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<mods:title id="D67CDEDD7E2513A0096565746235D292">Description of two new species, Aphanolaimus strilliae n. sp. (Nematoda: Aphanolaimidae) and Makatinus africanus n. sp. (Nematoda: Aporcelaimidae), and SEM observations on three known species from freshwater sources in the Telperion Nature Reserve (Mpumalanga, South Africa)</mods:title>
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<heading id="D0AB81CFA8139D5E4888531BFC6BFAD8" bold="true" box="[151,917,1353,1380]" fontSize="11" level="1" pageId="20" pageNumber="221" reason="1">
<taxonomicName id="4C5C4D20A8139D5E4888531BFC6BFAD8" ID-CoL="8GDS6" authority="(Joubert &amp; Heyns, 1979) Tsalolikhin, 1981" authorityName="Tsalolikhin" authorityYear="1981" baseAuthorityName="Joubert &amp; Heyns" baseAuthorityYear="1979" box="[151,917,1353,1380]" class="Enoplea" family="Tobrilidae" genus="Neotobrilus" higherTaxonomySource="GBIF" kingdom="Animalia" order="Triplonchida" pageId="20" pageNumber="221" phylum="Nematoda" rank="species" species="ampiei">
<emphasis id="B928EAB1A8139D5E4888531BFC6BFAD8" bold="true" box="[151,917,1353,1380]" pageId="20" pageNumber="221">
<emphasis id="B928EAB1A8139D5E4888531BFE83FAD8" bold="true" box="[151,381,1353,1380]" italics="true" pageId="20" pageNumber="221">Neotobrilus ampiei</emphasis>
(
<bibRefCitation id="EFCD4B52A8139D5E4992531BFD4FFADF" author="Joubert, A. P. &amp; Heyns, J." box="[397,689,1353,1380]" pageId="20" pageNumber="221" pagination="17 - 26" refId="ref15185" refString="Joubert, A. P. &amp; Heyns, J. (1979) Freshwater nematodes from South Africa. 3. Tobrilus Andrassy, 1959. Journal of the Limnological Society of Southern Africa, 5 (1), 17 - 26. https: // doi. org / 10.1080 / 03779688.1979.9633176" type="journal article" year="1979">Joubert &amp; Heyns, 1979</bibRefCitation>
)
<bibRefCitation id="EFCD4B52A8139D5E4ADF5318FC6BFAD8" author="Tsalolikhin, S. Ya." box="[704,917,1354,1380]" pageId="20" pageNumber="221" pagination="1302 - 1313" refId="ref16176" refString="Tsalolikhin, S. Ya. (1981) Revision of the genus Tobrilus (Nematoda-Tobrilidae). Zoologicheskii Zhurnal, 60, 1302 - 1313. [in Russian]" type="journal article" year="1981">Tsalolikhin, 1981</bibRefCitation>
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<paragraph id="8BE336A3A8139D5E4888533CFEC6FA34" blockId="20.[151,917,1353,1416]" box="[151,312,1390,1416]" pageId="20" pageNumber="221">
<figureCitation id="13672A26A8139D5E4888533CFEC6FA34" box="[151,312,1390,1416]" captionStart-0="FIGURE 14" captionStart-1="FIGURE 15" captionStart-2="FIGURE 16" captionStart-3="FIGURE 17" captionStart-4="FIGURE 18" captionStart-5="FIGURE 19" captionStart-6="FIGURE 20" captionStart-7="FIGURE 21" captionStartId-0="22.[151,250,1482,1507]" captionStartId-1="26.[151,250,1045,1070]" captionStartId-2="26.[151,250,1871,1896]" captionStartId-3="27.[151,250,1497,1522]" captionStartId-4="28.[151,250,1942,1967]" captionStartId-5="29.[151,250,1937,1962]" captionStartId-6="30.[151,250,1058,1083]" captionStartId-7="30.[151,250,1966,1991]" captionTargetBox-0="[164,1426,238,1395]" captionTargetBox-1="[218,1373,190,1020]" captionTargetBox-2="[170,1416,1164,1852]" captionTargetBox-3="[151,1436,181,1477]" captionTargetBox-4="[270,1318,489,1922]" captionTargetBox-5="[300,1287,166,1917]" captionTargetBox-6="[256,1331,181,1038]" captionTargetBox-7="[240,1348,1176,1945]" captionTargetId-0="figure@22.[151,1436,234,1402]" captionTargetId-1="figure@26.[203,1384,181,1025]" captionTargetId-2="figure@26.[170,1416,1164,1852]" captionTargetId-3="figure@27.[151,1436,181,1477]" captionTargetId-4="figure@28.[270,1318,489,1922]" captionTargetId-5="figure@29.[300,1287,166,1917]" captionTargetId-6="figure@30.[256,1331,181,1038]" captionTargetId-7="figure@30.[240,1348,1176,1945]" captionTargetPageId-0="22" captionTargetPageId-1="26" captionTargetPageId-2="26" captionTargetPageId-3="27" captionTargetPageId-4="28" captionTargetPageId-5="29" captionTargetPageId-6="30" captionTargetPageId-7="30" captionText-0="FIGURE 14. Female drawings Neotobrilus ampiei (Joubert &amp; Heyns, 1979) Tsalolikhin, 1981 A) Anterior region showing oesophagus; B) Tail; C) Vulval region showing musculature of vagina; D) Anterior region showing structure of stoma and teeth pockets; E) Vulval region showing part of anterior genital tract with two eggs and full posterior tract with one egg." captionText-1="FIGURE 15. Male drawings Neotobrilus ampiei (Joubert &amp; Heyns, 1979) Tsalolikhin, 1981 A) Close up of supplements 36 showing position of micropapillae; B) Supplementary row S1S6 (S1 starting anteriorly); Ductus ejaculatoris; spicule and gu- bernaculum; C) Anterior region showing stoma and arrangement of teeth pockets." captionText-2="FIGURE 16. Scanning electron micrograph of Neotobrilus ampiei (Joubert &amp; Heyns, 1979) Tsalolikhin, 1981; A) Lateral view of anterior end showing amphid (a); long cephalic setae (lcs), short cephalic setae (scc) and labial papillae (lp); B) Anterior end showing long cephalic setae (lcs), short cephalic setae (scc) and labial papillae (lp) and liplets (l); C) Close up of long cephalic setae (lcs) and labial papillae (lp), arrows indicating the opening of the sensory organs; D) Arrow indicating lateral cord; E) Ventral side of body showing vulva." captionText-3="FIGURE 17. Scanning electron micrograph of Neotobrilus ampiei (Joubert &amp; Heyns, 1979) Tsalolikhin, 1981 A) Female tale showing spinneret and last somatic (caudal setae); BC) Close up of tip of spicule (s) showing medial outgrowth; D) Male posterior part showing supplements S1S6 and a row of ventrosublateral setae." captionText-4="FIGURE 18. Scanning electron micrograph of Neotobrilus ampiei (Joubert &amp; Heyns, 1979) Tsalolikhin, 1981 ventral side of male showing distribution and direction of ventrosublateral setae (vsls); supplements S4S6, and micropappilae (small ar- rows)." captionText-5="FIGURE 19. Scanning electron micrograph of Neotobrilus ampiei (Joubert &amp; Heyns, 1979) Tsalolikhin, 1981 A + C) Close up of supplements S2 and S3 (S1 identical to S2 and S3); B) Close up of ventrosublateral setae, arrow showing junction where two parts meet; DF) Close up of supplement S4S6." captionText-6="FIGURE 20. Light microscopic pictures of Neotobrilus ampiei (Joubert &amp; Heyns, 1979) Tsalolikhin, 1981 female A) Vaginal region with three uterine eggs; B) Anterior end to end of posterior genital tract arrow indicating vulva; C) Labial region showing buccal cavity and two tooth pockets (T1 and T2)." captionText-7="FIGURE 21. Light microscopic pictures of Neotobrilus ampiei (Joubert &amp; Heyns, 1979) Tsalolikhin, 1981 Male A); Posterior region showing supplements S1S4 and Ductus ejaculatoris (DE); B) Arrow indicating flaggelloid sperm cells in male." httpUri-0="https://zenodo.org/record/3363109/files/figure.png" httpUri-1="https://zenodo.org/record/3363111/files/figure.png" httpUri-2="https://zenodo.org/record/3363113/files/figure.png" httpUri-3="https://zenodo.org/record/3363115/files/figure.png" httpUri-4="https://zenodo.org/record/3363117/files/figure.png" httpUri-5="https://zenodo.org/record/3363119/files/figure.png" httpUri-6="https://zenodo.org/record/3363121/files/figure.png" httpUri-7="https://zenodo.org/record/3363123/files/figure.png" pageId="20" pageNumber="221">Figures 1421</figureCitation>
</paragraph>
<paragraph id="8BE336A3A8139D5E488853E4FE62FA6C" blockId="20.[151,1437,1461,2028]" box="[151,412,1461,1488]" pageId="20" pageNumber="221">
Measurements:
<tableCitation id="C6DE0318A8139D5E495653E4FE62FA6C" box="[329,412,1461,1488]" captionStart="TABLE 5" captionStartId="23.[151,239,472,498]" captionTargetPageId="23" captionText="TABLE 5. Morphometrics of three populations of Eutobrilus annetteae (Joubert &amp; Heyns, 1979) Tsalolikhin, 1983 and one population of Neotobrilus ampiei (Joubert &amp; Heyns, 1979) Tsalolikhin, 1981 found in freshwater substrates in the Telperion Nature Reserve. All measurements in the form: mean ± standard deviation (range)." httpUri="http://table.plazi.org/id/DF23662BA8109D5D4888578AF8FBFDAA" pageId="20" pageNumber="221" tableUuid="DF23662BA8109D5D4888578AF8FBFDAA">Table 5</tableCitation>
</paragraph>
<paragraph id="8BE336A3A8139D5F48D85388FB2AFF47" blockId="20.[151,1437,1461,2028]" lastBlockId="21.[151,1437,153,683]" lastPageId="21" lastPageNumber="222" pageId="20" pageNumber="221">
Description: Female (n = 13): Habitus curved ventrally to various degrees, body
<date id="FFE21063A8139D5E4C465388FB29FA48" box="[1113,1239,1498,1524]" pageId="20" pageNumber="221" value="1868" valueMax="2439">18682439</date>
μm long. Cuticle 14 μm thick at mid-body, annulated. Somatic setae numerous, spread unevenly over entire body. First somatic se- tum located 2441 μm from anterior end. Lip region 2436 μm wide, cephalic setae 1316 μm long.
<emphasis id="B928EAB1A8139D5E4D165071FAA1F980" box="[1289,1375,1570,1596]" italics="true" pageId="20" pageNumber="221">En face</emphasis>
view of head indicates one circlet consisting of six labial papillae, six long cephalic setae and four short cephalic setae. Cephalic setae originate from the cuticle, while cephalic papillae originate subcuticular. All setae and papillae appear to be hollow tubes. Amphids stirrup shaped with slit-like apertures, located 1719 μm from anterior end. Stoma 3544 μm long, half the width of lip region, consisting of two parts: A distinct barrel-shaped buccal cavity separated by two separate pockets. Each pocket contains a tooth and are separated by a narrowing of the cavity. First tooth is 1824 μm from anterior end with second tooth 1316 μm posterior to first. Oesophagus cylindrical and muscular, 345437 μm long. Nerve ring not observed. Cardia 2236 μm long with three prominent cells situated at junction of cardia and oesophagus. Excretory pore not observed. Reproductive system didelpic-amphidelphic, anterior tract (195392 μm long) almost always slightly shorter than posterior tract (228430 μm). Vulva a transversal slit situ- ated at 3746 % of body. Vaginal musculature strong, vagina less than half corresponding body width. Advulval setae twice as long as somatic setae (mean length of 16.4 μm), located dorsally at one body width anterior and posterior to vulva. Uterine eggs observed in most female specimens, measuring 5168 μm
<date id="FFE21063A8139D5E4C8C5180FB37F850" box="[1171,1225,2001,2028]" pageId="20" pageNumber="221" value="1935-10">X 35</date>
56 μm with shell. Eggshell uniquely sculptured with several prominent tubercules.
<emphasis id="B928EAB1A8129D5F4B6D56C8FB86FF0F" box="[882,1144,153,179]" italics="true" pageId="21" pageNumber="222">Pars dilatata oviductus</emphasis>
rounded, containing flaggelloid sperm cells. Ovaries reflexed, oocytes arranged in single row except at growth point. Tail 255354 μm long ending in rounded tip with a 35 μm spinneret and a subterminal setum. Rectum 3147 μm long.
</paragraph>
<paragraph id="8BE336A3A8129D5F48D85757FE63FD17" blockId="21.[151,1437,153,683]" pageId="21" pageNumber="222">
Male (n = 5): Habitus curved ventrally, body 19902320 μm long. Morphology of stoma and oesophagus similar to that of female. Spicule 6980 μm long, medial outgrowth from ventral limb towards dorsal limb. Gu- bernaculum 1926 μm long. Reproductive system diorchic, sperm cells flaggelloid.
<emphasis id="B928EAB1A8129D5F4C54571CFAD6FEDB" box="[1099,1320,333,359]" italics="true" pageId="21" pageNumber="222">Ductus ejaculatoris</emphasis>
muscular, its length corresponds with length of supplementary row, never exceeding it. Tail 181211 μm long. Two rows of subventral somatic setae along row of supplements, setae directed anteriorly and consisting of two parts. Supplementary row consisting of six pre-cloacal supplements, three larger and three closest to the cloaca, smaller. The supplements lose complexity nearing the cloaca. Supplement 1 to supplement 3 (starting anteriad) are complex in structure with several tubercules, whereas Supplement 4-Supplement 6 less covered in tubercules and Supplement 6 the most simplistic. Distance between Supplement 2 and Supplement 3 is the shortest. Micro papillae present between supplements Supplement 3 and Supplement 4 (1011 micropapillae), Supplement 4 and Supplement 5 (45 micropapillae) and Supplement 5 and Supplement 6 (56 micropapillae). According to SEM micropapillae appear as thin transversal slits.
</paragraph>
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<emphasis id="B928EAB1A8129D5F4888512BFEE1F82E" bold="true" box="[151,287,1913,1938]" pageId="21" pageNumber="222">FIGURE 13.</emphasis>
Light microscopic pictures of
<taxonomicName id="4C5C4D20A8129D5F4A4D5128FB27F82E" authority="(Joubert &amp; Heyns, 1979) Tsalolikhin, 1983" authorityName="Tsalolikhin" authorityYear="1983" baseAuthorityName="Joubert &amp; Heyns" baseAuthorityYear="1979" box="[594,1241,1913,1938]" class="Enoplea" family="Tobrilidae" genus="Eutobrilus" higherTaxonomySource="GBIF" kingdom="Animalia" order="Triplonchida" pageId="21" pageNumber="222" phylum="Nematoda" rank="species" species="annetteae">
<emphasis id="B928EAB1A8129D5F4A4D5128FCDBF82D" box="[594,805,1914,1938]" italics="true" pageId="21" pageNumber="222">Eutobrilus annetteae</emphasis>
(
<bibRefCitation id="EFCD4B52A8129D5F4B2E512BFBE3F82E" author="Joubert, A. P. &amp; Heyns, J." box="[817,1053,1913,1938]" pageId="21" pageNumber="222" pagination="17 - 26" refId="ref15185" refString="Joubert, A. P. &amp; Heyns, J. (1979) Freshwater nematodes from South Africa. 3. Tobrilus Andrassy, 1959. Journal of the Limnological Society of Southern Africa, 5 (1), 17 - 26. https: // doi. org / 10.1080 / 03779688.1979.9633176" type="journal article" year="1979">Joubert &amp; Heyns, 1979</bibRefCitation>
)
<bibRefCitation id="EFCD4B52A8129D5F4C36512BFB27F82E" author="Tsalolikhin, S. Ya." box="[1065,1241,1913,1938]" pageId="21" pageNumber="222" refId="ref16207" refString="Tsalolikhin, S. Ya. (1983) Nematodes from families Tobrilidae and Tipylidae of all world fauna. Leningrad: Nauka, 232 pp. [in Russian]" type="book" year="1983">Tsalolikhin, 1983</bibRefCitation>
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female A) Anterior region indicating buccal cavity to cardia; B) Whole female specimen, arrow indicating vulva; C) Vulval region showing simple vaginal musculature; D) Labial region showing buccal cavity and two overlapping tooth pockets (
<emphasis id="B928EAB1A8129D5F4C695190FB6CF866" box="[1142,1170,1986,2010]" italics="true" pageId="21" pageNumber="222">T1</emphasis>
and
<emphasis id="B928EAB1A8129D5F4CDD5190FB20F866" box="[1218,1246,1986,2010]" italics="true" pageId="21" pageNumber="222">T2</emphasis>
).
</paragraph>
</caption>
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<paragraph id="8BE336A3A8119D5C48885398FADEF997" blockId="22.[151,1436,1482,1579]" pageId="22" pageNumber="223">
<emphasis id="B928EAB1A8119D5C48885398FEDCFA5F" bold="true" box="[151,290,1482,1507]" pageId="22" pageNumber="223">FIGURE 14.</emphasis>
Female drawings
<taxonomicName id="4C5C4D20A8119D5C49F45399FB88FA5F" authority="(Joubert &amp; Heyns, 1979) Tsalolikhin, 1981" authorityName="Tsalolikhin" authorityYear="1981" baseAuthorityName="Joubert &amp; Heyns" baseAuthorityYear="1979" box="[491,1142,1482,1507]" class="Enoplea" family="Tobrilidae" genus="Neotobrilus" higherTaxonomySource="GBIF" kingdom="Animalia" order="Triplonchida" pageId="22" pageNumber="223" phylum="Nematoda" rank="species" species="ampiei">
<emphasis id="B928EAB1A8119D5C49F45399FD4EFA5E" box="[491,688,1483,1507]" italics="true" pageId="22" pageNumber="223">Neotobrilus ampiei</emphasis>
(
<bibRefCitation id="EFCD4B52A8119D5C4AA05398FC4AFA5F" author="Joubert, A. P. &amp; Heyns, J." box="[703,948,1482,1507]" pageId="22" pageNumber="223" pagination="17 - 26" refId="ref15185" refString="Joubert, A. P. &amp; Heyns, J. (1979) Freshwater nematodes from South Africa. 3. Tobrilus Andrassy, 1959. Journal of the Limnological Society of Southern Africa, 5 (1), 17 - 26. https: // doi. org / 10.1080 / 03779688.1979.9633176" type="journal article" year="1979">Joubert &amp; Heyns, 1979</bibRefCitation>
)
<bibRefCitation id="EFCD4B52A8119D5C4BDC5398FB88FA5F" author="Tsalolikhin, S. Ya." box="[963,1142,1482,1507]" pageId="22" pageNumber="223" pagination="1302 - 1313" refId="ref16176" refString="Tsalolikhin, S. Ya. (1981) Revision of the genus Tobrilus (Nematoda-Tobrilidae). Zoologicheskii Zhurnal, 60, 1302 - 1313. [in Russian]" type="journal article" year="1981">Tsalolikhin, 1981</bibRefCitation>
</taxonomicName>
A) Anterior region showing oesophagus; B) Tail; C) Vulval region showing musculature of vagina; D) Anterior region showing structure of stoma and teeth pockets; E) Vulval region showing part of anterior genital tract with two eggs and full posterior tract with one egg.
</paragraph>
</caption>
<paragraph id="8BE336A3A8119D5C48D8500BFEE1F8D3" blockId="22.[151,1437,1625,2012]" pageId="22" pageNumber="223">
Discussion: The
<taxonomicName id="4C5C4D20A8119D5C499A500BFDF7F9CF" box="[389,521,1625,1651]" class="Enoplea" family="Tobrilidae" genus="Neotobrilus" higherTaxonomySource="GBIF" kingdom="Animalia" order="Triplonchida" pageId="22" pageNumber="223" phylum="Nematoda" rank="genus">
<emphasis id="B928EAB1A8119D5C499A500BFDF7F9CF" box="[389,521,1625,1651]" italics="true" pageId="22" pageNumber="223">Neotobrilus</emphasis>
</taxonomicName>
specimens collected in Telperion Nature Reserve was identified following a key from
<bibRefCitation id="EFCD4B52A8119D5C48CB502FFDD8F92B" author="Tsalolikhin, S. Ya. &amp; Shoshin, A. V." box="[212,550,1661,1687]" pageId="22" pageNumber="223" pagination="59 - 72" refId="ref16238" refString="Tsalolikhin, S. Ya. &amp; Shoshin, A. V. (2009) Riviiw of the genus Neotobrilus Tsalolikhin, 1981 (Nematoda, Enoplida: Tobrilidae). Russian Journal of Nematology, 17 (1), 59 - 72." type="journal article" year="2009">Tsalolikhin &amp; Shoshin (2009)</bibRefCitation>
.
<taxonomicName id="4C5C4D20A8119D5C4A2E502FFCF7F92B" authorityName="Tsalolikhin" authorityYear="1981" baseAuthorityName="Joubert &amp; Heyns" baseAuthorityYear="1979" box="[561,777,1661,1687]" class="Enoplea" family="Tobrilidae" genus="Neotobrilus" higherTaxonomySource="GBIF" kingdom="Animalia" order="Triplonchida" pageId="22" pageNumber="223" phylum="Nematoda" rank="species" species="ampiei">
<emphasis id="B928EAB1A8119D5C4A2E502FFCF7F92B" box="[561,777,1661,1687]" italics="true" pageId="22" pageNumber="223">Neotobrilus ampiei</emphasis>
</taxonomicName>
is distinguished from all other species in this genus by the uniquely sculptured eggshells in the females and by the closely positioned supplements 2 and
<quantity id="4CA49B46A8119D5C4CA450F3FB18F907" box="[1211,1254,1697,1723]" metricMagnitude="-2" metricUnit="m" metricValue="7.62" pageId="22" pageNumber="223" unit="in" value="3.0">3 in</quantity>
males. The current female specimens exactly matches those of the original description by
<bibRefCitation id="EFCD4B52A8119D5C4BF85097FB04F963" author="Joubert, A. P. &amp; Heyns, J." box="[999,1274,1733,1760]" pageId="22" pageNumber="223" pagination="17 - 26" refId="ref15185" refString="Joubert, A. P. &amp; Heyns, J. (1979) Freshwater nematodes from South Africa. 3. Tobrilus Andrassy, 1959. Journal of the Limnological Society of Southern Africa, 5 (1), 17 - 26. https: // doi. org / 10.1080 / 03779688.1979.9633176" type="journal article" year="1979">Joubert &amp; Heyns (1979)</bibRefCitation>
. However, the males differ slightly in: spicule length (6172 μm
<emphasis id="B928EAB1A8119D5C4ACF50B8FD19F8BF" box="[720,743,1770,1795]" italics="true" pageId="22" pageNumber="223">vs</emphasis>
6980 μm) and c-value (9.2613.26
<emphasis id="B928EAB1A8119D5C4C9250B8FB5AF8BF" box="[1165,1188,1770,1795]" italics="true" pageId="22" pageNumber="223">vs</emphasis>
8.49.9).
<taxonomicName id="4C5C4D20A8119D5C4D0750BBFF1DF89B" authorityName="Tsalolikhin" authorityYear="1981" baseAuthorityName="Joubert &amp; Heyns" baseAuthorityYear="1979" class="Enoplea" family="Tobrilidae" genus="Neotobrilus" higherTaxonomySource="GBIF" kingdom="Animalia" order="Triplonchida" pageId="22" pageNumber="223" phylum="Nematoda" rank="species" species="ampiei">
<emphasis id="B928EAB1A8119D5C4D0750BBFF1DF89B" italics="true" pageId="22" pageNumber="223">Neotobrilus ampiei</emphasis>
</taxonomicName>
is considered endemic as the only reports are from the
<typeStatus id="54E78801A8119D5C4B51515CFC80F894" box="[846,894,1806,1832]" pageId="22" pageNumber="223">type</typeStatus>
locality in the
<collectingRegion id="4998F841A8119D5C4C39515CFAD8F89B" box="[1062,1318,1805,1832]" country="South Africa" name="Eastern Cape" pageId="22" pageNumber="223">Eastern Cape Province</collectingRegion>
and a second population in the
<collectingRegion id="4998F841A8119D5C49925163FD86F8F7" box="[397,632,1841,1868]" country="South Africa" name="North West" pageId="22" pageNumber="223">North West Province</collectingRegion>
described by
<bibRefCitation id="EFCD4B52A8119D5C4B0B5163FBD8F8F7" author="Joubert, A. P. &amp; Heyns, J." box="[788,1062,1841,1868]" pageId="22" pageNumber="223" pagination="17 - 26" refId="ref15185" refString="Joubert, A. P. &amp; Heyns, J. (1979) Freshwater nematodes from South Africa. 3. Tobrilus Andrassy, 1959. Journal of the Limnological Society of Southern Africa, 5 (1), 17 - 26. https: // doi. org / 10.1080 / 03779688.1979.9633176" type="journal article" year="1979">Joubert &amp; Heyns (1979)</bibRefCitation>
. This is only the second report of this species.
</paragraph>
<paragraph id="8BE336A3A8119D5C48D8512BFD96F867" blockId="22.[151,1437,1625,2012]" pageId="22" pageNumber="223">
Locality and specimens examined: Specimens of
<taxonomicName id="4C5C4D20A8119D5C4AF25128FCA7F82F" authorityName="Tsalolikhin" authorityYear="1981" baseAuthorityName="Joubert &amp; Heyns" baseAuthorityYear="1979" box="[749,857,1914,1939]" class="Enoplea" family="Tobrilidae" genus="Neotobrilus" higherTaxonomySource="GBIF" kingdom="Animalia" order="Triplonchida" pageId="22" pageNumber="223" phylum="Nematoda" rank="species" species="ampiei">
<emphasis id="B928EAB1A8119D5C4AF25128FCA7F82F" box="[749,857,1914,1939]" italics="true" pageId="22" pageNumber="223">N. ampiei</emphasis>
</taxonomicName>
were found at the Waterhole (MP00031) site (
<tableCitation id="C6DE0318A8119D5C4D43512BFF5DF80B" captionStart="TABLE 1" captionStartId="3.[151,244,789,816]" captionTargetBox="[165,2009,835,1386]" captionText="TABLE 1. Detailed description of the sites selected for the water-substrate samples taken from the Telperion Nature Reserve." httpUri="http://table.plazi.org/id/DF23662BA8049D4948885547FA27FC8C" pageId="22" pageNumber="223" tableUuid="DF23662BA8049D4948885547FA27FC8C">Table 1</tableCitation>
) and are deposited in the National Collection of Nematodes (ARC—Plant Health and Protection, Biosystematics, Pretoria) on slide numbers 5059150599.
</paragraph>
</subSubSection>
</treatment>
</document>