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<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.90.83829" ID-GBIF-Dataset="64914430-e094-4c20-b575-df4480b989ae" ID-PMC="PMC9849089" ID-Pensoft-Pub="1314-4049-90-1" ID-Pensoft-UUID="C1715A386710534A931CFF0C383540BD" ID-PubMed="36760421" ModsDocID="1314-4049-90-1" checkinTime="1653563593057" checkinUser="pensoft" docAuthor="Chai, Chun-Yue, Gao, Wan-Li, Yan, Zhen-Li &amp; Hui, Feng-Li" docDate="2022" docId="57BF658EC9D558BC82D87077A00BF9EE" docLanguage="en" docName="MycoKeys 90: 1-18" docOrigin="MycoKeys 90" docPubDate="2022-05-25" docSource="http://dx.doi.org/10.3897/mycokeys.90.83829" docTitle="Sugiyamaella cylindrica C. Y. Chai &amp; F. L. Hui 2022, sp. nov." docType="treatment" docVersion="4" id="C1715A386710534A931CFF0C383540BD" lastPageNumber="1" masterDocId="C1715A386710534A931CFF0C383540BD" masterDocTitle="Four new species of Trichomonascaceae (Saccharomycetales, Saccharomycetes) from Central China" masterLastPageNumber="18" masterPageNumber="1" pageNumber="1" updateTime="1732480943962" updateUser="ExternalLinkService">
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<mods:title id="FDEE3806F7D67CA4F66FEA1F7A5AB3EC">Four new species of Trichomonascaceae (Saccharomycetales, Saccharomycetes) from Central China</mods:title>
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<mods:namePart id="F4F43BBFEB861FD80314563ABAF7D02F">Chai, Chun-Yue</mods:namePart>
<mods:affiliation id="3A6855BDA881CBE8FA33A0073E23DE06">School of Life Science and Agricultural Engineering, Nanyang Normal University, Nanyang 473061, China &amp; Research Center of Henan Provincial Agricultural Biomass Resource Engineering and Technology, Nanyang 473061, China</mods:affiliation>
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<mods:namePart id="FF31117B85E4B664E4B298124000E9D2">Gao, Wan-Li</mods:namePart>
<mods:affiliation id="3ECEA7E507A040685BF6B9B5FD572274">School of Life Science and Agricultural Engineering, Nanyang Normal University, Nanyang 473061, China</mods:affiliation>
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<mods:namePart id="93EC26E65D7013B5FDC15A0C4229ECA9">Yan, Zhen-Li</mods:namePart>
<mods:affiliation id="5A9A5FB7F193F609587D9CEFB10BA6E6">State Key Laboratory of Motor Vehicle Biofuel Technology, Henan Tianguan Enterprise Group Co., Ltd., Nanyang 473000, China</mods:affiliation>
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<mods:namePart id="932F1F7051384F9850483C611949DA6E">Hui, Feng-Li</mods:namePart>
<mods:affiliation id="73DF91E5AF4A5330FADDEDE1CB438152">School of Life Science and Agricultural Engineering, Nanyang Normal University, Nanyang 473061, China &amp; Research Center of Henan Provincial Agricultural Biomass Resource Engineering and Technology, Nanyang 473061, China</mods:affiliation>
<mods:nameIdentifier id="5221BE5E987EB350ABC8DA1FB2B5E843" type="email">fenglihui@yeah.net</mods:nameIdentifier>
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<mods:date id="8D5939E55B604F6207F0D1F261D06BD6">2022</mods:date>
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<treatment id="57BF658EC9D558BC82D87077A00BF9EE" ID-GBIF-Taxon="195655705" LSID="urn:lsid:plazi:treatment:57BF658EC9D558BC82D87077A00BF9EE" httpUri="http://treatment.plazi.org/id/57BF658EC9D558BC82D87077A00BF9EE" lastPageNumber="1" pageId="0" pageNumber="1" scope_class="Saccharomycetes" scope_family="Trichomonascaceae" scope_order="Saccharomycetales">
<subSubSection id="3D38164E145CBDF0728EBF2D81AC2177" pageId="0" pageNumber="1" type="nomenclature">
<paragraph id="9E0EB544823CC78AB1CAEB91A0B5BC20" pageId="0" pageNumber="1">
<taxonomicName id="14145DC99C4433D77BA684565DBCAA77" ID-CoL="B2PMJ" LSID="57BF658E-C9D5-58BC-82D8-7077A00BF9EE" authority="C. Y. Chai &amp; F. L. Hui" authorityName="C. Y. Chai &amp; F. L. Hui" authorityYear="2022" class="Saccharomycetes" family="Trichomonascaceae" genus="Sugiyamaella" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Sugiyamaella cylindrica" order="Saccharomycetales" pageId="0" pageNumber="1" phylum="Ascomycota" rank="species" species="cylindrica" status="sp. nov.">Sugiyamaella cylindrica C.Y. Chai &amp; F.L. Hui</taxonomicName>
<taxonomicNameLabel id="7781FBFDDF86143479E0AAF4E5C03B77" pageId="0" pageNumber="1">sp. nov.</taxonomicNameLabel>
</paragraph>
</subSubSection>
<subSubSection id="BF9D48180DF2CD6500CB5F7BB7963BC3" pageId="0" pageNumber="1" type="description">
<paragraph id="750804AAB6EA2053490460816837E9C6" pageId="0" pageNumber="1">
<figureCitation id="02F04233FA84091282910BEFA52B1007" captionStart="Figure 3" captionStartId="F3" captionText="Figure 3. Morphology of Su. cylindrica (NYNU 201067, holotype) A budding cells were indicated by arrows in YM broth after 3 d B rudimentary pseudohyphae on CM agar after 14 d. Scale bars: 10 μm." figureDoi="10.3897/mycokeys.90.83829.figure3" httpUri="https://binary.pensoft.net/fig/688196" pageId="0" pageNumber="1">Fig. 3</figureCitation>
</paragraph>
</subSubSection>
<subSubSection id="D3A9FD78F4968F894F100BA3BCD20272" pageId="0" pageNumber="1" type="etymology">
<paragraph id="0CD65EEB096E014A4380FCC048736CD1" pageId="0" pageNumber="1">Etymology.</paragraph>
<paragraph id="AE876CA63E0860C6753BE0BEE6CA88E2" pageId="0" pageNumber="1">
The specific epithet
<emphasis id="5B1AFFBA224965394D4FAD94413A78B3" italics="true" pageId="0" pageNumber="1">cylindrica</emphasis>
refers to the cylindrical vegetative cells of the type strain.
</paragraph>
<caption id="4C610C6F5E1624E1FED59EE523276764" doi="10.3897/mycokeys.90.83829.figure3" httpUri="https://binary.pensoft.net/fig/688196" pageId="0" pageNumber="1" start="Figure 3" startId="F3">
<paragraph id="3B5524E01AD3CB183C4DECEA062E41B4" pageId="0" pageNumber="1">
<emphasis id="BFCA4650E85D854924CBF657B4E8CCAC" bold="true" pageId="0" pageNumber="1">Figure 3.</emphasis>
Morphology of
<taxonomicName id="7964BDB54930B4BF1AB19A39CA724B73" lsidName="Su. cylindrica" pageId="0" pageNumber="1" rank="species" species="cylindrica">
<emphasis id="230D2AD599E0BA052E4CAC71C7678561" italics="true" pageId="0" pageNumber="1">Su. cylindrica</emphasis>
</taxonomicName>
(NYNU 201067, holotype)
<emphasis id="C8DA0FCB76F353B5CAB6FA0785E93AF6" bold="true" pageId="0" pageNumber="1">A</emphasis>
budding cells were indicated by arrows in YM broth after 3 d
<emphasis id="C7571D0442945B00DC0FB76C190E32C8" bold="true" pageId="0" pageNumber="1">B</emphasis>
rudimentary pseudohyphae on CM agar after 14 d. Scale bars: 10
<normalizedToken id="FDA5E188AA07F90784BFC6D0892B6F72" originalValue="μm">μm</normalizedToken>
.
</paragraph>
</caption>
</subSubSection>
<subSubSection id="BA88FC5531C4A88E1D348A25F507834F" pageId="0" pageNumber="1" type="materials_examined">
<paragraph id="A71187B7793555F1791E21A4E67F2A5F" pageId="0" pageNumber="1">
<typeStatus id="26EC2DBCE3EF89CE8B4F5CC53CD24DE9">Type</typeStatus>
.
</paragraph>
<paragraph id="1EC0E53A20A38A3051DB596818DFB290" pageId="0" pageNumber="1">
<materialsCitation id="A22FEC2AEF19E71A45AA7C86780527EC" accessionNumber="CBS 16662" collectingDate="2020-10" collectorName="J. Z. Li, Z. T. Zhang" country="China" county="Song County" location="Tianchi Mountain National Forest Park" municipality="Luoyang City" specimenCount="1" stateProvince="Henan Province" typeStatus="holotype">
<collectingCountry id="AB0F074C8B2682E0BD45D8D73E7F6864" name="China">China</collectingCountry>
,
<collectingRegion id="5D23EAEE56E69129EAA89A1BB4C0FF31" country="China" name="Henan">Henan Province</collectingRegion>
,
<collectingMunicipality id="D920A62EE54F9B7ECBF4B7CC2291C462">Luoyang City</collectingMunicipality>
,
<collectingCounty id="EB3CBF1295B5762BF18D26B1FF8CCA85">Song County</collectingCounty>
, the
<location id="025E58BDCA5ADE6A612E03223D75AFCC" LSID="urn:lsid:plazi:treatment:57BF658EC9D558BC82D87077A00BF9EE:025E58BDCA5ADE6A612E03223D75AFCC" country="China" county="Song County" municipality="Luoyang City" name="Tianchi Mountain National Forest Park" stateProvince="Henan Province">
<location id="D15DAA209DD315BB4C59CB26F801D705" LSID="urn:lsid:plazi:treatment:57BF658EC9D558BC82D87077A00BF9EE:D15DAA209DD315BB4C59CB26F801D705" country="China" county="Song County" municipality="Luoyang City" name="Tianchi Mountain National Forest" stateProvince="Henan Province">
Tianchi
<location id="DD72EA92F69EB66610414D96FDE4AEE9" LSID="urn:lsid:plazi:treatment:57BF658EC9D558BC82D87077A00BF9EE:DD72EA92F69EB66610414D96FDE4AEE9" country="China" county="Song County" municipality="Luoyang City" name="Mountain National Forest Park" stateProvince="Henan Province">Mountain National Forest Park</location>
</location>
</location>
, in rotting wood,
<collectingDate id="CC56DD74D13265C55FA753407CFA9477" value="2020-10">October 2020</collectingDate>
,
<collectorName id="27E61FCB055549325749792AADB2EEEF">J.Z. Li</collectorName>
&amp;
<collectorName id="54130777C7BE193DB2D8929C436B0D52">Z.T. Zhang</collectorName>
(
<typeStatus id="E45E07048890510CE9E761B800F7CB6F">holotype</typeStatus>
NYNU 201067T, ex-type
<accessionNumber id="C64142A83393CB75C64F6734484418E8" httpUri="http://www.ncbi.nlm.nih.gov/protein/CBS16662">CBS 16662</accessionNumber>
= CICC 33514,
<typeStatus id="3054FD237B23B99739C19BA9F38AC0B4">holotype</typeStatus>
and ex-type are preserved in a metabolically inactive state)
</materialsCitation>
.
</paragraph>
</subSubSection>
<subSubSection id="F492A3E8A037B80F02F9F26A375F2717" pageId="0" pageNumber="1" type="description">
<paragraph id="07CE78C16D1CB5503D6ED676D12DF14C" pageId="0" pageNumber="1">Description.</paragraph>
<paragraph id="3D5C9B86400A7EED4DE7EE4B6E3A7209" pageId="0" pageNumber="1">
In YM broth after 3 days at 25 °C, cells are cylindrical (2-3
<normalizedToken id="34904863D386417D7E417DA6E365B6B1" originalValue="×">x</normalizedToken>
5-7
<normalizedToken id="8BF84D66DD45DDB5AAE92134E2CD747B" originalValue="μm">μm</normalizedToken>
) and occur singly or in pairs. Budding is multilateral. Sediment is formed after a month, but a pellicle is not observed. On YM agar after 3 days at 25 °C, colonies are white to cream-coloured, butyrous, convex and smooth with entire margins. In Dalmau plate culture on corn meal agar, rudimentary pseudohyphae are formed. Asci or signs of conjugation are not observed on sporulation media. Glucose and trehalose are weakly fermented, but, galactose, maltose sucrose, melibiose, lactose, cellobiose, melezitose, raffinose, inulin and xylose are not fermented. Glucose, galactose, l-sorbose, glucosamine, d-ribose, d-xylose, l-arabinose, d-arabinose, l-rhamnose, sucrose, maltose, trehalose, methyl
<normalizedToken id="07DAAAD82E2F5D5357B6B4248A56A669" originalValue="α-d-glucoside">α-d-glucoside</normalizedToken>
, cellobiose, salicin, melibiose, raffinose, melezitose, inulin, glycerol, erythritol, ribitol, d-glucitol, d-mannitol, galactitol,
<emphasis id="ABE1474CAFD03C87DF801B87453C8B2A" italics="true" pageId="0" pageNumber="1">myo</emphasis>
-inositol, d-glucono-1, 5-lactone, 2-keto-d-gluconate, 5-keto-d-gluconate, d-glucuronate, dl-lactate succinate, and ethanol are assimilated as sole carbon sources. Lactose, d-gluconate, citrate and methanol are not assimilated. Nitrate, nitrite, l-lysine, creatine, glucosamine, and d-tryptophan are assimilated as sole nitrogen sources. Ethylamine, cadaverine, creatinine, and imidazole are not assimilated. Minimum growth temperature is 15 °C, and maximum growth temperature is 35 °C. Growth in the presence of 0.1% cycloheximide is present, but growth in the presence of 1% acetic acid and 10% NaCl plus 5% glucose is absent. Starch-like compounds are not produced. Urease activity and diazonium blue B reactions are negative.
</paragraph>
</subSubSection>
<subSubSection id="5455AEFB312B5B90365337B92ED7B7F0" pageId="0" pageNumber="1" type="additional isolate examined">
<paragraph id="7E10CA3DAC7068F32E7012334519C512" pageId="0" pageNumber="1">Additional isolate examined.</paragraph>
<paragraph id="F27EBB79A0D5A63A8512465799BAB3A3" pageId="0" pageNumber="1">China, Henan Province, Luoyang City, Song County, the Tianchi Mountain National Forest Park, in rotting wood, October 2020, J.Z. Li &amp; Z.T. Zhang (NYNU 201034).</paragraph>
</subSubSection>
<subSubSection id="E20133E43C66357413386C8B90B0D43B" pageId="0" pageNumber="1" type="notes">
<paragraph id="30B826C6E6E81D5CA9F59812B828B1F3" pageId="0" pageNumber="1">Notes.</paragraph>
<paragraph id="4718516E7D3A558A320B4C6C69E4F722" pageId="0" pageNumber="1">
Two strains were collected from two different substrates, representing
<taxonomicName id="B83F07D697A4F8E05CC7B2211694F15B" lsidName="Su. cylindrica" pageId="0" pageNumber="1" rank="species" species="cylindrica">
<emphasis id="47A7998CE20380A589B814FD1D8F0FF7" italics="true" pageId="0" pageNumber="1">Su. cylindrica</emphasis>
</taxonomicName>
, clustered in the
<taxonomicName id="2ACEFD14F3687A647D737ACE19D1F6E2" class="Saccharomycetes" family="Trichomonascaceae" genus="Sugiyamaella" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Sugiyamaella" order="Saccharomycetales" pageId="0" pageNumber="1" phylum="Ascomycota" rank="genus">
<emphasis id="92B61237CF96802FD82785DF64ACC711" italics="true" pageId="0" pageNumber="1">Sugiyamaella</emphasis>
</taxonomicName>
clade and are closely related to
<taxonomicName id="2DB0FF5EF82DCD536B01D51C540B439F" lsidName="Su. marilandica" pageId="0" pageNumber="1" rank="species" species="marilandica">
<emphasis id="7DCECFB97BB0E9624F3FB3AE65C8B9E5" italics="true" pageId="0" pageNumber="1">Su. marilandica</emphasis>
</taxonomicName>
and
<taxonomicName id="EB3D6C4437110948BD5090622B5A7F56" lsidName="Su. qingdaonensis" pageId="0" pageNumber="1" rank="species" species="qingdaonensis">
<emphasis id="8FEE61660D3B36575BFC426AC8A831BA" italics="true" pageId="0" pageNumber="1">Su. qingdaonensis</emphasis>
</taxonomicName>
. The nucleotide differences between the new species and the close relatives
<taxonomicName id="5021AA800B3A5940E1A4C90202A5404F" lsidName="Su. marilandica" pageId="0" pageNumber="1" rank="species" species="marilandica">
<emphasis id="71277E559A9138AAD84DD5ACABC59362" italics="true" pageId="0" pageNumber="1">Su. marilandica</emphasis>
</taxonomicName>
and
<taxonomicName id="11B5C7EA3B5F3AAC2E2EB70064FEAF6F" lsidName="Su. qingdaonensis" pageId="0" pageNumber="1" rank="species" species="qingdaonensis">
<emphasis id="367EC1A0E1D1970AD9A08804A94B0546" italics="true" pageId="0" pageNumber="1">Su. qingdaonensis</emphasis>
</taxonomicName>
are 1.1-1.4% substitutions in the D1/D2 domain and 5.0-5.9% substitutions in the ITS region, respectively. Physiologically,
<taxonomicName id="5963E58F46FAA081FDD54D248828C7C5" lsidName="Su. cylindrica" pageId="0" pageNumber="1" rank="species" species="cylindrica">
<emphasis id="72AD3FC4EF67087D04E11C287E72C0CD" italics="true" pageId="0" pageNumber="1">Su. cylindrica</emphasis>
</taxonomicName>
differs from the closely related species
<taxonomicName id="4D1DEFA52458D97206563E0FA1431EED" lsidName="Su. marilandica" pageId="0" pageNumber="1" rank="species" species="marilandica">
<emphasis id="51653A4E8CD3FC63E34CF9CD5CEDF878" italics="true" pageId="0" pageNumber="1">Su. marilandica</emphasis>
</taxonomicName>
and
<taxonomicName id="5CDA3679CB9CBF3BC359F24CD6728673" lsidName="Su. qingdaonensis" pageId="0" pageNumber="1" rank="species" species="qingdaonensis">
<emphasis id="D7819492DC746B79AC4FFB7239517707" italics="true" pageId="0" pageNumber="1">Su. qingdaonensis</emphasis>
</taxonomicName>
(
<bibRefCitation id="C845895AA25551DA04608D36967F779C" DOI="https://doi.org/10.1099/ijs.0.015230-0" author="Wang, SA" journalOrPublisher="International Journal of Systematic and Evolutionary Microbiology" pageId="0" pageNumber="1" pagination="1697 - 1701" refId="B35" refString="Wang, SA, Li, FL, Bai, FY, 2010. Candida laoshanensis sp. nov. and Candida qingdaonensis sp. nov., anamorphic, ascomycetous yeast species isolated from decayed wood. International Journal of Systematic and Evolutionary Microbiology 60 (7): 1697 - 1701, DOI: https://doi.org/10.1099/ijs.0.015230-0" title="Candida laoshanensis sp. nov. and Candida qingdaonensis sp. nov., anamorphic, ascomycetous yeast species isolated from decayed wood." url="https://doi.org/10.1099/ijs.0.015230-0" volume="60" year="2010">Wang et al. 2010</bibRefCitation>
;
<bibRefCitation id="D51068650ED1AEDD768DEF604D890633" DOI="https://doi.org/10.1016/B978-0-444-52149-1.00072-0" author="Kurtzman, CP" editor="Kurtzman, CP" journalOrPublisher=", Vol. 2. Elsevier, Amsterdam" pageId="0" pageNumber="1" pagination="817 - 822" refId="B13" refString="Kurtzman, CP, 2011b. Sugiyamaella Kurtzman &amp; Robnett (2007). In: Kurtzman, CP, Fell, JW, Boekhout, T, Eds., The Yeasts - a Taxonomic Study, 5th edn., Vol. 2. Elsevier, Amsterdam: 817 - 822, DOI: https://doi.org/10.1016/B978-0-444-52149-1.00072-0" title="Sugiyamaella Kurtzman &amp; Robnett (2007)." url="https://doi.org/10.1016/B978-0-444-52149-1.00072-0" volumeTitle="The Yeasts - a Taxonomic Study, 5 th edn." year="2011 b">Kurtzman 2011b</bibRefCitation>
) in its ability to assimilate glycerol and dl-lactate and to grow at 35 °C. Additionally, the new species ferments trehalose, while
<emphasis id="A9EEA8A901D5DF72E4CFFE2B83C3DE39" italics="true" pageId="0" pageNumber="1">
<taxonomicName id="10B326ACF2373E95819A680CE43E10FA" lsidName="Su. marilandica" pageId="0" pageNumber="1" rank="species" species="marilandica">Su. marilandica</taxonomicName>
and
<taxonomicName id="F1F5883FDF9CA1F42A19F9E201A67C18" lsidName="Su. qingdaonensis" pageId="0" pageNumber="1" rank="species" species="qingdaonensis">Su. qingdaonensis</taxonomicName>
</emphasis>
do not.
</paragraph>
</subSubSection>
</treatment>
</document>