treatments-xml/data/03/FB/F1/03FBF116846AFFEDFD89FDF16946FBC5.xml

<document id="AE941A207D7E58DBA4F086E98F981111" ID-CLB-Dataset="58894" ID-DOI="10.5852/ejt.2023.861.2063" ID-GBIF-Dataset="98f15b31-8fcd-4ea4-9621-6bff596e64fe" ID-ISSN="2118-9773" ID-Zenodo-Dep="7710258" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.taxonomicNames_approvedBy="felipe" checkinTime="1678329856084" checkinUser="felipe" docAuthor="Véron, Benoît, Rougier, Etienne, Taylor, Anthony &amp; Goux, Didier" docDate="2023" docId="03FBF116846AFFEDFD89FDF16946FBC5" docLanguage="en" docName="ejt-861_21-47.pdf" docOrigin="European Journal of Taxonomy 861" docStyle="DocumentStyle:EF2B578F1D15862ADE45B0C07C620911.14:EJT.2018-.journal_article.type1" docStyleId="EF2B578F1D15862ADE45B0C07C620911" docStyleName="EJT.2018-.journal_article.type1" docStyleVersion="14" docTitle="Exanthemachrysis fresneliae Veron 2023, sp. nov." docType="treatment" docVersion="4" lastPageNumber="29" masterDocId="FFC2896E846FFFE5FF98FFD16A26FF91" masterDocTitle="New species of Pavlovophyceae (Haptophyta) and revision of the genera Exanthemachrysis, Rebecca and Pavlova" masterLastPageNumber="47" masterPageNumber="21" pageNumber="26" updateTime="1699476326315" updateUser="ExternalLinkService" zenodo-license-document="CC-BY-4.0" zenodo-license-figures="CC-BY-4.0" zenodo-license-treatments="UNSPECIFIED">
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<mods:title id="AE8E61603C58FFE1DC29DE8F02AF33CE">New species of Pavlovophyceae (Haptophyta) and revision of the genera Exanthemachrysis, Rebecca and Pavlova</mods:title>
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<mods:namePart id="C6D499A1938DC6C303195923478300EF">Véron, Benoît</mods:namePart>
<mods:affiliation id="5BEE056AC225156083B9B00402014B45">Laboratoire de Biologie des Organismes et Écosystèmes Aquatiques (BOREA) Université de Caen-Normandie, MNHN, SU, UA, CNRS, IRD, 14000 Caen, France. &amp; Normandie Université, UNICAEN, UMR 8067 BOREA, MNHN, SU, CNRS, IRD- 207, UA, 14000 Caen, France.</mods:affiliation>
<mods:nameIdentifier id="4F0B3C10B806BDE3ACDB2D64A38805E3" type="email">benoit.veron@unicaen.fr</mods:nameIdentifier>
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<mods:namePart id="FB08BFA489751F9DB3175968D875A57B">Rougier, Etienne</mods:namePart>
<mods:affiliation id="E91F87DDDA9C35144B8BBF3ACB34DB6A">Laboratoire de Biologie des Organismes et Écosystèmes Aquatiques (BOREA) Université de Caen-Normandie, MNHN, SU, UA, CNRS, IRD, 14000 Caen, France. &amp; Normandie Université, UNICAEN, UMR 8067 BOREA, MNHN, SU, CNRS, IRD- 207, UA, 14000 Caen, France.</mods:affiliation>
<mods:nameIdentifier id="BD6E0AD94F9EBB7821F6CCF593543BC6" type="email">etienne.rougier@gmail.com</mods:nameIdentifier>
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<mods:namePart id="0DF2E1F611D5EF670D11CB0A3B848D04">Taylor, Anthony</mods:namePart>
<mods:affiliation id="0A4BCD04B66EA9EC3E747127A9D64D01">Laboratoire de Biologie des Organismes et Écosystèmes Aquatiques (BOREA) Université de Caen-Normandie, MNHN, SU, UA, CNRS, IRD, 14000 Caen, France. &amp; Normandie Université, UNICAEN, UMR 8067 BOREA, MNHN, SU, CNRS, IRD- 207, UA, 14000 Caen, France. &amp; Institute of Marine Sciences, University of Portsmouth, Portsmouth, Hampshire, UK.</mods:affiliation>
<mods:nameIdentifier id="CA64BCF23A8DA2EEC5C3CD1DF9F44D6A" type="email">anthony.taylor2590@gmail.com</mods:nameIdentifier>
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<mods:namePart id="00AB4AC414D3268A59F352878B8EE705">Goux, Didier</mods:namePart>
<mods:affiliation id="7E9EADCC61061477E2FCF03BDCE70AC2">Normandie Université, UNICAEN, CMAbio 3 (Centre de Microscopie Appliquée à la Biologie), SF EMerode, 14000 Caen, France. &amp; Normandie Université, ENSICAEN, UNICAEN, CNRS, UMR 6508 CRISMAT, 14000 Caen, France.</mods:affiliation>
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<mods:title id="9080BE8066EA91A8BF43E1DADD5AF2C3">European Journal of Taxonomy</mods:title>
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<mods:date id="5E5C7A6ABE94514BF85ACC2E948C951F">2023</mods:date>
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<treatment id="03FBF116846AFFEDFD89FDF16946FBC5" ID-DOI="http://doi.org/10.5281/zenodo.7713649" ID-GBIF-Taxon="209434623" ID-Zenodo-Dep="7713649" LSID="urn:lsid:plazi:treatment:03FBF116846AFFEDFD89FDF16946FBC5" httpUri="http://treatment.plazi.org/id/03FBF116846AFFEDFD89FDF16946FBC5" lastPageId="8" lastPageNumber="29" pageId="5" pageNumber="26">
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<paragraph id="8BED4000846AFFE0FD89FDF16E05FDAD" blockId="5.[529,1059,544,639]" box="[529,1059,544,572]" pageId="5" pageNumber="26">
<taxonomicName id="4C523B83846AFFE0FD89FDF169E7FDAA" authority="Veron" authorityName="Veron" authorityYear="2023" box="[529,961,544,571]" class="Pavlovophyceae" family="Pavlovaceae" genus="Exanthemachrysis" kingdom="Chromista" order="Pavlovales" pageId="5" pageNumber="26" phylum="Haptophyta" rank="species" species="fresneliae" status="sp. nov.">
<emphasis id="B9269C12846AFFE0FD89FDF16956FDAA" bold="true" box="[529,880,544,571]" italics="true" pageId="5" pageNumber="26">Exanthemachrysis fresneliae</emphasis>
Véron
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<taxonomicNameLabel id="A2152169846AFFE0FC50FDF36E05FDAD" box="[968,1059,546,572]" pageId="5" pageNumber="26" rank="species">sp. nov.</taxonomicNameLabel>
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<subSubSection id="C348138B846AFFE0FD3AFD93696BFDEE" pageId="5" pageNumber="26" type="description">
<paragraph id="8BED4000846AFFE0FD3AFD9369B7FDCD" blockId="5.[529,1059,544,639]" box="[674,913,578,604]" pageId="5" pageNumber="26">PhycoBank: 103626</paragraph>
<paragraph id="8BED4000846AFFE0FD7FFDB4696BFDEE" blockId="5.[529,1059,544,639]" box="[743,845,613,639]" pageId="5" pageNumber="26">
<figureCitation id="13695C85846AFFE0FD7FFDB4696BFDEE" box="[743,845,613,639]" captionStart-0="Fig" captionStart-1="Fig" captionStart-2="Fig" captionStart-3="Fig" captionStart-4="Fig" captionStart-5="Fig" captionStartId-0="5.[189,232,1787,1813]" captionStartId-1="6.[189,232,1822,1848]" captionStartId-2="7.[189,232,1857,1883]" captionStartId-3="8.[189,232,1823,1849]" captionStartId-4="9.[189,232,1693,1719]" captionStartId-5="10.[189,232,843,869]" captionTargetBox-0="[290,1298,831,1748]" captionTargetBox-1="[189,1398,568,1783]" captionTargetBox-2="[188,1397,738,1819]" captionTargetBox-3="[189,1398,1247,1786]" captionTargetBox-4="[189,1398,265,1656]" captionTargetBox-5="[189,1398,265,802]" captionTargetId-0="figure-82@5.[290,1298,831,1748]" captionTargetId-1="figure-82@6.[189,1398,568,1783]" captionTargetId-2="figure-97@7.[188,1397,738,1819]" captionTargetId-3="figure-343@8.[189,1398,1247,1786]" captionTargetId-4="figure-11@9.[189,1398,265,1656]" captionTargetId-5="figure-179@10.[189,1398,265,802]" captionTargetPageId-0="5" captionTargetPageId-1="6" captionTargetPageId-2="7" captionTargetPageId-3="8" captionTargetPageId-4="9" captionTargetPageId-5="10" captionText-0="Fig. 1. Exanthemachrysis fresneliae Véron sp. nov. (AC35), palmelloid and swimming cells, LM images. A. Benthic colonies of non-motile sister cells embedded in non-stratified M (black arrows) with storage granules of P (white arrows). B. Benthic non-motile cell embedded in non-stratified M (black arrow) with storage granule of P (white arrow), bilobed C and anterior bulging PY. C. Apical view (bottom right) and side view (up left) of 2 swimming cells with conspicuous S-shaped AF (black arrows) emerging almost mid-ventrally from a pit (white arrows). D. Side view of motile cells with bulging PY (black arrows). Scale bars: A = 5 µm; B = 1 µm; C–D = 2 µm." captionText-1="Fig. 2. Exanthemachrysis fresneliae Véron sp. nov. (AC35), palmelloid sister cells, TEM image (Fix 1). Benthic colony of non-motile sister cells embedded in a common non-stratified M envelope (white stars); parietal C with helicoidal arrangement of the TH lamellae and a bulging PY near the F bases perpendicular to each other (white arrows), with the basal bodies connecting to the microtubular root (black arrows) running to the central area of the cell. Secretory vesicles from G (black star) ready to discharge content near the pit where the F emerge. Scale bar: 0.5 µm." captionText-2="Fig. 3. Exanthemachrysis fresneliae Véron sp. nov. (AC35), palmelloid cell, TEM image (Fix 1). Nonmotile benthic cell embedded in non-stratified M showing single parietal C with a bulging PY at its end. Row of few osmiophilic vesicles forming an E in C stroma located at the junction of TH and PY. Both F bases (white arrows) positioned in continuity with a notch in the nucleus facing the sub-ventral pit (black arrow). Scale bar:1 µm." captionText-3="Fig. 4. Exanthemachrysis fresneliae Véron sp. nov. (AC35), flagellar apparatus of motile cells. A. SEM image of side view of swimming cell with a 3–4 µm long conspicuous sinusoidal S-shaped and slightly tapering AF emerging almost mid-ventrally and thickly covered by KS (white arrows). B–C. Negative staining image. B. Detail of the 3 appendices, the AF, the H and the PF composed of 2 parts of sub-equal lengths with a distal attenuation. C. Detail of the AF of a cell bearing ovoid KS (black arrows) down to the base, in regular helicoidal rows. Scale bars: A–B = 1 µm; C = 0.1 µm." captionText-4="Fig. 5. Exanthemachrysis fresneliae Véron sp. nov. (AC35), flagella, plasma membrane and knob-scales, TEM images (Fix 1). A. Longitudinal section of the pit area with 2 emerging F (white arrows = central microtubules) surrounded by a sheath of reticulum enclosing their bases. Plasma membrane (black arrow), underlined with endoplasmic reticulum (white stars), covered with KS. B. Details of the cell surface in the region of F emergence. Oblique sections of F bases with central microtubules (white arrow). Stalked ovoid KS partially covering the F bases and the plasma membrane. C. G with cisternae and secretory vesicles containing KS (white arrows). Scale bars: A–B = 50 nm; C = 0.5 µm." captionText-5="Fig. 6. Exanthemachrysis fresneliae Véron sp. nov. (AC35), viroplasm, TEM images (Fix 1). A. Benthic non-motile cell embedded in M; parietal C lobes with helicoidal arrangement of the TH lamellae. Cytoplasmic assembly of pentahedral virus (V). B. Viral particles scattered in the cytoplasm. Scale bars: A = 0.2 µm; B = 50 nm." figureDoi-0="http://doi.org/10.5281/zenodo.7710260" figureDoi-1="http://doi.org/10.5281/zenodo.7710262" figureDoi-2="http://doi.org/10.5281/zenodo.7710264" figureDoi-3="http://doi.org/10.5281/zenodo.7710266" figureDoi-4="http://doi.org/10.5281/zenodo.7710268" figureDoi-5="http://doi.org/10.5281/zenodo.7710270" httpUri-0="https://zenodo.org/record/7710260/files/figure.png" httpUri-1="https://zenodo.org/record/7710262/files/figure.png" httpUri-2="https://zenodo.org/record/7710264/files/figure.png" httpUri-3="https://zenodo.org/record/7710266/files/figure.png" httpUri-4="https://zenodo.org/record/7710268/files/figure.png" httpUri-5="https://zenodo.org/record/7710270/files/figure.png" pageId="5" pageNumber="26">Figs 1–6</figureCitation>
</paragraph>
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<subSubSection id="C348138B846AFFE3FF25FD74692DFED6" lastPageId="6" lastPageNumber="27" pageId="5" pageNumber="26" type="diagnosis">
<paragraph id="8BED4000846AFFE0FF25FD746B10FD2E" blockId="5.[189,310,677,703]" box="[189,310,677,703]" pageId="5" pageNumber="26">
<heading id="D0A5F76C846AFFE0FF25FD746B10FD2E" bold="true" box="[189,310,677,703]" fontSize="11" level="3" pageId="5" pageNumber="26" reason="3">
<emphasis id="B9269C12846AFFE0FF25FD746B10FD2E" bold="true" box="[189,310,677,703]" pageId="5" pageNumber="26">Diagnosis</emphasis>
</heading>
</paragraph>
<paragraph id="8BED4000846AFFE3FF25FD03692DFED6" blockId="5.[189,1399,722,782]" lastBlockId="6.[189,1399,265,327]" lastPageId="6" lastPageNumber="27" pageId="5" pageNumber="26">
The new species differs from the only species of the genus, i.e., 
<taxonomicName id="4C523B83846AFFE0FC5AFD036F32FD7D" box="[962,1300,722,748]" class="Pavlovophyceae" family="Pavlovaceae" genus="Exanthemachrysis" kingdom="Chromista" order="Pavlovales" pageId="5" pageNumber="26" phylum="Haptophyta" rank="species" species="gayraliae">
<emphasis id="B9269C12846AFFE0FC5AFD036F32FD7D" box="[962,1300,722,748]" italics="true" pageId="5" pageNumber="26">Exanthemachrysis gayraliae</emphasis>
</taxonomicName>
, mainly by the presence of ovoid pedunculated knob-scales arranged helically on the AF but also by a slightly shorter AF. The benthic stage is pseudo-palmeloid. The pyrenoid is in 
<taxonomicName id="4C523B838469FFE3FC66FEDB6EB4FEB2" authorityName="Veron" authorityYear="2023" box="[1022,1170,265,291]" class="Pavlovophyceae" family="Pavlovaceae" genus="Exanthemachrysis" kingdom="Chromista" order="Pavlovales" pageId="6" pageNumber="27" phylum="Haptophyta" rank="species" species="fresneliae">
<emphasis id="B9269C128469FFE3FC66FEDB6EB4FEB2" box="[1022,1170,265,291]" italics="true" pageId="6" pageNumber="27">E. fresneliae</emphasis>
</taxonomicName>
at the end of the C whereas it is in the centre of the C in 
<taxonomicName id="4C523B838469FFE3FDEBFEFC6922FED7" box="[627,772,300,326]" class="Pavlovophyceae" family="Pavlovaceae" genus="Exanthemachrysis" kingdom="Chromista" order="Pavlovales" pageId="6" pageNumber="27" phylum="Haptophyta" rank="species" species="gayraliae">
<emphasis id="B9269C128469FFE3FDEBFEFC6922FED7" box="[627,772,300,326]" italics="true" pageId="6" pageNumber="27">E. gayraliae</emphasis>
</taxonomicName>
.
</paragraph>
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<paragraph id="8BED4000846AFFE0FF25F92A68F8F878" blockId="5.[188,1399,1787,2025]" pageId="5" pageNumber="26">
<emphasis id="B9269C12846AFFE0FF25F92A6B2FF884" bold="true" box="[189,265,1787,1813]" pageId="5" pageNumber="26">Fig. 1.</emphasis>
<taxonomicName id="4C523B83846AFFE0FE95F92A688CF884" authority="Veron" authorityName="Veron" authorityYear="2023" box="[269,682,1787,1813]" class="Pavlovophyceae" family="Pavlovaceae" genus="Exanthemachrysis" kingdom="Chromista" order="Pavlovales" pageId="5" pageNumber="26" phylum="Haptophyta" rank="species" species="fresneliae" status="sp. nov.">
<emphasis id="B9269C12846AFFE0FE95F92A687DF884" box="[269,603,1787,1813]" italics="true" pageId="5" pageNumber="26">Exanthemachrysis fresneliae</emphasis>
Véron
</taxonomicName>
<taxonomicNameLabel id="A2152169846AFFE0FD28F92D692FF887" box="[688,777,1788,1814]" pageId="5" pageNumber="26" rank="species">sp. nov.</taxonomicNameLabel>
(AC35), palmelloid and swimming cells, LM images. 
<emphasis id="B9269C12846AFFE0FF25F8CF6AF4F8A9" bold="true" box="[189,210,1822,1848]" pageId="5" pageNumber="26">A</emphasis>
. Benthic colonies of non-motile sister cells embedded in non-stratified M (black arrows) with storage granules of P (white arrows). 
<emphasis id="B9269C12846AFFE0FD97F8936804F8CD" bold="true" box="[527,546,1858,1884]" pageId="5" pageNumber="26">B</emphasis>
. Benthic non-motile cell embedded in non-stratified M (black arrow) with storage granule of P (white arrow), bilobed C and anterior bulging PY. 
<emphasis id="B9269C12846AFFE0FC76F8B56E25F8EF" bold="true" box="[1006,1027,1892,1918]" pageId="5" pageNumber="26">C</emphasis>
. Apical view (bottom right) and side view (up left) of 2 swimming cells with conspicuous S-shaped AF (black arrows) emerging almost mid-ventrally from a pit (white arrows). 
<emphasis id="B9269C12846AFFE0FD3AF87A6890F854" bold="true" box="[674,694,1963,1989]" pageId="5" pageNumber="26">D</emphasis>
. Side view of motile cells with bulging PY (black arrows). Scale bars: A = 5 µm; B = 1 µm; C–D = 2 µm.
</paragraph>
</caption>
<subSubSection id="C348138B8469FFE3FF25FEBF6B75FE6D" pageId="6" pageNumber="27" type="etymology">
<paragraph id="8BED40008469FFE3FF25FEBF6B62FE19" blockId="6.[189,324,366,392]" box="[189,324,366,392]" pageId="6" pageNumber="27">
<heading id="D0A5F76C8469FFE3FF25FEBF6B62FE19" bold="true" box="[189,324,366,392]" fontSize="11" level="3" pageId="6" pageNumber="27" reason="3">
<emphasis id="B9269C128469FFE3FF25FEBF6B62FE19" bold="true" box="[189,324,366,392]" pageId="6" pageNumber="27">Etymology</emphasis>
</heading>
</paragraph>
<paragraph id="8BED40008469FFE3FF25FE4D6B75FE6D" blockId="6.[189,1399,412,508]" pageId="6" pageNumber="27">
The epithet given to the species is dedicated in honour of Dr Jacqueline Fresnel who worked at the Université de Caen 
<collectingRegion id="49968EE28469FFE3FE3AFE6E680FFE48" box="[418,553,447,473]" country="France" name="Haute-Normandie" pageId="6" pageNumber="27">Normandie</collectingRegion>
, reinvestigated in details 
<taxonomicName id="4C523B838469FFE3FCD6FE1169F8FE48" box="[846,990,447,473]" class="Pavlovophyceae" family="Pavlovaceae" genus="Exanthemachrysis" kingdom="Chromista" order="Pavlovales" pageId="6" pageNumber="27" phylum="Haptophyta" rank="species" species="gayraliae">
<emphasis id="B9269C128469FFE3FCD6FE1169F8FE48" box="[846,990,447,473]" italics="true" pageId="6" pageNumber="27">E. gayraliae</emphasis>
</taxonomicName>
and who described 22 taxa, mostly haptophytes.
</paragraph>
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<paragraph id="8BED40008469FFE3FF25F8CF6933F878" blockId="6.[189,1399,1822,2025]" pageId="6" pageNumber="27">
<emphasis id="B9269C128469FFE3FF25F8CF6B2CF8A9" bold="true" box="[189,266,1822,1848]" pageId="6" pageNumber="27">Fig. 2.</emphasis>
<taxonomicName id="4C523B838469FFE3FE88F8CF6896F8A9" authority="Veron" authorityName="Veron" authorityYear="2023" box="[272,688,1822,1848]" class="Pavlovophyceae" family="Pavlovaceae" genus="Exanthemachrysis" kingdom="Chromista" order="Pavlovales" pageId="6" pageNumber="27" phylum="Haptophyta" rank="species" species="fresneliae" status="sp. nov.">
<emphasis id="B9269C128469FFE3FE88F8CF6847F8A9" box="[272,609,1822,1848]" italics="true" pageId="6" pageNumber="27">Exanthemachrysis fresneliae</emphasis>
Véron
</taxonomicName>
<taxonomicNameLabel id="A21521698469FFE3FD2FF8CE6937F8A8" box="[695,785,1823,1849]" pageId="6" pageNumber="27" rank="species">sp. nov.</taxonomicNameLabel>
(AC35), palmelloid sister cells, TEM image (Fix 1). Benthic colony of non-motile sister cells embedded in a common non-stratified M envelope (white stars); parietal C with helicoidal arrangement of the TH lamellae and a bulging PY near the F bases perpendicular to each other (white arrows), with the basal bodies connecting to the microtubular root (black arrows) running to the central area of the cell. Secretory vesicles from G (black star) ready to discharge content near the pit where the F emerge. Scale bar: 0.5 µm.
</paragraph>
</caption>
<subSubSection id="C348138B8468FFE2FF24FED86F72FE09" pageId="7" pageNumber="28" type="materials_examined">
<paragraph id="8BED40008468FFE2FF24FED86B4CFEB3" blockId="7.[188,362,264,291]" box="[188,362,264,291]" pageId="7" pageNumber="28">
<heading id="D0A5F76C8468FFE2FF24FED86B4CFEB3" bold="true" box="[188,362,264,291]" fontSize="11" level="3" pageId="7" pageNumber="28" reason="3">
<emphasis id="B9269C128468FFE2FF24FED86B4CFEB3" bold="true" box="[188,362,264,291]" pageId="7" pageNumber="28">
<typeStatus id="54E9FEA28468FFE2FF24FED86ADFFEB2" box="[188,249,265,291]" pageId="7" pageNumber="28">Type</typeStatus>
material
</emphasis>
</heading>
</paragraph>
<paragraph id="8BED40008468FFE2FF71FEE66F72FE09" blockId="7.[188,1405,311,408]" pageId="7" pageNumber="28">
<materialsCitation id="3B3A4A5D8468FFE2FF71FEE66F72FE09" ID-GBIF-Occurrence="4052628301" collectorName="Jacqueline Fresnel" country="France" latitude="48.866665" location="Chausey Islands" longLatPrecision="1107" longitude="-1.8166666" municipality="Chausey Islands" pageId="7" pageNumber="28" specimenCount="1" stateProvince="Normandy" typeStatus="holotype">
<emphasis id="B9269C128468FFE2FF71FEE66B7DFEC0" bold="true" box="[233,347,311,337]" pageId="7" pageNumber="28">
<typeStatus id="54E9FEA28468FFE2FF71FEE66B7DFEC0" box="[233,347,311,337]" pageId="7" pageNumber="28" type="holotype">Holotype</typeStatus>
</emphasis>
<collectingCountry id="F34500908468FFE2FF24FE8B6B16FEE5" box="[188,304,346,372]" name="France" pageId="7" pageNumber="28">FRANCE</collectingCountry>
• 
<collectingRegion id="49968EE28468FFE2FEDEFE8B6BE3FEE5" box="[326,453,346,372]" country="France" name="Haute-Normandie" pageId="7" pageNumber="28">Normandy</collectingRegion>
, 
<collectingMunicipality id="6B89DA7A8468FFE2FE57FE8B68B6FEE5" box="[463,656,346,372]" pageId="7" pageNumber="28">Chausey Islands</collectingMunicipality>
, Epi-silty; 
<geoCoordinate id="EE6626C78468FFE2FC96FE88695FFEE4" box="[782,889,345,373]" degrees="48" direction="north" minutes="52" orientation="latitude" pageId="7" pageNumber="28" precision="925" value="48.866665">48°52′ N</geoCoordinate>
, 
<geoCoordinate id="EE6626C78468FFE2FC1BFE8869C3FEE4" box="[899,997,345,373]" degrees="1" direction="west" minutes="49" orientation="longitude" pageId="7" pageNumber="28" precision="925" value="-1.8166666">1°49′ W</geoCoordinate>
; alt. 0 m; 1974; 
<collectorName id="26A725D68468FFE2FB05FE8B6F5BFEE5" box="[1181,1405,346,372]" pageId="7" pageNumber="28">Jacqueline Fresnel</collectorName>
leg.; Jacqueline Fresnel isol.; AC35; GenBank nos: 
<accessionNumber id="9401DDE38468FFE2FC83FEAF69B7FE09" box="[795,913,382,408]" pageId="7" pageNumber="28">JF714227</accessionNumber>
, 
<accessionNumber id="9401DDE38468FFE2FC05FEAC6E33FE06" box="[925,1045,381,407]" pageId="7" pageNumber="28">JF718767</accessionNumber>
; 
<accessionNumber id="9401DDE38468FFE2FBB8FEAC6E88FE09" box="[1056,1198,381,408]" pageId="7" pageNumber="28">CN[35NM1</accessionNumber>
– 
<accessionNumber id="9401DDE38468FFE2FB52FEAC6F69FE09" box="[1226,1359,381,408]" pageId="7" pageNumber="28">AC35-1M]</accessionNumber>
.
</materialsCitation>
</paragraph>
</subSubSection>
<subSubSection id="C348138B8468FFEDFF24FE6E6946FBC5" lastPageId="8" lastPageNumber="29" pageId="7" pageNumber="28" type="description">
<paragraph id="8BED40008468FFE2FF24FE6E6B6BFE48" blockId="7.[188,333,447,473]" box="[188,333,447,473]" pageId="7" pageNumber="28">
<heading id="D0A5F76C8468FFE2FF24FE6E6B6BFE48" bold="true" box="[188,333,447,473]" fontSize="11" level="3" pageId="7" pageNumber="28" reason="3">
<emphasis id="B9269C128468FFE2FF24FE6E6B6BFE48" bold="true" box="[188,333,447,473]" pageId="7" pageNumber="28">Description</emphasis>
</heading>
</paragraph>
<paragraph id="8BED40008468FFE2FF24FE3C6B2FFD05" blockId="7.[188,1398,493,660]" pageId="7" pageNumber="28">Metabolic non-phototactic slightly bean-shaped motile cells. Single green-brownish chloroplast with a bulging PY, helicoidal and parallel thylakoids with an eyespot located at the thylakoids-pyrenoid interface. Sinusoidal anterior flagellum bears stalked knob-scales, rigid posterior flagellum with a long distal attenuation and a short haptonema. Cell membrane strewn with same shape and same size knobscales.</paragraph>
<caption id="DF2D10888468FFE2FF25F8906802F879" ID-DOI="http://doi.org/10.5281/zenodo.7710264" ID-Zenodo-Dep="7710264" httpUri="https://zenodo.org/record/7710264/files/figure.png" pageId="7" pageNumber="28" startId="7.[189,232,1857,1883]" targetBox="[188,1397,738,1819]" targetPageId="7">
<paragraph id="8BED40008468FFE2FF25F8906802F879" blockId="7.[189,1399,1857,2024]" pageId="7" pageNumber="28">
<emphasis id="B9269C128468FFE2FF25F8906B2DF8CA" bold="true" box="[189,267,1857,1883]" pageId="7" pageNumber="28">Fig. 3.</emphasis>
<taxonomicName id="4C523B838468FFE2FE8BF8906892F8CA" authority="Veron" authorityName="Veron" authorityYear="2023" box="[275,692,1857,1883]" class="Pavlovophyceae" family="Pavlovaceae" genus="Exanthemachrysis" kingdom="Chromista" order="Pavlovales" pageId="7" pageNumber="28" phylum="Haptophyta" rank="species" species="fresneliae" status="sp. nov.">
<emphasis id="B9269C128468FFE2FE8BF8906843F8CA" box="[275,613,1857,1883]" italics="true" pageId="7" pageNumber="28">Exanthemachrysis fresneliae</emphasis>
Véron
</taxonomicName>
<taxonomicNameLabel id="A21521698468FFE2FD24F8906931F8CA" box="[700,791,1857,1883]" pageId="7" pageNumber="28" rank="species">sp. nov.</taxonomicNameLabel>
(AC35), palmelloid cell, TEM image (Fix 1). Nonmotile benthic cell embedded in non-stratified M showing single parietal C with a bulging PY at its end. Row of few osmiophilic vesicles forming an E in C stroma located at the junction of TH and PY. Both F bases (white arrows) positioned in continuity with a notch in the nucleus facing the sub-ventral pit (black arrow). Scale bar:1 µm.
</paragraph>
</caption>
<paragraph id="8BED40008467FFEDFF25FED86E8BFEB2" blockId="8.[189,1197,265,291]" box="[189,1197,265,291]" pageId="8" pageNumber="29">The non-motile palmelloid cells form colonies surrounded by non-stratified mucilage.</paragraph>
<paragraph id="8BED40008467FFEDFF25FE9B6869FEF5" blockId="8.[189,591,330,357]" box="[189,591,330,357]" pageId="8" pageNumber="29">
<heading id="D0A5F76C8467FFEDFF25FE9B6869FEF5" bold="true" box="[189,591,330,357]" fontSize="11" level="3" pageId="8" pageNumber="29" reason="3">
<emphasis id="B9269C128467FFEDFF25FE9B6869FEF5" bold="true" box="[189,591,330,357]" pageId="8" pageNumber="29">Microscopy and related analysis</emphasis>
</heading>
</paragraph>
<paragraph id="8BED40008467FFEDFF25FEA869C1FE48" blockId="8.[189,1398,377,474]" pageId="8" pageNumber="29">
The dominant stage is palmelloid with slightly ovoid cells (4.80 µm ± 0.57 × 4.21 µm ± 0.56, n = 51) forming colonies of sister cells remaining coated with a common non-stratified mucilage (
<figureCitation id="13695C858467FFEDFB74FE4D6F57FE27" box="[1260,1393,412,438]" captionStart="Fig" captionStartId="5.[189,232,1787,1813]" captionTargetBox="[290,1298,831,1748]" captionTargetId="figure-82@5.[290,1298,831,1748]" captionTargetPageId="5" captionText="Fig. 1. Exanthemachrysis fresneliae Véron sp. nov. (AC35), palmelloid and swimming cells, LM images. A. Benthic colonies of non-motile sister cells embedded in non-stratified M (black arrows) with storage granules of P (white arrows). B. Benthic non-motile cell embedded in non-stratified M (black arrow) with storage granule of P (white arrow), bilobed C and anterior bulging PY. C. Apical view (bottom right) and side view (up left) of 2 swimming cells with conspicuous S-shaped AF (black arrows) emerging almost mid-ventrally from a pit (white arrows). D. Side view of motile cells with bulging PY (black arrows). Scale bars: A = 5 µm; B = 1 µm; C–D = 2 µm." figureDoi="http://doi.org/10.5281/zenodo.7710260" httpUri="https://zenodo.org/record/7710260/files/figure.png" pageId="8" pageNumber="29">Figs 1A–B</figureCitation>
, 
<figureCitation id="13695C858467FFEDFF25FE6E6ACBFE48" box="[189,237,447,473]" captionStart-0="Fig" captionStart-1="Fig" captionStartId-0="6.[189,232,1822,1848]" captionStartId-1="7.[189,232,1857,1883]" captionTargetBox-0="[189,1398,568,1783]" captionTargetBox-1="[188,1397,738,1819]" captionTargetId-0="figure-82@6.[189,1398,568,1783]" captionTargetId-1="figure-97@7.[188,1397,738,1819]" captionTargetPageId-0="6" captionTargetPageId-1="7" captionText-0="Fig. 2. Exanthemachrysis fresneliae Véron sp. nov. (AC35), palmelloid sister cells, TEM image (Fix 1). Benthic colony of non-motile sister cells embedded in a common non-stratified M envelope (white stars); parietal C with helicoidal arrangement of the TH lamellae and a bulging PY near the F bases perpendicular to each other (white arrows), with the basal bodies connecting to the microtubular root (black arrows) running to the central area of the cell. Secretory vesicles from G (black star) ready to discharge content near the pit where the F emerge. Scale bar: 0.5 µm." captionText-1="Fig. 3. Exanthemachrysis fresneliae Véron sp. nov. (AC35), palmelloid cell, TEM image (Fix 1). Nonmotile benthic cell embedded in non-stratified M showing single parietal C with a bulging PY at its end. Row of few osmiophilic vesicles forming an E in C stroma located at the junction of TH and PY. Both F bases (white arrows) positioned in continuity with a notch in the nucleus facing the sub-ventral pit (black arrow). Scale bar:1 µm." figureDoi-0="http://doi.org/10.5281/zenodo.7710262" figureDoi-1="http://doi.org/10.5281/zenodo.7710264" httpUri-0="https://zenodo.org/record/7710262/files/figure.png" httpUri-1="https://zenodo.org/record/7710264/files/figure.png" pageId="8" pageNumber="29">2–3</figureCitation>
). Some cells can retain flagellar and haptonematal bases (
<figureCitation id="13695C858467FFEDFC09FE6E69FFFE48" box="[913,985,447,473]" captionStart="Fig" captionStartId="6.[189,232,1822,1848]" captionTargetBox="[189,1398,568,1783]" captionTargetId="figure-82@6.[189,1398,568,1783]" captionTargetPageId="6" captionText="Fig. 2. Exanthemachrysis fresneliae Véron sp. nov. (AC35), palmelloid sister cells, TEM image (Fix 1). Benthic colony of non-motile sister cells embedded in a common non-stratified M envelope (white stars); parietal C with helicoidal arrangement of the TH lamellae and a bulging PY near the F bases perpendicular to each other (white arrows), with the basal bodies connecting to the microtubular root (black arrows) running to the central area of the cell. Secretory vesicles from G (black star) ready to discharge content near the pit where the F emerge. Scale bar: 0.5 µm." figureDoi="http://doi.org/10.5281/zenodo.7710262" httpUri="https://zenodo.org/record/7710262/files/figure.png" pageId="8" pageNumber="29">Fig. 2</figureCitation>
).
</paragraph>
<paragraph id="8BED40008467FFEDFF25FDD06940FCEA" blockId="8.[189,1399,513,892]" pageId="8" pageNumber="29">
Non-phototactic swimming cells have a marked flattened oval shape (4.69 µm ± 0.61 × 2.76 µm ± 0.35, n = 50) with an anterolateral pit giving a slight bean shape to the side-view (
<figureCitation id="13695C858467FFEDFBE1FDF56EF2FDAF" box="[1145,1236,548,574]" captionStart="Fig" captionStartId="5.[189,232,1787,1813]" captionTargetBox="[290,1298,831,1748]" captionTargetId="figure-82@5.[290,1298,831,1748]" captionTargetPageId="5" captionText="Fig. 1. Exanthemachrysis fresneliae Véron sp. nov. (AC35), palmelloid and swimming cells, LM images. A. Benthic colonies of non-motile sister cells embedded in non-stratified M (black arrows) with storage granules of P (white arrows). B. Benthic non-motile cell embedded in non-stratified M (black arrow) with storage granule of P (white arrow), bilobed C and anterior bulging PY. C. Apical view (bottom right) and side view (up left) of 2 swimming cells with conspicuous S-shaped AF (black arrows) emerging almost mid-ventrally from a pit (white arrows). D. Side view of motile cells with bulging PY (black arrows). Scale bars: A = 5 µm; B = 1 µm; C–D = 2 µm." figureDoi="http://doi.org/10.5281/zenodo.7710260" httpUri="https://zenodo.org/record/7710260/files/figure.png" pageId="8" pageNumber="29">Fig. 1C</figureCitation>
). The parietal C is green-brownish (
<figureCitation id="13695C858467FFEDFE5AFD96683BFDF0" box="[450,541,583,609]" captionStart="Fig" captionStartId="5.[189,232,1787,1813]" captionTargetBox="[290,1298,831,1748]" captionTargetId="figure-82@5.[290,1298,831,1748]" captionTargetPageId="5" captionText="Fig. 1. Exanthemachrysis fresneliae Véron sp. nov. (AC35), palmelloid and swimming cells, LM images. A. Benthic colonies of non-motile sister cells embedded in non-stratified M (black arrows) with storage granules of P (white arrows). B. Benthic non-motile cell embedded in non-stratified M (black arrow) with storage granule of P (white arrow), bilobed C and anterior bulging PY. C. Apical view (bottom right) and side view (up left) of 2 swimming cells with conspicuous S-shaped AF (black arrows) emerging almost mid-ventrally from a pit (white arrows). D. Side view of motile cells with bulging PY (black arrows). Scale bars: A = 5 µm; B = 1 µm; C–D = 2 µm." figureDoi="http://doi.org/10.5281/zenodo.7710260" httpUri="https://zenodo.org/record/7710260/files/figure.png" pageId="8" pageNumber="29">Fig. 1C</figureCitation>
) with helicoidal and parallel arrangement of TH (
<figureCitation id="13695C858467FFEDFBE0FD966EE7FDF0" box="[1144,1217,583,609]" captionStart="Fig" captionStartId="6.[189,232,1822,1848]" captionTargetBox="[189,1398,568,1783]" captionTargetId="figure-82@6.[189,1398,568,1783]" captionTargetPageId="6" captionText="Fig. 2. Exanthemachrysis fresneliae Véron sp. nov. (AC35), palmelloid sister cells, TEM image (Fix 1). Benthic colony of non-motile sister cells embedded in a common non-stratified M envelope (white stars); parietal C with helicoidal arrangement of the TH lamellae and a bulging PY near the F bases perpendicular to each other (white arrows), with the basal bodies connecting to the microtubular root (black arrows) running to the central area of the cell. Secretory vesicles from G (black star) ready to discharge content near the pit where the F emerge. Scale bar: 0.5 µm." figureDoi="http://doi.org/10.5281/zenodo.7710262" httpUri="https://zenodo.org/record/7710262/files/figure.png" pageId="8" pageNumber="29">Fig. 2</figureCitation>
) and a bulging terminal PY (
<figureCitation id="13695C858467FFEDFEC3FDBB6BE2FD15" box="[347,452,618,644]" captionStart-0="Fig" captionStart-1="Fig" captionStart-2="Fig" captionStartId-0="5.[189,232,1787,1813]" captionStartId-1="6.[189,232,1822,1848]" captionStartId-2="7.[189,232,1857,1883]" captionTargetBox-0="[290,1298,831,1748]" captionTargetBox-1="[189,1398,568,1783]" captionTargetBox-2="[188,1397,738,1819]" captionTargetId-0="figure-82@5.[290,1298,831,1748]" captionTargetId-1="figure-82@6.[189,1398,568,1783]" captionTargetId-2="figure-97@7.[188,1397,738,1819]" captionTargetPageId-0="5" captionTargetPageId-1="6" captionTargetPageId-2="7" captionText-0="Fig. 1. Exanthemachrysis fresneliae Véron sp. nov. (AC35), palmelloid and swimming cells, LM images. A. Benthic colonies of non-motile sister cells embedded in non-stratified M (black arrows) with storage granules of P (white arrows). B. Benthic non-motile cell embedded in non-stratified M (black arrow) with storage granule of P (white arrow), bilobed C and anterior bulging PY. C. Apical view (bottom right) and side view (up left) of 2 swimming cells with conspicuous S-shaped AF (black arrows) emerging almost mid-ventrally from a pit (white arrows). D. Side view of motile cells with bulging PY (black arrows). Scale bars: A = 5 µm; B = 1 µm; C–D = 2 µm." captionText-1="Fig. 2. Exanthemachrysis fresneliae Véron sp. nov. (AC35), palmelloid sister cells, TEM image (Fix 1). Benthic colony of non-motile sister cells embedded in a common non-stratified M envelope (white stars); parietal C with helicoidal arrangement of the TH lamellae and a bulging PY near the F bases perpendicular to each other (white arrows), with the basal bodies connecting to the microtubular root (black arrows) running to the central area of the cell. Secretory vesicles from G (black star) ready to discharge content near the pit where the F emerge. Scale bar: 0.5 µm." captionText-2="Fig. 3. Exanthemachrysis fresneliae Véron sp. nov. (AC35), palmelloid cell, TEM image (Fix 1). Nonmotile benthic cell embedded in non-stratified M showing single parietal C with a bulging PY at its end. Row of few osmiophilic vesicles forming an E in C stroma located at the junction of TH and PY. Both F bases (white arrows) positioned in continuity with a notch in the nucleus facing the sub-ventral pit (black arrow). Scale bar:1 µm." figureDoi-0="http://doi.org/10.5281/zenodo.7710260" figureDoi-1="http://doi.org/10.5281/zenodo.7710262" figureDoi-2="http://doi.org/10.5281/zenodo.7710264" httpUri-0="https://zenodo.org/record/7710260/files/figure.png" httpUri-1="https://zenodo.org/record/7710262/files/figure.png" httpUri-2="https://zenodo.org/record/7710264/files/figure.png" pageId="8" pageNumber="29">Figs 1–3</figureCitation>
) adjacent to a deep pit (
<figureCitation id="13695C858467FFEDFD7AFDBB690FFD15" box="[738,809,618,644]" captionStart="Fig" captionStartId="7.[189,232,1857,1883]" captionTargetBox="[188,1397,738,1819]" captionTargetId="figure-97@7.[188,1397,738,1819]" captionTargetPageId="7" captionText="Fig. 3. Exanthemachrysis fresneliae Véron sp. nov. (AC35), palmelloid cell, TEM image (Fix 1). Nonmotile benthic cell embedded in non-stratified M showing single parietal C with a bulging PY at its end. Row of few osmiophilic vesicles forming an E in C stroma located at the junction of TH and PY. Both F bases (white arrows) positioned in continuity with a notch in the nucleus facing the sub-ventral pit (black arrow). Scale bar:1 µm." figureDoi="http://doi.org/10.5281/zenodo.7710264" httpUri="https://zenodo.org/record/7710264/files/figure.png" pageId="8" pageNumber="29">Fig. 3</figureCitation>
). A discreet E is located on the outer periphery of the C between the chloroplast stroma and the PY (
<figureCitation id="13695C858467FFEDFC8AFD5C697CFD39" box="[786,858,653,680]" captionStart="Fig" captionStartId="7.[189,232,1857,1883]" captionTargetBox="[188,1397,738,1819]" captionTargetId="figure-97@7.[188,1397,738,1819]" captionTargetPageId="7" captionText="Fig. 3. Exanthemachrysis fresneliae Véron sp. nov. (AC35), palmelloid cell, TEM image (Fix 1). Nonmotile benthic cell embedded in non-stratified M showing single parietal C with a bulging PY at its end. Row of few osmiophilic vesicles forming an E in C stroma located at the junction of TH and PY. Both F bases (white arrows) positioned in continuity with a notch in the nucleus facing the sub-ventral pit (black arrow). Scale bar:1 µm." figureDoi="http://doi.org/10.5281/zenodo.7710264" httpUri="https://zenodo.org/record/7710264/files/figure.png" pageId="8" pageNumber="29">Fig. 3</figureCitation>
). The AF (approximately 8–9 µm) is covered by a layer of ovoid KS (
<quantity id="4CAAEDE58467FFEDFE42FD606800FD5A" box="[474,550,689,715]" metricMagnitude="-8" metricUnit="m" metricValue="4.2" pageId="8" pageNumber="29" unit="nm" value="42.0">42 nm</quantity>
× 
<quantity id="4CAAEDE58467FFEDFDDEFD60688EFD5A" box="[582,680,689,716]" metricMagnitude="-8" metricUnit="m" metricValue="3.75" pageId="8" pageNumber="29" unit="nm" value="37.5">37.5 nm</quantity>
) produced in the Golgi body (
<figureCitation id="13695C858467FFEDFB96FD606E4EFD5D" box="[1038,1128,689,716]" captionStart="Fig" captionStartId="9.[189,232,1693,1719]" captionTargetBox="[189,1398,265,1656]" captionTargetId="figure-11@9.[189,1398,265,1656]" captionTargetPageId="9" captionText="Fig. 5. Exanthemachrysis fresneliae Véron sp. nov. (AC35), flagella, plasma membrane and knob-scales, TEM images (Fix 1). A. Longitudinal section of the pit area with 2 emerging F (white arrows = central microtubules) surrounded by a sheath of reticulum enclosing their bases. Plasma membrane (black arrow), underlined with endoplasmic reticulum (white stars), covered with KS. B. Details of the cell surface in the region of F emergence. Oblique sections of F bases with central microtubules (white arrow). Stalked ovoid KS partially covering the F bases and the plasma membrane. C. G with cisternae and secretory vesicles containing KS (white arrows). Scale bars: A–B = 50 nm; C = 0.5 µm." figureDoi="http://doi.org/10.5281/zenodo.7710268" httpUri="https://zenodo.org/record/7710268/files/figure.png" pageId="8" pageNumber="29">Fig. 5C</figureCitation>
) aligned in continuous helicoidal rows from the base (
<figureCitation id="13695C858467FFEDFDBDFD05684BFD7F" box="[549,621,724,750]" captionStart="Fig" captionStartId="8.[189,232,1823,1849]" captionTargetBox="[189,1398,1247,1786]" captionTargetId="figure-343@8.[189,1398,1247,1786]" captionTargetPageId="8" captionText="Fig. 4. Exanthemachrysis fresneliae Véron sp. nov. (AC35), flagellar apparatus of motile cells. A. SEM image of side view of swimming cell with a 3–4 µm long conspicuous sinusoidal S-shaped and slightly tapering AF emerging almost mid-ventrally and thickly covered by KS (white arrows). B–C. Negative staining image. B. Detail of the 3 appendices, the AF, the H and the PF composed of 2 parts of sub-equal lengths with a distal attenuation. C. Detail of the AF of a cell bearing ovoid KS (black arrows) down to the base, in regular helicoidal rows. Scale bars: A–B = 1 µm; C = 0.1 µm." figureDoi="http://doi.org/10.5281/zenodo.7710266" httpUri="https://zenodo.org/record/7710266/files/figure.png" pageId="8" pageNumber="29">Fig. 4</figureCitation>
). The PF (about 5–6 µm) is composed of two parts of almost equal length. The proximal part, comparable in diameter to the AF, tapers into a thinner distal part and the H, regular in diameter, measures about 2–3 µm. All three appendages emerge at the surface of the cell from the pit (
<figureCitation id="13695C858467FFEDFE8EFCEC6BBEFCC9" box="[278,408,829,856]" captionStart="Fig" captionStartId="9.[189,232,1693,1719]" captionTargetBox="[189,1398,265,1656]" captionTargetId="figure-11@9.[189,1398,265,1656]" captionTargetPageId="9" captionText="Fig. 5. Exanthemachrysis fresneliae Véron sp. nov. (AC35), flagella, plasma membrane and knob-scales, TEM images (Fix 1). A. Longitudinal section of the pit area with 2 emerging F (white arrows = central microtubules) surrounded by a sheath of reticulum enclosing their bases. Plasma membrane (black arrow), underlined with endoplasmic reticulum (white stars), covered with KS. B. Details of the cell surface in the region of F emergence. Oblique sections of F bases with central microtubules (white arrow). Stalked ovoid KS partially covering the F bases and the plasma membrane. C. G with cisternae and secretory vesicles containing KS (white arrows). Scale bars: A–B = 50 nm; C = 0.5 µm." figureDoi="http://doi.org/10.5281/zenodo.7710268" httpUri="https://zenodo.org/record/7710268/files/figure.png" pageId="8" pageNumber="29">Fig. 5A–B</figureCitation>
). Both the PF and H, as well as the cell membrane, are sporadically covered with KS identical (
<figureCitation id="13695C858467FFEDFEAAFCB06BAAFCED" box="[306,396,865,892]" captionStart="Fig" captionStartId="9.[189,232,1693,1719]" captionTargetBox="[189,1398,265,1656]" captionTargetId="figure-11@9.[189,1398,265,1656]" captionTargetPageId="9" captionText="Fig. 5. Exanthemachrysis fresneliae Véron sp. nov. (AC35), flagella, plasma membrane and knob-scales, TEM images (Fix 1). A. Longitudinal section of the pit area with 2 emerging F (white arrows = central microtubules) surrounded by a sheath of reticulum enclosing their bases. Plasma membrane (black arrow), underlined with endoplasmic reticulum (white stars), covered with KS. B. Details of the cell surface in the region of F emergence. Oblique sections of F bases with central microtubules (white arrow). Stalked ovoid KS partially covering the F bases and the plasma membrane. C. G with cisternae and secretory vesicles containing KS (white arrows). Scale bars: A–B = 50 nm; C = 0.5 µm." figureDoi="http://doi.org/10.5281/zenodo.7710268" httpUri="https://zenodo.org/record/7710268/files/figure.png" pageId="8" pageNumber="29">Fig. 5C</figureCitation>
) to those present on the AF (
<figureCitation id="13695C858467FFEDFD47FCB0697FFCEA" box="[735,857,865,891]" captionStart="Fig" captionStartId="8.[189,232,1823,1849]" captionTargetBox="[189,1398,1247,1786]" captionTargetId="figure-343@8.[189,1398,1247,1786]" captionTargetPageId="8" captionText="Fig. 4. Exanthemachrysis fresneliae Véron sp. nov. (AC35), flagellar apparatus of motile cells. A. SEM image of side view of swimming cell with a 3–4 µm long conspicuous sinusoidal S-shaped and slightly tapering AF emerging almost mid-ventrally and thickly covered by KS (white arrows). B–C. Negative staining image. B. Detail of the 3 appendices, the AF, the H and the PF composed of 2 parts of sub-equal lengths with a distal attenuation. C. Detail of the AF of a cell bearing ovoid KS (black arrows) down to the base, in regular helicoidal rows. Scale bars: A–B = 1 µm; C = 0.1 µm." figureDoi="http://doi.org/10.5281/zenodo.7710266" httpUri="https://zenodo.org/record/7710266/files/figure.png" pageId="8" pageNumber="29">Fig. 4A,C</figureCitation>
).
</paragraph>
<paragraph id="8BED40008467FFEDFF25FC736E77FC2C" blockId="8.[189,1105,930,957]" box="[189,1105,930,957]" pageId="8" pageNumber="29">
<emphasis id="B9269C128467FFEDFF25FC736E77FC2C" bold="true" box="[189,1105,930,957]" pageId="8" pageNumber="29">
Taxonomic outcome: a revised description of the genus 
<taxonomicName id="4C523B838467FFEDFCE8FC736E77FC2C" box="[880,1105,930,957]" class="Pavlovophyceae" family="Pavlovaceae" genus="Exanthemachrysis" kingdom="Chromista" order="Pavlovales" pageId="8" pageNumber="29" phylum="Haptophyta" rank="genus">
<emphasis id="B9269C128467FFEDFCE8FC736E77FC2C" bold="true" box="[880,1105,930,957]" italics="true" pageId="8" pageNumber="29">Exanthemachrysis</emphasis>
</taxonomicName>
</emphasis>
</paragraph>
<paragraph id="8BED40008467FFEDFF25FC006946FBC5" blockId="8.[189,1399,977,1108]" pageId="8" pageNumber="29">
Due to the description of a second species in the genus 
<taxonomicName id="4C523B838467FFEDFCC7FC006E11FC7A" box="[863,1079,977,1003]" class="Pavlovophyceae" family="Pavlovaceae" genus="Exanthemachrysis" kingdom="Chromista" order="Pavlovales" pageId="8" pageNumber="29" phylum="Haptophyta" rank="genus">
<emphasis id="B9269C128467FFEDFCC7FC006E11FC7A" box="[863,1079,977,1003]" italics="true" pageId="8" pageNumber="29">Exanthemachrysis</emphasis>
</taxonomicName>
, a revision of the genus is necessary. The description based on 
<taxonomicName id="4C523B838467FFEDFDF4FC246E7EFB9F" authority="(Lepailleur) Bendif &amp; Veron" authorityName="Bendif &amp; Veron" baseAuthorityName="Lepailleur" box="[620,1112,1012,1038]" class="Pavlovophyceae" family="Pavlovaceae" genus="Exanthemachrysis" kingdom="Chromista" order="Pavlovales" pageId="8" pageNumber="29" phylum="Haptophyta" rank="species" species="gayraliae">
<emphasis id="B9269C128467FFEDFDF4FC2468DBFB9F" box="[620,765,1012,1038]" italics="true" pageId="8" pageNumber="29">E. gayraliae</emphasis>
(Lepailleur) Bendif &amp; Véron
</taxonomicName>
has become too general and can now be emended following the description of 
<taxonomicName id="4C523B838467FFEDFCDAFBC969F0FBA0" authority="Véron &amp; Rougier &amp; Taylor &amp; Goux, 2023" authorityName="Véron &amp; Rougier &amp; Taylor &amp; Goux" authorityYear="2023" box="[834,982,1047,1073]" class="Pavlovophyceae" family="Pavlovaceae" genus="Exanthemachrysis" kingdom="Chromista" order="Pavlovales" pageId="8" pageNumber="29" phylum="Haptophyta" rank="species" species="fresneliae" status="sp. nov.">
<emphasis id="B9269C128467FFEDFCDAFBC969F0FBA0" box="[834,982,1047,1073]" italics="true" pageId="8" pageNumber="29">E. fresneliae</emphasis>
</taxonomicName>
<taxonomicNameLabel id="A21521698467FFEDFC45FBC96E1EFBA3" box="[989,1080,1048,1074]" pageId="8" pageNumber="29" rank="species">sp. nov.</taxonomicNameLabel>
and other 
<taxonomicName id="4C523B838467FFEDFB2EFBC66F50FBA0" box="[1206,1398,1047,1073]" class="Pavlovophyceae" kingdom="Chromista" pageId="8" pageNumber="29" phylum="Haptophyta" rank="class">Pavlovophyceae</taxonomicName>
described since, providing information unknown in 1970.
</paragraph>
</subSubSection>
</treatment>
</document>