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109 lines
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<document id="7B11DDCCD98E99572114D707F3CBD927" ID-DOI="10.1016/j.phytochem.2018.11.006" ID-ISSN="1873-3700" ID-Zenodo-Dep="10481556" IM.bibliography_approvedBy="karina" IM.illustrations_approvedBy="felipe" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.taxonomicNames_approvedBy="karina" IM.treatments_approvedBy="karina" checkinTime="1704926936264" checkinUser="felipe" docAuthor="Sui, Ziwei, Luo, Jun, Yao, Ruolan, Huang, Chuanlong, Zhao, Yucheng & Kong, Lingyi" docDate="2019" docId="2812FE14FA2AAC2FFCDBFB69FC7AF9BC" docLanguage="en" docName="Phytochemistry.158.35-45.pdf" docOrigin="Phytochemistry 158" docSource="http://dx.doi.org/10.1016/j.phytochem.2018.11.006" docStyle="DocumentStyle:9E596C34F4E94307D29315B03ACE1007.6:Phytochemistry.2014-2019.journal_article" docStyleId="9E596C34F4E94307D29315B03ACE1007" docStyleName="Phytochemistry.2014-2019.journal_article" docStyleVersion="6" docTitle="Peucedanum praeruptorum Dunn" docType="treatment" docVersion="6" lastPageNumber="36" masterDocId="D42B866CFA2BAC2EFFE9FFBEFFA4FFE9" masterDocTitle="Functional characterization and correlation analysis of phenylalanine ammonia-lyase (PAL) in coumarin biosynthesis from Peucedanum praeruptorum Dunn" masterLastPageNumber="45" masterPageNumber="35" pageNumber="36" updateTime="1706147393680" updateUser="karina">
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<mods:titleInfo id="CB33F3F8D88C1797944667993B08992C">
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<mods:title id="3C0C3AA88B255F09794A5C12E71DF5BC">Functional characterization and correlation analysis of phenylalanine ammonia-lyase (PAL) in coumarin biosynthesis from Peucedanum praeruptorum Dunn</mods:title>
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<mods:namePart id="BE49198477B00FBFD1FAB7A32C8DCFB5">Sui, Ziwei</mods:namePart>
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<mods:namePart id="B502FCA8F68129A218CB760CF6A63690">Luo, Jun</mods:namePart>
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<mods:namePart id="87777E2B01225D37A9DE10651E5C8160">Yao, Ruolan</mods:namePart>
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<mods:namePart id="1F0739956CC00821AD0486DF5092BB21">Huang, Chuanlong</mods:namePart>
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<mods:namePart id="2636D2907C8894D6F3EBE80824990416">Zhao, Yucheng</mods:namePart>
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<mods:namePart id="38CA988E4D0A3F5BD95545159BB4A820">Kong, Lingyi</mods:namePart>
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<mods:title id="84D74775989BBEFCA147932F899A6A1C">Phytochemistry</mods:title>
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<mods:date id="7F04ECE3D1DF79B2D49441CA73A63C51">2019</mods:date>
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<mods:number id="5CD3654754B75508DA3DF9BB65F78439">2019-02-28</mods:number>
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<mods:identifier id="84D32B8BC290BBF3394ECF41AC9320BA" type="DOI">10.1016/j.phytochem.2018.11.006</mods:identifier>
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<mods:identifier id="B5FEEE330E036FED58576C58D2C7E495" type="ISSN">1873-3700</mods:identifier>
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<treatment id="2812FE14FA2AAC2FFCDBFB69FC7AF9BC" LSID="urn:lsid:plazi:treatment:2812FE14FA2AAC2FFCDBFB69FC7AF9BC" httpUri="http://treatment.plazi.org/id/2812FE14FA2AAC2FFCDBFB69FC7AF9BC" lastPageNumber="36" pageId="1" pageNumber="36">
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<subSubSection id="E8A11C89FA2AAC2FFCDBFB69FADAFB03" box="[818,1406,1239,1258]" pageId="1" pageNumber="36" type="nomenclature">
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<paragraph id="A0044F02FA2AAC2FFCDBFB69FADAFB03" blockId="1.[818,1406,1239,1258]" box="[818,1406,1239,1258]" pageId="1" pageNumber="36">
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<heading id="FB4CF86EFA2AAC2FFCDBFB69FADAFB03" bold="true" box="[818,1406,1239,1258]" fontSize="36" level="1" pageId="1" pageNumber="36" reason="1">
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<emphasis id="92CF9310FA2AAC2FFCDBFB69FADAFB03" bold="true" box="[818,1406,1239,1258]" italics="true" pageId="1" pageNumber="36">
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2.1. Identification of the cDNA encoding PAL in
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<taxonomicName id="67BB3481FA2AAC2FFB05FB69FADAFB03" ID-CoL="4FFHM" authority="Dunn" authorityName="Dunn" box="[1260,1406,1239,1258]" class="Magnoliopsida" family="Apiaceae" genus="Peucedanum" kingdom="Plantae" order="Apiales" pageId="1" pageNumber="36" phylum="Tracheophyta" rank="species" species="praeruptorum">P. praeruptorum</taxonomicName>
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</emphasis>
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<subSubSection id="E8A11C89FA2AAC2FFCBAFAB1FC7AF9BC" pageId="1" pageNumber="36" type="description">
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<paragraph id="A0044F02FA2AAC2FFCBAFAB1FC7AF9BC" blockId="1.[818,1488,1295,1621]" pageId="1" pageNumber="36">
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Using PAL sequences reported previously and our transcriptome database of
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<taxonomicName id="67BB3481FA2AAC2FFC40FA95FB9AFAD7" box="[937,1086,1323,1342]" class="Magnoliopsida" family="Apiaceae" genus="Peucedanum" kingdom="Plantae" order="Apiales" pageId="1" pageNumber="36" phylum="Tracheophyta" rank="species" species="praeruptorum">
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<emphasis id="92CF9310FA2AAC2FFC40FA95FB9AFAD7" bold="true" box="[937,1086,1323,1342]" italics="true" pageId="1" pageNumber="36">P. praeruptorum</emphasis>
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</taxonomicName>
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(accession no. SRX997427) (
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<bibRefCitation id="C42A32F3FA2AAC2FFAB6FA95FCC6FAB3" author="Zhao, Y. C. & Liu, T. T. & Luo, J. & Zhang, Q. & Xu, S. & Han, C. & Xu, J. F. & Chen, M. H. & Chen, Y. J. & Kong, L. Y." pageId="1" pageNumber="36" pagination="996" refId="ref11442" refString="Zhao, Y. C., Liu, T. T., Luo, J., Zhang, Q., Xu, S., Han, C., Xu, J. F., Chen, M. H., Chen, Y. J., Kong, L. Y., 2015. Integration of a decrescent transcriptome and metabolomics dataset of Peucedanum praeruptorum to investigate the CYP 450 and MDR genes involved in coumarins biosynthesis and transport. Front. Plant Sci. 6, 996. https: // doi. org / 10. 3389 / fpls. 2015.00996." type="journal article" year="2015">Zhao et al., 2015</bibRefCitation>
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), a BLAST search was conducted. According to the
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<emphasis id="92CF9310FA2AAC2FFA84FAF9FADDFAB3" bold="true" box="[1389,1401,1351,1370]" italics="true" pageId="1" pageNumber="36">E</emphasis>
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-value of alignments, only one
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<emphasis id="92CF9310FA2AAC2FFC15FADDFB85FA9F" bold="true" box="[1020,1057,1379,1398]" italics="true" pageId="1" pageNumber="36">PAL</emphasis>
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was identified and cloned. This cDNA, which encodes a 719 amino acid residue protein (Table S1), was designated
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<emphasis id="92CF9310FA2AAC2FFCDBFA24FCCAFA44" bold="true" box="[818,878,1434,1453]" italics="true" pageId="1" pageNumber="36">PpPAL</emphasis>
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. According to bioinformatics, PpPAL is most closely related to PAL of
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<taxonomicName id="67BB3481FA2AAC2FFC90FA08FBC2FA20" authority="(AsPAL)" baseAuthorityName="AsPAL" box="[889,1126,1462,1481]" class="Magnoliopsida" family="Apiaceae" genus="Angelica" kingdom="Plantae" order="Apiales" pageId="1" pageNumber="36" phylum="Tracheophyta" rank="species" species="sinensis">
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<emphasis id="92CF9310FA2AAC2FFC90FA08FBABFA20" bold="true" box="[889,1039,1462,1481]" italics="true" pageId="1" pageNumber="36">Angelica sinensis</emphasis>
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(AsPAL)
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</taxonomicName>
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, which also belongs to
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<taxonomicName id="67BB3481FA2AAC2FFAA1FA08FA39FA20" box="[1352,1437,1462,1481]" class="Magnoliopsida" family="Apiaceae" kingdom="Plantae" order="Apiales" pageId="1" pageNumber="36" phylum="Tracheophyta" rank="family">Apiaceae</taxonomicName>
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(
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<figureCitation id="38805387FA2AAC2FFA45FA08FCECFA0C" captionStart="Fig" captionStartId="2.[100,130,1913,1930]" captionTargetBox="[128,1458,153,1890]" captionTargetId="figure-0@2.[127,1459,152,1891]" captionTargetPageId="2" captionText="Fig. 2. The content changes of Praeruptorin A, Praeruptorin B and Imperatorin. A. The content of three coumarin compounds in different tissues (Root, Stem and Leaf). B. The content changes of three coumarin compounds under MeJA elicitation. C. The content changes of three coumarin compounds under H2O2 treatment. D. The content changes of three coumarin compounds under UV elicitation. E. The content changes of three coumarin compounds under cold treatment. F. The content changes of three coumarin compounds under heat treatment. Values represent means ± SD (n = 3)." figureDoi="http://doi.org/10.5281/zenodo.10481560" httpUri="https://zenodo.org/record/10481560/files/figure.png" pageId="1" pageNumber="36">Fig. S2</figureCitation>
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). The amino acid sequences of PpPAL also contain a highly conserved MIO group, which is formed by Ala-Ser-Gly residues (
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<figureCitation id="38805387FA2AAC2FFA96FA50FA65F9E8" box="[1407,1473,1518,1537]" captionStart="Fig" captionStartId="4.[100,130,852,869]" captionTargetBox="[264,1324,153,828]" captionTargetId="figure-0@4.[263,1325,152,829]" captionTargetPageId="4" captionText="Fig. 3. Subcellular localization of PpPAL in Arabidopsis protoplasts. Green panels show GFP fluorescence, red panels show mCherry fluorescence and chloroplast autofluorescence is shown in blue panels. Merged panels represent combined fluorescence from GFP, mCherry and chloroplasts. Arabidopsis protoplasts containing GFP were used as a blank control and AtGAPC1-mCherry was used as a positive control for cytosol localization. Bars = 20 μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)" figureDoi="http://doi.org/10.5281/zenodo.10481562" httpUri="https://zenodo.org/record/10481562/files/figure.png" pageId="1" pageNumber="36">Fig. S3</figureCitation>
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). The high sequence similarity revealed that they might display the same enzymatic functions such as substrate specificity, optimal pH and catalytic mechanism.
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</paragraph>
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</subSubSection>
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</treatment>
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