="B9269C128464FFEEFD4AFEA86944FE03" box="[722,866,377,402]" italics="true" pageId="11" pageNumber="32">D. virescens</emphasis>
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(Billard) Bendif & Véron.
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</taxonomicName>
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</paragraph>
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</subSubSection>
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<subSubSection id="C348138B8464FFEEFF25FE696B2AFDF3" pageId="11" pageNumber="32" type="materials_examined">
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<paragraph id="8BED40008464FFEEFF25FE696B4AFE40" blockId="11.[189,364,439,466]" box="[189,364,439,466]" pageId="11" pageNumber="32">
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<heading id="D0A5F76C8464FFEEFF25FE696B4AFE40" bold="true" box="[189,364,439,466]" fontSize="11" level="3" pageId="11" pageNumber="32" reason="3">
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<emphasis id="B9269C128464FFEEFF25FE696B4AFE40" bold="true" box="[189,364,439,466]" pageId="11" pageNumber="32">Type material</emphasis>
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</heading>
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</paragraph>
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<paragraph id="8BED40008464FFEEFF72FE356B2AFDF3" blockId="11.[189,1399,484,610]" pageId="11" pageNumber="32">
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<materialsCitation id="3B3A4A5D8464FFEEFF72FE356B2AFDF3" ID-GBIF-Occurrence="4052628302" collectingDate="2002-02-16" collectionCode="CN" collectorName="Fabien Jouenne" country="France" latitude="49.35" location="Baie des Veys" longLatPrecision="1104" longitude="-1.1666666" municipality="Baie des Veys" pageId="11" pageNumber="32" specimenCode="AC537" specimenCount="1" stateProvince="Normandy" typeStatus="holotype">
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<emphasis id="B9269C128464FFEEFF72FE356B7AFE6F" bold="true" box="[234,348,484,510]" pageId="11" pageNumber="32">
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<typeStatus id="54E9FEA28464FFEEFF72FE356B7AFE6F" box="[234,348,484,510]" pageId="11" pageNumber="32" type="holotype">Holotype</typeStatus>
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</emphasis>
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<collectingCountry id="F34500908464FFEEFF25FDD76B17FDB1" box="[189,305,518,544]" name="France" pageId="11" pageNumber="32">FRANCE</collectingCountry>
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•
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<collectingRegion id="49968EE28464FFEEFED7FDD46BE8FD8E" box="[335,462,517,543]" country="France" name="Haute-Normandie" pageId="11" pageNumber="32">Normandy</collectingRegion>
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,
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<collectingMunicipality id="6B89DA7A8464FFEEFE41FDD46859FDB1" box="[473,639,517,544]" pageId="11" pageNumber="32">Baie des Veys</collectingMunicipality>
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,
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<locationDeviation id="9A5F59228464FFEEFD12FDD469EEFDB1" box="[650,968,517,544]" pageId="11" pageNumber="32">Canal de Carentan à la mer</locationDeviation>
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;
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,
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<geoCoordinate id="EE6626C78464FFEEFBD3FDD56E8BFDB1" box="[1099,1197,516,544]" degrees="1" direction="west" minutes="10" orientation="longitude" pageId="11" pageNumber="32" precision="925" value="-1.1666666">1°10′ W</geoCoordinate>
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; alt. 0 m;
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<date id="FFEC66C08464FFEEFA87FDD46ADCFDD0" pageId="11" pageNumber="32" value="2002-02-16">
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<collectingDate id="EFA89F288464FFEEFA87FDD46ADCFDD0" pageId="11" pageNumber="32" value="2002-02-16">16 Feb. 2002</collectingDate>
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</date>
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;
|
||
<collectorName id="26A725D68464FFEEFE9CFDF66B90FDD0" box="[260,438,551,577]" pageId="11" pageNumber="32">Fabien Jouenne</collectorName>
|
||
leg.; Jacqueline Fresnel isol.; AC537; GenBank nos:
|
||
<accessionNumber id="9401DDE38464FFEEFB8AFDF66EA1FDD3" box="[1042,1159,551,578]" pageId="11" pageNumber="32">JF714245</accessionNumber>
|
||
,
|
||
<accessionNumber id="9401DDE38464FFEEFB08FDF66F23FDD0" box="[1168,1285,551,577]" pageId="11" pageNumber="32">JF718773</accessionNumber>
|
||
;
|
||
<accessionNumber id="9401DDE38464FFEEFA97FDF66B2EFDF3" pageId="11" pageNumber="32">CN[537- 1NM]</accessionNumber>
|
||
.
|
||
</materialsCitation>
|
||
</paragraph>
|
||
</subSubSection>
|
||
<subSubSection id="C348138B8464FFEBFF25FD566B73FA0F" lastPageId="14" lastPageNumber="35" pageId="11" pageNumber="32" type="description">
|
||
<paragraph id="8BED40008464FFEEFF25FD566B68FD30" blockId="11.[189,334,647,673]" box="[189,334,647,673]" pageId="11" pageNumber="32">
|
||
<heading id="D0A5F76C8464FFEEFF25FD566B68FD30" bold="true" box="[189,334,647,673]" fontSize="11" level="3" pageId="11" pageNumber="32" reason="3">
|
||
<emphasis id="B9269C128464FFEEFF25FD566B68FD30" bold="true" box="[189,334,647,673]" pageId="11" pageNumber="32">Description</emphasis>
|
||
</heading>
|
||
</paragraph>
|
||
<paragraph id="8BED40008464FFEBFF25FD656BBEFE45" blockId="11.[189,1399,692,752]" lastBlockId="14.[189,1400,265,468]" lastPageId="14" lastPageNumber="35" pageId="11" pageNumber="32">Motile cells not phototactic often ovate, some with strongly marked angular, even cubic forms. Less angular cells highly metabolic. Cell surface irregularly covered with stalked club-shaped knob-scales with a single constriction. Single golden-brown bilobed chloroplast with parallel and helicoidal stacks of three thylakoids; pyrenoid and eyespot absent. Posterior filipodia branched and beaded. Sinusoidal anterior flagellum covered with a tomentum of knob-scales, proximal part remaining naked. Reduced posterior flagellum bearing few knob-scales. Naked bipartite haptonema with a proximal part of constant diameter and a string of pearl-like structures on distal part. Non-swimming cells gather with twisting anterior flagellum.</paragraph>
|
||
<caption id="DF2D10888464FFEEFF25F9656B8BF879" ID-DOI="http://doi.org/10.5281/zenodo.7710272" ID-Zenodo-Dep="7710272" httpUri="https://zenodo.org/record/7710272/files/figure.png" pageId="11" pageNumber="32" startId="11.[189,232,1716,1742]" targetBox="[199,1385,809,1678]" targetPageId="11">
|
||
<paragraph id="8BED40008464FFEEFF25F9656B8BF879" blockId="11.[189,1399,1716,2024]" pageId="11" pageNumber="32">
|
||
<emphasis id="B9269C128464FFEEFF25F9656B2DF95F" bold="true" box="[189,267,1716,1742]" pageId="11" pageNumber="32">Fig. 7.</emphasis>
|
||
<taxonomicName id="4C523B838464FFEEFE8BF9656866F95F" authority="Veron" authorityName="Veron" authorityYear="2023" box="[275,576,1716,1742]" class="Pavlovophyceae" family="Pavlovaceae" genus="Rebecca" kingdom="Chromista" order="Pavlovales" pageId="11" pageNumber="32" phylum="Haptophyta" rank="species" species="billardiae" status="sp. nov.">
|
||
<emphasis id="B9269C128464FFEEFE8BF9656BD7F95F" box="[275,497,1716,1742]" italics="true" pageId="11" pageNumber="32">Rebecca billardiae</emphasis>
|
||
Véron
|
||
</taxonomicName>
|
||
<taxonomicNameLabel id="A21521698464FFEEFDD0F9646882F95E" box="[584,676,1717,1743]" pageId="11" pageNumber="32" rank="species">sp. nov.</taxonomicNameLabel>
|
||
(AC537), flagellate motile and non-motile cells, LM images.
|
||
<emphasis id="B9269C128464FFEEFF25F9066AF4F960" bold="true" box="[189,210,1751,1777]" pageId="11" pageNumber="32">A</emphasis>
|
||
. Gathering angular non-swimming flagellate cells with an S-shaped AF (white arrows).
|
||
<emphasis id="B9269C128464FFEEFB4DF9066ECFF960" bold="true" box="[1237,1257,1751,1777]" pageId="11" pageNumber="32">B</emphasis>
|
||
. Polyhedral free-swimming cell with bilobed lateral golden-brown C, S-shaped AF (white arrow) and posterior beaded filipodium (black arrows).
|
||
<emphasis id="B9269C128464FFEEFE62F8CC6815F8A6" bold="true" box="[506,563,1821,1847]" pageId="11" pageNumber="32">C–D</emphasis>
|
||
. Cells of various shapes showing sub-apical insertion into a pit (white arrows) of the S-shaped AF. Posterior branched beaded filipodia present at the terminal part of the cell (black arrows).
|
||
<emphasis id="B9269C128464FFEEFEEDF8B56BAFF8EF" bold="true" box="[373,393,1892,1918]" pageId="11" pageNumber="32">E</emphasis>
|
||
. Ventral view of an angular free-swimming cell with sub-apical insertion of S-shaped AF into a pit (white arrow).
|
||
<emphasis id="B9269C128464FFEEFD8DF8566801F830" bold="true" box="[533,551,1927,1953]" pageId="11" pageNumber="32">F</emphasis>
|
||
. Rounded cells with S-shaped sub-apically inserted AF (white arrow), posterior filipodium (black arrow) and bilobed golden-brown C. Scale bars: Scale bars: A = 4 μm; B–D, F = 5 μm; E = 1 μm.
|
||
</paragraph>
|
||
</caption>
|
||
<caption id="DF2D10888463FFE9FF25F9F76F0BF879" ID-DOI="http://doi.org/10.5281/zenodo.7710274" ID-Zenodo-Dep="7710274" httpUri="https://zenodo.org/record/7710274/files/figure.png" pageId="12" pageNumber="33" startId="12.[189,232,1574,1600]" targetBox="[246,1341,265,1536]" targetPageId="12">
|
||
<paragraph id="8BED40008463FFE9FF25F9F76F0BF879" blockId="12.[189,1400,1574,2024]" pageId="12" pageNumber="33">
|
||
<emphasis id="B9269C128463FFE9FF25F9F76B2DF9D0" bold="true" box="[189,267,1574,1601]" pageId="12" pageNumber="33">Fig. 8.</emphasis>
|
||
<taxonomicName id="4C523B838463FFE9FE8CF9F66865F9D0" authority="Veron" authorityName="Veron" authorityYear="2023" box="[276,579,1575,1601]" class="Pavlovophyceae" family="Pavlovaceae" genus="Rebecca" kingdom="Chromista" order="Pavlovales" pageId="12" pageNumber="33" phylum="Haptophyta" rank="species" species="billardiae" status="sp. nov.">
|
||
<emphasis id="B9269C128463FFE9FE8CF9F66BD4F9D0" box="[276,498,1575,1601]" italics="true" pageId="12" pageNumber="33">Rebecca billardiae</emphasis>
|
||
Véron
|
||
</taxonomicName>
|
||
<taxonomicNameLabel id="A21521698463FFE9FDD3F9F66881F9D0" box="[587,679,1575,1601]" pageId="12" pageNumber="33" rank="species">sp. nov.</taxonomicNameLabel>
|
||
(AC537), flagella, plasma membrane and knob-scales, TEM images (A–D, G–H = Fix 1; E–F = Fix 2).
|
||
<emphasis id="B9269C128463FFE9FD2DF99B68ECF9F5" bold="true" box="[693,714,1610,1636]" pageId="12" pageNumber="33">A</emphasis>
|
||
. Angular cell with bilobed C and TH lamellae grouped by 3 sometimes twisted.
|
||
<emphasis id="B9269C128463FFE9FE25F9BC6BF6F916" bold="true" box="[445,464,1645,1671]" pageId="12" pageNumber="33">B</emphasis>
|
||
. Longitudinal section of a motile cell displaying the AF bearing few KS (white arrows) and the adjacent H.
|
||
<emphasis id="B9269C128463FFE9FD88F9416803F93B" bold="true" box="[528,549,1680,1706]" pageId="12" pageNumber="33">C</emphasis>
|
||
. Cross-section of the sub-apical pit of a non-swimming cell containing the bases of H and F (transverse section – right, oblique section – left) sheathed by cytoplasm and surrounded by the plasma membrane (black arrow).
|
||
<emphasis id="B9269C128463FFE9FCA2F9066968F960" bold="true" box="[826,846,1751,1777]" pageId="12" pageNumber="33">D</emphasis>
|
||
. Inflated G secretory vesicles unloading their materials including KS near the flagellar insertion zone.
|
||
<emphasis id="B9269C128463FFE9FCD6F92B6944F885" bold="true" box="[846,866,1786,1812]" pageId="12" pageNumber="33">E</emphasis>
|
||
. Periphery of a cell showing continuity of the plasma membrane along the curved AF, both covered with pedunculate KS (white arrows).
|
||
<emphasis id="B9269C128463FFE9FB60F8CC6F2CF8A6" bold="true" box="[1272,1290,1821,1847]" pageId="12" pageNumber="33">F</emphasis>
|
||
. Isolated plasma membrane retaining pedunculate mono-constricted club-shaped KS sporadically dispersed on its surface.
|
||
<emphasis id="B9269C128463FFE9FEB9F8B26B1EF8EC" bold="true" box="[289,312,1891,1917]" pageId="12" pageNumber="33">G</emphasis>
|
||
. Longitudinal section of a motile cell displaying the AF and PF both bearing few KS (white arrows) and the naked H. Note discharge of KS by G cisternae.
|
||
<emphasis id="B9269C128463FFE9FC0AF856698FF830" bold="true" box="[914,937,1927,1953]" pageId="12" pageNumber="33">H</emphasis>
|
||
. Reduced PF cut in median longitudinal section showing microtubules and bearing a dense pedunculate club-shaped KS (white arrow). Central pair of microtubules is absent. Scale bars: A = 2 µm; B–D, H = 0.5 µm; E–F = 50 nm; G = 1 µm.
|
||
</paragraph>
|
||
</caption>
|
||
<caption id="DF2D10888462FFE8FF25F965692AF878" ID-DOI="http://doi.org/10.5281/zenodo.7710276" ID-Zenodo-Dep="7710276" httpUri="https://zenodo.org/record/7710276/files/figure.png" pageId="13" pageNumber="34" startId="13.[189,232,1716,1742]" targetBox="[250,1337,265,1679]" targetPageId="13">
|
||
<paragraph id="8BED40008462FFE8FF25F965692AF878" blockId="13.[189,1399,1716,2025]" pageId="13" pageNumber="34">
|
||
<emphasis id="B9269C128462FFE8FF25F9656B2FF95E" bold="true" box="[189,265,1716,1743]" pageId="13" pageNumber="34">Fig. 9.</emphasis>
|
||
<taxonomicName id="4C523B838462FFE8FE97F964681FF95E" authority="Veron" authorityName="Veron" authorityYear="2023" box="[271,569,1717,1743]" class="Pavlovophyceae" family="Pavlovaceae" genus="Rebecca" kingdom="Chromista" order="Pavlovales" pageId="13" pageNumber="34" phylum="Haptophyta" rank="species" species="billardiae" status="sp. nov.">
|
||
<emphasis id="B9269C128462FFE8FE97F9646BCDF95E" box="[271,491,1717,1743]" italics="true" pageId="13" pageNumber="34">Rebecca billardiae</emphasis>
|
||
Véron
|
||
</taxonomicName>
|
||
<taxonomicNameLabel id="A21521698462FFE8FDD8F96468BFF95E" box="[576,665,1717,1743]" pageId="13" pageNumber="34" rank="species">sp. nov.</taxonomicNameLabel>
|
||
(AC537), flagellar apparatus details of motile cells.
|
||
<emphasis id="B9269C128462FFE8FB6DF9656F0BF95F" bold="true" box="[1269,1325,1716,1742]" pageId="13" pageNumber="34">A–E</emphasis>
|
||
. SEM images.
|
||
<emphasis id="B9269C128462FFE8FEBAF9096B11F963" bold="true" box="[290,311,1752,1778]" pageId="13" pageNumber="34">A</emphasis>
|
||
. Ventral view of a cuboid cell with the S-shaped distally tapered AF and curved H.
|
||
<emphasis id="B9269C128462FFE8FAA4F9096F49F963" bold="true" box="[1340,1391,1752,1778]" pageId="13" pageNumber="34">B-C</emphasis>
|
||
. Ventral views of angular cells with the S-shaped AF covered with KS, the vestigial PF and the curved bipartite H between both F.
|
||
<emphasis id="B9269C128462FFE8FD9BF8CF6810F8A9" bold="true" box="[515,566,1822,1848]" pageId="13" pageNumber="34">D-E</emphasis>
|
||
. Details of the insertion the 3 appendages.
|
||
<emphasis id="B9269C128462FFE8FBA8F8CF6E63F8A9" bold="true" box="[1072,1093,1822,1848]" pageId="13" pageNumber="34">D</emphasis>
|
||
. Vestigial PF.
|
||
<emphasis id="B9269C128462FFE8FB76F8CF6F24F8A9" bold="true" box="[1262,1282,1822,1848]" pageId="13" pageNumber="34">E</emphasis>
|
||
. Bipartite H with the proximal hook-shaped half and the distal half forming a pearl string structure. Note distally tapered AF.
|
||
<emphasis id="B9269C128462FFE8FED1F8B56BA6F8EF" bold="true" box="[329,384,1892,1918]" pageId="13" pageNumber="34">F–G</emphasis>
|
||
. Negative staining images.
|
||
<emphasis id="B9269C128462FFE8FD5AF8B468F2F8EE" bold="true" box="[706,724,1893,1919]" pageId="13" pageNumber="34">F</emphasis>
|
||
. Proximal part of the tomentose AF with multilayered KS (white arrow), extending after a bare proximal zone (black arrow) in the immediate vicinity of the cell.
|
||
<emphasis id="B9269C128462FFE8FF25F87A6AF2F854" bold="true" box="[189,212,1963,1989]" pageId="13" pageNumber="34">G</emphasis>
|
||
. Details of mono-constricted (white arrows) KS covering the distally attenuated AF. Scale bars: A, C, E–F = 1 µm; B = 2 µm; D = 100 nm; G = 200 nm.
|
||
</paragraph>
|
||
</caption>
|
||
<paragraph id="8BED40008461FFEBFF25FE2D6869FD87" blockId="14.[189,591,508,534]" box="[189,591,508,534]" pageId="14" pageNumber="35">
|
||
<heading id="D0A5F76C8461FFEBFF25FE2D6869FD87" bold="true" box="[189,591,508,534]" fontSize="11" level="3" pageId="14" pageNumber="35" reason="3">
|
||
<emphasis id="B9269C128461FFEBFF25FE2D6869FD87" bold="true" box="[189,591,508,534]" pageId="14" pageNumber="35">Microscopy and related analysis</emphasis>
|
||
</heading>
|
||
</paragraph>
|
||
<paragraph id="8BED40008461FFEBFF25FDFA6BFDFB77" blockId="14.[189,1399,555,1255]" pageId="14" pageNumber="35">
|
||
After nearly two months of culture on agar plates, no colonies developed. Apparently only the flagellate stage exists in this species and consists of freely swimming cells. Often ovate (7.5 µm ± 0.6 × 6.1 µm ± 0.6, n = 50), cells can display very variable shapes, including cubic forms but mainly cells have flat unequal sides forming acute or obtuse angles at the circumference (
|
||
<figureCitation id="13695C858461FFEBFC5DFD446E05FD3E" box="[965,1059,661,687]" captionStart="Fig" captionStartId="11.[189,232,1716,1742]" captionTargetBox="[199,1385,809,1678]" captionTargetId="figure-125@11.[199,1385,809,1678]" captionTargetPageId="11" captionText="Fig. 7. Rebecca billardiae Véron sp. nov. (AC537), flagellate motile and non-motile cells, LM images. A. Gathering angular non-swimming flagellate cells with an S-shaped AF (white arrows). B. Polyhedral free-swimming cell with bilobed lateral golden-brown C, S-shaped AF (white arrow) and posterior beaded filipodium (black arrows). C–D. Cells of various shapes showing sub-apical insertion into a pit (white arrows) of the S-shaped AF. Posterior branched beaded filipodia present at the terminal part of the cell (black arrows). E. Ventral view of an angular free-swimming cell with sub-apical insertion of S-shaped AF into a pit (white arrow). F. Rounded cells with S-shaped sub-apically inserted AF (white arrow), posterior filipodium (black arrow) and bilobed golden-brown C. Scale bars: Scale bars: A = 4 μm; B–D, F = 5 μm; E = 1 μm." figureDoi="http://doi.org/10.5281/zenodo.7710272" httpUri="https://zenodo.org/record/7710272/files/figure.png" pageId="14" pageNumber="35">Fig. 7A</figureCitation>
|
||
). When cells stop swimming, they gather, seeming to nestle together as closely as possible while keeping their long flagella beating (
|
||
<figureCitation id="13695C858461FFEBFF5EFD0D6B03FD67" box="[198,293,732,758]" captionStart="Fig" captionStartId="11.[189,232,1716,1742]" captionTargetBox="[199,1385,809,1678]" captionTargetId="figure-125@11.[199,1385,809,1678]" captionTargetPageId="11" captionText="Fig. 7. Rebecca billardiae Véron sp. nov. (AC537), flagellate motile and non-motile cells, LM images. A. Gathering angular non-swimming flagellate cells with an S-shaped AF (white arrows). B. Polyhedral free-swimming cell with bilobed lateral golden-brown C, S-shaped AF (white arrow) and posterior beaded filipodium (black arrows). C–D. Cells of various shapes showing sub-apical insertion into a pit (white arrows) of the S-shaped AF. Posterior branched beaded filipodia present at the terminal part of the cell (black arrows). E. Ventral view of an angular free-swimming cell with sub-apical insertion of S-shaped AF into a pit (white arrow). F. Rounded cells with S-shaped sub-apically inserted AF (white arrow), posterior filipodium (black arrow) and bilobed golden-brown C. Scale bars: Scale bars: A = 4 μm; B–D, F = 5 μm; E = 1 μm." figureDoi="http://doi.org/10.5281/zenodo.7710272" httpUri="https://zenodo.org/record/7710272/files/figure.png" pageId="14" pageNumber="35">Fig. 7A</figureCitation>
|
||
). The appendages emerge from a sub-apical ventral pit (
|
||
<figureCitation id="13695C858461FFEBFC2FFD0D6E33FD66" box="[951,1045,732,759]" captionStart="Fig" captionStartId="11.[189,232,1716,1742]" captionTargetBox="[199,1385,809,1678]" captionTargetId="figure-125@11.[199,1385,809,1678]" captionTargetPageId="11" captionText="Fig. 7. Rebecca billardiae Véron sp. nov. (AC537), flagellate motile and non-motile cells, LM images. A. Gathering angular non-swimming flagellate cells with an S-shaped AF (white arrows). B. Polyhedral free-swimming cell with bilobed lateral golden-brown C, S-shaped AF (white arrow) and posterior beaded filipodium (black arrows). C–D. Cells of various shapes showing sub-apical insertion into a pit (white arrows) of the S-shaped AF. Posterior branched beaded filipodia present at the terminal part of the cell (black arrows). E. Ventral view of an angular free-swimming cell with sub-apical insertion of S-shaped AF into a pit (white arrow). F. Rounded cells with S-shaped sub-apically inserted AF (white arrow), posterior filipodium (black arrow) and bilobed golden-brown C. Scale bars: Scale bars: A = 4 μm; B–D, F = 5 μm; E = 1 μm." figureDoi="http://doi.org/10.5281/zenodo.7710272" httpUri="https://zenodo.org/record/7710272/files/figure.png" pageId="14" pageNumber="35">Figs 7E</figureCitation>
|
||
,
|
||
<figureCitation id="13695C858461FFEBFBBAFD0D6E4EFD67" box="[1058,1128,732,758]" captionStart="Fig" captionStartId="13.[189,232,1716,1742]" captionTargetBox="[250,1337,265,1679]" captionTargetId="figure-12@13.[250,1337,265,1679]" captionTargetPageId="13" captionText="Fig. 9. Rebecca billardiae Véron sp. nov. (AC537), flagellar apparatus details of motile cells. A–E. SEM images. A. Ventral view of a cuboid cell with the S-shaped distally tapered AF and curved H. B-C. Ventral views of angular cells with the S-shaped AF covered with KS, the vestigial PF and the curved bipartite H between both F. D-E. Details of the insertion the 3 appendages. D. Vestigial PF. E. Bipartite H with the proximal hook-shaped half and the distal half forming a pearl string structure. Note distally tapered AF. F–G. Negative staining images. F. Proximal part of the tomentose AF with multilayered KS (white arrow), extending after a bare proximal zone (black arrow) in the immediate vicinity of the cell. G. Details of mono-constricted (white arrows) KS covering the distally attenuated AF. Scale bars: A, C, E–F = 1 µm; B = 2 µm; D = 100 nm; G = 200 nm." figureDoi="http://doi.org/10.5281/zenodo.7710276" httpUri="https://zenodo.org/record/7710276/files/figure.png" pageId="14" pageNumber="35">9A–E</figureCitation>
|
||
) surrounded at its base by a membrane sheath containing cytoplasm (
|
||
<figureCitation id="13695C858461FFEBFD48FD2E690DFC8B" box="[720,811,767,794]" captionStart="Fig" captionStartId="12.[189,232,1574,1600]" captionTargetBox="[246,1341,265,1536]" captionTargetId="figure-15@12.[246,1341,265,1536]" captionTargetPageId="12" captionText="Fig. 8. Rebecca billardiae Véron sp. nov. (AC537), flagella, plasma membrane and knob-scales, TEM images (A–D, G–H = Fix 1; E–F = Fix 2). A. Angular cell with bilobed C and TH lamellae grouped by 3 sometimes twisted. B. Longitudinal section of a motile cell displaying the AF bearing few KS (white arrows) and the adjacent H. C. Cross-section of the sub-apical pit of a non-swimming cell containing the bases of H and F (transverse section – right, oblique section – left) sheathed by cytoplasm and surrounded by the plasma membrane (black arrow). D. Inflated G secretory vesicles unloading their materials including KS near the flagellar insertion zone. E. Periphery of a cell showing continuity of the plasma membrane along the curved AF, both covered with pedunculate KS (white arrows). F. Isolated plasma membrane retaining pedunculate mono-constricted club-shaped KS sporadically dispersed on its surface. G. Longitudinal section of a motile cell displaying the AF and PF both bearing few KS (white arrows) and the naked H. Note discharge of KS by G cisternae. H. Reduced PF cut in median longitudinal section showing microtubules and bearing a dense pedunculate club-shaped KS (white arrow). Central pair of microtubules is absent. Scale bars: A = 2 µm; B–D, H = 0.5 µm; E–F = 50 nm; G = 1 µm." figureDoi="http://doi.org/10.5281/zenodo.7710274" httpUri="https://zenodo.org/record/7710274/files/figure.png" pageId="14" pageNumber="35">Fig. 8C</figureCitation>
|
||
). There is a very clear difference between the three appendages present: the long wavy sinusoidal AF measures between 9 and 17 µm (13.6 µm ± 3.7, n = 17) and is covered, except at its base, by a thick fluff of mono-constricted KS (
|
||
<quantity id="4CAAEDE58461FFEBFBFEFC976E94FCF0" box="[1126,1202,838,865]" metricMagnitude="-8" metricUnit="m" metricValue="4.5" pageId="14" pageNumber="35" unit="nm" value="45.0">45 nm</quantity>
|
||
×
|
||
<quantity id="4CAAEDE58461FFEBFB4BFC976F07FCF0" box="[1235,1313,838,865]" metricMagnitude="-8" metricUnit="m" metricValue="2.2" pageId="14" pageNumber="35" unit="nm" value="22.0">22 nm</quantity>
|
||
, n = 2) (
|
||
<figureCitation id="13695C858461FFEBFF5EFCBB6B62FC15" box="[198,324,874,900]" captionStart="Fig" captionStartId="13.[189,232,1716,1742]" captionTargetBox="[250,1337,265,1679]" captionTargetId="figure-12@13.[250,1337,265,1679]" captionTargetPageId="13" captionText="Fig. 9. Rebecca billardiae Véron sp. nov. (AC537), flagellar apparatus details of motile cells. A–E. SEM images. A. Ventral view of a cuboid cell with the S-shaped distally tapered AF and curved H. B-C. Ventral views of angular cells with the S-shaped AF covered with KS, the vestigial PF and the curved bipartite H between both F. D-E. Details of the insertion the 3 appendages. D. Vestigial PF. E. Bipartite H with the proximal hook-shaped half and the distal half forming a pearl string structure. Note distally tapered AF. F–G. Negative staining images. F. Proximal part of the tomentose AF with multilayered KS (white arrow), extending after a bare proximal zone (black arrow) in the immediate vicinity of the cell. G. Details of mono-constricted (white arrows) KS covering the distally attenuated AF. Scale bars: A, C, E–F = 1 µm; B = 2 µm; D = 100 nm; G = 200 nm." figureDoi="http://doi.org/10.5281/zenodo.7710276" httpUri="https://zenodo.org/record/7710276/files/figure.png" pageId="14" pageNumber="35">Fig. 9F–G</figureCitation>
|
||
), at the tip it is tapered. The PF is atrophied forming a ≈
|
||
<quantity id="4CAAEDE58461FFEBFC72FCBB6E65FC15" box="[1002,1091,874,900]" metricMagnitude="-7" metricUnit="m" metricValue="2.0" pageId="14" pageNumber="35" unit="nm" value="200.0">200 nm</quantity>
|
||
high hump (
|
||
<figureCitation id="13695C858461FFEBFB44FCBB6F19FC15" box="[1244,1343,874,900]" captionStart="Fig" captionStartId="12.[189,232,1574,1600]" captionTargetBox="[246,1341,265,1536]" captionTargetId="figure-15@12.[246,1341,265,1536]" captionTargetPageId="12" captionText="Fig. 8. Rebecca billardiae Véron sp. nov. (AC537), flagella, plasma membrane and knob-scales, TEM images (A–D, G–H = Fix 1; E–F = Fix 2). A. Angular cell with bilobed C and TH lamellae grouped by 3 sometimes twisted. B. Longitudinal section of a motile cell displaying the AF bearing few KS (white arrows) and the adjacent H. C. Cross-section of the sub-apical pit of a non-swimming cell containing the bases of H and F (transverse section – right, oblique section – left) sheathed by cytoplasm and surrounded by the plasma membrane (black arrow). D. Inflated G secretory vesicles unloading their materials including KS near the flagellar insertion zone. E. Periphery of a cell showing continuity of the plasma membrane along the curved AF, both covered with pedunculate KS (white arrows). F. Isolated plasma membrane retaining pedunculate mono-constricted club-shaped KS sporadically dispersed on its surface. G. Longitudinal section of a motile cell displaying the AF and PF both bearing few KS (white arrows) and the naked H. Note discharge of KS by G cisternae. H. Reduced PF cut in median longitudinal section showing microtubules and bearing a dense pedunculate club-shaped KS (white arrow). Central pair of microtubules is absent. Scale bars: A = 2 µm; B–D, H = 0.5 µm; E–F = 50 nm; G = 1 µm." figureDoi="http://doi.org/10.5281/zenodo.7710274" httpUri="https://zenodo.org/record/7710274/files/figure.png" pageId="14" pageNumber="35">Figs 8H</figureCitation>
|
||
,
|
||
<figureCitation id="13695C858461FFEBFAD5FCBB6AF0FC36" captionStart="Fig" captionStartId="13.[189,232,1716,1742]" captionTargetBox="[250,1337,265,1679]" captionTargetId="figure-12@13.[250,1337,265,1679]" captionTargetPageId="13" captionText="Fig. 9. Rebecca billardiae Véron sp. nov. (AC537), flagellar apparatus details of motile cells. A–E. SEM images. A. Ventral view of a cuboid cell with the S-shaped distally tapered AF and curved H. B-C. Ventral views of angular cells with the S-shaped AF covered with KS, the vestigial PF and the curved bipartite H between both F. D-E. Details of the insertion the 3 appendages. D. Vestigial PF. E. Bipartite H with the proximal hook-shaped half and the distal half forming a pearl string structure. Note distally tapered AF. F–G. Negative staining images. F. Proximal part of the tomentose AF with multilayered KS (white arrow), extending after a bare proximal zone (black arrow) in the immediate vicinity of the cell. G. Details of mono-constricted (white arrows) KS covering the distally attenuated AF. Scale bars: A, C, E–F = 1 µm; B = 2 µm; D = 100 nm; G = 200 nm." figureDoi="http://doi.org/10.5281/zenodo.7710276" httpUri="https://zenodo.org/record/7710276/files/figure.png" pageId="14" pageNumber="35">9B, D</figureCitation>
|
||
). The H is well developed (3.0 µm ± 0.6, n = 8) and consists of two sections of almost equal length, the proximal part being of constant diameter, in the shape of a twisted hook (
|
||
<figureCitation id="13695C858461FFEBFBACFC606E94FC5A" box="[1076,1202,945,971]" captionStart="Fig" captionStartId="13.[189,232,1716,1742]" captionTargetBox="[250,1337,265,1679]" captionTargetId="figure-12@13.[250,1337,265,1679]" captionTargetPageId="13" captionText="Fig. 9. Rebecca billardiae Véron sp. nov. (AC537), flagellar apparatus details of motile cells. A–E. SEM images. A. Ventral view of a cuboid cell with the S-shaped distally tapered AF and curved H. B-C. Ventral views of angular cells with the S-shaped AF covered with KS, the vestigial PF and the curved bipartite H between both F. D-E. Details of the insertion the 3 appendages. D. Vestigial PF. E. Bipartite H with the proximal hook-shaped half and the distal half forming a pearl string structure. Note distally tapered AF. F–G. Negative staining images. F. Proximal part of the tomentose AF with multilayered KS (white arrow), extending after a bare proximal zone (black arrow) in the immediate vicinity of the cell. G. Details of mono-constricted (white arrows) KS covering the distally attenuated AF. Scale bars: A, C, E–F = 1 µm; B = 2 µm; D = 100 nm; G = 200 nm." figureDoi="http://doi.org/10.5281/zenodo.7710276" httpUri="https://zenodo.org/record/7710276/files/figure.png" pageId="14" pageNumber="35">Fig. 9A–E</figureCitation>
|
||
), while the distal part, smaller in diameter, presents regular constrictions with the appearance of a pearl necklace (
|
||
<figureCitation id="13695C858461FFEBFA8FFC056AF5FB83" captionStart="Fig" captionStartId="13.[189,232,1716,1742]" captionTargetBox="[250,1337,265,1679]" captionTargetId="figure-12@13.[250,1337,265,1679]" captionTargetPageId="13" captionText="Fig. 9. Rebecca billardiae Véron sp. nov. (AC537), flagellar apparatus details of motile cells. A–E. SEM images. A. Ventral view of a cuboid cell with the S-shaped distally tapered AF and curved H. B-C. Ventral views of angular cells with the S-shaped AF covered with KS, the vestigial PF and the curved bipartite H between both F. D-E. Details of the insertion the 3 appendages. D. Vestigial PF. E. Bipartite H with the proximal hook-shaped half and the distal half forming a pearl string structure. Note distally tapered AF. F–G. Negative staining images. F. Proximal part of the tomentose AF with multilayered KS (white arrow), extending after a bare proximal zone (black arrow) in the immediate vicinity of the cell. G. Details of mono-constricted (white arrows) KS covering the distally attenuated AF. Scale bars: A, C, E–F = 1 µm; B = 2 µm; D = 100 nm; G = 200 nm." figureDoi="http://doi.org/10.5281/zenodo.7710276" httpUri="https://zenodo.org/record/7710276/files/figure.png" pageId="14" pageNumber="35">Fig. 9B, E</figureCitation>
|
||
). The two flagella as well as the cell body (
|
||
<figureCitation id="13695C858461FFEBFD41FC29695BFB83" box="[729,893,1016,1042]" captionStart="Fig" captionStartId="13.[189,232,1716,1742]" captionTargetBox="[250,1337,265,1679]" captionTargetId="figure-12@13.[250,1337,265,1679]" captionTargetPageId="13" captionText="Fig. 9. Rebecca billardiae Véron sp. nov. (AC537), flagellar apparatus details of motile cells. A–E. SEM images. A. Ventral view of a cuboid cell with the S-shaped distally tapered AF and curved H. B-C. Ventral views of angular cells with the S-shaped AF covered with KS, the vestigial PF and the curved bipartite H between both F. D-E. Details of the insertion the 3 appendages. D. Vestigial PF. E. Bipartite H with the proximal hook-shaped half and the distal half forming a pearl string structure. Note distally tapered AF. F–G. Negative staining images. F. Proximal part of the tomentose AF with multilayered KS (white arrow), extending after a bare proximal zone (black arrow) in the immediate vicinity of the cell. G. Details of mono-constricted (white arrows) KS covering the distally attenuated AF. Scale bars: A, C, E–F = 1 µm; B = 2 µm; D = 100 nm; G = 200 nm." figureDoi="http://doi.org/10.5281/zenodo.7710276" httpUri="https://zenodo.org/record/7710276/files/figure.png" pageId="14" pageNumber="35">Fig. 9C, E–G</figureCitation>
|
||
), but unlike the H, are covered with knobscales in the form of pedunculated and clavate structure with a single median constriction (
|
||
<figureCitation id="13695C858461FFEBFB6DFBCA6F54FBA4" box="[1269,1394,1051,1077]" captionStart="Fig" captionStartId="12.[189,232,1574,1600]" captionTargetBox="[246,1341,265,1536]" captionTargetId="figure-15@12.[246,1341,265,1536]" captionTargetPageId="12" captionText="Fig. 8. Rebecca billardiae Véron sp. nov. (AC537), flagella, plasma membrane and knob-scales, TEM images (A–D, G–H = Fix 1; E–F = Fix 2). A. Angular cell with bilobed C and TH lamellae grouped by 3 sometimes twisted. B. Longitudinal section of a motile cell displaying the AF bearing few KS (white arrows) and the adjacent H. C. Cross-section of the sub-apical pit of a non-swimming cell containing the bases of H and F (transverse section – right, oblique section – left) sheathed by cytoplasm and surrounded by the plasma membrane (black arrow). D. Inflated G secretory vesicles unloading their materials including KS near the flagellar insertion zone. E. Periphery of a cell showing continuity of the plasma membrane along the curved AF, both covered with pedunculate KS (white arrows). F. Isolated plasma membrane retaining pedunculate mono-constricted club-shaped KS sporadically dispersed on its surface. G. Longitudinal section of a motile cell displaying the AF and PF both bearing few KS (white arrows) and the naked H. Note discharge of KS by G cisternae. H. Reduced PF cut in median longitudinal section showing microtubules and bearing a dense pedunculate club-shaped KS (white arrow). Central pair of microtubules is absent. Scale bars: A = 2 µm; B–D, H = 0.5 µm; E–F = 50 nm; G = 1 µm." figureDoi="http://doi.org/10.5281/zenodo.7710274" httpUri="https://zenodo.org/record/7710274/files/figure.png" pageId="14" pageNumber="35">Figs 8E–F</figureCitation>
|
||
,
|
||
<figureCitation id="13695C858461FFEBFF25FBEE6AC2FBC8" box="[189,228,1087,1113]" captionStart="Fig" captionStartId="13.[189,232,1716,1742]" captionTargetBox="[250,1337,265,1679]" captionTargetId="figure-12@13.[250,1337,265,1679]" captionTargetPageId="13" captionText="Fig. 9. Rebecca billardiae Véron sp. nov. (AC537), flagellar apparatus details of motile cells. A–E. SEM images. A. Ventral view of a cuboid cell with the S-shaped distally tapered AF and curved H. B-C. Ventral views of angular cells with the S-shaped AF covered with KS, the vestigial PF and the curved bipartite H between both F. D-E. Details of the insertion the 3 appendages. D. Vestigial PF. E. Bipartite H with the proximal hook-shaped half and the distal half forming a pearl string structure. Note distally tapered AF. F–G. Negative staining images. F. Proximal part of the tomentose AF with multilayered KS (white arrow), extending after a bare proximal zone (black arrow) in the immediate vicinity of the cell. G. Details of mono-constricted (white arrows) KS covering the distally attenuated AF. Scale bars: A, C, E–F = 1 µm; B = 2 µm; D = 100 nm; G = 200 nm." figureDoi="http://doi.org/10.5281/zenodo.7710276" httpUri="https://zenodo.org/record/7710276/files/figure.png" pageId="14" pageNumber="35">9G</figureCitation>
|
||
). The scales are sporadically distributed on the plasmic membrane and on the diminutive PF while on the long AF they may form a thick tomentum of several layers of lines oriented perpendicular to the flagellum axis and forming regular rows (
|
||
<figureCitation id="13695C858461FFEBFD33FB54690DFB31" box="[683,811,1157,1184]" captionStart="Fig" captionStartId="13.[189,232,1716,1742]" captionTargetBox="[250,1337,265,1679]" captionTargetId="figure-12@13.[250,1337,265,1679]" captionTargetPageId="13" captionText="Fig. 9. Rebecca billardiae Véron sp. nov. (AC537), flagellar apparatus details of motile cells. A–E. SEM images. A. Ventral view of a cuboid cell with the S-shaped distally tapered AF and curved H. B-C. Ventral views of angular cells with the S-shaped AF covered with KS, the vestigial PF and the curved bipartite H between both F. D-E. Details of the insertion the 3 appendages. D. Vestigial PF. E. Bipartite H with the proximal hook-shaped half and the distal half forming a pearl string structure. Note distally tapered AF. F–G. Negative staining images. F. Proximal part of the tomentose AF with multilayered KS (white arrow), extending after a bare proximal zone (black arrow) in the immediate vicinity of the cell. G. Details of mono-constricted (white arrows) KS covering the distally attenuated AF. Scale bars: A, C, E–F = 1 µm; B = 2 µm; D = 100 nm; G = 200 nm." figureDoi="http://doi.org/10.5281/zenodo.7710276" httpUri="https://zenodo.org/record/7710276/files/figure.png" pageId="14" pageNumber="35">Fig. 9F–G</figureCitation>
|
||
). On their posterior end, the cells develop beaded and branched filipodia often of great length, as long as the cells or the AF, and appearing to trail behind the cells (
|
||
<figureCitation id="13695C858461FFEBFEB6FB1D6BE8FB77" box="[302,462,1228,1255]" captionStart="Fig" captionStartId="11.[189,232,1716,1742]" captionTargetBox="[199,1385,809,1678]" captionTargetId="figure-125@11.[199,1385,809,1678]" captionTargetPageId="11" captionText="Fig. 7. Rebecca billardiae Véron sp. nov. (AC537), flagellate motile and non-motile cells, LM images. A. Gathering angular non-swimming flagellate cells with an S-shaped AF (white arrows). B. Polyhedral free-swimming cell with bilobed lateral golden-brown C, S-shaped AF (white arrow) and posterior beaded filipodium (black arrows). C–D. Cells of various shapes showing sub-apical insertion into a pit (white arrows) of the S-shaped AF. Posterior branched beaded filipodia present at the terminal part of the cell (black arrows). E. Ventral view of an angular free-swimming cell with sub-apical insertion of S-shaped AF into a pit (white arrow). F. Rounded cells with S-shaped sub-apically inserted AF (white arrow), posterior filipodium (black arrow) and bilobed golden-brown C. Scale bars: Scale bars: A = 4 μm; B–D, F = 5 μm; E = 1 μm." figureDoi="http://doi.org/10.5281/zenodo.7710272" httpUri="https://zenodo.org/record/7710272/files/figure.png" pageId="14" pageNumber="35">Fig. 7B–D, F</figureCitation>
|
||
).
|
||
</paragraph>
|
||
<paragraph id="8BED40008461FFEBFF25FADF69F3FAB8" blockId="14.[189,981,1294,1321]" box="[189,981,1294,1321]" pageId="14" pageNumber="35">
|
||
<emphasis id="B9269C128461FFEBFF25FADF69F3FAB8" bold="true" box="[189,981,1294,1321]" pageId="14" pageNumber="35">
|
||
Taxonomic outcome: a revised description of the genus
|
||
<taxonomicName id="4C523B838461FFEBFCE8FADF69F3FAB8" box="[880,981,1294,1321]" class="Pavlovophyceae" family="Pavlovaceae" genus="Rebecca" kingdom="Chromista" order="Pavlovales" pageId="14" pageNumber="35" phylum="Haptophyta" rank="genus">
|
||
<emphasis id="B9269C128461FFEBFCE8FADF69F3FAB8" bold="true" box="[880,981,1294,1321]" italics="true" pageId="14" pageNumber="35">Rebecca</emphasis>
|
||
</taxonomicName>
|
||
</emphasis>
|
||
</paragraph>
|
||
<paragraph id="8BED40008461FFEBFF25FAEC6B73FA0F" blockId="14.[189,1398,1341,1438]" pageId="14" pageNumber="35">
|
||
Although retaining the basic characters of the genus
|
||
<taxonomicName id="4C523B838461FFEBFCD3FAEC6989FAC6" box="[843,943,1341,1367]" class="Pavlovophyceae" family="Pavlovaceae" genus="Rebecca" kingdom="Chromista" order="Pavlovales" pageId="14" pageNumber="35" phylum="Haptophyta" rank="genus">
|
||
<emphasis id="B9269C128461FFEBFCD3FAEC6989FAC6" box="[843,943,1341,1367]" italics="true" pageId="14" pageNumber="35">Rebecca</emphasis>
|
||
</taxonomicName>
|
||
, the species described here,
|
||
<taxonomicName id="4C523B838461FFEBFA89FAEC6BA0FAEB" authority="Veron" authorityName="Veron" authorityYear="2023" class="Pavlovophyceae" family="Pavlovaceae" genus="Rebecca" kingdom="Chromista" order="Pavlovales" pageId="14" pageNumber="35" phylum="Haptophyta" rank="species" species="billardiae" status="sp. nov.">
|
||
<emphasis id="B9269C128461FFEBFA89FAEC6B14FAEB" italics="true" pageId="14" pageNumber="35">Rebecca billardiae</emphasis>
|
||
Véron
|
||
</taxonomicName>
|
||
<taxonomicNameLabel id="A21521698461FFEBFE17FAB06BD6FAEA" box="[399,496,1377,1403]" pageId="14" pageNumber="35" rank="species">sp. nov.</taxonomicNameLabel>
|
||
, requires emendation of the description given by J.C. Green in
|
||
<bibRefCitation id="EFC33DF18461FFEBFB60FAB16B77FA0F" author="Edvardsen B. & Eikrem W. & Green J. C. & Andersen R. A. & Moon-van-der-Staay S. Y. & Medlin L. K." pageId="14" pageNumber="35" pagination="19 - 35" refId="ref12149" refString="Edvardsen B., Eikrem W., Green J. C., Andersen R. A., Moon-van-der-Staay S. Y. & Medlin L. K. 2000. Phylogenetic reconstructions of the Haptophyta inferred from 18 S ribosomal DNA sequences and available morphological data. Phycologia 39: 19 - 35. https: // doi. org / 10.2216 / i 0031 - 8884 - 39 - 1 - 19.1" type="journal article" year="2000">
|
||
Edvardsen
|
||
<emphasis id="B9269C128461FFEBFF25FA546ADEFA0F" box="[189,248,1412,1438]" italics="true" pageId="14" pageNumber="35">et al.</emphasis>
|
||
(2000)
|
||
</bibRefCitation>
|
||
.
|
||
</paragraph>
|
||
</subSubSection>
|
||
</treatment>
|
||
</document> |