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<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.40.28636" ID-GBIF-Dataset="44a6c244-d759-488e-971e-74fc6a8db89a" ID-PMC="PMC6160797" ID-Pensoft-Pub="1314-4049-40-29" ID-PubMed="30271263" ModsDocAuthor="" ModsDocDate="2018" ModsDocID="1314-4049-40-29" ModsDocOrigin="MycoKeys 40" ModsDocTitle="Liberomycespistaciae sp. nov., the causal agent of pistachio cankers and decline in Italy" checkinTime="1555333482224" checkinUser="pensoft" docAuthor="Vitale, Salvatore, Aiello, Dalia, Guarnaccia, Vladimiro, Luongo, Laura, Galli, Massimo, Crous, Pedro W., Polizzi, Giancarlo, Belisario, Alessandra &amp; Voglmayr, Hermann" docDate="2018" docId="76695275A9E5833AFEA0AF602204AAA1" docLanguage="en" docName="MycoKeys 40: 29-51" docOrigin="MycoKeys 40" docSource="http://dx.doi.org/10.3897/mycokeys.40.28636" docTitle="Liberomyces pistaciae Voglmayr, S. Vitale, D. Aiello, Guarnaccia, Luongo &amp; Belisario, sp. nov." docType="treatment" docVersion="4" lastPageNumber="34" masterDocId="FF81FFDDFFE0C924FFF0FFFD5565FFEE" masterDocTitle="Liberomycespistaciae sp. nov., the causal agent of pistachio cankers and decline in Italy" masterLastPageNumber="51" masterPageNumber="29" pageNumber="32" updateTime="1668136068663" updateUser="ExternalLinkService">
<mods:mods xmlns:mods="http://www.loc.gov/mods/v3">
<mods:titleInfo>
<mods:title>Liberomycespistaciae sp. nov., the causal agent of pistachio cankers and decline in Italy</mods:title>
</mods:titleInfo>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Vitale, Salvatore</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Aiello, Dalia</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Guarnaccia, Vladimiro</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Luongo, Laura</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Galli, Massimo</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Crous, Pedro W.</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Polizzi, Giancarlo</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Belisario, Alessandra</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Voglmayr, Hermann</mods:namePart>
</mods:name>
<mods:typeOfResource>text</mods:typeOfResource>
<mods:relatedItem type="host">
<mods:titleInfo>
<mods:title>MycoKeys</mods:title>
</mods:titleInfo>
<mods:part>
<mods:date>2018</mods:date>
<mods:detail type="volume">
<mods:number>40</mods:number>
</mods:detail>
<mods:extent unit="page">
<mods:start>29</mods:start>
<mods:end>51</mods:end>
</mods:extent>
</mods:part>
</mods:relatedItem>
<mods:location>
<mods:url>http://dx.doi.org/10.3897/mycokeys.40.28636</mods:url>
</mods:location>
<mods:classification>journal article</mods:classification>
<mods:identifier type="DOI">http://dx.doi.org/10.3897/mycokeys.40.28636</mods:identifier>
<mods:identifier type="Pensoft-Pub">1314-4049-40-29</mods:identifier>
</mods:mods>
<treatment ID-GBIF-Taxon="156202255" LSID="urn:lsid:plazi:treatment:76695275A9E5833AFEA0AF602204AAA1" httpUri="http://treatment.plazi.org/id/76695275A9E5833AFEA0AF602204AAA1" lastPageId="5" lastPageNumber="34" pageId="3" pageNumber="32">
<subSubSection pageId="3" pageNumber="32" type="nomenclature">
<paragraph pageId="3" pageNumber="32">
<taxonomicName LSID="827682" authority="Voglmayr, S. Vitale, D. Aiello, Guarnaccia, Luongo &amp; Belisario" family="Delonicicolaceae" genus="Liberomyces" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Liberomyces pistaciae" order="Xylariales" pageId="3" pageNumber="32" phylum="Ascomycota" rank="species" species="pistaciae">Liberomyces pistaciae Voglmayr, S. Vitale, D. Aiello, Guarnaccia, Luongo &amp; Belisario</taxonomicName>
<taxonomicNameLabel pageId="3" pageNumber="32">sp. nov.</taxonomicNameLabel>
Fig. 6
</paragraph>
</subSubSection>
<subSubSection pageId="3" pageNumber="32" type="diagnosis">
<paragraph pageId="3" pageNumber="32">Diagnosis.</paragraph>
<paragraph pageId="3" pageNumber="32">
Species with distinctly smaller conidia (3.2-5.0
<normalizedToken originalValue="×">x</normalizedToken>
1.0-2.0
<normalizedToken originalValue="μm">μm</normalizedToken>
) than in
<taxonomicName genus="Liberomyces" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Liberomyces saliciphilus" pageId="3" pageNumber="32" phylum="Ascomycota" rank="species" species="saliciphilus">Liberomyces saliciphilus</taxonomicName>
<normalizedToken originalValue="Pažoutová">Pazoutova</normalizedToken>
, M.
<normalizedToken originalValue="Kolařík">Kolarik</normalizedToken>
&amp;
<normalizedToken originalValue="Kubátová">Kubatova</normalizedToken>
and
<taxonomicName lsidName="L. macrosporus" pageId="3" pageNumber="32" rank="species" species="macrosporus">L. macrosporus</taxonomicName>
<normalizedToken originalValue="Pažoutová">Pazoutova</normalizedToken>
, M.
<normalizedToken originalValue="Kolařík">Kolarik</normalizedToken>
&amp;
<normalizedToken originalValue="Kubátová">Kubatova</normalizedToken>
.
</paragraph>
</subSubSection>
<subSubSection pageId="3" pageNumber="32" type="materials_examined">
<paragraph pageId="3" pageNumber="32">Type.</paragraph>
<paragraph pageId="3" pageNumber="32">
ITALY. Sicily: Bronte (Catania province), on cankered twig of
<taxonomicName class="Magnoliopsida" family="Anacardiaceae" genus="Pistacia" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Pistacia vera" order="Sapindales" pageId="3" pageNumber="32" phylum="Tracheophyta" rank="species" species="vera">Pistacia vera</taxonomicName>
, June 2010, A. Belisario (holotype: WU 39967; ex-type culture CBS 128196 = ISPaVe1958).
</paragraph>
</subSubSection>
<subSubSection pageId="3" pageNumber="32" type="etymology">
<paragraph pageId="3" pageNumber="32">Etymology.</paragraph>
<paragraph pageId="3" pageNumber="32">
Named after its host genus,
<taxonomicName class="Magnoliopsida" family="Anacardiaceae" genus="Pistacia" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Pistacia" order="Sapindales" pageId="3" pageNumber="32" phylum="Tracheophyta" rank="genus">Pistacia</taxonomicName>
.
</paragraph>
</subSubSection>
<subSubSection lastPageId="5" lastPageNumber="34" pageId="4" pageNumber="33" type="description">
<paragraph pageId="4" pageNumber="33">
<pageBreakToken pageId="4" pageNumber="33" start="start">Description</pageBreakToken>
.
</paragraph>
<paragraph lastPageId="5" lastPageNumber="34" pageId="4" pageNumber="33">
<taxonomicName genus="Conidiomata" lsidName="Conidiomata" pageId="4" pageNumber="33" rank="genus">Conidiomata</taxonomicName>
pycnidial, superficial or immersed, single to densely aggregated, subglobose or cupular, uni- or irregularly plurilocular, first hyaline to pale brown, turning dark brown to blackish, without ostiole, irregularly rupturing at the
<pageBreakToken pageId="5" pageNumber="34" start="start">apex</pageBreakToken>
and exuding a pale whitish conidial drop at maturity, (100
<normalizedToken originalValue=")170260(">-)170-260(-</normalizedToken>
330)
<normalizedToken originalValue="µm">µm</normalizedToken>
diam. (n=40). Pycnidial wall thin, of pale brown cells, (2.0
<normalizedToken originalValue=")3.56.3(">-)3.5-6.3(-</normalizedToken>
10.0)
<normalizedToken originalValue="μm">μm</normalizedToken>
diam. (n=162) forming a textura angularis, outside darker, thicker-walled and more rounded, inside lined by a layer of angular hyaline cells giving rise to conidiophores. Conidiophores short, densely fasciculate, up to three times branched, hyaline, smooth, arising from the inner wall of the entire conidioma, 10-28
<normalizedToken originalValue="µm">µm</normalizedToken>
long. Conidiogenous cells holoblastic with sympodial proliferation, lageniform to cylindrical, (5.5
<normalizedToken originalValue=")6.58.5(">-)6.5-8.5(-</normalizedToken>
10.0)
<normalizedToken originalValue="×">x</normalizedToken>
1.7
<normalizedToken originalValue="2.5(">-2.5(-</normalizedToken>
2.7)
<normalizedToken originalValue="µm">µm</normalizedToken>
(n=52), in dense intercalary or terminal whorls of 2-9. Conidia straight to allantoid, hyaline, smooth, 1-celled, (3.2
<normalizedToken originalValue=")3.84.5(">-)3.8-4.5(-</normalizedToken>
5.0)
<normalizedToken originalValue="×">x</normalizedToken>
(1.0
<normalizedToken originalValue=")1.21.5(">-)1.2-1.5(-</normalizedToken>
2.0)
<normalizedToken originalValue="μm">μm</normalizedToken>
, l/w = (2.0
<normalizedToken originalValue=")2.73.5(">-)2.7-3.5(-</normalizedToken>
4.7)
<normalizedToken originalValue="µm">µm</normalizedToken>
(n=182).
</paragraph>
</subSubSection>
<subSubSection pageId="5" pageNumber="34" type="description">
<paragraph pageId="5" pageNumber="34">Culture characteristics.</paragraph>
<paragraph pageId="5" pageNumber="34">Colonies slow-growing (about 4 cm in diam. in 1 month on MEA, 4 cm in 2 weeks on CMD at 22 °C), initially white, turning pale to dark brown with age, with a whitish slightly lobed margin (Fig. 6a and b), surface mycelium sparse. Red to brown pigments diffusing in growth medium. Densely aggregated pycnidia formed after 7 d on the inoculum plug, successively also on the colony surface.</paragraph>
</subSubSection>
<subSubSection pageId="5" pageNumber="34" type="notes">
<paragraph pageId="5" pageNumber="34">Notes.</paragraph>
<paragraph pageId="5" pageNumber="34">
Morphologically,
<taxonomicName genus="Liberomyces" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Liberomyces pistaciae" pageId="5" pageNumber="34" phylum="Ascomycota" rank="species" species="pistaciae">Liberomyces pistaciae</taxonomicName>
is similar to the other two species of the genus,
<taxonomicName lsidName="L. macrosporus" pageId="5" pageNumber="34" rank="species" species="macrosporus">L. macrosporus</taxonomicName>
and
<taxonomicName lsidName="L. saliciphilus" pageId="5" pageNumber="34" rank="species" species="saliciphilus">L. saliciphilus</taxonomicName>
, but the latter have distinctly longer conidia (5-7.5
<normalizedToken originalValue="µm">µm</normalizedToken>
in
<taxonomicName lsidName="L. saliciphilus" pageId="5" pageNumber="34" rank="species" species="saliciphilus">L. saliciphilus</taxonomicName>
, 8-13
<normalizedToken originalValue="µm">µm</normalizedToken>
in
<taxonomicName lsidName="L. macrosporus" pageId="5" pageNumber="34" rank="species" species="macrosporus">L. macrosporus</taxonomicName>
).
</paragraph>
<caption pageId="5" pageNumber="34">
<paragraph pageId="5" pageNumber="34">
Figure 6.
<taxonomicName genus="Liberomyces" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Liberomyces pistaciae" pageId="5" pageNumber="34" phylum="Ascomycota" rank="species" species="pistaciae">Liberomyces pistaciae</taxonomicName>
.
<normalizedToken originalValue="ad">a-d</normalizedToken>
Cultures (aMEA, 6 weeks, 22 °C bCMD, 6 weeks, 22 °C cPDA, 3 weeks, 25 °C dPDA, 2 weeks, 25 °C) e Pycnidia produced on artificially inoculated sterilised pistachio twigs
<normalizedToken originalValue="fh">f-h</normalizedToken>
Pycnidia in face view on MEAi Pycnidial wall in face view
<normalizedToken originalValue="jn">j-n</normalizedToken>
Conidiophores and conidiogenous cells
<normalizedToken originalValue="oq">o-q</normalizedToken>
Conidiogenous cells (o young p, q showing sympodial conidiation) r Conidia. All in water. Sources:
<normalizedToken originalValue="ac">a-c</normalizedToken>
,
<normalizedToken originalValue="fr">f-r</normalizedToken>
ex-holotype strain ISPaVe1958 = CBS 128196 d, e PV1= CPC 31292. Scale bars: 500
<normalizedToken originalValue="µm">µm</normalizedToken>
(e, f); 200
<normalizedToken originalValue="µm">µm</normalizedToken>
(g, h); 10
<normalizedToken originalValue="µm">µm</normalizedToken>
(
<normalizedToken originalValue="il">i-l</normalizedToken>
); 5
<normalizedToken originalValue="µm">µm</normalizedToken>
(
<normalizedToken originalValue="mr">m-r</normalizedToken>
).
</paragraph>
</caption>
</subSubSection>
</treatment>
</document>