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<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.39.26914" ID-GBIF-Dataset="eda7a41a-f75a-4daa-ad76-1963372cba89" ID-PMC="PMC6160862" ID-Pensoft-Pub="1314-4049-39-97" ID-PubMed="30271260" ModsDocAuthor="" ModsDocDate="2018" ModsDocID="1314-4049-39-97" ModsDocOrigin="MycoKeys 39" ModsDocTitle="High diversity of Diaporthe species associated with dieback diseases in China, with twelve new species described" checkinTime="1555333493008" checkinUser="pensoft" docAuthor="Yang, Qin, Fan, Xin-Lei, Guarnaccia, Vladimiro &amp; Tian, Cheng-Ming" docDate="2018" docId="5B72B3409D375ABAC867FDABABB35A48" docLanguage="en" docName="MycoKeys 39: 97-149" docOrigin="MycoKeys 39" docSource="http://dx.doi.org/10.3897/mycokeys.39.26914" docTitle="Diaporthe eres Nitschke 1870" docType="treatment" docVersion="4" lastPageNumber="97" masterDocId="FFD50050FFFDA55CFF8A4E3BFFF3FFBB" masterDocTitle="High diversity of Diaporthe species associated with dieback diseases in China, with twelve new species described" masterLastPageNumber="149" masterPageNumber="97" pageNumber="97" updateTime="1668136060170" updateUser="ExternalLinkService">
<mods:mods xmlns:mods="http://www.loc.gov/mods/v3">
<mods:titleInfo>
<mods:title>High diversity of Diaporthe species associated with dieback diseases in China, with twelve new species described</mods:title>
</mods:titleInfo>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Yang, Qin</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Fan, Xin-Lei</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Guarnaccia, Vladimiro</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Tian, Cheng-Ming</mods:namePart>
</mods:name>
<mods:typeOfResource>text</mods:typeOfResource>
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<mods:titleInfo>
<mods:title>MycoKeys</mods:title>
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<mods:part>
<mods:date>2018</mods:date>
<mods:detail type="volume">
<mods:number>39</mods:number>
</mods:detail>
<mods:extent unit="page">
<mods:start>97</mods:start>
<mods:end>149</mods:end>
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<mods:url>http://dx.doi.org/10.3897/mycokeys.39.26914</mods:url>
</mods:location>
<mods:classification>journal article</mods:classification>
<mods:identifier type="DOI">http://dx.doi.org/10.3897/mycokeys.39.26914</mods:identifier>
<mods:identifier type="Pensoft-Pub">1314-4049-39-97</mods:identifier>
</mods:mods>
<treatment ID-GBIF-Taxon="156202285" LSID="urn:lsid:plazi:treatment:5B72B3409D375ABAC867FDABABB35A48" httpUri="http://treatment.plazi.org/id/5B72B3409D375ABAC867FDABABB35A48" lastPageNumber="97" pageId="0" pageNumber="97">
<subSubSection pageId="0" pageNumber="97" type="nomenclature">
<paragraph pageId="0" pageNumber="97">
<taxonomicName authority="Nitschke, 1870" authorityName="Nitschke" authorityYear="1870" class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe eres" order="Diaporthales" pageId="0" pageNumber="97" phylum="Ascomycota" rank="species" species="eres">Diaporthe eres Nitschke, 1870</taxonomicName>
Figure 12
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="97" type="reference_group">
<paragraph pageId="0" pageNumber="97">
=
<taxonomicName class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe biguttusis" order="Diaporthales" pageId="0" pageNumber="97" phylum="Ascomycota" rank="species" species="biguttusis">Diaporthe biguttusis</taxonomicName>
Y.H. Gao &amp; L. Cai, 2015.
</paragraph>
<paragraph pageId="0" pageNumber="97">
=
<taxonomicName class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe camptothecicola" order="Diaporthales" pageId="0" pageNumber="97" phylum="Ascomycota" rank="species" species="camptothecicola">Diaporthe camptothecicola</taxonomicName>
C.M. Tian &amp; Qin Yang, 2017.
</paragraph>
<paragraph pageId="0" pageNumber="97">
=
<taxonomicName class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe ellipicola" order="Diaporthales" pageId="0" pageNumber="97" phylum="Ascomycota" rank="species" species="ellipicola">Diaporthe ellipicola</taxonomicName>
Y.H. Gao &amp; L. Cai, 2015.
</paragraph>
<paragraph pageId="0" pageNumber="97">
=
<taxonomicName class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe longicicola" order="Diaporthales" pageId="0" pageNumber="97" phylum="Ascomycota" rank="species" species="longicicola">Diaporthe longicicola</taxonomicName>
Y.H. Gao &amp; L. Cai, 2015
</paragraph>
<paragraph pageId="0" pageNumber="97">
=
<taxonomicName class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe mahothocarpus" order="Diaporthales" pageId="0" pageNumber="97" phylum="Ascomycota" rank="species" species="mahothocarpus">Diaporthe mahothocarpus</taxonomicName>
(Y.H. Gao, W. Sun &amp; L. Cai) Y.H. Gao &amp; L. Cai, 2015.
</paragraph>
<paragraph pageId="0" pageNumber="97">
=
<taxonomicName class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe momicola" order="Diaporthales" pageId="0" pageNumber="97" phylum="Ascomycota" rank="species" species="momicola">Diaporthe momicola</taxonomicName>
Dissan., J.Y. Yan, Xing H. Li &amp; K.D. Hyde, 2017.
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="97" type="description">
<paragraph pageId="0" pageNumber="97">Description.</paragraph>
<paragraph pageId="0" pageNumber="97">
Conidiomata pycnidial, immersed in bark, erumpent through the bark surface, serried, with a single locule. Ectostromatic disc obviously, brown to black, with one ostiole per disc, 245-572
<normalizedToken originalValue="μm">μm</normalizedToken>
diam. Ostiole medium black, up to the level of disc. Locule circular, undivided, 335-450
<normalizedToken originalValue="μm">μm</normalizedToken>
diam. Conidiophores 10.5-19
<normalizedToken originalValue="×">x</normalizedToken>
1-1.5
<normalizedToken originalValue="μm">μm</normalizedToken>
, cylindrical, hyaline, unbranched, straight or slightly sinuous. Conidiogenous cells phialidic, cylindrical, terminal. Alpha conidia hyaline, aseptate, ellipsoidal to lanceolate, one guttulate at each end, 6-7.5
<normalizedToken originalValue="×">x</normalizedToken>
1.5-2.5
<normalizedToken originalValue="μm">μm</normalizedToken>
(av. = 6.5
<normalizedToken originalValue="×">x</normalizedToken>
2
<normalizedToken originalValue="μm">μm</normalizedToken>
, n = 30). Beta conidia not observed.
</paragraph>
<caption pageId="0" pageNumber="97">
<paragraph pageId="0" pageNumber="97">
Figure 12.
<taxonomicName class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe eres" order="Diaporthales" pageId="0" pageNumber="97" phylum="Ascomycota" rank="species" species="eres">Diaporthe eres</taxonomicName>
(CFCC 52575)
<normalizedToken originalValue="AB">A-B</normalizedToken>
Habit of conidiomata on branches C Transverse section of conidioma D Longitudinal section of conidioma E Alpha conidia F Conidiophores G Culture on PDA and conidiomata. Scale bars: 500
<normalizedToken originalValue="μm">μm</normalizedToken>
(B), 200
<normalizedToken originalValue="μm">μm</normalizedToken>
(
<normalizedToken originalValue="CD">C-D</normalizedToken>
), 10
<normalizedToken originalValue="μm">μm</normalizedToken>
(
<normalizedToken originalValue="EF">E-F</normalizedToken>
).
</paragraph>
</caption>
</subSubSection>
<subSubSection pageId="0" pageNumber="97" type="description">
<paragraph pageId="0" pageNumber="97">Culture characters.</paragraph>
<paragraph pageId="0" pageNumber="97">Cultures on PDA incubated at 25 °C in darkness. Colony with white felty aerial mycelium, becoming white felted aerial mycelium in the centre and grey-brown mycelium at the marginal area, conidiomata irregularly distributed over agar surface.</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="97" type="materials_examined">
<paragraph pageId="0" pageNumber="97">Specimens examined.</paragraph>
<paragraph pageId="0" pageNumber="97">
CHINA. Beijing: Pinggu district, on symptomatic branches of
<taxonomicName class="Magnoliopsida" family="Fagaceae" genus="Castanea" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Castanea mollissima" order="Fagales" pageId="0" pageNumber="97" phylum="Tracheophyta" rank="species" species="mollissima">Castanea mollissima</taxonomicName>
, 1 Nov. 2016, N. Jiang, living culture CFCC 52576 (BJFC-S1489); ibid. living culture CFCC 52577 (BJFC-S1490). Heilongjiang Province: Liangshui Nature Reserve, on symptomatic twigs of
<taxonomicName class="Magnoliopsida" family="Araliaceae" genus="Acanthopanax" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Acanthopanax senticosus" order="Apiales" pageId="0" pageNumber="97" phylum="Tracheophyta" rank="species" species="senticosus">Acanthopanax senticosus</taxonomicName>
, 29 July 2016, Q. Yang, living culture CFCC 52580 (BJFC-S1493). Heilongjiang Province: Harbin city, Botanical garden, on symptomatic twigs of
<taxonomicName class="Magnoliopsida" family="Rosaceae" genus="Sorbus" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Sorbus" order="Rosales" pageId="0" pageNumber="97" phylum="Tracheophyta" rank="genus">Sorbus</taxonomicName>
sp., 2 Aug. 2016, Q. Yang, living culture CFCC 52575 (BJFC-S1488). Shaanxi Province: Zhashui County, on symptomatic branches of
<taxonomicName class="Magnoliopsida" family="Juglandaceae" genus="Juglans" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Juglans regia" order="Fagales" pageId="0" pageNumber="97" phylum="Tracheophyta" rank="species" species="regia">Juglans regia</taxonomicName>
, 29 July 2016, Q. Yang, living culture CFCC 52579 (BJFC-S1492). Zhejiang Province: Yangzhou city, on symptomatic twigs of
<taxonomicName class="Insecta" family="Tachinidae" genus="Melia" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Melia azedarace" order="Diptera" pageId="0" pageNumber="97" phylum="Arthropoda" rank="species" species="azedarace">Melia azedarace</taxonomicName>
, 8 July 2017, N. Jiang, living culture CFCC 52578 (BJFC-S1491). Zhejiang Province: Tianmu Mountain, on symptomatic twigs of
<taxonomicName class="Magnoliopsida" family="Ericaceae" genus="Rhododendron" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Rhododendron simsii" order="Ericales" pageId="0" pageNumber="97" phylum="Tracheophyta" rank="species" species="simsii">Rhododendron simsii</taxonomicName>
, 20 Apr. 2017, Q. Yang, living culture CFCC 52581 (BJFC-S1494).
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="97" type="notes">
<paragraph pageId="0" pageNumber="97">Notes.</paragraph>
<paragraph pageId="0" pageNumber="97">
<taxonomicName class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe eres" order="Diaporthales" pageId="0" pageNumber="97" phylum="Ascomycota" rank="species" species="eres">Diaporthe eres</taxonomicName>
, the type species of the genus, was described by
<bibRefCitation pageId="0" pageNumber="97">Nitschke (1870)</bibRefCitation>
on
<taxonomicName class="Insecta" family="Tingidae" genus="Ulmus" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Ulmus" order="Hemiptera" pageId="0" pageNumber="97" phylum="Arthropoda" rank="genus">Ulmus</taxonomicName>
sp. collected in Germany, which has a widespread distribution and a broad host range as a pathogen, endophyte or saprobe causing leaf spots, stem cankers and diseases of woody plants (
<bibRefCitation author="Udayanga, D" journalOrPublisher="Fungal Diversity" pageId="0" pageNumber="97" pagination="203 - 229" title="Insights into the genus Diaporthe: phylogenetic species delimitation in the D. eres species complex." url="https://doi.org/10.1007/s13225-014-0297-2" volume="67" year="2014 b">Udayanga et al. 2014b</bibRefCitation>
).
<bibRefCitation author="Fan, XL" journalOrPublisher="Mycological Progress" pageId="0" pageNumber="97" url="https://doi.org/10.1007/s11557-018-1395-4" year="2018">Fan et al. (2018)</bibRefCitation>
indicated that
<taxonomicName lsidName="D. biguttusis" pageId="0" pageNumber="97" rank="species" species="biguttusis">D. biguttusis</taxonomicName>
,
<taxonomicName lsidName="D. ellipicola" pageId="0" pageNumber="97" rank="species" species="ellipicola">D. ellipicola</taxonomicName>
,
<taxonomicName lsidName="D. longicicola" pageId="0" pageNumber="97" rank="species" species="longicicola">D. longicicola</taxonomicName>
and
<taxonomicName lsidName="D. mahothocarpus" pageId="0" pageNumber="97" rank="species" species="mahothocarpus">D. mahothocarpus</taxonomicName>
should be treated as synonyms of
<taxonomicName lsidName="D. eres" pageId="0" pageNumber="97" rank="species" species="eres">D. eres</taxonomicName>
using cal, tef1 and tub2 gene regions. In this study, we extended the work presented in
<bibRefCitation author="Fan, XL" journalOrPublisher="Mycological Progress" pageId="0" pageNumber="97" url="https://doi.org/10.1007/s11557-018-1395-4" year="2018">Fan et al. (2018)</bibRefCitation>
and found seven additional strains belonging to
<taxonomicName lsidName="D. eres" pageId="0" pageNumber="97" rank="species" species="eres">D. eres</taxonomicName>
. Additionally, the phylogenetic tree demonstrated that
<taxonomicName lsidName="D. camptothecicola" pageId="0" pageNumber="97" rank="species" species="camptothecicola">D. camptothecicola</taxonomicName>
and
<taxonomicName lsidName="D. momicola" pageId="0" pageNumber="97" rank="species" species="momicola">D. momicola</taxonomicName>
should also be treated as synonyms of
<taxonomicName lsidName="D. eres" pageId="0" pageNumber="97" rank="species" species="eres">D. eres</taxonomicName>
(Fig. 2).
<taxonomicName class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe camptothecicola" order="Diaporthales" pageId="0" pageNumber="97" phylum="Ascomycota" rank="species" species="camptothecicola">Diaporthe camptothecicola</taxonomicName>
from
<taxonomicName class="Magnoliopsida" family="Nyssaceae" genus="Camptotheca" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Camptotheca acuminate" order="Cornales" pageId="0" pageNumber="97" phylum="Tracheophyta" rank="species" species="acuminate">Camptotheca acuminate</taxonomicName>
and
<taxonomicName lsidName="D. momicola" pageId="0" pageNumber="97" rank="species" species="momicola">D. momicola</taxonomicName>
from
<taxonomicName class="Magnoliopsida" family="Rosaceae" genus="Prunus" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Prunus persica" order="Rosales" pageId="0" pageNumber="97" phylum="Tracheophyta" rank="species" species="persica">Prunus persica</taxonomicName>
are described and illustrated based on the combined ITS, cal, his3, tef1 and tub2 regions (
<bibRefCitation author="Dissanayake, AJ" journalOrPublisher="Mycosphere" pageId="0" pageNumber="97" pagination="533 - 549" title="Diaporthe species associated with peach tree dieback in Hubei, China." url="https://doi.org/10.5943/mycosphere/8/5/3" volume="8" year="2017 a">Dissanayake et al. 2017a</bibRefCitation>
,
<bibRefCitation author="Yang, Q" journalOrPublisher="Mycotaxon" pageId="0" pageNumber="97" pagination="591 - 601" title="Diaporthecamptothecicola sp. nov. on Camptothecaacuminata in China." url="https://doi.org/10.5248/132.591" volume="132" year="2017 c">Yang et al. 2017c</bibRefCitation>
). Both of the two species are embedded in the
<taxonomicName lsidName="D. eres" pageId="0" pageNumber="97" rank="species" species="eres">D. eres</taxonomicName>
complex. However, ITS analysis resulted in an unresolved phylogenetic tree without definitive bootstrap at the internodes, highly discordant to the trees resulting from the other four genes (
<bibRefCitation author="Udayanga, D" journalOrPublisher="Fungal Diversity" pageId="0" pageNumber="97" pagination="203 - 229" title="Insights into the genus Diaporthe: phylogenetic species delimitation in the D. eres species complex." url="https://doi.org/10.1007/s13225-014-0297-2" volume="67" year="2014 b">Udayanga et al. 2014b</bibRefCitation>
). Therefore, the ITS region was not used in the combined analysis in the current study. To further investigate this complex, a second set of four (cal, his3, tef1 and tub2), three (cal, tef1 and tub2), two (tef1 and tub2) and one (tef1) data matrices were performed following
<bibRefCitation author="Santos, L" journalOrPublisher="Persoonia: Molecular Phylogeny and Evolution of Fungi" pageId="0" pageNumber="97" url="https://doi.org/10.7717/peerj.3120" year="2017">Santos et al. (2017)</bibRefCitation>
and
<bibRefCitation author="Fan, XL" journalOrPublisher="Mycological Progress" pageId="0" pageNumber="97" url="https://doi.org/10.1007/s11557-018-1395-4" year="2018">Fan et al. (2018)</bibRefCitation>
. The results showed that the three genes analyses (cal, tef1 and tub2) appeared to be a better species recognition (Fig. 2). When it comes to this species complex, sequences supported by
<bibRefCitation author="Udayanga, D" journalOrPublisher="Fungal Diversity" pageId="0" pageNumber="97" pagination="203 - 229" title="Insights into the genus Diaporthe: phylogenetic species delimitation in the D. eres species complex." url="https://doi.org/10.1007/s13225-014-0297-2" volume="67" year="2014 b">Udayanga et al. (2014b)</bibRefCitation>
are necessary to perform a more robust phylogenetic tree, clarifying the real species boundaries in this group in the future work.
</paragraph>
</subSubSection>
</treatment>
</document>