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<document ID-DOI="http://dx.doi.org/10.3897/BDJ.9.e60604" ID-PMC="PMC7809010" ID-Pensoft-Pub="1314-2828-9-e60604" ID-Pensoft-UUID="529C29AC5B6A5436A869DD8753970275" ID-PubMed="33510578" ModsDocID="1314-2828-9-e60604" checkinTime="1610047052867" checkinUser="pensoft" docAuthor="Meng, Chao-Rong, Zhang, Qian, Yang, Zai-Fu, Geng, Kun, Zeng, Xiang-Yu, Thilini Chethana, K. W. &amp; Wang, Yong" docDate="2021" docId="36593BB5E94055FFA752C47223FD800D" docLanguage="en" docName="BiodivDatJour 9: e60604" docOrigin="Biodiversity Data Journal 9" docPubDate="2021-01-07" docSource="http://dx.doi.org/10.3897/BDJ.9.e60604" docTitle="Lasiodiplodia syzygii C. R. Meng, Qian Zhang &amp; Yong Wang bis 2021, sp. nov." docType="treatment" docVersion="3" id="529C29AC5B6A5436A869DD8753970275" lastPageNumber="60604" masterDocId="529C29AC5B6A5436A869DD8753970275" masterDocTitle="Lasiodiplodia syzygii sp. nov. (Botryosphaeriaceae) causing post-harvest water-soaked brown lesions on Syzygium samarangense in Chiang Rai, Thailand" masterLastPageNumber="60604" masterPageNumber="60604" pageNumber="60604" updateTime="1668128625330" updateUser="ExternalLinkService">
<mods:mods xmlns:mods="http://www.loc.gov/mods/v3">
<mods:titleInfo>
<mods:title>Lasiodiplodia syzygii sp. nov. (Botryosphaeriaceae) causing post-harvest water-soaked brown lesions on Syzygium samarangense in Chiang Rai, Thailand</mods:title>
</mods:titleInfo>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Meng, Chao-Rong</mods:namePart>
<mods:nameIdentifier type="ORCID">https://orcid.org/0000-0001-5549-1028</mods:nameIdentifier>
<mods:affiliation>Department of Plant Pathology, Agricultural College, Guizhou University, Guiyang, China</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Zhang, Qian</mods:namePart>
<mods:affiliation>Department of Plant Pathology, Agricultural College, Guizhou University, Guiyang, China</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Yang, Zai-Fu</mods:namePart>
<mods:affiliation>Department of Plant Pathology, Agricultural College, Guizhou University, Guiyang, China</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Geng, Kun</mods:namePart>
<mods:affiliation>Guiyang plant protection and inspection station, Guiyang, China</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Zeng, Xiang-Yu</mods:namePart>
<mods:affiliation>Department of Plant Pathology, Agricultural College, Guizhou University, Guiyang, China</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Thilini Chethana, K. W.</mods:namePart>
<mods:nameIdentifier type="ORCID">https://orcid.org/0000-0002-5816-9269</mods:nameIdentifier>
<mods:affiliation>Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai, Thailand &amp; School of Science, Mae Fah Luang University, Chiang Rai, Thailand</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Wang, Yong</mods:namePart>
<mods:affiliation>Department of Plant Pathology, Agricultural College, Guizhou University, Guiyang, China</mods:affiliation>
<mods:nameIdentifier type="email">yongwangbis@aliyun.com</mods:nameIdentifier>
</mods:name>
<mods:typeOfResource>text</mods:typeOfResource>
<mods:relatedItem type="host">
<mods:titleInfo>
<mods:title>Biodiversity Data Journal</mods:title>
</mods:titleInfo>
<mods:part>
<mods:date>2021</mods:date>
<mods:detail type="pubDate">
<mods:number>2021-01-07</mods:number>
</mods:detail>
<mods:detail type="volume">
<mods:number>9</mods:number>
</mods:detail>
<mods:extent unit="page">
<mods:start>60604</mods:start>
<mods:end>60604</mods:end>
</mods:extent>
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<mods:location>
<mods:url>http://dx.doi.org/10.3897/BDJ.9.e60604</mods:url>
</mods:location>
<mods:classification>journal article</mods:classification>
<mods:identifier type="DOI">http://dx.doi.org/10.3897/BDJ.9.e60604</mods:identifier>
<mods:identifier type="Pensoft-Pub">1314-2828-9-e60604</mods:identifier>
<mods:identifier type="Pensoft-UUID">529C29AC5B6A5436A869DD8753970275</mods:identifier>
</mods:mods>
<treatment LSID="urn:lsid:plazi:treatment:36593BB5E94055FFA752C47223FD800D" httpUri="http://treatment.plazi.org/id/36593BB5E94055FFA752C47223FD800D" lastPageNumber="60604" pageId="0" pageNumber="60604">
<subSubSection pageId="0" pageNumber="60604" type="nomenclature">
<paragraph pageId="0" pageNumber="60604">
<taxonomicName LSID="36593BB5-E940-55FF-A752-C47223FD800D" authority="C. R. Meng, Qian Zhang &amp; Yong Wang bis" authorityName="C. R. Meng, Qian Zhang &amp; Yong Wang bis" authorityYear="2021" class="Dothideomycetes" family="Botryosphaeriaceae" genus="Lasiodiplodia" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Lasiodiplodia syzygii" order="Botryosphaeriales" pageId="0" pageNumber="60604" phylum="Ascomycota" rank="species" species="syzygii" status="sp. nov.">Lasiodiplodia syzygii C.R. Meng, Qian Zhang &amp; Yong Wang bis</taxonomicName>
<taxonomicNameLabel pageId="0" pageNumber="60604">sp. nov.</taxonomicNameLabel>
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="60604" type="materials_examined">
<paragraph pageId="0" pageNumber="60604">Materials</paragraph>
<paragraph pageId="0" pageNumber="60604">
<materialsCitation collectionCode="HGUP" collectorName="Wang Yong" country="Thailand" location="Thailand" pageId="0" pageNumber="60604" specimenCode="HGUP 9719" specimenCount="1" typeStatus="Holotype">
<emphasis bold="true" pageId="0" pageNumber="60604">Type status:</emphasis>
<typeStatus pageId="0" pageNumber="60604">Holotype</typeStatus>
.
<emphasis bold="true" pageId="0" pageNumber="60604">Occurrence:</emphasis>
catalogNumber:
<specimenCode pageId="0" pageNumber="60604">HGUP 9719</specimenCode>
; recordedBy:
<collectorName pageId="0" pageNumber="60604">Wang Yong</collectorName>
;
<emphasis bold="true" pageId="0" pageNumber="60604">Taxon:</emphasis>
scientificName: Lasiodiplodiasyzygii; kingdom: Fungi; class: Dothideomycetes; order: Botryosphaeriales; family: Botryosphaeriaceae; genus: Lasiodiplodia;
<emphasis bold="true" pageId="0" pageNumber="60604">Location:</emphasis>
country:
<collectingCountry pageId="0" pageNumber="60604">Thailand</collectingCountry>
; stateProvince: Chiang Rai;
<emphasis bold="true" pageId="0" pageNumber="60604">Identification:</emphasis>
identifiedBy:
<determinerName pageId="0" pageNumber="60604">Chao-Rong Meng</determinerName>
; dateIdentified: 2020;
<emphasis bold="true" pageId="0" pageNumber="60604">Record Level:</emphasis>
type: ex-type living culture GUCC 9719.1; MFLU 19-0565, isotype, isotype living culture MFLUCC 19-0257.
</materialsCitation>
<materialsCitation collectionCode="HGUP" collectorName="Wang Yong" country="China" location="China" pageId="0" pageNumber="60604" specimenCode="HGUP 9720 and HGUP 9721" specimenCount="1" typeStatus="Other material">
<emphasis bold="true" pageId="0" pageNumber="60604">Type status:</emphasis>
<typeStatus pageId="0" pageNumber="60604">Other material</typeStatus>
.
<emphasis bold="true" pageId="0" pageNumber="60604">Occurrence:</emphasis>
catalogNumber:
<specimenCode pageId="0" pageNumber="60604">HGUP 9720 and HGUP 9721</specimenCode>
; recordedBy:
<collectorName pageId="0" pageNumber="60604">Wang Yong</collectorName>
;
<emphasis bold="true" pageId="0" pageNumber="60604">Taxon:</emphasis>
scientificName: Lasiodiplodiasyzygii; kingdom: Fungi; class: Dothideomycetes; order: Botryosphaeriales; family: Botryosphaeriaceae; genus: Lasiodiplodia;
<emphasis bold="true" pageId="0" pageNumber="60604">Location:</emphasis>
country:
<collectingCountry pageId="0" pageNumber="60604">China</collectingCountry>
; stateProvince: Guiyang;
<emphasis bold="true" pageId="0" pageNumber="60604">Identification:</emphasis>
identifiedBy:
<determinerName pageId="0" pageNumber="60604">Chao-Rong Meng</determinerName>
; dateIdentified: 2020;
<emphasis bold="true" pageId="0" pageNumber="60604">Record Level:</emphasis>
type: living cultures GUCC 9719.2, GUCC 9719.3 and GUCC 9719.4
</materialsCitation>
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="60604" type="description">
<paragraph pageId="0" pageNumber="60604">Description</paragraph>
<paragraph pageId="0" pageNumber="60604">
<taxonomicName genus="Pathogenic" lsidName="Pathogenic" pageId="0" pageNumber="60604" rank="genus">
<emphasis italics="true" pageId="0" pageNumber="60604">Pathogenic</emphasis>
</taxonomicName>
on
<taxonomicName class="Dothideomycetes" family="Myrtaceae" genus="Syzygium" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Syzygium samarangense" order="Myrtales" pageId="0" pageNumber="60604" phylum="Ascomycota" rank="species" species="samarangense">
<emphasis italics="true" pageId="0" pageNumber="60604">Syzygium samarangense</emphasis>
</taxonomicName>
.
<emphasis bold="true" pageId="0" pageNumber="60604">Sexual morph</emphasis>
: Undetermined.
<emphasis bold="true" pageId="0" pageNumber="60604">Asexual morph</emphasis>
(Fig.
<figureCitation captionStart="Figure 2" captionStartId="F6360986" captionText="Figure 2. Lasiodiplodia syzygii (MFLUCC 19 - 0257). a. infected fruit; b, c. Conidiomata on the host; d. Section through a conidioma; e. Conidia developing amongst paraphyses; f-h. Conidia formed on conidiogenous cells; i-m. Immature conidia; n-o. Colonies on PDA culture; n. From above; o. From below. Scale bars: b = 300 μm, c = 140 μm, d = 50 μm, e = 20 μm, f-m = 10 μm." figureDoi="10.3897/BDJ.9.e60604.figure2" httpUri="https://binary.pensoft.net/fig/472377" pageId="0" pageNumber="60604">2</figureCitation>
):
<emphasis italics="true" pageId="0" pageNumber="60604">Conidiomata</emphasis>
up to 2 mm diam., pycnidial, covered with hyphae, black, globose, ostiolate, solitary, separate, uniloculate, immersed to semi-immersed.
<taxonomicName genus="Conidiomatal" lsidName="Conidiomatal wall" pageId="0" pageNumber="60604" rank="species" species="wall">
<emphasis italics="true" pageId="0" pageNumber="60604">Conidiomatal wall</emphasis>
</taxonomicName>
composed of thick-walled, dark brown cells of
<emphasis italics="true" pageId="0" pageNumber="60604">textura angularis</emphasis>
, becoming thin-walled and hyaline towards the inner region.
<emphasis italics="true" pageId="0" pageNumber="60604">Paraphyses</emphasis>
cylindrical, aseptate, hyaline.
<emphasis italics="true" pageId="0" pageNumber="60604">Conidiophores</emphasis>
reduced to conidiogenous cells.
<emphasis italics="true" pageId="0" pageNumber="60604">Conidiogenous cells</emphasis>
10-14.5
<normalizedToken originalValue="×">x</normalizedToken>
3.5-4.5
<normalizedToken originalValue="μm">μm</normalizedToken>
(average = 11
<normalizedToken originalValue="×">x</normalizedToken>
3.7
<normalizedToken originalValue="μm">μm</normalizedToken>
, n = 20), hyaline, smooth, holoblastic forming conidia at their tips.
<emphasis italics="true" pageId="0" pageNumber="60604">Conidia</emphasis>
thick-walled,
<taxonomicName genus="Conidiomatal" lsidName="Conidiomatal wall" pageId="0" pageNumber="60604" rank="species" species="wall">Conidiomatal wall</taxonomicName>
up to 1
<normalizedToken originalValue="μm">μm</normalizedToken>
wide, ovoid with both ends rounded, hyaline and remaining so for a long time, becoming pale brown with obsolete striations and occasionally with 1-septate after discharging from the conidioma, (27-)30-32(-36)
<normalizedToken originalValue="×">x</normalizedToken>
(13-)15-17(-20)
<normalizedToken originalValue="μm">μm</normalizedToken>
(average = 31.3
<normalizedToken originalValue="×">x</normalizedToken>
16.4
<normalizedToken originalValue="μm">μm</normalizedToken>
, n = 50), L/W = 1.9.
</paragraph>
<paragraph pageId="0" pageNumber="60604">
<emphasis italics="true" pageId="0" pageNumber="60604">Culture characteristics</emphasis>
: Conidia germinate on PDA within 24 hours at room temperature (25-30°C) with germ tubes produced from both ends of the conidia. Colonies with white fluffy mycelium on PDA, after 7 days become olivaceous-grey at the centre, white at the edge, raised, fluffy, dense filamentous.
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="60604" type="notes">
<paragraph pageId="0" pageNumber="60604">Notes</paragraph>
<paragraph pageId="0" pageNumber="60604">
<taxonomicName authorityName="C. R. Meng, Qian Zhang &amp; Yong Wang bis" authorityYear="2021" class="Dothideomycetes" family="Botryosphaeriaceae" genus="Lasiodiplodia" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Lasiodiplodia syzygii" order="Botryosphaeriales" pageId="0" pageNumber="60604" phylum="Ascomycota" rank="species" species="syzygii">
<emphasis italics="true" pageId="0" pageNumber="60604">Lasiodiplodia syzygii</emphasis>
</taxonomicName>
strains are closely related to
<taxonomicName lsidName="L. rubropurpurea" pageId="0" pageNumber="60604" rank="species" species="rubropurpurea">
<emphasis italics="true" pageId="0" pageNumber="60604">L. rubropurpurea</emphasis>
</taxonomicName>
, but formed a distinct, well-supported clade in the phylogenetic analyses. Base-pairs comparisons between
<taxonomicName lsidName="L. syzygii" pageId="0" pageNumber="60604" rank="species" species="syzygii">
<emphasis italics="true" pageId="0" pageNumber="60604">L. syzygii</emphasis>
</taxonomicName>
ex-type strain (GUCC 9719.1) and ex-type strain of
<taxonomicName lsidName="L. rubropurpurea" pageId="0" pageNumber="60604" rank="species" species="rubropurpurea">
<emphasis italics="true" pageId="0" pageNumber="60604">L. rubropurpurea</emphasis>
</taxonomicName>
(WAC 12535) found seven base differences (1.3%) in ITS region and five differences (0.6%) on LSU, but nine differences (2.1%) in
<emphasis italics="true" pageId="0" pageNumber="60604">tub2</emphasis>
and 34 in TEF1-a (10.4%) (Table
<tableCitation captionStart="Table 2" captionStartId="T6360993" captionText="Table 2. DNA base pair differences between Lasiodiplodia syzygii and L. rubropurpurea in four separate loci. T = ex-type" httpUri="http://table.plazi.org/id/7C0323EDC7010F684B217A8C8191C2FF" pageId="0" pageNumber="60604" tableUuid="7C0323EDC7010F684B217A8C8191C2FF">2</tableCitation>
).
<taxonomicName authorityName="C. R. Meng, Qian Zhang &amp; Yong Wang bis" authorityYear="2021" class="Dothideomycetes" family="Botryosphaeriaceae" genus="Lasiodiplodia" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Lasiodiplodia syzygii" order="Botryosphaeriales" pageId="0" pageNumber="60604" phylum="Ascomycota" rank="species" species="syzygii">
<emphasis italics="true" pageId="0" pageNumber="60604">Lasiodiplodia syzygii</emphasis>
</taxonomicName>
produced larger pycnidia (up to 2 mm) and larger conidiogenous cells (10-14.5
<normalizedToken originalValue="×">x</normalizedToken>
3.5-4.5
<normalizedToken originalValue="μm">μm</normalizedToken>
) than
<taxonomicName lsidName="L. rubropurpurea" pageId="0" pageNumber="60604" rank="species" species="rubropurpurea">
<emphasis italics="true" pageId="0" pageNumber="60604">L. rubropurpurea</emphasis>
</taxonomicName>
(0.5-1.5 mm and 7-13
<normalizedToken originalValue="×">x</normalizedToken>
3-5
<normalizedToken originalValue="μm">μm</normalizedToken>
) (
<bibRefCitation author="Burgess, T I" journalOrPublisher="Mycologia" pageId="0" pageNumber="60604" pagination="423 - 435" refId="B6361247" refString="Burgess, T I, Barber, P. A, Mohali, Sari, Pegg, Geoff, de Beer, Wilhelm, Wingfield, M J, 2006. Three new Lasiodiplodia spp. from the tropics, recognized based on DNA sequence comparisons and morphology. Mycologia 98 (3): 423 - 435" title="Three new Lasiodiplodia spp. from the tropics, recognized based on DNA sequence comparisons and morphology" volume="98" year="2006">Burgess et al. 2006</bibRefCitation>
).
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="60604" type="etymology">
<paragraph pageId="0" pageNumber="60604">Etymology</paragraph>
<paragraph pageId="0" pageNumber="60604">In reference to the host from which the fungus was first isolated.</paragraph>
</subSubSection>
</treatment>
</document>